{"id":10691,"date":"2025-07-15T02:49:47","date_gmt":"2025-07-14T18:49:47","guid":{"rendered":"https:\/\/fanyiming.life\/?p=10691"},"modified":"2025-12-21T01:03:25","modified_gmt":"2025-12-20T17:03:25","slug":"%e8%b6%85%e8%b6%8a%e7%bb%8f%e5%85%b8%ef%bc%9a%e5%af%b9rna%e8%bf%9e%e6%8e%a5%e9%85%b6rtcb%e6%89%a9%e5%b1%95%e5%ba%95%e7%89%a9%e8%b0%b1%e5%8f%8a%e5%8a%9f%e8%83%bd%e5%ba%93%e7%9a%84%e8%af%a6%e5%b0%bd","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=10691","title":{"rendered":"\u8d85\u8d8a\u7ecf\u5178\uff1a\u5bf9RNA\u8fde\u63a5\u9176RTCB\u6269\u5c55\u5e95\u7269\u8c31\u53ca\u529f\u80fd\u5e93\u7684\u8be6\u5c3d\u5206\u6790"},"content":{"rendered":"\n
\n

By Google Gemini 2.5 Pro DeepResearch<\/p>\n<\/blockquote>\n\n\n\n

\u7b2c\u4e00\u8282\uff1a\u5df2\u786e\u7acb\u7684\u8303\u5f0f\uff1aRTCB\u5728tRNA\u548cXBP1 mRNA\u975e\u526a\u63a5\u4f53\u4f9d\u8d56\u6027\u526a\u63a5\u4e2d\u7684\u6838\u5fc3\u529f\u80fd<\/h2>\n\n\n\n

RNA\u8fde\u63a5\u9176RTCB\uff08RNA 2′,3′-Cyclic Phosphate and 5′-OH Ligase\uff09\u662f\u4e00\u79cd\u5728\u751f\u547d\u8fdb\u5316\u4e2d\u9ad8\u5ea6\u4fdd\u5b88\u7684\u9176\uff0c\u5176\u72ec\u7279\u7684\u50ac\u5316\u673a\u5236\u548c\u5728\u5173\u952e\u7ec6\u80de\u8fc7\u7a0b\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u4f5c\u7528\uff0c\u4f7f\u5176\u6210\u4e3aRNA\u751f\u7269\u5b66\u7814\u7a76\u7684\u7126\u70b9\u3002\u5c3d\u7ba1\u5176\u529f\u80fd\u591a\u6837\u6027\u65e5\u76ca\u663e\u73b0\uff0c\u4f46RTCB\u7684\u7ecf\u5178\u89d2\u8272\u4e3b\u8981\u96c6\u4e2d\u5728\u4e24\u4e2a\u4e0d\u4f9d\u8d56\u4e8e\u5927\u578b\u526a\u63a5\u4f53\u590d\u5408\u7269\u7684RNA\u526a\u63a5\u4e8b\u4ef6\u4e2d\uff1atRNA\u524d\u4f53\u7684\u6210\u719f\u548c\u5728\u672a\u6298\u53e0\u86cb\u767d\u53cd\u5e94\uff08Unfolded Protein Response, UPR\uff09\u4e2dXBP1 mRNA\u7684\u975e\u5e38\u89c4\u526a\u63a5\u3002\u7406\u89e3\u8fd9\u4e9b\u6838\u5fc3\u529f\u80fd\uff0c\u5bf9\u4e8e\u63a2\u7d22\u5176\u66f4\u5e7f\u6cdb\u7684\u5e95\u7269\u8303\u56f4\u548c\u751f\u7269\u5b66\u610f\u4e49\u81f3\u5173\u91cd\u8981\u3002<\/p>\n\n\n\n

1.1 \u5b9a\u4e49RTCB\uff1a\u4e00\u79cd\u72ec\u7279\u7684\u975e\u7ecf\u5178RNA\u8fde\u63a5\u9176<\/h3>\n\n\n\n

RTCB\u5728\u53e4\u83cc\u3001\u7ec6\u83cc\u548c\u540e\u751f\u52a8\u7269\u4e2d\u5e7f\u6cdb\u5b58\u5728\uff0c\u4f46\u5728\u5927\u591a\u6570\u771f\u83cc\u548c\u690d\u7269\u4e2d\u5374\u663e\u8457\u7f3a\u5931\uff0c\u8fd9\u6697\u793a\u4e86\u5176\u5728\u7279\u5b9a\u751f\u547d\u57df\u4e2d\u626e\u6f14\u7740\u72ec\u7279\u7684\u8fdb\u5316\u89d2\u8272 1<\/sup>\u3002\u5728\u4eba\u7c7b\u7ec6\u80de\u4e2d\uff0cRTCB\u662f\u8fc4\u4eca\u4e3a\u6b62\u552f\u4e00\u88ab\u9274\u5b9a\u51fa\u76843′-5′ RNA\u8fde\u63a5\u9176 4<\/sup>\u3002\u5176\u6700\u6839\u672c\u7684\u7279\u5f81\u5728\u4e8e\u5176\u72ec\u7279\u7684\u5e95\u7269\u504f\u597d\u6027\uff1aRTCB\u50ac\u5316\u5e26\u67093’\u78f7\u9178\u57fa\u56e2\uff08<\/p>\n\n\n\n

3\u2032-P\uff09\u548c5’\u7f9f\u57fa\uff085\u2032-OH\uff09\u7684RNA\u65ad\u88c2\u672b\u7aef\u7684\u8fde\u63a5\uff0c\u5f62\u6210\u4e00\u4e2a\u6807\u51c6\u76843′,5′-\u78f7\u9178\u4e8c\u916f\u952e 1<\/sup>\u3002\u8fd9\u4e00\u7279\u6027\u4f7f\u5176\u4e0e\u4f9d\u8d565’\u78f7\u9178\u548c3’\u7f9f\u57fa\u672b\u7aef\u7684\u7ecf\u5178RNA\u8fde\u63a5\u9176\uff08\u5982T4 RNA ligase\uff09\u622a\u7136\u4e0d\u540c\u3002<\/p>\n\n\n\n

\u66f4\u4e3a\u91cd\u8981\u7684\u662f\uff0cRTCB\u5177\u5907\u5904\u7406\u66f4\u4e3a\u590d\u6742\u7684RNA\u672b\u7aef\u7684\u80fd\u529b\u3002\u5f53\u5e95\u7269\u672b\u7aef\u4e3a2′,3′-\u73af\u78f7\u9178\uff082\u2032,3\u2032-cyclic phosphate\uff09\u65f6\uff0cRTCB\u80fd\u591f\u9996\u5148\u5c06\u5176\u6c34\u89e3\u4e3a3′-\u78f7\u9178\uff0c\u7136\u540e\u518d\u8fdb\u884c\u8fde\u63a5\u53cd\u5e94 1<\/sup>\u3002\u8fd9\u4e00\u201c\u4fee\u590d\u5e76\u5c01\u95ed\u201d\uff08healing-and-sealing\uff09\u7684\u53cc\u91cd\u529f\u80fd\uff0c\u4f7f\u5f97RTCB\u80fd\u591f\u76f4\u63a5\u5904\u7406\u7531\u7279\u5b9a\u6838\u9178\u5185\u5207\u9176\uff08\u5982IRE1\u548ctRNA\u526a\u63a5\u5185\u5207\u9176\uff09\u5207\u5272\u4ea7\u751f\u7684RNA\u7247\u6bb5\uff0c\u800c\u65e0\u9700\u5176\u4ed6\u8f85\u52a9\u9176\u5bf9\u672b\u7aef\u8fdb\u884c\u4fee\u9970 1<\/sup>\u3002\u8fd9\u4e00\u53d1\u73b0\u89e3\u51b3\u4e86\u65e9\u671f\u5173\u4e8eRTCB\u50ac\u5316\u673a\u5236\u7684\u4e89\u8bae\uff0c\u5e76\u786e\u7acb\u4e86\u5176\u5728RNA\u4fee\u590d\u548c\u52a0\u5de5\u901a\u8def\u4e2d\u7684\u6838\u5fc3\u5730\u4f4d 1<\/sup>\u3002<\/p>\n\n\n\n

1.2 \u50ac\u5316\u673a\u5236\uff1a\u4e00\u4e2a\u4f9d\u8d56GTP\u7684\u4e09\u6b65\u53cd\u5e94\u9014\u5f84<\/h3>\n\n\n\n

RTCB\u7684\u50ac\u5316\u6d3b\u6027\u4e25\u683c\u4f9d\u8d56\u4e8eGTP\u548c\u4e8c\u4ef7\u91d1\u5c5e\u79bb\u5b50\uff0c\u5176\u4e2d\u9530\u79bb\u5b50\uff08Mn2+\uff09\u8868\u73b0\u51fa\u6700\u9ad8\u7684\u50ac\u5316\u6548\u7387 1<\/sup>\u3002\u901a\u8fc7\u7cbe\u5bc6\u7684\u7ed3\u6784\u751f\u7269\u5b66\u548c\u751f\u7269\u5316\u5b66\u7814\u7a76\uff0c\u5176\u72ec\u7279\u7684\u50ac\u5316\u673a\u5236\u88ab\u89e3\u6790\u4e3a\u4e00\u4e2a\u5305\u542b\u4e09\u4e2a\u8fde\u7eed\u6838\u82f7\u9170\u8f6c\u79fb\u6b65\u9aa4\u7684\u53cd\u5e94\u901a\u8def 1<\/sup>\u3002<\/p>\n\n\n\n

    \n
  1. \u9176\u7684\u9e1f\u82f7\u9170\u5316\uff08Guanylylation\uff09<\/strong>\uff1a\u53cd\u5e94\u7684\u7b2c\u4e00\u6b65\u662fRTCB\u4e0eGTP\u53d1\u751f\u53cd\u5e94\u3002RTCB\u6d3b\u6027\u4f4d\u70b9\u4e2d\u4e00\u4e2a\u9ad8\u5ea6\u4fdd\u5b88\u7684\u7ec4\u6c28\u9178\u6b8b\u57fa\uff08\u4f8b\u5982\uff0c\u5728\u5927\u80a0\u6746\u83cc\u4e2d\u4e3aHis337\uff0c\u5728\u53e4\u83ccPyrococcus horikoshii<\/em>\u4e2d\u4e3aHis404\uff09\u7684$N^{\\epsilon2}$\u539f\u5b50\u5bf9GTP\u7684$\\alpha$-\u78f7\u9178\u57fa\u56e2\u8fdb\u884c\u4eb2\u6838\u653b\u51fb\uff0c\u5f62\u6210\u4e00\u4e2a\u5171\u4ef7\u7684RTCB-(\u7ec4\u6c28\u9178)-GMP\u4e2d\u95f4\u4f53\uff0c\u540c\u65f6\u91ca\u653e\u7126\u78f7\u9178\uff08PPi\uff091<\/sup>\u3002\u8fd9\u4e00\u6b65\u9aa4\u4e3a\u540e\u7eed\u7684RNA\u6fc0\u6d3b\u63d0\u4f9b\u4e86\u80fd\u91cf\u548c\u8f6c\u79fb\u57fa\u56e2\u3002<\/li>\n\n\n\n
  2. RNA\u7684\u6fc0\u6d3b<\/strong>\uff1a\u63a5\u4e0b\u6765\uff0c\u5171\u4ef7\u8fde\u63a5\u5728\u9176\u4e0a\u7684GMP\u90e8\u5206\u88ab\u8f6c\u79fb\u5230RNA\u5e95\u7269\u76843′-\u78f7\u9178\u57fa\u56e2\u4e0a\u3002\u8fd9\u4e00\u6b65\u5f62\u6210\u4e86\u4e00\u4e2a\u6781\u4e0d\u5bfb\u5e38\u7684\u3001\u9ad8\u80fd\u91cf\u7684RNA-(3′)pp(5′)G\u6d3b\u5316\u4e2d\u95f4\u4f53 1<\/sup>\u3002\u8fd9\u4e2a\u4e2d\u95f4\u4f53\u7684\u5f62\u6210\u662fRTCB\u50ac\u5316\u9014\u5f84\u7684\u5173\u952e\u7279\u5f81\uff0c\u5b83\u5c06\u8fde\u63a5\u53cd\u5e94\u7684\u80fd\u91cf\u50a8\u5b58\u5728RNA\u5e95\u7269\u81ea\u8eab\u4e0a\u3002<\/li>\n\n\n\n
  3. \u8fde\u63a5\u53cd\u5e94<\/strong>\uff1a\u6700\u540e\uff0c\u7b2c\u4e8c\u4e2aRNA\u7247\u6bb5\u76845′-\u7f9f\u57fa\u5bf9\u6d3b\u5316\u76843′-\u672b\u7aef\uff08\u5373RNA-ppG\u4e2d\u95f4\u4f53\u7684\u5185\u90e8\u78f7\u9178\u57fa\u56e2\uff09\u53d1\u8d77\u4eb2\u6838\u653b\u51fb\u3002\u8fd9\u6b21\u653b\u51fb\u5bfc\u81f4\u4e00\u4e2a\u6807\u51c6\u76843′,5′-\u78f7\u9178\u4e8c\u916f\u952e\u7684\u5f62\u6210\uff0c\u4ece\u800c\u5c06\u4e24\u4e2aRNA\u7247\u6bb5\u8fde\u63a5\u8d77\u6765\uff0c\u540c\u65f6\u91ca\u653e\u51faGMP 1<\/sup>\u3002<\/li>\n<\/ol>\n\n\n\n

    \u6574\u4e2a\u50ac\u5316\u5faa\u73af\u5728\u7ed3\u6784\u548c\u5316\u5b66\u5c42\u9762\u4e0a\u90fd\u662f\u72ec\u4e00\u65e0\u4e8c\u7684\uff0c\u8fd9\u4e0d\u4ec5\u5c06RTCB\u4e0e\u6240\u6709\u5df2\u77e5\u7684\u7ecf\u5178\u8fde\u63a5\u9176\u8d85\u5bb6\u65cf\u533a\u5206\u5f00\u6765\uff0c\u4e5f\u63ed\u793a\u4e86\u5176\u4e3a\u9002\u5e94\u7279\u5b9a\u751f\u7269\u5b66\u9700\u6c42\u800c\u6f14\u5316\u51fa\u7684\u9ad8\u5ea6\u7279\u5316\u7684\u529f\u80fd\u8def\u5f84 1<\/sup>\u3002<\/p>\n\n\n\n

    1.3 tRNA\u8fde\u63a5\u9176\u590d\u5408\u7269\uff08tRNA-LC\uff09\uff1aRTCB\u5728\u540e\u751f\u52a8\u7269\u4e2d\u7684\u529f\u80fd\u80cc\u666f<\/h3>\n\n\n\n

    \u5728\u540e\u751f\u52a8\u7269\u4e2d\uff0cRTCB\u5e76\u975e\u72ec\u7acb\u53d1\u6325\u529f\u80fd\uff0c\u800c\u662f\u4f5c\u4e3a\u4e00\u4e2a\u591a\u4e9a\u57fa\u590d\u5408\u7269\u7684\u6838\u5fc3\u50ac\u5316\u5355\u5143\u5b58\u5728\u3002\u8fd9\u4e2a\u590d\u5408\u7269\u88ab\u79f0\u4e3atRNA\u8fde\u63a5\u9176\u590d\u5408\u7269\uff08tRNA Ligase Complex, tRNA-LC\uff09\uff0c\u7531\u4e94\u4e2a\u6838\u5fc3\u86cb\u767d\u7ec4\u6210 5<\/sup>\u3002<\/p>\n\n\n\n

      \n
    • RTCB<\/strong>\uff1a\u4f5c\u4e3a\u590d\u5408\u7269\u7684\u50ac\u5316\u6838\u5fc3\uff0c\u8d1f\u8d23\u6267\u884cRNA\u8fde\u63a5\u53cd\u5e94\u3002<\/li>\n\n\n\n
    • DDX1<\/strong>\uff1a\u4e00\u79cd\u4f9d\u8d56ATP\u7684DEAD-box RNA\u89e3\u65cb\u9176\u3002\u7814\u7a76\u8868\u660e\uff0cDDX1\u7684\u89e3\u65cb\u9176\u6d3b\u6027\u5bf9\u4e8eRTCB\u7684\u5b8c\u5168\u9e1f\u82f7\u9170\u5316\u662f\u5fc5\u9700\u7684\uff0c\u8fd9\u6697\u793aDDX1\u53ef\u80fd\u8d1f\u8d23\u89e3\u5f00RNA\u5e95\u7269\u7684\u4e8c\u7ea7\u7ed3\u6784\uff0c\u6216\u8005\u901a\u8fc7\u91cd\u5851\u590d\u5408\u7269\u6784\u8c61\u6765\u4fc3\u8fdbRTCB\u7684\u6d3b\u6027 5<\/sup>\u3002<\/li>\n\n\n\n
    • CGI-99\uff08\u4e5f\u79f0\u4e3aRTRAF\uff09<\/strong>\uff1a\u4e00\u4e2a\u4e0eFACT\u8f6c\u5f55\u590d\u5408\u7269\u540c\u6e90\u7684\u591a\u805a\u4f53\u86cb\u767d\uff0c\u53ef\u80fd\u5728\u8f6c\u5f55\u8c03\u63a7\u6216\u590d\u5408\u7269\u7684\u4e9a\u7ec6\u80de\u5b9a\u4f4d\u4e2d\u53d1\u6325\u4f5c\u7528 5<\/sup>\u3002<\/li>\n\n\n\n
    • FAM98B\u548cAshwin\uff08ASW\uff09<\/strong>\uff1a\u8fd9\u4e24\u4e2a\u4e9a\u57fa\u5728\u590d\u5408\u7269\u4e2d\u7684\u529f\u80fd\u5c1a\u4e0d\u660e\u786e\u3002Ashwin\u88ab\u53d1\u73b0\u4e0e\u722a\u87fe\u7684\u80da\u80ce\u53d1\u80b2\u6709\u5173\uff0c\u800cFAM98B\u7684\u529f\u80fd\u5219\u77e5\u4e4b\u751a\u5c11 5<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n

      tRNA-LC\u7684\u5b8c\u6574\u6027\u4f9d\u8d56\u4e8eRTCB\u4e0eDDX1\u3001CGI-99\u548cFAM98B\u7684C\u7aef\u533a\u57df\u7684\u76f8\u4e92\u4f5c\u7528 10<\/sup>\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0ctRNA-LC\u7684\u67d0\u4e9b\u4e9a\u57fa\uff08\u5982DDX1\u548cRTRAF\uff09\u8fd8\u53c2\u4e0e\u4e86\u5176\u4ed6\u7ec6\u80de\u8fc7\u7a0b\uff0c\u4f8b\u5982DNA\u53cc\u94fe\u65ad\u88c2\u4fee\u590d\u3001microRNA\uff08miRNA\uff09\u6210\u719f\u548c\u8f6c\u5f55\u8c03\u63a7 5<\/sup>\u3002\u8fd9\u4e00\u4e8b\u5b9e\u5f15\u51fa\u4e86\u4e00\u4e2a\u91cd\u8981\u7684\u63a8\u8bba\uff1atRNA-LC\u53ef\u80fd\u4e0d\u4ec5\u4ec5\u662f\u4e00\u4e2a\u9759\u6001\u7684\u3001\u4e13\u7528\u4e8etRNA\u52a0\u5de5\u7684\u673a\u5668\u3002DDX1\u7b49\u4e9a\u57fa\u7684\u591a\u529f\u80fd\u6027\u8868\u660e\uff0c\u5b83\u4eec\u6709\u53ef\u80fd\u5c06\u6574\u4e2a\u590d\u5408\u7269\uff08\u5305\u62ec\u5176\u50ac\u5316\u6838\u5fc3RTCB\uff09\u62db\u52df\u5230\u5176\u4ed6\u7ec6\u80de\u533a\u5ba4\u6216\u5206\u5b50\u901a\u8def\u4e0a\u3002\u4f8b\u5982\uff0cDDX1\u5728\u5904\u7406pre-miRNA\u6216\u4fee\u590d\u53d7\u635fRNA\u65f6\uff0c\u53ef\u80fd\u4f1a\u4ea7\u751f\u5177\u6709RTCB\u7279\u5f02\u6027\u672b\u7aef\uff08<\/p>\n\n\n\n

      3\u2032-P\/5\u2032-OH\uff09\u7684RNA\u4e2d\u95f4\u4f53\uff0c\u4ece\u800c\u4e3aRTCB\u63d0\u4f9b\u4e86\u65b0\u7684\u6f5c\u5728\u5e95\u7269\u3002\u56e0\u6b64\uff0c\u5bf9RTCB\u65b0\u5e95\u7269\u7684\u63a2\u7d22\u4e0d\u5e94\u5c40\u9650\u4e8e\u9176\u672c\u8eab\uff0c\u800c\u5e94\u5728\u6574\u4e2atRNA-LC\u7684\u529f\u80fd\u7f51\u7edc\u80cc\u666f\u4e0b\u8fdb\u884c\uff0c\u8003\u5bdf\u8be5\u590d\u5408\u7269\u88ab\u62db\u52df\u5230\u4f55\u5904\uff0c\u4ee5\u53ca\u5728\u8fd9\u4e9b\u4f4d\u7f6e\u5b58\u5728\u54ea\u4e9b\u6f5c\u5728\u7684RNA\u5e95\u7269\u3002<\/p>\n\n\n\n

      1.4 \u7ecf\u5178\u5e95\u7269<\/h3>\n\n\n\n

      RTCB\u7684\u4e24\u4e2a\u7ecf\u5178\u5e95\u7269\u662f\u5176\u529f\u80fd\u7814\u7a76\u7684\u57fa\u77f3\uff0c\u4e5f\u662f\u7406\u89e3\u5176\u975e\u526a\u63a5\u4f53\u4f9d\u8d56\u6027\u526a\u63a5\u673a\u5236\u7684\u5178\u8303\u3002<\/p>\n\n\n\n

        \n
      • tRNA\u526a\u63a5<\/strong>\uff1a\u5728\u4eba\u7c7b\u57fa\u56e0\u7ec4\u4e2d\uff0c\u7ea6\u670928\u79cdtRNA\u57fa\u56e0\u542b\u6709\u5185\u542b\u5b50\uff0c\u5176\u4e2d\u5305\u62ec\u6240\u670913\u79cd\u7f16\u7801\u916a\u6c28\u9178\u7684tRNA 5<\/sup>\u3002\u8fd9\u4e9bpre-tRNA\u7684\u6210\u719f\u8fc7\u7a0b\u9700\u8981\u7cbe\u786e\u5730\u5207\u9664\u5185\u542b\u5b50\u5e76\u8fde\u63a5\u5916\u663e\u5b50\u3002tRNA\u526a\u63a5\u5185\u5207\u9176\uff08TSEN\uff09\u590d\u5408\u7269\u9996\u5148\u8d1f\u8d23\u5207\u9664\u5185\u542b\u5b50\uff0c\u4ea7\u751f\u4e24\u4e2a\u5e26\u67092′,3′-\u73af\u78f7\u9178\u548c5′-\u7f9f\u57fa\u672b\u7aef\u7684\u5916\u663e\u5b50\u7247\u6bb5\u3002\u968f\u540e\uff0cRTCB\u4f5c\u4e3atRNA-LC\u7684\u50ac\u5316\u4e9a\u57fa\uff0c\u5c06\u8fd9\u4e24\u4e2a\u5916\u663e\u5b50\u7247\u6bb5\u51c6\u786e\u5730\u8fde\u63a5\u8d77\u6765\uff0c\u5f62\u6210\u6210\u719f\u7684\u529f\u80fd\u6027tRNA 2<\/sup>\u3002\u5728\u79c0\u4e3d\u9690\u6746\u7ebf\u866b\uff08C. elegans<\/em>\uff09\u4e2d\uff0c\u901a\u8fc7\u57fa\u56e0\u6572\u9664rtcb-1<\/em>\uff0c\u79d1\u5b66\u5bb6\u89c2\u5bdf\u5230\u672a\u8fde\u63a5\u7684tRNA\u5916\u663e\u5b50\u7247\u6bb5\u5927\u91cf\u79ef\u7d2f\uff0c\u8fd9\u76f4\u63a5\u8bc1\u660e\u4e86RTCB\u662f\u4f53\u5185tRNA\u526a\u63a5\u901a\u8def\u4e2d\u552f\u4e00\u7684\u3001\u4e0d\u53ef\u6216_S_\u7684\u8fde\u63a5\u9176 2<\/sup>\u3002<\/li>\n\n\n\n
      • XBP1 mRNA\u526a\u63a5<\/strong>\uff1a\u5728\u5185\u8d28\u7f51\uff08ER\uff09\u80c1\u8feb\u4e0b\uff0c\u7ec6\u80de\u4f1a\u542f\u52a8\u672a\u6298\u53e0\u86cb\u767d\u53cd\u5e94\uff08UPR\uff09\u4ee5\u6062\u590d\u5185\u8d28\u7f51\u7684\u7a33\u6001\u3002UPR\u4e2d\u6700\u53e4\u8001\u3001\u6700\u4fdd\u5b88\u7684\u5206\u652f\u662f\u7531IRE1\u86cb\u767d\u4ecb\u5bfc\u7684\u3002\u5f53\u5185\u8d28\u7f51\u4e2d\u672a\u6298\u53e0\u86cb\u767d\u79ef\u7d2f\u65f6\uff0cIRE1\u88ab\u6fc0\u6d3b\uff0c\u5176\u6838\u9178\u5185\u5207\u9176\u6d3b\u6027\u5728\u7ec6\u80de\u8d28\u4e2d\u7279\u5f02\u6027\u5730\u5207\u5272XBP1 mRNA\uff0c\u5207\u9664\u4e00\u4e2a26\u4e2a\u6838\u82f7\u9178\u7684\u77ed\u5185\u542b\u5b50 11<\/sup>\u3002\u8fd9\u6b21\u5207\u5272\u540c\u6837\u4ea7\u751f\u4e862′,3′-\u73af\u78f7\u9178\u548c5′-OH\u672b\u7aef\uff0c\u8fd9\u6b63\u662fRTCB\u7684\u7406\u60f3\u5e95\u7269\u3002\u957f\u671f\u4ee5\u6765\uff0c\u8d1f\u8d23\u8fde\u63a5XBP1\u5916\u663e\u5b50\u7684\u8fde\u63a5\u9176\u4e00\u76f4\u662f\u4e2a\u8c1c\uff0c\u76f4\u5230\u591a\u9879\u7814\u7a76\u6700\u7ec8\u8bc1\u5b9e\uff0cRTCB\u6b63\u662f\u6267\u884c\u8fd9\u4e00\u5173\u952e\u8fde\u63a5\u6b65\u9aa4\u7684\u9176 2<\/sup>\u3002RTCB\u4ecb\u5bfc\u7684\u8fde\u63a5\u53cd\u5e94\u4ea7\u751f\u4e86\u526a\u63a5\u540e\u7684XBP1s mRNA\uff0c\u540e\u8005\u88ab\u7ffb\u8bd1\u6210\u4e00\u4e2a\u5f3a\u6548\u7684\u8f6c\u5f55\u56e0\u5b50\uff0c\u9a71\u52a8\u4e00\u7cfb\u5217\u4e0b\u6e38\u57fa\u56e0\u7684\u8868\u8fbe\uff0c\u4ee5\u589e\u5f3a\u5185\u8d28\u7f51\u7684\u6298\u53e0\u80fd\u529b\u548c\u964d\u89e3\u80fd\u529b\uff0c\u4ece\u800c\u6062\u590d\u7ec6\u80de\u7a33\u6001 15<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n

        \u7b2c\u4e8c\u8282\uff1a\u6269\u5c55\u529f\u80fd\u5e93\uff1a\u5173\u4e8e\u65b0\u5e95\u7269\u7684\u76f4\u63a5\u4e14\u6709\u529b\u7684\u8bc1\u636e<\/h2>\n\n\n\n

        \u5c3d\u7ba1tRNA\u548cXBP1 mRNA\u662fRTCB\u5728\u540e\u751f\u52a8\u7269\u4e2d\u516c\u8ba4\u7684\u7ecf\u5178\u5e95\u7269\uff0c\u4f46\u8d8a\u6765\u8d8a\u591a\u7684\u8bc1\u636e\u8868\u660e\uff0cRTCB\u7684\u529f\u80fd\u8fdc\u4e0d\u6b62\u4e8e\u6b64\u3002\u6765\u81ea\u4e0d\u540c\u6a21\u5f0f\u751f\u7269\u7684\u7814\u7a76\uff0c\u4ece\u7ec6\u83cc\u7684RNA\u4fee\u590d\u5230\u54fa\u4e73\u52a8\u7269\u7684\u751f\u6b96\u53d1\u80b2\u548c\u795e\u7ecf\u518d\u751f\uff0c\u90fd\u63ed\u793a\u4e86RTCB\u62e5\u6709\u4e00\u4e2a\u66f4\u4e3a\u5e7f\u9614\u7684\u5e95\u7269\u8c31\u3002\u8fd9\u4e9b\u53d1\u73b0\u4e0d\u4ec5\u6269\u5c55\u4e86\u6211\u4eec\u5bf9RTCB\u751f\u7269\u5b66\u529f\u80fd\u7684\u8ba4\u77e5\uff0c\u4e5f\u6697\u793a\u5176\u53ef\u80fd\u4f5c\u4e3a\u7ec6\u80de\u8d28\u91cf\u63a7\u5236\u548c\u80c1\u8feb\u5e94\u7b54\u7f51\u7edc\u4e2d\u7684\u4e00\u4e2a\u6838\u5fc3\u8c03\u63a7\u8282\u70b9\u3002<\/p>\n\n\n\n

        2.1 \u539f\u6838\u751f\u7269RNA\u4fee\u590d\uff1a\u66f4\u5e7f\u6cdb\u5e95\u7269\u7279\u5f02\u6027\u7684\u8fdb\u5316\u903b\u8f91<\/h3>\n\n\n\n

        \u5bf9RTCB\u529f\u80fd\u591a\u6837\u6027\u7684\u63a2\u7d22\uff0c\u4e00\u4e2a\u5f3a\u6709\u529b\u7684\u903b\u8f91\u8d77\u70b9\u6765\u81ea\u4e8e\u539f\u6838\u751f\u7269\u3002\u7ec6\u83cc\u62e5\u6709RTCB\u7684\u540c\u6e90\u7269\uff0c\u4f46\u5b83\u4eec\u7684\u57fa\u56e0\u7ec4\u4e2d\u65e2\u4e0d\u542btRNA\u5185\u542b\u5b50\uff0c\u4e5f\u4e0d\u5b58\u5728IRE1-XBP1\u4ecb\u5bfc\u7684UPR\u901a\u8def 9<\/sup>\u3002\u8fd9\u4e00\u8fdb\u5316\u4e0a\u7684\u4e8b\u5b9e\u65e0\u53ef\u8fa9\u9a73\u5730\u8bc1\u660e\uff0cRTCB\u5728\u8fd9\u4e9b\u751f\u7269\u4e2d\u5fc5\u7136\u6267\u884c\u7740\u5176\u4ed6\u529f\u80fd\uff0c\u800cRNA\u4fee\u590d\u88ab\u8ba4\u4e3a\u662f\u5176\u6700\u4e3b\u8981\u7684\u804c\u8d23\u3002<\/p>\n\n\n\n

          \n
        • \u5e95\u72691\uff1a\u6838\u7cd6\u4f53RNA\uff08rRNA\uff09<\/strong>\n
            \n
          • \u5728\u591a\u79cd\u80c1\u8feb\u6761\u4ef6\u4e0b\uff0c\u4f8b\u5982\u6297\u751f\u7d20\u5904\u7406\u6216\u6bd2\u7d20-\u6297\u6bd2\u7d20\u7cfb\u7edf\uff08\u5982MazF\/MazE\uff09\u7684\u6fc0\u6d3b\uff0c\u7ec6\u83cc\u7ec6\u80de\u5185\u7684rRNA\u4f1a\u53d1\u751f\u65ad\u88c2 9<\/sup>\u3002\u8fd9\u4e9b\u65ad\u88c2\u7684rRNA\u7247\u6bb5\u4f1a\u6291\u5236\u6838\u7cd6\u4f53\u7684\u529f\u80fd\uff0c\u963b\u788d\u86cb\u767d\u8d28\u5408\u6210\u3002\u7814\u7a76\u53d1\u73b0\uff0c\u5927\u80a0\u6746\u83cc\u4e2d\u7684RTCB\u53c2\u4e0e\u4e86\u5bf9\u8fd9\u4e9b\u53d7\u635frRNA\uff08\u7279\u522b\u662f16S rRNA\uff09\u7684\u4fee\u590d\u8fc7\u7a0b 9<\/sup>\u3002<\/li>\n\n\n\n
          • \u901a\u8fc7\u6784\u5efartcB<\/em>\u57fa\u56e0\u6572\u9664\u7684\u7ec6\u83cc\u682a\uff0c\u79d1\u5b66\u5bb6\u89c2\u5bdf\u5230\uff0c\u5728\u64a4\u9500\u6297\u751f\u7d20\u80c1\u8feb\u540e\uff0c\u91ce\u751f\u578b\u83cc\u682a\u80fd\u591f\u8f83\u5feb\u6062\u590d\u751f\u957f\uff0c\u800c\u6572\u9664\u682a\u7684\u6062\u590d\u8fc7\u7a0b\u5219\u660e\u663e\u5ef6\u8fdf\u3002\u5206\u5b50\u5206\u6790\u663e\u793a\uff0c\u8fd9\u79cd\u5ef6\u8fdf\u4e0e\u6572\u9664\u682a\u4f53\u5185\u5927\u91cf\u79ef\u7d2f\u7684rRNA\u65ad\u88c2\u7247\u6bb5\u6709\u5173 18<\/sup>\u3002\u8fd9\u5f3a\u70c8\u8868\u660e\uff0cRTCB\u7684\u529f\u80fd\u662f\u91cd\u65b0\u8fde\u63a5\u8fd9\u4e9brRNA\u7247\u6bb5\uff0c\u4ece\u800c\u52a0\u901f\u529f\u80fd\u6027\u6838\u7cd6\u4f53\u7684\u518d\u751f\uff0c\u4e3a\u7ec6\u83cc\u5728\u591a\u53d8\u73af\u5883\u4e2d\u63d0\u4f9b\u4e86\u91cd\u8981\u7684\u751f\u5b58\u4f18\u52bf 18<\/sup>\u3002<\/li>\n\n\n\n
          • \u66f4\u6df1\u5165\u7684\u7814\u7a76\u8fd8\u53d1\u73b0\u4e86\u4e00\u4e2a\u9ad8\u5ea6\u7279\u5316\u7684\u7ec6\u83cc\u6838\u7cd6\u4f53\u4fee\u590d\u7cfb\u7edf\uff0c\u8be5\u7cfb\u7edf\u7531RTCB\u7684\u4e00\u4e2a\u65c1\u7cfb\u540c\u6e90\u7269\uff08RtcB2\uff09\u548c\u5176\u4f34\u4fa3\u86cb\u767dPrfH\u5171\u540c\u7ec4\u6210\u3002\u5f53\u6838\u7cd6\u4f53\u5728\u7ffb\u8bd1\u8fc7\u7a0b\u4e2d\u56e0\u89e3\u7801\u4e2d\u5fc3\u53d7\u635f\uff08\u4f8b\u5982\u88ab\u6838\u7cd6\u4f53\u6bd2\u7d20\u5207\u5272\uff09\u800c\u505c\u6ede\u65f6\uff0cPrfH\u9996\u5148\u4ecb\u5165\uff0c\u5c06\u505c\u6ede\u7684\u6838\u7cd6\u4f53\u89e3\u79bb\u6210\u5927\u5c0f\u4e9a\u57fa\uff0c\u4ece\u800c\u66b4\u973216S rRNA\u4e0a\u7684\u635f\u4f24\u4f4d\u70b9\u3002\u968f\u540e\uff0cRtcB2\u624d\u80fd\u63a5\u8fd1\u5e76\u8fde\u63a5\u65ad\u88c2\u768416S rRNA\uff0c\u5b8c\u6210\u4fee\u590d 19<\/sup>\u3002\u8fd9\u662f\u4e00\u4e2a\u7cbe\u5bc6\u7684\u3001\u5206\u5de5\u660e\u786e\u7684\u6838\u7cd6\u4f53\u62ef\u6551\u4e0e\u4fee\u590d\u673a\u5236\u3002<\/li>\n<\/ul>\n<\/li>\n\n\n\n
          • \u5e95\u72692\uff1a\u8f6c\u79fb-\u4fe1\u4f7fRNA\uff08tmRNA, ssrA<\/em>\uff09<\/strong>\n
              \n
            • tmRNA\uff08\u7531ssrA<\/em>\u57fa\u56e0\u7f16\u7801\uff09\u662f\u7ec6\u83cc\u4e2d\u4e00\u79cd\u72ec\u7279\u7684RNA\u5206\u5b50\uff0c\u517c\u5177tRNA\u548cmRNA\u7684\u529f\u80fd\uff0c\u5176\u4e3b\u8981\u4f5c\u7528\u662f\u8bc6\u522b\u5e76\u89e3\u6551\u505c\u6ede\u5728mRNA\u4e0a\u7684\u6838\u7cd6\u4f53\uff0c\u8fd9\u662f\u4e00\u4e2a\u5173\u952e\u7684\u7ffb\u8bd1\u8d28\u91cf\u63a7\u5236\u8fc7\u7a0b 23<\/sup>\u3002<\/li>\n\n\n\n
            • \u901a\u8fc7\u4e00\u79cd\u57fa\u4e8e\u8fde\u63a5\u53cd\u5e94\u7684\u7b5b\u9009\u6280\u672f\uff0c\u7814\u7a76\u4eba\u5458\u8bd5\u56fe\u5728\u5927\u80a0\u6746\u83cc\u4e2d\u9274\u5b9aRTCB\u7684\u6240\u6709\u6f5c\u5728\u5e95\u7269\u3002\u7ed3\u679c\u663e\u793a\uff0c\u5728\u4f17\u591a\u88ab\u6355\u83b7\u7684RNA\u7247\u6bb5\u4e2d\uff0cssrA<\/em>\u662f\u88ab\u9274\u5b9a\u5230\u9891\u7387\u6700\u9ad8\u7684\u6f5c\u5728\u5e95\u7269\u4e4b\u4e00 5<\/sup>\u3002<\/li>\n\n\n\n
            • \u8003\u8651\u5230RTCB\u5728rRNA\u4fee\u590d\u4e2d\u7684\u4f5c\u7528\uff0c\u5176\u4e0etmRNA\u7684\u6f5c\u5728\u8054\u7cfb\u8fdb\u4e00\u6b65\u5de9\u56fa\u4e86\u5b83\u4f5c\u4e3a\u7ef4\u6301\u7ffb\u8bd1\u4fdd\u771f\u5ea6\u548c\u6548\u7387\u7684\u5173\u952e\u56e0\u5b50\u7684\u5730\u4f4d\u3002RTCB\u53ef\u80fd\u901a\u8fc7\u4fee\u590d\u53d7\u635f\u7684tmRNA\uff0c\u6216\u53c2\u4e0e\u5176\u52a0\u5de5\u8fc7\u7a0b\uff0c\u6765\u786e\u4fdd\u7ffb\u8bd1\u8d28\u91cf\u63a7\u5236\u7cfb\u7edf\u7684\u6b63\u5e38\u8fd0\u4f5c\uff0c\u5c24\u5176\u662f\u5728\u80c1\u8feb\u6761\u4ef6\u4e0b 22<\/sup>\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n

              2.2 \u540e\u751f\u52a8\u7269mRNA\u6210\u719f\uff1a\u5728\u8c03\u63a7\u6027\u5185\u542b\u5b50\u4fdd\u7559\u4e2d\u7684\u4f5c\u7528<\/h3>\n\n\n\n

              \u9664\u4e86\u7ecf\u5178\u7684\u5916\u663e\u5b50\u8fde\u63a5\u529f\u80fd\uff0cRTCB\u8fd8\u5728\u4e00\u79cd\u88ab\u79f0\u4e3a\u5185\u542b\u5b50\u4fdd\u7559\uff08Intron Retention, IR\uff09\u7684\u975e\u7ecf\u5178\u9009\u62e9\u6027\u526a\u63a5\u4e2d\u626e\u6f14\u7740\u4ee4\u4eba\u60ca\u8bb6\u7684\u89d2\u8272 24<\/sup>\u3002IR\u662f\u6307\u5728\u6210\u719f\u7684mRNA\u4e2d\uff0c\u4e00\u4e2a\u6216\u591a\u4e2a\u5185\u542b\u5b50\u88ab\u5b8c\u6574\u5730\u4fdd\u7559\u4e0b\u6765\uff0c\u8fd9\u662f\u4e00\u79cd\u91cd\u8981\u7684\u57fa\u56e0\u8868\u8fbe\u8c03\u63a7\u65b9\u5f0f\u3002<\/p>\n\n\n\n

                \n
              • \u6765\u81ea\u6761\u4ef6\u6027Rtcb<\/em>\u57fa\u56e0\u6572\u9664\u5c0f\u9f20\u7684\u7814\u7a76\u63d0\u4f9b\u4e86\u5f3a\u6709\u529b\u7684\u8bc1\u636e\u3002\u5728\u5375\u6bcd\u7ec6\u80de\u4e2d\u7279\u5f02\u6027\u6572\u9664Rtcb<\/em>\u540e\uff0c\u96cc\u6027\u5c0f\u9f20\u8868\u73b0\u51fa\u4e25\u91cd\u7684\u5375\u5de2\u65e9\u8870\u8868\u578b\uff0c\u5176\u5375\u6bcd\u7ec6\u80de\u65e0\u6cd5\u6b63\u5e38\u6210\u719f\uff0c\u6700\u7ec8\u5bfc\u81f4\u4e0d\u80b2 26<\/sup>\u3002<\/li>\n\n\n\n
              • \u5bf9\u8fd9\u4e00\u8868\u578b\u7684\u5206\u5b50\u673a\u5236\u8fdb\u884c\u63a2\u7a76\u540e\u53d1\u73b0\uff0c\u5176\u6839\u672c\u539f\u56e0\u5728\u4e8eRtcb<\/em>\u6572\u9664\u7684\u5375\u6bcd\u7ec6\u80de\u4e2d\uff0c\u5927\u91cf\u6bcd\u6e90mRNA\u51fa\u73b0\u4e86\u5185\u542b\u5b50\u4fdd\u7559\u7684\u526a\u63a5\u7f3a\u9677\u3002\u81f3\u5173\u91cd\u8981\u7684\u662f\uff0c\u8fd9\u4e9b\u53d7\u5f71\u54cd\u7684\u8f6c\u5f55\u672c\u5e76\u975e\u968f\u673a\u5206\u5e03\uff0c\u800c\u662f\u663e\u8457\u5bcc\u96c6\u5728\u4e24\u7c7b\u5173\u952e\u7684\u529f\u80fd\u901a\u8def\u4e2d\uff1aDNA\u7532\u57fa\u5316<\/strong>\u548cDNA\u635f\u4f24\u5e94\u7b54\uff08DDR\uff09<\/strong> 5<\/sup>\u3002<\/li>\n\n\n\n
              • \u8fd9\u4e9b\u5173\u952e\u8c03\u63a7\u86cb\u767d\u7684mRNA\u526a\u63a5\u5f02\u5e38\uff0c\u5bfc\u81f4\u5176\u86cb\u767d\u8868\u8fbe\u6c34\u5e73\u4e0b\u964d\uff0c\u4ece\u800c\u5f15\u53d1\u4e86\u4e00\u7cfb\u5217\u8fde\u9501\u53cd\u5e94\uff0c\u5305\u62ec\u65e0\u6cd5\u6b63\u5e38\u6fc0\u6d3b\u5bf9\u6062\u590d\u51cf\u6570\u5206\u88c2\u81f3\u5173\u91cd\u8981\u7684CDK1\u6fc0\u9176\uff0c\u4ee5\u53ca\u6291\u5236\u5408\u5b50\u57fa\u56e0\u7ec4\u7684\u6fc0\u6d3b\uff08ZGA\uff09\uff0c\u6700\u7ec8\u5bfc\u81f4\u4e86\u5375\u6bcd\u7ec6\u80de\u53d1\u80b2\u505c\u6ede 26<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n

                \u8fd9\u4e00\u53d1\u73b0\u63ed\u793a\u4e86RTCB\u7684\u4e00\u79cd\u5168\u65b0\u8c03\u63a7\u6a21\u5f0f\uff0c\u4f46\u5176\u5177\u4f53\u673a\u5236\u4ecd\u6709\u5f85\u9610\u660e\u3002\u4e00\u79cd\u53ef\u80fd\u7684\u201c\u76f4\u63a5\u6a21\u578b\u201d\u5047\u8bbe\uff0c\u8fd9\u4e9b\u7279\u5b9a\u7684DDR\u548c\u7532\u57fa\u5316\u76f8\u5173\u57fa\u56e0\u7684pre-mRNA\uff0c\u53ef\u80fd\u50cfXBP1\u4e00\u6837\uff0c\u88ab\u67d0\u79cdIRE1\u6837\u7684\u5185\u5207\u9176\u5207\u5272\u5185\u542b\u5b50\uff0c\u4ea7\u751f\u9700\u8981RTCB\u8fde\u63a5\u7684\u5916\u663e\u5b50\u3002\u5728RTCB\u7f3a\u5931\u7684\u60c5\u51b5\u4e0b\uff0c\u5916\u663e\u5b50\u65e0\u6cd5\u8fde\u63a5\uff0c\u5bfc\u81f4\u5185\u542b\u5b50\u4fdd\u7559\u7684\u8f6c\u5f55\u672c\u79ef\u7d2f\u3002\u53e6\u4e00\u79cd\u201c\u95f4\u63a5\u6a21\u578b\u201d\u5219\u8ba4\u4e3a\uff0cRTCB\u7684\u7f3a\u5931\u5bfc\u81f4\u7279\u5b9atRNA\uff08\u5982\u916a\u6c28\u9178tRNA 5<\/sup>\uff09\u7684\u6210\u719f\u969c\u788d\uff0c\u8fdb\u800c\u5f15\u8d77\u5728\u7279\u5b9a\u5bc6\u7801\u5b50\u4e0a\u7684\u7ffb\u8bd1\u505c\u6ede\uff0c\u8fd9\u79cd\u505c\u6ede\u53ef\u80fd\u901a\u8fc7\u67d0\u79cd\u53cd\u9988\u673a\u5236\uff0c\u5f71\u54cd\u4e86\u526a\u63a5\u4f53\u5bf9\u7279\u5b9amRNA\u7684\u526a\u63a5\u6548\u7387\u3002\u65e0\u8bba\u5177\u4f53\u673a\u5236\u5982\u4f55\uff0cRTCB\u5728\u8c03\u63a7\u5185\u542b\u5b50\u4fdd\u7559\u4e2d\u7684\u4f5c\u7528\uff0c\u4ee3\u8868\u4e86\u4e0d\u540cRNA\u52a0\u5de5\u9014\u5f84\u4e4b\u95f4\u76f8\u4e92\u534f\u8c03\u7684\u4e00\u4e2a\u524d\u6cbf\u9886\u57df\u3002<\/p>\n\n\n\n

                2.3 \u795e\u7ecf\u5143\u529f\u80fd\uff1a\u8fde\u63a5\u4e00\u79cd\u672a\u77e5\u7684\u8f74\u7a81\u518d\u751f\u6291\u5236\u56e0\u5b50<\/h3>\n\n\n\n

                \u5728\u795e\u7ecf\u7cfb\u7edf\u4e2d\uff0cRTCB\u7684\u529f\u80fd\u5448\u73b0\u51fa\u4e00\u79cd\u5f15\u4eba\u6df1\u601d\u7684\u6096\u8bba\u3002\u4e00\u65b9\u9762\uff0c\u5b83\u901a\u8fc7\u7ecf\u5178\u7684XBP-1\u901a\u8def\u5728\u795e\u7ecf\u4fdd\u62a4\u4e2d\u53d1\u6325\u7740\u81f3\u5173\u91cd\u8981\u7684\u4f5c\u7528 28<\/sup>\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u5728\u79c0\u4e3d\u9690\u6746\u7ebf\u866b\u4e2d\u7684\u7814\u7a76\u53d1\u73b0\uff0cRTCB\u7684\u8fde\u63a5\u9176\u6d3b\u6027\u672c\u8eab\u5374\u5bf9\u8f74\u7a81\u635f\u4f24\u540e\u7684\u518d\u751f\u8d77\u5230\u4e86<\/p>\n\n\n\n

                \u6291\u5236<\/strong>\u4f5c\u7528 32<\/sup>\u3002<\/p>\n\n\n\n

                \u8fd9\u4e00\u53d1\u73b0\u4e3aRTCB\u5b58\u5728\u65b0\u5e95\u7269\u63d0\u4f9b\u4e86\u8fc4\u4eca\u4e3a\u6b62\u6700\u5f3a\u7684\u9057\u4f20\u5b66\u8bc1\u636e\uff1a<\/p>\n\n\n\n

                  \n
                1. \u8868\u578b\u8bc1\u636e<\/strong>\uff1artcb-1<\/em>\u57fa\u56e0\u7a81\u53d8\u7684\u7ebf\u866b\uff0c\u5728\u906d\u53d7\u6fc0\u5149\u8f74\u7a81\u5207\u65ad\u672f\u540e\uff0c\u8868\u73b0\u51fa\u6bd4\u91ce\u751f\u578b\u66f4\u5feb\u3001\u66f4\u6210\u529f\u7684\u8f74\u7a81\u518d\u751f\u80fd\u529b 32<\/sup>\u3002<\/li>\n\n\n\n
                2. \u50ac\u5316\u6d3b\u6027\u4f9d\u8d56\u6027<\/strong>\uff1a\u8fd9\u79cd\u6291\u5236\u4f5c\u7528\u4e25\u683c\u4f9d\u8d56\u4e8eRTCB\u7684\u8fde\u63a5\u9176\u6d3b\u6027<\/strong>\u3002\u5982\u679c\u5728rtcb-1<\/em>\u7a81\u53d8\u4f53\u4e2d\u91cd\u65b0\u8868\u8fbe\u4e00\u4e2a\u50ac\u5316\u6d3b\u6027\u5931\u6d3b\u7684RTCB\u86cb\u767d\uff08\u4f8b\u5982\uff0c\u901a\u8fc7H428A\u70b9\u7a81\u53d8\uff09\uff0c\u5219\u65e0\u6cd5\u6062\u590d\u5bf9\u8f74\u7a81\u518d\u751f\u7684\u6291\u5236\u6548\u5e94 32<\/sup>\u3002<\/li>\n\n\n\n
                3. \u901a\u8def\u89e3\u8026<\/strong>\uff1a\u6700\u5173\u952e\u7684\u8bc1\u636e\u5728\u4e8e\uff0c\u8fd9\u4e00\u529f\u80fd\u4e0e\u5df2\u77e5\u7684tRNA\u548cUPR\u901a\u8def\u662f\u5b8c\u5168\u72ec\u7acb<\/strong>\u7684\u3002\u7814\u7a76\u4eba\u5458\u5728rtcb-1<\/em>\u7a81\u53d8\u4f53\u4e2d\u7279\u5f02\u6027\u8868\u8fbe\u5df2\u7ecf\u526a\u63a5\u597d\u7684\u3001\u5177\u6709\u6d3b\u6027\u7684XBP-1s\u86cb\u767d\uff0c\u867d\u7136\u8fd9\u6210\u529f\u5730\u6062\u590d\u4e86UPR\u901a\u8def\u7684\u529f\u80fd\uff0c\u4f46\u5bf9\u589e\u5f3a\u7684\u8f74\u7a81\u518d\u751f\u8868\u578b\u5374\u6beb\u65e0\u5f71\u54cd 2<\/sup>\u3002\u8fd9\u4e2a\u7cbe\u5de7\u7684\u5b9e\u9a8c\u8bbe\u8ba1\uff0c\u51b3\u5b9a\u6027\u5730\u5c06RTCB\u5728\u8f74\u7a81\u518d\u751f\u4e2d\u7684\u4f5c\u7528\u4e0e\u5176\u5728UPR\u4e2d\u7684\u4f5c\u7528\u89e3\u8026\u3002<\/li>\n<\/ol>\n\n\n\n

                  \u7efc\u5408\u8fd9\u4e9b\u8bc1\u636e\uff0c\u552f\u4e00\u5408\u7406\u7684\u7ed3\u8bba\u662f\uff1aRTCB\u5728\u795e\u7ecf\u5143\u4e2d\u8fde\u63a5\u4e86\u4e00\u4e2a\u6216\u591a\u4e2a\u201c\u8fc4\u4eca\u672a\u77e5\u7684\u5e95\u7269\u201d\uff0c\u8fd9\u4e9b\u5e95\u7269\u5728\u88abRTCB\u52a0\u5de5\uff08\u8fde\u63a5\uff09\u540e\uff0c\u4f1a\u8f6c\u53d8\u4e3a\u4e00\u79cd\u80fd\u591f\u6291\u5236\u795e\u7ecf\u5143\u5185\u5728\u518d\u751f\u80fd\u529b\u7684\u5206\u5b50 5<\/sup>\u3002\u6b64\u5916\uff0c\u7814\u7a76\u8fd8\u89c2\u5bdf\u5230\uff0c\u5728\u8f74\u7a81\u635f\u4f24\u540e\uff0cRTCB\u86cb\u767d\u4f1a\u4ece\u795e\u7ecf\u5143\u80de\u4f53\u8f6c\u79fb\u5e76\u5bcc\u96c6\u5230\u53d7\u635f\u7684\u8f74\u7a81\u672b\u7aef\uff0c\u8fd9\u8fdb\u4e00\u6b65\u6697\u793a\u4e86\u5728\u635f\u4f24\u5c40\u90e8\u53d1\u751f\u4e86\u4e00\u6b21\u7279\u5f02\u6027\u7684RNA\u52a0\u5de5\u4e8b\u4ef6 32<\/sup>\u3002<\/p>\n\n\n\n

                  \u7efc\u5408\u6765\u770b\uff0c\u4ece\u7ec6\u83cc\u5230\u540e\u751f\u52a8\u7269\uff0cRTCB\u7684\u529f\u80fd\u8c31\u7cfb\u6307\u5411\u4e00\u4e2a\u5171\u540c\u7684\u4e3b\u9898\uff1a\u5b83\u5e76\u4e0d\u4ec5\u4ec5\u662f\u4e00\u4e2a\u53c2\u4e0etRNA\u5408\u6210\u7684\u7ba1\u5bb6\u9176\uff0c\u800c\u662f\u4e00\u4e2a\u5728\u591a\u79cd\u7ec6\u80de\u8d28\u91cf\u63a7\u5236\u548c\u80c1\u8feb\u5e94\u7b54\u901a\u8def\u4e2d\u53d1\u6325\u6838\u5fc3\u4f5c\u7528\u7684\u6548\u5e94\u9176\u3002\u5176\u5e95\u7269\u901a\u5e38\u662f\u5728\u54cd\u5e94\u7279\u5b9a\u4fe1\u53f7\u6216\u635f\u4f24\u4e8b\u4ef6\u540e\u88ab\u5207\u5272\u4ea7\u751f\u7684RNA\u5206\u5b50\u3002\u65e0\u8bba\u662f\u4fee\u590d\u53d7\u635f\u7684\u7ffb\u8bd1\u673a\u5668\uff08rRNA, tmRNA\uff09\uff0c\u8fd8\u662f\u786e\u4fdd\u57fa\u56e0\u7ec4\u7a33\u5b9a\u6027\u7684\u7ef4\u62a4\uff08DDR\u76f8\u5173mRNA\uff09\uff0c\u4ea6\u6216\u662f\u54cd\u5e94\u7ec6\u80de\u5185\u7a33\u6001\u5931\u8861\uff08XBP1\uff09\u548c\u7269\u7406\u635f\u4f24\uff08\u672a\u77e5\u7684\u8f74\u7a81\u518d\u751f\u6291\u5236\u56e0\u5b50\uff09\uff0cRTCB\u90fd\u626e\u6f14\u7740\u4e00\u4e2a\u4e0d\u53ef\u6216\u7f3a\u7684\u201c\u5206\u5b50\u5916\u79d1\u533b\u751f\u201d\u7684\u89d2\u8272\u3002<\/p>\n\n\n\n

                  \u7b2c\u4e09\u8282\uff1aRTCB\u751f\u7269\u5b66\u7684\u524d\u6cbf\uff1a\u63a8\u6d4b\u6027\u4f5c\u7528\u4e0e\u975e\u5e38\u89c4\u6d3b\u6027<\/h2>\n\n\n\n

                  \u5728RTCB\u7814\u7a76\u7684\u7248\u56fe\u4e0a\uff0c\u9664\u4e86\u6709\u76f4\u63a5\u8bc1\u636e\u652f\u6301\u7684\u65b0\u5e95\u7269\u5916\uff0c\u8fd8\u5b58\u5728\u4e00\u4e9b\u66f4\u5177\u63a8\u6d4b\u6027\u4f46\u540c\u6837\u5f15\u4eba\u5165\u80dc\u7684\u529f\u80fd\u9886\u57df\u3002\u8fd9\u4e9b\u9886\u57df\u6d89\u53ca\u5176\u4ed6\u975e\u7f16\u7801RNA\u3001\u5bf9DNA\u7684\u76f4\u63a5\u4f5c\u7528\uff0c\u751a\u81f3\u5b8c\u5168\u4e0d\u4f9d\u8d56\u5176\u8fde\u63a5\u9176\u6d3b\u6027\u7684\u529f\u80fd\u3002\u63a2\u7d22\u8fd9\u4e9b\u524d\u6cbf\u9886\u57df\uff0c\u5bf9\u4e8e\u5168\u9762\u7406\u89e3RTCB\u4f5c\u4e3a\u4e00\u79cd\u591a\u529f\u80fd\u5206\u5b50\u7684\u590d\u6742\u6027\u81f3\u5173\u91cd\u8981\u3002<\/p>\n\n\n\n

                  3.1 \u5176\u4ed6\u975e\u7f16\u7801RNA\uff08lncRNA, miRNA, circRNA\uff09\u7684\u95ee\u9898<\/h3>\n\n\n\n

                  \u968f\u7740\u5bf9\u975e\u7f16\u7801RNA\uff08ncRNA\uff09\u4e16\u754c\u7684\u6df1\u5165\u63a2\u7d22\uff0c\u4e00\u4e2a\u81ea\u7136\u800c\u7136\u7684\u95ee\u9898\u662f\uff1aRTCB\u662f\u5426\u53c2\u4e0e\u4e86\u957f\u975e\u7f16\u7801RNA\uff08lncRNA\uff09\u3001\u5fae\u5c0fRNA\uff08miRNA\uff09\u6216\u73af\u72b6RNA\uff08circRNA\uff09\u7684\u52a0\u5de5\u6216\u526a\u63a5\uff1f<\/p>\n\n\n\n

                    \n
                  • \u76ee\u524d\u5c1a\u65e0\u76f4\u63a5\u8bc1\u636e<\/strong>\uff1a\u6839\u636e\u73b0\u6709\u7814\u7a76\uff0c\u76ee\u524d\u6ca1\u6709\u4efb\u4f55\u76f4\u63a5\u7684\u751f\u5316\u6216\u9057\u4f20\u5b66\u8bc1\u636e\u8868\u660eRTCB\u7684\u8fde\u63a5\u9176\u6d3b\u6027\u76f4\u63a5\u53c2\u4e0e\u4e86\u8fd9\u4e9b\u975e\u7f16\u7801RNA\u7684\u751f\u7269\u5408\u6210\u6216\u526a\u63a5\u8fc7\u7a0b 36<\/sup>\u3002\u8fd9\u4e9bncRNA\u7684\u52a0\u5de5\u901a\u5e38\u4f9d\u8d56\u4e8e\u5176\u4ed6\u6210\u719f\u7684\u3001\u7279\u5f02\u6027\u7684\u901a\u8def\u3002\u4f8b\u5982\uff0cmiRNA\u7684\u751f\u6210\u4f9d\u8d56\u4e8eDrosha\u548cDicer\u9176\u7684\u5207\u5272\uff0c\u800c\u5927\u591a\u6570circRNA\u5219\u662f\u7531\u526a\u63a5\u4f53\u901a\u8fc7\u201c\u53cd\u5411\u526a\u63a5\u201d\uff08back-splicing\uff09\u673a\u5236\u4ea7\u751f\u7684 41<\/sup>\u3002<\/li>\n\n\n\n
                  • \u95f4\u63a5\u8054\u7cfb\u7684\u53ef\u80fd\u6027<\/strong>\uff1a\u5c3d\u7ba1\u7f3a\u4e4f\u76f4\u63a5\u8bc1\u636e\uff0c\u4f46\u4e00\u4e2a\u95f4\u63a5\u7684\u8054\u7cfb\u6765\u81ea\u4e8eRTCB\u6240\u5728\u7684tRNA\u8fde\u63a5\u9176\u590d\u5408\u7269\uff08tRNA-LC\uff09\u3002\u5982\u524d\u6240\u8ff0\uff0ctRNA-LC\u4e2d\u7684\u4e00\u4e2a\u5173\u952e\u4e9a\u57fa\u2014\u2014RNA\u89e3\u65cb\u9176DDX1<\/strong>\uff0c\u5df2\u88ab\u8bc1\u5b9e\u53c2\u4e0e\u4e86\u67d0\u4e9bmiRNA<\/strong>\u524d\u4f53\uff08pre-miRNA\uff09\u7684\u52a0\u5de5\u8fc7\u7a0b 5<\/sup>\u3002\u8fd9\u4e00\u4e8b\u5b9e\u610f\u5473\u7740\uff0cRTCB\u5728\u7269\u7406\u4e0a\u53ef\u80fd\u88ab\u5e26\u5230miRNA\u52a0\u5de5\u7684\u573a\u6240\u3002\u867d\u7136\u8fd9\u4e3aRTCB\u53ef\u80fd\u63a5\u89e6\u5230miRNA\u4e2d\u95f4\u4f53\u63d0\u4f9b\u4e86\u53ef\u80fd\u6027\uff0c\u4f46\u5176\u662f\u5426\u5728\u5176\u4e2d\u53d1\u6325\u50ac\u5316\u4f5c\u7528\uff0c\u76ee\u524d\u5b8c\u5168\u505c\u7559\u5728\u63a8\u6d4b\u5c42\u9762\u3002<\/li>\n\n\n\n
                  • \u5bf9circRNA\u7684\u5047\u8bbe<\/strong>\uff1a\u5bf9\u4e8e\u73af\u72b6RNA\uff0c\u867d\u7136\u5176\u4e3b\u8981\u6765\u6e90\u662f\u526a\u63a5\u4f53\uff0c\u4f46\u7406\u8bba\u4e0a\u5b58\u5728\u4e00\u79cd\u66ff\u4ee3\u6027\u7684\u751f\u6210\u9014\u5f84\uff1a\u4e00\u4e2a\u7ebf\u6027\u7684RNA\u5206\u5b50\u88ab\u67d0\u79cd\u5185\u5207\u9176\u5207\u5272\uff0c\u4ea7\u751f\u5339\u914d\u76843′-P\u548c5′-OH\u672b\u7aef\uff0c\u7136\u540e\u7531RTCB\u8fdb\u884c\u5206\u5b50\u5185\u8fde\u63a5\uff0c\u5f62\u6210\u73af\u72b6\u7ed3\u6784\u3002\u8fd9\u4ecd\u7136\u662f\u4e00\u4e2a\u6709\u5f85\u672a\u6765\u7814\u7a76\u9a8c\u8bc1\u7684\u5047\u8bf4\u3002<\/li>\n<\/ul>\n\n\n\n

                    3.2 DNA\u7684\u8fde\u63a5\u9176\uff1fDNA\u672b\u7aef\u52a0\u5e3d\u6d3b\u6027\u7684\u51fa\u73b0<\/h3>\n\n\n\n

                    \u5728RTCB\u7684\u7814\u7a76\u4e2d\uff0c\u6700\u4ee4\u4eba\u60ca\u8bb6\u7684\u53d1\u73b0\u4e4b\u4e00\u662f\u5176\u8de8\u8d8aRNA-DNA\u754c\u9650\u7684\u80fd\u529b\u3002\u5728\u7ec6\u83cc\uff08\u5982\u5927\u80a0\u6746\u83cc\u548c\u6c99\u95e8\u6c0f\u83cc\uff09\u4e2d\uff0c\u7814\u7a76\u63ed\u793a\u4e86RTCB\u62e5\u6709\u4e00\u79cd\u524d\u6240\u672a\u89c1\u7684\u201cDNA\u672b\u7aef\u52a0\u5e3d\u201d\uff08DNA capping\uff09\u6d3b\u6027 5<\/sup>\u3002<\/p>\n\n\n\n

                      \n
                    • \u673a\u5236\u4e0e\u529f\u80fd<\/strong>\uff1a\u8fd9\u79cd\u6d3b\u6027\u662f\u6307RTCB\u80fd\u591f\u5c06\u4e00\u4e2a\u9e1f\u82f7\u9178\uff08GMP\uff09\u57fa\u56e2\u52a0\u5230\u65ad\u88c2DNA\u94fe\u76843′-\u78f7\u9178\u672b\u7aef\uff0c\u5f62\u6210\u4e00\u4e2a\u72ec\u7279\u7684DNA-3’pp5’G\u5e3d\u5b50\u7ed3\u6784\u3002\u8fd9\u4e2a\u5e3d\u5b50\u7ed3\u6784\u88ab\u8ba4\u4e3a\u80fd\u591f\u4fdd\u62a4DNA\u672b\u7aef\u514d\u53d7\u6838\u9178\u5916\u5207\u9176\u7684\u964d\u89e3\uff0c\u4ece\u800c\u5728\u7ec6\u80de\u906d\u53d7\u57fa\u56e0\u6bd2\u6027\u80c1\u8feb\u65f6\uff0c\u4e3a\u7ef4\u6301\u57fa\u56e0\u7ec4\u7684\u7a33\u5b9a\u6027\u63d0\u4f9b\u4e86\u4e00\u5c42\u989d\u5916\u7684\u4fdd\u62a4 5<\/sup>\u3002<\/li>\n\n\n\n
                    • \u4e0eRNA\u8fde\u63a5\u7684\u5171\u6027<\/strong>\uff1a\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u5c3d\u7ba1\u4f5c\u7528\u4e8eDNA\u5e95\u7269\uff0c\u8fd9\u79cd\u52a0\u5e3d\u6d3b\u6027\u4ecd\u7136\u5229\u7528\u4e86RTCB\u6838\u5fc3\u7684\u50ac\u5316\u673a\u5236\uff0c\u5373\u4f9d\u8d56GTP\u7684\u9176\u81ea\u8eab\u9e1f\u82f7\u9170\u5316\u8fc7\u7a0b\u3002\u8fd9\u8868\u660eRTCB\u7684\u50ac\u5316\u6838\u5fc3\u5177\u6709\u4e00\u5b9a\u7684\u5e95\u7269\u7075\u6d3b\u6027\u3002<\/li>\n\n\n\n
                    • \u5173\u952e\u7684\u8b66\u793a<\/strong>\uff1a\u5fc5\u987b\u5f3a\u8c03\u7684\u662f\uff0c\u8fd9\u79cdDNA\u52a0\u5e3d\u529f\u80fd\u76ee\u524d\u5c1a\u672a\u5728\u4efb\u4f55\u771f\u6838\u751f\u7269\u4e2d\u5f97\u5230\u8bc1\u5b9e<\/strong>\u3002\u7136\u800c\uff0c\u5b83\u5728\u539f\u6838\u751f\u7269\u4e2d\u7684\u5b58\u5728\uff0c\u4e3aRTCB\u7684\u7814\u7a76\u5f00\u8f9f\u4e86\u4e00\u4e2a\u5168\u65b0\u7684\u65b9\u5411\uff0c\u5e76\u5f3a\u70c8\u6697\u793aRTCB\u53ef\u80fd\u5728DNA\u635f\u4f24\u5e94\u7b54\uff08DDR\uff09\u4e2d\u626e\u6f14\u7740\u76f4\u63a5\u7684\u89d2\u8272 5<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n

                      \u8fd9\u4e00\u53d1\u73b0\u4e0e\u524d\u8ff0RTCB\u5728\u5c0f\u9f20\u5375\u6bcd\u7ec6\u80de\u4e2d\u8c03\u63a7DDR\u76f8\u5173mRNA\u526a\u63a5\u7684\u529f\u80fd\u5f62\u6210\u4e86\u6709\u8da3\u7684\u547c\u5e94\u3002\u8fd9\u4e24\u6761\u6765\u81ea\u4e0d\u540c\u6a21\u5f0f\u751f\u7269\u3001\u770b\u4f3c\u72ec\u7acb\u7684\u8bc1\u636e\uff0c\u5171\u540c\u6307\u5411\u4e86\u4e00\u4e2a\u5b8f\u5927\u7684\u4e3b\u9898\uff1aRTCB\u5728\u7ef4\u6301\u57fa\u56e0\u7ec4\u5b8c\u6574\u6027\u4e2d\u53ef\u80fd\u626e\u6f14\u7740\u591a\u5c42\u6b21\u7684\u89d2\u8272\u3002\u53ef\u4ee5\u8bbe\u60f3\uff0c\u5728\u771f\u6838\u7ec6\u80de\u4e2d\uff0cRTCB\u53ef\u80fd\u62e5\u6709\u4e00\u79cd\u53cc\u91cdDDR\u529f\u80fd\uff1a\u4e00\u65b9\u9762\uff0c\u53ef\u80fd\u5b58\u5728\u4e00\u79cd\u4fdd\u5b88\u7684\u3001\u76f4\u63a5\u7684DNA\u52a0\u5e3d\u6d3b\u6027\uff0c\u5728DNA\u65ad\u88c2\u4f4d\u70b9\u63d0\u4f9b\u5feb\u901f\u7684\u4fdd\u62a4\uff1b\u53e6\u4e00\u65b9\u9762\uff0c\u901a\u8fc7\u8c03\u63a7\u76f8\u5173mRNA\u7684\u526a\u63a5\uff0c\u786e\u4fdd\u7ec6\u80de\u62e5\u6709\u5145\u8db3\u7684\u3001\u7528\u4e8e\u4fee\u590d\u7684\u86cb\u767d\u8d28\u5de5\u5177\u7bb1\u3002\u9a8c\u8bc1RTCB\u5728\u4eba\u7c7b\u7ec6\u80de\u4e2d\u662f\u5426\u4e5f\u5177\u6709DNA\u52a0\u5e3d\u529f\u80fd\uff0c\u5c06\u662f\u8be5\u9886\u57df\u672a\u6765\u4e00\u4e2a\u91cd\u5927\u7684\u7a81\u7834\u65b9\u5411\u3002<\/p>\n\n\n\n

                      3.3 \u5728\u5148\u5929\u6027\u514d\u75ab\u4e2d\u7684\u975e\u526a\u63a5\u4f9d\u8d56\u6027\u652f\u67b6\u4f5c\u7528<\/h3>\n\n\n\n

                      RTCB\u7684\u529f\u80fd\u591a\u6837\u6027\u4e0d\u4ec5\u4f53\u73b0\u5728\u5176\u5e95\u7269\u7684\u591a\u6837\u6027\u4e0a\uff0c\u8fd8\u4f53\u73b0\u5728\u5176\u53ef\u4ee5\u5b8c\u5168\u4e0d\u4f9d\u8d56\u50ac\u5316\u6d3b\u6027\uff0c\u800c\u901a\u8fc7\u86cb\u767d\u8d28-\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u53d1\u6325\u529f\u80fd\u3002\u7532\u578b\u6d41\u611f\u75c5\u6bd2\uff08IAV\uff09\u611f\u67d3\u7684\u7814\u7a76\u63d0\u4f9b\u4e86\u4e00\u4e2a\u7edd\u4f73\u7684\u4f8b\u8bc1 42<\/sup>\u3002<\/p>\n\n\n\n

                        \n
                      • \u514d\u75ab\u6291\u5236\u673a\u5236<\/strong>\uff1a\u5728\u5bbf\u4e3b\u7ec6\u80de\u4e2d\uff0cRNA\u89e3\u65cb\u9176DDX1\u662f\u4e00\u4e2a\u91cd\u8981\u7684\u6a21\u5f0f\u8bc6\u522b\u53d7\u4f53\uff0c\u80fd\u591f\u611f\u77e5\u75c5\u6bd2RNA\u7684\u5b58\u5728\u3002\u5728\u88ab\u6fc0\u6d3b\u540e\uff0cDDX1\u4f1a\u4e0e\u53e6\u4e00\u4e2a\u89e3\u65cb\u9176DDX21\u5f62\u6210\u590d\u5408\u7269\uff0c\u542f\u52a8\u4e0b\u6e38\u4fe1\u53f7\u901a\u8def\uff0c\u6700\u7ec8\u8bf1\u5bfcI\u578b\u5e72\u6270\u7d20\u7684\u4ea7\u751f\uff0c\u5efa\u7acb\u6297\u75c5\u6bd2\u72b6\u6001\u3002<\/li>\n\n\n\n
                      • RTCB\u7684\u975e\u50ac\u5316\u89d2\u8272<\/strong>\uff1a\u7814\u7a76\u53d1\u73b0\uff0cRTCB\u5728\u6b64\u8fc7\u7a0b\u4e2d\u626e\u6f14\u4e86\u4e00\u4e2a\u8d1f\u5411\u8c03\u63a7\u56e0\u5b50\u7684\u89d2\u8272\u3002\u5b83\u901a\u8fc7\u4e0eDDX1\u76f4\u63a5\u7ade\u4e89\u6027\u7ed3\u5408\uff0c\u963b\u6b62\u4e86DDX1\u4e0eDDX21\u5f62\u6210\u5177\u6709\u4fe1\u53f7\u8f6c\u5bfc\u6d3b\u6027\u7684\u590d\u5408\u7269\u3002\u8fd9\u79cd\u5e72\u6270\u524a\u5f31\u4e86\u5bbf\u4e3b\u7684\u5148\u5929\u6027\u514d\u75ab\u5e94\u7b54\uff0c\u4ece\u800c\u4e3a\u75c5\u6bd2\u7684\u590d\u5236\u548c\u4f20\u64ad\u521b\u9020\u4e86\u6709\u5229\u6761\u4ef6 42<\/sup>\u3002<\/li>\n\n\n\n
                      • \u529f\u80fd\u8303\u5f0f\u7684\u8f6c\u53d8<\/strong>\uff1a\u8fd9\u4e00\u53d1\u73b0\u63ed\u793a\u4e86RTCB\u7684\u4e00\u79cd\u5168\u65b0\u7684\u3001\u975e\u50ac\u5316\u7684\u201c\u652f\u67b6\u201d\uff08scaffolding\uff09\u529f\u80fd\u3002\u5b83\u8868\u660eRTCB\u4e0d\u4ec5\u662f\u4e00\u4e2a\u9176\uff0c\u8fd8\u662f\u4e00\u4e2a\u80fd\u591f\u53c2\u4e0e\u6784\u5efa\u5927\u578b\u4fe1\u53f7\u8f6c\u5bfc\u5e73\u53f0\u7684\u8c03\u63a7\u86cb\u767d\u3002<\/li>\n<\/ul>\n\n\n\n

                        \u8fd9\u4e00\u53d1\u73b0\u5bf9\u4e8e\u5168\u9762\u7406\u89e3RTCB\u81f3\u5173\u91cd\u8981\u3002\u5b83\u63d0\u9192\u6211\u4eec\uff0c\u5728\u89e3\u91caRTCB\u57fa\u56e0\u6572\u9664\u6216\u6572\u4f4e\u7684\u8868\u578b\u65f6\uff0c\u4e0d\u80fd\u5148\u5165\u4e3a\u4e3b\u5730\u5c06\u5176\u5f52\u56e0\u4e8e\u8fde\u63a5\u9176\u6d3b\u6027\u7684\u4e27\u5931\u3002\u8868\u578b\u7684\u539f\u56e0\u5b8c\u5168\u6709\u53ef\u80fd\u662f\u7531\u4e8e\u67d0\u4e2a\u5173\u952e\u86cb\u767d\u8d28\u590d\u5408\u7269\u7684\u5f62\u6210\u53d7\u5230\u4e86\u7834\u574f\u3002\u56e0\u6b64\uff0c\u4e25\u8c28\u7684\u5b9e\u9a8c\u8bbe\u8ba1\uff0c\u4f8b\u5982\u4f7f\u7528\u50ac\u5316\u5931\u6d3b\u7684\u70b9\u7a81\u53d8\u4f53\u8fdb\u884c\u529f\u80fd\u56de\u8865\u5b9e\u9a8c\uff08\u6b63\u5982\u5728\u8f74\u7a81\u518d\u751f\u7814\u7a76\u4e2d\u6240\u505a\u7684\u90a3\u6837\uff09\uff0c\u5bf9\u4e8e\u533a\u5206RTCB\u7684\u50ac\u5316\u529f\u80fd\u548c\u975e\u50ac\u5316\u529f\u80fd\u662f\u5fc5\u4e0d\u53ef\u5c11\u7684\u3002\u53ea\u6709\u8fd9\u6837\uff0c\u624d\u80fd\u51c6\u786e\u5730\u89e3\u6790RTCB\u5728\u5404\u79cd\u590d\u6742\u751f\u547d\u6d3b\u52a8\u4e2d\u7684\u771f\u5b9e\u4f5c\u7528\u3002<\/p>\n\n\n\n

                        \u7b2c\u56db\u8282\uff1a\u524d\u8fdb\u4e4b\u8def\uff1a\u63ed\u793a\u5b8c\u6574RTCB\u4e92\u4f5c\u7ec4\u7684\u65b9\u6cd5\u5b66\u7b56\u7565<\/h2>\n\n\n\n

                        \u5c3d\u7ba1\u5bf9RTCB\u7684\u7814\u7a76\u5df2\u53d6\u5f97\u957f\u8db3\u8fdb\u6b65\uff0c\u4f46\u5176\u5b8c\u6574\u7684\u5e95\u7269\u8c31\uff0c\u7279\u522b\u662f\u5728\u540e\u751f\u52a8\u7269\u4e2d\uff0c\u4ecd\u7136\u662f\u4e00\u4e2a\u5de8\u5927\u7684\u672a\u77e5\u9886\u57df\u3002\u8981\u7cfb\u7edf\u6027\u5730\u9274\u5b9aRTCB\u7684\u6240\u6709RNA\u5e95\u7269\uff0c\u9700\u8981\u514b\u670d\u4e00\u7cfb\u5217\u65b9\u6cd5\u5b66\u4e0a\u7684\u6311\u6218\uff0c\u5e76\u7efc\u5408\u8fd0\u7528\u524d\u6cbf\u7684\u7ec4\u5b66\u6280\u672f\u3002<\/p>\n\n\n\n

                        4.1 \u63a2\u6d4b\u975e\u7ecf\u5178\u526a\u63a5\u7684\u6311\u6218<\/h3>\n\n\n\n

                        \u9274\u5b9a\u7531RTCB\u4ecb\u5bfc\u7684\u975e\u7ecf\u5178\u526a\u63a5\u4e8b\u4ef6\u9762\u4e34\u4e24\u5927\u4e3b\u8981\u969c\u788d\u3002<\/p>\n\n\n\n

                          \n
                        • \u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u7684\u5c40\u9650\u6027<\/strong>\uff1a\u76ee\u524d\u4e3b\u6d41\u7684RNA\u6d4b\u5e8f\uff08RNA-seq\uff09\u6570\u636e\u5206\u6790\u6d41\u7a0b\uff0c\u5176\u7b97\u6cd5\u548c\u53c2\u6570\u90fd\u662f\u4e3a\u8bc6\u522b\u7531\u526a\u63a5\u4f53\u4ecb\u5bfc\u7684\u7ecf\u5178\u526a\u63a5\u4e8b\u4ef6\u800c\u4f18\u5316\u7684\u3002\u5bf9\u4e8e\u50cfRTCB\u50ac\u5316\u7684\u8fd9\u79cd\u975e\u7ecf\u5178\u8fde\u63a5\u4e8b\u4ef6\u4ea7\u751f\u7684RNA\u8fde\u63a5\u70b9\uff0c\u5f88\u5bb9\u6613\u88ab\u6807\u51c6\u6d41\u7a0b\u8bef\u5224\u4e3a\u6d4b\u5e8f\u9519\u8bef\u3001\u57fa\u56e0\u7ec4\u91cd\u6392\u6216\u6280\u672f\u566a\u97f3\u800c\u88ab\u8fc7\u6ee4\u6389\u3002\u56e0\u6b64\uff0c\u9700\u8981\u5f00\u53d1\u4e13\u95e8\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5de5\u5177\u6765\u8bc6\u522b\u8fd9\u7c7b\u72ec\u7279\u7684\u8fde\u63a5\u6a21\u5f0f\u3002<\/li>\n\n\n\n
                        • \u5b9e\u9a8c\u5047\u8c61\u7684\u98ce\u9669<\/strong>\uff1a\u5728RNA\u7814\u7a76\u4e2d\uff0c\u4e00\u4e2a\u957f\u671f\u5b58\u5728\u7684\u9677\u9631\u662f\u7531\u9006\u8f6c\u5f55\u9176\u5728cDNA\u5408\u6210\u8fc7\u7a0b\u4e2d\u7684\u201c\u6a21\u677f\u8df3\u8dc3\u201d\uff08template switching\uff09\u6240\u4ea7\u751f\u7684\u5047\u8c61 44<\/sup>\u3002\u9006\u8f6c\u5f55\u9176\u53ef\u80fd\u4ece\u4e00\u4e2aRNA\u6a21\u677f\u4e0a\u8131\u79bb\uff0c\u7136\u540e\u4ee5\u53e6\u4e00\u4e2aRNA\u5206\u5b50\u6216\u540c\u4e00\u5206\u5b50\u7684\u4e0d\u540c\u533a\u57df\u4e3a\u6a21\u677f\u7ee7\u7eed\u5408\u6210\uff0c\u4ece\u800c\u4ea7\u751f\u5d4c\u5408\u7684cDNA\u5206\u5b50\u3002\u8fd9\u4e9b\u5206\u5b50\u5728\u6d4b\u5e8f\u540e\uff0c\u5176\u5e8f\u5217\u770b\u8d77\u6765\u6781\u50cf\u53d1\u751f\u4e86\u975e\u7ecf\u5178\u7684\u53cd\u5f0f<\/em>\u526a\u63a5\u6216\u987a\u5f0f<\/em>\u91cd\u6392\uff0c\u4e0eRTCB\u53ef\u80fd\u50ac\u5316\u7684\u4e8b\u4ef6\u96be\u4ee5\u533a\u5206\u3002\u56e0\u6b64\uff0c\u4efb\u4f55\u901a\u8fc7\u6d4b\u5e8f\u53d1\u73b0\u7684\u975e\u7ecf\u5178\u526a\u63a5\u4e8b\u4ef6\uff0c\u90fd\u5fc5\u987b\u7ecf\u8fc7\u4e25\u683c\u7684\u3001\u975e\u4f9d\u8d56\u4e8e\u9006\u8f6c\u5f55\u7684\u5b9e\u9a8c\u65b9\u6cd5\uff08\u5982Northern blot\u6216RNase\u4fdd\u62a4\u5b9e\u9a8c\uff09\u8fdb\u884c\u9a8c\u8bc1\u3002<\/li>\n<\/ul>\n\n\n\n

                          4.2 \u4ea4\u8054\u514d\u75ab\u6c89\u6dc0\u540e\u6d4b\u5e8f\uff08CLIP-seq\uff09\u7684\u529b\u91cf<\/h3>\n\n\n\n

                          \u8981\u76f4\u63a5\u3001\u7cfb\u7edf\u5730\u9274\u5b9aRTCB\u5728\u6d3b\u7ec6\u80de\u5185\u76f4\u63a5\u7ed3\u5408\u7684\u6240\u6709RNA\u5206\u5b50\uff0c\u76ee\u524d\u6700\u5f3a\u5927\u3001\u6700\u76f4\u63a5\u7684\u65b9\u6cd5\u662f\u4ea4\u8054\u514d\u75ab\u6c89\u6dc0\u540e\u6d4b\u5e8f\uff08Cross-Linking and Immunoprecipitation coupled to High-throughput Sequencing, CLIP-seq\uff09\u53ca\u5176\u9ad8\u5206\u8fa8\u7387\u7684\u884d\u751f\u6280\u672f\uff0c\u5982iCLIP\u548cPAR-CLIP 45<\/sup>\u3002<\/p>\n\n\n\n

                            \n
                          • \u6280\u672f\u539f\u7406<\/strong>\uff1aCLIP-seq\u6280\u672f\u7684\u6838\u5fc3\u6b65\u9aa4\u662f\uff0c\u9996\u5148\u4f7f\u7528\u7d2b\u5916\u7ebf\uff08UV\uff09\u5c06\u7ec6\u80de\u5185\u7684\u86cb\u767d\u8d28\u4e0e\u5176\u76f4\u63a5\u7ed3\u5408\u7684RNA\u5206\u5b50\u8fdb\u884c\u5171\u4ef7\u4ea4\u8054\uff0c\u4ece\u800c\u201c\u5b9a\u683c\u201d\u8fd9\u4e00\u77ac\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u7136\u540e\uff0c\u88c2\u89e3\u7ec6\u80de\uff0c\u4f7f\u7528\u7279\u5f02\u6027\u9488\u5bf9RTCB\u7684\u6297\u4f53\u8fdb\u884c\u514d\u75ab\u6c89\u6dc0\uff0c\u5c06RTCB\u53ca\u5176\u4ea4\u8054\u7684RNA\u7247\u6bb5\u4e00\u540c\u6355\u83b7\u3002\u7ecf\u8fc7\u4e00\u7cfb\u5217\u7eaf\u5316\u548c\u9176\u5207\u6b65\u9aa4\u540e\uff0c\u5bf9\u8fd9\u4e9b\u88abRTCB\u201c\u4fdd\u62a4\u201d\u7684RNA\u7247\u6bb5\u8fdb\u884c\u9ad8\u901a\u91cf\u6d4b\u5e8f\u3002\u6700\u7ec8\uff0c\u901a\u8fc7\u5c06\u6d4b\u5e8f\u8bfb\u6bb5\u6bd4\u5bf9\u56de\u57fa\u56e0\u7ec4\uff0c\u5c31\u53ef\u4ee5\u5728\u5168\u8f6c\u5f55\u7ec4\u8303\u56f4\u5185\uff0c\u4ee5\u63a5\u8fd1\u5355\u4e2a\u6838\u82f7\u9178\u7684\u5206\u8fa8\u7387\uff0c\u7ed8\u5236\u51faRTCB\u7684\u7cbe\u786e\u7ed3\u5408\u56fe\u8c31 45<\/sup>\u3002<\/li>\n\n\n\n
                          • \u5e94\u7528\u524d\u666f<\/strong>\uff1a\u5728\u5173\u952e\u7684\u751f\u7269\u5b66\u80cc\u666f\u4e0b\uff08\u4f8b\u5982\uff0c\u5728\u795e\u7ecf\u5143\u8f74\u7a81\u635f\u4f24\u540e\u3001\u5728\u53d1\u80b2\u4e2d\u7684\u5375\u6bcd\u7ec6\u80de\u4e2d\u3001\u6216\u5728\u7ecf\u5386\u5185\u8d28\u7f51\u80c1\u8feb\u7684\u7ec6\u80de\u4e2d\uff09\u5b9e\u65bdRTCB\u7684CLIP-seq\u5b9e\u9a8c\uff0c\u5c06\u662f\u9274\u5b9a\u7b2c\u4e8c\u8282\u4e2d\u63d0\u5230\u7684\u90a3\u4e9b\u201c\u672a\u77e5\u5e95\u7269\u201d\u7684\u51b3\u5b9a\u6027\u5b9e\u9a8c\u3002\u8fd9\u5c06\u76f4\u63a5\u56de\u7b54RTCB\u5728\u8fd9\u4e9b\u7279\u5b9a\u6761\u4ef6\u4e0b\u5230\u5e95\u7ed3\u5408\u4e86\u54ea\u4e9bRNA\u5206\u5b50\u3002<\/li>\n\n\n\n
                          • \u5f53\u524d\u9886\u57df\u7684\u7a7a\u767d<\/strong>\uff1a\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u5c3d\u7ba1CLIP-seq\u6280\u672f\u5df2\u76f8\u5f53\u6210\u719f\uff0c\u4f46\u5728\u516c\u5f00\u7684RNA-\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u6570\u636e\u5e93\uff08\u5982ENCORI\uff09\u4e2d\uff0c\u76ee\u524d\u4ecd\u7f3a\u4e4f\u5927\u89c4\u6a21\u7684\u3001\u516c\u5f00\u53ef\u7528\u7684RTCB CLIP-seq\u6570\u636e\u96c6 48<\/sup>\u3002\u8fd9\u51f8\u663e\u4e86\u8be5\u9886\u57df\u4e00\u4e2a\u4e9f\u5f85\u586b\u8865\u7684\u7a7a\u767d\uff0c\u4e5f\u9884\u793a\u7740\u8fd9\u65b9\u9762\u7684\u5de5\u4f5c\u5c06\u662f\u672a\u6765\u7814\u7a76\u7684\u4e00\u4e2a\u5173\u952e\u7a81\u7834\u53e3\u3002<\/li>\n<\/ul>\n\n\n\n

                            4.3 \u4e92\u8865\u4e0e\u66ff\u4ee3\u65b9\u6cd5<\/h3>\n\n\n\n

                            \u9664\u4e86CLIP-seq\uff0c\u8fd8\u6709\u5176\u4ed6\u4e00\u4e9b\u6280\u672f\u53ef\u4ee5\u4f5c\u4e3a\u8865\u5145\u6216\u66ff\u4ee3\u65b9\u6848\uff0c\u4ece\u4e0d\u540c\u89d2\u5ea6\u63ed\u793aRTCB\u7684RNA\u4e92\u4f5c\u7ec4\u3002<\/p>\n\n\n\n

                              \n
                            • \u57fa\u4e8e\u90bb\u8fd1\u6807\u8bb0\u7684\u65b9\u6cd5\uff08\u5982TRIBE, STAMP\uff09<\/strong>\uff1a\u8fd9\u7c7b\u6280\u672f\u901a\u8fc7\u57fa\u56e0\u5de5\u7a0b\u624b\u6bb5\uff0c\u5c06RTCB\u4e0e\u4e00\u4e2aRNA\u7f16\u8f91\u9176\uff08\u5982ADAR\u7684\u50ac\u5316\u57df\u6216APOBEC\uff09\u878d\u5408\u6210\u4e00\u4e2a\u86cb\u767d\u3002\u5f53\u8fd9\u4e2a\u878d\u5408\u86cb\u767d\u5728\u7ec6\u80de\u5185\u8868\u8fbe\u5e76\u7ed3\u5408\u5176\u9776\u6807RNA\u65f6\uff0c\u4e0e\u4e4b\u878d\u5408\u7684\u7f16\u8f91\u9176\u5c31\u4f1a\u5728\u90bb\u8fd1\u7684RNA\u5206\u5b50\u4e0a\u7559\u4e0b\u7279\u5b9a\u7684\u7f16\u8f91\u201c\u5370\u8bb0\u201d\uff08\u4f8b\u5982\uff0cA-to-I\u7684\u7f16\u8f91\uff09\u3002\u901a\u8fc7\u5bf9\u603bRNA\u8fdb\u884c\u6d4b\u5e8f\u5e76\u5bfb\u627e\u8fd9\u4e9b\u65b0\u51fa\u73b0\u7684\u7f16\u8f91\u4f4d\u70b9\uff0c\u5c31\u53ef\u4ee5\u63a8\u65ad\u51fa\u54ea\u4e9bRNA\u5206\u5b50\u66fe\u4e0eRTCB\u6709\u8fc7\u8fd1\u8ddd\u79bb\u63a5\u89e6 49<\/sup>\u3002\u8fd9\u79cd\u65b9\u6cd5\u5bf9\u4e8e\u6355\u6349\u90a3\u4e9b\u4e0eRTCB\u6709\u77ac\u65f6\u76f8\u4e92\u4f5c\u7528\u6216\u4e0d\u9002\u5408CLIP\u5b9e\u9a8c\u7684\u9776\u6807\u53ef\u80fd\u7279\u522b\u6709\u6548\u3002<\/li>\n\n\n\n
                            • \u57fa\u4e8e\u8fde\u63a5\u53cd\u5e94\u7684\u7b5b\u9009<\/strong>\uff1a\u6b63\u5982\u5728\u7ec6\u83cc\u7814\u7a76\u4e2d\u6210\u529f\u5e94\u7528\u7684\u90a3\u6837\uff0c\u53ef\u4ee5\u63d0\u53d6\u7ec6\u80de\u603bRNA\uff0c\u7528\u6838\u9178\u9176\u5c06\u5176\u968f\u673a\u5207\u5272\uff0c\u7136\u540e\u5229\u7528\u7eaf\u5316\u7684RTCB\u86cb\u767d\u548c\u4e00\u4e2a\u5e26\u6709\u7279\u6b8a\u6807\u7b7e\u7684RNA\u63a5\u5934\uff0c\u5728\u4f53\u5916\u8fdb\u884c\u8fde\u63a5\u53cd\u5e94\u3002\u53ea\u6709\u90a3\u4e9b\u5177\u6709RTCB\u53ef\u8bc6\u522b\u672b\u7aef\uff085\u2032-OH\uff09\u7684\u5185\u6e90RNA\u7247\u6bb5\u624d\u80fd\u88ab\u8fde\u63a5\u4e0a\u6807\u7b7e\u3002\u968f\u540e\uff0c\u901a\u8fc7\u5bf9\u8fd9\u4e9b\u5e26\u6807\u7b7e\u7684RNA\u8fdb\u884c\u5bcc\u96c6\u548c\u6d4b\u5e8f\uff0c\u5c31\u53ef\u4ee5\u9274\u5b9a\u51fa\u7ec6\u80de\u4e2d\u6240\u6709\u6f5c\u5728\u7684RTCB\u5e95\u7269 5<\/sup>\u3002\u8fd9\u79cd\u65b9\u6cd5\u53ef\u4ee5\u76f4\u63a5\u7b5b\u9009\u51fa\u5177\u6709\u50ac\u5316\u6f5c\u529b\u7684\u5e95\u7269\u3002<\/li>\n<\/ul>\n\n\n\n

                              \u8981\u6700\u5168\u9762\u3001\u6700\u53ef\u9760\u5730\u7ed8\u5236RTCB\u7684\u5e95\u7269\u56fe\u8c31\uff0c\u6700\u4f73\u7b56\u7565\u662f\u6574\u5408\u591a\u79cd\u65b9\u6cd5\u5b66\u7684\u6570\u636e\u3002\u6ca1\u6709\u4efb\u4f55\u4e00\u79cd\u65b9\u6cd5\u662f\u5b8c\u7f8e\u65e0\u7f3a\u7684\u3002CLIP-seq\u662f\u9274\u5b9a\u76f4\u63a5\u7ed3\u5408\u7684\u201c\u91d1\u6807\u51c6\u201d\uff0c\u4f46\u7ed3\u5408\u4e0d\u7b49\u4e8e\u50ac\u5316\u3002\u800c\u57fa\u4e8e\u57fa\u56e0\u6572\u9664\u6a21\u578b\u7684RNA-seq\uff08\u5982\u5375\u6bcd\u7ec6\u80de\u7814\u7a76\uff09\u5219\u80fd\u63ed\u793a\u54ea\u4e9b\u8f6c\u5f55\u672c\u7684\u52a0\u5de5\u8fc7\u7a0b<\/em>\u4f9d\u8d56\u4e8eRTCB\u3002\u56e0\u6b64\uff0c\u6700\u7406\u60f3\u7684\u7b56\u7565\u662f\u5c06\u8fd9\u4e9b\u6570\u636e\u8fdb\u884c\u6574\u5408\u5206\u6790\uff1a\u90a3\u4e9b\u5728CLIP-seq\u5b9e\u9a8c\u4e2d\u88ab\u8bc1\u5b9e\u4e0eRTCB\u76f4\u63a5\u7ed3\u5408\uff0c\u5e76\u4e14<\/strong>\u5728RTCB\u6572\u9664\u6a21\u578b\u7684RNA-seq\u4e2d\u8868\u73b0\u51fa\u526a\u63a5\u5f02\u5e38\u6216\u79ef\u7d2f\u7684RNA\u5206\u5b50\uff0c\u5c06\u662fRTCB\u6700\u53ef\u4fe1\u7684\u3001\u65b0\u578b\u7684\u529f\u80fd\u6027\u5e95\u7269\u3002\u8fd9\u79cd\u591a\u7ec4\u5b66\u6570\u636e\u7684\u5173\u8054\u5206\u6790\uff0c\u4e3a\u8be5\u9886\u57df\u7684\u672a\u6765\u7814\u7a76\u63d0\u4f9b\u4e86\u6700\u9ad8\u7f6e\u4fe1\u5ea6\u7684\u9274\u5b9a\u6807\u51c6\u3002<\/p>\n\n\n\n

                              \u7b2c\u4e94\u8282\uff1a\u7efc\u5408\u4e0e\u7ed3\u8bba\uff1a\u4f5c\u4e3a\u591a\u529f\u80fd\u6838\u9178\u8fde\u63a5\u9176\u7684RTCB\u65b0\u753b\u50cf<\/h2>\n\n\n\n

                              \u7efc\u5408\u73b0\u6709\u7814\u7a76\u8bc1\u636e\uff0cRNA\u8fde\u63a5\u9176RTCB\u7684\u5f62\u8c61\u5df2\u7ecf\u8fdc\u8fdc\u8d85\u51fa\u4e86\u4e00\u4e2a\u7b80\u5355\u7684tRNA\u6216XBP1\u526a\u63a5\u56e0\u5b50\u3002\u5b83\u6b63\u5728\u5c55\u73b0\u4e3a\u4e00\u4e2a\u591a\u529f\u80fd\u7684\u6838\u9178\u8fde\u63a5\u9176\u548c\u8c03\u63a7\u86cb\u767d\uff0c\u4f4d\u4e8e\u7ec6\u80de\u8d28\u91cf\u63a7\u5236\u3001\u80c1\u8feb\u5e94\u7b54\u548c\u53d1\u80b2\u8c03\u63a7\u7f51\u7edc\u7684\u6838\u5fc3\u3002\u672c\u62a5\u544a\u65e8\u5728\u7cfb\u7edf\u6027\u5730\u56de\u7b54\u201c\u9664\u4e86XBP1\u548ctRNA\uff0cRTCB\u8fd8\u8d1f\u8d23\u54ea\u4e9bRNA\u7684\u975e\u7ecf\u5178\u526a\u63a5\u201d\u8fd9\u4e00\u95ee\u9898\uff0c\u5e76\u63cf\u7ed8\u51fa\u8be5\u9886\u57df\u7684\u524d\u6cbf\u548c\u672a\u6765\u65b9\u5411\u3002<\/p>\n\n\n\n

                              5.1 \u7814\u7a76\u53d1\u73b0\u56de\u987e\uff1a\u8d85\u8d8atRNA\u4e0eXBP1<\/h3>\n\n\n\n

                              \u5c3d\u7ba1\u5728\u540e\u751f\u52a8\u7269\u4e2d\uff0c\u542b\u6709\u5185\u542b\u5b50\u7684tRNA\u548cXBP1 mRNA\u662fRTCB\u4ecb\u5bfc\u7684\u8fde\u63a5\u53cd\u5e94\u4ec5\u6709\u7684\u3001\u88ab\u666e\u904d\u786e\u8ba4\u7684\u5e95\u7269\uff0c\u4f46\u5927\u91cf\u4e14\u591a\u65b9\u9762\u7684\u8bc1\u636e\u5f3a\u70c8\u8868\u660e\uff0c\u5176\u5e95\u7269\u8303\u56f4\u8981\u5e7f\u6cdb\u5f97\u591a\u3002<\/p>\n\n\n\n

                                \n
                              • \u5728\u539f\u6838\u751f\u7269<\/strong>\u4e2d\uff0cRTCB\u4f5c\u4e3aRNA\u4fee\u590d\u7cfb\u7edf\u7684\u6838\u5fc3\uff0c\u8d1f\u8d23\u8fde\u63a5\u5728\u80c1\u8feb\u4e0b\u65ad\u88c2\u7684\u6838\u7cd6\u4f53RNA\uff08rRNA\uff09\u548c\u8f6c\u79fb-\u4fe1\u4f7fRNA\uff08tmRNA\uff09<\/strong>\uff0c\u4ee5\u7ef4\u6301\u7ffb\u8bd1\u673a\u5668\u7684\u5b8c\u6574\u6027\u548c\u6548\u7387 5<\/sup>\u3002<\/li>\n\n\n\n
                              • \u5728\u5c0f\u9f20<\/strong>\u7684\u53d1\u80b2\u8fc7\u7a0b\u4e2d\uff0cRTCB\u901a\u8fc7\u8c03\u63a7\u4e00\u7c7b\u7279\u6b8a\u7684\u5185\u542b\u5b50\u4fdd\u7559mRNA<\/strong>\u7684\u526a\u63a5\uff0c\u786e\u4fdd\u4e86\u5375\u6bcd\u7ec6\u80de\u7684\u6b63\u5e38\u6210\u719f\u3002\u8fd9\u4e9bmRNA\u4e3b\u8981\u7f16\u7801\u53c2\u4e0eDNA\u635f\u4f24\u5e94\u7b54<\/strong>\u548cDNA\u7532\u57fa\u5316<\/strong>\u7684\u5173\u952e\u86cb\u767d\uff0c\u63ed\u793a\u4e86RTCB\u5728\u7ef4\u6301\u57fa\u56e0\u7ec4\u7a33\u5b9a\u6027\u548c\u8868\u89c2\u9057\u4f20\u8c03\u63a7\u4e2d\u7684\u65b0\u4f5c\u7528 5<\/sup>\u3002<\/li>\n\n\n\n
                              • \u5728\u79c0\u4e3d\u9690\u6746\u7ebf\u866b<\/strong>\u7684\u795e\u7ecf\u7cfb\u7edf\u4e2d\uff0cRTCB\u7684\u8fde\u63a5\u9176\u6d3b\u6027\u88ab\u8bc1\u5b9e\u53c2\u4e0e\u8fde\u63a5\u4e00\u79cd\u672a\u77e5\u7684RNA\u6291\u5236\u56e0\u5b50<\/strong>\uff0c\u4ece\u800c\u6291\u5236\u8f74\u7a81\u635f\u4f24\u540e\u7684\u518d\u751f\u3002\u8fd9\u4e00\u529f\u80fd\u4e0e\u5df2\u77e5\u7684UPR\u548ctRNA\u901a\u8def\u5b8c\u5168\u72ec\u7acb\uff0c\u4e3aRTCB\u5b58\u5728\u65b0\u5e95\u7269\u63d0\u4f9b\u4e86\u51b3\u5b9a\u6027\u7684\u9057\u4f20\u5b66\u8bc1\u636e 2<\/sup>\u3002<\/li>\n\n\n\n
                              • \u6b64\u5916\uff0cRTCB\u8fd8\u5c55\u73b0\u51fa\u8d85\u8d8aRNA\u8fde\u63a5\u7684\u975e\u5e38\u89c4\u6d3b\u6027<\/strong>\uff0c\u5305\u62ec\u5728\u7ec6\u83cc\u4e2d\u53d1\u73b0\u7684DNA\u672b\u7aef\u52a0\u5e3d<\/strong>\u529f\u80fd\uff0c\u4ee5\u53ca\u5728\u75c5\u6bd2\u611f\u67d3\u4e2d\u4e0d\u4f9d\u8d56\u50ac\u5316\u6d3b\u6027\u7684\u514d\u75ab\u6291\u5236\u652f\u67b6<\/strong>\u529f\u80fd 5<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n

                                5.2 RTCB\u5e95\u7269\u4e0e\u6d3b\u6027\u603b\u7ed3<\/h3>\n\n\n\n

                                \u4e3a\u4e86\u6e05\u6670\u5730\u6982\u62ecRTCB\u7684\u529f\u80fd\u591a\u6837\u6027\uff0c\u4e0b\u8868\u7cfb\u7edf\u6027\u5730\u603b\u7ed3\u4e86\u5176\u5df2\u77e5\u7684\u548c\u6f5c\u5728\u7684\u5e95\u7269\u3001\u76f8\u5173\u751f\u7269\u5b66\u8fc7\u7a0b\u3001\u8bc1\u636e\u5f3a\u5ea6\u4ee5\u53ca\u5173\u952e\u7684\u7814\u7a76\u6765\u6e90\u3002<\/p>\n\n\n\n

                                \u6a21\u5f0f\u751f\u7269<\/td>\u5e95\u7269 \/ \u6d3b\u6027<\/td>\u751f\u7269\u5b66\u8fc7\u7a0b<\/td>\u8bc1\u636e\u5f3a\u5ea6<\/td>\u5173\u952e\u7814\u7a76\u6765\u6e90<\/td><\/tr><\/thead>
                                \u540e\u751f\u52a8\u7269<\/td>\u542b\u5185\u542b\u5b50\u7684tRNA<\/td>tRNA\u6210\u719f\uff0c\u86cb\u767d\u8d28\u5408\u6210<\/td>\u51b3\u5b9a\u6027<\/strong><\/td>2<\/sup><\/td><\/tr>
                                \u540e\u751f\u52a8\u7269<\/td>XBP1 mRNA<\/td>\u672a\u6298\u53e0\u86cb\u767d\u53cd\u5e94 (UPR)<\/td>\u51b3\u5b9a\u6027<\/strong><\/td>2<\/sup><\/td><\/tr>
                                \u7ec6\u83cc<\/td>\u6838\u7cd6\u4f53RNA (rRNA)<\/td>RNA\u4fee\u590d\uff0c\u80c1\u8feb\u6062\u590d<\/td>\u5f3a (\u76f4\u63a5\u8bc1\u636e)<\/strong><\/td>5<\/sup><\/td><\/tr>
                                \u5c0f\u9f20<\/td>\u5185\u542b\u5b50\u4fdd\u7559\u7684mRNA (DDR, \u7532\u57fa\u5316\u57fa\u56e0)<\/td>\u5375\u6bcd\u7ec6\u80de\u6210\u719f\uff0c\u9009\u62e9\u6027\u526a\u63a5\u8c03\u63a7<\/td>\u5f3a (\u9057\u4f20\u5b66\u8bc1\u636e)<\/strong><\/td>5<\/sup><\/td><\/tr>
                                \u79c0\u4e3d\u9690\u6746\u7ebf\u866b<\/td>\u672a\u77e5RNA(s)<\/td>\u6291\u5236\u8f74\u7a81\u518d\u751f<\/td>\u5f3a (\u9057\u4f20\u5b66\u8bc1\u636e)<\/strong><\/td>2<\/sup><\/td><\/tr>
                                \u7ec6\u83cc<\/td>tmRNA (ssrA<\/em>)<\/td>\u6838\u7cd6\u4f53\u62ef\u6551\uff0cRNA\u4fee\u590d<\/td>\u4e2d\u7b49 (\u63a8\u65ad)<\/strong><\/td>5<\/sup><\/td><\/tr>
                                \u7ec6\u83cc<\/td>DNA (3′-P\u672b\u7aef)<\/td>DNA\u672b\u7aef\u52a0\u5e3d\uff0cDNA\u635f\u4f24\u5e94\u7b54<\/td>\u4e2d\u7b49 (\u751f\u5316\u8bc1\u636e)<\/strong><\/td>5<\/sup><\/td><\/tr>
                                \u771f\u6838\u751f\u7269<\/td>\u5176\u4ed6ncRNA (lncRNA, miRNA)<\/td>\u63a8\u6d4b\u6027<\/td>\u63a8\u6d4b\u6027<\/strong><\/td>5<\/sup><\/td><\/tr>
                                \u771f\u6838\u751f\u7269<\/td>\u652f\u67b6\u86cb\u767d (\u4e0eDDX1\u4e92\u4f5c)<\/td>\u5148\u5929\u6027\u514d\u75ab (\u975e\u8fde\u63a5\u9176\u529f\u80fd)<\/td>\u5f3a (\u751f\u5316\u8bc1\u636e)<\/strong><\/td>42<\/sup><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

                                5.3 \u6700\u7ec8\u7efc\u5408\u4e0e\u672a\u6765\u5c55\u671b<\/h3>\n\n\n\n

                                \u7efc\u5408\u6240\u6709\u8bc1\u636e\uff0cRTCB\u7684\u753b\u50cf\u4e0d\u518d\u662f\u4e00\u4e2a\u529f\u80fd\u5355\u4e00\u7684\u526a\u63a5\u56e0\u5b50\uff0c\u800c\u662f\u4e00\u4e2a\u591a\u624d\u591a\u827a\u7684\u6838\u9178\u8fde\u63a5\u9176\u548c\u8c03\u63a7\u86cb\u767d\u3002\u5b83\u7684\u529f\u80fd\u7f51\u7edc\u6a2a\u8de8\u4e86RNA\u6210\u719f\u3001RNA\u4fee\u590d\u3001\u80c1\u8feb\u5e94\u7b54\u3001\u53d1\u80b2\u8c03\u63a7\u3001\u57fa\u56e0\u7ec4\u7a33\u5b9a\u4e43\u81f3\u975e\u50ac\u5316\u7684\u514d\u75ab\u8c03\u63a7\u7b49\u591a\u4e2a\u6838\u5fc3\u751f\u547d\u9886\u57df\u3002\u5176\u72ec\u7279\u7684\u50ac\u5316\u673a\u5236\u4f7f\u5176\u80fd\u591f\u5904\u7406\u7531\u7279\u5b9a\u4fe1\u53f7\u6216\u635f\u4f24\u4ea7\u751f\u7684\u975e\u7ecf\u5178RNA\u672b\u7aef\uff0c\u6210\u4e3a\u7ec6\u80de\u5e94\u5bf9\u5185\u5916\u73af\u5883\u53d8\u5316\u7684\u5feb\u901f\u53cd\u5e94\u7cfb\u7edf\u4e2d\u7684\u5173\u952e\u4e00\u73af\u3002<\/p>\n\n\n\n

                                \u5f53\u524d\uff0c\u8be5\u9886\u57df\u6700\u6838\u5fc3\u7684\u672a\u89e3\u4e4b\u8c1c\u4ecd\u7136\u662f\u7cfb\u7edf\u6027\u5730\u9274\u5b9aRTCB\u5728\u540e\u751f\u52a8\u7269\u4e2d\u7684\u5b8c\u6574\u5e95\u7269\u8c31\u3002\u5728\u8f74\u7a81\u518d\u751f\u4e2d\u8d77\u4f5c\u7528\u7684\u201c\u672a\u77e5\u6291\u5236\u56e0\u5b50\u201d\u548c\u5728\u5375\u6bcd\u7ec6\u80de\u4e2d\u53d7\u8c03\u63a7\u7684\u201c\u5185\u542b\u5b50\u4fdd\u7559mRNA\u201d\uff0c\u662f\u5f53\u524d\u6700\u6fc0\u52a8\u4eba\u5fc3\u3001\u6700\u553e\u624b\u53ef\u5f97\u7684\u53d1\u73b0\u76ee\u6807\u3002<\/p>\n\n\n\n

                                \u5c55\u671b\u672a\u6765\uff0c\u901a\u8fc7\u7efc\u5408\u8fd0\u7528\u73b0\u4ee3\u8f6c\u5f55\u7ec4\u5b66\u6280\u672f\uff0c\u7279\u522b\u662f\u5c06CLIP-seq\u4e0e\u57fa\u56e0\u6572\u9664\/\u7a81\u53d8\u6a21\u578b\u7684RNA-seq\u6570\u636e\u76f8\u7ed3\u5408\uff0c\u6211\u4eec\u6709\u671b\u5728\u4e0d\u4e45\u7684\u5c06\u6765\u5b8c\u5168\u63ed\u793aRTCB\u7684RNA\u4e92\u4f5c\u7f51\u7edc\u3002\u6b64\u5916\uff0c\u9a8c\u8bc1\u5176DNA\u52a0\u5e3d\u6d3b\u6027\u662f\u5426\u5728\u771f\u6838\u751f\u7269\u4e2d\u4fdd\u5b88\uff0c\u4ee5\u53ca\u6df1\u5165\u89e3\u6790\u5176\u975e\u50ac\u5316\u652f\u67b6\u529f\u80fd\u7684\u5206\u5b50\u7ec6\u8282\uff0c\u4e5f\u5c06\u662f\u91cd\u8981\u7684\u7814\u7a76\u65b9\u5411\u3002\u8fd9\u4e9b\u5de5\u4f5c\u5c06\u5f7b\u5e95\u6539\u53d8\u6211\u4eec\u5bf9\u8fd9\u4e00\u72ec\u7279\u800c\u91cd\u8981\u7684\u9176\u7684\u7406\u89e3\uff0c\u5e76\u53ef\u80fd\u4e3a\u76f8\u5173\u75be\u75c5\uff08\u5982\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\u3001\u4e0d\u5b55\u4e0d\u80b2\u548c\u764c\u75c7\uff09\u7684\u6cbb\u7597\u63d0\u4f9b\u65b0\u7684\u9776\u70b9\u548c\u7b56\u7565\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

                                \u8fd9\u7bc7\u7531Deep Research\u751f\u6210\u7684\u7efc\u8ff0\uff0c\u7cfb\u7edf\u68b3\u7406\u4e86RNA\u8fde\u63a5\u9176RTCB\u8d85\u8d8a\u5176\u7ecf\u5178tRNA\u4e0eXBP1\u526a\u63a5\u529f\u80fd\u7684\u6700\u65b0\u8fdb\u5c55\u3002\u6587\u7ae0\u8be6\u5c3d\u5206\u6790\u4e86\u5176\u5728\u7ec6\u83ccRNA\u4fee\u590d\u3001\u54fa\u4e73\u52a8\u7269\u53d1\u80b2\u8c03\u63a7\u53ca\u795e\u7ecf\u5143\u518d\u751f\u6291\u5236\u4e2d\u7684\u65b0\u89d2\u8272\uff0c\u5e76\u63a2\u8ba8\u4e86\u5176\u6f5c\u5728\u7684DNA\u4fee\u9970\u4e0e\u975e\u50ac\u5316\u652f\u67b6\u529f\u80fd\uff0c\u4e3a\u7406\u89e3\u8fd9\u4e00\u591a\u529f\u80fd\u6838\u9178\u8fde\u63a5\u9176\u7684\u5b8c\u6574\u751f\u7269\u5b66\u56fe\u666f\u63d0\u4f9b\u4e86\u524d\u6cbf\u89c6\u89d2\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[32],"class_list":["post-10691","post","type-post","status-publish","format-standard","hentry","category-18","tag-deep-research"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/10691","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10691"}],"version-history":[{"count":3,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/10691\/revisions"}],"predecessor-version":[{"id":13832,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/10691\/revisions\/13832"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10691"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10691"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10691"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}