{"id":143,"date":"2023-02-18T01:51:22","date_gmt":"2023-02-17T17:51:22","guid":{"rendered":"http:\/\/39.105.184.3\/?p=143"},"modified":"2025-12-21T01:06:34","modified_gmt":"2025-12-20T17:06:34","slug":"crispr%e6%98%af%e6%af%94morpholino%e6%9b%b4%e7%89%9b%e9%80%bc%e7%9a%84%e5%b7%a5%e5%85%b7%e5%90%97","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=143","title":{"rendered":"CRISPR\u662f\u6bd4Morpholino\u66f4\u725b\u903c\u7684\u5de5\u5177\u5417"},"content":{"rendered":"\n<p>\u76f4\u89c9\u4e0a\uff0c\u57fa\u56e0\u6572\u9664\uff08knockout, KO\uff09\u4eff\u4f5b\u662f\u6bd4\u57fa\u56e0\u6291\u5236\uff08knockdown, KD\uff09\u66f4\u201c\u5f7b\u5e95\u201d\u7684\u4e00\u79cd\u7834\u574f\u57fa\u56e0\u8868\u8fbe\u7684\u65b9\u6cd5\u3002\u90a3\u662f\u5426\u610f\u5473\u7740\uff0cKO\u4e00\u4e2a\u57fa\u56e0\u6240\u5f97\u5230\u7684\u7684\u8868\u578b\u4e00\u5b9a\u6bd4KD\u66f4\u52a0\u660e\u663e\u5462\uff1f\u7b54\u6848\u662f\u4e0d\u4e00\u5b9a\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\" \/>\n\n\n\n<p>\u751f\u7269\u5b66\u5bb6\u5bf9\u57fa\u56e0\u529f\u80fd\u7684\u7814\u7a76\uff0c\u5f80\u5f80\u9700\u8981\u5c06\u201c\u8be5\u57fa\u56e0\u6b63\u5e38\u53d1\u6325\u4f5c\u7528\u201d\u7684\u4e2a\u4f53\u4e0e\u201c\u8be5\u57fa\u56e0\u8868\u8fbe\u5f02\u5e38\u201d\u7684\u4e2a\u4f53\u8fdb\u884c\u5bf9\u6bd4\uff0c\u4ece\u5f02\u5e38\u7684\u8868\u578b\uff08phenotype\uff09\u4e2d\u63a8\u65ad\u8fd9\u4e2a\u57fa\u56e0\u7684\u529f\u80fd\u3002\u65e9\u671f\uff0c\u4eba\u4eec\u53ea\u80fd\u4ece\u81ea\u7136\u754c\u968f\u673a\u51fa\u73b0\u3001\u6216\u4eba\u4e3a\u5927\u89c4\u6a21\u8bf1\u5bfc\u7a81\u53d8\u5f97\u5230\u8868\u578b\u5f02\u5e38\u7684\u4e2a\u4f53\u4e2d\u9009\u62e9\u7814\u7a76\u5bf9\u8c61\uff0c\u9274\u5b9a\u5230\u5e95\u662f\u54ea\u4e2a\u6216\u54ea\u4e9b\u57fa\u56e0\u5f02\u5e38\uff08genotype\uff09\u5f15\u8d77\u8fd9\u4e2a\u8868\u578b\u3002\u7a81\u53d8\u5e38\u5e38\u662f\u968f\u673a\u7684\uff0c\u5373\u4fbf\u53ef\u4ee5\u901a\u8fc7\u4e00\u4e9b\u201c\u7b5b\u9009\u201d\u5bcc\u96c6\u5230\u4e0e\u6240\u7814\u7a76\u7684\u529f\u80fd\u76f8\u5173\u7684\u7a81\u53d8\uff0c\u4f46\u8fd9\u4e00\u8fc7\u7a0b\u4ecd\u7136\u5f88\u5927\u7a0b\u5ea6\u4e0a\u662f\u76f2\u4eba\u6478\u8c61\u3002\u8fd9\u79cd\u5148\u770b\u5230\u8868\u578b\uff0c\u518d\u53bb\u7814\u7a76\u548c\u9274\u5b9a\u80cc\u540e\u4e3b\u5bfc\u57fa\u56e0\u7684\u7814\u7a76\u65b9\u6cd5\u88ab\u79f0\u4e3a\u6b63\u5411\u9057\u4f20\u5b66\uff08Forward genetics\uff09\u3002<\/p>\n\n\n\n<p>\u540e\u6765\uff0c\u62e5\u6709\u4e86\u8d8a\u6765\u8d8a\u591a\u53ef\u4ee5\u76f4\u63a5\u5bf9\u57fa\u56e0\u8fdb\u884c\u5e72\u6270\u6216\u7834\u574f\uff0c\u7ee7\u800c\u89c2\u5bdf\u8fd9\u79cd\u5e72\u6270\u6216\u7834\u574f\u5f15\u8d77\u4e86\u54ea\u4e9b\u5f02\u5e38\u8868\u578b\u7684\u5de5\u5177\uff0c\u8fd9\u6837\u7684\u7814\u7a76\u88ab\u79f0\u4e3a\u53cd\u5411\u9057\u4f20\u5b66\uff08Reverse genetics\uff09<a href=\"alert('Didier Stainier\u5728\u591a\u7bc7\u6587\u7ae0\u4e2d\u8ba4\u4e3aKD\u7b97\u4e0d\u4e0a\u53cd\u5411\u9057\u4f20\u5b66\uff0c\u53ea\u6709KO\u624d\u7b97\uff0c\u4e0d\u8fc7\u6211\u6ca1\u770b\u5230\u4ed6\u89e3\u91ca\u4e3a\u4ec0\u4e48\uff0c\u4e5f\u8bb8\u662f\u5b66\u754c\u516c\u8ba4\u3002');\">[1]<\/a>\u3002\u5728\u53d1\u80b2\u751f\u7269\u5b66\u4e2d\uff0c\u4eba\u4eec\u5e38\u4f7f\u7528\u4e00\u79cd\u53ebMorpholino(MO)\u7684\u5de5\u5177\uff0c\u8fd9\u662f\u4e00\u79cd\u6838\u9178\u7c7b\u4f3c\u7269\uff0c\u201c\u5176\u57fa\u672c\u539f\u7406\u662f\u628a\u6838\u82f7\u9178\u4e0a\u9762\u7684\u4e94\u78b3\u7cd6\u73af\u7528\u4e00\u4e2a\u5417\u5549\u73af\uff08morpholino\uff09\u53d6\u4ee3\uff0c\u540c\u65f6\u5bf9\u539f\u6709\u7684\u78f7\u9178\u57fa\u56e2\u4e5f\u505a\u4e86\u6539\u53d8\u3002\u4f7f\u5f97\u8fd9\u6837\u4e00\u4e2aDNA\u5206\u5b50\u7c7b\u4f3c\u7269\u53ef\u4ee5\u4ee5\u78b1\u57fa\u4e92\u8865\u914d\u5bf9\u7684\u65b9\u5f0f\u540cRNA\u548cDNA\u5355\u94fe\u7ed3\u5408\uff0c\u4f46\u662f\u7531\u4e8e\u7ed3\u6784\u7684\u6539\u53d8\uff0c\u4f7f\u5f97\u6574\u4e2a\u5206\u5b50\u4e0d\u5e26\u6709\u4efb\u4f55\u7535\u8377\uff0c\u56e0\u6b64\u65e0\u6cd5\u88ab\u4efb\u4f55\u9176\u6240\u8bc6\u522b\uff0c\u5305\u62ecDNase\u548cRNase. \u4f7f\u5f97\u8fd9\u7c7b\u7269\u8d28\u5728\u7ec6\u80de\u5185\u6709\u7740\u6781\u5f3a\u7684\u7a33\u5b9a\u6027\u3002\u201d<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202302180104258.png?x-oss-process=style\/screenshots\" alt=\"image-20230218010956012\" \/><\/figure>\n\n\n\n<p><a href=\"\/\/www.ncbi.nlm.nih.gov\/probe\/docs\/techmorpholino\/'\">[2]<\/a><\/p>\n\n\n\n<p>Morpholino\u7ed3\u5408\u76ee\u6807mRNA\u540e\uff0c\u963b\u788d\u4e00\u5207\u9176\u5bf9mRNA\u7684\u7ed3\u5408\uff0c\u8fbe\u5230\u6291\u5236\u8868\u8fbe\u6548\u679c\uff0c\u5982\u7ed3\u5408\u5728mRNA\u7684\u4e0a\u6e38\u4f1a\u963b\u788d\u6838\u7cd6\u4f5340S\u4e9a\u57fa\u5411mRNA\u7684\u7ed3\u5408\uff0c\u7ed3\u5408\u5728splice sites\u4f1a\u963b\u788d\u526a\u63a5\uff0c\u7ed3\u5408\u5728miRNA\u9776\u70b9\u6291\u5236miRNA\u7684\u529f\u80fd\u7b49<a href=\"alert('\u88abMorpholino\u7ed3\u5408\u7684mRNA\u4e0d\u4f1a\u88ab\u964d\u89e3\uff0c\u6240\u4ee5\u5f53\u8f6c\u5f55\u51fa\u6765\u7684mRNA\u6570\u91cf\u8d8a\u6765\u8d8a\u591a\u65f6\uff0c\u662f\u6709\u53ef\u80fd\u7ed3\u5408\u5168\u90e8Morpholino\u7684\uff0c\u4e0d\u8fc7\uff0c\u7edd\u5927\u591a\u6570\u60c5\u51b5\u4e0b\uff0c\u90fd\u4f1a\u6ce8\u5c04\u8fc7\u91cf\u7684Morpholino. \u5f53\u7136\uff0c\u6839\u636e\u5b9e\u9a8c\u6240\u9700\uff0c\u4e5f\u53ef\u4ee5\u63a7\u5236MOs\u5b9e\u73b0\u4e00\u5b9a\u201c\u7a0b\u5ea6\u201d\u7684KD\u3002Anti-Sense Oligo(ASO)\u4e5f\u662f\u7814\u7a76\u80da\u80ce\u53d1\u80b2\u4e2d\u5e38\u7528\u7684\u5de5\u5177\u3002ASO\uff08anti-sense oligonucleotides\uff09\u662f\u4e00\u6bb5\u4e0emRNA\u4e92\u8865\u7684DNA\u5e8f\u5217\uff0c\u7ed3\u5408mRNA\u540e\u8bf1\u5bfc\u5176\u88abRNaseH\u964d\u89e3\u3002ASO\u4e0d\u5982Morpholino\u90a3\u4e48\u7a33\u5b9a\uff0c\u4e00\u822c\u4f1a\u52a0\u70b9\u4fee\u9970\u9632\u6b62\u8fc7\u5feb\u964d\u89e3\u3002\u5728\u7814\u7a76\u53d1\u80b2\u65f6\uff0c\u53ef\u4ee5\u901a\u8fc7\u63a7\u5236ASO\u7684\u91cf\uff0c\u5b9e\u73b0\u53ea\u5bf9MBT\u4e4b\u524d\u6bcd\u6e90mRNA\u8fdb\u884c\u964d\u89e3\uff0c\u800c\u5728MBT\u540e\uff0cASO\u964d\u89e3\uff0c\u5408\u5b50\u57fa\u56e0\u7ec4\u4ea7\u751f\u7684mRNA\u4e0d\u53d7\u6291\u5236\u3002');\">[3]<\/a>\u3002Morpholino\u5728\u53d1\u80b2\u751f\u7269\u5b66\u4e2d\u88ab\u5e7f\u6cdb\u5e94\u7528\uff0c\u6700\u5e38\u89c1\u7684\u7528\u6cd5\u662f\u76f4\u63a5\u5411\u80da\u80ce\u4e2d\u6ce8\u5c04MOs\u6765KD\u67d0\u4e2a\u57fa\u56e0\u3002\u6591\u9a6c\u9c7c\u5728\u6851\u6939\u80da\u65f6\u671f\u7684\u5404\u4e2a\u7ec6\u80de\u4e4b\u95f4\u5f62\u6210extensive cytoplasmic bridges\uff0c\u6240\u4ee5\u53ef\u4ee5\u901a\u8fc7\u6ce8\u5c04Morpholino\u5bf9\u5168\u80da\u80ce\u8fdb\u884c\u64cd\u4f5c\u3002\u5728\u540c\u9636\u6bb5\u7684\u722a\u87fe\u80da\u80ce\u4e2d\uff0c\u7ec6\u80de\u8d28\u5206\u88c2\u662f\u5b8c\u5168\u7684\uff0c\u5e26\u6765\u7684\u4e00\u4e2a\u4f18\u52bf\u662f\uff0c\u53ef\u4ee5\u5728\u4e8c\u7ec6\u80de\u671f\u5bf9\u4e00\u4e2a\u7ec6\u80de\u6ce8\u5c04Morpholino\uff0c\u8fd9\u6837\u5c31\u5f97\u5230\u4e00\u4e2a\u7ec6\u80de\u4f5c\u4e3a\u5b9e\u9a8c\u7ec4\uff0c\u53e6\u4e00\u4e2a\u4f5c\u4e3a\u5bf9\u7167\u7ec4<a href=\"\/\/doi.org\/10.1007\/978-1-4939-9732-9_21'\">[4]<\/a>\u3002<\/p>\n\n\n\n<p>\u5982\u6b64\u4e00\u6765\uff0c\u53d1\u80b2\u751f\u7269\u5b66\u5bb6\u83b7\u5f97\u4e86\u8d8a\u6765\u8d8a\u591a\u91cd\u8981\u57fa\u56e0KD\u540e\u7684\u8868\u578b\uff0c\u4e5f\u501f\u6b64\u9610\u660e\u4e86\u8bb8\u591a\u53d1\u80b2\u8fc7\u7a0b\u80cc\u540e\u7684\u5206\u5b50\u57fa\u7840\u3002\u7136\u800c\uff0cMOs\u5e76\u4e0d\u662f\u5b8c\u7f8e\u7684\uff0c\u5176off-target\u6548\u5e94\u4e00\u76f4\u662f\u4e00\u4e2a\u5f88\u5927\u7684\u95ee\u9898\uff0c\u5373\u6211\u4eec\u770b\u5230\u7684\u8868\u578b\u53ef\u80fd\u5e76\u4e0d\u662f\u56e0\u4e3a\u76ee\u7684\u57fa\u56e0\u88ab\u6572\u964d\u5bfc\u81f4\u7684\uff0c\u800c\u662f\u56e0\u4e3a\u4e00\u4e2a\u4e0e\u8bfe\u9898\u5b8c\u5168\u4e0d\u76f8\u5e72\u7684\u57fa\u56e0\u88ab\u610f\u5916\u5730\u6572\u964d\u800c\u9020\u6210\u3002\u4e00\u4e2a\u7ecf\u9a8c\u662f\u201c25-mer\u7684MO\u4e2d\u67094\u4e2a\u9519\u914d\u4f9d\u7136\u53ef\u4ee5\u5728\u9ad8\u6d53\u5ea6\u4e0b\u8fbe\u5230\u76f8\u540c\u7684\u6548\u679c\u201d<a href=\"\/\/pubmed.ncbi.nlm.nih.gov\/18403413\/'\">[5]<\/a>\u3002<\/p>\n\n\n\n<p>\u518d\u540e\u6765\uff0c\u66f4\u725b\u903c\u7684\u5de5\u5177\u6765\u4e86\uff0cTALEN\u548cCRISPR\/Cas9\u7b49\u771f\u6b63\u5b9e\u73b0\u4e86\u5f7b\u5e95\u4ece\u57fa\u56e0\u7ec4\u4e0a\u201c\u6572\u9664\u201d\u4e00\u4e2a\u57fa\u56e0\u3002\u65b0\u5de5\u5177\u5982\u6b64\u7b80\u4fbf\u597d\u7528\uff0c\u800cMOs\u7684off-target\u98ce\u9669\u4e00\u76f4\u4e0d\u53ef\u5ffd\u89c6\uff0c\u4e1a\u5185\u4eba\u58eb\u5f00\u59cb\u8bae\u8bba\uff0c\u662f\u5426\u8981\u653e\u5f03MOs\u7684\u4e3b\u6d41\u5730\u4f4d\uff0c\u751a\u81f3\u5728\u671f\u520a\u5ba1\u6838\u65f6\u80fd\u7528CRISPR\/Cas9\u7684\u5730\u65b9\u5fc5\u987b\u8981\u6c42\u4f7f\u7528CRIPSR\/Cas9\uff0c\u800c\u4e0d\u80fd\u53ea\u63d0\u4f9bMOs\u7684\u6570\u636e<a href=\"alert('\u8fd9\u6bb5\u662f\u6211\u8f6e\u8f6c\u8001\u677f\uff08\u4f5c\u4e3a\u4e1a\u5185\u8001\u4eba\uff09\u53e3\u8ff0\u7684\u53d1\u751f\u8fc7\u7684\u4e89\u8bae\u3002');\">[6]<\/a>\u3002<\/p>\n\n\n\n<p>\u6682\u4e14\u4e0d\u8bba\u5708\u5b50\u5185\u662f\u5426\u5c31Morpholino\u7684\u53bb\u7559\u8fbe\u6210\u5171\u8bc6\uff0c\u4f7f\u7528TALEN\u548cCRISPR\/Cas9\u6572\u9664\u57fa\u56e0\u7684\u6570\u636e\u4e0d\u53ef\u62b5\u6321\u5730\u5728\u79ef\u7d2f\u3002\u4e8e\u662f\uff0c\u5bf9\u4e8e\u540c\u4e00\u4e2a\u57fa\u56e0\uff0c\u65e2\u6709\u901a\u8fc7\u4f20\u7edfMOs\uff08\u6216Anti-sense Oligonucleotides<a href=\"alert('Anti-Sense Oligo(ASO)\u4e5f\u662f\u7814\u7a76\u80da\u80ce\u53d1\u80b2\u4e2d\u5e38\u7528\u7684\u5de5\u5177\u3002ASO\uff08anti-sense oligonucleotides\uff09\u662f\u4e00\u6bb5\u4e0emRNA\u4e92\u8865\u7684DNA\u5e8f\u5217\uff0c\u7ed3\u5408mRNA\u540e\u8bf1\u5bfc\u5176\u88abRNaseH\u964d\u89e3\u3002ASO\u4e0d\u5982Morpholino\u90a3\u4e48\u7a33\u5b9a\uff0c\u4e00\u822c\u4f1a\u52a0\u70b9\u4fee\u9970\u9632\u6b62\u8fc7\u5feb\u964d\u89e3\u3002\u5728\u7814\u7a76\u53d1\u80b2\u65f6\uff0c\u53ef\u4ee5\u901a\u8fc7\u63a7\u5236ASO\u7684\u91cf\uff0c\u5b9e\u73b0\u53ea\u5bf9MBT\u4e4b\u524d\u6bcd\u6e90mRNA\u8fdb\u884c\u964d\u89e3\uff0c\u800c\u5728MBT\u540e\uff0cASO\u964d\u89e3\uff0c\u5408\u5b50\u57fa\u56e0\u7ec4\u4ea7\u751f\u7684mRNA\u4e0d\u53d7\u6291\u5236\u3002');\">[3]<\/a>\u7b49\uff09\u5de5\u5177KD\u7684\u6570\u636e\uff0c\u4e5f\u6709\u4f7f\u7528CRISPR\/Cas9\u7b49KO\u7684\u6570\u636e\uff0c\u8fd9\u4e24\u5957\u7ed3\u679c\u81ea\u7136\u4f1a\u88ab\u653e\u5728\u4e00\u8d77\u5bf9\u6bd4\u3002\u7406\u60f3\u60c5\u51b5\u4e0b\uff0c\u5f7b\u5e95\u6c89\u9ed8\u6389\u4e00\u4e2a\u57fa\u56e0\uff0c\u548c\u6572\u9664\u4e00\u4e2a\u57fa\u56e0\uff0c\u7ed3\u679c\u90fd\u662f\u9020\u6210\u8be5\u57fa\u56e0\u8868\u8fbe\u7684\u86cb\u767d\u8d28\u4e0d\u80fd\u6b63\u5e38\u4ea7\u751f\u5e76\u53d1\u6325\u4f5c\u7528\uff0c\u90a3\u4e48\u5f15\u8d77\u7684\u5b8f\u89c2\u4e2a\u4f53\u6c34\u5e73\u4e0a\u7684\u8868\u578b\u5c31\u5e94\u8be5\u662f\u4e00\u6837\u7684\u3002\u54ea\u6015\u4e0d\u4e00\u6837\uff0cKO\u4ece\u6e90\u5934\u4e0a\u6539\u53d8DNA\u5e8f\u5217\uff0c\u4e5f\u5e94\u8be5\u66f4\u201c\u5f7b\u5e95\u201d\uff0c\u4ea7\u751f\u66f4\u4e25\u91cd\u7684phenotype\u3002<\/p>\n\n\n\n<p>\u4ee4\u4eba\u548b\u820c\u7684\u662f\uff0c\u4e8b\u5b9e\u8ddf\u6211\u4eec\u9884\u8ba1\u7684\u6070\u597d\u76f8\u53cd\uff0cKO\u5f97\u5230\u7684\u8868\u578b\u5f80\u5f80\u6bd4KD\u5f97\u5230\u7684\u8868\u578b\u66f4\u201c\u8f7b\u5fae\u201d\uff0c\u66f4\u6709\u751a\u8005\u51e0\u4e4e\u770b\u4e0d\u5230\u4e0e\u91ce\u751f\u578b\u7684\u5dee\u522b\u3002\u8fd9\u6837\u7684\u4f8b\u5b50\u5982\u6b64\u4e4b\u591a\uff0c\u4ece\u722a\u87fe\u3001\u6591\u9a6c\u9c7c\u3001\u5c0f\u9f20\u5230\u4eba\u4e2d\u90fd\u6709\u8fd9\u6837\u7684\u4e8b\u60c5\u88ab\u6587\u732e\u62a5\u9053\u51fa\u6765\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202302180105495.png?x-oss-process=style\/screenshots\" alt=\"image-20230218010553440\" \/><\/figure>\n\n\n\n<p>\u4e3e\u4e2a\u4f8b\u5b50\uff0c\u4e0a\u56fe\u4e2d<em>Egfl7<\/em>\u662f\u4e2a\u5728\u6591\u9a6c\u9c7c\u5fc3\u8840\u7ba1\u53d1\u80b2\u4e2d\u8d77\u4f5c\u7528\u7684\u57fa\u56e0\uff0c\u5de6\u8fb9\u662f\u7528Anti-sense Oligonulceotides (ASO)\u5bf9\u8fd9\u4e2a\u57fa\u56e0\u8fdb\u884cKD\uff0c\u53ef\u4ee5\u770b\u5230AS\u7ec4\u5bf9\u6bd4Con\u7ec4\uff08control\uff09\u53d1\u751f\u660e\u663e\u7684\u8840\u7ba1\u53d1\u80b2\u5f02\u5e38\uff08\u8bf4\u4eba\u8bdd\u5c31\u662f\u4f60\u770bAS\u91cc\u90a3\u4e2a\u67d3\u6210\u84dd\u8272\u7684\u8840\u7ba1\u6bd4Con\u7ec6\u4e86\uff0c\u800c\u4e14\u4fa7\u679d\u90fd\u6d88\u5931\u4e86\uff0c\u54b1\u4e0d\u61c2\uff0c\u4f46\u4e5f\u80fd\u770b\u51fa\u6765\u662f\u4e2a\u4e25\u91cd\u7684\u5f02\u5e38\u8868\u578b)\uff0c\u53f3\u56fe\u662f\u7528CRIPSR\/Cas9\u5bf9<em>Egfl7<\/em>\u8fdb\u884cKO\uff0c\u53ef\u4ee5\u770b\u5230mCherry\u6807\u8bb0\u7684\u533a\u57df\u8ddfWT\u6839\u672c\u770b\u4e0d\u51fa\u6765\u5dee\u5f02\uff0c\u552f\u4e00\u7684\u4e00\u70b9\u70b9\u533a\u522b\u662f\u53f3\u4e0a\u89d2egfl7-\/-\u7684\u8111\u95e8\u90a3\u91cc\u6709\u4e00\u70b9\u7ea2\uff0c\u4f46\u5bf9\u6bd4\u5de6\u56fe\u7684\u8868\u578b\u5c31\u8f7b\u5fae\u5f97\u591a\u4e86<a href=\"text=The%20genetic%20compensation%20response%20%28GCR%29%20was%20firstly%20described,can%20assume%20the%20function%20of%20the%20mutated%20gene.'\">[7]<\/a>\u3002<\/p>\n\n\n\n<p>\u7a76\u7adf\u662f\u4ec0\u4e48\u539f\u56e0\uff0c\u5bfc\u81f4\u4e8b\u5b9e\u8ddf\u60f3\u8c61\u4e0d\u4e00\u6837\u5462\uff1f\u5927\u81f4\u731c\u60f3\u51e0\u79cd\u53ef\u80fd<a href=\"\/\/pubmed.ncbi.nlm.nih.gov\/29049395\/'\">[8]<\/a>\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>KD\u7684\u9776\u6807\u662fmRNA\uff0c\u800cKO\u7684\u9776\u6807\u662f\u57fa\u56e0\u7ec4\u3002\u800c\u5bf9\u4e8e\u80da\u80ce\u800c\u8a00\uff0c\u76f4\u5230MBT\uff08Mid-Blastula Transition\uff0c\u4e2d\u56ca\u80da\u671f\u8f6c\u6362\uff09\u4e4b\u540e\uff0c\u5408\u5b50\u57fa\u56e0\u624d\u5f00\u59cb\u8868\u8fbe\uff0c\u4e4b\u524d\u7684\u6bcd\u6e90mRNA\u7684\u8868\u8fbe\u5e76\u4e0d\u4f1a\u53d7\u5230KO\u7684\u5f71\u54cd\uff0c\u56e0\u6b64\uff0c\u5982\u679c\u80da\u80ce\u5185\u62e5\u6709\u4e00\u4e2a\u57fa\u56e0\u7684\u6bcd\u6e90mRNA\uff0cKO\u7684\u8868\u578b\u5c31\u4f1a\u66f4\u5f31\u3002<\/li>\n\n\n\n<li>\u5982\u524d\u6240\u8ff0\uff0cMOs\u7684\u8131\u9776\u6548\u5e94\u3002<\/li>\n\n\n\n<li>KO\u65f6\u9020\u6210\u7684\u7a81\u53d8\u5e76\u6ca1\u6709\u5b8c\u5168\u4f7f\u5b83\u5931\u6d3b\uff0c\u800c\u662f\u4fdd\u7559\u4e00\u90e8\u5206\u6d3b\u6027\uff0c\u6210\u4e3ahypomorphic allele <a href=\"alert('Your physical traits are largely the result of your chromosomes. You have 23 pairs of chromosomes, one pair member from each parent. About 2 percent of your chromosomes are portions that code for proteins and are called genes. Since you have two of each chromosome, you have two of each gene. Gene pairs are called alleles, and a hypomorphic allele reduces how well the gene expresses itself.');\">[9]<\/a>.<\/li>\n\n\n\n<li>\u56e0\u4e3a\u67d0\u4e9b\u4e0d\u53ef\u77e5\u7684\u539f\u56e0\uff0cmutant\u4e2d\u53d1\u751f\u4e86\u4e00\u4e9b\u9057\u4f20\u8865\u507f\uff08genetic compensation\uff09\uff0c\u800cmorphant<a href=\"alert('\u884c\u8bdd\uff0cKO\u7684\u4e2a\u4f53\u53ebmutant\uff0c\u88abMOs KD\u7684\u4e2a\u4f53\u53ebmorphant.');\">[10]<\/a>.\u4e2d\u6ca1\u6709\u53d1\u751f\u3002<\/li>\n<\/ol>\n\n\n\n<p>Didier Stainier\u662f\u9a6c\u666e\u6240\u7684\u4e00\u540d\uff08\u5927\u4f6c\uff09\u7814\u7a76\u5458\uff0c\u662f\u56fd\u9645\u4e00\u4e2a\u7814\u7a76zebrafish\u7684\u4ec0\u4e48\u4ec0\u4e48\u534f\u4f1a\u7684\u4e3b\u5e2d\uff0c\u4f5c\u4e3aleader\uff0c\u6709\u8d23\u4efb\u56de\u7b54\u4e00\u4e0b\u5e74\u4f1a\u4e0a\u4f1a\u5458\u4eec\u7684\u4e89\u8bae\u95ee\u9898<a href=\"alert('\u8111\u8865\u7684');\">[11]<\/a>\uff0c\u4e8e\u662f\u627e\u4e2a\u51e0\u4e2a\u7814\u7a76\u7272\u5bf9\u8fd9\u4e2a\u95ee\u9898\u5f00\u5c55\u4e86\u7814\u7a76<a href=\"\/\/pubmed.ncbi.nlm.nih.gov\/30944477\/'\">[12]<\/a>\u3002<\/p>\n\n\n\n<p>\u7b80\u800c\u8a00\u4e4b\uff0c\u4ed6\u53d1\u73b0\u4ed6\u627e\u7684\u90a3\u51e0\u4e2a\u57fa\u56e0\uff08\u8868\u73b0\u51faKD\u8868\u578b\u6bd4KO\u66f4\u660e\u663e\u7684\uff09\u2014\u2014\u6709\u4e00\u4e2a\u5171\u540c\u7684\u7279\u70b9\uff0c\u5728\u5b83\u4eec\u88abCRISPR\/Cas9 KO\u540e\uff0c\u57fa\u56e0\u7ec4\u4e2d\u4e0e\u5b83\u4eec\u5e8f\u5217\u76f8\u4f3c\u7684\u57fa\u56e0\uff08\u6700\u76f8\u4f3c\u7684\u90a3\u90e8\u5206\u57fa\u56e0\u5c31\u662f\u5b83\u7684paralogs\u2014\u65c1\u7cfb\u540c\u6e90\u57fa\u56e0\uff09\u7684\u8868\u8fbe\u91cf\u4f1a\u4e0a\u5347\uff0c\u56e0\u4e3a\u5e8f\u5217\u76f8\u4f3c\u5728\u6982\u7387\u4e0a\u529f\u80fd\u4e5f\u76f8\u4f3c\uff0c\u6240\u4ee5\u5c31\u201c\u56de\u8865\u201d\u4e86\u4e00\u4e9b\u8be5\u57fa\u56e0KO\u9020\u6210\u7684\u8868\u578b\u5f02\u5e38\uff0c\u5982\u4e0b\u56fe\u7684<em>Egfl7<\/em>\u548c<em>emilin<\/em>\u5bb6\u65cf\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202302180106241.png?x-oss-process=style\/screenshots\" alt=\"image-20230218010956012\" \/><\/figure>\n\n\n\n<p><a href=\"alert('Egfl7\u548cemilin3a\u7528NW\u7b97\u6cd5\u53cc\u5e8f\u5217\u6bd4\u5bf9\u7684\u76f8\u4f3c\u5ea6\u7ea640%\u3002\u56fe\u4e2dMO\u4ee3\u8868Morpholino\uff0cNT\u548cT\u662f\u5206\u522b\u5bf9\u975e\u6a21\u677f\u94fe\u548c\u6a21\u677f\u94feCRIPSRi.');\">[13]<\/a><\/p>\n\n\n\n<p>\u4ed6\u8fd8\u53d1\u73b0\uff0c\u540c\u4e00\u4e2a\u57fa\u56e0\u7528CRISPR\/Cas9 KO\uff0c\u6709\u7684mutant\u7684paralogs\u8868\u8fbe\u4e0a\u8c03\uff0c\u6709\u7684\u5219\u4e00\u70b9\u90fd\u4e0d\u4e0a\u8c03\u3002\u533a\u522b\u5728\u4e8e\uff0c\u524d\u8005\u88abKO\u7684\u57fa\u56e0\u7684mRNA\u88ab\u5927\u91cfdecay\uff0c\u540e\u8005\u6ca1\u6709\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202302180107539.png\" alt=\"image-20230218010956012\" \/><\/figure>\n\n\n\n<p>\u6240\u4ee5\u4ed6\u7684\u7ed3\u8bba\u662f\u2014\u2014KO\u5728mutant\u4e2d\u4ea7\u751f\u4e00\u6761\u65e0\u529f\u80fd\u7684mRNA\uff0c\u88abRNA surveillance\u7cfb\u7edf\u76d1\u6d4b\u5230\u4ee5\u540e\u88ab\u7ec6\u80de\u4e3b\u52a8decay\u6389\uff0c\u8fd9\u4e2adecay\u8fc7\u7a0b\u8bf1\u53d1\u4e0e\u5b83\u5e8f\u5217\u76f8\u4f3c\u7684\u57fa\u56e0\u8868\u8fbe\u91cf\u4e0a\u8c03\uff0c\u8fd9\u4e9b\u57fa\u56e0\u4e2d\u5982\u679c\u5305\u62ec\u4e00\u4e9b\u529f\u80fd\u4e5f\u7c7b\u4f3c\u7684\u57fa\u56e0\uff0c\u5c31\u8865\u507f\u4e86KO\u9020\u6210\u7684\u8868\u578b\u5f02\u5e38\u3002\u4ed6\u8fd8\u53d1\u660e\u4e86\u4e00\u4e2a\u8bcd\u6765\u63cf\u8ff0\u4ed6\u53d1\u73b0\u7684\u8fd9\u4e2a\u8fc7\u7a0b\u2014\u2014\u8f6c\u5f55\u9002\u5e94\uff08Transcriptional adaptation\uff09<a href=\"alert('\u95ee\u9898\u5728\u4e8e\uff0c\u54ea\u79cd\u7c7b\u578b\u7684mRNA decay\u4f1a\u9020\u6210Transcriptional adaptation\uff0c\u54ea\u79cd\u53c8\u4e0d\u4f1a\u3002\u663e\u7136\u4e0d\u662f\u6240\u6709\u7684mRNA decay\u90fd\u4f1a\u9020\u6210transcriptional adaptation. \u7528antisense oligonucleotides\u6765KD\u4e5f\u4f1a\u5f15\u8d77RNaseH\u7b49\u5bf9mRNA-DNA\u6742\u4ea4\u94fe\u8fdb\u884c\u6d88\u5316\uff0c\u6216\u5f15\u8d77DICER\u548cAGO\u4ecb\u5bfc\u7684RNAi\u7cfb\u7edf\u3002mRNA decay\u7684\u5206\u7c7b\u548c\u89e6\u53d1\u6761\u4ef6\u6709\u54ea\u4e9b\u5462\uff1f');\">[14]<\/a>\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202302180109363.png?x-oss-process=style\/screenshots\" alt=\"image-20230218010907821\" \/><\/figure>\n\n\n\n<p>\u8fd9\u4e2a\u7814\u7a76\u81f3\u5c11\u662f\u90e8\u5206\u89e3\u91ca\u4e86KO\u8868\u578b\u8f7b\u4e8eKD\u7684\u73b0\u8c61\uff0c\u4f46\u663e\u7136\u662f\u4e0d\u5b8c\u5168\u7684\u3002Transcriptional adaptation\u53ea\u662fCompensation\u7684\u4e00\u79cd\u5f62\u5f0f\uff0c\u751f\u7269\u8fd8\u6709\u66f4\u591a\u7684compensation\u7684\u673a\u5236\u4ea4\u7ec7\u5728\u4e00\u8d77\u3002<\/p>\n\n\n\n<p>\u65e9\u5728\u8fd9\u7bc7\u6587\u7ae0\u53d1\u8868\u4ee5\u524d\uff0c\u4eba\u4eec\u65e9\u5c31\u6000\u7591\u53ef\u80fd\u662fparalogs\u4e0a\u8c03\u5bf9mutant\u7684\u8868\u578b\u8fdb\u884c\u4e86compensation. 2017\u5e74\u6709\u7bc7\u6587\u7ae0\u9898\u76ee\u5c31\u53eb\u201cComparison of Zebrafish <em>tmem88a<\/em> mutant and morpholino knockdown phenotypes\u201d<a href=\"\/\/pubmed.ncbi.nlm.nih.gov\/28192479\/'\">[15]<\/a>\uff0c\u6bd4\u8f83\u6591\u9a6c\u9c7c<em>tmem88a<\/em>\u7684mutant\u548cmorpholino KD\u7684\u8868\u578b\u3002\u4f5c\u8005\u4e5f\u662f\u53d1\u73b0mutant\u7684phenotype\u663e\u8457\u5f31\u4e8emorphant\uff0c\u4ed6\u4eec\u4e5f\u6000\u7591\u662f\u4e0d\u662f\u6709paralogs\u4e0a\u8c03\u4e86\uff0c\u4f46\u505a\u4e86\u5b9e\u9a8c\u53d1\u73b0\u5e76\u6ca1\u6709\u3002\u5728conclusion\u4e2d\uff0c\u4ed6\u4eec\u628a\u8fd9\u5dee\u5f02\u5f52\u7ed3\u5230MOs\u7684\u8131\u9776\u6548\u5e94\u4e0a\u3002\u4f46\u663e\u7136\u8fd9\u4ecd\u7136\u662f\u731c\u6d4b\uff0ccompensation\u8fdc\u6bd4\u6211\u4eec\u60f3\u8c61\u7684\u8981\u590d\u6742\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202302180110587.png?x-oss-process=style\/screenshots\" alt=\"image-20230218010956012\" \/><\/figure>\n\n\n\n<p>\u603b\u4e4b\uff0c\u6280\u672f\u90fd\u6709\u4f18\u7f3a\u70b9\uff0cMOs\u81f3\u4eca\u4ecd\u7136\u5730\u4f4d\u4e0d\u53ef\u64bc\u52a8\uff0cCRISPR\/Cas9\u5982\u65e5\u4e2d\u5929\u7684\u540c\u65f6\uff0c\u8bf8\u5982compensation\u4e4b\u7c7b\u7684\u7f3a\u70b9\u4e5f\u66b4\u9732\u51fa\u6765\u3002\u5f53\u7136\u5c31\u7b97\u4e0d\u8c08\u8fd9\u4e9b\uff0cknockdown\u4e5f\u5728\u5f88\u591a\u573a\u666f\u4e0b\u662f\u4e0d\u53ef\u4ee3\u66ff\u7684\u3002\u54b1\u4eec\u597d\u597d\u5b66\u6df1\u5b66\u900f\u3001\u6d3b\u5b66\u6d3b\u7528\u624d\u662f\u771f\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\" \/>\n\n\n\n<p>[1] Didier Stainier\u5728\u591a\u7bc7\u6587\u7ae0\u4e2d\u8ba4\u4e3aKD\u7b97\u4e0d\u4e0a\u53cd\u5411\u9057\u4f20\u5b66\uff0c\u53ea\u6709KO\u624d\u7b97\uff0c\u4e0d\u8fc7\u6211\u6ca1\u770b\u5230\u4ed6\u89e3\u91ca\u4e3a\u4ec0\u4e48\uff0c\u4e5f\u8bb8\u662f\u5b66\u754c\u516c\u8ba4\u3002<\/p>\n\n\n\n<p>[2] \u4ec0\u4e48\u662fmorpholino\uff1f<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/probe\/docs\/techmorpholino\/\">https:\/\/www.ncbi.nlm.nih.gov\/probe\/docs\/techmorpholino\/<\/a><\/p>\n\n\n\n<p>[3] \u88abMorpholino\u7ed3\u5408\u7684mRNA\u4e0d\u4f1a\u88ab\u964d\u89e3\uff0c\u6240\u4ee5\u5f53\u8f6c\u5f55\u51fa\u6765\u7684mRNA\u6570\u91cf\u8d8a\u6765\u8d8a\u591a\u65f6\uff0c\u662f\u6709\u53ef\u80fd\u7ed3\u5408\u5168\u90e8Morpholino\u7684\uff0c\u4e0d\u8fc7\uff0c\u7edd\u5927\u591a\u6570\u60c5\u51b5\u4e0b\uff0c\u90fd\u4f1a\u6ce8\u5c04\u8fc7\u91cf\u7684Morpholino. \u5f53\u7136\uff0c\u6839\u636e\u5b9e\u9a8c\u6240\u9700\uff0c\u4e5f\u53ef\u4ee5\u63a7\u5236MOs\u5b9e\u73b0\u4e00\u5b9a\u201c\u7a0b\u5ea6\u201d\u7684KD\u3002Anti-Sense Oligo(ASO)\u4e5f\u662f\u7814\u7a76\u80da\u80ce\u53d1\u80b2\u4e2d\u5e38\u7528\u7684\u5de5\u5177\u3002ASO\uff08anti-sense oligonucleotides\uff09\u662f\u4e00\u6bb5\u4e0emRNA\u4e92\u8865\u7684DNA\u5e8f\u5217\uff0c\u7ed3\u5408mRNA\u540e\u8bf1\u5bfc\u5176\u88abRNaseH\u964d\u89e3\u3002ASO\u4e0d\u5982Morpholino\u90a3\u4e48\u7a33\u5b9a\uff0c\u4e00\u822c\u4f1a\u52a0\u70b9\u4fee\u9970\u9632\u6b62\u8fc7\u5feb\u964d\u89e3\u3002\u5728\u7814\u7a76\u53d1\u80b2\u65f6\uff0c\u53ef\u4ee5\u901a\u8fc7\u63a7\u5236ASO\u7684\u91cf\uff0c\u5b9e\u73b0\u53ea\u5bf9MBT\u4e4b\u524d\u6bcd\u6e90mRNA\u8fdb\u884c\u964d\u89e3\uff0c\u800c\u5728MBT\u540e\uff0cASO\u964d\u89e3\uff0c\u5408\u5b50\u57fa\u56e0\u7ec4\u4ea7\u751f\u7684mRNA\u4e0d\u53d7\u6291\u5236\u3002<\/p>\n\n\n\n<p>[4] Bestman, J.E., Cline, H.T. (2020). Morpholino Studies in <em>Xenopus<\/em> Brain Development. In: Sprecher, S. (eds) Brain Development. Methods in Molecular Biology, vol 2047. Humana, New York, NY. https:\/\/doi.org\/10.1007\/978-1-4939-9732-9_21<\/p>\n\n\n\n<p>[5] Eisen JS, Smith JC. Controlling morpholino experiments: don&#8217;t stop making antisense. Development. 2008 May;135(10):1735-43. doi: 10.1242\/dev.001115. Epub 2008 Apr 9. PMID: 18403413.<\/p>\n\n\n\n<p>\u8bbe\u7f6e\u5145\u5206\u7684\u5bf9\u7167\uff0c\u4ee5\u6700\u5927\u7a0b\u5ea6\u6392\u9664off-target\u5bf9\u5b9e\u9a8c\u9020\u6210\u7684\u5e72\u6270\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5bf9\u7167\u7ec4\u8bbe\u8ba1<\/li>\n\n\n\n<li>\u672c\u7269\u79cd\u6ca1\u6709\u7684\u201c\u5916\u6e90\u201d\u57fa\u56e0\u7684MOs\uff08\u5c3d\u91cf\u9009\u62e9\u548c\u9776\u57fa\u56e0\u66f4\u50cf\u7684\uff09\u4f5c\u4e3a\u9634\u6027\u5bf9\u7167<\/li>\n\n\n\n<li>\u8bbe\u8ba1MO\u524d\u4f7f\u7528Blast\uff0c\u5c3d\u91cf\u4e0d\u9009\u62e9\u8ddf\u5176\u4ed6\u57fa\u56e0\u6709\u76f8\u4f3c\u5e8f\u5217\u7684\u533a\u57df<\/li>\n\n\n\n<li>\u4f7f\u7528\u4e0d\u5b8c\u7f8e\u914d\u5bf9\u7684MOs<\/li>\n\n\n\n<li>\u4f7f\u75285\u4e2a\u6838\u82f7\u9178\u9519\u914d\u7684MO\u4f5c\u4e3a\u5bf9\u7167<\/li>\n\n\n\n<li>\u4f7f\u7528\u4e0d\u540cMOs\u9776\u5411\u540c\u4e00\u4e2a\u57fa\u56e0<\/li>\n\n\n\n<li>Rescue<\/li>\n\n\n\n<li>\u8fd9\u662f\u6700\u53ef\u9760\u7684control<\/li>\n\n\n\n<li>\u4ee5\u4e00\u79cd\u5bf9MO\u514d\u75ab\u7684\u5f62\u5f0f\u56de\u8865\u8fd9\u4e2a\u88abMO\u6572\u964d\u7684\u57fa\u56e0<\/li>\n\n\n\n<li>\u5bf9\u4e8e\u666e\u904d\u9ad8\u8868\u8fbe\u7684\u57fa\u56e0\u6765\u8bf4\uff0crescue\u76f8\u5bf9\u5bb9\u6613\uff1b\u4f46\u5982\u679c\u8be5\u57fa\u56e0\u539f\u672c\u7684\u8868\u8fbe\u91cf\u662f\u7cbe\u786e\u63a7\u5236\u7684\uff0c\u5c31\u5f88\u96be\u4eba\u4e3arescue.<\/li>\n<\/ol>\n\n\n\n<p>[6] \u8fd9\u6bb5\u662f\u6211\u8f6e\u8f6c\u8001\u677f\uff08\u4f5c\u4e3a\u4e1a\u5185\u8001\u4eba\uff09\u53e3\u8ff0\u7684\u53d1\u751f\u8fc7\u7684\u4e89\u8bae\u3002<\/p>\n\n\n\n<p>[7] Rossi A, Kontarakis Z, Gerri C, Nolte H, H\u00f6lper S, Kr\u00fcger M, Stainier DY. Genetic compensation induced by deleterious mutations but not gene knockdowns. Nature. 2015 Aug 13;524(7564):230-3. doi: 10.1038\/nature14580. Epub 2015 Jul 13. PMID: 26168398.<\/p>\n\n\n\n<p>[8] Stainier DYR, Raz E, Lawson ND, Ekker SC, Burdine RD, Eisen JS, Ingham PW, Schulte-Merker S, Yelon D, Weinstein BM, Mullins MC, Wilson SW, Ramakrishnan L, Amacher SL, Neuhauss SCF, Meng A, Mochizuki N, Panula P, Moens CB. Guidelines for morpholino use in zebrafish. PLoS Genet. 2017 Oct 19;13(10):e1007000. doi: 10.1371\/journal.pgen.1007000. PMID: 29049395; PMCID: PMC5648102.<\/p>\n\n\n\n<p>[9] What is &#8220;Hypomorphic&#8221;? <a href=\"https:\/\/education.seattlepi.com\/hypomorphic-allele-4565.html\">https:\/\/education.seattlepi.com\/hypomorphic-allele-4565.html<\/a><\/p>\n\n\n\n<p>[10] \u884c\u8bdd\uff0cKO\u7684\u4e2a\u4f53\u53ebmutant\uff0c\u88abMOs KD\u7684\u4e2a\u4f53\u53ebmorphant.<\/p>\n\n\n\n<p>[11] \u8111\u8865\u7684\u3002<\/p>\n\n\n\n<p>[12] El-Brolosy MA, Kontarakis Z, Rossi A, Kuenne C, G\u00fcnther S, Fukuda N, Kikhi K, Boezio GLM, Takacs CM, Lai SL, Fukuda R, Gerri C, Giraldez AJ, Stainier DYR. Genetic compensation triggered by mutant mRNA degradation. Nature. 2019 Apr;568(7751):193-197. doi: 10.1038\/s41586-019-1064-z. Epub 2019 Apr 3. PMID: 30944477; PMCID: PMC6707827.<\/p>\n\n\n\n<p>[13] <em>Egfl7<\/em>\u548c<em>emilin3a<\/em>\u7528NW\u7b97\u6cd5\u53cc\u5e8f\u5217\u6bd4\u5bf9\u7684\u76f8\u4f3c\u5ea6\u7ea640%\u3002\u56fe\u4e2dMO\u4ee3\u8868Morpholino\uff0cNT\u548cT\u662f\u5206\u522b\u5bf9\u975e\u6a21\u677f\u94fe\u548c\u6a21\u677f\u94feCRIPSRi<\/p>\n\n\n\n<p>[14] \u95ee\u9898\u5728\u4e8e\uff0c\u54ea\u79cd\u7c7b\u578b\u7684mRNA decay\u4f1a\u9020\u6210Transcriptional adaptation\uff0c\u54ea\u79cd\u53c8\u4e0d\u4f1a\u3002\u663e\u7136\u4e0d\u662f\u6240\u6709\u7684mRNA decay\u90fd\u4f1a\u9020\u6210transcriptional adaptation. \u7528antisense oligonucleotides\u6765KD\u4e5f\u4f1a\u5f15\u8d77RNaseH\u7b49\u5bf9mRNA-DNA\u6742\u4ea4\u94fe\u8fdb\u884c\u6d88\u5316\uff0c\u6216\u5f15\u8d77DICER\u548cAGO\u4ecb\u5bfc\u7684RNAi\u7cfb\u7edf\u3002mRNA decay\u7684\u5206\u7c7b\u548c\u89e6\u53d1\u6761\u4ef6\u6709\u54ea\u4e9b\u5462\uff1f<\/p>\n\n\n\n<p>[15] Eve AM, Place ES, Smith JC. Comparison of Zebrafish <em>tmem88a<\/em> mutant and morpholino knockdown phenotypes. PLoS One. 2017 Feb 13;12(2):e0172227. doi: 10.1371\/journal.pone.0172227. PMID: 28192479; PMCID: PMC5305201.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u672c\u6587\u63a2\u8ba8\u4e86CRISPR\/Cas9\u57fa\u56e0\u6572\u9664\u4e0eMorpholino\u57fa\u56e0\u6572\u964d\u5728\u53d1\u80b2\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u7684\u8868\u73b0\u5dee\u5f02\u3002\u4f5c\u8005\u68b3\u7406\u4e86\u53cd\u5411\u9057\u4f20\u5b66\u5de5\u5177\u7684\u53d1\u5c55\uff0c\u6307\u51fa\u770b\u4f3c\u66f4\u5f7b\u5e95\u7684\u57fa\u56e0\u6572\u9664\u6709\u65f6\u53cd\u800c\u4ea7\u751f\u6bd4\u6572\u964d\u66f4\u8f7b\u5fae\u7684\u8868\u578b\uff0c\u5e76\u6df1\u5165\u5206\u6790\u4e86\u201c\u8f6c\u5f55\u9002\u5e94\u201d\u7b49\u9057\u4f20\u8865\u507f\u673a\u5236\u53ef\u80fd\u7684\u539f\u56e0\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18,4],"tags":[20],"class_list":["post-143","post","type-post","status-publish","format-standard","hentry","category-18","category-note","tag-crispr"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/143","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=143"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/143\/revisions"}],"predecessor-version":[{"id":14181,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/143\/revisions\/14181"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=143"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=143"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=143"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}