{"id":182,"date":"2023-04-11T02:16:40","date_gmt":"2023-04-10T18:16:40","guid":{"rendered":"http:\/\/39.105.184.3\/?p=182"},"modified":"2025-12-21T01:06:27","modified_gmt":"2025-12-20T17:06:27","slug":"in-fusion-cloning%e4%b9%9f%e6%98%af%e7%8e%84%e5%ad%a6","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=182","title":{"rendered":"In-fusion cloning\u4e5f\u662f\u7384\u5b66"},"content":{"rendered":"\n<p>\u65e0\u7f1d\u514b\u9686\u8fde\u63a5\u4e00\u4e2a\u8d28\u7c92\uff0c\u52a0\u4e0a\u8f7d\u4f53\u4e00\u5171\u4e09\u4e2a\u7247\u6bb5\uff0c\u5206\u522b4kb\u591a\u30015kb\u591a\u548c800\u591abp\uff0c\u8bd5\u4e86\u4e09\u4e2a\u724c\u5b50\uff08Vanzyme\u7684Clone Express\u3001NEB\u7684NEBuilder Hifi assembly\u3001\u805a\u5408\u7f8e\u7684M5 compact\uff09\u90fd\u6ca1\u8fde\u8d77\u6765\u3002\u7136\u540e\u60f3\u4e86\u60f3In-fusion\u7684\u539f\u7406\uff0c\u89c9\u5f97\u5927\u6982\u7387\u662f5&#8217;\u21923&#8217;\u5916\u5207\u66b4\u9732\u51faDNA\u5355\u94fe\u540e\u4ea7\u751f\u4e86\u4e8c\u7ea7\u7ed3\u6784\u3002\u6bd5\u7adf\u53cd\u5e94\u6e29\u5ea6\u662f50\u2103\uff0c\u6bd4\u6b63\u5e38PCR\u4f4e\u591a\u4e86\u3002\u800c20\u591abp\u7684\u540c\u6e90\u81c2\u9000\u706b\u6e29\u5ea6\u5927\u6982\u572860\u2103\uff0c\u53ef\u80fd\u5f62\u6210\u4e8c\u7ea7\u7ed3\u6784\u5f71\u54cd\u540c\u6e90\u81c2\u4e4b\u95f4\u7684\u9000\u706b\u4e86\u5427\u3002\u627e\u4e86\u4e2a\u7f51\u7ad9\u9884\u6d4b\u4e86\u4e00\u4e0b\u540c\u6e90\u81c2\u5355\u94fe\u7684\u25b3G\uff1a<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202304112356304.png?x-oss-process=style\/screenshots\" alt=\"image-20230411235606794\" \/><\/figure>\n\n\n\n<p>\u4e0a\u9762\u4e09\u6761\u662f\u4e4b\u524d\u7684\uff0c\u4e0b\u9762\u4e09\u6761\u662f\u7528\u5f15\u7269\u91cd\u65b0PCR\uff0c\u628a\u4e09\u4e2a\u540c\u6e90\u81c2\u90fd\u5411\u5de6\u8fb9\u6216\u8005\u53f3\u8fb9\u5e73\u79fb\u4e00\u5c0f\u6bb5\u8ddd\u79bb\uff0c\u6362\u4e00\u6362\u5e8f\u5217\u3002<\/p>\n\n\n\n<p>\u7136\u540e\u4e00\u6b21\u5c31\u8fde\u8d77\u6765\u4e86\u3002<\/p>\n\n\n\n<p>\u8fd8\u6709\u4e2a\u7528In-fusion\u514b\u9686\u7684\u5c0f\u95ee\u9898\u6211\u4eca\u5929\u624d\u6ce8\u610f\u5230\u3002\u5982\u679c\u8bf4\u539f\u7406\u662f5&#8217;\u21923&#8217;\u5916\u5207\u9176\u5207\u51fa\u5355\u94fe\uff0c\u7136\u540e\u5230\u611f\u53d7\u6001\u5185\u90e8\u4fee\u590d\u7684\u8bdd\uff0c\u90a3\u4e48\u5982\u679c\u8fde\u63a5\u65f6\u95f4\u8fc7\u957f\uff0c\u5c31\u4f1a\u5bfc\u81f4\u5355\u94fe\u66b4\u9732\u533a\u57df\u8fc7\u5927\uff0c\u4e00\u65b9\u9762\u5f62\u6210\u4e8c\u7ea7\u7ed3\u6784\u7684\u53ef\u80fd\u8d8a\u9ad8\uff0c\u53e6\u4e00\u65b9\u9762\u4fee\u590d\u51fa\u9519\u7684\u6982\u7387\u4e5f\u8d8a\u9ad8\uff0c\u6240\u4ee5\u4e0d\u5e94\u60f3\u5f53\u7136\u89c9\u5f97\u65f6\u95f4\u957f\u4e00\u70b9\u8fde\u63a5\u6548\u7387\u66f4\u9ad8\uff0c\u5e94\u8be5\u4e25\u683c\u6309\u7167\u8bf4\u660e\u4e66\u63a8\u8350\u65f6\u95f4\u6765\u8fde\u63a5\u3002<\/p>\n\n\n\n<p>\u53c2\u8003\uff1a<a href=\"https:\/\/www.snapgene.com\/guides\/in-fusion-cloning\">In-Fusion Cloning &#8211; Snapgene<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd9\u7bc7\u7b14\u8bb0\u8bb0\u5f55\u4e86\u4f5c\u8005\u5728In-fusion\u514b\u9686\u5b9e\u9a8c\u4e2d\u9047\u5230\u7684\u8fde\u63a5\u5931\u8d25\u95ee\u9898\u3002\u901a\u8fc7\u5206\u6790\u53cd\u5e94\u539f\u7406\uff0c\u63a8\u6d4b\u662f50\u2103\u53cd\u5e94\u6e29\u5ea6\u4e0b\uff0c\u540c\u6e90\u81c2\u5355\u94fe\u6613\u5f62\u6210\u4e8c\u7ea7\u7ed3\u6784\u963b\u788d\u9000\u706b\u3002\u9a8c\u8bc1\u6027\u5730\u8c03\u6574\u540c\u6e90\u81c2\u5e8f\u5217\u540e\uff0c\u514b\u9686\u6210\u529f\uff0c\u5e76\u53cd\u601d\u4e86\u8fde\u63a5\u65f6\u95f4\u8fc7\u957f\u53ef\u80fd\u5e26\u6765\u7684\u98ce\u9669\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-182","post","type-post","status-publish","format-standard","hentry","category-note"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/182","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=182"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/182\/revisions"}],"predecessor-version":[{"id":14168,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/182\/revisions\/14168"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=182"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=182"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=182"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}