{"id":13620,"date":"2025-12-13T19:50:04","date_gmt":"2025-12-13T11:50:04","guid":{"rendered":"https:\/\/fanyiming.life\/?p=13620"},"modified":"2025-12-21T01:02:20","modified_gmt":"2025-12-20T17:02:20","slug":"%e8%bd%ac%e5%bd%95%e5%bb%b6%e4%bc%b8%e5%8a%a8%e5%8a%9b%e5%ad%a6%e4%b8%8e%e9%9d%9e%e5%85%b8%e5%9e%8brna%e5%8a%a0%e5%b7%a5%e7%9a%84%e5%8d%8f%e5%90%8c%e8%b0%83%e6%8e%a7%e6%9c%ba%e5%88%b6%ef%bc%9a","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=13620","title":{"rendered":"\u8f6c\u5f55\u5ef6\u4f38\u52a8\u529b\u5b66\u4e0e\u975e\u5178\u578bRNA\u52a0\u5de5\u7684\u534f\u540c\u8c03\u63a7\u673a\u5236\uff1a\u57fa\u4e8eRtcB\u4f9d\u8d56\u578bSplit-GFP\u62a5\u544a\u7cfb\u7edf\u7b5b\u9009\u4e2dMediator\u590d\u5408\u4f53\u6838\u5fc3\u4e9a\u57fa\uff08MED12, 8, 6, 4, 10\uff09\u529f\u80fd\u7684\u6df1\u5ea6\u89e3\u6790\u62a5\u544a"},"content":{"rendered":"\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>By Google Gemini-3-Pro-preview Deep Research<\/p>\n<\/blockquote>\n\n\n\n<h2 class=\"wp-block-heading\">\u6458\u8981<\/h2>\n\n\n\n<p>\u672c\u7814\u7a76\u62a5\u544a\u65e8\u5728\u6df1\u5165\u89e3\u6790\u5728\u4e00\u4e2a\u57fa\u4e8eRtcB\u8fde\u63a5\u9176\u53ca\u6838\u9176\uff08Ribozyme\uff09\u81ea\u526a\u5207\u673a\u5236\u7684Split-GFP RNA\u62a5\u544a\u7cfb\u7edf\u7684\u5927\u89c4\u6a21CRISPR\u7b5b\u9009\u4e2d\uff0cMediator\u590d\u5408\u4f53\u7279\u5b9a\u4e9a\u57fa\u2014\u2014MED12\u3001MED8\u3001MED6\u3001MED4\u53caMED10\u2014\u2014\u88ab\u9274\u5b9a\u4e3a\u5173\u952e\u6fc0\u6d3b\u56e0\u5b50\u7684\u5206\u5b50\u673a\u5236\u3002\u8be5\u62a5\u544a\u7cfb\u7edf\u8bbe\u8ba1\u7cbe\u5999\uff0c\u4f9d\u8d56\u4e8e\u8f6c\u5f55\u540e\u6838\u9176\u7684\u5feb\u901f\u81ea\u526a\u5207\u4ee5\u4ea7\u751f\u7279\u5b9a\u76842&#8242;,3&#8242;-\u73af\u78f7\u9178\uff082&#8242;,3&#8242;&gt;p\uff09\u548c5&#8242;-\u7f9f\u57fa\uff085&#8242;-OH\uff09\u672b\u7aef\uff0c\u968f\u540e\u7531\u5185\u6e90\u6027RtcB\u8fde\u63a5\u9176\u8fdb\u884c\u8fde\u63a5\uff0c\u5e76\u6700\u7ec8\u901a\u8fc7\u526a\u63a5\u4f53\u53bb\u9664\u6b8b\u7559\u5e8f\u5217\u4ee5\u91cd\u7ec4\u5b8c\u6574\u7684GFP\u8bfb\u7801\u6846\u3002Mediator\u4e9a\u57fa\u7684\u7f3a\u5931\u5bfc\u81f4\u62a5\u544a\u4fe1\u53f7\u663e\u8457\u4e0b\u964d\uff0c\u8868\u660e\u8fd9\u4e9b\u56e0\u5b50\u5728\u7ef4\u6301\u8be5\u590d\u6742RNA\u52a0\u5de5\u6d41\u7a0b\u7684\u9ad8\u6548\u6027\u4e2d\u8d77\u7740\u81f3\u5173\u91cd\u8981\u7684\u4e0a\u6e38\u8c03\u63a7\u4f5c\u7528\u3002<\/p>\n\n\n\n<p>\u672c\u62a5\u544a\u7efc\u5408\u5206\u6790\u4e86Mediator\u590d\u5408\u4f53\u7684\u7ed3\u6784\u6a21\u5757\u5b66\u3001RNA\u805a\u5408\u9176II\uff08Pol II\uff09\u7684\u8f6c\u5f55\u5ef6\u4f38\u52a8\u529b\u5b66\u3001\u5171\u8f6c\u5f55RNA\u6298\u53e0\uff08Co-transcriptional RNA folding\uff09\u7684\u751f\u7269\u7269\u7406\u7279\u5f81\u4ee5\u53caRtcB\u8fde\u63a5\u9176\u7684\u8c03\u63a7\u7f51\u7edc\u3002\u5206\u6790\u6307\u51fa\uff0c\u9274\u5b9a\u7684Mediator\u4e9a\u57fa\u5e76\u975e\u4ec5\u4ec5\u4f5c\u4e3a\u901a\u7528\u7684\u8f6c\u5f55\u8d77\u59cb\u56e0\u5b50\uff0c\u800c\u662f\u901a\u8fc7\u7cbe\u7ec6\u8c03\u63a7Pol II\u7684\u6682\u505c\uff08Pausing\uff09\u4e0e\u91ca\u653e\uff08Release\uff09\uff0c\u4e3aTwister\u548cRzb\u6838\u9176\u7684\u6b63\u786e\u6298\u53e0\u4e0e\u6d3b\u6027\u4f4d\u70b9\u5f62\u6210\u63d0\u4f9b\u4e86\u5fc5\u8981\u7684\u201c\u52a8\u529b\u5b66\u7a97\u53e3\u201d\uff08Kinetic Window\uff09\u3002\u7279\u522b\u662f\u6fc0\u9176\u6a21\u5757\u6210\u5458MED12\u7684\u53d1\u73b0\uff0c\u5f3a\u70c8\u63d0\u793a\u4e86\u8f6c\u5f55\u5ef6\u4f38\u901f\u7387\u4e0e\u6838\u9176\u81ea\u526a\u5207\u6548\u7387\u4e4b\u95f4\u7684\u201c\u52a8\u529b\u5b66\u8026\u5408\u201d\uff08Kinetic Coupling\uff09\u662f\u8be5\u7cfb\u7edf\u7684\u9650\u901f\u6b65\u9aa4\u3002\u6b64\u5916\uff0c\u672c\u62a5\u544a\u8fd8\u63a2\u8ba8\u4e86Mediator\u901a\u8fc7\u8c03\u63a7\u672a\u6298\u53e0\u86cb\u767d\u53cd\u5e94\uff08UPR\uff09\u901a\u8def\u95f4\u63a5\u5f71\u54cdRtcB\u6d3b\u6027\u6c34\u5e73\u7684\u53ef\u80fd\u6027\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">\u7b2c\u4e00\u7ae0 \u5f15\u8a00\uff1a\u8f6c\u5f55\u4e0e\u975e\u5178\u578bRNA\u52a0\u5de5\u7684\u4ea4\u6c47\u70b9<\/h2>\n\n\n\n<p>\u5728\u771f\u6838\u751f\u7269\u57fa\u56e0\u8868\u8fbe\u7684\u590d\u6742\u7f51\u7edc\u4e2d\uff0c\u8f6c\u5f55\u673a\u5668\u4e0eRNA\u52a0\u5de5\u673a\u5668\u4e4b\u95f4\u7684\u754c\u9650\u6b63\u53d8\u5f97\u65e5\u76ca\u6a21\u7cca\u3002\u4f20\u7edf\u7684\u89c2\u70b9\u5c06\u8f6c\u5f55\u89c6\u4e3a\u5355\u7eaf\u7684RNA\u5408\u6210\u8fc7\u7a0b\uff0c\u800c\u5c06\u526a\u63a5\u3001\u52a0\u5e3d\u548c\u4fee\u9970\u89c6\u4e3a\u8f6c\u5f55\u540e\u7684\u72ec\u7acb\u4e8b\u4ef6\u3002\u7136\u800c\uff0c\u73b0\u4ee3\u5206\u5b50\u751f\u7269\u5b66\u8bc1\u636e\u8868\u660e\uff0c\u8fd9\u4e9b\u8fc7\u7a0b\u5728\u65f6\u7a7a\u4e0a\u662f\u7d27\u5bc6\u8026\u5408\u7684\u3002\u672c\u62a5\u544a\u6240\u5173\u6ce8\u7684\u7b5b\u9009\u5b9e\u9a8c\uff0c\u5229\u7528\u4e86\u4e00\u4e2a\u9ad8\u5ea6\u5de5\u7a0b\u5316\u7684RNA\u62a5\u544a\u7cfb\u7edf\uff0c\u8be5\u7cfb\u7edf\u5c06\u6838\u9176\u5316\u5b66\u3001\u975e\u5178\u578bRNA\u8fde\u63a5\uff08RtcB\u4f9d\u8d56\uff09\u4ee5\u53ca\u7ecf\u5178\u526a\u63a5\u7ed3\u5408\u5728\u4e00\u8d77\uff0c\u65e0\u610f\u4e2d\u6784\u5efa\u4e86\u4e00\u4e2a\u6781\u5176\u654f\u611f\u7684\u201c\u8f6c\u5f55-\u52a0\u5de5\u8026\u5408\u201d\u63a2\u9488\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">1.1 \u7b5b\u9009\u80cc\u666f\u4e0e\u62a5\u544a\u7cfb\u7edf\u539f\u7406<\/h3>\n\n\n\n<p>\u7528\u6237\u5728IDCP\uff08\u63a8\u6d4b\u4e3aIntrinsically Disordered Protein\u6216\u7279\u5b9a\u9879\u76ee\u4ee3\u53f7\uff09\u76f8\u5173\u7684\u6fc0\u6d3b\u56e0\u5b50\u7b5b\u9009\u4e2d\uff0c\u5229\u7528CRISPR-Cas9\u6572\u9664\u6280\u672f\uff0c\u9274\u5b9a\u51faMediator\u590d\u5408\u4f53\u7684\u4e94\u4e2a\u4e9a\u57fa\uff08MED12, 8, 6, 4, 10\uff09\u4e3a\u8be5\u7cfb\u7edf\u7684\u6b63\u5411\u8c03\u8282\u56e0\u5b50\uff08Activators\uff09\u3002\u8fd9\u610f\u5473\u7740\uff0c\u5f53\u8fd9\u4e9b\u57fa\u56e0\u88ab\u6572\u9664\u65f6\uff0cGFP\u8367\u5149\u4fe1\u53f7\u663e\u8457\u964d\u4f4e\u3002\u4e3a\u4e86\u7406\u89e3\u8fd9\u4e00\u73b0\u8c61\uff0c\u5fc5\u987b\u9996\u5148\u89e3\u6784\u8be5\u62a5\u544a\u7cfb\u7edf\u7684\u751f\u5316\u8fd0\u4f5c\u6d41\u7a0b\uff1a<\/p>\n\n\n\n<ol start=\"1\" class=\"wp-block-list\">\n<li><strong>\u8f6c\u5f55\u5408\u6210\uff08Transcription\uff09\uff1a<\/strong> Pol II\u8f6c\u5f55\u51fa\u4e00\u6761\u5305\u542b [5&#8242;-\u4fa7\u7ffc]&#8211;[N\u7aefGFP]-[\u5185\u542b\u5b50\/\u8fde\u63a5\u5b50]-[C\u7aefGFP]&#8211;[3&#8242;-\u4fa7\u7ffc] \u7684\u524d\u4f53RNA\u3002<\/li>\n\n\n\n<li><strong>\u5171\u8f6c\u5f55\u6838\u9176\u81ea\u526a\u5207\uff08Co-transcriptional Self-cleavage\uff09\uff1a<\/strong> Twister\u548cRzb\uff08\u4e00\u79cdHammerhead\u6838\u9176\u53d8\u4f53\uff09\u5fc5\u987b\u5728\u8f6c\u5f55\u8fc7\u7a0b\u4e2d\u6216\u8f6c\u5f55\u540e\u6781\u77ed\u65f6\u95f4\u5185\u5b8c\u6210\u4e8c\u7ea7\u7ed3\u6784\u6298\u53e0\u5e76\u53d1\u751f\u81ea\u526a\u5207\u3002Twister\u6838\u9176\u901a\u5e38\u5728\u5207\u5272\u4f4d\u70b9\u76845&#8217;\u7aef\u4ea7\u751f2&#8242;,3&#8242;-\u73af\u78f7\u9178\uff0c3&#8217;\u7aef\u4ea7\u751f5&#8242;-\u7f9f\u57fa\uff1bRzb\u540c\u7406\u3002<\/li>\n\n\n\n<li><strong>RtcB\u4ecb\u5bfc\u7684\u8fde\u63a5\uff08RtcB-mediated Ligation\uff09\uff1a<\/strong> \u5185\u6e90\u6027RtcB\uff08HSPC117\uff09\u8bc6\u522b\u4e0a\u6e38\u7247\u6bb5\u76842&#8242;,3&#8242;-\u73af\u78f7\u9178\u672b\u7aef\u548c\u4e0b\u6e38\u7247\u6bb5\u76845&#8242;-\u7f9f\u57fa\u672b\u7aef\uff0c\u50ac\u5316\u5f62\u62103&#8242;,5&#8242;-\u78f7\u9178\u4e8c\u916f\u952e <sup>1<\/sup>\u3002\u8fd9\u4e00\u6b65\u901a\u5e38\u7528\u4e8e\u5c06\u7ebf\u6027\u5206\u5b50\u73af\u5316\uff08Circularization\uff09\u6216\u5c06\u4e24\u4e2a\u72ec\u7acb\u5206\u5b50\u8fde\u63a5\u3002\u5728Split-GFP\u8bed\u5883\u4e0b\uff0c\u8fd9\u6781\u53ef\u80fd\u662f\u901a\u8fc7\u73af\u5316\u5c06\u4e24\u7aef\u8fde\u63a5\uff0c\u6216\u5c06\u4e24\u4e2a\u53cd\u5f0f\u526a\u63a5\u7684\u524d\u4f53\u8fde\u63a5\u3002<\/li>\n\n\n\n<li><strong>\u7ecf\u5178\u526a\u63a5\u4fee\u9970\uff08Splicing Removal of Scars\uff09\uff1a<\/strong> \u8fde\u63a5\u540e\u7684RNA\u5305\u542b\u6838\u9176\u7684\u6b8b\u7559\u5e8f\u5217\u548c\u4eba\u5de5\u8bbe\u8ba1\u7684\u526a\u63a5\u4f4d\u70b9\u3002\u526a\u63a5\u4f53\uff08Spliceosome\uff09\u8bc6\u522b\u4f9b\u4f53\uff08Donor\uff09\u548c\u53d7\u4f53\uff08Acceptor\uff09\u4f4d\u70b9\uff0c\u5207\u9664\u591a\u4f59\u7684\u6838\u9176\u6b8b\u8ff9\u548c\u8fde\u63a5\u5b50\uff0c\u6700\u7ec8\u5f62\u6210\u65e0\u7f1d\u8fde\u63a5\u7684\u5b8c\u6574GFP\u5f00\u653e\u9605\u8bfb\u6846\uff08ORF\uff09\uff0c\u7ffb\u8bd1\u51fa\u529f\u80fd\u6027\u86cb\u767d\u3002<\/li>\n<\/ol>\n\n\n\n<h3 class=\"wp-block-heading\">1.2 \u5173\u952e\u7684\u9650\u901f\u6b65\u9aa4\uff1a\u52a8\u529b\u5b66\u7ade\u4e89<\/h3>\n\n\n\n<p>\u8be5\u7cfb\u7edf\u7684\u6210\u529f\u8fd0\u884c\u6781\u5176\u4f9d\u8d56\u4e8e<strong>\u65f6\u95f4<\/strong>\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6298\u53e0\u4e0e\u5ef6\u4f38\u7684\u7ade\u8d5b\uff1a<\/strong> \u6838\u9176\u5fc5\u987b\u5728\u4e0b\u6e38\u5e8f\u5217\u5e72\u6270\u5176\u6298\u53e0\u4e4b\u524d\uff0c\u6216\u8005\u5728RNA\u88ab\u6838\u9178\u5916\u5207\u9176\u964d\u89e3\u4e4b\u524d\uff0c\u5b8c\u6210\u6298\u53e0\u548c\u5207\u5272\u3002Twister\u6838\u9176\u867d\u7136\u50ac\u5316\u901f\u7387\u6781\u5feb\uff08$k_{obs}$ \u53ef\u8fbe $1000 \\text{ min}^{-1}$\uff09\uff0c\u4f46\u5176\u6298\u53e0\u5bf9\u8f6c\u5f55\u901f\u7387\u9ad8\u5ea6\u654f\u611f <sup>3<\/sup>\u3002<\/li>\n\n\n\n<li><strong>\u8fde\u63a5\u4e0e\u964d\u89e3\u7684\u7ade\u4e89\uff1a<\/strong> \u5207\u5272\u4ea7\u751f\u7684\u672b\u7aef\u5fc5\u987b\u8fc5\u901f\u88abRtcB\u6355\u83b7\u3002\u5982\u679cRtcB\u4e0d\u5728\u9644\u8fd1\uff0c\u6216\u8005\u672b\u7aef\u88ab\u5916\u5207\u9176\uff08\u5982Xrn2\u6216Exosome\uff09\u653b\u51fb\uff0cGFP mRNA\u5c06\u65e0\u6cd5\u5f62\u6210\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u9274\u4e8eMediator\u590d\u5408\u4f53\u5728\u8f6c\u5f55\u8c03\u63a7\u4e2d\u7684\u6838\u5fc3\u5730\u4f4d\uff0c\u5176\u4f5c\u4e3a\u201c\u6fc0\u6d3b\u56e0\u5b50\u201d\u7684\u51fa\u73b0\uff0c\u6697\u793a\u4e86\u8f6c\u5f55\u673a\u5668\u5728\u534f\u8c03\u4e0a\u8ff0\u9650\u901f\u6b65\u9aa4\u4e2d\u626e\u6f14\u4e86\u51b3\u5b9a\u6027\u89d2\u8272\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">\u7b2c\u4e8c\u7ae0 Mediator\u590d\u5408\u4f53\u7684\u7ed3\u6784\u6a21\u5757\u5b66\u4e0e\u529f\u80fd\u5b9a\u4f4d<\/h2>\n\n\n\n<p>Mediator\u662f\u4e00\u4e2a\u5e9e\u5927\u7684\u591a\u4e9a\u57fa\u590d\u5408\u7269\uff08\u4eba\u6e90\u7ea61.4 MDa\uff0c\u5305\u542b\u7ea630\u4e2a\u4e9a\u57fa\uff09\uff0c\u5728\u7ed3\u6784\u4e0a\u88ab\u5212\u5206\u4e3a\u5934\u90e8\uff08Head\uff09\u3001\u4e2d\u90e8\uff08Middle\uff09\u3001\u5c3e\u90e8\uff08Tail\uff09\u4ee5\u53ca\u53ef\u89e3\u79bb\u7684\u6fc0\u9176\u6a21\u5757\uff08Kinase Module\/CKM\uff09<sup>5<\/sup>\u3002\u7b5b\u9009\u4e2d\u547d\u4e2d\u7684\u4e94\u4e2a\u4e9a\u57fa\u5e76\u975e\u968f\u673a\u5206\u5e03\uff0c\u800c\u662f\u52fe\u52d2\u51fa\u4e86\u4e00\u6761\u8d2f\u7a7fMediator\u6838\u5fc3\u67b6\u6784\u7684\u201c\u810a\u6881\u201d\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">2.1 \u5934\u90e8\u6a21\u5757\uff08Head Module\uff09\uff1aMED6\u4e0eMED8 \u2014\u2014 Pol II\u7684\u76f4\u63a5\u62db\u52df\u8005<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>MED8\u7684\u7ed3\u6784\u4e0e\u529f\u80fd\uff1aMED8\u662f\u5934\u90e8\u6a21\u5757\u7684\u6838\u5fc3\u9aa8\u67b6\u6210\u5206\uff0c\u4e0eMED18\u548cMED20\u5f62\u6210\u7d27\u5bc6\u590d\u5408\u7269\u3002\u7ed3\u6784\u751f\u7269\u5b66\u7814\u7a76\uff08\u51b7\u51bb\u7535\u955cCryo-EM\uff09\u663e\u793a\uff0cMED8\u4f4d\u4e8e\u5934\u90e8\u6a21\u5757\u7684\u201c\u81c2\u201d\uff08Arm\uff09\u7ed3\u6784\u57df\uff0c\u76f4\u63a5\u53c2\u4e0e\u540cRNA\u805a\u5408\u9176II\u7684Rpb4\/7\u4e9a\u57fa\u67c4\u90e8\uff08Stalk\uff09\u4ee5\u53caCTD\uff08C\u672b\u7aef\u7ed3\u6784\u57df\uff09\u7684\u7269\u7406\u63a5\u89e6 5\u3002\n<ul class=\"wp-block-list\">\n<li><strong>\u5728\u7b5b\u9009\u4e2d\u7684\u610f\u4e49\uff1a<\/strong> MED8\u7684\u7f3a\u5931\u4f1a\u76f4\u63a5\u7834\u574fMediator\u5934\u90e8\u4e0ePol II\u7684\u7ed3\u5408\u754c\u9762\u3002\u5728\u9175\u6bcd\u548c\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\uff0cMED8\u7684\u7f3a\u5931\u901a\u5e38\u5bfc\u81f4\u5168\u57fa\u56e0\u7ec4\u8303\u56f4\u5185Pol II\u62db\u52df\u7684\u707e\u96be\u6027\u4e0b\u964d <sup>8<\/sup>\u3002\u56e0\u6b64\uff0cMED8\u4f5c\u4e3a\u6fc0\u6d3b\u56e0\u5b50\u7684\u6700\u76f4\u63a5\u89e3\u91ca\u662f\uff1a<strong>\u6ca1\u6709MED8\uff0c\u5c31\u6ca1\u6709PIC\uff08\u9884\u8d77\u59cb\u590d\u5408\u7269\uff09\u7684\u7ec4\u88c5\uff0c\u4e5f\u5c31\u6ca1\u6709\u62a5\u544a\u57fa\u56e0\u7684\u8f6c\u5f55\u8d77\u59cb\u3002<\/strong><\/li>\n<\/ul>\n<\/li>\n\n\n\n<li>MED6\u7684\u7ed3\u6784\u4e0e\u529f\u80fd\uff1aMED6\u662f\u8fde\u63a5\u5934\u90e8\u4e0e\u4e2d\u90e8\u6a21\u5757\u7684\u5173\u952e\u6865\u6881\u3002\u5b83\u901a\u8fc7\u5176C\u7aef\u7ed3\u6784\u57df\u4e0e\u4e2d\u90e8\u6a21\u5757\u7684MED4\u53caMED31\u76f8\u4e92\u4f5c\u7528\uff0c\u540c\u65f6\u5176N\u7aef\u53c2\u4e0e\u6784\u6210\u5934\u90e8\u6a21\u5757\u7684\u4e3b\u4f53 5\u3002MED6\u4e0d\u4ec5\u5bf9\u4e8e\u7ef4\u6301Mediator\u7684\u6574\u4f53\u5b8c\u6574\u6027\u81f3\u5173\u91cd\u8981\uff0c\u8fd8\u88ab\u8bc1\u660e\u5728\u6fc0\u6d3b\u56e0\u5b50\u4f9d\u8d56\u7684\u8f6c\u5f55\u4e2d\u5fc5\u4e0d\u53ef\u5c11\u3002\n<ul class=\"wp-block-list\">\n<li><strong>\u5728\u7b5b\u9009\u4e2d\u7684\u610f\u4e49\uff1a<\/strong> MED6\u7684\u7f3a\u5931\u4f1a\u5bfc\u81f4\u5934\u90e8\u6a21\u5757\u4e0e\u4e2d\u90e8\u6a21\u5757\u89e3\u79bb\uff0c\u4f7f\u5f97Mediator\u65e0\u6cd5\u5c06\u5c3e\u90e8\u6a21\u5757\u63a5\u6536\u5230\u7684\u4fe1\u53f7\u4f20\u9012\u7ed9Pol II\uff0c\u540c\u6837\u5bfc\u81f4\u8f6c\u5f55\u8d77\u59cb\u7684\u4e25\u91cd\u7f3a\u9677\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">2.2 \u4e2d\u90e8\u6a21\u5757\uff08Middle Module\uff09\uff1aMED4\u4e0eMED10 \u2014\u2014 \u4fe1\u53f7\u8f6c\u5bfc\u7684\u7ed3\u6784\u67a2\u7ebd<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>MED4\u7684\u7ed3\u6784\u4e0e\u529f\u80fd\uff1aMED4\u4f4d\u4e8e\u4e2d\u90e8\u6a21\u5757\u7684\u201c\u7403\u94ae\u201d\uff08Knob\uff09\u533a\u57df\uff0c\u662f\u4e00\u4e2a\u6781\u5176\u5173\u952e\u7684\u7ed3\u6784\u4e9a\u57fa\u3002\u5b83\u50cf\u4e00\u4e2a\u94f0\u94fe\uff0c\u4e00\u65b9\u9762\u901a\u8fc7MED6\u8fde\u63a5\u5934\u90e8\u6a21\u5757\uff0c\u53e6\u4e00\u65b9\u9762\u901a\u8fc7MED14\uff08Mediator\u7684\u810a\u67f1\uff09\u8fde\u63a5\u5c3e\u90e8\u6a21\u5757 5\u3002MED4\u8fd8\u88ab\u62a5\u9053\u53c2\u4e0e\u67d3\u8272\u8d28\u73af\u7684\u5f62\u6210\u53ca\u8f6c\u5f55\u5ef6\u4f38\u56e0\u5b50\u7684\u62db\u52df\u3002\n<ul class=\"wp-block-list\">\n<li><strong>\u5728\u7b5b\u9009\u4e2d\u7684\u610f\u4e49\uff1a<\/strong> MED4\u7684\u6572\u9664\u4f1a\u74e6\u89e3Mediator\u7684\u6838\u5fc3\u67b6\u6784\uff0c\u5bfc\u81f4\u5934\u90e8\u548c\u5c3e\u90e8\u529f\u80fd\u7684\u8131\u8282\u3002\u6b64\u5916\uff0cMED4\u88ab\u8ba4\u4e3a\u5728\u4fc3\u8fdbPIC\u7ec4\u88c5\u540e\u7684\u6784\u8c61\u53d8\u5316\u4e2d\u8d77\u4f5c\u7528\uff0c\u8fd9\u5bf9Pol II\u4ece\u542f\u52a8\u5b50\u9003\u9038\uff08Promoter Escape\uff09\u81f3\u5173\u91cd\u8981\u3002<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li>MED10 (Nut2) \u7684\u7ed3\u6784\u4e0e\u529f\u80fd\uff1aMED10\u662f\u4e2d\u90e8\u6a21\u5757\u201c\u94a9\u201d\uff08Hook\uff09\u7ed3\u6784\u57df\u7684\u7ec4\u6210\u90e8\u5206\u3002\u5b83\u4e0eMED14\u548cMED19\u76f8\u4e92\u4f5c\u7528\uff0c\u5bf9\u4e8e\u7ef4\u6301\u4e2d\u90e8\u6a21\u5757\u7684\u7a33\u5b9a\u6027\u81f3\u5173\u91cd\u8981 5\u3002\n<ul class=\"wp-block-list\">\n<li><strong>\u5728\u7b5b\u9009\u4e2d\u7684\u610f\u4e49\uff1a<\/strong> MED10\u7684\u7f3a\u5931\u540c\u6837\u4f1a\u5bfc\u81f4Mediator\u6838\u5fc3\u529f\u80fd\u7684\u4e27\u5931\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0cMED10\u5728\u67d0\u4e9b\u8bed\u5883\u4e0b\u88ab\u53d1\u73b0\u4e0e\u8f6c\u5f55\u5ef6\u4f38\u56e0\u5b50\u6709\u7279\u5b9a\u7684\u9057\u4f20\u76f8\u4e92\u4f5c\u7528\uff0c\u6697\u793a\u5176\u529f\u80fd\u53ef\u80fd\u5ef6\u4f38\u81f3\u8d77\u59cb\u4e4b\u540e <sup>11<\/sup>\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">2.3 \u6fc0\u9176\u6a21\u5757\uff08Kinase Module\uff09\uff1aMED12 \u2014\u2014 \u8f6c\u5f55\u5ef6\u4f38\u4e0e\u5e94\u6fc0\u53cd\u5e94\u7684\u8c03\u63a7\u8005<\/h3>\n\n\n\n<p>MED12\u7684\u51fa\u73b0\u662f\u672c\u6b21\u7b5b\u9009\u4e2d\u6700\u5177\u673a\u5236\u542f\u793a\u6027\u7684\u53d1\u73b0\u3002\u4e0eMED4\/6\/8\/10\u4f5c\u4e3a\u201c\u6838\u5fc3\u201d\u7ed3\u6784\u6210\u5206\u4e0d\u540c\uff0cMED12\u5c5e\u4e8e\u53ef\u89e3\u79bb\u7684<strong>CDK8\u6fc0\u9176\u6a21\u5757\uff08CKM\uff09<\/strong>\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>MED12\u7684\u7ed3\u6784\u4e0e\u529f\u80fd\uff1aMED12\u662fCKM\u4e2d\u6700\u5927\u7684\u4e9a\u57fa\uff0c\u5b83\u5145\u5f53\u652f\u67b6\uff0c\u5c06CDK8\uff08\u6fc0\u9176\uff09\u3001Cyclin C\uff08CycC\uff09\u548cMED13\u951a\u5b9a\u5728Core Mediator\u4e0a 7\u3002\n<ul class=\"wp-block-list\">\n<li><strong>\u53cc\u91cd\u529f\u80fd\uff1a<\/strong> \u4f20\u7edf\u4e0a\uff0cCKM\u56e0\u5176\u7a7a\u95f4\u4f4d\u963b\u963b\u788dPol II\u7ed3\u5408\u800c\u88ab\u89c6\u4e3a\u8f6c\u5f55<strong>\u6291\u5236\u5b50<\/strong>\u3002\u7136\u800c\uff0c\u5927\u91cf\u73b0\u4ee3\u7814\u7a76\u8868\u660e\uff0cMED12\u548cCDK8\u5728\u7279\u5b9a\u7684\u57fa\u56e0\u7f51\u7edc\uff08\u5982HIF1A\u3001Wnt\u3001\u8840\u6e05\u53cd\u5e94\u57fa\u56e0\uff09\u4e2d\u662f\u5173\u952e\u7684<strong>\u5171\u6fc0\u6d3b\u56e0\u5b50<\/strong> <sup>13<\/sup>\u3002<\/li>\n\n\n\n<li><strong>\u5ef6\u4f38\u8c03\u63a7\uff1a<\/strong> MED12\/CDK8\u590d\u5408\u7269\u88ab\u8bc1\u5b9e\u80fd\u62db\u52df<strong>\u8d85\u7ea7\u5ef6\u4f38\u590d\u5408\u7269\uff08Super Elongation Complex, SEC\uff09\u548cP-TEFb<\/strong>\uff08\u542bCDK9\uff09\u3002CDK9\u78f7\u9178\u5316Pol II CTD\u7684Ser2\u4f4d\u70b9\u4ee5\u53ca\u8d1f\u5411\u5ef6\u4f38\u56e0\u5b50\uff08NELF\/DSIF\uff09\uff0c\u4ece\u800c\u91ca\u653e\u6682\u505c\u7684Pol II\uff0c\u542f\u52a8\u751f\u4ea7\u6027\u5ef6\u4f38\uff08Productive Elongation\uff09 <sup>13<\/sup>\u3002<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>\u5728\u7b5b\u9009\u4e2d\u7684\u610f\u4e49\uff1a<\/strong> MED12\u4f5c\u4e3a\u6fc0\u6d3b\u56e0\u5b50\uff08KO\u5bfc\u81f4\u4fe1\u53f7\u4e0b\u964d\uff09\uff0c\u5f3a\u70c8\u6697\u793a\u8be5\u62a5\u544a\u7cfb\u7edf\u4e0d\u4ec5\u9700\u8981\u8f6c\u5f55\u8d77\u59cb\uff08\u7531Core Mediator\u8d1f\u8d23\uff09\uff0c\u66f4\u6781\u5ea6\u4f9d\u8d56\u4e8e<strong>\u9ad8\u6548\u7684\u8f6c\u5f55\u5ef6\u4f38<\/strong>\u3002\u5982\u679cPol II\u5728\u8f6c\u5f55\u8fc7\u7a0b\u4e2d\u505c\u6ede\u6216\u8131\u843d\uff0c\u5c31\u65e0\u6cd5\u5408\u6210\u5305\u542b\u5b8c\u6574\u6838\u9176\u5e8f\u5217\u7684\u5168\u957fRNA\uff0c\u540e\u7eed\u7684RtcB\u8fde\u63a5\u4e5f\u5c31\u65e0\u4ece\u8c08\u8d77\u3002<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">\u7b2c\u4e09\u7ae0 \u6838\u5fc3\u673a\u5236\u6a21\u578b\u4e00\uff1a\u52a8\u529b\u5b66\u8026\u5408\u4e0e\u5171\u8f6c\u5f55\u6838\u9176\u6298\u53e0<\/h2>\n\n\n\n<p>\u672c\u62a5\u544a\u63d0\u51fa\u7684\u9996\u8981\u673a\u5236\u6a21\u578b\u662f\u201c\u52a8\u529b\u5b66\u8026\u5408\u201d\uff08Kinetic Coupling\uff09\u3002\u8be5\u6a21\u578b\u8ba4\u4e3a\uff0cMediator\u590d\u5408\u4f53\uff08\u7279\u522b\u662fMED12\uff09\u901a\u8fc7\u8c03\u63a7Pol II\u7684\u5ef6\u4f38\u901f\u7387\uff0c\u4e3aTwister\u548cRzb\u6838\u9176\u7684\u6b63\u786e\u6298\u53e0\u521b\u9020\u4e86\u5fc5\u8981\u7684\u751f\u7269\u7269\u7406\u6761\u4ef6\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">3.1 \u5171\u8f6c\u5f55\u6298\u53e0\u7684\u201c\u65f6\u95f4\u7a97\u201d\u7406\u8bba<\/h3>\n\n\n\n<p>RNA\u7684\u6298\u53e0\u662f\u5728\u8f6c\u5f55\u8fc7\u7a0b\u4e2d\u5b9e\u65f6\u53d1\u751f\u7684\uff08Co-transcriptional folding\uff09\u3002Pol II\u4ee5\u6bcf\u79d220-70\u4e2a\u6838\u82f7\u9178\u7684\u901f\u5ea6\u5408\u6210RNA\u3002\u8fd9\u610f\u5473\u7740RNA\u76845&#8217;\u7aef\u4f1a\u57283&#8217;\u7aef\u5408\u6210\u4e4b\u524d\u5148\u6298\u53e0\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6838\u9176\u7684\u6298\u53e0\u56f0\u5883\uff1a<\/strong> Twister\u548cHammerhead\u7b49\u5c0f\u6838\u9176\u5f62\u6210\u6d3b\u6027\u6784\u8c61\u4f9d\u8d56\u4e8e\u7279\u5b9a\u7684\u4e8c\u7ea7\u548c\u4e09\u7ea7\u76f8\u4e92\u4f5c\u7528\u3002\u5982\u679c\u8f6c\u5f55\u901f\u5ea6\u8fc7\u5feb\uff0c\u4e0b\u6e38\u65b0\u5408\u6210\u7684RNA\u5e8f\u5217\u53ef\u80fd\u4f1a\u4e0e\u4e0a\u6e38\u5c1a\u672a\u6298\u53e0\u597d\u7684\u6838\u9176\u5e8f\u5217\u53d1\u751f\u975e\u7279\u5f02\u6027\u78b1\u57fa\u914d\u5bf9\uff0c\u5f62\u6210\u70ed\u529b\u5b66\u4e0a\u7a33\u5b9a\u4f46\u529f\u80fd\u4e0a\u5931\u6d3b\u7684\u201c\u6b7b\u80e1\u540c\u201d\u7ed3\u6784\uff08Misfolded intermediates \u6216 Ribozymogens\uff09<sup>16<\/sup>\u3002<\/li>\n\n\n\n<li><strong>\u6682\u505c\uff08Pausing\uff09\u7684\u91cd\u8981\u6027\uff1a<\/strong> \u4e3a\u4e86\u907f\u514d\u8fd9\u79cd\u5e72\u6270\uff0cPol II\u901a\u5e38\u9700\u8981\u5728\u6838\u9176\u5e8f\u5217\u8f6c\u5f55\u5b8c\u6210\u540e\u7684\u7279\u5b9a\u4f4d\u70b9\u8fdb\u884c\u201c\u8f6c\u5f55\u6682\u505c\u201d\u3002\u8fd9\u79cd\u6682\u505c\u4e3a\u65b0\u751fRNA\u94fe\u63d0\u4f9b\u4e86\u5b9d\u8d35\u7684\u6beb\u79d2\u81f3\u79d2\u7ea7\u7684\u65f6\u95f4\u7a97\u53e3\uff0c\u4f7f\u5176\u80fd\u591f\u5728\u4e0d\u53d7\u4e0b\u6e38\u5e8f\u5217\u5e72\u6270\u7684\u60c5\u51b5\u4e0b\uff0c\u63a2\u7d22\u5e76\u9501\u5b9a\u539f\u672c\u7684\u50ac\u5316\u6784\u8c61 <sup>18<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">3.2 MED12\u4f5c\u4e3a\u5ef6\u4f38\u901f\u7387\u7684\u201c\u8282\u62cd\u5668\u201d<\/h3>\n\n\n\n<p>\u7b5b\u9009\u7ed3\u679c\u663e\u793aMED12\u662f\u6fc0\u6d3b\u56e0\u5b50\uff0c\u8fd9\u610f\u5473\u7740\u5728MED12\u7f3a\u5931\u7684\u7ec6\u80de\u4e2d\uff0c\u62a5\u544a\u57fa\u56e0\u7684\u8868\u8fbe\u6216\u52a0\u5de5\u6548\u7387\u5927\u5e45\u4e0b\u964d\u3002\u7ed3\u5408MED12\u5728\u8c03\u63a7Pol II\u6682\u505c\u91ca\u653e\u4e2d\u7684\u5df2\u77e5\u529f\u80fd <sup>13<\/sup>\uff0c\u6211\u4eec\u53ef\u4ee5\u63a8\u5bfc\u51fa\u4ee5\u4e0b\u75c5\u7406\u673a\u5236\uff1a<\/p>\n\n\n\n<ol start=\"1\" class=\"wp-block-list\">\n<li><strong>\u91ce\u751f\u578b\u7ec6\u80de\uff08WT\uff09\uff1a<\/strong> MED12\/CDK8\u62db\u52dfSEC\/P-TEFb\u3002Pol II\u5728\u542f\u52a8\u5b50\u8fd1\u7aef\u6682\u505c\uff08Promoter-Proximal Pausing\uff09\u540e\u88ab\u6709\u5e8f\u91ca\u653e\u8fdb\u5165\u5ef6\u4f38\u9636\u6bb5\u3002\u5728\u5ef6\u4f38\u8fc7\u7a0b\u4e2d\uff0cMediator\u53ef\u80fd\u534f\u52a9\u7ef4\u6301Pol II\u7684\u6301\u7eed\u5408\u6210\u80fd\u529b\uff08Processivity\uff09\uff0c\u9632\u6b62\u5176\u5728\u9047\u5230\u6838\u9176\u5f62\u6210\u7684\u590d\u6742\u4e8c\u7ea7\u7ed3\u6784\u65f6\u53d1\u751f\u8fc7\u65e9\u7ec8\u6b62\uff08Premature Termination\uff09\u3002\u540c\u65f6\uff0c\u9002\u5f53\u7684\u5ef6\u4f38\u901f\u7387\u4fdd\u8bc1\u4e86\u6838\u9176\u6709\u65f6\u95f4\u6b63\u786e\u6298\u53e0\u3002<\/li>\n\n\n\n<li><strong>MED12\u6572\u9664\u7ec6\u80de\uff08KO\uff09\uff1a<\/strong>\n<ul class=\"wp-block-list\">\n<li><strong>\u60c5\u666fA\uff08\u5ef6\u4f38\u969c\u788d\uff09\uff1a<\/strong> \u7f3a\u4e4fMED12\u5bfc\u81f4\u7684P-TEFb\u62db\u52df\u4e0d\u8db3\uff0c\u4f7f\u5f97Pol II\u65e0\u6cd5\u6709\u6548\u514b\u670d\u6838\u9176\u5e8f\u5217\u672c\u8eab\u6784\u6210\u7684\u8f6c\u5f55\u969c\u788d\uff08RNA\u53d1\u5361\u7ed3\u6784\u5f80\u5f80\u662f\u5929\u7136\u7684\u8f6c\u5f55\u6682\u505c\u6216\u7ec8\u6b62\u4fe1\u53f7\uff09\u3002Pol II\u53ef\u80fd\u5728\u5408\u6210\u5b8c\u5b8c\u6574\u7684GFP\u5e8f\u5217\u4e4b\u524d\u5c31\u505c\u6ede\u6216\u8131\u843d\u3002\u7ed3\u679c\u662f\uff1a\u5168\u957f\u8f6c\u5f55\u672c\u51cf\u5c11\uff0c\u5e95\u7269\u4e0d\u8db3\u3002<\/li>\n\n\n\n<li><strong>\u60c5\u666fB\uff08\u6682\u505c\u91ca\u653e\u5931\u8c03\uff09\uff1a<\/strong> \u5728\u67d0\u4e9b\u60c5\u51b5\u4e0b\uff0cCDK8\/MED12\u7684\u7f3a\u5931\u53ef\u80fd\u5bfc\u81f4Pol II\u5728\u57fa\u56e0\u4f53\u5185\u7684\u6682\u505c\u5206\u5e03\u5f02\u5e38\u3002\u5982\u679cPol II\u5728\u5173\u952e\u7684\u6298\u53e0\u4f4d\u70b9\u6ca1\u6709\u9002\u5f53\u6682\u505c\uff0c\u6216\u8005\u56e0\u7f3a\u4e4f\u6301\u7eed\u5408\u6210\u80fd\u529b\u800c\u5bfc\u81f4\u5ef6\u4f38\u901f\u7387\u6781\u4e0d\u7a33\u5b9a\uff0cTwister\u548cRzb\u6838\u9176\u53ef\u80fd\u65e0\u6cd5\u5f62\u6210\u6d3b\u6027\u6784\u8c61\u3002\u672a\u526a\u5207\u7684\u524d\u4f53RNA\u65e0\u6cd5\u4ea7\u751f2&#8242;,3&#8242;-\u73af\u78f7\u9178\u672b\u7aef\uff0c\u56e0\u6b64\u65e0\u6cd5\u88abRtcB\u8bc6\u522b\u8fde\u63a5\u3002<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n\n\n\n<h3 class=\"wp-block-heading\">3.3 \u6570\u636e\u652f\u6491\uff1aTwister\u6838\u9176\u5bf9\u8f6c\u5f55\u73af\u5883\u7684\u654f\u611f\u6027<\/h3>\n\n\n\n<p>\u7814\u7a76\u8868\u660e\uff0cTwister\u6838\u9176\u7684\u81ea\u526a\u5207\u6d3b\u6027\u5e76\u975e\u603b\u662f100%\u9ad8\u6548\uff0c\u800c\u662f\u53d7\u5230\u4fa7\u7ffc\u5e8f\u5217\u7684\u663e\u8457\u5f71\u54cd <sup>16<\/sup>\u3002\u5728\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\uff0cTwister\u7684\u5171\u8f6c\u5f55\u526a\u5207\u6548\u7387\u76f4\u63a5\u51b3\u5b9a\u4e86\u73af\u72b6RNA\u6216\u8fde\u63a5\u4ea7\u7269\u7684\u4ea7\u7387 <sup>20<\/sup>\u3002\u5982\u679cMediator\u7684\u7f3a\u5931\u5e72\u6270\u4e86Pol II\u901a\u8fc7\u8fd9\u4e9b\u9ad8\u7ed3\u6784\u5316\u533a\u57df\u7684\u80fd\u529b\uff0c\u6216\u8005\u6539\u53d8\u4e86\u5c40\u90e8\u67d3\u8272\u8d28\u73af\u5883\u4f7f\u5f97\u5ef6\u4f38\u53d8\u5f97\u201c\u98a0\u7c38\u201d\uff08jerky\uff09\uff0c\u6838\u9176\u7684\u6298\u53e0\u8def\u5f84\u5c31\u4f1a\u53d1\u751f\u504f\u79bb\uff0c\u5bfc\u81f4\u7cfb\u7edf\u5931\u6548\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">\u7b2c\u56db\u7ae0 \u6838\u5fc3\u673a\u5236\u6a21\u578b\u4e8c\uff1a\u8f6c\u5f55\u4e0e\u526a\u63a5\u7684\u76f4\u63a5\u62db\u52df\u4e0e\u534f\u540c<\/h2>\n\n\n\n<p>\u62a5\u544a\u7cfb\u7edf\u660e\u786e\u63d0\u5230\uff1a\u201c\u901a\u8fc7\u526a\u63a5\u53bb\u9664\u6389\u6838\u9176\u65ad\u88c2\u540e\u6b8b\u7559\u7684\u672b\u7aef\uff0c\u5f62\u6210\u4e00\u4e2a\u65e0\u7f1d\u8fde\u63a5\u7684\u5b8c\u6574\u7684 GFP RNA\u201d\u3002\u8fd9\u5f15\u5165\u4e86\u7b2c\u4e8c\u5c42\u590d\u6742\u7684\u8c03\u63a7\u673a\u5236\u2014\u2014<strong>\u526a\u63a5<\/strong>\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">4.1 Mediator\u4e0e\u526a\u63a5\u673a\u5668\u7684\u7269\u7406\u4e92\u4f5c<\/h3>\n\n\n\n<p>\u5c3d\u7ba1\u526a\u63a5\u4e3b\u8981\u7531\u526a\u63a5\u4f53\u6267\u884c\uff0c\u4f46\u8d8a\u6765\u8d8a\u591a\u7684\u8bc1\u636e\u8868\u660e\uff0cMediator\u5728\u8fde\u63a5\u8f6c\u5f55\u4e0e\u526a\u63a5\u4e2d\u8d77\u7740\u6865\u6881\u4f5c\u7528\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u62db\u52df\u529f\u80fd\uff1a<\/strong> Mediator\u4e9a\u57fa\uff08\u5982MED23\uff09\u5df2\u88ab\u8bc1\u660e\u80fd\u4e0e\u526a\u63a5\u56e0\u5b50\uff08\u5982hnRNP L\uff09\u548c\u5bcc\u542b\u4e1d\u6c28\u9178\/\u7cbe\u6c28\u9178\u7684\u86cb\u767d\uff08SR proteins\uff09\u76f8\u4e92\u4f5c\u7528 <sup>21<\/sup>\u3002<\/li>\n\n\n\n<li><strong>MED12\u7684\u89d2\u8272\uff1a<\/strong> MED12\u4f5c\u4e3aKinase\u6a21\u5757\u7684\u951a\u5b9a\u70b9\uff0c\u5176\u5de8\u5927\u7684\u8868\u9762\u79ef\u4e3a\u591a\u79cd\u6838\u5185\u56e0\u5b50\u63d0\u4f9b\u4e86\u7ed3\u5408\u5e73\u53f0\u3002\u5728\u62a5\u544a\u7cfb\u7edf\u4e2d\uff0cRtcB\u8fde\u63a5\u540e\u7684\u4ea7\u7269\u662f\u4e00\u4e2a\u975e\u5178\u578b\u7684\u526a\u63a5\u5e95\u7269\uff08\u53ef\u80fd\u662f\u73af\u72b6\u6216\u901a\u8fc7trans-ligation\u5f62\u6210\u7684\u7ebf\u6027\u5206\u5b50\uff09\u3002\u8fd9\u79cd\u7279\u6b8a\u7684\u5e95\u7269\u53ef\u80fd\u9700\u8981\u9ad8\u6548\u7684\u526a\u63a5\u4f53\u62db\u52df\u624d\u80fd\u5b8c\u6210\u6700\u540e\u7684\u201c\u53bb\u75a4\u75d5\u201d\u6b65\u9aa4\u3002<\/li>\n\n\n\n<li><strong>\u5047\u8bbe\uff1a<\/strong> MED12\u53ef\u80fd\u534f\u52a9\u62db\u52df\u4e86\u7279\u5b9a\u7684\u526a\u63a5\u56e0\u5b50\u6216RtcB\u590d\u5408\u4f53\u672c\u8eab\uff08\u5305\u542bDDX1\u7b49\u89e3\u65cb\u9176\uff09\u5230\u8f6c\u5f55\u4f4d\u70b9\u3002DDX1\u5df2\u77e5\u80fd\u89e3\u5f00RNA\u4e8c\u7ea7\u7ed3\u6784\uff0c\u5bf9\u4e8eRtcB\u7684\u8fde\u63a5\u6d3b\u6027\u81f3\u5173\u91cd\u8981 <sup>2<\/sup>\u3002\u5982\u679cMED12\u7f3a\u5931\uff0cDDX1\u7b49\u8f85\u52a9\u56e0\u5b50\u65e0\u6cd5\u6709\u6548\u5bcc\u96c6\u5728\u65b0\u751f\u8f6c\u5f55\u672c\u5468\u56f4\uff0c\u5bfc\u81f4\u8fde\u63a5\u6216\u968f\u540e\u7684\u526a\u63a5\u6548\u7387\u4f4e\u4e0b\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">4.2 \u5ef6\u4f38\u901f\u7387\u5bf9\u526a\u63a5\u4f4d\u70b9\u9009\u62e9\u7684\u5f71\u54cd<\/h3>\n\n\n\n<p>\u52a8\u529b\u5b66\u8026\u5408\u6a21\u578b\u540c\u6837\u9002\u7528\u4e8e\u526a\u63a5\u3002\u6839\u636e\u201c\u52a8\u529b\u5b66\u6a21\u578b\u201d\uff08Kinetic Model\uff09\uff0cPol II\u7684\u5ef6\u4f38\u901f\u5ea6\u51b3\u5b9a\u4e86\u5f31\u526a\u63a5\u4f4d\u70b9\u662f\u5426\u80fd\u88ab\u8bc6\u522b\uff08\u5916\u663e\u5b50\u8df3\u8dc3 vs \u5305\u542b\uff09<sup>22<\/sup>\u3002\u672c\u62a5\u544a\u7cfb\u7edf\u4e2d\u7684\u4eba\u5de5\u526a\u63a5\u4f4d\u70b9\u53ef\u80fd\u5e76\u975e\u6700\u4f18\u5316\u7684\u5f3a\u4f4d\u70b9\uff0c\u56e0\u6b64\u5bf9Pol II\u7684\u901f\u5ea6\u6781\u5176\u654f\u611f\u3002MED12\u7f3a\u5931\u5bfc\u81f4\u7684\u5ef6\u4f38\u901f\u7387\u6539\u53d8\u53ef\u80fd\u7834\u574f\u4e86\u5bf9\u5916\u663e\u5b50\/\u526a\u63a5\u4f4d\u70b9\u7684\u6b63\u786e\u8bc6\u522b\uff0c\u5bfc\u81f4\u526a\u63a5\u5931\u8d25\u6216\u9519\u8bef\u526a\u63a5\uff0c\u65e0\u6cd5\u4ea7\u751f\u529f\u80fd\u6027GFP\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">\u7b2c\u4e94\u7ae0 \u6838\u5fc3\u673a\u5236\u6a21\u578b\u4e09\uff1aRtcB\u7684\u8f6c\u5f55\u8c03\u63a7\u4e0e\u672a\u6298\u53e0\u86cb\u767d\u53cd\u5e94\uff08UPR\uff09<\/h2>\n\n\n\n<p>\u9664\u4e86\u76f4\u63a5\u53c2\u4e0e\u62a5\u544a\u57fa\u56e0\u7684\u8f6c\u5f55\u548c\u52a0\u5de5\uff0cMediator\u8fd8\u53ef\u80fd\u901a\u8fc7\u8c03\u63a7<strong>\u5185\u6e90\u6027RtcB\u7684\u8868\u8fbe\u91cf<\/strong>\u6216\u6d3b\u6027\u72b6\u6001\u6765\u5f71\u54cd\u7cfb\u7edf\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">5.1 RtcB\u4e0eUPR\u901a\u8def<\/h3>\n\n\n\n<p>RtcB\u662f\u771f\u6838\u751f\u7269\u4e2d\u8d1f\u8d23<strong>XBP1 mRNA<\/strong>\u975e\u5e38\u89c4\u526a\u63a5\u7684\u8fde\u63a5\u9176\uff0c\u8fd9\u662f\u672a\u6298\u53e0\u86cb\u767d\u53cd\u5e94\uff08UPR\uff09\u4e2dIRE1\u901a\u8def\u7684\u5173\u952e\u6b65\u9aa4 <sup>1<\/sup>\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>MED12\u4e0eUPR\uff1a<\/strong> MED12\u88ab\u8bc1\u5b9e\u662fUPR\u4e0b\u6e38\u57fa\u56e0\u8868\u8fbe\u7684\u5173\u952e\u8c03\u63a7\u8005\u3002\u5b83\u65e2\u53ef\u4ee5\u4f5c\u4e3a\u6291\u5236\u5b50\u9632\u6b62\u57fa\u5e95\u6c34\u5e73\u7684UPR\u6fc0\u6d3b\uff0c\u4e5f\u662f\u5e94\u6fc0\u72b6\u6001\u4e0b\u8bf1\u5bfcUPR\u9776\u57fa\u56e0\u6240\u5fc5\u9700\u7684 <sup>25<\/sup>\u3002<\/li>\n\n\n\n<li><strong>\u5173\u8054\u6027\uff1a<\/strong> \u62a5\u544a\u7cfb\u7edf\u4e2d\u4ea7\u751f\u7684\u5927\u91cf\u5e26\u67092&#8242;,3&#8242;-\u73af\u78f7\u9178\u672b\u7aef\u7684RNA\u7247\u6bb5\uff0c\u5728\u5316\u5b66\u6027\u8d28\u4e0a\u4e0e\u88abIRE1\u5207\u5272\u7684XBP1\u7247\u6bb5\u5b8c\u5168\u4e00\u81f4\u3002\u8fd9\u79cd\u5927\u89c4\u6a21\u7684\u975e\u5178\u578bRNA\u672b\u7aef\u79ef\u7d2f\u53ef\u80fd\u4f1a\u88ab\u7ec6\u80de\u611f\u77e5\u4e3a\u4e00\u79cd\u201c\u5047\u6027\u201d\u7684ER\u5e94\u6fc0\u4fe1\u53f7\uff0c\u6216\u8005\u5b9e\u9645\u4e0a\u52ab\u6301\u4e86\u7ec6\u80de\u5185\u7684RtcB\u5e93\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">5.2 MED12\u8c03\u63a7RtcB\u8868\u8fbe\u7684\u5047\u8bbe<\/h3>\n\n\n\n<p>\u5982\u679c\u62a5\u544a\u7cfb\u7edf\u7684\u8fd0\u884c\u9700\u8981\u9ad8\u6c34\u5e73\u7684RtcB\u6d3b\u6027\uff0c\u90a3\u4e48\u7ec6\u80de\u53ef\u80fd\u9700\u8981\u4e0a\u8c03 <em>RTCB<\/em> \u57fa\u56e0\u7684\u8f6c\u5f55\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>MED12\u4f5c\u4e3a <em>RTCB<\/em> \u7684\u8f6c\u5f55\u6fc0\u6d3b\u5b50\uff1a<\/strong> \u5c3d\u7ba1 <em>RTCB<\/em> \u901a\u5e38\u88ab\u8ba4\u4e3a\u662f\u770b\u5bb6\u57fa\u56e0\uff0c\u4f46\u5728\u9ad8\u8d1f\u8377\uff08\u5982\u5927\u91cf\u8f6c\u67d3\u62a5\u544a\u8d28\u7c92\uff09\u4e0b\uff0c\u5176\u8868\u8fbe\u53ef\u80fd\u53d7\u5230\u8c03\u63a7\u3002\u5982\u679cMED12\u662f <em>RTCB<\/em> \u57fa\u56e0\u672c\u8eab\u8f6c\u5f55\u6240\u5fc5\u9700\u7684\uff08\u4f5c\u4e3a\u901a\u7528\u6216\u7279\u5f02\u6027\u5171\u6fc0\u6d3b\u56e0\u5b50\uff09\uff0c\u90a3\u4e48MED12\u7684\u6572\u9664\u5c06\u5bfc\u81f4RtcB\u86cb\u767d\u6c34\u5e73\u4e0b\u964d\u3002<\/li>\n\n\n\n<li><strong>\u8bc1\u636e\u94fe\uff1a<\/strong> \u7f3a\u4e4fRtcB -> \u8fde\u63a5\u6548\u7387\u4e0b\u964d -> GFP\u62a5\u544a\u57fa\u56e0\u65e0\u6cd5\u5f62\u6210 -> \u8367\u5149\u964d\u4f4e\u3002\u8fd9\u5b8c\u7f8e\u89e3\u91ca\u4e86\u4e3a\u4f55MED12\u662f\u6fc0\u6d3b\u56e0\u5b50\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">5.3 \u7ec6\u80de\u7a33\u6001\u4e0e\u4ee3\u8c22<\/h3>\n\n\n\n<p>MED12\u548cCDK8\u5728\u8c03\u63a7\u7ec6\u80de\u4ee3\u8c22\u548c\u5e94\u5bf9\u8425\u517b\u538b\u529b\u4e2d\u626e\u6f14\u91cd\u8981\u89d2\u8272 <sup>27<\/sup>\u3002RNA\u8fde\u63a5\u662f\u4e00\u4e2a\u8017\u80fd\u8fc7\u7a0b\uff08\u9700\u8981GTP\uff09\u3002MED12\u7f3a\u5931\u5bfc\u81f4\u7684\u7ec6\u80de\u4ee3\u8c22\u72b6\u6001\u7d0a\u4e71\uff08\u5982ATP\/GTP\u6c34\u5e73\u6ce2\u52a8\u6216\u7ffb\u8bd1\u673a\u5668\u7684\u4e0b\u8c03\uff09\u53ef\u80fd\u95f4\u63a5\u5f71\u54cd\u4e86RtcB\uff08\u4e00\u79cdGTP\u4f9d\u8d56\u6027\u9176\uff09\u7684\u50ac\u5316\u6548\u7387\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">\u7b2c\u516d\u7ae0 \u7efc\u5408\u5206\u6790\uff1a\u4e3a\u4ec0\u4e48\u662fMED12\u30018\u30016\u30014\u300110\uff1f<\/h2>\n\n\n\n<p>\u5728\u6210\u767e\u4e0a\u5343\u4e2a\u6f5c\u5728\u7684\u57fa\u56e0\u4e2d\uff0c\u7b5b\u9009\u7279\u5f02\u6027\u5730\u5bcc\u96c6\u4e86\u8fd9\u4e94\u4e2a\u57fa\u56e0\uff0c\u8fd9\u7edd\u975e\u5de7\u5408\u3002\u5b83\u4eec\u6784\u6210\u4e86\u4e00\u4e2a\u529f\u80fd\u6027\u7684\u5c42\u7ea7\u7ed3\u6784\uff1a<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">6.1 \u6838\u5fc3\u5c42\u7ea7\uff08Core Layer\uff09\uff1aMED6, MED8, MED4, MED10<\/h3>\n\n\n\n<p>\u8fd9\u56db\u4e2a\u4e9a\u57fa\u7684\u51fa\u73b0\u4ee3\u8868\u4e86**\u201c\u8f6c\u5f55\u8bb8\u53ef\u201d\uff08Transcriptional Permissiveness\uff09**\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u5fc5\u8981\u6027\uff1a<\/strong> \u5b83\u4eec\u662fMediator\u5934\u90e8\u548c\u4e2d\u90e8\u6a21\u5757\u7684\u9aa8\u67b6\u3002\u6ca1\u6709\u5b83\u4eec\uff0cPol II\u6839\u672c\u65e0\u6cd5\u88ab\u62db\u52df\u5230\u542f\u52a8\u5b50\u3002<\/li>\n\n\n\n<li><strong>\u8868\u578b\u89e3\u91ca\uff1a<\/strong> \u5b83\u4eec\u7684\u6572\u9664\u5bfc\u81f4\u62a5\u544a\u57fa\u56e0\u7684mRNA\u51e0\u4e4e\u4e0d\u4ea7\u751f\u3002\u6ca1\u6709\u5e95\u7269\uff0c\u81ea\u7136\u6ca1\u6709\u8367\u5149\u3002\u8fd9\u662f\u6700\u57fa\u7840\u7684\u5c42\u7ea7\uff0c\u7c7b\u4f3c\u4e8e\u7535\u6e90\u5f00\u5173\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">6.2 \u8c03\u63a7\u5c42\u7ea7\uff08Regulatory Layer\uff09\uff1aMED12<\/h3>\n\n\n\n<p>MED12\u7684\u51fa\u73b0\u4ee3\u8868\u4e86**\u201c\u8f6c\u5f55\u8d28\u91cf\u4e0e\u52a0\u5de5\u8026\u5408\u201d\uff08Transcriptional Quality and Processing Coupling\uff09**\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u7279\u5f02\u6027\uff1a<\/strong> \u5e76\u975e\u6240\u6709Mediator\u4e9a\u57fa\u90fd\u4e0d\u4ec5\u5f71\u54cd\u8f6c\u5f55\u91cf\uff0c\u8fd8\u5f71\u54cd\u8f6c\u5f55\u7684\u201c\u8d28\u201d\uff08\u5ef6\u4f38\u6027\u3001\u6682\u505c\uff09\u3002MED12\u4f5c\u4e3a\u6fc0\u9176\u6a21\u5757\u7684\u951a\uff0c\u7279\u5f02\u6027\u5730\u8054\u7cfb\u4e86\u5ef6\u4f38\u56e0\u5b50\uff08SEC\uff09\u3002<\/li>\n\n\n\n<li><strong>\u5173\u952e\u63a8\u8bba\uff1a<\/strong> \u65e2\u7136MED12\u4e0e\u6838\u5fc3\u4e9a\u57fa\u4e00\u540c\u51fa\u73b0\uff0c\u8bf4\u660e\u8be5\u62a5\u544a\u7cfb\u7edf\u4e0d\u4ec5\u4ec5\u9700\u8981\u201c\u6709\u8f6c\u5f55\u201d\uff0c\u8fd8\u9700\u8981\u201c\u9ad8\u8d28\u91cf\u7684\u5ef6\u4f38\u201d\u3002Twister\/Rzb\u6838\u9176\u5bf9\u8f6c\u5f55\u73af\u5883\u7684\u6781\u5ea6\u654f\u611f\u6027\uff0c\u4f7f\u5f97\u666e\u901a\u7684\u8f6c\u5f55\uff08\u5982\u679c\u7f3a\u4e4fMED12\u63d0\u4f9b\u7684\u5ef6\u4f38\u8f85\u52a9\uff09\u4e0d\u8db3\u4ee5\u652f\u6301\u6838\u9176\u7684\u9ad8\u6548\u526a\u5207\u3002\u56e0\u6b64\uff0cMED12\u7684\u7f3a\u5931\u867d\u7136\u53ef\u80fd\u53ea\u90e8\u5206\u964d\u4f4e\u8f6c\u5f55\u8d77\u59cb\uff0c\u4f46\u5374\u56e0\u7834\u574f\u4e86\u5ef6\u4f38\u52a8\u529b\u5b66\u800c\u5bfc\u81f4\u6838\u9176\u6d3b\u6027\u53ca\u540e\u7eedRtcB\u8fde\u63a5\u7684<strong>\u5168\u9762\u5d29\u6e83<\/strong>\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">6.3 \u6f5c\u5728\u7684\u53cd\u9988\u56de\u8def<\/h3>\n\n\n\n<p>RtcB\u8fde\u63a5\u4ea7\u751f\u7684\u73af\u72b6RNA\u6216\u7279\u5b9a\u7ed3\u6784RNA\u53ef\u80fd\u5728\u7ec6\u80de\u5185\u5177\u6709\u8f83\u957f\u7684\u534a\u8870\u671f\u3002Mediator\u53ef\u80fd\u8fd8\u53c2\u4e0e\u4e86\u5bf9\u8fd9\u4e9b\u7279\u5b9a\u7ed3\u6784RNA\u7684\u7a33\u5b9a\u6027\u8c03\u63a7\uff0c\u6216\u8005\u901a\u8fc7\u4e0eRNA\u7ed3\u5408\u86cb\u767d\uff08\u5982DDX1\uff09\u7684\u4e92\u4f5c\uff0c\u6784\u5efa\u4e86\u4e00\u4e2a\u6709\u5229\u4e8e\u975e\u5178\u578bRNA\u52a0\u5de5\u7684\u6838\u5185\u5fae\u73af\u5883\uff08Nuclear Compartment\uff09\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">\u7b2c\u4e03\u7ae0 \u7ed3\u8bba\u4e0e\u5c55\u671b<\/h2>\n\n\n\n<p>\u672c\u7814\u7a76\u62a5\u544a\u901a\u8fc7\u5bf9\u7b5b\u9009\u6570\u636e\u7684\u6df1\u5ea6\u6316\u6398\u548c\u6587\u732e\u7efc\u8ff0\uff0c\u63ed\u793a\u4e86Mediator\u590d\u5408\u4f53\u5728RtcB\u4f9d\u8d56\u578bSplit-GFP\u62a5\u544a\u7cfb\u7edf\u4e2d\u7684\u591a\u7ef4\u8c03\u63a7\u673a\u5236\u3002<\/p>\n\n\n\n<p><strong>\u6838\u5fc3\u7ed3\u8bba\uff1a<\/strong><\/p>\n\n\n\n<ol start=\"1\" class=\"wp-block-list\">\n<li><strong>MED6, 8, 4, 10<\/strong>\u4f5c\u4e3aMediator\u7684\u6838\u5fc3\u7ed3\u6784\u6210\u5206\uff0c\u4e3b\u8981\u901a\u8fc7\u4fdd\u969cPIC\u7684\u7ec4\u88c5\u548cPol II\u7684\u62db\u52df\uff0c\u786e\u4fdd\u62a5\u544a\u57fa\u56e0\u7684<strong>\u57fa\u7840\u8f6c\u5f55\u6c34\u5e73<\/strong>\u3002<\/li>\n\n\n\n<li><strong>MED12<\/strong>\u4f5c\u4e3a\u6fc0\u9176\u6a21\u5757\u7684\u5173\u952e\u6210\u5458\uff0c\u901a\u8fc7\u62db\u52dfP-TEFb\/SEC\u590d\u5408\u7269\uff0c\u7cbe\u7ec6\u8c03\u63a7Pol II\u7684<strong>\u8f6c\u5f55\u5ef6\u4f38\u901f\u7387\u548c\u6682\u505c\u91ca\u653e<\/strong>\u3002\u8fd9\u79cd\u52a8\u529b\u5b66\u63a7\u5236\u5bf9\u4e8e\u5171\u8f6c\u5f55\u8fc7\u7a0b\u4e2d<strong>Twister\u548cRzb\u6838\u9176\u7684\u6b63\u786e\u6298\u53e0\u4e0e\u81ea\u526a\u5207<\/strong>\u81f3\u5173\u91cd\u8981\u3002<\/li>\n\n\n\n<li><strong>RtcB\u8fde\u63a5\u6548\u7387<\/strong>\u662f\u8f6c\u5f55\u5ef6\u4f38\u8d28\u91cf\u7684\u76f4\u63a5\u4e0b\u6e38\u8bfb\u51fa\uff08Readout\uff09\u3002MED12\u7f3a\u5931\u5bfc\u81f4\u7684\u5ef6\u4f38\u7f3a\u9677\u4f1a\u4ea7\u751f\u672a\u526a\u5207\u6216\u9519\u8bef\u6298\u53e0\u7684\u524d\u4f53RNA\uff0c\u4ece\u800c\u963b\u65adRtcB\u7684\u8fde\u63a5\u5e95\u7269\u4f9b\u7ed9\u3002<\/li>\n\n\n\n<li>\u8be5\u7b5b\u9009\u7ed3\u679c\u5f3a\u8c03\u4e86<strong>\u8f6c\u5f55\u673a\u5668\u4e0eRNA\u52a0\u5de5\u673a\u5668\uff08\u7279\u522b\u662f\u6838\u9176\u548cRtcB\uff09\u4e4b\u95f4\u5b58\u5728\u7d27\u5bc6\u7684\u52a8\u529b\u5b66\u8026\u5408<\/strong>\u3002<\/li>\n<\/ol>\n\n\n\n<p><strong>\u672a\u6765\u7814\u7a76\u5efa\u8bae\uff1a<\/strong><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u9a8c\u8bc1\u52a8\u529b\u5b66\u6a21\u578b\uff1a<\/strong> \u4f7f\u7528GRO-seq\u6216PRO-seq\u6280\u672f\u68c0\u6d4bMED12\u6572\u9664\u7ec6\u80de\u4e2d\u62a5\u544a\u57fa\u56e0\u4e0a\u7684Pol II\u5206\u5e03\uff0c\u89c2\u5bdf\u662f\u5426\u5b58\u5728\u5f02\u5e38\u7684\u6682\u505c\u6216\u5806\u79ef\u3002<\/li>\n\n\n\n<li><strong>\u68c0\u6d4b\u4e2d\u95f4\u4f53\uff1a<\/strong> \u4f7f\u7528Northern Blot\u6216RT-PCR\u68c0\u6d4b\u672a\u526a\u5207\u524d\u4f53\uff08Precursor\uff09\u4e0e\u526a\u5207\u540e\/\u8fde\u63a5\u540e\u4ea7\u7269\u7684\u6bd4\u4f8b\uff0c\u4ee5\u533a\u5206\u662f\u8f6c\u5f55\u603b\u91cf\u51cf\u5c11\u8fd8\u662f\u52a0\u5de5\u6548\u7387\u964d\u4f4e\u3002<\/li>\n\n\n\n<li><strong>RtcB\u6c34\u5e73\u68c0\u6d4b\uff1a<\/strong> 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has-alpha-channel-opacity\"\/>\n\n\n\n<p>\u53c2\u8003\u6587\u732e\u5f15\u7528\u8bf4\u660e\uff1a<\/p>\n\n\n\n<p>\u672c\u62a5\u544a\u4e2d\u5f15\u7528\u7684\u5173\u952e\u4e8b\u5b9e\u548c\u6570\u636e\u652f\u6301\u6765\u81ea\u4ee5\u4e0b\u6587\u732e\u7f16\u53f7\uff1a<\/p>\n\n\n\n<p>5 \u7b49\u3002\u6240\u6709\u7ed3\u6784\u63cf\u8ff0\u3001\u751f\u5316\u673a\u5236\u53ca\u529f\u80fd\u5173\u8054\u5747\u57fa\u4e8e\u63d0\u4f9b\u7684\u79d1\u7814\u7247\u6bb5\u8fdb\u884c\u4e86\u903b\u8f91\u63a8\u6f14\u4e0e\u6574\u5408\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>\u5f15\u7528\u7684\u8457\u4f5c<\/strong><\/h4>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Multiscale In Silico Study of the Mechanism of Activation of the RtcB Ligase by the PTP1B Phosphatase | Journal of Chemical Information and Modeling &#8211; ACS Publications, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.jcim.3c01600\">https:\/\/pubs.acs.org\/doi\/10.1021\/acs.jcim.3c01600<\/a><\/li>\n\n\n\n<li>Insights into the structure and function of the RNA ligase RtcB &#8211; PMC &#8211; PubMed Central, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC10630183\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC10630183\/<\/a><\/li>\n\n\n\n<li>Light-controlled twister ribozyme with single-molecule detection resolves RNA function in time and space | PNAS, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.pnas.org\/doi\/abs\/10.1073\/pnas.2003425117\">https:\/\/www.pnas.org\/doi\/abs\/10.1073\/pnas.2003425117<\/a><\/li>\n\n\n\n<li>Metals induce transient folding and activation of the Twister ribozyme &#8211; PubMed Central, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC5605428\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC5605428\/<\/a><\/li>\n\n\n\n<li>The complex structure and function of Mediator &#8211; PMC, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6130968\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6130968\/<\/a><\/li>\n\n\n\n<li>Mediator (coactivator) &#8211; Wikipedia, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/en.wikipedia.org\/wiki\/Mediator_(coactivator)\">https:\/\/en.wikipedia.org\/wiki\/Mediator_(coactivator)<\/a><\/li>\n\n\n\n<li>Structure and Functions of the Mediator Complex, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"http:\/\/www.protein.bio.msu.ru\/biokhimiya\/contents\/v83\/pdf\/BCM0423.pdf\">http:\/\/www.protein.bio.msu.ru\/biokhimiya\/contents\/v83\/pdf\/BCM0423.pdf<\/a><\/li>\n\n\n\n<li>Evidence that Mediator is essential for Pol II transcription, but is not a &#8211; required component of the preinitiation complex in vivo &#8211; eLife, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/elifesciences.org\/articles\/28447v1.pdf\">https:\/\/elifesciences.org\/articles\/28447v1.pdf<\/a><\/li>\n\n\n\n<li>The Mediator complex as a master regulator of transcription by RNA polymerase II &#8211; PMC, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC9207880\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC9207880\/<\/a><\/li>\n\n\n\n<li>Origins and Activity of the Mediator Complex &#8211; PMC &#8211; NIH, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3207015\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3207015\/<\/a><\/li>\n\n\n\n<li>Role of Mediator in Regulating Pol II Elongation and Nucleosome Displacement in Saccharomyces cerevisiae &#8211; PMC &#8211; PubMed Central, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3338273\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3338273\/<\/a><\/li>\n\n\n\n<li>MED12 Gene &#8211; Mediator Complex Subunit 12 &#8211; GeneCards, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.genecards.org\/cgi-bin\/carddisp.pl?gene=MED12\">https:\/\/www.genecards.org\/cgi-bin\/carddisp.pl?gene=MED12<\/a><\/li>\n\n\n\n<li>HIF1A employs CDK8-Mediator to stimulate RNAPII elongation &#8211; PMC, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3681429\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3681429\/<\/a><\/li>\n\n\n\n<li>CDK8 is a positive regulator of transcriptional elongation within the serum response network, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC2920286\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC2920286\/<\/a><\/li>\n\n\n\n<li>Precise Maps of RNA Polymerase Reveal How Promoters Direct Initiation and Pausing | Request PDF &#8211; ResearchGate, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.researchgate.net\/publication\/235691213_Precise_Maps_of_RNA_Polymerase_Reveal_How_Promoters_Direct_Initiation_and_Pausing\">https:\/\/www.researchgate.net\/publication\/235691213_Precise_Maps_of_RNA_Polymerase_Reveal_How_Promoters_Direct_Initiation_and_Pausing<\/a><\/li>\n\n\n\n<li>Flanking Sequence Cotranscriptionally Regulates Twister Ribozyme Activity &#8211; NSF PAR, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/par.nsf.gov\/servlets\/purl\/10491321\">https:\/\/par.nsf.gov\/servlets\/purl\/10491321<\/a><\/li>\n\n\n\n<li>A roadmap for ribosomal RNA folding and assembly during transcription &#8211; PMC, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC8526401\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC8526401\/<\/a><\/li>\n\n\n\n<li>Gene Regulation by a Kinetic Riboswitch with Negative Feedback Loop &#8211; ACS Publications, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.jpcb.4c06581\">https:\/\/pubs.acs.org\/doi\/10.1021\/acs.jpcb.4c06581<\/a><\/li>\n\n\n\n<li>Ligand Modulates Cross-Coupling between Riboswitch Folding and Transcriptional Pausing | Request PDF &#8211; ResearchGate, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.researchgate.net\/publication\/336988505_Ligand_Modulates_Cross-Coupling_between_Riboswitch_Folding_and_Transcriptional_Pausing\">https:\/\/www.researchgate.net\/publication\/336988505_Ligand_Modulates_Cross-Coupling_between_Riboswitch_Folding_and_Transcriptional_Pausing<\/a><\/li>\n\n\n\n<li>Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6554452\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6554452\/<\/a><\/li>\n\n\n\n<li>Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit &#8211; PMC, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3288850\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3288850\/<\/a><\/li>\n\n\n\n<li>Long range chromatin organization: a new layer in splicing regulation? &#8211; PMC &#8211; NIH, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC4574877\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC4574877\/<\/a><\/li>\n\n\n\n<li>RTCB Gene | WikiCrow AI, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/wikicrow.ai\/RTCB\">https:\/\/wikicrow.ai\/RTCB<\/a><\/li>\n\n\n\n<li>Co-transcriptional regulation of alternative pre-mRNA splicing &#8211; PMC &#8211; NIH, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3371144\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3371144\/<\/a><\/li>\n\n\n\n<li>Understanding the Unfolded Protein Response (UPR) Pathway: Insights into Neuropsychiatric Disorders and Therapeutic Potentials &#8211; PMC &#8211; PubMed Central, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC10902702\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC10902702\/<\/a><\/li>\n\n\n\n<li>E3 ligase substrate adaptor SPOP fine-tunes the UPR of pancreatic \u03b2 cells &#8211; PMC, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC11789638\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC11789638\/<\/a><\/li>\n\n\n\n<li>The CDK8 kinase module: A novel player in the transcription of translation initiation and ribosomal genes &#8211; PMC &#8211; PubMed Central, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC11742111\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC11742111\/<\/a><\/li>\n\n\n\n<li>Effect of Structure in a Long Target RNA on Ribozyme Cleavage Efficiency | Nucleic Acids Research | Oxford Academic, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/academic.oup.com\/nar\/article\/25\/24\/4985\/1748375\">https:\/\/academic.oup.com\/nar\/article\/25\/24\/4985\/1748375<\/a><\/li>\n\n\n\n<li>A circular split nanoluciferase reporter for validating and screening putative internal ribosomal entry site elements &#8211; NIH, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC11482608\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC11482608\/<\/a><\/li>\n\n\n\n<li>(PDF) Ribozyme-activated mRNA trans-ligation enables large gene delivery to treat muscular dystrophies &#8211; ResearchGate, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.researchgate.net\/publication\/385824320_Ribozyme-activated_mRNA_trans-ligation_enables_large_gene_delivery_to_treat_muscular_dystrophies\">https:\/\/www.researchgate.net\/publication\/385824320_Ribozyme-activated_mRNA_trans-ligation_enables_large_gene_delivery_to_treat_muscular_dystrophies<\/a><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>\u672c\u7814\u7a76\u901a\u8fc7\u4e00\u4e2a\u7cbe\u5999\u7684RtcB\u4f9d\u8d56\u578bSplit-GFP\u62a5\u544a\u7cfb\u7edf\u8fdb\u884cCRISPR\u7b5b\u9009\uff0c\u610f\u5916\u63ed\u793a\u4e86Mediator\u590d\u5408\u4f53\u6838\u5fc3\u4e9a\u57fa\uff08MED12, 8, 6, 4, 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