{"id":14234,"date":"2025-12-21T13:45:17","date_gmt":"2025-12-21T05:45:17","guid":{"rendered":"https:\/\/fanyiming.life\/?p=14234"},"modified":"2025-12-21T13:46:15","modified_gmt":"2025-12-21T05:46:15","slug":"%e6%94%af%e6%8c%81%e8%bd%ac%e5%bd%95%e9%80%9f%e7%8e%87%e5%bd%b1%e5%93%8d%e6%a0%b8%e9%85%b6%e6%8a%98%e5%8f%a0%e5%81%87%e8%af%b4%e7%9a%84%e8%af%81%e6%8d%ae","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=14234","title":{"rendered":"\u652f\u6301”\u8f6c\u5f55\u901f\u7387\u5f71\u54cd\u6838\u9176\u6298\u53e0”\u5047\u8bf4\u7684\u8bc1\u636e"},"content":{"rendered":"\n
\n

\u6ce8\uff1aELOF1\u51fa\u73b0\u5728stitchR screen\u7684suppressor\uff0c\u800cNELF\u5728activator<\/p>\n<\/blockquote>\n\n\n\n

    \n
  1. \u6838\u5fc3\u7406\u8bba\uff1a\u52a8\u529b\u5b66\u7ade\u4e89\u6a21\u578b \u6765\u81ea https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3254582\/\uff1a “The competition between the transcription speed and rate constants for the transitions between the different nascent structures determines the RNA folding pathway and the end product of folding.” \u7ffb\u8bd1\uff1a\u8f6c\u5f55\u901f\u5ea6\u4e0e\u4e0d\u540c\u65b0\u751f\u7ed3\u6784\u4e4b\u95f4\u8f6c\u6362\u7684\u901f\u7387\u5e38\u6570\u7684\u7ade\u4e89\uff0c\u51b3\u5b9a\u4e86RNA\u6298\u53e0\u9014\u5f84\u548c\u6700\u7ec8\u4ea7\u7269\u3002<\/li>\n\n\n\n
  2. \u6682\u505c\u5bf9\u6298\u53e0\u7684\u76f4\u63a5\u5f71\u54cd \u6765\u81ea https:\/\/pubmed.ncbi.nlm.nih.gov\/36115451\/\uff1a “Pausing events generate valid cotranscriptional conformational rearrangement to protect functional structures and influence the folding pathway.” \u7ffb\u8bd1\uff1a\u6682\u505c\u4e8b\u4ef6\u4ea7\u751f\u6709\u6548\u7684\u5171\u8f6c\u5f55\u6784\u8c61\u91cd\u6392\uff0c\u4fdd\u62a4\u529f\u80fd\u6027\u7ed3\u6784\u5e76\u5f71\u54cd\u6298\u53e0\u9014\u5f84\u3002<\/li>\n\n\n\n
  3. NusA\u52a0\u901f\u6838\u9176\u6298\u53e0\u7684\u76f4\u63a5\u8bc1\u636e \u6765\u81ea https:\/\/www.pnas.org\/content\/96\/17\/9545\uff1a “The presence of the elongation factor NusA accelerates more than 10-fold the transcription-initiated folding of a circularly permutated ribozyme by E. coli RNA polymerase.” \u7ffb\u8bd1\uff1a\u5ef6\u4f38\u56e0\u5b50NusA\u7684\u5b58\u5728\u4f7f\u6838\u9176\u7684\u5171\u8f6c\u5f55\u6298\u53e0\u52a0\u901f\u8d85\u8fc710\u500d\u3002 \u8fd9\u662f\u6700\u76f4\u63a5\u7684\u8bc1\u636e\uff01\u867d\u7136\u662f\u7ec6\u83cc\u7cfb\u7edf\uff0c\u4f46\u539f\u7406\u76f8\u901a\u3002<\/li>\n\n\n\n
  4. glmS\u6838\u9176\u7684\u5171\u8f6c\u5f55\u6298\u53e0 \u6765\u81ea https:\/\/academic.oup.com\/nar\/article\/52\/2\/872\/7449499\uff1a “Transcriptional pausing within P4.1 may increase correct folding of the core\u2026 Under co-transcriptional regulation, sequential folding allows around half of the transcripts to form the native structure after synthesis of the core, which results in early cleavage before the peripheral helices are fully transcribed.” \u7ffb\u8bd1\uff1a\u8f6c\u5f55\u6682\u505c\u53ef\u80fd\u589e\u52a0\u6838\u5fc3\u7684\u6b63\u786e\u6298\u53e0\u2026\u5728\u5171\u8f6c\u5f55\u8c03\u63a7\u4e0b\uff0c\u7ea6\u4e00\u534a\u7684\u8f6c\u5f55\u672c\u5728\u6838\u5fc3\u5408\u6210\u540e\u5f62\u6210\u5929\u7136\u7ed3\u6784\uff0c\u5bfc\u81f4\u5728\u5916\u56f4\u87ba\u65cb\u5b8c\u5168\u8f6c\u5f55\u4e4b\u524d\u5c31\u53d1\u751f\u65e9\u671f\u526a\u5207\u3002<\/li>\n\n\n\n
  5. ELOF1\u4fc3\u8fdb\u5ef6\u4f38\u901f\u7387 \u6765\u81ea https:\/\/www.nature.com\/articles\/s41556-021-00692-z\uff1a “In line with reduced elongation rate, the overall nascent RNA synthesis was also reduced upon ELOF1 depletion.” \u7ffb\u8bd1\uff1a\u4e0e\u5ef6\u4f38\u901f\u7387\u964d\u4f4e\u4e00\u81f4\uff0cELOF1\u6572\u9664\u540e\u6574\u4f53\u65b0\u751fRNA\u5408\u6210\u4e5f\u51cf\u5c11\u4e86\u3002<\/li>\n<\/ol>\n\n\n\n
    \n\n\n\n

    \u7efc\u5408\u89e3\u8bfb<\/p>\n\n\n\n

    \u56e0\u5b50<\/th>\u5bf9Pol II\u7684\u5f71\u54cd<\/th>\u5bf9\u6838\u9176\u6298\u53e0\u7684\u9884\u6d4b<\/th>\u5728StitchR\u4e2d\u7684\u8868\u578b<\/th>\u662f\u5426\u7b26\u5408\uff1f<\/th><\/tr><\/thead>
    NELF<\/td>\u4fc3\u8fdb\u6682\u505c<\/td>\u6682\u505c\u2192\u66f4\u591a\u65f6\u95f4\u6298\u53e0\u2192\u6709\u5229<\/td>Activator\uff08\u6572\u9664\u2192GFP\u2193\uff09<\/td>\u2705<\/td><\/tr>
    ELOF1<\/td>\u4fc3\u8fdb\u5ef6\u4f38<\/td>\u5feb\u901f\u2192\u6298\u53e0\u65f6\u95f4\u4e0d\u8db3\u2192\u4e0d\u5229<\/td>Suppressor\uff08\u6572\u9664\u2192GFP\u2191\uff09<\/td>\u2705<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

    \u6211\u7684\u7ed3\u8bba\u66f4\u65b0<\/p>\n\n\n\n

    GPT\u7684\u5047\u8bf4\u6709\u6587\u732e\u652f\u6301\uff0c\u4f46\u9700\u8981nuance\uff1a<\/p>\n\n\n\n

      \n
    1. \u2705 \u8f6c\u5f55\u901f\u7387\u786e\u5b9e\u5f71\u54cd\u5171\u8f6c\u5f55RNA\u6298\u53e0\u2014\u2014\u8fd9\u6709\u5927\u91cf\u6587\u732e\u652f\u6301<\/li>\n\n\n\n
    2. \u2705 \u6682\u505c\u53ef\u4ee5\u7ed9\u6838\u9176\u66f4\u591a\u65f6\u95f4\u6b63\u786e\u6298\u53e0\u2014\u2014NusA\u52a0\u901f\u6838\u9176\u6298\u53e010\u500d\u7684\u6570\u636e\u5f88\u6709\u8bf4\u670d\u529b<\/li>\n\n\n\n
    3. \u26a0\ufe0f \u4f46\u8fd9\u662f\u6548\u7387\u95ee\u9898\uff0c\u4e0d\u662f\u7edd\u5bf9\u5fc5\u8981\u2014\u2014\u8f6c\u5f55\u5b8c\u6210\u540e\u6838\u9176\u4ecd\u7136\u53ef\u4ee5\u526a\u5207\uff0c\u53ea\u662f\u53ef\u80fd\u6548\u7387\u8f83\u4f4e\u6216\u4ea7\u7269\u5206\u5e03\u4e0d\u540c \u4f60\u4e4b\u524d\u7684\u8d28\u7591\u4ecd\u7136\u6709\u9053\u7406\uff1a\u6838\u9176\u5728\u8f6c\u5f55\u5b8c\u6210\u540e\u4e5f\u80fd\u5de5\u4f5c\u3002\u4f46\u6587\u732e\u8868\u660e\uff0c\u5171\u8f6c\u5f55\u6298\u53e0\u786e\u5b9e\u53ef\u4ee5\u663e\u8457\u63d0\u9ad8\u6548\u7387\u3002 Sources:<\/li>\n<\/ol>\n\n\n\n
        \n
      • https:\/\/www.pnas.org\/content\/96\/17\/9545<\/li>\n\n\n\n
      • https:\/\/academic.oup.com\/nar\/article\/52\/2\/872\/7449499<\/li>\n\n\n\n
      • https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3254582\/<\/li>\n\n\n\n
      • https:\/\/www.nature.com\/articles\/s41556-021-00692-z<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"

        \u8fd9\u7bc7\u6587\u732e\u7b14\u8bb0\u63a2\u8ba8\u4e86\u8f6c\u5f55\u901f\u7387\u5982\u4f55\u5f71\u54cd\u6838\u9176\u5171\u8f6c\u5f55\u6298\u53e0\u7684\u52a8\u529b\u5b66\u7ade\u4e89\u6a21\u578b\u3002\u4f5c\u8005\u6574\u5408\u4e86\u591a\u9879\u5173\u952e\u7814\u7a76\uff0c\u5305\u62ecNusA\u56e0\u5b50\u52a0\u901f\u6298\u53e0\u7684\u76f4\u63a5\u8bc1\u636e\uff0c\u5e76\u7ed3\u5408NELF\u4e0eELOF1\u5728\u5b9e\u9a8c\u4e2d\u7684\u8868\u578b\uff0c\u4e3a\u201c\u8f6c\u5f55\u6682\u505c\u4fc3\u8fdb\u6b63\u786e\u6298\u53e0\u201d\u7684\u5047\u8bf4\u63d0\u4f9b\u4e86\u6709\u529b\u652f\u6301\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-14234","post","type-post","status-publish","format-standard","hentry","category-18"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/14234","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14234"}],"version-history":[{"count":3,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/14234\/revisions"}],"predecessor-version":[{"id":14238,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/14234\/revisions\/14238"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14234"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14234"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14234"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}