{"id":1600,"date":"2024-03-29T19:22:53","date_gmt":"2024-03-29T11:22:53","guid":{"rendered":"https:\/\/fanyiming.life\/?p=1600"},"modified":"2025-12-21T01:05:59","modified_gmt":"2025-12-20T17:05:59","slug":"%e7%bb%86%e8%83%9e%e8%bd%ac%e6%9f%93%e7%bb%86%e8%8a%82","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=1600","title":{"rendered":"\u7ec6\u80de\u8f6c\u67d3\u7ec6\u8282"},"content":{"rendered":"\n<ol class=\"wp-block-list\">\n<li>\u8f6c\u67d3\u524d\u6362\u6db2\u548c\u63a7\u5236confluence\u572860\uff5e70%\u5bf9\u63d0\u9ad8\u8f6c\u67d3\u6548\u7387\u5f88\u91cd\u8981\u3002<\/li>\n\n\n\n<li>\u8f6c\u67d3\u540e48h\u8868\u8fbe\u6700\u9ad8\u3002\u4e00\u4e2a\u4f8b\u5b50\u662f\u8f6c\u67d3\u540e21.5h\u8367\u5149\u9633\u6027\u738711%\uff0c\u8f6c\u67d3\u540e48h\u53d8\u621034%\u4e86\u3002<\/li>\n\n\n\n<li>\u5047\u5982\u4f60\u540c\u65f6\u8f6c\u67d33\u4e2a\u8d28\u7c92\uff0c\u5bf9\u4e8e\u67d0\u4e2a\u7ec6\u80de\u6765\u8bf4\uff0c\u80fd\u8f6c\u8fdb\u53bb1\u4e2a\u8d28\u7c92\u5927\u6982\u7387\u53e6\u5916\u4e24\u4e2a\u4e5f\u80fd\u8fdb\u53bb\uff0c\u8f6c\u4e0d\u8fdb\u53bb\u8d28\u7c92\u7684\u7ec6\u80de\u5c311\u4e2a\u90fd\u8f6c\u4e0d\u8fdb\u53bb\u3002\u5373\uff0c\u591a\u8d28\u7c92\u8f6c\u67d3\u5e76\u4e0d\u662f\u76f8\u4e92\u72ec\u7acb\u7684\u3002<\/li>\n\n\n\n<li>\u5982\u679c\u6807\u51c6protocol\u662f\uff1a\u5bf9\u4e8e\u4e00\u4e2a\u517b\u57286- well plate\u7684\u7ec6\u80de\uff0c\u7528200ul opti-mem\u7a00\u91ca2ug\u8d28\u7c92\uff0c\u518d\u52a06ul Viafect\u3002\u6b64\u65f6\u4f60\u89813\u8d28\u7c92\u5171\u8f6c\u67d3\uff0c\u90a3\u53ef\u4ee5\u4ecd\u7136\u7528200ul Opti-MEM\u7a00\u91ca1.5+1.5+1.5\u4e00\u51714.5ug\u8d28\u7c92\uff0c\u518d\u52a013.5ul Viafect. \u8fd9\u6837\u6309\u7ecf\u9a8c\u6bd4\u7528450ul Opti-MEM\u8f6c\u67d3\u6548\u7387\u66f4\u9ad8\u3002<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd9\u7bc7\u7b14\u8bb0\u805a\u7126\u7ec6\u80de\u8f6c\u67d3\u7684\u5173\u952e\u7ec6\u8282\uff1a\u8f6c\u67d3\u524d\u7ec6\u80de\u72b6\u6001\uff08\u6362\u6db2\u4e0e\u5bc6\u5ea6\uff09\u3001\u8f6c\u67d3\u540e48\u5c0f\u65f6\u8fbe\u8868\u8fbe\u5cf0\u503c\uff0c\u5e76\u63a2\u8ba8\u4e86\u591a\u8d28\u7c92\u5171\u8f6c\u67d3\u7684\u975e\u72ec\u7acb\u6027\u73b0\u8c61\u4e0e\u4f18\u5316\u65b9\u6848\uff0c\u65e8\u5728\u63d0\u5347\u5b9e\u9a8c\u6548\u7387\u4e0e\u53ef\u91cd\u590d\u6027\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-1600","post","type-post","status-publish","format-standard","hentry","category-note"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/1600","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1600"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/1600\/revisions"}],"predecessor-version":[{"id":14117,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/1600\/revisions\/14117"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1600"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1600"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1600"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}