{"id":11369,"date":"2025-08-29T04:12:31","date_gmt":"2025-08-28T20:12:31","guid":{"rendered":"https:\/\/fanyiming.life\/?p=11369"},"modified":"2025-12-21T01:02:58","modified_gmt":"2025-12-20T17:02:58","slug":"%f0%9f%93%9d-%e6%98%be%e5%be%ae%e5%9b%be%e5%a4%84%e7%90%86%e6%b5%81%e7%a8%8b%ef%bc%88%e6%9e%81%e7%ae%80%e7%89%88%ef%bc%89","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=11369","title":{"rendered":"ImageJ\u514d\u75ab\u8367\u5149\u56fe\u7247\u5904\u7406"},"content":{"rendered":"\n<p>\ud83d\udcdd \u663e\u5fae\u56fe\u5904\u7406\u6d41\u7a0b\uff08\u6781\u7b80\u7248\uff09<\/p>\n\n\n\n<p>\u62d6\u5165 3 \u4e2a\u89c6\u91ce\u5230 ImageJ<br>\u6559\u5b66\uff1a\u76f4\u63a5\u62d6\u62fd\u6587\u4ef6\u5373\u53ef\u5bfc\u5165\uff0c\u6bcf\u4e2a\u56fe\u50cf\u542b 2 \u901a\u9053\uff08C=0 DAPI\uff0cC=1 GFP\uff09\u3002<\/p>\n\n\n\n<p>\u5728 Stack \u65f6\u9009\u62e9 C=0\uff08DAPI\uff09\u3001C=1\uff08GFP\uff09<br>\u6559\u5b66\uff1a\u4fdd\u8bc1\u751f\u6210\u7684 stack \u5e26\u4e0a\u4e24\u4e2a\u901a\u9053\uff0c\u65b9\u4fbf\u540e\u7eed\u64cd\u4f5c\u3002<\/p>\n\n\n\n<p>\u52a0 Scale Bar<br>\u6559\u5b66\uff1a\u6bd4\u4f8b\u5c3a\u662f overlay\uff0c\u5e2e\u52a9\u5c55\u793a\u5b9e\u9645\u5c3a\u5bf8\u3002<\/p>\n\n\n\n<p>Flatten \u5e76\u4fdd\u5b58\u5f69\u8272\u56fe<br>\u6559\u5b66\uff1aFlatten \u4f1a\u628a\u989c\u8272\u548c\u6bd4\u4f8b\u5c3a\u70e4\u8fdb\u50cf\u7d20\uff0c\u751f\u6210 RGB \u5f69\u56fe\uff0c\u6570\u636e\u4e0d\u53ef\u518d\u62c6\u3002<\/p>\n\n\n\n<p>Concatenate \u62fc\u63a5\u591a\u4e2a stack<br>\u6559\u5b66\uff1aConcatenate \u628a\u591a\u4e2a stack \u9996\u5c3e\u76f8\u8fde\uff0c\u5f97\u5230\u4e00\u4e2a\u957f stack\u3002<\/p>\n\n\n\n<p>Make Montage<br>\u6559\u5b66\uff1aMontage \u628a stack \u5c55\u5f00\u4e3a\u62fc\u8d34\u56fe\uff0c\u65b9\u4fbf\u5c55\u793a\u3002<\/p>\n\n\n\n<p>\u4fdd\u5b58\u7ed3\u679c\u56fe<br>\u6559\u5b66\uff1a\u4fdd\u5b58\u4e3a TIFF\/PNG\uff0c\u4fdd\u8bc1\u989c\u8272\u56fa\u5b9a\u3002<\/p>\n\n\n\n<p>\u7528 Photoshop \u5408\u5e76\u4e0d\u540c\u5904\u7406\u7ec4<br>\u6559\u5b66\uff1aPS \u7528\u4e8e\u6700\u540e\u7684\u81ea\u7531\u6392\u7248\u548c\u6807\u6ce8\uff0c\u9002\u5408\u6295\u7a3f\u56fe\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n\n\n\n\n<figure class=\"wp-block-video\"><video height=\"1080\" style=\"aspect-ratio: 1920 \/ 1080;\" width=\"1920\" controls src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/08\/20250829043221925.mp4\"><\/video><\/figure>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p>Macro\u811a\u672c\u662f\u4e2a\u597d\u4e1c\u897f\u554a\uff1a<\/p>\n\n\n\n<div class=\"wp-block-kevinbatdorf-code-block-pro cbp-has-line-numbers\" data-code-block-pro-font-family=\"Code-Pro-JetBrains-Mono\" style=\"font-size:.875rem;font-family:Code-Pro-JetBrains-Mono,ui-monospace,SFMono-Regular,Menlo,Monaco,Consolas,monospace;--cbp-line-number-color:#d8dee9ff;--cbp-line-number-width:calc(3 * 0.6 * .875rem);line-height:1.25rem;--cbp-tab-width:2;tab-size:var(--cbp-tab-width, 2)\"><span style=\"display:flex;align-items:center;padding:10px 0px 10px 16px;margin-bottom:-2px;width:100%;text-align:left;background-color:#39404f;color:#c8d0e0\">JavaScript<\/span><span role=\"button\" tabindex=\"0\" style=\"color:#d8dee9ff;display:none\" aria-label=\"\u590d\u5236\" class=\"code-block-pro-copy-button\"><pre class=\"code-block-pro-copy-button-pre\" aria-hidden=\"true\"><textarea class=\"code-block-pro-copy-button-textarea\" tabindex=\"-1\" aria-hidden=\"true\" readonly>\/\/ =========================================================\n\/\/   \u5168\u81ea\u52a8\u6279\u91cf\u62fc\u56fe\u5b8f (\u5f00\u542f Autoscale \u7248)\n\/\/   \u529f\u80fd\uff1a\u904d\u5386\u6587\u4ef6\u5939 -> Bio-Formats\u5bfc\u5165 -> \u81ea\u52a8\u8c03\u6574\u4eae\u5ea6 -> \u62fc\u56fe -> \u4fdd\u5b58\n\/\/ =========================================================\n\n\/\/ --- 1. \u5f39\u51fa\u7a97\u53e3\u9009\u62e9\u6587\u4ef6\u5939 ---\ninputDir = getDirectory(\"\u8bf7\u9009\u62e9\u5305\u542b .nd2 \u6587\u4ef6\u7684 \u8f93\u5165 \u6587\u4ef6\u5939\");\noutputDir = getDirectory(\"\u8bf7\u9009\u62e9\u4fdd\u5b58\u7ed3\u679c\u56fe\u7247\u7684 \u8f93\u51fa \u6587\u4ef6\u5939\");\n\n\/\/ \u83b7\u53d6\u6587\u4ef6\u5939\u5185\u6240\u6709\u6587\u4ef6\u5217\u8868\nlist = getFileList(inputDir);\n\n\/\/ \u5f00\u542f\u201c\u6279\u5904\u7406\u6a21\u5f0f\u201d\uff0c\u540e\u53f0\u8fd0\u884c\u4e0d\u5f39\u7a97\uff0c\u901f\u5ea6\u98de\u5feb\nsetBatchMode(true); \n\n\/\/ --- 2. \u5f00\u59cb\u5faa\u73af\u904d\u5386\u6587\u4ef6 ---\nfor (i = 0; i &lt; list.length; i++) {\n    filename = list&#091;i&#093;;\n    \n    \/\/ \u53ea\u5904\u7406 .nd2 \u7ed3\u5c3e\u7684\u6587\u4ef6\n    if (endsWith(filename, \".nd2\")) {\n        \n        \/\/ --- A. \u6253\u5f00\u56fe\u7247 ---\n        path = inputDir + filename;\n        run(\"Bio-Formats Importer\", \"open=&#091;\" + path + \"&#093; color_mode=Default view=Hyperstack stack_order=XYCZT\");\n        \n        \/\/ \u83b7\u53d6\u57fa\u672c\u4fe1\u606f\n        nameWithoutExt = File.nameWithoutExtension; \n        title = getTitle();\n        \n        \/\/ --- B. \u5904\u7406\u903b\u8f91 ---\n        \n        \/\/ \u62c6\u5206\u901a\u9053\n        run(\"Split Channels\");\n        \n        \/\/ \u5173\u95ed\u4e0d\u9700\u8981\u7684\u660e\u573a (\u5047\u8bbe\u662f C3)\n        if (isOpen(\"C3-\" + title)) {\n            selectWindow(\"C3-\" + title);\n            close();\n        }\n\n        \/\/ --- \u5904\u7406 DAPI (C1) ---\n        selectWindow(\"C1-\" + title);\n        run(\"Blue\"); \n        \/\/ \u3010Autoscale\u3011\u81ea\u52a8\u589e\u5f3a\u5bf9\u6bd4\u5ea6 (0.35% \u9971\u548c\u5ea6)\n        run(\"Enhance Contrast\", \"saturated=0.35\"); \n        \n        \/\/ \u590d\u5236\u4e00\u4efd\u7528\u4e8e Merge\n        run(\"Duplicate...\", \"title=DAPI_For_Merge\");\n        \n        \/\/ \u5236\u4f5c\u5355\u5f20 DAPI \u56fe\n        selectWindow(\"C1-\" + title);\n        run(\"Flatten\"); \n        rename(\"Final_DAPI\");\n\n        \/\/ --- \u5904\u7406 HA-RTCB (C2) ---\n        selectWindow(\"C2-\" + title);\n        run(\"Red\"); \n        \n        \/\/ \u3010Autoscale - \u4fee\u6539\u5904\u3011\u8fd9\u91cc\u4e0d\u518d\u4f7f\u7528 setMinAndMax \u56fa\u5b9a\u503c\n        \/\/ \u800c\u662f\u4f7f\u7528\u81ea\u52a8\u589e\u5f3a\uff0c\u8ba9\u6bcf\u5f20\u56fe\u90fd\u6e05\u6670\u663e\u793a\n        run(\"Enhance Contrast\", \"saturated=0.35\"); \n        \n        \/\/ \u590d\u5236\u4e00\u4efd\u7528\u4e8e Merge\n        run(\"Duplicate...\", \"title=HA_For_Merge\");\n        \n        \/\/ \u5236\u4f5c\u5355\u5f20 HA \u56fe\n        selectWindow(\"C2-\" + title);\n        run(\"Flatten\"); \n        rename(\"Final_HA\");\n\n        \/\/ --- \u5236\u4f5c Merge \u56fe ---\n        \/\/ \u4f7f\u7528\u521a\u624d\u5df2\u7ecf\u88ab Autoscale \u8fc7\u7684\u56fe\u6765\u5408\u5e76\uff0c\u6240\u4ee5 Merge \u56fe\u4e5f\u4f1a\u5f88\u6f02\u4eae\n        run(\"Merge Channels...\", \"c1=DAPI_For_Merge c2=HA_For_Merge create\");\n        selectWindow(\"Composite\");\n        run(\"Flatten\"); \n        rename(\"Final_Merge\");\n\n        \/\/ --- \u62fc\u63a5\u4e09\u5f20 RGB \u56fe ---\n        \/\/ \u987a\u5e8f\uff1aDAPI -> HA -> Merge\n        run(\"Concatenate...\", \"  title=&#091;Montage_Stack&#093; image1=Final_DAPI image2=Final_HA image3=Final_Merge image4=&#091;-- None --&#093;\");\n\n        \/\/ --- \u5236\u4f5c\u62fc\u56fe (Montage) ---\n        selectWindow(\"Montage_Stack\");\n        \/\/ border=5 \u662f\u56fe\u7247\u4e4b\u95f4\u7684\u767d\u8272\u95f4\u9699\n        run(\"Make Montage...\", \"columns=3 rows=1 scale=1 border=5\");\n\n        \/\/ --- \u52a0\u6bd4\u4f8b\u5c3a ---\n        run(\"Scale Bar...\", \"width=10 height=8 font=12 color=White background=None location=&#091;Lower Right&#093; bold overlay\");\n\n        \/\/ --- Flatten \u6700\u7ec8\u7ed3\u679c ---\n        run(\"Flatten\");\n        \n        \/\/ --- C. \u4fdd\u5b58\u7ed3\u679c ---\n        savePath = outputDir + nameWithoutExt + \"_Panel.png\";\n        saveAs(\"PNG\", savePath);\n        \n        \/\/ --- D. \u6e05\u7406\u5185\u5b58 ---\n        close(\"*\");\n        \n        \/\/ \u6253\u5370\u8fdb\u5ea6\n        print(\"\u5df2\u5904\u7406: \" + filename);\n    }\n}\n\nsetBatchMode(false);\nshowMessage(\"Autoscale \u5904\u7406\u6a21\u5f0f\uff1a\u6240\u6709\u56fe\u7247\u5df2\u5904\u7406\u5b8c\u6bd5\uff01\");<\/textarea><\/pre><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" style=\"width:24px;height:24px\" fill=\"none\" viewBox=\"0 0 24 24\" stroke=\"currentColor\" stroke-width=\"2\"><path class=\"with-check\" stroke-linecap=\"round\" stroke-linejoin=\"round\" d=\"M9 5H7a2 2 0 00-2 2v12a2 2 0 002 2h10a2 2 0 002-2V7a2 2 0 00-2-2h-2M9 5a2 2 0 002 2h2a2 2 0 002-2M9 5a2 2 0 012-2h2a2 2 0 012 2m-6 9l2 2 4-4\"><\/path><path class=\"without-check\" stroke-linecap=\"round\" stroke-linejoin=\"round\" d=\"M9 5H7a2 2 0 00-2 2v12a2 2 0 002 2h10a2 2 0 002-2V7a2 2 0 00-2-2h-2M9 5a2 2 0 002 2h2a2 2 0 002-2M9 5a2 2 0 012-2h2a2 2 0 012 2\"><\/path><\/svg><\/span><pre class=\"shiki nord\" style=\"background-color: #2e3440ff\" tabindex=\"0\"><code><span class=\"line\"><span style=\"color: #616E88\">\/\/ =========================================================<\/span><\/span>\n<span class=\"line\"><span style=\"color: #616E88\">\/\/   \u5168\u81ea\u52a8\u6279\u91cf\u62fc\u56fe\u5b8f (\u5f00\u542f Autoscale \u7248)<\/span><\/span>\n<span class=\"line\"><span style=\"color: #616E88\">\/\/   \u529f\u80fd\uff1a\u904d\u5386\u6587\u4ef6\u5939 -&gt; Bio-Formats\u5bfc\u5165 -&gt; \u81ea\u52a8\u8c03\u6574\u4eae\u5ea6 -&gt; \u62fc\u56fe -&gt; \u4fdd\u5b58<\/span><\/span>\n<span class=\"line\"><span style=\"color: #616E88\">\/\/ =========================================================<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #616E88\">\/\/ --- 1. \u5f39\u51fa\u7a97\u53e3\u9009\u62e9\u6587\u4ef6\u5939 ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">inputDir<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">=<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #88C0D0\">getDirectory<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">\u8bf7\u9009\u62e9\u5305\u542b .nd2 \u6587\u4ef6\u7684 \u8f93\u5165 \u6587\u4ef6\u5939<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">outputDir<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">=<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #88C0D0\">getDirectory<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">\u8bf7\u9009\u62e9\u4fdd\u5b58\u7ed3\u679c\u56fe\u7247\u7684 \u8f93\u51fa \u6587\u4ef6\u5939<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #616E88\">\/\/ \u83b7\u53d6\u6587\u4ef6\u5939\u5185\u6240\u6709\u6587\u4ef6\u5217\u8868<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">list<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">=<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #88C0D0\">getFileList<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #D8DEE9\">inputDir<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #616E88\">\/\/ \u5f00\u542f\u201c\u6279\u5904\u7406\u6a21\u5f0f\u201d\uff0c\u540e\u53f0\u8fd0\u884c\u4e0d\u5f39\u7a97\uff0c\u901f\u5ea6\u98de\u5feb<\/span><\/span>\n<span class=\"line\"><span style=\"color: #88C0D0\">setBatchMode<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #81A1C1\">true<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #616E88\">\/\/ --- 2. \u5f00\u59cb\u5faa\u73af\u904d\u5386\u6587\u4ef6 ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #81A1C1\">for<\/span><span style=\"color: #D8DEE9FF\"> (<\/span><span style=\"color: #D8DEE9\">i<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">=<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #B48EAD\">0<\/span><span style=\"color: #81A1C1\">;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">i<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">&lt;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">list<\/span><span style=\"color: #ECEFF4\">.<\/span><span style=\"color: #D8DEE9FF\">length<\/span><span style=\"color: #81A1C1\">;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">i<\/span><span style=\"color: #81A1C1\">++<\/span><span style=\"color: #D8DEE9FF\">) <\/span><span style=\"color: #ECEFF4\">{<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">    <\/span><span style=\"color: #D8DEE9\">filename<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">=<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">list<\/span><span style=\"color: #D8DEE9FF\">&#091;<\/span><span style=\"color: #D8DEE9\">i<\/span><span style=\"color: #D8DEE9FF\">&#093;<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">    <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">    <\/span><span style=\"color: #616E88\">\/\/ \u53ea\u5904\u7406 .nd2 \u7ed3\u5c3e\u7684\u6587\u4ef6<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">    <\/span><span style=\"color: #81A1C1\">if<\/span><span style=\"color: #D8DEE9FF\"> (<\/span><span style=\"color: #88C0D0\">endsWith<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #D8DEE9\">filename<\/span><span style=\"color: #ECEFF4\">,<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">.nd2<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)) <\/span><span style=\"color: #ECEFF4\">{<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ --- A. \u6253\u5f00\u56fe\u7247 ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #D8DEE9\">path<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">=<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">inputDir<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">filename<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Bio-Formats Importer<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #ECEFF4\">,<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">open=&#091;<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">path<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">&#093; color_mode=Default view=Hyperstack stack_order=XYCZT<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u83b7\u53d6\u57fa\u672c\u4fe1\u606f<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #D8DEE9\">nameWithoutExt<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">=<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">File<\/span><span style=\"color: #ECEFF4\">.<\/span><span style=\"color: #D8DEE9\">nameWithoutExtension<\/span><span style=\"color: #81A1C1\">;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #D8DEE9\">title<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">=<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #88C0D0\">getTitle<\/span><span style=\"color: #D8DEE9FF\">()<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ --- B. \u5904\u7406\u903b\u8f91 ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u62c6\u5206\u901a\u9053<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Split Channels<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u5173\u95ed\u4e0d\u9700\u8981\u7684\u660e\u573a (\u5047\u8bbe\u662f C3)<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #81A1C1\">if<\/span><span style=\"color: #D8DEE9FF\"> (<\/span><span style=\"color: #88C0D0\">isOpen<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">C3-<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">title<\/span><span style=\"color: #D8DEE9FF\">)) <\/span><span style=\"color: #ECEFF4\">{<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">            <\/span><span style=\"color: #88C0D0\">selectWindow<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">C3-<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">title<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">            <\/span><span style=\"color: #88C0D0\">close<\/span><span style=\"color: #D8DEE9FF\">()<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #ECEFF4\">}<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ --- \u5904\u7406 DAPI (C1) ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">selectWindow<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">C1-<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">title<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Blue<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u3010Autoscale\u3011\u81ea\u52a8\u589e\u5f3a\u5bf9\u6bd4\u5ea6 (0.35% \u9971\u548c\u5ea6)<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Enhance Contrast<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #ECEFF4\">,<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">saturated=0.35<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u590d\u5236\u4e00\u4efd\u7528\u4e8e Merge<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Duplicate...<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #ECEFF4\">,<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">title=DAPI_For_Merge<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u5236\u4f5c\u5355\u5f20 DAPI \u56fe<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">selectWindow<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">C1-<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">title<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Flatten<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">rename<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Final_DAPI<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ --- \u5904\u7406 HA-RTCB (C2) ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">selectWindow<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">C2-<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">title<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Red<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u3010Autoscale - \u4fee\u6539\u5904\u3011\u8fd9\u91cc\u4e0d\u518d\u4f7f\u7528 setMinAndMax \u56fa\u5b9a\u503c<\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u800c\u662f\u4f7f\u7528\u81ea\u52a8\u589e\u5f3a\uff0c\u8ba9\u6bcf\u5f20\u56fe\u90fd\u6e05\u6670\u663e\u793a<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Enhance Contrast<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #ECEFF4\">,<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">saturated=0.35<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u590d\u5236\u4e00\u4efd\u7528\u4e8e Merge<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Duplicate...<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #ECEFF4\">,<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">title=HA_For_Merge<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u5236\u4f5c\u5355\u5f20 HA \u56fe<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">selectWindow<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">C2-<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">title<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Flatten<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">rename<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Final_HA<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ --- \u5236\u4f5c Merge \u56fe ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u4f7f\u7528\u521a\u624d\u5df2\u7ecf\u88ab Autoscale \u8fc7\u7684\u56fe\u6765\u5408\u5e76\uff0c\u6240\u4ee5 Merge \u56fe\u4e5f\u4f1a\u5f88\u6f02\u4eae<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Merge Channels...<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #ECEFF4\">,<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">c1=DAPI_For_Merge c2=HA_For_Merge create<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">selectWindow<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Composite<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Flatten<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">rename<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Final_Merge<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ --- \u62fc\u63a5\u4e09\u5f20 RGB \u56fe ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u987a\u5e8f\uff1aDAPI -&gt; HA -&gt; Merge<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Concatenate...<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #ECEFF4\">,<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">  title=&#091;Montage_Stack&#093; image1=Final_DAPI image2=Final_HA image3=Final_Merge image4=&#091;-- None --&#093;<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ --- \u5236\u4f5c\u62fc\u56fe (Montage) ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">selectWindow<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Montage_Stack<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ border=5 \u662f\u56fe\u7247\u4e4b\u95f4\u7684\u767d\u8272\u95f4\u9699<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Make Montage...<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #ECEFF4\">,<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">columns=3 rows=1 scale=1 border=5<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ --- \u52a0\u6bd4\u4f8b\u5c3a ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Scale Bar...<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #ECEFF4\">,<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">width=10 height=8 font=12 color=White background=None location=&#091;Lower Right&#093; bold overlay<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ --- Flatten \u6700\u7ec8\u7ed3\u679c ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">run<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Flatten<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ --- C. \u4fdd\u5b58\u7ed3\u679c ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #D8DEE9\">savePath<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">=<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">outputDir<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">nameWithoutExt<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">_Panel.png<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">saveAs<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">PNG<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #ECEFF4\">,<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">savePath<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ --- D. \u6e05\u7406\u5185\u5b58 ---<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">close<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">*<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">        <\/span><span style=\"color: #616E88\">\/\/ \u6253\u5370\u8fdb\u5ea6<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">        <\/span><span style=\"color: #88C0D0\">print<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">\u5df2\u5904\u7406: <\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #81A1C1\">+<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">filename<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">    <\/span><span style=\"color: #ECEFF4\">}<\/span><\/span>\n<span class=\"line\"><span style=\"color: #ECEFF4\">}<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #88C0D0\">setBatchMode<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #81A1C1\">false<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span>\n<span class=\"line\"><span style=\"color: #88C0D0\">showMessage<\/span><span style=\"color: #D8DEE9FF\">(<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #A3BE8C\">Autoscale \u5904\u7406\u6a21\u5f0f\uff1a\u6240\u6709\u56fe\u7247\u5df2\u5904\u7406\u5b8c\u6bd5\uff01<\/span><span style=\"color: #ECEFF4\">&quot;<\/span><span style=\"color: #D8DEE9FF\">)<\/span><span style=\"color: #81A1C1\">;<\/span><\/span><\/code><\/pre><\/div>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"685\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/08\/20251208095223381-1024x685.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-13600\" style=\"width:799px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/08\/20251208095223381-1024x685.png?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/08\/20251208095223381-300x201.png?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/08\/20251208095223381-768x514.png?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/08\/20251208095223381.png?x-oss-process=style\/default 1187w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd9\u7bc7\u7b14\u8bb0\u8be6\u7ec6\u8bb0\u5f55\u4e86\u4f7f\u7528ImageJ\u5904\u7406\u514d\u75ab\u8367\u5149\u56fe\u7247\u7684\u5168\u6d41\u7a0b\uff0c\u4ece\u57fa\u7840\u7684\u62d6\u62fd\u5bfc\u5165\u3001\u901a\u9053\u62c6\u5206\u3001\u6bd4\u4f8b\u5c3a\u6dfb\u52a0\uff0c\u5230\u8fdb\u9636\u7684\u62fc\u63a5\u3001\u62fc\u56fe(Montage)\u64cd\u4f5c\u3002\u4f5c\u8005\u8fd8\u5206\u4eab\u4e86\u4e00\u4e2a\u5f3a\u5927\u7684\u5168\u81ea\u52a8\u6279\u91cf\u5904\u7406\u5b8f\u811a\u672c\uff0c\u80fd\u4e00\u952e\u5b8c\u6210\u4ece.nd2\u6587\u4ef6\u5bfc\u5165\u5230\u751f\u6210\u6700\u7ec8\u5c55\u793a\u56fe\u7684\u5168\u90e8\u5de5\u4f5c\uff0c\u6781\u5927\u63d0\u5347\u4e86\u6548\u7387\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-11369","post","type-post","status-publish","format-standard","hentry","category-note"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/11369","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11369"}],"version-history":[{"count":7,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/11369\/revisions"}],"predecessor-version":[{"id":13797,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/11369\/revisions\/13797"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11369"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11369"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11369"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}