{"id":14317,"date":"2025-12-31T22:35:11","date_gmt":"2025-12-31T14:35:11","guid":{"rendered":"https:\/\/fanyiming.life\/?p=14317"},"modified":"2025-12-31T22:36:54","modified_gmt":"2025-12-31T14:36:54","slug":"2025genomics%e4%bd%9c%e4%b8%9a%e9%a2%98%e9%9b%86%e9%94%a6","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=14317","title":{"rendered":"2025Genomics\u4f5c\u4e1a\u9898\u96c6\u9526"},"content":{"rendered":"\n

\u9898\u76ee1<\/strong><\/h2>\n\n\n\n

\u57fa\u4e8e\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u7684RNA-seq\u5728\u5e26\u6765\u4e30\u5bcc\u7684\u8f6c\u5f55\u7ec4\u6570\u636e\u7684\u540c\u65f6\uff0c\u662f\u5426\u4e5f\u4f1a\u56e0\u5efa\u5e93\u6d41\u7a0b\u3001\u6bd4\u5bf9\/\u5b9a\u91cf\u7b49\u4ea7\u751f\u504f\u5dee\uff1f\u56de\u7b54\uff1a<\/p>\n\n\n\n

1) \u5b83\u53ef\u80fd\u63a9\u76d6\u54ea\u4e9b\u771f\u5b9e\u4fe1\u606f\uff1f<\/p>\n\n\n\n

2) \u5b83\u53ef\u80fd\u5236\u9020\u54ea\u4e9b\u865a\u5047\u4fe1\u53f7\uff1f<\/p>\n\n\n\n

3) \u4ece\u524d\u4e24\u9898\u4efb\u9009\u4e00\u4e2a\u5177\u4f53\u573a\u666f\uff0c\u63d0\u51fa\u4f60\u7684\u4f18\u5316\u65b9\u6848\u3002<\/p>\n\n\n\n

\u53c2\u8003\u7b54\u6848\uff1a<\/strong><\/p>\n\n\n\n

1) \u57fa\u4e8e\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u7684RNA-seq\u53ef\u80fd\u63a9\u76d6\u54ea\u4e9b\u771f\u5b9e\u4fe1\u606f\uff1f<\/strong><\/h3>\n\n\n\n

1. \u4f4e\u4e30\u5ea6\u8f6c\u5f55\u672c\u4e22\u5931\uff1a\u5efa\u5e93\u4e2d\u7684 PCR \u6269\u589e\u504f\u597d\u6027\u53ca\u6d4b\u5e8f\u6df1\u5ea6\u9650\u5236\uff0c\u53ef\u80fd\u5bfc\u81f4\u4f4e\u8868\u8fbe\u57fa\u56e0\u6216\u7a00\u6709\u5f02\u6784\u4f53\u672a\u88ab\u68c0\u6d4b\u5230\u3002<\/p>\n\n\n\n

2. \u8f6c\u5f55\u672c\u7ed3\u6784\u4fe1\u606f\u7f3a\u5931\uff1a\u77ed\u8bfb\u957f\u6d4b\u5e8f\u96be\u4ee5\u51c6\u786e\u89e3\u6790\u5168\u957f\u8f6c\u5f55\u672c\uff0c\u53ef\u80fd\u63a9\u76d6\u53ef\u53d8\u526a\u5207\u3001\u878d\u5408\u57fa\u56e0\u6216 UTR \u533a\u7684\u7cbe\u7ec6\u7ed3\u6784\u3002<\/p>\n\n\n\n

3. \u975e\u7f16\u7801 RNA \u6f0f\u68c0\uff1a\u90e8\u5206\u957f\u94fe\u975e\u7f16\u7801 RNA \u6216\u73af\u5f62 RNA \u56e0\u5efa\u5e93\u65b9\u6cd5\uff08\u5982 rRNA \u53bb\u9664\u3001polyA \u5bcc\u96c6\u7b49\uff09\u800c\u88ab\u6f0f\u68c0\uff1brRNA \u53bb\u9664\u6b65\u9aa4\u53ef\u80fd\u4f1a\u8bef\u6740\u4e00\u4e9b\u4e0e rRNA \u5e8f\u5217\u76f8\u4f3c\u7684\u975e rRNA \u57fa\u56e0\u3002<\/p>\n\n\n\n

4. RNA \u964d\u89e3\u5bfc\u81f4\u7684\u504f\u5dee\uff1a\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\u7684 RNA \u7a33\u5b9a\u6027\u4e0d\u540c\uff0c\u957f\u8f6c\u5f55\u672c\u5728\u63d0\u53d6\u4e2d\u66f4\u6613\u964d\u89e3\u3002\u4f7f\u7528 RNA \u5b8c\u6574\u6027\u503c\u8f83\u4f4e\u7684\u6837\u672c\u5efa\u5e93\u4f1a\u5f15\u5165\u7cfb\u7edf\u6027\u504f\u5dee\uff1b\u5bf9\u4e8e\u90e8\u5206\u964d\u89e3\u6837\u672c\uff0coligo-dT \u6cd5\u4f1a\u5bfc\u81f4\u6d4b\u5e8f\u8bfb\u6570\u4e25\u91cd\u504f\u5411\u4e8e\u57fa\u56e0\u7684 3\u2019\u7aef\u3002<\/p>\n\n\n\n

5. \u7b49\u4f4d\u57fa\u56e0\u7279\u5f02\u6027\u8868\u8fbe\u5206\u6790\u56f0\u96be\uff1a\u964d\u89e3\u53ef\u80fd\u4e0d\u5747\u5300\u5730\u5f71\u54cd\u6765\u81ea\u4e0d\u540c\u7b49\u4f4d\u57fa\u56e0\u7684\u8f6c\u5f55\u672c\uff0c\u5bfc\u81f4\u9519\u8bef\u7684\u7b49\u4f4d\u57fa\u56e0\u504f\u5411\u6027\u7ed3\u8bba\u3002<\/p>\n\n\n\n

6. \u968f\u673a\u5f15\u7269\u504f\u5dee\uff1a\u5728 cDNA \u5408\u6210\u4e2d\uff0c\u968f\u673a\u5f15\u7269\u7684\u7ed3\u5408\u6548\u7387\u5e76\u975e\u5b8c\u5168\u968f\u673a\uff0c\u53ef\u80fd\u53d7\u5230 RNA \u4e8c\u7ea7\u7ed3\u6784\u7684\u5f71\u54cd\uff0c\u5bfc\u81f4\u8f6c\u5f55\u672c\u4e0d\u540c\u533a\u57df\u8986\u76d6\u5ea6\u4e0d\u5747\u5300\u3002<\/p>\n\n\n\n

7. \u591a\u91cd\u6bd4\u5bf9\u95ee\u9898\uff1a\u5bf9\u4e8e\u91cd\u590d\u5e8f\u5217\u6216\u540c\u6e90\u57fa\u56e0\uff0c\u5982\u679c\u4e22\u5f03\u591a\u91cd\u6bd4\u5bf9 reads\uff0c\u8fd9\u4e9b\u57fa\u56e0\u7684\u8868\u8fbe\u91cf\u4f1a\u88ab\u4f4e\u4f30\uff1b\u5982\u679c\u968f\u673a\u5206\u914d\uff0c\u5219\u53ef\u80fd\u5bfc\u81f4\u5b9a\u91cf\u4e0d\u51c6\u786e\u3002<\/p>\n\n\n\n

8. \u53c2\u8003\u57fa\u56e0\u7ec4\u4e0e\u6ce8\u91ca\u7684\u5c40\u9650\uff1a\u53c2\u8003\u57fa\u56e0\u7ec4\u8d28\u91cf\u5f71\u54cd\u6bd4\u5bf9\u6548\u679c\uff0c\u4e14\u6837\u54c1\u5b9e\u9645\u57fa\u56e0\u7ec4\u4e0e\u53c2\u8003\u57fa\u56e0\u7ec4\u5b58\u5728\u5dee\u5f02\uff1b\u57fa\u56e0\u7ec4\u6ce8\u91ca\u6587\u4ef6\u7684\u4e0d\u5b8c\u6574\u6027\u4f1a\u5bfc\u81f4\u65b0\u578b\u8f6c\u5f55\u672c\u548c\u5f02\u6784\u4f53\u56e0\u4e0d\u5b8c\u6574\u7684\u53c2\u8003\u8f6c\u5f55\u7ec4\u800c\u88ab\u63a9\u76d6\u3002<\/p>\n\n\n\n

9. RNA \u8868\u89c2\u4fee\u9970\u4fe1\u606f\u4e22\u5931\uff1a\u9006\u8f6c\u5f55\u7b49\u6b65\u9aa4\u901a\u5e38\u4f1a\u62b9\u9664 RNA \u8868\u89c2\u4fee\u9970\u4fe1\u606f\uff1b\u4e00\u4e9b\u5316\u5b66\u4fee\u9970\uff08\u5982 m\u2076A\u30012′-O-\u7532\u57fa\u5316\uff09\u4f1a\u5f71\u54cd\u63a5\u5934\u8fde\u63a5\u6548\u7387\u6216\u9006\u8f6c\u5f55\u8fc7\u7a0b\uff0c\u4f7f\u5f97\u5e26\u6709\u4fee\u9970\u7684 RNA \u5206\u5b50\u88ab\u9009\u62e9\u6027\u6392\u9664\u3002<\/p>\n\n\n\n

10. \u7247\u6bb5\u5316\u504f\u5dee\uff1a\u7247\u6bb5\u5316\u7684\u975e\u968f\u673a\u6027\u53ef\u80fd\u5f15\u5165\u7cfb\u7edf\u8bef\u5dee\u3002<\/p>\n\n\n\n

11. \u94fe\u7279\u5f02\u6027\u4fe1\u606f\u4e22\u5931\uff1a\u5e38\u89c4\u975e\u94fe\u7279\u5f02\u6027\u5efa\u5e93\u4f1a\u5bfc\u81f4\u8f6c\u5f55\u672c\u6765\u6e90\u65b9\u5411\u4fe1\u606f\u4e22\u5931\uff08\u53ef\u901a\u8fc7\u94fe\u7279\u5f02\u6027\u5efa\u5e93\u8bd5\u5242\u76d2\u907f\u514d\uff09\u3002<\/p>\n\n\n\n

12. RNA \u7f16\u8f91\u4e8b\u4ef6\u88ab\u8bef\u5224\uff1aRNA \u7f16\u8f91\u53ef\u80fd\u88ab\u8bef\u8ba4\u4e3a\u662f\u6d4b\u5e8f\u9519\u8bef\u6216\u6bd4\u5bf9\u9519\u8bef\uff0c\u5c24\u5176\u662f\u5728\u4f7f\u7528\u6807\u51c6\u6bd4\u5bf9\u5de5\u5177\u65f6\uff0c\u8fd9\u4e9b\u540e\u8f6c\u5f55\u4fee\u9970\u4e8b\u4ef6\u53ef\u80fd\u88ab\u8fc7\u6ee4\u6389\u3002<\/p>\n\n\n\n

2) \u57fa\u4e8e\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u7684RNA-seq\u53ef\u80fd\u5236\u9020\u54ea\u4e9b\u865a\u5047\u4fe1\u53f7\uff1f<\/strong><\/h3>\n\n\n\n

1. \u57fa\u56e0\u7ec4DNA\u6c61\u67d3\uff1a\u6837\u672c\u4e2d\u6b8b\u7559\u7684\u57fa\u56e0\u7ec4DNA\u53ef\u80fd\u88ab\u8bef\u8ba4\u4e3a\u662f\u8f6c\u5f55\u672c\u8868\u8fbe\u4fe1\u53f7\uff0c\u5bfc\u81f4\u57fa\u56e0\u8868\u8fbe\u91cf\u7279\u522b\u662f\u4f4e\u8868\u8fbe\u57fa\u56e0\u88ab\u9ad8\u4f30\uff0c\u6216\u9020\u6210\u5185\u542b\u5b50\u4fdd\u7559\u7684\u5047\u8c61\u3002<\/p>\n\n\n\n

2. \u5d4c\u5408\u4f53\u5047\u8c61\uff1a\u5728\u53cd\u8f6c\u5f55\u6216PCR\u6269\u589e\u8fc7\u7a0b\u4e2d\uff0c\u6a21\u677f\u8f6c\u6362\u7b49\u673a\u5236\u53ef\u80fd\u5bfc\u81f4\u4e0d\u76f8\u5173\u7684RNA\u7247\u6bb5\u9519\u8bef\u8fde\u63a5\uff0c\u4ea7\u751f\u865a\u5047\u7684\u878d\u5408\u57fa\u56e0\u6216\u5d4c\u5408\u8f6c\u5f55\u672c\u4fe1\u53f7\u3002<\/p>\n\n\n\n

3. \u5e8f\u5217\u76f8\u4f3c\u6027\u5bfc\u81f4\u7684\u9519\u8bef\u6bd4\u5bf9\uff1a\u9ad8\u5ea6\u540c\u6e90\u7684\u57fa\u56e0\u5bb6\u65cf\u6210\u5458\u6216\u5047\u57fa\u56e0\uff0c\u53ef\u80fd\u56e0\u5e8f\u5217\u76f8\u4f3c\u800c\u88ab\u9519\u8bef\u6bd4\u5bf9\uff0c\u5bfc\u81f4\u5e76\u672a\u8868\u8fbe\u7684\u5047\u57fa\u56e0\u663e\u793a\u51fa\u865a\u5047\u7684\u8868\u8fbe\u4fe1\u53f7\u3002<\/p>\n\n\n\n

4. \u5f15\u5165\u6027\u7a81\u53d8\u4e0e\u5047\u53d8\u5f02\uff1a\u9006\u8f6c\u5f55\u9176\u5728RNA\u4fee\u9970\u4f4d\u70b9\uff08\u5982m6A\uff09\u53ef\u80fd\u53d1\u751f\u78b1\u57fa\u9519\u914d\uff0c\u6216\u5f15\u7269\u975e\u7279\u5f02\u6027\u7ed3\u5408\u5f15\u5165\u672b\u7aef\u7a81\u53d8\uff0c\u8fd9\u4e9b\u6280\u672f\u6027\u9519\u8bef\u53ef\u80fd\u88ab\u8bef\u5224\u4e3aSNV\u6216RNA\u7f16\u8f91\u4e8b\u4ef6\u3002<\/p>\n\n\n\n

5. \u591a\u514b\u9686\u7c07\u6c61\u67d3\uff1a\u5728\u6d4b\u5e8f\u82af\u7247\u4e0a\uff0c\u7a7a\u95f4\u8ddd\u79bb\u8fc7\u8fd1\u7684DNA\u5206\u5b50\u53ef\u80fd\u5f62\u6210\u6df7\u5408\u4fe1\u53f7\u7684\u7c07\uff0c\u5bfc\u81f4\u8367\u5149\u4fe1\u53f7\u8bc6\u522b\u9519\u8bef\uff0c\u4ea7\u751f\u4f4e\u8d28\u91cf\u8bfb\u6bb5\u6216\u9519\u8bef\u7684\u5e8f\u5217\u4fe1\u606f\u3002<\/p>\n\n\n\n

6. \u524d\u4f53RNA\u5e72\u6270\uff1a\u672a\u6210\u719f\u7684pre-mRNA\u4e2d\u7684\u5185\u542b\u5b50\u5e8f\u5217\u82e5\u672a\u88ab\u6709\u6548\u533a\u5206\uff0c\u4f1a\u88ab\u8ba1\u5165\u8868\u8fbe\u91cf\uff0c\u5bfc\u81f4\u5916\u663e\u5b50\u8868\u8fbe\u6c34\u5e73\u88ab\u9ad8\u4f30\u6216\u8bef\u5224\u4e3a\u53ef\u53d8\u526a\u5207\u4e8b\u4ef6\u3002<\/p>\n\n\n\n

7. \u6280\u672f\u504f\u5dee\u5bfc\u81f4\u7684\u901a\u8def\u5bcc\u96c6\u5047\u8c61\uff1a\u5177\u6709\u76f8\u4f3cGC\u542b\u91cf\u6216\u957f\u5ea6\u7279\u5f81\u7684\u57fa\u56e0\u53ef\u80fd\u53d7\u5230\u76f8\u540c\u7684\u6280\u672f\u504f\u5dee\u5f71\u54cd\uff0c\u5bfc\u81f4\u8fd9\u4e9b\u57fa\u56e0\u5728\u7279\u5b9a\u901a\u8def\u4e2d\u8868\u73b0\u51fa\u865a\u5047\u7684\u534f\u540c\u53d8\u5316\u3002<\/p>\n\n\n\n

8. \u63a5\u5934\u6c61\u67d3\uff1a\u6587\u5e93\u6784\u5efa\u4e2d\u7684\u63a5\u5934\u5e8f\u5217\u82e5\u672a\u88ab\u5f7b\u5e95\u53bb\u9664\uff0c\u53ef\u80fd\u5bfc\u81f4\u6bd4\u5bf9\u9519\u8bef\u6216\u4ea7\u751f\u865a\u5047\u7684\u975e\u57fa\u56e0\u7ec4\u5e8f\u5217\u4fe1\u53f7\u3002<\/p>\n\n\n\n

3) \u4f18\u5316\u65b9\u6cd5\uff1a<\/strong><\/h3>\n\n\n\n

1. \u5728\u5efa\u5e93\u8fc7\u7a0b\u4e2d\u5f15\u5165\u5206\u5b50\u6761\u5f62\u7801\uff08UMI\uff09\uff0c\u6709\u6548\u533a\u5206PCR\u91cd\u590d\uff0c\u6d88\u9664\u6269\u589e\u504f\u597d\u6027\u5bf9\u5b9a\u91cf\u7684\u5f71\u54cd\u3002<\/p>\n\n\n\n

2. \u91c7\u7528\u4e09\u4ee3\u957f\u8bfb\u957f\u6d4b\u5e8f\u6280\u672f\uff0c\u76f4\u63a5\u83b7\u53d6\u5168\u957f\u8f6c\u5f55\u672c\uff0c\u5f25\u8865\u77ed\u8bfb\u957f\u6d4b\u5e8f\u5728\u7ed3\u6784\u89e3\u6790\u4e0a\u7684\u4e0d\u8db3\u3002<\/p>\n\n\n\n

3. \u4e25\u683c\u8fdb\u884cDNase I\u5904\u7406\uff0c\u5f7b\u5e95\u53bb\u9664\u57fa\u56e0\u7ec4DNA\u6c61\u67d3\uff0c\u907f\u514d\u5176\u4ea7\u751f\u7684\u865a\u5047\u8f6c\u5f55\u4fe1\u53f7\u3002<\/p>\n\n\n\n

4. \u6dfb\u52a0Spike-in\u5916\u90e8\u6807\u51c6\u54c1\uff0c\u7528\u4e8e\u6821\u6b63\u6279\u6b21\u6548\u5e94\uff0c\u76d1\u63a7\u5b9e\u9a8c\u95f4\u7684\u7cfb\u7edf\u6027\u8bef\u5dee\u3002<\/p>\n\n\n\n

5. \u6784\u5efa\u4e2a\u6027\u5316\u53c2\u8003\u57fa\u56e0\u7ec4\uff0c\u51cf\u5c11\u56e0\u53c2\u8003\u5e8f\u5217\u4e0e\u6837\u672c\u57fa\u56e0\u7ec4\u5dee\u5f02\u5bfc\u81f4\u7684\u6bd4\u5bf9\u504f\u5dee\u3002<\/p>\n\n\n\n

6. \u4f7f\u7528Salmon\u6216Kallisto\u7b49\u4e13\u95e8\u5904\u7406\u591a\u91cd\u6bd4\u5bf9\u7684\u5de5\u5177\uff0c\u901a\u8fc7\u6982\u7387\u6a21\u578b\u66f4\u51c6\u786e\u5730\u5b9a\u91cf\u91cd\u590d\u5e8f\u5217\u533a\u57df\u3002<\/p>\n\n\n\n

7. \u5229\u7528qPCR\u7b49\u5b9e\u9a8c\u624b\u6bb5\u5bf9\u5173\u952e\u53d1\u73b0\u8fdb\u884c\u9a8c\u8bc1\uff0c\u6392\u9664\u6d4b\u5e8f\u6216\u5206\u6790\u5f15\u5165\u7684\u5047\u9633\u6027\u7ed3\u679c\u3002<\/p>\n\n\n\n

—<\/p>\n\n\n\n

\u9898\u76ee2<\/strong><\/h2>\n\n\n\n

\u73b0\u4ee3\u6570\u636e\u5e93\u4e2d\u7684\u7edd\u5927\u591a\u6570transcript variants\u90fd\u6765\u81eaRNA-Seq\u53ca\u5176\u884d\u751f\u6280\u672f\u3002\u7136\u800c\uff0c\u5bf9GU-AG motif\u7b49\u7684\u4f9d\u8d56\uff0c\u4e5f\u8bb8\u8ba9\u6211\u4eec\u5ffd\u89c6\u4e86\u5bf9”\u7ecf\u5178\u4ee5\u5916”\u7684\u526a\u63a5\u4e8b\u4ef6\u7684\u53d1\u73b0\u3002\u56de\u7b54\uff1a<\/p>\n\n\n\n

1) \u8c03\u67e5XBP1s\u5728\u5185\u8d28\u7f51\u5e94\u6fc0\u4e2d\u7684\u975e\u5e38\u89c4\u526a\u63a5\u6d41\u7a0b\uff1a\u5207\u5272\u9176\u3001\u65ad\u7aef\u5316\u5b66\u6027\u8d28\u3001\u8fde\u63a5\u9176\uff0c\u6307\u51fa\u5176\u5728\u5e38\u89c4RNA-seq\u6d41\u7a0b\u4e2d\u4e3a\u4f55\u4e0d\u6613\u88ab\u6355\u83b7\u3002<\/p>\n\n\n\n

2) \u5728\u4e0d\u6539\u53d8\u5b9e\u9a8c\u6570\u636e\u7684\u524d\u63d0\u4e0b\uff0c\u63d0\u51fa\u4e00\u6761\u4f18\u5316\u7684\u6570\u636e\u5206\u6790\u6d41\u7a0b\u4ee5\u53d1\u73b0\u66f4\u591aXBP1s\u540c\u7c7b\u4e8b\u4ef6\uff08\u975e\u5e38\u89c4\u5207\u5272 + \u8fde\u63a5\uff09\u3002<\/p>\n\n\n\n

\u53c2\u8003\u7b54\u6848\uff1a<\/strong><\/p>\n\n\n\n

1) XBP1s \u5728\u5e38\u89c4 RNA-seq \u6d41\u7a0b\u4e2d\u4e0d\u6613\u88ab\u6355\u83b7\u7684\u539f\u56e0\u5206\u6790<\/strong><\/h3>\n\n\n\n

1. \u65ad\u7aef\u5316\u5b66\u6027\u8d28\u7279\u6b8a\uff1aIRE1\u03b1 \u526a\u5207\u4ea7\u751f 2′,3′-\u73af\u78f7\u9178\u672b\u7aef\uff0c\u4f1a\u963b\u788d\u5e38\u89c4\u5efa\u5e93\u4e2d\u7684\u63a5\u5934\u8fde\u63a5\uff0c\u82e5\u4e0d\u8fdb\u884c\u989d\u5916\u7684\u53bb\u78f7\u9178\u5316\u5904\u7406\uff0cXBP1s \u5e8f\u5217\u96be\u4ee5\u8fdb\u5165\u6d4b\u5e8f\u6587\u5e93\u3002<\/p>\n\n\n\n

2. \u526a\u63a5\u4f4d\u70b9\u7279\u5f81\u975e\u5e38\u89c4\uff1aXBP1 \u7684\u526a\u63a5\u4e0d\u9075\u5faa\u7ecf\u5178\u526a\u63a5\u4f53\u7684 GU-AG \u89c4\u5f8b\uff0c\u800c\u5e38\u89c4\u5206\u6790\u6d41\u7a0b\u4e2d\u7684\u526a\u63a5\u4f4d\u70b9\u8bc6\u522b\u6a21\u5757\u901a\u5e38\u4f9d\u8d56\u4e8e\u8fd9\u4e9b\u4fdd\u5b88\u7684 motif \u4fe1\u53f7\u3002<\/p>\n\n\n\n

3. \u5185\u542b\u5b50\u957f\u5ea6\u6781\u77ed\uff1aXBP1s \u526a\u63a5\u53bb\u9664\u7684\u7247\u6bb5\u4ec5\u4e3a 26 nt\uff0c\u663e\u8457\u77ed\u4e8e\u5e38\u89c4\u5185\u542b\u5b50\u7684\u957f\u5ea6\u8303\u56f4\uff0c\u5e38\u88ab\u5206\u6790\u8f6f\u4ef6\u7684\u8fc7\u6ee4\u7b97\u6cd5\u89c6\u4f5c\u6bd4\u5bf9\u9519\u8bef\u6216\u566a\u58f0\u800c\u5254\u9664\u3002<\/p>\n\n\n\n

4. \u6570\u636e\u5e93\u6ce8\u91ca\u4e0d\u5b8c\u6574\uff1a\u73b0\u6709\u7684\u8f6c\u5f55\u7ec4\u6ce8\u91ca\u6587\u4ef6\u53ef\u80fd\u672a\u6db5\u76d6\u6b64\u7c7b\u975e\u5e38\u89c4\u526a\u63a5\u4e8b\u4ef6\uff0c\u5bfc\u81f4\u8de8\u8d8a\u526a\u63a5\u4f4d\u70b9\u7684 reads \u5728\u5b9a\u91cf\u9636\u6bb5\u88ab\u8bc6\u522b\u4e3a\u672a\u6ce8\u91ca\u5e8f\u5217\u6216\u57fa\u56e0\u95f4\u533a\u566a\u58f0\u3002<\/p>\n\n\n\n

5. \u751f\u7269\u5b66\u53d1\u751f\u6761\u4ef6\u53d7\u9650\uff1a\u8be5\u526a\u63a5\u4e8b\u4ef6\u7279\u5f02\u6027\u5730\u53d1\u751f\u4e8e\u5185\u8d28\u7f51\u5e94\u6fc0\u72b6\u6001\u4e0b\uff0c\u82e5\u5b9e\u9a8c\u8bf1\u5bfc\u4e0d\u8db3\u6216\u91c7\u6837\u65f6\u95f4\u70b9\u504f\u79bb\u5e94\u6fc0\u9ad8\u5cf0\uff0c\u5176\u8f6c\u5f55\u672c\u4e30\u5ea6\u53ef\u80fd\u4f4e\u4e8e\u68c0\u6d4b\u4e0b\u9650\u3002<\/p>\n\n\n\n

2) \u5728\u4e0d\u6539\u53d8\u5b9e\u9a8c\u6570\u636e\u7684\u524d\u63d0\u4e0b\uff0c \u4f18\u5316\u6570\u636e\u5206\u6790\u6d41\u7a0b\u4ee5\u53d1\u73b0\u66f4\u591aXBP1s\u540c\u7c7b\u4e8b\u4ef6<\/strong><\/h3>\n\n\n\n

1. \u8c03\u6574\u6bd4\u5bf9\u7b97\u6cd5\u53c2\u6570\uff0c\u663e\u8457\u964d\u4f4e\u6700\u5c0f\u5185\u542b\u5b50\u957f\u5ea6\u9608\u503c\uff08\u5982\u8bbe\u5b9a\u4e3a 10 nt\uff09\uff0c\u5e76\u5bf9\u672a\u6210\u529f\u6bd4\u5bf9\u7684 reads \u8fdb\u884c\u91cd\u65b0\u626b\u63cf\uff0c\u4ee5\u6355\u83b7\u6781\u77ed\u7684\u526a\u63a5\u7247\u6bb5\u3002<\/p>\n\n\n\n

2. \u91c7\u7528\u4e0d\u4f9d\u8d56\u73b0\u6709\u6ce8\u91ca\u6587\u4ef6\u7684\u4ece\u5934\u7ec4\u88c5\u7b56\u7565\uff0c\u901a\u8fc7\u63d0\u53d6\u65b0\u526a\u63a5\u4f4d\u70b9\u4fe1\u606f\uff0c\u8bc6\u522b\u6570\u636e\u5e93\u4e2d\u672a\u6536\u5f55\u7684\u975e\u5e38\u89c4\u8f6c\u5f55\u672c\u3002<\/p>\n\n\n\n

3. \u501f\u9274\u73af\u72b6 RNA \u6216\u878d\u5408\u57fa\u56e0\u7684\u5206\u6790\u601d\u8def\uff0c\u5229\u7528\u5176\u68c0\u6d4b\u975e\u5171\u7ebf\u6027\u8fde\u63a5\u7684\u7b97\u6cd5\uff0c\u5bfb\u627e\u57fa\u56e0\u7ec4\u77ed\u8ddd\u79bb\u5185\u7684\u5f02\u5e38\u8fde\u63a5\u4e8b\u4ef6\u3002<\/p>\n\n\n\n

4. \u8fc7\u6ee4\u6389\u7b26\u5408 GU-AG \u7b49\u7ecf\u5178\u526a\u63a5\u4fe1\u53f7\u7684\u5e8f\u5217\uff0c\u901a\u8fc7\u6392\u9664\u6cd5\u91cd\u70b9\u9501\u5b9a\u975e\u4fdd\u5b88\u7684\u526a\u63a5\u4f4d\u70b9\u3002<\/p>\n\n\n\n

5. \u7ed3\u5408 RNA \u4e8c\u7ea7\u7ed3\u6784\u9884\u6d4b\u5de5\u5177\uff0c\u7b5b\u9009\u5728\u526a\u63a5\u4f4d\u70b9\u4e0a\u4e0b\u6e38\u5177\u6709\u7279\u5b9a\u201c\u830e\u73af\u201d\u7ed3\u6784\u7684\u5019\u9009\u5e8f\u5217\uff0c\u5339\u914d IRE1\u03b1 \u7684\u4f5c\u7528\u7279\u5f81\u3002<\/p>\n\n\n\n

6. \u4f7f\u7528\u57fa\u5e8f\u5206\u6790\u5de5\u5177\u63d0\u53d6\u5019\u9009\u4f4d\u70b9\u7684\u5e8f\u5217\u7279\u5f81\uff0c\u9a8c\u8bc1\u5176\u662f\u5426\u5bcc\u96c6\u4e86\u7c7b\u4f3c\u4e8e IRE1\u03b1 \u7b49\u6838\u9178\u9176\u7684\u4fdd\u5b88\u8bc6\u522b motif\u3002<\/p>\n\n\n\n

7. \u5f15\u5165\u751f\u7269\u5b66\u80cc\u666f\u9a8c\u8bc1\uff0c\u5bf9\u6bd4\u4e0d\u540c\u5b9e\u9a8c\u7ec4\u6570\u636e\uff0c\u786e\u8ba4\u5019\u9009\u526a\u63a5\u4e8b\u4ef6\u662f\u5426\u4ec5\u5728\u7279\u5b9a\u7684\u751f\u7269\u5b66\u5e94\u6fc0\u72b6\u6001\u4e0b\u7279\u5f02\u6027\u53d1\u751f\u3002<\/p>\n\n\n\n

—<\/p>\n\n\n\n

\u9898\u76ee3<\/strong><\/h2>\n\n\n\n

Transposon\u4f5c\u4e3a\u4e00\u7c7b\u9ad8\u5ea6\u91cd\u590d\u7684DNA element\uff0c\u5728\u57fa\u4e8e\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u7684RNA-seq\u7684\u6570\u636e\u5206\u6790\u4e2d\uff0c\u5e38\u9762\u4e34\u591a\u91cd\u6bd4\u5bf9\u3001\u4e0e\u5176\u4ed6\u57fa\u56e0locus\u91cd\u5408\u7b49\u95ee\u9898\u3002\u56de\u7b54\uff1a<\/p>\n\n\n\n

1) \u4ece\u5efa\u5e93\u6d41\u7a0b\u4e0e\u7b97\u6cd5\u5047\u8bbe\u4e24\u4e2a\u5c42\u9762\u5206\u6790\uff0c\u4e3a\u4ec0\u4e48\u8f6c\u5ea7\u5b50\u8f6c\u5f55\u672c\u5728\u57fa\u4e8e\u4e8c\u4ee3\u6d4b\u5e8f\u7684RNA-seq\u6570\u636e\u4e2d\u96be\u4ee5\u88ab\u51c6\u786e\u8bc6\u522b\u4e0e\u5b9a\u91cf\uff1f<\/p>\n\n\n\n

2) \u5982\u4f55\u5728transposon\u7684\u56fa\u6709\u9650\u5236\u4e0b\uff0c\u4f9d\u7136\u4eceRNA-seq\u4e2d\u66f4\u6709\u6548\u5730\u6355\u6349\u5230\u8f6c\u5ea7\u5b50\u76f8\u5173\u7684\u8f6c\u5f55\u4e8b\u4ef6\uff1f<\/p>\n\n\n\n

\u53c2\u8003\u7b54\u6848\uff1a<\/strong><\/p>\n\n\n\n

1) \u4ece\u5efa\u5e93\u6d41\u7a0b\u4e0e\u7b97\u6cd5\u5047\u8bbe\u4e24\u4e2a\u5c42\u9762\u5206\u6790\uff0c\u4e3a\u4ec0\u4e48\u8f6c\u5ea7\u5b50\u8f6c\u5f55\u672c\u5728\u57fa\u4e8e\u4e8c\u4ee3\u6d4b\u5e8f\u7684RNA-seq\u6570\u636e\u4e2d\u96be\u4ee5\u88ab\u51c6\u786e\u8bc6\u522b\u4e0e\u5b9a\u91cf\uff1f<\/strong><\/h3>\n\n\n\n

1. \u90e8\u5206\u8f6c\u5ea7\u5b50\u8f6c\u5f55\u672c\u56e0\u7f3a\u4e4fpolyA\u5c3e\u6216\u5177\u6709\u7c7brRNA\u5e8f\u5217\u7279\u5f81\uff0c\u5728\u5e38\u89c4\u5efa\u5e93\u5bcc\u96c6\u73af\u8282\u6613\u88ab\u7cfb\u7edf\u6027\u6392\u9664\u3002<\/p>\n\n\n\n

2. \u8f6c\u5ea7\u5b50\u5e8f\u5217\uff08\u5982\u9006\u8f6c\u5f55\u8f6c\u5ea7\u5b50\uff09\u4e24\u7aef\u5e38\u5f62\u6210\u7a33\u5b9a\u7684\u53d1\u5361\u7b49\u4e8c\u7ea7\u7ed3\u6784\uff0c\u4f1a\u7269\u7406\u6027\u963b\u788d\u9006\u8f6c\u5f55\u9176\u7684\u8fdb\u7a0b\uff0c\u5f71\u54cd\u6587\u5e93\u6784\u5efa\u6548\u7387\u3002<\/p>\n\n\n\n

3. \u8f6c\u5ea7\u5b50\u5728\u57fa\u56e0\u7ec4\u4e2d\u62f7\u8d1d\u6570\u6781\u9ad8\uff0c\u5efa\u5e93\u65f6\u82e5gDNA\u53bb\u9664\u4e0d\u5f7b\u5e95\uff0c\u6781\u6613\u5f15\u5165\u4e25\u91cd\u7684\u57fa\u56e0\u7ec4DNA\u6c61\u67d3\u4fe1\u53f7\uff0c\u5e72\u6270\u771f\u5b9e\u8f6c\u5f55\u6c34\u5e73\u7684\u5b9a\u91cf\u3002<\/p>\n\n\n\n

4. \u7b97\u6cd5\u6d41\u7a0b\u901a\u5e38\u5047\u8bbereads\u5e94\u5177\u6709\u552f\u4e00\u6bd4\u5bf9\u4f4d\u7f6e\uff0c\u800c\u8f6c\u5ea7\u5b50\u7684\u9ad8\u5ea6\u91cd\u590d\u6027\u5bfc\u81f4\u5176MAPQ\u5206\u6570\u6781\u4f4e\uff0c\u5e38\u88ab\u4e0b\u6e38\u5206\u6790\u8f6f\u4ef6\u4f5c\u4e3a\u4e0d\u53ef\u9760\u6570\u636e\u8fc7\u6ee4\u6389\u3002<\/p>\n\n\n\n

5. \u8f6c\u5ea7\u5b50\u5bcc\u96c6\u7684\u5f02\u67d3\u8272\u8d28\u533a\u57df\u5728\u73b0\u6709\u53c2\u8003\u57fa\u56e0\u7ec4\u7ec4\u88c5\u4e2d\u5f80\u5f80\u5b58\u5728\u5927\u91cf\u7f3a\u53e3\u6216\u672a\u89e3\u6790\u533a\u57df\uff0c\u5bfc\u81f4\u76f8\u5173reads\u65e0\u6cd5\u51c6\u786e\u56de\u5e16\u3002<\/p>\n\n\n\n

6. \u8f6c\u5ea7\u5b50\u8f6c\u5f55\u5e38\u4e0e\u5bbf\u4e3b\u57fa\u56e0\u53d1\u751f\u91cd\u53e0\u6216\u8868\u73b0\u4e3a\u53cd\u4e49\u8f6c\u5f55\uff0c\u5728\u5206\u6790\u5c42\u9762\u96be\u4ee5\u51c6\u786e\u533a\u5206\u5176\u6765\u6e90\u4e8e\u72ec\u7acb\u7684\u8f6c\u5ea7\u5b50\u6d3b\u52a8\u8fd8\u662f\u5bbf\u4e3b\u57fa\u56e0\u7684\u80cc\u666f\u8f6c\u5f55\u3002<\/p>\n\n\n\n

2) \u5982\u4f55\u5728transposon\u7684\u56fa\u6709\u9650\u5236\u4e0b\uff0c\u4f9d\u7136\u4eceRNA seq\u4e2d\u66f4\u6709\u6548\u5730\u6355\u6349\u5230\u8f6c\u5ea7\u5b50\u76f8\u5173\u7684\u8f6c\u5f55\u4e8b\u4ef6\uff1f<\/strong><\/h3>\n\n\n\n

1. \u91c7\u7528rRNA\u53bb\u9664\u6cd5\u800c\u975epolyA\u5bcc\u96c6\uff0c\u4ee5\u4fdd\u7559\u7f3a\u4e4fpolyA\u5c3e\u7684\u8f6c\u5ea7\u5b50\u8f6c\u5f55\u672c\u3002<\/p>\n\n\n\n

2. \u4f7f\u7528\u94fe\u7279\u5f02\u6027\u5efa\u5e93\u6280\u672f\uff0c\u8f85\u52a9\u533a\u5206\u5bbf\u4e3b\u57fa\u56e0\u91cd\u53e0\u533a\u57df\u4e0e\u53cd\u4e49\u8f6c\u5ea7\u5b50\u8f6c\u5f55\u3002<\/p>\n\n\n\n

3. \u91c7\u7528\u914d\u5bf9\u672b\u7aef\u6d4b\u5e8f\uff0c\u901a\u8fc7\u589e\u52a0\u7247\u6bb5\u7684\u552f\u4e00\u6027\u7279\u5f81\u6765\u63d0\u9ad8\u91cd\u590d\u533a\u57df\u7684\u6bd4\u5bf9\u7387\u3002<\/p>\n\n\n\n

4. \u8c03\u6574\u6587\u5e93\u6784\u5efa\u4e2d\u7684\u957f\u5ea6\u7b5b\u9009\u8303\u56f4\uff0c\u4fdd\u7559\u5982Alu\u7b49\u77ed\u7247\u6bb5\u8f6c\u5ea7\u5b50\u5e8f\u5217\u3002<\/p>\n\n\n\n

5. \u9488\u5bf9\u7279\u5b9a\u6d3b\u8dc3\u5bb6\u65cf\u8bbe\u8ba1\u6355\u83b7\u63a2\u9488\u8fdb\u884c\u9776\u5411\u6d4b\u5e8f\uff0c\u63d0\u9ad8\u68c0\u6d4b\u6df1\u5ea6\u4e0e\u7279\u5f02\u6027\u3002<\/p>\n\n\n\n

6. \u7ed3\u5408\u957f\u8bfb\u957f\u6d4b\u5e8f\u6280\u672f\u83b7\u53d6\u5168\u957f\u8f6c\u5f55\u672c\uff0c\u76f4\u63a5\u89e3\u51b3\u91cd\u590d\u5e8f\u5217\u7684\u6bd4\u5bf9\u6a21\u7cca\u6027\u3002<\/p>\n\n\n\n

7. \u4f7f\u7528\u5305\u542b\u5b8c\u6574\u8f6c\u5ea7\u5b50\u5143\u4ef6\u6ce8\u91ca\u7684\u53c2\u8003\u57fa\u56e0\u7ec4\u8fdb\u884c\u6bd4\u5bf9\u3002<\/p>\n\n\n\n

8. \u4fdd\u7559\u591a\u91cd\u6bd4\u5bf9\u8bfb\u6bb5\uff0c\u5e76\u5229\u7528EM\u7b97\u6cd5\u7b49\u6982\u7387\u6a21\u578b\u8fdb\u884c\u5b9a\u91cf\u5206\u914d\u3002<\/p>\n\n\n\n

9. \u5728\u5bb6\u65cf\u6216\u4e9a\u5bb6\u65cf\u6c34\u5e73\u8fdb\u884c\u5b9a\u91cf\u5206\u6790\uff0c\u964d\u4f4e\u5355\u4e00\u4f4d\u70b9\u6bd4\u5bf9\u4e0d\u786e\u5b9a\u6027\u7684\u5f71\u54cd\u3002<\/p>\n\n\n\n

10. \u6bd4\u8f83\u8f6c\u5ea7\u5b50\u5185\u90e8\u4e0e\u4fa7\u7ffc\u533a\u57df\u7684\u8986\u76d6\u6a21\u5f0f\uff0c\u7ed3\u5408\u8f6c\u5f55\u8d77\u59cb\u4f4d\u70b9\u7279\u5f81\u63a8\u65ad\u5176\u8f6c\u5f55\u72ec\u7acb\u6027\u3002<\/p>\n\n\n\n

11. \u5bf9\u9884\u6d4b\u7684\u9ad8\u8868\u8fbe\u6216\u5dee\u5f02\u4f4d\u70b9\u8bbe\u8ba1\u7279\u5f02\u6027\u5f15\u7269\uff0c\u901a\u8fc7RT-PCR\u53caSanger\u6d4b\u5e8f\u8fdb\u884c\u9a8c\u8bc1\u3002<\/p>\n\n\n\n

—<\/p>\n\n\n\n

\u9898\u76ee4\uff08\u5fc5\u9009\uff09<\/strong><\/h2>\n\n\n\n

\u80cc\u666f\u56fe\u7247\u662fIGV (Integrative Genomics Viewer)\u4e2d\u4e00\u5957K562\u7ec6\u80de\uff08DMSO\u5bf9\u7167\u5904\u7406\uff09\u7684RNA-seq\u6570\u636e\u5728\u57fa\u56e0X\u533a\u57df\u9644\u8fd1\u7684\u6bd4\u5bf9\u56fe\u50cf\uff08squished view and collapsed view\uff09\uff0c\u8bf7\u6839\u636e\u56fe\u7247\u4fe1\u606f\u56de\u7b54\u4ee5\u4e0b\u95ee\u9898\uff1a<\/p>\n\n\n\n

1) \u56fe\u4e2d\u53f3\u4fa7\u7684\u6d4b\u5e8freads\u5bc6\u5ea6\u663e\u8457\u9ad8\u4e8e\u5de6\u4fa7\uff0c\u6709\u54ea\u4e9b\u53ef\u80fd\u7684\u539f\u56e0\uff1f\uff08\u81f3\u5c11\u63d0\u51fa\u4e00\u4e2a\u751f\u7269\u5b66\u539f\u56e0\uff0c\u4e00\u4e2a\u6280\u672f\u6027\u539f\u56e0\uff09<\/p>\n\n\n\n

2) \u4ece\u4e0a\u4e00\u95ee\u4e2d\u6311\u9009\u4e00\u4e2a\u751f\u7269\u5b66\u539f\u56e0\u548c\u4e00\u4e2a\u6280\u672f\u6027\u539f\u56e0\uff0c\u63d0\u793a\u9a8c\u8bc1\u5047\u8bbe\u7684\u5b9e\u9a8c\u6216\u5206\u6790\u65b9\u6cd5<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u53c2\u8003\u7b54\u6848\uff1a<\/strong><\/p>\n\n\n\n

1) \u56fe\u4e2d\u53f3\u4fa7\u7684\u6d4b\u5e8freads\u5bc6\u5ea6\u663e\u8457\u9ad8\u4e8e\u5de6\u4fa7\uff0c\u6709\u54ea\u4e9b\u53ef\u80fd\u7684\u539f\u56e0\uff1f<\/strong><\/h3>\n\n\n\n

\u751f\u7269\u5b66\u539f\u56e0\uff1a<\/strong><\/p>\n\n\n\n

1. \u53ef\u53d8\u526a\u63a5\uff1a\u5de6\u4fa7\u533a\u57df\u53ef\u80fd\u5bf9\u5e94\u53ef\u9009\u5916\u663e\u5b50\uff0c\u5728\u4e3b\u8981\u8f6c\u5f55\u672c\u4e2d\u88ab\u8df3\u8fc7\uff0c\u5bfc\u81f4\u5176\u8986\u76d6\u5ea6\u663e\u8457\u4f4e\u4e8e\u5b58\u5728\u4e8e\u6240\u6709\u5f02\u6784\u4f53\u4e2d\u7684\u53f3\u4fa7\u533a\u57df\u3002<\/p>\n\n\n\n

2. \u53ef\u53d8\u8f6c\u5f55\u8d77\u59cb\u4f4d\u70b9\uff1a\u57fa\u56e0\u5b58\u5728\u5185\u90e8\u542f\u52a8\u5b50\uff0c\u5bfc\u81f4\u8f6c\u5f55\u4ece\u5de6\u4fa7\u533a\u57df\u4e0b\u6e38\u5f00\u59cb\uff0c\u4f7f\u5f97\u5de6\u4fa7\u5e8f\u5217\u672a\u88ab\u5305\u542b\u5728\u6210\u719f\u8f6c\u5f55\u672c\u4e2d\u3002<\/p>\n\n\n\n

3. \u67d3\u8272\u4f53\u6613\u4f4d\uff1a\u5728\u7279\u5b9a\u7ec6\u80de\u7cfb\uff08\u5982K562\uff09\u4e2d\uff0c\u53f3\u4fa7\u533a\u57df\u53ef\u80fd\u56e0\u67d3\u8272\u4f53\u6613\u4f4d\uff08\u5982\u5f62\u6210BCR-ABL\u878d\u5408\u57fa\u56e0\uff09\u800c\u53d7\u5230\u5f3a\u542f\u52a8\u5b50\u9a71\u52a8\uff0c\u5bfc\u81f4\u5176\u8868\u8fbe\u6c34\u5e73\u5267\u589e\u3002<\/p>\n\n\n\n

4. \u57fa\u56e0\u91cd\u53e0\uff1a\u53f3\u4fa7\u9ad8\u5bc6\u5ea6\u533a\u53ef\u80fd\u91cd\u53e0\u4e86\u53e6\u4e00\u4e2a\u76f8\u90bb\u7684\u9ad8\u8868\u8fbe\u57fa\u56e0\uff0c\u5bfc\u81f4\u8be5\u4f4d\u7f6e\u7684reads\u5b9e\u9645\u4e0a\u6765\u6e90\u4e8e\u4e24\u4e2a\u4e0d\u540c\u7684\u8f6c\u5f55\u5355\u5143\u3002<\/p>\n\n\n\n

\u6280\u672f\u6027\u539f\u56e0\uff1a<\/strong><\/p>\n\n\n\n

1. 3\u7aef\u504f\u597d\u6027\uff1a\u91c7\u7528polyA\u5bcc\u96c6\u6cd5\u5efa\u5e93\u65f6\uff0c\u7531\u4e8e\u53cd\u8f6c\u5f55\u4ece3\u7aef\u5f00\u59cb\uff0c\u5bb9\u6613\u5bfc\u81f4\u57fa\u56e05\u7aef\uff08\u5de6\u4fa7\uff09\u5e8f\u5217\u7684\u6355\u83b7\u6548\u7387\u964d\u4f4e\u3002<\/p>\n\n\n\n

2. \u6837\u672c\u964d\u89e3\uff1aRNA\u964d\u89e3\u901a\u5e38\u4ece\u4e0d\u5e26\u4fdd\u62a4\u76845\u7aef\u5f00\u59cb\uff0c\u5bfc\u81f4\u5efa\u5e93\u65f6\u8f6c\u5f55\u672c\u5de6\u4fa7\u7247\u6bb5\u4e22\u5931\uff0c\u53f3\u4fa7\u76f8\u5bf9\u5b8c\u6574\u3002<\/p>\n\n\n\n

3. \u6bd4\u5bf9\u8fc7\u6ee4\uff1a\u5de6\u4fa7\u533a\u57df\u53ef\u80fd\u5305\u542b\u91cd\u590d\u5e8f\u5217\u6216\u4f4e\u590d\u6742\u5ea6\u5e8f\u5217\uff0c\u5bfc\u81f4\u76f8\u5173reads\u56e0\u591a\u91cd\u6bd4\u5bf9\u6216\u4f4e\u6bd4\u5bf9\u8d28\u91cf\u88ab\u8f6f\u4ef6\u8fc7\u6ee4\u3002<\/p>\n\n\n\n

4. \u6269\u589e\u504f\u5dee\uff1a\u53f3\u4fa7\u5e8f\u5217\u7684GC\u542b\u91cf\u6216\u4e8c\u7ea7\u7ed3\u6784\u53ef\u80fd\u66f4\u9002\u5408PCR\u6269\u589e\uff0c\u5bfc\u81f4\u5176\u5728\u6587\u5e93\u6784\u5efa\u8fc7\u7a0b\u4e2d\u88ab\u8fc7\u5ea6\u5bcc\u96c6\u3002<\/p>\n\n\n\n

5. \u6ce8\u91ca\u7f3a\u5931\uff1a\u53f3\u4fa7\u533a\u57df\u53ef\u80fd\u5b58\u5728\u672a\u88ab\u8bb0\u5f55\u7684\u72ec\u7acb\u9ad8\u8868\u8fbe\u8f6c\u5f55\u672c\u6216\u975e\u7f16\u7801RNA\uff0c\u56e0\u7f3a\u4e4f\u6ce8\u91ca\u800c\u88ab\u8bef\u8ba4\u4e3a\u5c5e\u4e8e\u8be5\u57fa\u56e0\u3002<\/p>\n\n\n\n

6. \u89c6\u56fe\u6e32\u67d3\uff1aIGV\u7b49\u53ef\u89c6\u5316\u5de5\u5177\u5728\u4e0d\u540c\u89c6\u56fe\u6a21\u5f0f\uff08\u5982Squished View\uff09\u4e0b\u7684\u7f29\u653e\u4e0e\u5e73\u6ed1\u7b97\u6cd5\u53ef\u80fd\u5728\u89c6\u89c9\u4e0a\u5938\u5927\u4e86\u5c40\u90e8\u7684\u8986\u76d6\u5ea6\u5dee\u5f02\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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