{"id":10494,"date":"2025-06-30T15:47:05","date_gmt":"2025-06-30T07:47:05","guid":{"rendered":"http:\/\/localhost\/?p=10494"},"modified":"2025-12-21T01:03:36","modified_gmt":"2025-12-20T17:03:36","slug":"k562%e7%bb%86%e8%83%9e%e7%b3%bb%e4%b8%adupr%e8%af%b1%e5%af%bc%e4%b8%adtg%e5%92%8ctm%e7%9a%84%e6%b5%93%e5%ba%a6%e5%92%8c%e6%8c%81%e7%bb%ad%e6%97%b6%e9%97%b4%e7%a0%94%e7%a9%b6%e6%8a%a5%e5%91%8a","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=10494","title":{"rendered":"K562\u7ec6\u80de\u7cfb\u4e2dUPR\u8bf1\u5bfc\u4e2dTG\u548cTM\u7684\u6d53\u5ea6\u548c\u6301\u7eed\u65f6\u95f4\u7814\u7a76\u62a5\u544a"},"content":{"rendered":"\n
\n

From google research: https:\/\/g.co\/gemini\/share\/5014de990df2<\/a><\/p>\n<\/blockquote>\n\n\n\n

\u6267\u884c\u6458\u8981<\/h2>\n\n\n\n

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\u5c3d\u7ba1\u73b0\u6709\u6587\u732e\u4e2d\u76f4\u63a5\u9488\u5bf9K562\u7ec6\u80de\u7684\u7cbe\u786e\u6570\u636e\u6709\u9650\uff0c\u4f46\u7814\u7a76\u8bc1\u5b9eTG\u548cTM\u5747\u80fd\u6709\u6548\u8bf1\u5bfcK562\u7ec6\u80de\u7684XBP1\u526a\u63a5\u548cUPR\u6fc0\u6d3b\uff0c\u5c24\u5176\u662f\u57288\u5c0f\u65f6\u7684\u66b4\u9732\u7a97\u53e3\u671f\u5185\u3002\u901a\u8fc7\u5bf9\u5176\u4ed6\u7ecf\u8fc7\u5145\u5206\u8868\u5f81\u7684\u4eba\u7c7b\u7ec6\u80de\u7cfb\u6570\u636e\u7684\u63a8\u65ad\uff0c\u5efa\u8bae\u786b\u666e\u897f\u52a0\u7684\u6709\u6548\u6d53\u5ea6\u8303\u56f4\u4e3a500 nM\u81f31 \u00b5M\uff0c\u8863\u9709\u7d20\u4e3a4-5 \u00b5g\/mL\uff0c\u901a\u5e38\u6301\u7eed8-12\u5c0f\u65f6\u3002<\/p>\n\n\n\n

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1. \u672a\u6298\u53e0\u86cb\u767d\u53cd\u5e94 (UPR) \u7b80\u4ecb<\/h2>\n\n\n\n

1.1 \u5185\u8d28\u7f51\u5e94\u6fc0\u4e0eUPR\u901a\u8def<\/h3>\n\n\n\n

\u5185\u8d28\u7f51\uff08ER\uff09\u662f\u771f\u6838\u7ec6\u80de\u4e2d\u4e00\u4e2a\u81f3\u5173\u91cd\u8981\u7684\u7ec6\u80de\u5668\uff0c\u4e3b\u8981\u8d1f\u8d23\u5206\u6ccc\u86cb\u767d\u548c\u8de8\u819c\u86cb\u767d\u7684\u5408\u6210\u3001\u6298\u53e0\u3001\u4fee\u9970\u548c\u8d28\u91cf\u63a7\u5236 1<\/sup>\u3002\u5f53\u5185\u8d28\u7f51\u7684\u86cb\u767d\u8d28\u6298\u53e0\u80fd\u529b\u88ab\u8d85\u8d1f\u8377\uff0c\u5bfc\u81f4\u672a\u6298\u53e0\u6216\u9519\u8bef\u6298\u53e0\u7684\u86cb\u767d\u8d28\u5728\u8154\u5185\u79ef\u7d2f\u65f6\uff0c\u5c31\u4f1a\u53d1\u751f\u5185\u8d28\u7f51\u5e94\u6fc0 3<\/sup>\u3002\u8fd9\u79cd\u6270\u52a8\u53ef\u7531\u591a\u79cd\u751f\u7406\u548c\u75c5\u7406\u523a\u6fc0\u5f15\u8d77\uff0c\u5305\u62ec\u8425\u517b\u5265\u593a\u3001\u7f3a\u6c27\u4ee5\u53ca\u7ec6\u80de\u6bd2\u6027\u5316\u5408\u7269\u7684\u4f5c\u7528 3<\/sup>\u3002<\/p>\n\n\n\n

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1.2 IRE1-XBP1\u8f74\uff1aUPR\u7684\u6838\u5fc3\u4fe1\u53f7\u5206\u652f<\/h3>\n\n\n\n

\u5728\u4e09\u79cdUPR\u5206\u652f\u4e2d\uff0cIRE1-XBP1\u8f74\u662f\u4ecb\u5bfc\u5185\u8d28\u7f51\u5e94\u6fc0\u9002\u5e94\u6027\u53cd\u5e94\u7684\u9ad8\u5ea6\u4fdd\u5b88\u4e14\u5173\u952e\u7684\u901a\u8def 1<\/sup>\u3002\u5f53\u672a\u6298\u53e0\u86cb\u767d\u8d28\u5728\u5185\u8d28\u7f51\u8154\u5185\u79ef\u7d2f\u65f6\uff0cIRE1\u03b1\uff08IRE1\u7684\u54fa\u4e73\u52a8\u7269\u540c\u6e90\u7269\uff09\u4f1a\u53d1\u751f\u4e8c\u805a\u5316\u548c\u5be1\u805a\u5316\uff0c\u4ece\u800c\u5bfc\u81f4\u5176\u53cd\u5f0f\u81ea\u78f7\u9178\u5316\u5e76\u6fc0\u6d3b\u5176\u80de\u8d28\u5185\u5207\u6838\u9178\u9176\u7ed3\u6784\u57df 7<\/sup>\u3002\u8fd9\u79cd\u88ab\u6fc0\u6d3b\u7684IRE1\u03b1\u5185\u5207\u6838\u9178\u9176\u7279\u5f02\u6027\u5730\u9776\u5411\u5e76\u52a0\u5de5\u7f16\u7801X-\u76d2\u7ed3\u5408\u86cb\u767d1\uff08XBP1\uff09\u7684mRNA 1<\/sup>\u3002<\/p>\n\n\n\n

1.3 XBP1\u526a\u63a5\u4f5c\u4e3aIRE1\u6fc0\u6d3b\u7684\u6807\u5fd7<\/h3>\n\n\n\n

\u672a\u526a\u63a5\u7684XBP1 mRNA\uff08XBP1u\uff09\u5728\u5176\u7f16\u7801\u5e8f\u5217\u4e2d\u542b\u6709\u4e00\u4e2a\u72ec\u7279\u768426\u6838\u82f7\u9178\uff08nt\uff09\u5185\u542b\u5b50 2<\/sup>\u3002\u88ab\u6fc0\u6d3b\u7684IRE1\u03b1\u4f1a\u5207\u9664\u8fd9\u4e2a\u5185\u542b\u5b50\uff0c\u5bfc\u81f4\u7ffb\u8bd1\u79fb\u7801\u3002\u7531\u6b64\u4ea7\u751f\u7684XBP1\u526a\u63a5\u5f62\u5f0f\uff08XBP1s\uff09\u7f16\u7801\u4e00\u4e2a\u5f3a\u6548\u4e14\u529f\u80fd\u72ec\u7279\u7684\u8f6c\u5f55\u56e0\u5b50 1<\/sup>\u3002XBP1s\u968f\u540e\u8f6c\u8fd0\u5230\u7ec6\u80de\u6838\uff0c\u5728\u90a3\u91cc\u6fc0\u6d3b\u4f17\u591aUPR\u9776\u57fa\u56e0\u7684\u8f6c\u5f55\u3002\u8fd9\u4e9b\u57fa\u56e0\u53c2\u4e0e\u589e\u5f3a\u5185\u8d28\u7f51\u86cb\u767d\u8d28\u6298\u53e0\u80fd\u529b\uff08\u4f8b\u5982\uff0c\u5185\u8d28\u7f51\u4f34\u4fa3\u86cb\u767d\uff09\u3001\u4fc3\u8fdb\u5185\u8d28\u7f51\u76f8\u5173\u964d\u89e3\uff08ERAD\uff09\u7ec4\u5206\u4ee5\u53ca\u652f\u6301\u5185\u8d28\u7f51\u751f\u7269\u53d1\u751f\u548c\u78f7\u8102\u5408\u6210 1<\/sup>\u3002<\/p>\n\n\n\n

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2. K562\u7ec6\u80de\u7cfb\u5728UPR\u7814\u7a76\u4e2d\u7684\u5e94\u7528<\/h2>\n\n\n\n

2.1 K562\u7ec6\u80de\u7684\u7279\u6027\u548c\u76f8\u5173\u6027<\/h3>\n\n\n\n

K562\u7ec6\u80de\u7cfb\u662f\u4e00\u79cd\u6210\u719f\u7684\u4eba\u7c7b\u6c38\u751f\u5316\u9ad3\u7cfb\u767d\u8840\u75c5\u7ec6\u80de\u7cfb\uff0c\u6700\u521d\u6765\u6e90\u4e8e\u4e00\u540d\u5904\u4e8e\u6025\u53d8\u671f\u6162\u6027\u9ad3\u7cfb\u767d\u8840\u75c5\uff08CML\uff09\u60a3\u8005\u7684\u80f8\u8154\u79ef\u6db2 15<\/sup>\u3002\u5b83\u5728\u8840\u6db2\u5b66\u764c\u75c7\u7814\u7a76\u4e2d\u88ab\u5e7f\u6cdb\u7528\u4f5c\u6a21\u578b\u7cfb\u7edf 15<\/sup>\u3002K562\u7ec6\u80de\u901a\u5e38\u5728\u8865\u5145\u6709\u80ce\u725b\u8840\u6e05\uff08FBS\uff09\u3001\u9752\u9709\u7d20-\u94fe\u9709\u7d20\u548c\u4e19\u916e\u9178\u94a0\u7684RPMI 1640\u57f9\u517b\u57fa\u4e2d\u57f9\u517b 16<\/sup>\u3002\u5b83\u4eec\u8868\u73b0\u51fa\u5feb\u901f\u7684\u589e\u6b96\u7387\uff0c\u5728\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\u5bc6\u5ea6\u5927\u7ea6\u6bcf24\u5c0f\u65f6\u7ffb\u500d 17<\/sup>\u3002<\/p>\n\n\n\n

UPR\u5728\u764c\u75c7\u751f\u7269\u5b66\u4e2d\uff0c\u5305\u62ec\u767d\u8840\u75c5\uff0c\u5177\u6709\u7279\u6b8a\u7684\u76f8\u5173\u6027\u3002\u8fd9\u662f\u56e0\u4e3a\u8f6c\u5316\u7ec6\u80de\u7531\u4e8e\u5feb\u901f\u589e\u6b96\u3001\u4ee3\u8c22\u6539\u53d8\u4ee5\u53ca\u901a\u5e38\u4e0d\u5229\u7684\u5fae\u73af\u5883\uff0c\u7ecf\u5e38\u7ecf\u5386\u86cb\u767d\u8d28\u7a33\u6001\u7684\u7d0a\u4e71 5<\/sup>\u3002\u5728\u764c\u7ec6\u80de\u4e2d\uff0cUPR\u53ef\u4ee5\u53d1\u6325\u590d\u6742\u4e14\u901a\u5e38\u662f\u53cc\u91cd\u7684\u4f5c\u7528\uff1a\u6700\u521d\u4f5c\u4e3a\u4e00\u79cd\u4fc3\u751f\u5b58\u673a\u5236\uff0c\u652f\u6301\u80bf\u7624\u7ec6\u80de\u7684\u9002\u5e94\u548c\u5bf9\u5316\u7597\u7684\u62b5\u6297\uff0c\u4f46\u5728\u4e25\u91cd\u6216\u957f\u65f6\u95f4\u7684\u5e94\u6fc0\u6761\u4ef6\u4e0b\uff0c\u5b83\u53ef\u80fd\u8f6c\u53d8\u4e3a\u4fc3\u51cb\u4ea1\u901a\u8def\uff0c\u5bfc\u81f4\u7ec6\u80de\u6b7b\u4ea1 5<\/sup>\u3002<\/p>\n\n\n\n

K562\u7ec6\u80de\u7cfb\u4f5c\u4e3a\u767d\u8840\u75c5\u7814\u7a76\u7684\u91cd\u8981\u6a21\u578b\uff0c\u5176UPR\u7684\u7cbe\u786e\u8bf1\u5bfc\u5177\u6709\u91cd\u8981\u7684\u6cbb\u7597\u610f\u4e49\u3002K562\u7ec6\u80de\u662f\u4e00\u79cd\u4eba\u7c7b\u767d\u8840\u75c5\u7ec6\u80de\u7cfb 16<\/sup>\u3002UPR\u5728\u764c\u7ec6\u80de\uff08\u5305\u62ec\u767d\u8840\u75c5\u7ec6\u80de\uff09\u4e2d\u626e\u6f14\u7740\u590d\u6742\u4e14\u901a\u5e38\u662f\u4fc3\u751f\u5b58\u7684\u89d2\u8272\uff0c\u4f46\u5728\u4e25\u91cd\u6216\u957f\u65f6\u95f4\u7684\u5e94\u6fc0\u4e0b\u4e5f\u80fd\u89e6\u53d1\u7ec6\u80de\u6b7b\u4ea1 5<\/sup>\u3002\u56e0\u6b64\uff0c\u5728K562\u7ec6\u80de\u4e2d\u7cbe\u786e\u8bf1\u5bfcUPR\uff08\u548cXBP1\u526a\u63a5\uff09\u4e0d\u4ec5\u5bf9\u57fa\u7840\u7814\u7a76\u81f3\u5173\u91cd\u8981\uff0c\u800c\u4e14\u5bf9\u4e8e\u7406\u89e3\u767d\u8840\u75c5\u53d1\u75c5\u673a\u5236\u548c\u5f00\u53d1\u65b0\u578b\u6cbb\u7597\u7b56\u7565\u5177\u6709\u76f4\u63a5\u7684\u8f6c\u5316\u610f\u4e49\u3002\u4f8b\u5982\uff0c\u5982\u679c\u6cbb\u7597\u5242\u65e8\u5728\u8bf1\u5bfc\u767d\u8840\u75c5\u7ec6\u80de\u7684\u7ec8\u672bUPR\uff0c\u90a3\u4e48\u6700\u4f73\u7684TG\/TM\u6d53\u5ea6\u548c\u6301\u7eed\u65f6\u95f4\u5c06\u662f\u90a3\u4e9b\u80fd\u5c06\u7ec6\u80de\u63a8\u5411\u51cb\u4ea1\u800c\u975e\u4ec5\u4ec5\u6fc0\u6d3b\u9002\u5e94\u6027\u3001\u4fc3\u751f\u5b58UPR\u7684\u6761\u4ef6\u3002\u8fd9\u51f8\u663e\u4e86K562\u4f5c\u4e3a\u7814\u7a76\u5185\u8d28\u7f51\u5e94\u6fc0\u6cbb\u7597\u64cd\u7eb5\u7684\u5173\u952e\u6a21\u578b\u3002<\/p>\n\n\n\n

3. \u786b\u666e\u897f\u52a0 (TG) \u5728K562\u7ec6\u80deUPR\u8bf1\u5bfc\u4e2d\u7684\u5e94\u7528<\/h2>\n\n\n\n

3.1 \u4f5c\u7528\u673a\u5236\u53ca\u5176\u5bf9\u5185\u8d28\u7f51\u9499\u7a33\u6001\u7684\u5f71\u54cd<\/h3>\n\n\n\n

\u786b\u666e\u897f\u52a0\uff08Thapsigargin, TG\uff09\u662f\u516c\u8ba4\u7684\u5f3a\u6548\u5185\u8d28\u7f51\u5e94\u6fc0\u8bf1\u5bfc\u5242 3<\/sup>\u3002\u5176\u4e3b\u8981\u4f5c\u7528\u673a\u5236\u662f\u7279\u5f02\u6027\u6291\u5236\u808c\u6d46\u7f51\u6216\u5185\u8d28\u7f51Ca2+ ATP\u9176\uff08SERCA\uff09\u6cf5 3<\/sup>\u3002\u901a\u8fc7\u963b\u65adSERCA\uff0cTG\u963b\u6b62\u9499\u79bb\u5b50\u91cd\u65b0\u6444\u53d6\u5230\u5185\u8d28\u7f51\u8154\u5185\uff0c\u5bfc\u81f4\u5185\u8d28\u7f51\u9499\u50a8\u5b58\u8017\u5c3d\u3002\u8fd9\u79cd\u5185\u8d28\u7f51\u9499\u7a33\u6001\u7684\u7834\u574f\u4f1a\u635f\u5bb3\u9499\u4f9d\u8d56\u6027\u5185\u8d28\u7f51\u4f34\u4fa3\u86cb\u767d\uff08\u5982\u9499\u7f51\u86cb\u767d\uff09\u7684\u529f\u80fd\uff0c\u8fd9\u4e9b\u4f34\u4fa3\u86cb\u767d\u5bf9\u4e8e\u65b0\u751f\u591a\u80bd\u7684\u6b63\u786e\u6298\u53e0\u548c\u52a0\u5de5\u81f3\u5173\u91cd\u8981 3<\/sup>\u3002\u7531\u6b64\u5bfc\u81f4\u7684\u672a\u6298\u53e0\u86cb\u767d\u8d28\u79ef\u7d2f\u968f\u540e\u89e6\u53d1UPR\u3002<\/p>\n\n\n\n

3.2 K562\u7ec6\u80de\u4e2dXBP1\u526a\u63a5\u7684\u62a5\u544a\u6d53\u5ea6\u548c\u6301\u7eed\u65f6\u95f4<\/h3>\n\n\n\n

\u5728\u73b0\u6709\u7814\u7a76\u6587\u732e\u4e2d\uff0c\u76f4\u63a5\u3001\u7cbe\u786e\u91cf\u5316\u7528\u4e8e\u8bf1\u5bfcK562\u7ec6\u80deXBP1\u526a\u63a5\u7684\u786b\u666e\u897f\u52a0\u6d53\u5ea6\u6570\u636e\u975e\u5e38\u6709\u9650\u3002\u591a\u9879\u7814\u7a76\u8bc1\u5b9e\uff0c\u786b\u666e\u897f\u52a0\u4f5c\u4e3a\u4e00\u79cd\u201c\u771f\u6b63\u7684\u5185\u8d28\u7f51\u5e94\u6fc0\u8bf1\u5bfc\u5242\u201d\uff0c\u5728K562\u7ec6\u80de\u4e2d\u5bfc\u81f4\u4e86PARP\u88c2\u89e3\u548c\u7ec6\u80de\u6b7b\u4ea1\uff0c\u8fd9\u4e9b\u7ec6\u80de\u7ecf\u8fc7\u4e8615\u5c0f\u65f6 19<\/sup> \u621618\u5c0f\u65f6 19<\/sup> \u7684\u5b75\u80b2\u3002\u5c3d\u7ba1\u5728\u8fd9\u4e9b\u7814\u7a76\u4e2d\u68c0\u6d4b\u5230\u4e86XBP1 mRNA\u526a\u63a5\uff0c\u4f46\u5e76\u672a\u660e\u786e\u6307\u51fa\u7528\u4e8e\u8fd9\u4e9b\u89c2\u5bdf\u7684\u786b\u666e\u897f\u52a0\u5177\u4f53\u6d53\u5ea6\u3002\u4e00\u9879\u5173\u952e\u7814\u7a76\u660e\u786e\u6307\u51fa\uff0c\u7528\u786b\u666e\u897f\u52a0\u5904\u7406K562\u7ec6\u80de8\u5c0f\u65f6\u540e\uff0cXBP1\u8868\u8fbe\u548c\u526a\u63a5\u72b6\u6001\u663e\u793a\u51fa\u201c\u5f3a\u70c8\u7684UPR\u6fc0\u6d3b\u201d 9<\/sup>\u3002\u7136\u800c\uff0c\u8be5\u5b9e\u9a8c\u4e2d\u4f7f\u7528\u7684\u786b\u666e\u897f\u52a0\u7cbe\u786e\u6d53\u5ea6\u540c\u6837\u672a\u88ab\u63d0\u4f9b\u3002<\/p>\n\n\n\n

3.3 \u5176\u4ed6\u4eba\u7c7b\u7ec6\u80de\u7cfb\u7684\u53c2\u8003\u6570\u636e<\/h3>\n\n\n\n

\u9274\u4e8eK562\u7ec6\u80de\u7cbe\u786e\u6d53\u5ea6\u6570\u636e\u7684\u7a00\u7f3a\u6027\uff0c\u5176\u4ed6\u4eba\u7c7b\u7ec6\u80de\u7cfb\u4e2d\u8fdb\u884c\u7684\u5b9e\u9a8c\u63d0\u4f9b\u4e86\u5b9d\u8d35\u7684\u53c2\u8003\uff0c\u6709\u52a9\u4e8e\u5efa\u7acb\u53ef\u80fd\u7684\u6709\u6548\u8303\u56f4\u3002<\/p>\n\n\n\n

\u5728\u6c38\u751f\u5316\u4ebaB\u7ec6\u80de\u4e2d\uff0c500 nM\u7684\u786b\u666e\u897f\u52a0\u80fd\u6709\u6548\u8bf1\u5bfc\u5185\u8d28\u7f51\u5e94\u6fc0\uff0c\u5e76\u89c2\u5bdf\u5230XBP1\u526a\u63a5\u4f5c\u4e3a\u5176\u6807\u5fd7 3\u3002\u5728\u8fd9\u4e9b\u7ec6\u80de\u4e2d\uff0cUPR\u76f8\u5173\u57fa\u56e0\u7684\u8868\u8fbe\u5728\u5904\u7406\u540e8\u5c0f\u65f6\u5185\u5927\u90e8\u5206\u88ab\u8bf1\u5bfc\u6216\u6291\u5236 3\u3002<\/p>\n\n\n\n

\u5728\u591a\u53d1\u6027\u9aa8\u9ad3\u7624\u7ec6\u80de\u7cfb\uff08U266, H929, MM1s\uff09\u4e2d\uff0c\u4f7f\u7528\u4e86\u66f4\u9ad8\u6d53\u5ea6\u768410 \u00b5M\u786b\u666e\u897f\u52a0\uff0c\u5bfc\u81f4\u201c\u65e9\u671fXBP1\u526a\u63a5\u201d 20\u3002\u8fd9\u8868\u660e\u5242\u91cf\u4e0e\u53cd\u5e94\u4e4b\u95f4\u5b58\u5728\u5173\u7cfb\uff0c\u8f83\u9ad8\u6d53\u5ea6\u53ef\u80fd\u5f15\u53d1\u66f4\u5feb\u6216\u66f4\u5f3a\u7684\u526a\u63a5\u53cd\u5e94\u3002<\/p>\n\n\n\n

\u5728Huh-7\u7ec6\u80de\u4e2d\uff0c1 \u00b5M\u7684\u786b\u666e\u897f\u52a0\uff08THA\uff09\u88ab\u7528\u4e8e\u6709\u6548\u6291\u5236SARS-CoV-2\u8fdb\u5165\u5bbf\u4e3b\u7ec6\u80de\uff0c\u4e14\u65e0\u7ec6\u80de\u6bd2\u6027 4\u3002\u7136\u800c\uff0c\u5728\u8fd9\u79cd\u7279\u5b9a\u7684\u5b9e\u9a8c\u80cc\u666f\u4e0b\uff08\u8f6c\u5bfc\u7ec6\u80de\uff09\uff0c\u636e\u62a5\u9053IRE1-XBP1s\u901a\u8def\u5728THA\u5904\u7406\u540e\u88ab<\/p>\n\n\n\n

\u4e0b\u8c03<\/em> 4<\/sup>\uff0c\u8fd9\u662f\u4e00\u4e2a\u91cd\u8981\u7684\u7ec6\u5fae\u5dee\u522b\uff0c\u8868\u660eUPR\u7ed3\u679c\u5177\u6709\u80cc\u666f\u4f9d\u8d56\u6027\u3002<\/p>\n\n\n\n

\u5728\u5c0f\u9f20\u80da\u80ce\u6210\u7ea4\u7ef4\u7ec6\u80de\uff08MEFs\uff09\u548cHep G2\u7ec6\u80de\u7684\u7814\u7a76\u4e2d\uff0c\u540c\u6837\u4f7f\u7528\u4e861 \u00b5M\u7684\u786b\u666e\u897f\u52a0\uff0c\u89c2\u5bdf\u5230XBP1\u526a\u63a5\u5728\u6570\u5c0f\u65f6\uff08MEFs\uff09\u62166\u5c0f\u65f6\uff08Hep G2\uff09\u540e\u8fbe\u5230\u6700\u5927\u6c34\u5e73 1\u3002<\/p>\n\n\n\n

\u6839\u636e\u4e0a\u8ff0\u4fe1\u606f\uff0c\u53ef\u4ee5\u63a8\u65adK562\u7ec6\u80de\u7684\u6700\u4f73\u6d53\u5ea6\u8303\u56f4\u3002\u5c3d\u7ba1K562\u7ec6\u80de\u4e2d\u7f3a\u4e4f\u76f4\u63a5\u7684\u786b\u666e\u897f\u52a0\u6d53\u5ea6\u6570\u636e\uff0c\u4f46\u73b0\u6709\u8bc1\u636e\u8bc1\u5b9e\u786b\u666e\u897f\u52a0\u786e\u5b9e\u80fd\u8bf1\u5bfcK562\u7ec6\u80de\u7684XBP1\u526a\u63a5 9<\/sup>\u3002\u4e3a\u4e86\u5f25\u8865\u8fd9\u4e00\u6570\u636e\u7a7a\u767d\uff0c\u53ef\u4ee5\u53c2\u8003\u5176\u4ed6\u4eba\u7c7b\u7ec6\u80de\u7cfb\u7684\u6570\u636e\u6765\u63a8\u65ad\u4e00\u4e2a\u5408\u7406\u7684\u5de5\u4f5c\u8303\u56f4\u3002\u6c38\u751f\u5316B\u7ec6\u80de\u4e2d500 nM\u7684\u6d53\u5ea6\u57288\u5c0f\u65f6\u5185\u8bf1\u5bfc\u4e86XBP1\u526a\u63a5\u548c\u666e\u904d\u7684UPR\u57fa\u56e0\u53d8\u5316 3<\/sup>\uff0c\u8fd9\u4e00\u6301\u7eed\u65f6\u95f4\u4e0eK562\u7ec6\u80de\u4e2d\u89c2\u5bdf\u5230\u7684\u201c\u5f3a\u70c8UPR\u6fc0\u6d3b\u201d\u76f8\u7b26 9<\/sup>\u3002\u800c10 \u00b5M\u7684\u6d53\u5ea6\u5728\u9aa8\u9ad3\u7624\u7ec6\u80de\u4e2d\u8bf1\u5bfc\u4e86\u201c\u65e9\u671f\u526a\u63a5\u201d 20<\/sup>\uff0c\u8fd9\u53ef\u80fd\u8868\u660e\u5176\u4f5c\u7528\u66f4\u5feb\u6216\u66f4\u663e\u8457\u3002\u7efc\u5408\u8fd9\u4e9b\u89c2\u5bdf\uff0c\u5bf9\u4e8eK562\u7ec6\u80de\uff0c\u5efa\u8bae\u8d77\u59cb\u6d53\u5ea6\u8303\u56f4\u4e3a<\/p>\n\n\n\n

500 nM\u81f31 \u00b5M<\/strong>\uff0c\u5904\u7406\u6301\u7eed\u65f6\u95f4\u4e3a 6-15\u5c0f\u65f6<\/strong>\uff0c\u8fd9\u5f88\u53ef\u80fd\u6709\u6548\u8bf1\u5bfcXBP1\u526a\u63a5\u3002\u8f83\u9ad8\u6d53\u5ea6\u53ef\u80fd\u4f1a\u5bfc\u81f4\u66f4\u5feb\u6216\u66f4\u5f3a\u7684\u53cd\u5e94\uff0c\u4f46\u4e5f\u4f34\u968f\u7740\u7ec6\u80de\u6bd2\u6027\u98ce\u9669\u589e\u52a0\uff0c\u56e0\u6b64\u5fc5\u987b\u4ed4\u7ec6\u76d1\u6d4b\u3002\u56e0\u6b64\uff0c\u5f3a\u70c8\u5efa\u8bae\u5bf9K562\u7ec6\u80de\u8fdb\u884c\u7ecf\u9a8c\u6027\u7684\u5242\u91cf-\u53cd\u5e94\u548c\u65f6\u95f4\u8fdb\u7a0b\u4f18\u5316\uff0c\u4ee5\u786e\u5b9aUPR\u8bf1\u5bfc\u548c\u7ec6\u80de\u6d3b\u529b\u4e4b\u95f4\u7684\u6700\u4f73\u5e73\u8861\u3002<\/p>\n\n\n\n

\u88681\uff1aK562\u7ec6\u80de\u53ca\u76f8\u5173\u4eba\u7c7b\u7ec6\u80de\u7cfb\u4e2d\u786b\u666e\u897f\u52a0\u8bf1\u5bfcXBP1\u526a\u63a5\u7684\u6d53\u5ea6\u548c\u6301\u7eed\u65f6\u95f4\u603b\u7ed3<\/strong><\/p>\n\n\n\n

\u7ec6\u80de\u7cfb<\/td>\u786b\u666e\u897f\u52a0\u6d53\u5ea6<\/td>\u5904\u7406\u6301\u7eed\u65f6\u95f4<\/td>\u89c2\u5bdf\u5230\u7684XBP1\u526a\u63a5\/UPR\u6fc0\u6d3b<\/td>\u5907\u6ce8<\/td>\u53c2\u8003\u6587\u732e<\/td><\/tr><\/thead>
K562<\/td>\u672a\u660e\u786e<\/td>15 \u5c0f\u65f6<\/td>\u68c0\u6d4b\u5230XBP1 mRNA\u526a\u63a5<\/td>\u5bfc\u81f4PARP\u88c2\u89e3\u548c\u7ec6\u80de\u6b7b\u4ea1<\/td>19<\/sup><\/td><\/tr>
K562<\/td>\u672a\u660e\u786e<\/td>18 \u5c0f\u65f6<\/td>\u68c0\u6d4b\u5230XBP1 mRNA\u526a\u63a5<\/td>\u5bfc\u81f4PARP\u88c2\u89e3\u548c\u7ec6\u80de\u6b7b\u4ea1<\/td>19<\/sup><\/td><\/tr>
K562<\/td>\u672a\u660e\u786e<\/td>8 \u5c0f\u65f6<\/td>\u5f3a\u70c8\u7684UPR\u6fc0\u6d3b\uff0cXbp1\u526a\u63a5<\/td><\/td>9<\/sup><\/td><\/tr>
\u6c38\u751f\u5316\u4ebaB\u7ec6\u80de<\/td>500 nM<\/td>8 \u5c0f\u65f6<\/td>\u68c0\u6d4b\u5230XBP1\u526a\u63a5<\/td>\u591a\u6570UPR\u76f8\u5173\u57fa\u56e0\u57288\u5c0f\u65f6\u5185\u8bf1\u5bfc\/\u6291\u5236<\/td>3<\/sup><\/td><\/tr>
\u591a\u53d1\u6027\u9aa8\u9ad3\u7624\u7ec6\u80de<\/td>10 \u00b5M<\/td>\u65e9\u671f<\/td>\u65e9\u671fXBP1\u526a\u63a5<\/td>(U266, H929, MM1s)<\/td>20<\/sup><\/td><\/tr>
Huh-7<\/td>1 \u00b5M<\/td>\u672a\u660e\u786e<\/td>IRE1-XBP1s\u901a\u8def\u4e0b\u8c03<\/td>\u5728\u8f6c\u5bfc\u7ec6\u80de\u4e2d\u89c2\u5bdf\u5230\uff0c\u53ef\u80fd\u5177\u6709\u80cc\u666f\u4f9d\u8d56\u6027\uff1b\u65e0\u7ec6\u80de\u6bd2\u6027<\/td>4<\/sup><\/td><\/tr>
MEFs<\/td>1 \u00b5M<\/td>4 \u5c0f\u65f6<\/td>XBP1\u5b8c\u5168\u526a\u63a5<\/td>\u78f7\u9178\u5316JNK\u6fc0\u6d3b\u65e9\u4e8eXBP1\u526a\u63a5<\/td>21<\/sup><\/td><\/tr>
Hep G2<\/td>1 \u00b5M<\/td>6 \u5c0f\u65f6<\/td>XBP1\u526a\u63a5\u8fbe\u5230\u6700\u5927\u6c34\u5e73<\/td><\/td>1<\/sup><\/td><\/tr>
PBEC<\/td>\u4f4e\u5242\u91cf<\/td>6 \u5c0f\u65f6<\/td>XBP1\u526a\u63a5mRNA\u4e3b\u8981\u8868\u8fbe<\/td>\u7528\u4e8e\u7ef4\u6301\u52a8\u6001\u8303\u56f4\uff0c\u907f\u514dUPR\u9776\u57fa\u56e0\u8868\u8fbe\u5728\u4e25\u91cdER\u5e94\u6fc0\u4e0b\u8fbe\u5230\u9971\u548c\u6548\u5e94<\/td>14<\/sup><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

4. \u8863\u9709\u7d20 (TM) \u5728K562\u7ec6\u80deUPR\u8bf1\u5bfc\u4e2d\u7684\u5e94\u7528<\/h2>\n\n\n\n

4.1 \u4f5c\u7528\u673a\u5236\u53ca\u5176\u5bf9N-\u7cd6\u57fa\u5316\u7684\u5f71\u54cd<\/h3>\n\n\n\n

\u8863\u9709\u7d20\uff08Tunicamycin, TM\uff09\u662f\u53e6\u4e00\u79cd\u5e7f\u6cdb\u4f7f\u7528\u7684\u5185\u8d28\u7f51\u5e94\u6fc0\u836f\u7406\u8bf1\u5bfc\u5242 3<\/sup>\u3002TM\u7279\u5f02\u6027\u6291\u5236N-\u7cd6\u57fa\u5316\uff0c\u8fd9\u662f\u5728\u5185\u8d28\u7f51\u4e2d\u53d1\u751f\u7684\u4e00\u79cd\u5173\u952e\u7684\u7ffb\u8bd1\u540e\u4fee\u9970 3<\/sup>\u3002\u901a\u8fc7\u963b\u6b62\u6b63\u786e\u7684\u7cd6\u57fa\u5316\uff0cTM\u5bfc\u81f4\u672a\u7cd6\u57fa\u5316\u548c\u9519\u8bef\u6298\u53e0\u7684\u86cb\u767d\u8d28\u5728\u5185\u8d28\u7f51\u8154\u5185\u79ef\u7d2f\uff0c\u4ece\u800c\u89e6\u53d1\u5185\u8d28\u7f51\u5e94\u6fc0\u548cUPR 3<\/sup>\u3002<\/p>\n\n\n\n

4.2 K562\u7ec6\u80de\u4e2dXBP1\u526a\u63a5\u7684\u62a5\u544a\u6d53\u5ea6\u548c\u6301\u7eed\u65f6\u95f4<\/h3>\n\n\n\n

\u4e0e\u786b\u666e\u897f\u52a0\u7c7b\u4f3c\uff0c\u76f4\u63a5\u3001\u7cbe\u786e\u7684\u8863\u9709\u7d20\u6d53\u5ea6\u6570\u636e\u7528\u4e8e\u8bf1\u5bfcK562\u7ec6\u80deXBP1\u526a\u63a5\u7684\u62a5\u544a\u6709\u9650\u3002\u4e00\u9879\u7814\u7a76\u660e\u786e\u6307\u51fa\uff0c\u8863\u9709\u7d20\u901a\u8fc7\u534a\u5b9a\u91cf\u65b9\u6cd5\u8bf1\u5bfcK562\u7ec6\u80de\u7684XBP1\u526a\u63a5 22<\/sup>\u3002\u7136\u800c\uff0c\u5728\u8be5\u4e0a\u4e0b\u6587\u4e2d\u5e76\u672a\u63d0\u4f9b\u5177\u4f53\u7684\u6d53\u5ea6\u6216\u6301\u7eed\u65f6\u95f4\u3002\u53e6\u4e00\u9879\u5173\u4e8eK562\u7ec6\u80de\u7684\u7814\u7a76\u63d0\u5230\uff0c\u8863\u9709\u7d20\u5904\u74068\u5c0f\u65f6\u540e\uff0cXBP1\u8868\u8fbe\u548c\u526a\u63a5\u663e\u793a\u51fa\u201c\u5f3a\u70c8\u7684UPR\u6fc0\u6d3b\u201d 9<\/sup>\u3002\u8be5\u89c2\u5bdf\u4e2d\u4f7f\u7528\u7684\u5177\u4f53\u6d53\u5ea6\u4e5f\u672a\u8be6\u7ec6\u8bf4\u660e\u3002<\/p>\n\n\n\n

4.3 \u5176\u4ed6\u4eba\u7c7b\u7ec6\u80de\u7cfb\u7684\u53c2\u8003\u6570\u636e<\/h3>\n\n\n\n

\u5728\u6c38\u751f\u5316\u4ebaB\u7ec6\u80de\u4e2d\uff0c4 \u00b5g\/mL\u7684\u8863\u9709\u7d20\u8bf1\u5bfc\u4e86\u5185\u8d28\u7f51\u5e94\u6fc0\uff0c\u5bfc\u81f4XBP1\u526a\u63a5 3<\/sup>\u3002\u4e0e\u786b\u666e\u897f\u52a0\u7c7b\u4f3c\uff0c\u57fa\u56e0\u8868\u8fbe\u7684\u53d8\u5316\u4e3b\u8981\u57288\u5c0f\u65f6\u5185\u89c2\u5bdf\u5230 3<\/sup>\u3002<\/p>\n\n\n\n

\u5728\u4eba\u547c\u5438\u9053\u4e0a\u76ae\u7ec6\u80de\uff08Calu-3\uff09\u4e2d\uff0c\u4f7f\u7528\u4e865 \u00b5g\/ml\u7684\u8863\u9709\u7d20\uff0c\u65f6\u95f4\u8fdb\u7a0b\u7814\u7a76\u663e\u793asXBP1 mRNA\u6c34\u5e73\u572812\u5c0f\u65f6\u540e\u589e\u52a0\u4e863\u500d\u4ee5\u4e0a\uff0c\u5e76\u57288\u5c0f\u65f6\u65f6\u8fbe\u5230\u6700\u5927\u6c34\u5e73 13\u3002HeLa\u7ec6\u80de\u5728\u75285 \u00b5g\/ml\u8863\u9709\u7d20\u5904\u740620\u5c0f\u65f6\u540e\u4e5f\u663e\u793a\u51faUPR\u6fc0\u6d3b 13\u3002<\/p>\n\n\n\n

HEI-OC1\u7ec6\u80de\uff08\u542c\u89c9\u7ec6\u80de\uff09\u5728\u75285\u300150\u548c100 \u00b5g\/ml\u8863\u9709\u7d20\u5904\u74060\u300112\u300124\u548c48\u5c0f\u65f6\u540e\uff0c\u8868\u73b0\u51fa\u5242\u91cf\u548c\u65f6\u95f4\u4f9d\u8d56\u6027\u7684\u7ec6\u80de\u6b7b\u4ea1 23\u3002<\/p>\n\n\n\n

\u5728\u591a\u53d1\u6027\u9aa8\u9ad3\u7624\u7ec6\u80de\u7cfb\uff08U266, H929, MM1s\uff09\u4e2d\uff0c\u4f7f\u7528\u4e86\u663e\u8457\u66f4\u9ad8\u6d53\u5ea6\u7684100 \u00b5M\u8863\u9709\u7d20\uff0c\u5bfc\u81f4\u65e9\u671fXBP1\u526a\u63a5 20\u3002<\/p>\n\n\n\n

\u5728Huh-7\u7ec6\u80de\u4e2d\uff0c5 \u00b5g\/mL\u7684\u8863\u9709\u7d20\uff08TUN\uff09\u6709\u6548\u6291\u5236\u4e86SARS-CoV-2\u8fdb\u5165\u5bbf\u4e3b\u7ec6\u80de\uff0c\u4e14\u65e0\u7ec6\u80de\u6bd2\u6027\uff0c\u5e76\u4e14\u4e0e\u8be5\u80cc\u666f\u4e0b\u7684THA\u4e0d\u540c\uff0c\u5b83\u672a\u4e0b\u8c03IRE1-XBP1s\u901a\u8def 4\u3002<\/p>\n\n\n\n

\u6839\u636e\u4e0a\u8ff0\u4fe1\u606f\uff0c\u53ef\u4ee5\u63a8\u65adK562\u7ec6\u80de\u4e2d\u8863\u9709\u7d20\u8bf1\u5bfc\u7684\u6709\u6548\u8303\u56f4\u3002\u5c3d\u7ba1K562\u7ec6\u80de\u4e2d\u7f3a\u4e4f\u76f4\u63a5\u7684\u8863\u9709\u7d20\u6d53\u5ea6\u6570\u636e\uff0c\u4f46\u7814\u7a76\u8bc1\u5b9e\u8863\u9709\u7d20\u80fd\u8bf1\u5bfcK562\u7ec6\u80de\u7684XBP1\u526a\u63a5 9<\/sup>\u3002\u5176\u4ed6\u4eba\u7c7b\u7ec6\u80de\u7cfb\u7684\u6570\u636e\u663e\u793a\uff0c4 \u00b5g\/mL\u81f35 \u00b5g\/mL\u7684\u6d53\u5ea6\u57288-12\u5c0f\u65f6\u5185\u6709\u6548 3<\/sup>\u3002\u9aa8\u9ad3\u7624\u7ec6\u80de\u4e2d\u4f7f\u7528\u4e86\u66f4\u9ad8\u7684100 \u00b5M\u6d53\u5ea6 20<\/sup>\u3002\u8003\u8651\u5230K562\u7ec6\u80de\u662f\u767d\u8840\u75c5\u7ec6\u80de\u7cfb\uff0c\u5176\u4ed6\u4eba\u7c7b\u7ec6\u80de\u7cfb\u4e2d\u4f7f\u7528\u7684\u6d53\u5ea6\uff0c\u7279\u522b\u662f\u8f83\u4f4e\u76844-5 \u00b5g\/mL\uff0c\u662f\u4e00\u4e2a\u5408\u7406\u7684\u8d77\u59cb\u70b9\u3002K562\u7ec6\u80de\u4e2d8\u5c0f\u65f6\u7684\u5f3a\u70c8UPR\u6fc0\u6d3b\u6301\u7eed\u65f6\u95f4 9<\/sup> \u4e0eCalu-3\u7ec6\u80de\u4e2d\u89c2\u5bdf\u5230\u7684\u52a8\u529b\u5b66\uff088\u5c0f\u65f6\u8fbe\u5230\u6700\u5927\u526a\u63a5\uff09\u76f8\u7b26 13<\/sup>\u3002\u56e0\u6b64\uff0c<\/p>\n\n\n\n

4-5 \u00b5g\/mL<\/strong> \u7684\u8863\u9709\u7d20\uff0c\u5904\u7406 8-12\u5c0f\u65f6<\/strong>\uff0c\u662f\u8bf1\u5bfcK562\u7ec6\u80deXBP1\u526a\u63a5\u7684\u6709\u529b\u5019\u9009\u65b9\u6848\uff0c\u800c\u66f4\u9ad8\u6d53\u5ea6\u53ef\u80fd\u5bfc\u81f4\u66f4\u4e25\u91cd\u7684\u5e94\u6fc0\u548c\u7ec6\u80de\u6bd2\u6027 23<\/sup>\u3002<\/p>\n\n\n\n

\u88682\uff1aK562\u7ec6\u80de\u53ca\u76f8\u5173\u4eba\u7c7b\u7ec6\u80de\u7cfb\u4e2d\u8863\u9709\u7d20\u8bf1\u5bfcXBP1\u526a\u63a5\u7684\u6d53\u5ea6\u548c\u6301\u7eed\u65f6\u95f4\u603b\u7ed3<\/strong><\/p>\n\n\n\n

\u7ec6\u80de\u7cfb<\/td>\u8863\u9709\u7d20\u6d53\u5ea6<\/td>\u5904\u7406\u6301\u7eed\u65f6\u95f4<\/td>\u89c2\u5bdf\u5230\u7684XBP1\u526a\u63a5\/UPR\u6fc0\u6d3b<\/td>\u5907\u6ce8<\/td>\u53c2\u8003\u6587\u732e<\/td><\/tr><\/thead>
K562<\/td>\u672a\u660e\u786e<\/td>8 \u5c0f\u65f6<\/td>\u5f3a\u70c8\u7684UPR\u6fc0\u6d3b\uff0cXbp1\u526a\u63a5<\/td><\/td>9<\/sup><\/td><\/tr>
K562<\/td>\u672a\u660e\u786e<\/td>\u672a\u660e\u786e<\/td>\u8bf1\u5bfcXBP1\u526a\u63a5<\/td>\u534a\u5b9a\u91cf\u65b9\u6cd5\u68c0\u6d4b<\/td>22<\/sup><\/td><\/tr>
\u6c38\u751f\u5316\u4ebaB\u7ec6\u80de<\/td>4 \u00b5g\/mL<\/td>8 \u5c0f\u65f6<\/td>\u68c0\u6d4b\u5230XBP1\u526a\u63a5<\/td>\u591a\u6570UPR\u76f8\u5173\u57fa\u56e0\u57288\u5c0f\u65f6\u5185\u8bf1\u5bfc\/\u6291\u5236<\/td>3<\/sup><\/td><\/tr>
Calu-3<\/td>5 \u00b5g\/ml<\/td>8 \u5c0f\u65f6<\/td>sXBP1 mRNA\u8fbe\u5230\u6700\u5927\u6c34\u5e73<\/td>sXBP1 mRNA\u572812\u5c0f\u65f6\u540e\u589e\u52a0>3\u500d<\/td>13<\/sup><\/td><\/tr>
HeLa<\/td>5 \u00b5g\/ml<\/td>20 \u5c0f\u65f6<\/td>\u8bf1\u5bfcUPR<\/td><\/td>13<\/sup><\/td><\/tr>
HEI-OC1<\/td>5, 50, 100 \u00b5g\/ml<\/td>12, 24, 48 \u5c0f\u65f6<\/td>\u5242\u91cf\u548c\u65f6\u95f4\u4f9d\u8d56\u6027\u7ec6\u80de\u6b7b\u4ea1<\/td><\/td>23<\/sup><\/td><\/tr>
\u591a\u53d1\u6027\u9aa8\u9ad3\u7624\u7ec6\u80de<\/td>100 \u00b5M<\/td>\u65e9\u671f<\/td>\u65e9\u671fXBP1\u526a\u63a5<\/td>(U266, H929, MM1s)<\/td>20<\/sup><\/td><\/tr>
Huh-7<\/td>5 \u00b5g\/mL<\/td>\u672a\u660e\u786e<\/td>\u672a\u4e0b\u8c03IRE1-XBP1s\u901a\u8def<\/td>\u6709\u6548\u6291\u5236SARS-CoV-2\u8fdb\u5165\uff0c\u65e0\u7ec6\u80de\u6bd2\u6027\uff1b\u4e0eTHA\u4f5c\u7528\u4e0d\u540c<\/td>4<\/sup><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

5. \u786b\u666e\u897f\u52a0\u548c\u8863\u9709\u7d20\u5bf9K562 UPR\u5f71\u54cd\u7684\u6bd4\u8f83\u5206\u6790<\/h2>\n\n\n\n

5.1 UPR\u8bf1\u5bfc\u52a8\u529b\u5b66\u548c\u5e45\u5ea6\u7684\u5f02\u540c<\/h3>\n\n\n\n

\u786b\u666e\u897f\u52a0\uff08TG\uff09\u548c\u8863\u9709\u7d20\uff08TM\uff09\u90fd\u662f\u5f3a\u6548\u7684\u5185\u8d28\u7f51\u5e94\u6fc0\u8bf1\u5bfc\u5242\uff0c\u5747\u80fd\u5bfc\u81f4XBP1\u526a\u63a5\uff0c\u5c3d\u7ba1\u5b83\u4eec\u901a\u8fc7\u4e0d\u540c\u7684\u673a\u5236\u53d1\u6325\u4f5c\u7528 3<\/sup>\u3002\u5728K562\u7ec6\u80de\u4e2d\uff0c\u4e24\u8005\u90fd\u57288\u5c0f\u65f6\u5185\u8bf1\u5bfc\u4e86XBP1\u526a\u63a5 9<\/sup>\u3002\u5728\u4e0d\u540c\u7ec6\u80de\u7cfb\u4e2d\uff0cXBP1\u526a\u63a5\u7684\u52a8\u529b\u5b66\u5bf9\u4e8e\u8fd9\u4e24\u79cd\u836f\u7269\u90fd\u663e\u5f97\u76f8\u5bf9\u8fc5\u901f\uff0c\u901a\u5e38\u5728\u51e0\u5206\u949f\u5185\u5373\u53ef\u68c0\u6d4b\u5230\uff0c\u5e76\u5728\u6570\u5c0f\u65f6\u5185\uff08\u4f8b\u59824-8\u5c0f\u65f6\uff09\u8fbe\u5230\u6700\u5927\u6c34\u5e73 1<\/sup>\u3002<\/p>\n\n\n\n

\u7136\u800c\uff0c\u53cd\u5e94\u7684\u5e45\u5ea6\u548c\u5177\u4f53\u7684\u4e0b\u6e38\u6548\u5e94\u53ef\u80fd\u6709\u6240\u4e0d\u540c\u3002\u4f8b\u5982\uff0c\u5728Huh-7\u7ec6\u80de\u4e2d\uff0c\u636e\u62a5\u9053THA\u4e0b\u8c03\u4e86IRE1-XBP1s\u901a\u8def\uff0c\u800cTUN\u5219\u6ca1\u6709 4<\/sup>\u3002\u8fd9\u4e00\u89c2\u5bdf\u7a81\u51fa\u8868\u660e\uff0cUPR\u7684\u8bf1\u5bfc\u7ed3\u679c\u5177\u6709\u9ad8\u5ea6\u7684\u80cc\u666f\u4f9d\u8d56\u6027\u3002\u8fd9\u610f\u5473\u7740\uff0c\u5373\u4f7f\u662f\u7ecf\u5178\u7684UPR\u8bf1\u5bfc\u5242\uff0c\u5176\u5728\u7279\u5b9a\u7ec6\u80de\u73af\u5883\uff08\u4f8b\u5982\uff0c\u5b58\u5728\u75c5\u6bd2\u611f\u67d3\u3001\u7279\u5b9a\u7684\u7ec6\u80de\u7cfb\u7279\u6027\u6216\u5176\u4ed6\u5e76\u53d1\u523a\u6fc0\uff09\u4e2d\u7684\u4f5c\u7528\u4e5f\u53ef\u80fd\u663e\u8457\u6539\u53d8UPR\u7684\u54cd\u5e94\u3002\u56e0\u6b64\uff0c\u7814\u7a76\u4eba\u5458\u4e0d\u5e94\u5047\u8bbeTG\u548cTM\u4f1a\u4ea7\u751f\u76f8\u540c\u7684UPR\u7ed3\u679c\uff0c\u5c24\u5176\u662f\u5728\u7814\u7a76\u764c\u75c7\u6216\u75c5\u6bd2\u611f\u67d3\u7b49\u590d\u6742\u751f\u7269\u7cfb\u7edf\u65f6\uff0c\u5e94\u9a8c\u8bc1\u5176\u6240\u9009\u6a21\u578b\u4e2d\u7279\u5b9aUPR\u901a\u8def\u7684\u6fc0\u6d3b\u60c5\u51b5\u3002<\/p>\n\n\n\n

5.2 \u5b9e\u9a8c\u8bbe\u8ba1\u8003\u91cf<\/h3>\n\n\n\n

\u9274\u4e8e\u9ad8\u6d53\u5ea6\u6216\u957f\u65f6\u95f4\u66b4\u9732\u53ef\u80fd\u5bfc\u81f4\u7ec6\u80de\u6bd2\u6027 23<\/sup>\uff0c\u7814\u7a76\u4eba\u5458\u5e94\u4ed4\u7ec6\u6ef4\u5b9a\u6d53\u5ea6\u548c\u6301\u7eed\u65f6\u95f4\uff0c\u4ee5\u5b9e\u73b0\u6240\u9700\u7684UPR\u6fc0\u6d3b\uff08\u4f8b\u5982\uff0c\u9002\u5e94\u6027UPR\u6216\u4fc3\u51cb\u4ea1UPR\uff09\uff0c\u540c\u65f6\u6700\u5927\u7a0b\u5ea6\u5730\u51cf\u5c11\u4e0d\u5fc5\u8981\u7684\u7ec6\u80de\u6b7b\u4ea1\u3002\u5bf9\u4e8eK562\u7ec6\u80de\uff0c\u57fa\u4e8e\u5bf9\u5176\u4ed6\u4eba\u7c7b\u7ec6\u80de\u7cfb\u548c\u6709\u9650K562\u6570\u636e\u7684\u63a8\u65ad\uff0c\u5efa\u8bae\u7684\u8d77\u59cb\u8303\u56f4\u662f<\/p>\n\n\n\n

500 nM\u81f31 \u00b5M\u7684\u786b\u666e\u897f\u52a0<\/strong> \u548c 4-5 \u00b5g\/mL\u7684\u8863\u9709\u7d20<\/strong>\uff0c\u66b4\u9732\u6301\u7eed\u65f6\u95f4\u4e3a 8-12\u5c0f\u65f6<\/strong>\u3002\u7136\u800c\uff0c\u5f3a\u70c8\u5efa\u8bae\u8fdb\u884c\u7ecf\u9a8c\u6027\u4f18\u5316\u3002<\/p>\n\n\n\n

\u6570\u636e\u4e2d\u5448\u73b0\u7684TG\u548cTM\u5728\u4e0d\u540c\u4eba\u7c7b\u7ec6\u80de\u7cfb\u4e2d\u6709\u6548\u6d53\u5ea6\u548c\u6301\u7eed\u65f6\u95f4\u7684\u8303\u56f4\uff0c\u4ee5\u53ca\u5728\u7279\u5b9a\u80cc\u666f\u4e0b\uff08\u5982Huh-7\u7ec6\u80de\uff09\u51fa\u73b0\u7684\u53d8\u5f02\u751a\u81f3\u77db\u76fe\u7ed3\u679c 4<\/sup>\uff0c\u8868\u660e\u201c\u4e00\u5200\u5207\u201d\u7684\u65b9\u6cd5\u662f\u4e0d\u8db3\u7684\u3002\u5bf9\u4e8eK562\u7ec6\u80de\uff0c\u7531\u4e8e\u76f4\u63a5\u7cbe\u786e\u6570\u636e\u6709\u9650\uff0c\u7814\u7a76\u4eba\u5458\u5fc5\u987b\u8fdb\u884c\u7ec6\u81f4\u7684\u5242\u91cf-\u53cd\u5e94\u548c\u65f6\u95f4\u8fdb\u7a0b\u5b9e\u9a8c\u3002\u8fd9\u79cd\u7cbe\u786e\u6027\u81f3\u5173\u91cd\u8981\uff0c\u56e0\u4e3aUPR\u662f\u53cc\u76f8\u7684\uff08\u9002\u5e94\u6027\u4e0e\u51cb\u4ea1\u6027\uff09 4<\/sup>\uff0c\u5e76\u4e14\u5b9e\u9a8c\u76ee\u7684\uff08\u4f8b\u5982\uff0c\u7814\u7a76\u9002\u5e94\u6027\u53cd\u5e94\u3001\u8bf1\u5bfc\u7ec6\u80de\u6b7b\u4ea1\u6216\u63a2\u7a76\u836f\u7269\u534f\u540c\u4f5c\u7528\uff09\u5c06\u51b3\u5b9a\u6700\u4f73\u53c2\u6570\u3002\u56e0\u6b64\uff0c\u5b9e\u9a8c\u8bbe\u8ba1\u5e94\u662f\u8fed\u4ee3\u7684\uff0c\u4ece\u5efa\u8bae\u7684\u8303\u56f4\u5f00\u59cb\uff0c\u5e76\u8fdb\u884c\u5fae\u8c03\u4ee5\u5728K562\u7ec6\u80de\u4e2d\u5b9e\u73b0\u6240\u9700\u7684UPR\u72b6\u6001\u3002<\/p>\n\n\n\n

6. \u8bc4\u4f30XBP1\u526a\u63a5\u7684\u65b9\u6cd5\u5b66\u8003\u91cf<\/h2>\n\n\n\n

6.1 \u5e38\u7528\u6280\u672f<\/h3>\n\n\n\n

\u8bc4\u4f30XBP1\u526a\u63a5\u7684\u65b9\u6cd5\u9009\u62e9\u5bf9\u6240\u83b7\u5f97\u6570\u636e\u7684\u7cbe\u786e\u6027\u548c\u7c7b\u578b\u5177\u6709\u76f4\u63a5\u5f71\u54cd\u3002<\/p>\n\n\n\n

    \n
  • \u9006\u8f6c\u5f55\u805a\u5408\u9176\u94fe\u53cd\u5e94 (RT-PCR) \/ \u5b9a\u91cf\u9006\u8f6c\u5f55\u805a\u5408\u9176\u94fe\u53cd\u5e94 (qRT-PCR):<\/strong> \u8fd9\u662f\u68c0\u6d4bXBP1 mRNA\u526a\u63a5\u6700\u5e38\u7528\u7684\u65b9\u6cd5 3<\/sup>\u3002\u8be5\u65b9\u6cd5\u4f9d\u8d56\u4e8e26\u4e2a\u6838\u82f7\u9178\u5185\u542b\u5b50\u7684\u5207\u9664\uff0c\u5bfc\u81f4XBP1s\u7684\u6269\u589e\u5b50\u6bd4XBP1u\u5c0f\uff0c\u53ef\u901a\u8fc7\u51dd\u80f6\u7535\u6cf3\u68c0\u6d4b 3<\/sup>\u3002\u4f7f\u7528\u7279\u5f02\u6027\u5f15\u7269\u533a\u5206\u526a\u63a5\u548c\u672a\u526a\u63a5\u5f62\u5f0f\uff08\u4f8b\u5982\uff0c19<\/sup>\u4e2d\u63d0\u53ca\u7684XBP1spliceF\/R\u5f15\u7269\uff09\u3002qRT-PCR\u5141\u8bb8\u5bf9sXBP1 mRNA\u6c34\u5e73\u8fdb\u884c\u5b9a\u91cf\u6d4b\u91cf 13<\/sup>\u3002<\/li>\n\n\n\n
  • XBP1\u526a\u63a5\u62a5\u544a\u57fa\u56e0\u7ec6\u80de\u7cfb:<\/strong> \u8fd9\u4e9b\u5de5\u7a0b\u5316\u7ec6\u80de\u7cfb\u5305\u542b\u4e00\u4e2a\u62a5\u544a\u57fa\u56e0\uff08\u4f8b\u5982\uff0c\u7eb3\u7c73\u8367\u5149\u7d20\u9176\uff09\uff0c\u5176\u8868\u8fbe\u4f9d\u8d56\u4e8eIRE1\u4ecb\u5bfc\u7684XBP1\u526a\u63a5 7<\/sup>\u3002\u8fd9\u4f7f\u5f97\u80fd\u591f\u5b9e\u65f6\u76d1\u6d4bIRE1\u5185\u5207\u6838\u9178\u9176\u6d3b\u6027\uff0c\u5e76\u8bc6\u522b\u8c03\u8282\u5185\u8d28\u7f51\u5e94\u6fc0\u7684\u5206\u5b50 7<\/sup>\u3002\u6b64\u7c7b\u62a5\u544a\u7cfb\u7edf\u63d0\u4f9b\u4e86\u4e00\u79cd\u53ef\u9760\u4e14\u51c6\u786e\u7684\u65b9\u6cd5\u6765\u7814\u7a76IRE1\u7684\u8c03\u63a7\u4ee5\u53ca\u8bc6\u522b\u5185\u8d28\u7f51\u5e94\u6fc0\u8c03\u8282\u5206\u5b50\u3002<\/li>\n\n\n\n
  • Western Blotting:<\/strong> \u867d\u7136\u4e0d\u76f4\u63a5\u6d4b\u91cfmRNA\u526a\u63a5\uff0c\u4f46Western blot\u53ef\u4ee5\u68c0\u6d4bXBP1s\u7684\u86cb\u767d\u8d28\u4ea7\u7269\uff0c\u7531\u4e8e\u79fb\u7801\uff0c\u5176\u5206\u5b50\u91cf\u4e0d\u540c\uff0c\u6216\u68c0\u6d4b\u7531XBP1s\u8bf1\u5bfc\u7684\u4e0b\u6e38UPR\u9776\u57fa\u56e0\uff08\u4f8b\u5982\uff0cBiP\u3001CHOP\uff0916<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n

    \u65b9\u6cd5\u9009\u62e9\u5f71\u54cd\u6570\u636e\u7684\u7cbe\u786e\u6027\u548c\u7c7b\u578b\u3002RT-PCR\u662f\u68c0\u6d4b\u526a\u63a5\u4e8b\u4ef6\u672c\u8eab\u7684\u6807\u51c6\u65b9\u6cd5 3<\/sup>\uff0c\u800cqRT-PCR\u5219\u91cf\u5316\u526a\u63a5XBP1 mRNA\u7684\u6c34\u5e73 13<\/sup>\u3002\u62a5\u544a\u57fa\u56e0\u7ec6\u80de\u7cfb 7<\/sup> \u63d0\u4f9b\u9ad8\u901a\u91cf\u7b5b\u9009\u548c\u5b9e\u65f6\u52a8\u529b\u5b66\uff0c\u4f46\u4f9d\u8d56\u4e8e\u5916\u6e90\u6784\u5efa\u4f53\u3002Western blotting 16<\/sup> \u8bc4\u4f30\u86cb\u767d\u8d28\u4ea7\u7269\u548c\u4e0b\u6e38\u6548\u5e94\uff0c\u8fd9\u53ef\u80fd\u6bd4mRNA\u526a\u63a5\u6709\u5ef6\u8fdf\u3002\u67d0\u4e9bRT-PCR\u65b9\u6cd5\u7684\u201c\u534a\u5b9a\u91cf\u201d\u6027\u8d28 22<\/sup> \u610f\u5473\u7740\u7cbe\u786e\u7684\u500d\u6570\u53d8\u5316\u6700\u597d\u901a\u8fc7qRT-PCR\u8fdb\u884c\u8bc4\u4f30\u3002\u56e0\u6b64\uff0c\u7814\u7a76\u4eba\u5458\u5e94\u6839\u636e\u5176\u5177\u4f53\u7684\u5b9e\u9a8c\u95ee\u9898\uff08\u4f8b\u5982\uff0c\u5b9a\u6027\u68c0\u6d4b\u4e0e\u5b9a\u91cf\u52a8\u529b\u5b66\uff09\u9009\u62e9\u5408\u9002\u7684\u65b9\u6cd5\uff0c\u5e76\u4e86\u89e3\u5176\u5c40\u9650\u6027\u4ee5\u53ca\u5b83\u5b9e\u9645\u6d4b\u91cf\u7684\u662fUPR\u6fc0\u6d3b\u7684\u54ea\u4e2a\u65b9\u9762\u3002<\/p>\n\n\n\n

    7. \u7ed3\u8bba\u4e0e\u672a\u6765\u7814\u7a76\u65b9\u5411<\/h2>\n\n\n\n

    7.1 K562\u7ec6\u80deUPR\u8bf1\u5bfc\u7684\u6700\u4f73\u6761\u4ef6\u603b\u7ed3<\/h3>\n\n\n\n

    \u57fa\u4e8e\u73b0\u6709\u6587\u732e\uff0c\u5c3d\u7ba1\u76f4\u63a5\u62a5\u544a\u7684\u7528\u4e8eK562\u7ec6\u80deXBP1\u526a\u63a5\u7684TG\u548cTM\u7cbe\u786e\u6d53\u5ea6\u6709\u9650\uff0c\u4f46\u5728\u7528\u8fd9\u4e24\u79cd\u836f\u7269\u5904\u7406K562\u7ec6\u80de8\u5c0f\u65f6\u540e\uff0c\u89c2\u5bdf\u5230\u4e86\u5f3a\u70c8\u7684UPR\u6fc0\u6d3b\uff0c\u5305\u62ecXBP1\u526a\u63a5 9<\/sup>\u3002\u8863\u9709\u7d20\u4e5f\u88ab\u8bc1\u5b9e\u80fd\u8bf1\u5bfcK562\u7ec6\u80de\u7684\u526a\u63a5 22<\/sup>\u3002<\/p>\n\n\n\n

    \u4ece\u5176\u4ed6\u4eba\u7c7b\u7ec6\u80de\u7cfb\u63a8\u65ad\uff0c\u786b\u666e\u897f\u52a0\u7684\u6709\u6548\u6d53\u5ea6\u8303\u56f4\u4e3a 500 nM\u81f310 \u00b5M<\/strong> 3<\/sup>\uff0c\u5176\u4e2d500 nM\u5728\u6c38\u751f\u5316B\u7ec6\u80de\u4e2d8\u5c0f\u65f6\u5185\u663e\u793a\u51faXBP1\u526a\u63a5 3<\/sup>\u3002\u5bf9\u4e8e\u8863\u9709\u7d20\uff0c<\/p>\n\n\n\n

    4-5 \u00b5g\/mL<\/strong> \u7684\u6d53\u5ea6\u5728\u5176\u4ed6\u4eba\u7c7b\u7ec6\u80de\u7cfb\uff08\u6c38\u751f\u5316B\u7ec6\u80de\u3001Calu-3\u3001HeLa\uff09\u4e2d\u6301\u7eed 8-12\u5c0f\u65f6<\/strong> \u53ef\u6709\u6548\u8bf1\u5bfcXBP1\u526a\u63a5 3<\/sup>\u3002\u56e0\u6b64\uff0c\u5efa\u8bae\u5728K562\u7ec6\u80de\u4e2d\u8bf1\u5bfcXBP1\u526a\u63a5\u7684\u8d77\u59cb\u70b9\u4e3a<\/p>\n\n\n\n

    500 nM\u81f31 \u00b5M\u7684\u786b\u666e\u897f\u52a0<\/strong> \u548c 4-5 \u00b5g\/mL\u7684\u8863\u9709\u7d20<\/strong>\uff0c\u66b4\u9732\u6301\u7eed\u65f6\u95f4\u4e3a 8-12\u5c0f\u65f6<\/strong>\u3002<\/p>\n\n\n\n

    7.2 \u5bf9\u767d\u8840\u75c5\u7814\u7a76\u548c\u6cbb\u7597\u7b56\u7565\u7684\u66f4\u5e7f\u6cdb\u5f71\u54cd<\/h3>\n\n\n\n

    UPR\u5728\u767d\u8840\u75c5\u4e2d\u626e\u6f14\u7740\u590d\u6742\u7684\u89d2\u8272\uff0c\u65e2\u662f\u4fc3\u751f\u5b58\u673a\u5236\uff0c\u4e5f\u662f\u8bf1\u5bfc\u7ec6\u80de\u6b7b\u4ea1\u7684\u6f5c\u5728\u9776\u70b9 5<\/sup>\u3002\u5728K562\u7ec6\u80de\u4e2d\u7cbe\u786e\u63a7\u5236UPR\u8bf1\u5bfc\uff08\u4ee5XBP1\u526a\u63a5\u4e3a\u6307\u6807\uff09\u5bf9\u4e8e\u5256\u6790\u8fd9\u4e9b\u53cc\u91cd\u4f5c\u7528\u4ee5\u53ca\u5f00\u53d1\u9488\u5bf9\u9ad3\u7cfb\u767d\u8840\u75c5\u7684\u9776\u5411\u6cbb\u7597\u7b56\u7565\u81f3\u5173\u91cd\u8981 8<\/sup>\u3002<\/p>\n\n\n\n

    \u5c3d\u7ba1\u672c\u62a5\u544a\u6c47\u7f16\u4e86\u73b0\u6709\u6570\u636e\uff0c\u4f46K562\u7ec6\u80de\u7684\u7cbe\u786e\u6700\u4f73\u6d53\u5ea6\u548c\u6301\u7eed\u65f6\u95f4\u4ecd\u9700\u8fdb\u4e00\u6b65\u63a8\u65ad\u3002\u8fd9\u51f8\u663e\u4e86\u8f6c\u5316\u7814\u7a76\u4e2d\u7684\u4e00\u4e2a\u5173\u952e\u7a7a\u767d\u3002\u9274\u4e8eUPR\u53ef\u4ee5\u662f\u4fc3\u751f\u5b58\u6216\u4fc3\u51cb\u4ea1\u7684 5<\/sup>\uff0c\u5e76\u4e14\u7279\u5b9a\u7ec6\u80de\u80cc\u666f\uff08\u4f8b\u5982K562\u7684\u767d\u8840\u75c5\u8d77\u6e90\uff09\u4f1a\u5f71\u54cdUPR\u7ed3\u679c 4<\/sup>\uff0c\u56e0\u6b64\u5bf9K562\u7ec6\u80de\u8fdb\u884c\u7ecf\u9a8c\u6027\u4f18\u5316\u4e0d\u4ec5\u662f\u826f\u597d\u5b9e\u8df5\uff0c\u66f4\u662f\u5fc5\u8981\u4e4b\u4e3e\u3002\u8fd9\u786e\u4fdd\u4e86\u6240\u8bf1\u5bfc\u7684UPR\u72b6\u6001\u4e0e\u7814\u7a76\u76ee\u6807\u76f8\u7b26\uff0c\u65e0\u8bba\u662f\u7814\u7a76\u9002\u5e94\u6027\u673a\u5236\u3001\u8010\u836f\u6027\u8fd8\u662f\u8bf1\u5bfc\u7ec6\u80de\u6b7b\u4ea1\u4ee5\u8fbe\u5230\u6cbb\u7597\u76ee\u7684\u3002\u8fd9\u5f3a\u8c03\u4e86\u79d1\u5b66\u63a2\u7a76\u7684\u8fed\u4ee3\u6027\u8d28\u4ee5\u53ca\u5728\u7279\u5b9a\u6a21\u578b\u7cfb\u7edf\u4e2d\u9a8c\u8bc1\u53d1\u73b0\u5bf9\u4e8e\u8f6c\u5316\u5f71\u54cd\u7684\u91cd\u8981\u6027\u3002\u672a\u6765\u7684\u7814\u7a76\u5e94\u4fa7\u91cd\u4e8e\u901a\u8fc7\u8be6\u7ec6\u7684\u5242\u91cf-\u53cd\u5e94\u548c\u65f6\u95f4\u8fdb\u7a0b\u7814\u7a76\uff0c\u4e13\u95e8\u9488\u5bf9K562\u7ec6\u80de\u4f18\u5316\u8fd9\u4e9b\u6761\u4ef6\uff0c\u5c24\u5176\u8981\u8003\u8651TG\u548cTM\u5bf9UPR\u901a\u8def\u53ef\u80fd\u4ea7\u751f\u7684\u5dee\u5f02\u6027\u8c03\u8282\u4ee5\u53ca\u4ece\u9002\u5e94\u6027UPR\u5411\u7ec6\u80de\u51cb\u4ea1\u7684\u8f6c\u53d8\u3002<\/p>\n\n\n\n

    <\/p>\n\n\n\n

    \"\"
    0.5 \u03bcg\/mL TM\u5904\u7406K562 6h\uff0c2h\u5b8c\u5168Xbp1s\uff0c\u4f466h\u53c8\u5168\u90e8\u6062\u590dXbp1u\u4e86<\/figcaption><\/figure>\n\n\n\n
    \"\"
    Tg, Tm and H2O2 individually in 5 \u00b5g\/l, 0.1 \u00b5M and 3 \u00b5M respectively. cells were subjected to treatments
    for 8 hours<\/strong>.<\/figcaption><\/figure>\n\n\n\n

    \u8fd9\u91cc0.1 \u03bcM TM\u6362\u7b97\u662f0.84 \u03bcg\/mL<\/p>\n\n\n\n

    <\/p>\n\n\n\n

    \"\"
    Total period of exposure was eight hours.<\/strong> The utilized concentrations of H2<\/sub>O2<\/sub>, Tg, and Tm were 3 \u00b5M, 5 \u03bcg\/L, and 1 \u00b5M, respectively.<\/figcaption><\/figure>\n\n\n\n

    \u4e0a\u9762\u4e24\u5f20\u56fe\u7684\u6587\u732e\u90fd\u7528\u4e865\u03bcg\/L \u7684 Thapsigargin\uff0c \u7ea6\u7b49\u4e8e0.0076\u03bcM<\/strong>\uff1b\u4f46\u662fTM\u5206\u522b\u7528\u4e860.1\u03bcM\u548c1\u03bcM<\/p>\n\n\n\n

    \u6211\u8981\u505a\u4e2a\u9884\u5b9e\u9a8c\u6478\u7d22\u6d53\u5ea6\u548c\u65f6\u95f4\uff0c\u5148\u5c1d\u8bd5\uff1a<\/p>\n\n\n\n

      \n
    • TG\u7ec4<\/strong>: 0.01, 0.1, 1 \u00b5M (\u5904\u7406\u00a06\u5c0f\u65f6<\/strong>)\u3002<\/li>\n\n\n\n
    • TM\u7ec4<\/strong>: 0.01, 0.1, 1 \u03bcM (\u5904\u7406\u00a06\u5c0f\u65f6<\/strong>)\u3002<\/li>\n<\/ul>\n\n\n\n

      <\/p>\n\n\n\n

      K562\u7ec6\u80deER Stress\u8bf1\u5bfc (12\u5b54\u677f, 1mL\u4f53\u7cfb)<\/strong><\/h3>\n\n\n\n
      \n\n\n\n

      \u4e00\u3001\u51c6\u5907<\/strong><\/h4>\n\n\n\n
        \n
      1. \u7ec6\u80de<\/strong>: K562\u7ec6\u80de\uff0c\u5bf9\u6570\u751f\u957f\u671f\u3002<\/li>\n\n\n\n
      2. \u6bcd\u6db2<\/strong>: 1 mM TG (in DMSO), 1 mM TM (in DMSO)\u3002<\/li>\n\n\n\n
      3. \u8017\u6750<\/strong>: 12\u5b54\u677f\u3002<\/li>\n<\/ol>\n\n\n\n

        \u4e8c\u3001\u6b65\u9aa4<\/strong><\/h4>\n\n\n\n
          \n
        1. \u94fa\u677f<\/strong>:\n
            \n
          • \u6bcf\u5b54\u94fa\u00a00.5 x 10\u2076<\/strong>\u00a0\u4e2a\u7ec6\u80de\uff0c\u7528\u57f9\u517b\u57fa\u8865\u8db3\u81f3\u00a01 mL<\/strong>\u3002<\/li>\n<\/ul>\n<\/li>\n\n\n\n
          • \u836f\u7269\u7a00\u91ca (\u7528DMSO)<\/strong>:\n
              \n
            • 100 \u00b5M\u5de5\u4f5c\u6db2<\/strong>: \u53d6\u00a02 \u00b5L<\/strong>\u00a0\u76841 mM\u6bcd\u6db2 +\u00a018 \u00b5L<\/strong>\u00a0DMSO\u3002<\/li>\n\n\n\n
            • 10 \u00b5M\u5de5\u4f5c\u6db2<\/strong>: \u53d6\u00a02 \u00b5L<\/strong>\u00a0\u7684100 \u00b5M\u5de5\u4f5c\u6db2 +\u00a018 \u00b5L<\/strong>\u00a0DMSO\u3002<\/li>\n<\/ul>\n<\/li>\n\n\n\n
            • \u52a0\u836f (\u6bcf\u5b54\u52a0\u5165 1 \u00b5L)<\/strong>:<\/li>\n<\/ol>\n\n\n\n
              \u7ec4\u522b<\/th>\u76ee\u6807\u7ec8\u6d53\u5ea6<\/th>\u52a0\u5165\u6db2\u4f53 (1 \u00b5L)<\/th><\/tr><\/thead>
              1. \u5bf9\u7167<\/strong><\/td>0 (DMSO)<\/td>DMSO<\/td><\/tr>
              2. TG-\u4f4e<\/strong><\/td>0.01 \u00b5M<\/td>10 \u00b5M TG<\/td><\/tr>
              3. TG-\u4e2d<\/strong><\/td>0.1 \u00b5M<\/td>100 \u00b5M TG<\/td><\/tr>
              4. TG-\u9ad8<\/strong><\/td>1 \u00b5M<\/td>1 mM TG<\/td><\/tr>
              5. TM-\u4f4e<\/strong><\/td>0.01 \u00b5M<\/td>10 \u00b5M TM<\/td><\/tr>
              6. TM-\u4e2d<\/strong><\/td>0.1 \u00b5M<\/td>100 \u00b5M TM<\/td><\/tr>
              7. TM-\u9ad8<\/strong><\/td>1 \u00b5M<\/td>1 mM TM<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
                \n
              1. \u57f9\u517b<\/strong>:\n
                  \n
                • \u6df7\u5300\uff0c\u653e\u5165\u57f9\u517b\u7bb1\u5b75\u80b2\u00a06 \u5c0f\u65f6<\/strong>\u3002<\/li>\n<\/ul>\n<\/li>\n\n\n\n
                • \u6536\u83b7<\/strong>:\n
                    \n
                  • \u5439\u6253\u7ec6\u80de\uff0c\u79bb\u5fc3\uff0cPBS\u6d17\u6da4\uff0c\u63d0\u53d6RNA\u6216\u86cb\u767d\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

                    \u8fd9\u7bc7\u7b14\u8bb0\u7cfb\u7edf\u68b3\u7406\u4e86\u5728K562\u4eba\u9ad3\u7cfb\u767d\u8840\u75c5\u7ec6\u80de\u7cfb\u4e2d\uff0c\u4f7f\u7528\u786b\u666e\u897f\u52a0\uff08TG\uff09\u548c\u8863\u9709\u7d20\uff08TM\uff09\u8bf1\u5bfc\u672a\u6298\u53e0\u86cb\u767d\u53cd\u5e94\uff08UPR\uff09\u7684\u5173\u952e\u5b9e\u9a8c\u53c2\u6570\u3002\u62a5\u544a\u805a\u7126\u4e8eXBP1\u526a\u63a5\u8fd9\u4e00\u6838\u5fc3\u6307\u6807\uff0c\u901a\u8fc7\u6574\u5408\u73b0\u6709\u6587\u732e\u6570\u636e\uff0c\u4e3a\u7814\u7a76\u8005\u63d0\u4f9b\u4e86\u57fa\u4e8e\u5176\u4ed6\u4eba\u7c7b\u7ec6\u80de\u7cfb\u63a8\u65ad\u7684\u6d53\u5ea6\u4e0e\u6301\u7eed\u65f6\u95f4\u53c2\u8003\u8303\u56f4\uff0c\u5e76\u5f3a\u8c03\u4e86\u8fdb\u884c\u7ecf\u9a8c\u6027\u4f18\u5316\u7684\u91cd\u8981\u6027\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[32],"class_list":["post-10494","post","type-post","status-publish","format-standard","hentry","category-note","tag-deep-research"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/10494","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10494"}],"version-history":[{"count":12,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/10494\/revisions"}],"predecessor-version":[{"id":13843,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/10494\/revisions\/13843"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10494"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10494"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10494"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}