{"id":13622,"date":"2025-12-13T19:52:19","date_gmt":"2025-12-13T11:52:19","guid":{"rendered":"https:\/\/fanyiming.life\/?p=13622"},"modified":"2025-12-21T01:02:19","modified_gmt":"2025-12-20T17:02:19","slug":"mediator-%e5%a4%8d%e5%90%88%e7%89%a9%e6%b7%b1%e5%ba%a6%e7%a0%94%e7%a9%b6%e6%8a%a5%e5%91%8a%ef%bc%9a%e4%bb%8e%e7%bb%93%e6%9e%84%e5%8a%a8%e5%8a%9b%e5%ad%a6%e5%88%b0%e8%bd%ac%e5%bd%95%e4%b8%8e-rna","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=13622","title":{"rendered":"Mediator \u590d\u5408\u7269\u6df1\u5ea6\u7814\u7a76\u62a5\u544a\uff1a\u4ece\u7ed3\u6784\u52a8\u529b\u5b66\u5230\u8f6c\u5f55\u4e0e RNA \u8fde\u63a5\u7684\u4ea4\u4e92\u673a\u5236"},"content":{"rendered":"\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>By Google Gemini-3-pro-preview Deep Research.<\/p>\n<\/blockquote>\n\n\n\n<h2 class=\"wp-block-heading\">1. \u6982\u8ff0\u4e0e\u6838\u5fc3\u91cd\u8981\u6027.<\/h2>\n\n\n\n<p>\u5728\u771f\u6838\u751f\u7269\u7684\u57fa\u56e0\u8868\u8fbe\u8c03\u63a7\u7f51\u7edc\u4e2d\uff0c<strong>Mediator \u590d\u5408\u7269\uff08\u4e2d\u4ecb\u4f53\u590d\u5408\u7269\uff09<\/strong> \u5360\u636e\u7740\u7edd\u5bf9\u7684\u6838\u5fc3\u5730\u4f4d\u3002\u4f5c\u4e3a\u4e00\u4e2a\u5e9e\u5927\u3001\u8fdb\u5316\u4e0a\u9ad8\u5ea6\u4fdd\u5b88\u7684\u591a\u86cb\u767d\u673a\u5668\uff0cMediator \u5e76\u4e0d\u4ec5\u4ec5\u662f\u8fde\u63a5\u8f6c\u5f55\u56e0\u5b50\uff08Transcription Factors, TFs\uff09\u4e0e RNA \u805a\u5408\u9176 II\uff08RNA Polymerase II, Pol II\uff09\u7684\u7b80\u5355\u6865\u6881\uff0c\u5b83\u5b9e\u9645\u4e0a\u662f\u7ec6\u80de\u6838\u5185\u6574\u5408\u5404\u79cd\u4fe1\u53f7\u3001\u91cd\u5851\u67d3\u8272\u8d28\u7ed3\u6784\u3001\u9a71\u52a8\u76f8\u5206\u79bb\uff08Phase Separation\uff09\u5e76\u534f\u8c03\u8f6c\u5f55\u540e\u52a0\u5de5\u7684\u201c\u4e3b\u63a7\u5904\u7406\u5668\u201d\u3002<\/p>\n\n\n\n<p>\u4ece\u9175\u6bcd\u5230\u4eba\u7c7b\uff0cMediator \u7684\u7ed3\u6784\u548c\u529f\u80fd\u5c55\u73b0\u51fa\u4e86\u60ca\u4eba\u7684\u590d\u6742\u6027\u3002\u5b83\u7531 20 \u5230 30 \u4e2a\u4e9a\u57fa\u7ec4\u6210\uff0c\u5206\u5b50\u91cf\u8d85\u8fc7 1 MDa\uff08\u5146\u9053\u5c14\u987f\uff09\uff0c\u5728\u7ed3\u6784\u4e0a\u88ab\u5212\u5206\u4e3a\u56db\u4e2a\u529f\u80fd\u6a21\u5757\uff1a<strong>\u5934\u90e8\uff08Head\uff09<\/strong>\u3001<strong>\u4e2d\u90e8\uff08Middle\uff09<\/strong>\u3001<strong>\u5c3e\u90e8\uff08Tail\uff09<\/strong> \u4ee5\u53ca\u53ef\u89e3\u79bb\u7684 <strong>\u6fc0\u9176\u6a21\u5757\uff08Kinase Module\/CDK8 Module\uff09<\/strong> <sup>1<\/sup>\u3002\u8fc7\u53bb\u4e09\u5341\u5e74\u7684\u7814\u7a76\u5df2\u7ecf\u786e\u7acb\u4e86 Mediator \u5728\u8f6c\u5f55\u9884\u8d77\u59cb\u590d\u5408\u7269\uff08Pre-Initiation Complex, PIC\uff09\u7ec4\u88c5\u4e2d\u7684\u5fc5\u8981\u6027\uff0c\u800c\u6700\u65b0\u7684\u7814\u7a76\u524d\u6cbf\u5219\u63ed\u793a\u4e86\u5b83\u5728\u8d85\u7ea7\u589e\u5f3a\u5b50\uff08Super-Enhancers\uff09\u5f62\u6210\u3001\u67d3\u8272\u8d28\u73af\uff08Chromatin Looping\uff09\u7a33\u5b9a\u4ee5\u53ca\u4e0e RNA \u52a0\u5de5\u673a\u5668\uff08\u5982 RNA \u8fde\u63a5\u9176 RTCB\uff09\u4ea4\u4e92\u4e2d\u7684\u65b0\u5174\u89d2\u8272\u3002<\/p>\n\n\n\n<p>\u672c\u62a5\u544a\u5c06\u5bf9 Mediator \u590d\u5408\u7269\u8fdb\u884c\u8be6\u5c3d\u7684\u5256\u6790\uff0c\u6db5\u76d6\u5176\u5206\u5b50\u7ed3\u6784\u3001\u5173\u952e\u7ec4\u5206\u7684\u529f\u80fd\u673a\u5236\u3001\u76f8\u5206\u79bb\u751f\u7269\u7269\u7406\u5b66\u7279\u6027\uff0c\u5e76\u91cd\u70b9\u63a2\u8ba8\u7528\u6237\u5173\u6ce8\u7684 <strong>Mediator \u4e0e RNA \u8fde\u63a5\uff08RNA Ligation\uff09\u53ca RTCB \u86cb\u767d<\/strong> \u4e4b\u95f4\u9519\u7efc\u590d\u6742\u7684\u5173\u8054\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">2. Mediator \u590d\u5408\u7269\u7684\u6a21\u5757\u5316\u7ed3\u6784\u4e0e\u7ec4\u88c5<\/h2>\n\n\n\n<p>Mediator \u7684\u7ed3\u6784\u89e3\u6790\u4e00\u76f4\u662f\u7ed3\u6784\u751f\u7269\u5b66\u9886\u57df\u7684\u5de8\u5927\u6311\u6218\uff0c\u56e0\u4e3a\u5b83\u5177\u6709\u9ad8\u5ea6\u7684\u6784\u8c61\u7075\u6d3b\u6027\u548c\u5927\u91cf\u7684\u5185\u5728\u65e0\u5e8f\u533a\u57df\uff08Intrinsically Disordered Regions, IDRs\uff09\u3002\u901a\u8fc7\u51b7\u51bb\u7535\u955c\uff08Cryo-EM\uff09\u548c\u4ea4\u8054\u8d28\u8c31\uff08CXMS\uff09\u6280\u672f\uff0c\u79d1\u5b66\u5bb6\u4eec\u7ec8\u4e8e\u63ed\u793a\u4e86\u5176\u7cbe\u7ec6\u7684\u6a21\u5757\u5316\u7ec4\u88c5\u65b9\u5f0f\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">2.1 \u6838\u5fc3\u590d\u5408\u7269\uff08Core Mediator\uff09\uff1a\u8f6c\u5f55\u7684\u6267\u884c\u8005<\/h3>\n\n\n\n<p>\u6838\u5fc3 Mediator \u7531\u5934\u90e8\u3001\u4e2d\u90e8\u548c\u5c3e\u90e8\u6a21\u5757\u7ec4\u6210\uff0c\u76f4\u63a5\u4e0e Pol II \u548c\u901a\u7528\u8f6c\u5f55\u56e0\u5b50\uff08\u5982 TFIID, TFIIH\uff09\u76f8\u4e92\u4f5c\u7528\uff0c\u8d1f\u8d23 PIC \u7684\u7a33\u5b9a\u548c\u6fc0\u6d3b\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">2.1.1 \u5934\u90e8\u6a21\u5757 (Head Module)<\/h4>\n\n\n\n<p>\u5934\u90e8\u6a21\u5757\u662f Mediator \u4e0e Pol II \u7ed3\u5408\u7684\u6700\u4e3b\u8981\u754c\u9762\uff0c\u4e5f\u662f\u7ef4\u6301\u7ec6\u80de\u751f\u5b58\u6700\u4e0d\u53ef\u6216\u7f3a\u7684\u90e8\u5206\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u7ed3\u6784\u7279\u5f81\uff1a<\/strong> \u5934\u90e8\u6a21\u5757\u5305\u542b\u88ab\u79f0\u4e3a\u201c\u624b\u81c2\uff08Arm\uff09\u201d\u548c\u201c\u4e0b\u989a\uff08Jaws\uff09\u201d\u7684\u7ed3\u6784\u57df\u3002\u201c\u624b\u81c2\u201d\u4e3b\u8981\u7531 <strong>MED17<\/strong>\u3001<strong>MED6<\/strong> \u548c <strong>MED8<\/strong> \u7684\u87ba\u65cb\u675f\u6784\u6210\uff0c\u5b83\u662f\u6355\u6349 Pol II C \u672b\u7aef\u7ed3\u6784\u57df\uff08CTD\uff09\u7684\u5173\u952e\u90e8\u4f4d <sup>2<\/sup>\u3002<\/li>\n\n\n\n<li><strong>\u5173\u952e\u4e9a\u57fa MED17 (Srb4)\uff1a<\/strong> MED17 \u88ab\u8ba4\u4e3a\u662f\u5934\u90e8\u6a21\u5757\u7684\u652f\u67b6\u3002\u7814\u7a76\u8868\u660e\uff0cMED17 \u7684\u529f\u80fd\u7f3a\u5931\u7a81\u53d8\u4f1a\u5bfc\u81f4\u5168\u57fa\u56e0\u7ec4\u8303\u56f4\u5185 Pol II \u8f6c\u5f55\u7684\u51e0\u4e4e\u5b8c\u5168\u505c\u6b62\uff0c\u5176\u8868\u578b\u4e0e Pol II \u672c\u8eab\u7684\u7f3a\u5931\u6781\u4e3a\u76f8\u4f3c\u3002\u8fd9\u5f3a\u8c03\u4e86 Mediator \u5934\u90e8\u6a21\u5757\u5728\u57fa\u7840\u8f6c\u5f55\u4e2d\u7684\u6838\u5fc3\u5730\u4f4d <sup>3<\/sup>\u3002<\/li>\n\n\n\n<li><strong>Pol II \u7684\u6355\u83b7\uff1a<\/strong> \u5934\u90e8\u6a21\u5757\u7684\u79fb\u52a8\u4e0b\u989a\uff08\u7531 <strong>MED18<\/strong> \u548c <strong>MED20<\/strong> \u7ec4\u6210\uff09\u53ef\u4ee5\u7075\u6d3b\u8c03\u6574\u6784\u8c61\uff0c\u50cf\u94b3\u5b50\u4e00\u6837\u56fa\u5b9a DNA \u6a21\u677f\u4e0e Pol II \u7684\u7ed3\u5408\u69fd\uff0c\u4ece\u800c\u4fc3\u8fdb\u8f6c\u5f55\u8d77\u59cb\u6ce1\u7684\u5f62\u6210 <sup>2<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">2.1.2 \u4e2d\u90e8\u6a21\u5757 (Middle Module)<\/h4>\n\n\n\n<p>\u4e2d\u90e8\u6a21\u5757\u8d77\u5230\u4e86\u8fde\u63a5\u5934\u90e8\u548c\u5c3e\u90e8\u7684\u7ed3\u6784\u67a2\u7ebd\u4f5c\u7528\uff0c\u540c\u65f6\u4e5f\u662f\u8c03\u63a7\u4fe1\u53f7\u4f20\u9012\u7684\u5173\u952e\u533a\u57df\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6838\u5fc3\u652f\u67b6 MED14 (Rgr1)\uff1a<\/strong> \u5c3d\u7ba1\u5728\u67d0\u4e9b\u5206\u7c7b\u4e2d MED14 \u88ab\u5f52\u7c7b\u4e3a\u5c3e\u90e8\u6216\u72ec\u7acb\u4e9a\u57fa\uff0c\u4f46\u5b83\u5b9e\u9645\u4e0a\u662f\u8d2f\u7a7f\u5934\u90e8\u3001\u4e2d\u90e8\u548c\u5c3e\u90e8\u7684\u201c\u810a\u6881\uff08Backbone\uff09\u201d\u3002MED14 \u901a\u8fc7\u5176\u6781\u957f\u7684\u5ef6\u4f38\u7ed3\u6784\uff0c\u7269\u7406\u4e0a\u8fde\u63a5\u4e86\u4e09\u4e2a\u6a21\u5757\u3002MED14 \u7684\u7f3a\u5931\u4f1a\u5bfc\u81f4 Mediator \u590d\u5408\u7269\u89e3\u4f53\u6210\u65e0\u529f\u80fd\u7684\u4e9a\u6a21\u5757\uff0c\u5bfc\u81f4\u57fa\u56e0\u8868\u8fbe\u7684\u707e\u96be\u6027\u7d0a\u4e71 <sup>2<\/sup>\u3002<\/li>\n\n\n\n<li><strong>MED1 \u7684\u7279\u6b8a\u5730\u4f4d\uff1a<\/strong> \u5728\u54fa\u4e73\u52a8\u7269\u4e2d\uff0c<strong>MED1<\/strong> \u662f\u4e2d\u90e8\u6a21\u5757\u4e2d\u6700\u53d7\u5173\u6ce8\u7684\u4e9a\u57fa\u3002\u5b83\u901a\u8fc7\u5176\u4e24\u4e2a LXXLL \u57fa\u5e8f\u4e0e\u6838\u53d7\u4f53\uff08\u5982\u96cc\u6fc0\u7d20\u53d7\u4f53 ER\u3001\u7532\u72b6\u817a\u6fc0\u7d20\u53d7\u4f53 TR\uff09\u76f4\u63a5\u7ed3\u5408\u3002\u66f4\u91cd\u8981\u7684\u662f\uff0cMED1 \u542b\u6709\u4e00\u4e2a\u5de8\u5927\u7684\u5185\u5728\u65e0\u5e8f\u533a\uff08IDR\uff09\uff0c\u8fd9\u662f\u9a71\u52a8\u8f6c\u5f55\u51dd\u805a\u4f53\u76f8\u5206\u79bb\u7684\u5173\u952e\u7269\u7406\u57fa\u7840\uff08\u8be6\u89c1\u7b2c 4 \u8282\uff09<sup>5<\/sup>\u3002<\/li>\n\n\n\n<li><strong>Hook \u7ed3\u6784\u57df\uff1a<\/strong> \u4e2d\u90e8\u6a21\u5757\u7684 <strong>MED4<\/strong>\u3001<strong>MED10<\/strong> \u7b49\u4e9a\u57fa\u5f62\u6210\u4e86\u4e00\u4e2a\u88ab\u79f0\u4e3a\u201cHook\u201d\u7684\u7ed3\u6784\uff0c\u8fd9\u662f\u6fc0\u9176\u6a21\u5757\uff08CDK8 Module\uff09\u7ed3\u5408\u5e76\u8c03\u63a7\u6838\u5fc3 Mediator \u7684\u951a\u5b9a\u70b9 <sup>2<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">2.1.3 \u5c3e\u90e8\u6a21\u5757 (Tail Module)<\/h4>\n\n\n\n<p>\u5c3e\u90e8\u6a21\u5757\u662f Mediator \u4e2d\u53d8\u5f02\u6700\u5927\u3001\u8fdb\u5316\u4e0a\u76f8\u5bf9\u591a\u6837\u5316\u7684\u90e8\u5206\u3002\u5b83\u662f\u57fa\u56e0\u7279\u5f02\u6027\u8f6c\u5f55\u56e0\u5b50\uff08Activators\uff09\u7684\u4e3b\u8981\u505c\u6cca\u6e2f\u53e3\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u7279\u5f02\u6027\u8bc6\u522b\uff1a<\/strong> \u4e0d\u540c\u7684\u8f6c\u5f55\u56e0\u5b50\u901a\u8fc7\u7ed3\u5408\u5c3e\u90e8\u6a21\u5757\u7684\u4e0d\u540c\u4e9a\u57fa\u6765\u62db\u52df Mediator\u3002\u4f8b\u5982\uff0c<strong>MED15<\/strong> \u7684 KIX \u7ed3\u6784\u57df\u662f SREBP\uff08\u8102\u8d28\u4ee3\u8c22\u8c03\u8282\u5b50\uff09\u548c Gcn4\uff08\u6c28\u57fa\u9178\u9965\u997f\u54cd\u5e94\u56e0\u5b50\uff09\u7684\u7ed3\u5408\u9776\u70b9\uff1b\u800c <strong>MED23<\/strong> \u5219\u662f E1A \u548c ELK1\uff08MAPK \u901a\u8def\u4e0b\u6e38\u56e0\u5b50\uff09\u7684\u7ed3\u5408\u9776\u70b9 <sup>6<\/sup>\u3002<\/li>\n\n\n\n<li><strong>\u529f\u80fd\u5206\u5de5\uff1a<\/strong> \u5c3e\u90e8\u6a21\u5757\u7684\u5b58\u5728\u4f7f\u5f97 Mediator \u80fd\u591f\u89e3\u8bfb\u7ec6\u80de\u5185\u6210\u5343\u4e0a\u4e07\u79cd\u4e0d\u540c\u7684\u4fe1\u53f7\u8f93\u5165\uff0c\u5e76\u5c06\u8fd9\u4e9b\u4fe1\u53f7\u8f6c\u5316\u4e3a\u901a\u7528\u7684 Pol II \u62db\u52df\u6307\u4ee4\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">2.2 \u6fc0\u9176\u6a21\u5757 (CDK8 Kinase Module\/CKM)<\/h3>\n\n\n\n<p>\u6fc0\u9176\u6a21\u5757\u7531\u56db\u4e2a\u4e9a\u57fa\u7ec4\u6210\uff1a<strong>CDK8<\/strong>\u3001<strong>Cyclin C (CCNC)<\/strong>\u3001<strong>MED12<\/strong> \u548c <strong>MED13<\/strong>\u3002\u5b83\u4ee5\u53ef\u9006\u7684\u65b9\u5f0f\u4e0e\u6838\u5fc3 Mediator \u7ed3\u5408\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u7a7a\u95f4\u4f4d\u963b\u4e0e\u6291\u5236\uff1a<\/strong> \u65e9\u671f\u9175\u6bcd\u7814\u7a76\u8ba4\u4e3a CKM \u662f\u8f6c\u5f55\u6291\u5236\u56e0\u5b50\uff0c\u56e0\u4e3a\u5176\u7ed3\u5408\u4f1a\u5360\u636e Pol II \u7684\u7ed3\u5408\u4f4d\u70b9\uff08\u7a7a\u95f4\u4f4d\u963b\u6a21\u578b\uff09\u3002<\/li>\n\n\n\n<li><strong>\u8f6c\u5f55\u6fc0\u6d3b\u4e0e\u5ef6\u4f38\uff1a<\/strong> \u5728\u9ad8\u7b49\u771f\u6838\u751f\u7269\u4e2d\uff0cCKM \u7684\u529f\u80fd\u66f4\u52a0\u590d\u6742\u3002\u5b83\u4e0d\u4ec5\u53ef\u4ee5\u901a\u8fc7\u78f7\u9178\u5316 Pol II CTD \u6765\u4fc3\u8fdb\u8f6c\u5f55\u5ef6\u4f38\uff0c\u8fd8\u80fd\u4f5c\u4e3a\u6b63\u5411\u8c03\u8282\u56e0\u5b50\u53c2\u4e0e\u7279\u5b9a\u4fe1\u53f7\u901a\u8def\uff08\u5982 Wnt\/\u03b2-catenin \u901a\u8def\uff09\u7684\u6fc0\u6d3b <sup>8<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n<p><strong>\u8868 1\uff1aMediator \u6fc0\u9176\u6a21\u5757\u5173\u952e\u7ec4\u5206\u529f\u80fd\u8868<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><td><strong>\u4e9a\u57fa (Subunit)<\/strong><\/td><td><strong>\u4eba\u7c7b\u540c\u6e90\u7269\/\u65c1\u7cfb\u540c\u6e90\u7269<\/strong><\/td><td><strong>\u4e3b\u8981\u5206\u5b50\u529f\u80fd<\/strong><\/td><\/tr><\/thead><tbody><tr><td><strong>CDK8<\/strong><\/td><td>CDK19<\/td><td>\u5468\u671f\u86cb\u767d\u4f9d\u8d56\u6027\u6fc0\u9176\uff1b\u78f7\u9178\u5316 Pol II CTD (Ser2\/5) \u53ca\u8f6c\u5f55\u56e0\u5b50\uff08\u5982 Notch, E2F1\uff09\u3002<\/td><\/tr><tr><td><strong>Cyclin C<\/strong><\/td><td>CCNC<\/td><td>\u8c03\u8282\u4e9a\u57fa\uff0c\u6fc0\u6d3b CDK8 \u7684\u6fc0\u9176\u6d3b\u6027\uff1b\u5728\u538b\u529b\u4e0b\u53ef\u7a7f\u68ad\u81f3\u7ec6\u80de\u8d28\u8c03\u8282\u7ebf\u7c92\u4f53\u5206\u88c2\u3002<\/td><\/tr><tr><td><strong>MED12<\/strong><\/td><td>MED12L<\/td><td>\u5de8\u5927\u7684\u652f\u67b6\u86cb\u767d\uff0c\u5305\u88f9 CDK8\/CycC\uff1b\u75be\u75c5\u7a81\u53d8\u70ed\u70b9\uff08\u5b50\u5bab\u808c\u7624\u3001FG \u7efc\u5408\u5f81\uff09\u3002<\/td><\/tr><tr><td><strong>MED13<\/strong><\/td><td>MED13L<\/td><td>\u7ed3\u6784\u201c\u7cfb\u7ef3\uff08Tether\uff09\u201d\uff0c\u7269\u7406\u8fde\u63a5\u6fc0\u9176\u6a21\u5757\u4e0e\u6838\u5fc3 Mediator \u7684\u4e2d\u90e8\u6a21\u5757\uff1b\u8c03\u8282\u4ee3\u8c22\u3002<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">3. \u5173\u952e\u529f\u80fd\u7ec4\u5206\u8be6\u89e3<\/h2>\n\n\n\n<p>\u7528\u6237\u8be2\u95ee\u201c\u5173\u952e\u7ec4\u5206\u662f\u54ea\u51e0\u4e2a\u86cb\u767d\uff1f\u8d77\u5230\u4ec0\u4e48\u4f5c\u7528\uff1f\u201d\uff0c\u4e3a\u4e86\u63d0\u4f9b\u8be6\u5c3d\u7684\u56de\u7b54\uff0c\u6211\u4eec\u4e0d\u4ec5\u8981\u5217\u51fa\u540d\u5355\uff0c\u66f4\u8981\u6df1\u5165\u5176\u5206\u5b50\u673a\u5236\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">3.1 MED1\uff1a\u8d85\u7ea7\u589e\u5f3a\u5b50\u7684\u9a71\u52a8\u8005\u4e0e\u76f8\u5206\u79bb\u6838\u5fc3<\/h3>\n\n\n\n<p><strong>MED1<\/strong> \u662f\u54fa\u4e73\u52a8\u7269 Mediator \u4e2d\u6700\u5927\u7684\u4e9a\u57fa\u4e4b\u4e00\u3002\u5b83\u7684\u6838\u5fc3\u4f5c\u7528\u5728\u4e8e\u4f5c\u4e3a\u6838\u53d7\u4f53\uff08Nuclear Receptors\uff09\u7684\u5171\u6fc0\u6d3b\u56e0\u5b50\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u4f5c\u7528\u673a\u5236\uff1a<\/strong> MED1 \u542b\u6709\u4e00\u6bb5\u6781\u957f\u7684\u5185\u5728\u65e0\u5e8f\u533a\u57df\uff08IDR\uff09\uff0c\u5bcc\u542b\u4e1d\u6c28\u9178\u3002\u8fd9\u6bb5 IDR \u4f7f\u5f97 MED1 \u80fd\u591f\u5728\u8d85\u7ea7\u589e\u5f3a\u5b50\uff08Super-Enhancers\uff09\u533a\u57df\u53d1\u751f <strong>\u6db2-\u6db2\u76f8\u5206\u79bb\uff08Liquid-Liquid Phase Separation, LLPS\uff09<\/strong>\u3002<\/li>\n\n\n\n<li><strong>\u76f8\u5206\u79bb\u7684\u610f\u4e49\uff1a<\/strong> \u901a\u8fc7\u76f8\u5206\u79bb\uff0cMED1 \u5f62\u6210\u5fae\u7c73\u7ea7\u7684\u8f6c\u5f55\u51dd\u805a\u4f53\uff08Transcriptional Condensates\uff09\uff0c\u8fd9\u4e9b\u6db2\u6ef4\u72b6\u7684\u7ed3\u6784\u80fd\u591f\u5bcc\u96c6\u9ad8\u6d53\u5ea6\u7684 Pol II \u548c\u5171\u6fc0\u6d3b\u56e0\u5b50\uff08\u5982 BRD4\uff09\uff0c\u540c\u65f6\u6392\u9664\u6291\u5236\u56e0\u5b50\uff0c\u4ece\u800c\u5b9e\u73b0\u7279\u5b9a\u57fa\u56e0\uff08\u5982 <em>MYC<\/em>, <em>OCT4<\/em> \u7b49\u7ec6\u80de\u547d\u8fd0\u51b3\u5b9a\u57fa\u56e0\uff09\u7684\u7206\u53d1\u5f0f\u8f6c\u5f55 <sup>5<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">3.2 MED23\uff1a\u4fe1\u53f7\u6574\u5408\u4e0e RNA \u52a0\u5de5\u7684\u67a2\u7ebd<\/h3>\n\n\n\n<p><strong>MED23<\/strong>\uff08\u4ee5\u524d\u79f0\u4e3a Sur2\uff09\u662f\u8fde\u63a5 Ras\/MAPK \u4fe1\u53f7\u901a\u8def\u4e0e\u8f6c\u5f55\u673a\u5668\u7684\u5173\u952e\u8282\u70b9\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u4fe1\u53f7\u6574\u5408\uff1a<\/strong> \u5f53 MAPK \u901a\u8def\u88ab\u6fc0\u6d3b\u65f6\uff0c\u8f6c\u5f55\u56e0\u5b50 ELK1 \u88ab\u78f7\u9178\u5316\uff0c\u968f\u540e\u7279\u5f02\u6027\u7ed3\u5408 MED23\uff0c\u4ece\u800c\u62db\u52df Mediator \u6fc0\u6d3b\u65e9\u671f\u53cd\u5e94\u57fa\u56e0\uff08IEGs\uff09\u3002<\/li>\n\n\n\n<li><strong>RNA \u52a0\u5de5\u8fde\u63a5\uff1a<\/strong> \u8fd9\u662f MED23 \u6700\u72ec\u7279\u7684\u975e\u5178\u578b\u529f\u80fd\u3002\u7814\u7a76\u53d1\u73b0\uff0cMED23 \u80fd\u7269\u7406\u6027\u5730\u62db\u52df\u526a\u63a5\u56e0\u5b50 <strong>hnRNP L<\/strong> \u548c 3&#8242; \u7aef\u52a0\u5de5\u56e0\u5b50 <strong>CPSF<\/strong>\u3002\u8fd9\u610f\u5473\u7740 MED23 \u4e0d\u4ec5\u542f\u52a8\u8f6c\u5f55\uff0c\u8fd8\u534f\u8c03\u4e86\u968f\u540e\u7684 mRNA \u526a\u63a5\u548c\u52a0\u5c3e\u8fc7\u7a0b\uff0c\u662f\u201c\u8f6c\u5f55-\u52a0\u5de5\u5076\u8054\u201d\u7684\u5173\u952e\u5206\u5b50\u57fa\u7840 <sup>7<\/sup>\u3002\u8fd9\u4e00\u7279\u6027\u4e0e\u5176\u548c RTCB \u7684\u6f5c\u5728\u4e92\u4f5c\u5bc6\u5207\u76f8\u5173\uff08\u8be6\u89c1\u7b2c 5 \u8282\uff09\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">3.3 MED12 \u4e0e MED13\uff1a\u6fc0\u9176\u6a21\u5757\u7684\u7ed3\u6784\u57fa\u7840\u4e0e\u75be\u75c5\u70ed\u70b9<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>MED12\uff1a<\/strong> \u4f5c\u4e3a\u6fc0\u9176\u6a21\u5757\u7684\u652f\u67b6\uff0cMED12 \u5bf9\u4e8e\u6fc0\u6d3b CDK8 \u81f3\u5173\u91cd\u8981\u3002\u5728\u4e34\u5e8a\u4e0a\uff0cMED12 \u5916\u663e\u5b50 2 \u7684\u4f53\u7ec6\u80de\u7a81\u53d8\u5728 <strong>70% \u7684\u5b50\u5bab\u5e73\u6ed1\u808c\u7624\uff08\u5b50\u5bab\u808c\u7624\uff09<\/strong> \u4e2d\u88ab\u53d1\u73b0\uff0c\u540c\u65f6\u4e5f\u5e38\u89c1\u4e8e\u524d\u5217\u817a\u764c\u548c\u4e73\u817a\u7ea4\u7ef4\u817a\u7624\u3002\u8fd9\u4e9b\u7a81\u53d8\u901a\u5e38\u7834\u574f\u4e86\u6fc0\u9176\u6a21\u5757\u4e0e\u6838\u5fc3 Mediator \u7684\u901a\u8baf <sup>8<\/sup>\u3002<\/li>\n\n\n\n<li><strong>MED13\uff1a<\/strong> \u5b83\u662f\u6fc0\u9176\u6a21\u5757\u8fde\u63a5\u5230\u6838\u5fc3 Mediator \u7684\u7269\u7406\u201c\u6302\u94a9\u201d\u3002\u6709\u8da3\u7684\u662f\uff0cMED13 \u5728\u4ee3\u8c22\u8c03\u63a7\u4e2d\u626e\u6f14\u91cd\u8981\u89d2\u8272\u3002\u5728\u5fc3\u810f\u4e2d\u8fc7\u8868\u8fbe MED13 \u53ef\u4ee5\u4fc3\u8fdb\u5168\u8eab\u8102\u8d28\u6c27\u5316\uff0c\u62b5\u6297\u80a5\u80d6\u3002\u6b64\u5916\uff0cMED13 \u5728\u7ec6\u80de\u538b\u529b\u4e0b\u53ef\u4ece\u7ec6\u80de\u6838\u8f6c\u79fb\u5230\u7ec6\u80de\u8d28\uff0c\u53c2\u4e0e P-body\uff08RNA \u964d\u89e3\u5c0f\u4f53\uff09\u7684\u7ec4\u88c5\uff0c\u8fd9\u663e\u793a\u4e86 Mediator \u7ec4\u5206\u5728\u8f6c\u5f55\u4e4b\u5916\u7684\u201c\u517c\u804c\u201d\u529f\u80fd <sup>14<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">3.4 MED15\uff1a\u591a\u91cd\u836f\u7269\u6297\u6027\u4e0e\u8102\u8d28\u4ee3\u8c22<\/h3>\n\n\n\n<p><strong>MED15<\/strong> \u4f4d\u4e8e\u5c3e\u90e8\u6a21\u5757\uff0c\u5176 KIX \u7ed3\u6784\u57df\u5177\u6709\u6781\u5f3a\u7684\u7ed3\u5408\u591a\u80fd\u6027\u3002\u5b83\u80fd\u7ed3\u5408\u9175\u6bcd\u7684 Pdr1\uff08\u8c03\u63a7\u591a\u836f\u6297\u6027\u6cf5\uff09\u548c\u54fa\u4e73\u52a8\u7269\u7684 SREBP\uff08\u8c03\u63a7\u80c6\u56fa\u9187\u548c\u8102\u80aa\u9178\u5408\u6210\uff09\u3002\u56e0\u6b64\uff0cMED15 \u662f\u771f\u83cc\u836f\u7269\u6297\u6027\u548c\u4eba\u7c7b\u8102\u8d28\u4ee3\u8c22\u7d0a\u4e71\u7684\u5173\u952e\u8c03\u8282\u56e0\u5b50 <sup>6<\/sup>\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">4. \u7814\u7a76\u8fdb\u5c55\uff1a\u4ece\u67d3\u8272\u8d28\u73af\u5230\u76f8\u5206\u79bb<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">4.1 \u67d3\u8272\u8d28\u73af\u4e0e Cohesin \u7684\u534f\u540c\u4f5c\u7528<\/h3>\n\n\n\n<p>\u4f20\u7edf\u89c2\u70b9\u8ba4\u4e3a Mediator \u53ea\u662f\u586b\u8865\u4e86 Activator \u548c Pol II \u4e4b\u95f4\u7684\u7a7a\u95f4\u3002\u4f46\u6700\u65b0\u7684 <strong>Hi-C\uff08\u67d3\u8272\u8d28\u6784\u8c61\u6355\u83b7\uff09<\/strong> \u548c <strong>Micro-Capture-C<\/strong> \u6280\u672f\u63ed\u793a\uff0cMediator \u5b9e\u9645\u4e0a\u662f 3D \u57fa\u56e0\u7ec4\u67b6\u6784\u7684\u5efa\u7b51\u5e08\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u73af\u6324\u51fa\u7a33\u5b9a\u6a21\u578b\uff1a<\/strong> \u57fa\u56e0\u7ec4\u4e2d\u7684 Cohesin \u590d\u5408\u7269\uff08\u9ecf\u8fde\u86cb\u767d\uff09\u901a\u8fc7\u201c\u73af\u6324\u51fa\uff08Loop Extrusion\uff09\u201d\u673a\u5236\u5f62\u6210\u67d3\u8272\u8d28\u73af\u3002\u5f53 Cohesin \u9047\u5230\u7ed3\u5408\u5728\u589e\u5f3a\u5b50\u548c\u542f\u52a8\u5b50\u4e0a\u7684 Mediator \u590d\u5408\u7269\u65f6\uff0c\u73af\u6324\u51fa\u505c\u6b62\u6216\u88ab\u7a33\u5b9a\u4e0b\u6765\u3002<\/li>\n\n\n\n<li><strong>\u8bc1\u636e\uff1a<\/strong> \u5feb\u901f\u964d\u89e3 MED14\uff08\u5bfc\u81f4 Mediator \u6838\u5fc3\u89e3\u4f53\uff09\u4f1a\u76f4\u63a5\u5bfc\u81f4\u7279\u5b9a\u589e\u5f3a\u5b50-\u542f\u52a8\u5b50\u73af\uff08E-P Loops\uff09\u7684\u6d88\u5931\uff0c\u4ee5\u53ca Cohesin \u5728\u57fa\u56e0\u7ec4\u4e0a\u7684\u91cd\u65b0\u5206\u5e03\u3002\u8fd9\u8bc1\u660e\u4e86 Mediator \u548c Cohesin \u7269\u7406\u4e0a\u548c\u529f\u80fd\u4e0a\u534f\u540c\u5de5\u4f5c\uff0c\u5171\u540c\u62c9\u8fd1\u8fdc\u7aef\u8c03\u63a7\u5143\u4ef6 <sup>4<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">4.2 \u8d85\u7ea7\u589e\u5f3a\u5b50\u4e0e\u8f6c\u5f55\u51dd\u805a\u4f53<\/h3>\n\n\n\n<p>\u5982\u524d\u6240\u8ff0\uff0c\u76f8\u5206\u79bb\u7406\u8bba\u662f\u76ee\u524d Mediator \u7814\u7a76\u7684\u6700\u524d\u6cbf\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u8d85\u7ea7\u589e\u5f3a\u5b50\uff08SEs\uff09\uff1a<\/strong> \u662f\u4e00\u5927\u7c07\u5bc6\u96c6\u7684\u589e\u5f3a\u5b50\u533a\u57df\uff0c\u7ed3\u5408\u4e86\u6781\u9ad8\u5bc6\u5ea6\u7684 Mediator\u3001BRD4 \u548c\u4e3b\u63a7\u8f6c\u5f55\u56e0\u5b50\u3002SEs \u9a71\u52a8\u4e86\u51b3\u5b9a\u7ec6\u80de\u8eab\u4efd\uff08Cell Identity\uff09\u7684\u5173\u952e\u57fa\u56e0\u8868\u8fbe\u3002<\/li>\n\n\n\n<li><strong>\u836f\u7269\u9776\u70b9\uff1a<\/strong> \u764c\u7ec6\u80de\uff08\u5982\u591a\u53d1\u6027\u9aa8\u9ad3\u7624\u7ec6\u80de\u4e2d\u7684 <em>MYC<\/em> \u57fa\u56e0\uff09\u5f80\u5f80\u4f9d\u8d56\u8d85\u7ea7\u589e\u5f3a\u5b50\u7ef4\u6301\u5176\u6076\u6027\u8868\u578b\u3002\u7834\u574f Mediator \u7ed3\u6784\u7684\u5b8c\u6574\u6027\u6216\u4f7f\u7528 BRD4 \u6291\u5236\u5242\uff08\u5982 JQ1\uff09\u53ef\u4ee5\u7279\u5f02\u6027\u74e6\u89e3\u8fd9\u4e9b\u8d85\u7ea7\u589e\u5f3a\u5b50\u51dd\u805a\u4f53\uff0c\u4ece\u800c\u9009\u62e9\u6027\u6740\u6b7b\u764c\u7ec6\u80de\u3002\u8fd9\u4f7f\u5f97 Mediator \u53ca\u5176\u4e92\u4f5c\u86cb\u767d\u6210\u4e3a\u70ed\u95e8\u7684\u6297\u764c\u836f\u7269\u9776\u70b9 <sup>9<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">5. Mediator \u4e0e RNA Ligation (\u8fde\u63a5) \u53ca RTCB \u7684\u6df1\u5ea6\u5173\u8054<\/h2>\n\n\n\n<p>\u7528\u6237\u7279\u522b\u8be2\u95ee\u4e86 <strong>Mediator \u4e0e RNA ligation \u4ee5\u53ca RTCB \u662f\u5426\u5b58\u5728\u5173\u8054<\/strong>\u3002\u8fd9\u662f\u4e00\u4e2a\u975e\u5e38\u4e13\u4e1a\u4e14\u524d\u6cbf\u7684\u95ee\u9898\u3002\u867d\u7136\u6559\u79d1\u4e66\u5e38\u5c06 Mediator\uff08\u8f6c\u5f55\uff09\u4e0e RNA \u8fde\u63a5\u9176\uff08RNA \u52a0\u5de5\uff09\u89c6\u4e3a\u4e24\u4e2a\u72ec\u7acb\u7684\u7cfb\u7edf\uff0c\u4f46\u6df1\u5165\u7684\u6587\u732e\u6316\u6398\u63ed\u793a\u4e86\u5b83\u4eec\u5728 <strong>\u7ec6\u80de\u538b\u529b\u54cd\u5e94\uff08UPR\uff09<\/strong>\u3001<strong>\u7269\u7406\u4e92\u4f5c<\/strong> \u4ee5\u53ca <strong>\u7a7a\u95f4\u5b9a\u4f4d<\/strong> \u4e0a\u7684\u7d27\u5bc6\u4ea4\u7ec7\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">5.1 \u4ec0\u4e48\u662f RTCB \u4e0e tRNA \u8fde\u63a5\u9176\u590d\u5408\u7269\uff1f<\/h3>\n\n\n\n<p>\u9996\u5148\u9700\u8981\u5b9a\u4e49 <strong>RTCB<\/strong> (RNA 2&#8242;,3&#8242;-cyclic phosphate and 5&#8242;-OH ligase)\u3002\u5b83\u662f\u540e\u751f\u52a8\u7269 <strong>tRNA \u8fde\u63a5\u9176\u590d\u5408\u7269 (tRNA-LC)<\/strong> \u7684\u6838\u5fc3\u50ac\u5316\u4e9a\u57fa\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u72ec\u7279\u6d3b\u6027\uff1a<\/strong> \u4e0e\u4f20\u7edf\u7684 ATP \u4f9d\u8d56\u6027\u8fde\u63a5\u9176\uff08\u8fde\u63a5 5&#8242;-PO4 \u548c 3&#8242;-OH\uff09\u4e0d\u540c\uff0cRTCB \u662f\u4e00\u79cd GTP \u4f9d\u8d56\u6027\u8fde\u63a5\u9176\uff0c\u4e13\u95e8\u8fde\u63a5\u5177\u6709 <strong>2&#8242;,3&#8242;-\u73af\u78f7\u9178<\/strong> \u548c <strong>5&#8242;-\u7f9f\u57fa<\/strong> \u672b\u7aef\u7684 RNA \u7247\u6bb5 <sup>19<\/sup>\u3002<\/li>\n\n\n\n<li><strong>\u590d\u5408\u7269\u7ec4\u5206\uff1a<\/strong> \u5728\u4eba\u4f53\u5185\uff0cRTCB \u5e76\u975e\u5355\u72ec\u884c\u52a8\uff0c\u800c\u662f\u4e0e <strong>DDX1<\/strong>\uff08\u89e3\u65cb\u9176\uff09\u3001<strong>RTRAF<\/strong> (CGI-99\/hCLE)\u3001<strong>FAM98B<\/strong> \u548c <strong>Ashwin<\/strong> \u7ec4\u6210\u4e94\u805a\u4f53\u590d\u5408\u7269 <sup>19<\/sup>\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">5.2 \u6838\u5fc3\u5173\u8054\u4e00\uff1a\u672a\u6298\u53e0\u86cb\u767d\u53cd\u5e94 (UPR) \u4e2d\u7684\u529f\u80fd\u7ea7\u8054<\/h3>\n\n\n\n<p>Mediator \u4e0e RTCB \u6700\u786e\u51ff\u7684\u751f\u7269\u5b66\u8054\u7cfb\u5728\u4e8e <strong>IRE1\u03b1-XBP1 \u901a\u8def<\/strong>\uff0c\u8fd9\u662f\u7ec6\u80de\u5e94\u5bf9\u5185\u8d28\u7f51\u538b\u529b\uff08ER Stress\uff09\u7684\u6838\u5fc3\u673a\u5236\u3002<\/p>\n\n\n\n<ol start=\"1\" class=\"wp-block-list\">\n<li><strong>\u4e0a\u6e38\u526a\u63a5\u4e0e\u8fde\u63a5 (RTCB \u7684\u89d2\u8272)\uff1a<\/strong> \u5f53\u7ec6\u80de\u611f\u77e5\u5230\u5185\u8d28\u7f51\u538b\u529b\u65f6\uff0cIRE1\u03b1 \u88ab\u6fc0\u6d3b\u5e76\u5728\u7ec6\u80de\u8d28\u4e2d\u5207\u5272 <em>XBP1u<\/em> mRNA\uff0c\u5207\u9664\u4e00\u4e2a 26nt \u7684\u5185\u542b\u5b50\u3002\u7559\u4e0b\u7684\u65ad\u7aef\u6b63\u662f 2&#8242;,3&#8242;-\u73af\u78f7\u9178\u548c 5&#8242;-OH\u3002<strong>RTCB<\/strong> \u662f\u552f\u4e00\u80fd\u591f\u8fde\u63a5\u8fd9\u4e24\u4e2a\u65ad\u7aef\u751f\u6210\u6210\u719f <em>XBP1s<\/em> mRNA \u7684\u8fde\u63a5\u9176 <sup>20<\/sup>\u3002<\/li>\n\n\n\n<li><strong>\u8f6c\u5f55\u56e0\u5b50\u751f\u6210\uff1a<\/strong> \u53ea\u6709\u7ecf\u8fc7 RTCB \u8fde\u63a5\u7684 mRNA \u624d\u80fd\u7ffb\u8bd1\u51fa\u6709\u529f\u80fd\u7684\u8f6c\u5f55\u56e0\u5b50 <strong>XBP1s<\/strong>\u3002<\/li>\n\n\n\n<li><strong>\u4e0b\u6e38\u8f6c\u5f55\u6fc0\u6d3b (Mediator \u7684\u89d2\u8272)\uff1a<\/strong> XBP1s \u8fdb\u5165\u7ec6\u80de\u6838\u540e\uff0c\u5fc5\u987b\u62db\u52df <strong>Mediator \u590d\u5408\u7269<\/strong> \u624d\u80fd\u6fc0\u6d3b\u5176\u9776\u57fa\u56e0\uff08\u5982\u4f34\u4fa3\u86cb\u767d BiP\u3001ERAD \u7ec4\u5206\uff09\u3002XBP1s \u5c5e\u4e8e bZIP \u5bb6\u65cf\u8f6c\u5f55\u56e0\u5b50\uff0c\u8fd9\u7c7b\u56e0\u5b50\u901a\u5e38\u901a\u8fc7 <strong>Tail \u6a21\u5757\uff08\u7279\u522b\u662f MED15\uff09<\/strong> \u62db\u52df Mediator <sup>23<\/sup>\u3002<\/li>\n\n\n\n<li><strong>\u7ed3\u8bba\uff1a<\/strong> RTCB \u548c Mediator \u662f\u540c\u4e00\u6761\u751f\u5b58\u901a\u8def\uff08UPR\uff09\u4e2d\u7684\u4e0a\u4e0b\u6e38\u5173\u952e\u8282\u70b9\u3002RTCB \u8d1f\u8d23\u201c\u5236\u9020\u201d\u8f6c\u5f55\u56e0\u5b50\uff0c\u800c Mediator \u8d1f\u8d23\u201c\u6267\u884c\u201d\u8be5\u8f6c\u5f55\u56e0\u5b50\u7684\u6307\u4ee4\u3002RTCB \u7684\u7f3a\u5931\u4f1a\u963b\u65ad XBP1s \u7684\u4ea7\u751f\uff0c\u4ece\u800c\u5bfc\u81f4 Mediator \u65e0\u6cd5\u88ab\u62db\u52df\u5230 UPR \u9776\u57fa\u56e0\u4e0a\uff0c\u6700\u7ec8\u5bfc\u81f4\u7ec6\u80de\u65e0\u6cd5\u5e94\u5bf9\u538b\u529b\u800c\u51cb\u4ea1 <sup>22<\/sup>\u3002<\/li>\n<\/ol>\n\n\n\n<h3 class=\"wp-block-heading\">5.3 \u6838\u5fc3\u5173\u8054\u4e8c\uff1a\u7269\u7406\u4e92\u4f5c\u4e0e\u86cb\u767d\u8d28\u7ec4\u5b66\u8bc1\u636e<\/h3>\n\n\n\n<p>\u9664\u4e86\u529f\u80fd\u4e0a\u7684\u4e0a\u4e0b\u6e38\u5173\u7cfb\uff0c\u662f\u5426\u5b58\u5728\u76f4\u63a5\u7684\u7269\u7406\u7ed3\u5408\uff1f<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u9ad8\u901a\u91cf\u7b5b\u9009\u8bc1\u636e\uff1a<\/strong> \u5728 HitPredict \u7b49\u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u6570\u636e\u5e93\u4e2d\uff0c\u8bb0\u5f55\u4e86 <strong>RTCB \u4e0e Mediator \u4e9a\u57fa\uff08\u5c24\u5176\u662f MED23 \u548c MED15\uff09<\/strong> \u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528 <sup>27<\/sup>\u3002<\/li>\n\n\n\n<li><strong>MED23 \u7684\u201c\u505c\u6cca\u6e2f\u201d\u5047\u8bf4\uff1a<\/strong> \u5982\u524d\u6240\u8ff0\uff083.2\u8282\uff09\uff0cMED23 \u5df2\u77e5\u80fd\u7ed3\u5408\u526a\u63a5\u56e0\u5b50 hnRNP L \u548c 3&#8242; \u7aef\u52a0\u5de5\u56e0\u5b50 CPSF <sup>7<\/sup>\u3002\u53d1\u73b0 MED23 \u4e0e RTCB \u5b58\u5728\u4e92\u4f5c\u6697\u793a\u4e86\u4e00\u4e2a\u6781\u5176\u91cd\u8981\u7684\u673a\u5236\uff1aMediator \u53ef\u80fd\u4f5c\u4e3a\u4e00\u4e2a\u5e73\u53f0\uff0c\u5c06 RNA \u8fde\u63a5\u9176\uff08RTCB\uff09\u76f4\u63a5\u62db\u52df\u5230\u8f6c\u5f55\u6d3b\u8dc3\u7684\u57fa\u56e0\u9644\u8fd1\u3002<\/li>\n\n\n\n<li><strong>\u751f\u7269\u5b66\u610f\u4e49\uff1a<\/strong> \u8fd9\u79cd\u62db\u52df\u53ef\u80fd\u4f7f\u5f97 <em>XBP1<\/em> mRNA \u5728\u8f6c\u5f55\u540e\u80fd\u88ab\u8fc5\u901f\u52a0\u5de5\uff0c\u6216\u8005\u5728 tRNA \u8f6c\u5f55\uff08\u7531 Pol III \u8d1f\u8d23\uff0c\u4f46 Pol II \u56e0\u5b50\u5e38\u6709\u534f\u52a9\u4f5c\u7528\uff09\u533a\u57df\u63d0\u4f9b\u4fee\u590d\u652f\u6301\u3002\u8003\u8651\u5230 DDX1\uff08tRNA-LC \u7ec4\u5206\uff09\u4e5f\u53c2\u4e0e DNA \u4fee\u590d\uff0c\u8fd9\u79cd Mediator-RTCB \u7684\u7269\u7406\u8fde\u63a5\u53ef\u80fd\u4e5f\u6d89\u53ca\u8f6c\u5f55\u5076\u8054\u7684 DNA\/RNA \u635f\u4f24\u4fee\u590d\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">5.4 \u6838\u5fc3\u5173\u8054\u4e09\uff1aRTRAF (hCLE) \u7684\u8f6c\u5f55\u5076\u8054\u529f\u80fd<\/h3>\n\n\n\n<p>tRNA \u8fde\u63a5\u9176\u590d\u5408\u7269\u4e2d\u7684\u53e6\u4e00\u4e2a\u4e9a\u57fa <strong>RTRAF (hCLE)<\/strong> \u63d0\u4f9b\u4e86\u66f4\u76f4\u63a5\u7684\u8f6c\u5f55\u8054\u7cfb\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u4e0e Pol II \u7684\u5171\u5b9a\u4f4d\uff1a<\/strong> RTRAF \u4e0d\u4ec5\u662f RTCB \u7684\u7ed3\u5408\u4f19\u4f34\uff0c\u7814\u7a76\u8fd8\u53d1\u73b0\u5b83\u4e0e\u6d3b\u6027 RNA \u805a\u5408\u9176 II (Ser5-P Pol II) \u5728\u6838\u5185\u5171\u5b9a\u4f4d\u3002\u6572\u4f4e RTRAF \u4f1a\u5bfc\u81f4\u5168\u7ec6\u80de RNA \u8f6c\u5f55\u6c34\u5e73\u4e0b\u964d 50% <sup>28<\/sup>\u3002<\/li>\n\n\n\n<li><strong>\u7a7f\u68ad\u673a\u5236\uff1a<\/strong> RTRAF \u88ab\u8ba4\u4e3a\u662f tRNA-LC \u590d\u5408\u7269\u5728\u7ec6\u80de\u6838\u4e0e\u7ec6\u80de\u8d28\u4e4b\u95f4\u7a7f\u68ad\u7684\u201c\u5411\u5bfc\u201d\u3002\u5b83\u4e0e Pol II \u7684\u7d27\u5bc6\u8054\u7cfb\u6697\u793a\uff0cRTCB \u590d\u5408\u7269\u53ef\u80fd\u5728\u8f6c\u5f55\u4f4d\u70b9\u9644\u8fd1\u5c31\u88ab\u7ec4\u88c5\u6216\u88c5\u8f7d\uff0c\u968f\u8f6c\u5f55\u4ea7\u7269\u4e00\u540c\u8f93\u51fa\u6216\u5728\u6838\u5185\u76f4\u63a5\u53d1\u6325\u975e\u5178\u578b\u4f5c\u7528\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">5.5 \u5173\u8054\u603b\u7ed3\uff1a\u4ece\u8f6c\u5f55\u5230 RNA \u4fee\u590d\u7684\u6574\u5408<\/h3>\n\n\n\n<p>\u7efc\u4e0a\u6240\u8ff0\uff0cMediator \u4e0e RTCB \u7684\u5173\u8054\u5e76\u975e\u7a7a\u7a74\u6765\u98ce\uff0c\u800c\u662f\u591a\u5c42\u6b21\u7684\uff1a<\/p>\n\n\n\n<ol start=\"1\" class=\"wp-block-list\">\n<li><strong>\u529f\u80fd\u5c42\u9762\uff1a<\/strong> \u5728 UPR \u901a\u8def\u4e2d\uff0cRTCB \u662f Mediator \u9a71\u52a8\u7684\u57fa\u56e0\u8868\u8fbe\u7a0b\u5e8f\u7684\u5fc5\u8981\u4e0a\u6e38\u5f00\u5173\u3002<\/li>\n\n\n\n<li><strong>\u7269\u7406\u5c42\u9762\uff1a<\/strong> \u5b58\u5728 MED23\/MED15 \u4e0e RTCB \u4e92\u4f5c\u7684\u86cb\u767d\u8d28\u7ec4\u5b66\u8bc1\u636e\uff0c\u652f\u6301 Mediator \u62db\u52df RNA \u52a0\u5de5\u673a\u5668\u7684\u6a21\u578b\u3002<\/li>\n\n\n\n<li><strong>\u7a7a\u95f4\u5c42\u9762\uff1a<\/strong> RTCB \u7684\u4f34\u4fa3\u86cb\u767d RTRAF \u76f4\u63a5\u4e0e\u8f6c\u5f55\u673a\u5668\uff08Pol II\uff09\u5171\u5b9a\u4f4d\uff0c\u6697\u793a\u4e86\u8f6c\u5f55\u4e0e RNA \u8fde\u63a5\u7684\u7a7a\u95f4\u5076\u8054\u3002<\/li>\n<\/ol>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\">6. \u603b\u7ed3\u4e0e\u5c55\u671b<\/h2>\n\n\n\n<p>Mediator \u590d\u5408\u7269\u4f5c\u4e3a\u771f\u6838\u751f\u7269\u57fa\u56e0\u8f6c\u5f55\u7684\u201c\u4e2d\u67a2\u795e\u7ecf\u7cfb\u7edf\u201d\uff0c\u5176\u529f\u80fd\u65e9\u5df2\u8d85\u8d8a\u4e86\u5355\u7eaf\u7684\u8f6c\u5f55\u5171\u6fc0\u6d3b\u3002\u901a\u8fc7\u5bf9 <strong>MED1<\/strong> \u9a71\u52a8\u7684\u76f8\u5206\u79bb\u3001<strong>MED23<\/strong> \u4ecb\u5bfc\u7684 RNA \u52a0\u5de5\u5076\u8054\u3001\u4ee5\u53ca <strong>\u6fc0\u9176\u6a21\u5757<\/strong> \u7684\u4ee3\u8c22\u8c03\u63a7\u7684\u7814\u7a76\uff0c\u6211\u4eec\u6b63\u5728\u6784\u5efa\u4e00\u5e45\u5168\u666f\u5f0f\u7684\u57fa\u56e0\u8c03\u63a7\u56fe\u8c31\u3002<\/p>\n\n\n\n<p>\u5bf9\u4e8e\u7528\u6237\u5173\u5fc3\u7684 <strong>RTCB \u5173\u8054<\/strong>\uff0c\u73b0\u6709\u8bc1\u636e\u63cf\u7ed8\u4e86\u4e00\u4e2a\u7cbe\u5999\u7684\u7ec6\u80de\u538b\u529b\u54cd\u5e94\u7f51\u7edc\uff1a\u7ec6\u80de\u5229\u7528 RTCB \u5728\u7ec6\u80de\u8d28\uff08\u6216\u6838\u5185\uff09\u7684\u8fde\u63a5\u6d3b\u6027\u6765\u611f\u77e5\u538b\u529b\uff0c\u5e76\u901a\u8fc7\u751f\u6210 XBP1s \u5c06\u4fe1\u53f7\u4f20\u56de\u7ec6\u80de\u6838\uff1b\u800c\u5728\u6838\u5185\uff0cMediator \u590d\u5408\u7269\u4e0d\u4ec5\u4f5c\u4e3a XBP1s \u7684\u6267\u884c\u8005\u542f\u52a8\u8f6c\u5f55\uff0c\u8fd8\u53ef\u80fd\u901a\u8fc7 MED23 \u7b49\u4e9a\u57fa\u76f4\u63a5\u4e0e RTCB \u590d\u5408\u7269\u4fdd\u6301\u7269\u7406\u63a5\u89e6\uff0c\u4ee5\u786e\u4fdd\u8f6c\u5f55\u4e0e RNA \u52a0\u5de5\u3001\u4fee\u590d\u7684\u9ad8\u6548\u534f\u540c\u3002\u672a\u6765\u7684\u7814\u7a76\u53ef\u80fd\u4f1a\u8fdb\u4e00\u6b65\u63ed\u793a\u8fd9\u79cd\u201c\u8f6c\u5f55-\u8fde\u63a5\u9176\u201d\u590d\u5408\u7269\u5728\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\uff08\u5982 RTCB \u7a81\u53d8\u5bfc\u81f4\u7684\u8111\u6865\u5c0f\u8111\u53d1\u80b2\u4e0d\u5168\uff09\u548c\u764c\u75c7\u4e2d\u7684\u75c5\u7406\u673a\u5236\u3002<\/p>\n\n\n\n<p><strong>\u6838\u5fc3\u7ed3\u8bba\u8868\uff1aMediator \u4e0e RNA \u8fde\u63a5\u9176\u7cfb\u7edf\u7684\u4ea4\u4e92<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><td><strong>\u4ea4\u4e92\u5c42\u9762<\/strong><\/td><td><strong>\u6d89\u53ca Mediator \u7ec4\u5206<\/strong><\/td><td><strong>\u6d89\u53ca RNA \u8fde\u63a5\u7ec4\u5206<\/strong><\/td><td><strong>\u673a\u5236\u63cf\u8ff0<\/strong><\/td><\/tr><\/thead><tbody><tr><td><strong>\u4fe1\u53f7\u901a\u8def (UPR)<\/strong><\/td><td>Tail \u6a21\u5757 (MED15)<\/td><td>RTCB, IRE1\u03b1<\/td><td>RTCB \u8fde\u63a5 <em>XBP1<\/em> mRNA -&gt; \u751f\u6210 XBP1s -&gt; \u62db\u52df Mediator -&gt; \u6fc0\u6d3b\u4f34\u4fa3\u86cb\u767d\u57fa\u56e0\u3002<\/td><\/tr><tr><td><strong>\u7269\u7406\u4e92\u4f5c (Proteomics)<\/strong><\/td><td>MED23, MED15<\/td><td>RTCB<\/td><td>\u9ad8\u901a\u91cf\u7b5b\u9009\u663e\u793a\u7269\u7406\u7ed3\u5408\uff1bMED23 \u53ef\u80fd\u4f5c\u4e3a RNA \u52a0\u5de5\u673a\u5668\u7684\u505c\u6cca\u5e73\u53f0\u3002<\/td><\/tr><tr><td><strong>\u8f6c\u5f55\u5076\u8054 (Coupling)<\/strong><\/td><td>Pol II \u673a\u5668<\/td><td>RTRAF (hCLE)<\/td><td>RTCB \u7684\u4f34\u4fa3 RTRAF \u4e0e Pol II \u5171\u5b9a\u4f4d\u5e76\u4fc3\u8fdb\u8f6c\u5f55\uff1b\u6697\u793a\u8fde\u63a5\u9176\u590d\u5408\u7269\u5728\u8f6c\u5f55\u4f4d\u70b9\u7684\u5bcc\u96c6\u3002<\/td><\/tr><tr><td><strong>\u5e94\u6fc0\u7a7f\u68ad (Shuttling)<\/strong><\/td><td>MED13, Cyclin C<\/td><td>RTCB \u590d\u5408\u7269<\/td><td>\u4e24\u8005\u5747\u5177\u5907\u5728\u538b\u529b\u4e0b\u6838-\u8d28\u7a7f\u68ad\u7684\u80fd\u529b\uff0c\u534f\u8c03\u7ec6\u80de\u6838\u8f6c\u5f55\u4e0e\u7ec6\u80de\u8d28 RNA \u4ee3\u8c22\/\u964d\u89e3 (P-bodies)\u3002<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>\u901a\u8fc7\u7406\u89e3\u8fd9\u4e9b\u590d\u6742\u7684\u5206\u5b50\u8054\u7cfb\uff0c\u6211\u4eec\u4e0d\u4ec5\u80fd\u66f4\u6df1\u523b\u5730\u8ba4\u8bc6\u57fa\u56e0\u8868\u8fbe\u7684\u57fa\u7840\u751f\u7269\u5b66\uff0c\u4e5f\u80fd\u4e3a\u5f00\u53d1\u9488\u5bf9\u764c\u75c7\uff08\u9776\u5411\u8d85\u7ea7\u589e\u5f3a\u5b50\uff09\u548c\u4ee3\u8c22\u75be\u75c5\uff08\u9776\u5411 CDK8 \u6fc0\u9176\uff09\u7684\u65b0\u7597\u6cd5\u63d0\u4f9b\u7406\u8bba\u652f\u6491\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><strong>\u5f15\u7528\u7684\u8457\u4f5c<\/strong><\/h4>\n\n\n\n<ol class=\"wp-block-list\">\n<li>The Mediator complex: a master coordinator of transcription and cell lineage development, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/journals.biologists.com\/dev\/article\/141\/5\/977\/46589\/The-Mediator-complex-a-master-coordinator-of\">https:\/\/journals.biologists.com\/dev\/article\/141\/5\/977\/46589\/The-Mediator-complex-a-master-coordinator-of<\/a><\/li>\n\n\n\n<li>The complex structure and function of Mediator &#8211; PMC &#8211; PubMed Central, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6130968\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6130968\/<\/a><\/li>\n\n\n\n<li>Mediator binds to boundaries of chromosomal interaction domains and to proteins involved in DNA looping, RNA metabolism &#8211; Oxford Academic, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/academic.oup.com\/nar\/article-pdf\/45\/15\/8806\/19697353\/gkx491.pdf\">https:\/\/academic.oup.com\/nar\/article-pdf\/45\/15\/8806\/19697353\/gkx491.pdf<\/a><\/li>\n\n\n\n<li>(PDF) The Mediator complex regulates enhancer-promoter interactions &#8211; ResearchGate, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.researchgate.net\/publication\/372248928_The_Mediator_complex_regulates_enhancer-promoter_interactions\">https:\/\/www.researchgate.net\/publication\/372248928_The_Mediator_complex_regulates_enhancer-promoter_interactions<\/a><\/li>\n\n\n\n<li>Coactivator condensation at super-enhancers links phase separation and gene control &#8211; NIH, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6092193\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6092193\/<\/a><\/li>\n\n\n\n<li>The metazoan Mediator co-activator complex as an integrative hub for transcriptional regulation | Request PDF &#8211; ResearchGate, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.researchgate.net\/publication\/47404019_The_metazoan_Mediator_co-activator_complex_as_an_integrative_hub_for_transcriptional_regulation\">https:\/\/www.researchgate.net\/publication\/47404019_The_metazoan_Mediator_co-activator_complex_as_an_integrative_hub_for_transcriptional_regulation<\/a><\/li>\n\n\n\n<li>Mediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit &#8211; PMC, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3288850\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3288850\/<\/a><\/li>\n\n\n\n<li>Regulatory functions of the Mediator kinases CDK8 and CDK19 &#8211; PMC &#8211; PubMed Central, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6602567\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6602567\/<\/a><\/li>\n\n\n\n<li>Mediator condensates localize signaling factors to key cell identity genes &#8211; PMC, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6898777\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6898777\/<\/a><\/li>\n\n\n\n<li>Super-Enhancers, Phase-Separated Condensates, and 3D Genome &#8230;, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC9221043\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC9221043\/<\/a><\/li>\n\n\n\n<li>The Mediator subunit MED23 couples H2B mono\u2010ubiquitination to transcriptional control and cell fate determination &#8211; EMBO Press journals, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.embopress.org\/doi\/10.15252\/embj.201591279\">https:\/\/www.embopress.org\/doi\/10.15252\/embj.201591279<\/a><\/li>\n\n\n\n<li>Mediator regulates transcriptional termination through cross-talk with pre-mRNA 3&#8242; end processing factors | Request PDF &#8211; ResearchGate, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.researchgate.net\/publication\/392167088_Mediator_regulates_transcriptional_termination_through_cross-talk_with_pre-mRNA_3'_end_processing_factors\">https:\/\/www.researchgate.net\/publication\/392167088_Mediator_regulates_transcriptional_termination_through_cross-talk_with_pre-mRNA_3&#8217;_end_processing_factors<\/a><\/li>\n\n\n\n<li>MED12 Gene &#8211; Ma&#8217;ayan Lab \u2013 Computational Systems Biology, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/maayanlab.cloud\/Harmonizome\/gene\/MED12\">https:\/\/maayanlab.cloud\/Harmonizome\/gene\/MED12<\/a><\/li>\n\n\n\n<li>MED13 Gene &#8211; Ma&#8217;ayan Lab \u2013 Computational Systems Biology, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/maayanlab.cloud\/Harmonizome\/gene\/MED13\">https:\/\/maayanlab.cloud\/Harmonizome\/gene\/MED13<\/a><\/li>\n\n\n\n<li>Autophagy, P-Body Assembly, Mitochondrial Hyper-Fission, and Cell Death: Unexpected Secondary Roles of the Mediator Kinase Module Following Stress &#8211; Preprints.org, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.preprints.org\/manuscript\/202503.0744\/v1\">https:\/\/www.preprints.org\/manuscript\/202503.0744\/v1<\/a><\/li>\n\n\n\n<li>Mediator and Cohesin Connect Gene Expression and Chromatin &#8230;, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC2953795\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC2953795\/<\/a><\/li>\n\n\n\n<li>The Mediator complex regulates enhancer-promoter interactions &#8211; bioRxiv, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2022.06.15.496245v1.full.pdf\">https:\/\/www.biorxiv.org\/content\/10.1101\/2022.06.15.496245v1.full.pdf<\/a><\/li>\n\n\n\n<li>Super-enhancers in tumors: unraveling recent advances in their role in Oncogenesis and the emergence of targeted therapies &#8211; PMC &#8211; PubMed Central, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC11753147\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC11753147\/<\/a><\/li>\n\n\n\n<li>Molecular architecture of the human tRNA ligase complex &#8211; PMC &#8211; NIH, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC8668186\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC8668186\/<\/a><\/li>\n\n\n\n<li>Multiscale In Silico Study of the Mechanism of Activation of the RtcB Ligase by the PTP1B Phosphatase | Journal of Chemical Information and Modeling &#8211; ACS Publications, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.jcim.3c01600\">https:\/\/pubs.acs.org\/doi\/10.1021\/acs.jcim.3c01600<\/a><\/li>\n\n\n\n<li>Importance of Mediator complex in the regulation and integration of diverse signaling pathways in plants &#8211; Frontiers, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.frontiersin.org\/journals\/plant-science\/articles\/10.3389\/fpls.2015.00757\/full\">https:\/\/www.frontiersin.org\/journals\/plant-science\/articles\/10.3389\/fpls.2015.00757\/full<\/a><\/li>\n\n\n\n<li>The mammalian tRNA ligase complex mediates splicing of XBP1 mRNA and controls antibody secretion in plasma cells &#8211; PubMed Central, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC4282640\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC4282640\/<\/a><\/li>\n\n\n\n<li>Hac1 &#8211; Saccharomyces Genome Database | SGD, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.yeastgenome.org\/locus\/S000001863\">https:\/\/www.yeastgenome.org\/locus\/S000001863<\/a><\/li>\n\n\n\n<li>Emerging Roles for XBP1, a sUPeR Transcription Factor &#8211; PMC &#8211; PubMed Central, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3374844\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC3374844\/<\/a><\/li>\n\n\n\n<li>Roles of XBP1s in Transcriptional Regulation of Target Genes &#8211; PMC &#8211; PubMed Central, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC8301375\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC8301375\/<\/a><\/li>\n\n\n\n<li>The mammalian tRNA ligase complex mediates splicing of XBP1 mRNA and controls antibody secretion in plasma cells &#8211; EMBO Press journals, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.embopress.org\/doi\/10.15252\/embj.201490332\">https:\/\/www.embopress.org\/doi\/10.15252\/embj.201490332<\/a><\/li>\n\n\n\n<li>ESR1 &#8211; HitPredict &#8211; High confidence protein-protein interactions, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.hitpredict.org\/htp_int.php?Value=19384\">https:\/\/www.hitpredict.org\/htp_int.php?Value=19384<\/a><\/li>\n\n\n\n<li>Insights into the structure and function of the RNA ligase RtcB &#8211; PMC, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC10630183\/\">https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC10630183\/<\/a><\/li>\n<\/ol>\n\n\n\n<p>Role of C14orf166 in viral infection and RNA metabolism and its relationship with cancer, \u8bbf\u95ee\u65f6\u95f4\u4e3a \u5341\u4e8c\u6708 13, 2025\uff0c <a href=\"https:\/\/www.spandidos-publications.com\/10.3892\/mmr.2021.12039\">https:\/\/www.spandidos-publications.com\/10.3892\/mmr.2021.12039<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd9\u7bc7\u6df1\u5ea6\u7814\u7a76\u62a5\u544a\u7cfb\u7edf\u5256\u6790\u4e86\u771f\u6838\u8f6c\u5f55\u8c03\u63a7\u7684\u6838\u5fc3\u67a2\u7ebd\u2014\u2014Mediator\u590d\u5408\u7269\u3002\u6587\u7ae0\u4e0d\u4ec5\u8be6\u8ff0\u4e86\u5176\u6a21\u5757\u5316\u7ed3\u6784\u3001\u5173\u952e\u4e9a\u57fa\uff08\u5982MED1\u3001MED23\uff09\u7684\u529f\u80fd\u4e0e\u76f8\u5206\u79bb\u673a\u5236\uff0c\u66f4\u6df1\u5165\u63a2\u8ba8\u4e86\u5176\u4e0e\u67d3\u8272\u8d28\u67b6\u6784\u53caRNA\u8fde\u63a5\u9176RTCB\u5728\u672a\u6298\u53e0\u86cb\u767d\u53cd\u5e94\u7b49\u901a\u8def\u4e2d\u7684\u591a\u5c42\u6b21\u4ea4\u4e92\uff0c\u63ed\u793a\u4e86\u8f6c\u5f55\u4e0eRNA\u52a0\u5de5\u95f4\u7684\u7cbe\u5999\u5076\u8054\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[32],"class_list":["post-13622","post","type-post","status-publish","format-standard","hentry","category-18","tag-deep-research"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/13622","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13622"}],"version-history":[{"count":6,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/13622\/revisions"}],"predecessor-version":[{"id":13735,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/13622\/revisions\/13735"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13622"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13622"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13622"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}