{"id":174,"date":"2023-03-28T02:12:58","date_gmt":"2023-03-27T18:12:58","guid":{"rendered":"http:\/\/39.105.184.3\/?p=174"},"modified":"2025-12-21T01:06:29","modified_gmt":"2025-12-20T17:06:29","slug":"co-ip%e5%ae%9e%e9%aa%8c%e7%bb%86%e8%8a%82","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=174","title":{"rendered":"Co-IP\u5b9e\u9a8c\u7ec6\u8282"},"content":{"rendered":"\n
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    1. \u786e\u8ba4\u76ee\u7684\u86cb\u767d\u6b63\u5e38\u8868\u8fbe<\/li>\n\n\n\n
    2. WB\u6297\u4f53\u53ef\u4ee5\u6210\u529f\u68c0\u6d4b\u5230\u76ee\u7684\u6761\u5e26<\/li>\n\n\n\n
    3. \u786e\u8ba4\u76ee\u7684\u6761\u5e26\u4f4d\u7f6e<\/li>\n<\/ol>\n<\/li>\n\n\n\n
    4. \u9634\u6027\u5bf9\u7167\u662f\u4e0eIP\u6297\u4f53\u540c\u79cd\u5c5e\u7684Normal IgG\uff0c\u76ee\u7684\u662f\u6392\u9664\u975e\u7279\u5f02\u6027\u6761\u5e26\u7684\u5e72\u6270\u3002\"image-20230328212711232\"<\/li>\n\n\n\n
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    6. \u540c\u4e0a\u4e00\u6761\u7684\u539f\u56e0\uff0c\u505aCo-IP\u65f6\u88c2\u89e3\u6db2\u7684\u9009\u62e9\u5f88\u91cd\u8981\uff0c\u4f7f\u7528\u975e\u53d8\u6027\u6e29\u548c\u88c2\u89e3\u6db2<\/code>\uff0c\u4ee5\u514d\u7834\u574f\u86cb\u767d-\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u3002\n
        \n
      1. \u907f\u514d\u4f7f\u7528\u79bb\u5b50\u578b\u53bb\u57a2\u5242\uff08\u5982SDS\uff09\uff0c\u86cb\u767d\u53d8\u6027\uff0c\u7834\u574f\u86cb\u767d-\u86cb\u767d\u76f8\u4e92\u4f5c\u7528<\/li>\n\n\n\n
      2. \u4f7f\u7528\u6e29\u548c\u975e\u79bb\u5b50\u578b\u53bb\u57a2\u5242\uff08\u5982NP-40, Triton X-100\u7b49\uff09<\/li>\n\n\n\n
      3. \u751f\u7406\u79bb\u5b50\u6d53\u5ea6\uff08\u5982150 mM NaCl\uff09<\/li>\n\n\n\n
      4. \u5c3d\u91cf\u907f\u514d\u5267\u70c8\u8d85\u58f0\u548c\u6da1\u65cb\u5904\u7406<\/li>\n<\/ol>\n<\/li>\n\n\n\n
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          \n
        1. Protein A\u548cProtein G\u5bf9\u4e0d\u540c\u79cd\u7c7b\u7684\u6297\u4f53\u7ed3\u5408\u80fd\u529b\u6709\u5dee\u5f02\uff0cProtein A\/G\u517c\u5177Protein A, G\u7684\u7ed3\u5408\u4f4d\u70b9\u3002<\/li>\n\n\n\n
        2. MRZ\u5e08\u5144\u9047\u5230\u8fc7Binding Buffer\u4e2dNaCl\u6d53\u5ea6\u8fc7\u9ad8\u5bfc\u81f4IP\u86cb\u767d\u7ed3\u5408\u4e0d\u5230\u6297\u4f53\u4e0a\u7684\u95ee\u9898\uff0c\u964d\u4f4eNaCl\u6d53\u5ea6\u540e\u5c31\u53ef\u4ee5\u4e86\u3002<\/li>\n<\/ol>\n<\/li>\n\n\n\n
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