{"id":193,"date":"2023-07-13T02:26:46","date_gmt":"2023-07-12T18:26:46","guid":{"rendered":"http:\/\/39.105.184.3\/?p=193"},"modified":"2025-12-21T01:06:23","modified_gmt":"2025-12-20T17:06:23","slug":"rna%e5%8f%98%e6%80%a7%e8%83%b6","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=193","title":{"rendered":"RNA\u53d8\u6027\u80f6"},"content":{"rendered":"\n

\u7b2c\u4e00\u6b21\u505aRNA\u53d8\u6027\u80f6\uff0c\u8bb0\u70b9\u4e1c\u897f\u3002<\/p>\n\n\n\n

RNA\u4e8c\u7ea7\u7ed3\u6784\u5f88\u590d\u6742\uff0c \u6240\u4ee5\u5728\u975e\u53d8\u6027\u6761\u4ef6\u4e0b\u8dd1\u80f6\u4e0d\u80fd\u6b63\u786e\u53cd\u6620\u5927\u5c0f\u3002\u53d8\u6027\u80f6\u6709\u743c\u8102\u7cd6+\u7532\u919b\u7684\uff0c\u8fd8\u6709\u805a\u4e19\u70ef\u9170\u80fa+\u5c3f\u7d20\u7684\u3002\u524d\u8005\u5206\u79bb\u8303\u56f4\u8f83\u5927\uff0c\u540e\u8005\u5206\u8fa8\u7387\u9ad8\uff0c\u4e3b\u8981\u7528\u4e8e1500bp\u4ee5\u4e0b\u7684RNA\u3002<\/p>\n\n\n\n

\"image-20230713014414198\"<\/figure>\n\n\n\n

\u6211\u7528\u76841xTBE\u505aRunning Buffer\uff0c\u4e4b\u540e\u67d3\u8272\u7528\u76841xTBE\u52a0\u4e07\u5206\u4e4b\u4e00\u7684SYBR Gold\uff0c\u5ba4\u6e29\u5b75\u80b215min\u3002\u7ed3\u679c\u66dd\u5149\u51fa\u6765\u964d\u89e3\u8fd8\u662f\u975e\u5e38\u660e\u663e\u7684\u3002<\/p>\n\n\n\n

\u6ce8\u610f\u4e8b\u9879\uff1a<\/p>\n\n\n\n

    \n
  1. \u5c3f\u7d20\u6eb6\u89e3\u5438\u70ed\uff0c\u6240\u4ee5\u9002\u5f53\u52a0\u52a0\u70ed\u8ba9\u5b83\u6eb6\u89e3\u66f4\u5feb\u3002<\/li>\n\n\n\n
  2. RNA\u6837\u54c1\u52a0loading buffer\u540e\u8981\u73b0\u572890\u2103\u52a0\u70ed5min\uff0c\u7136\u540e\u8fc5\u901f\u7f6e\u4e8e\u51b0\u4e0a\u51b7\u5374\uff0c\u6253\u5f00\u4e8c\u7ea7\u7ed3\u6784\u3002RNA Ladder\u4e5f\u8981\u3002<\/li>\n\n\n\n
  3. \u62d4\u4e86\u68b3\u5b50\u4e4b\u540e\uff0c\u4e00\u5b9a\u8981\u51b2\u6d17\u5e72\u51c0\u5b54\u91cc\u7684\u5c3f\u7d20\uff0c\u4e0d\u7136\u6837\u54c1\u5f88\u96be\u5728\u7535\u573a\u4e0b\u5f80\u4e0b\u79fb\u52a8\uff0c\u800c\u4e14\u4e0d\u540c\u5b54\u4e2d\u7684\u6b8b\u7559\u5c3f\u7d20\u91cf\u4e0d\u540c\uff0c\u9020\u6210\u8fc1\u79fb\u7387\u4e5f\u4e0d\u540c\uff0c\u6761\u5e26\u4f1a\u53c2\u5dee\u4e0d\u9f50\uff0c\u8fd9\u4e00\u7ec6\u8282\u975e\u5e38\u91cd\u8981\u3002<\/li>\n\n\n\n
  4. 5%\u7684\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u975e\u5e38\u8f6f\uff0c\u5f88\u96be\u8f6c\u79fb\u4f4d\u7f6e\uff0c\u7a0d\u5fae\u4e00\u4e0d\u6ce8\u610f\u5c31\u7834\u4e86\uff0c\u6216\u8005\u53d8\u6210\u4e00\u5768\u6839\u672c\u5c55\u4e0d\u5f00\u3002\u6211\u662f\u628a\u4e00\u4e2a\u4e00\u6b21\u6027\u900f\u660e\u624b\u5957\u653e\u5728\u4e00\u4e2a\u5e73\u677f\u4e0a\uff0c\u6dcb\u4e00\u70b9\u6c34\uff0c\u7136\u540e\u628a\u80f6\u653e\u5728\u4e0a\u9762\u524d\u540e\u5de6\u53f3\u6643\uff0c\u76f4\u5230\u628a\u5b83\u6643\u5f00\u3001\u5c55\u5e73\u3002\u7136\u540e\u7aef\u7740\u624b\u5957\u8f6c\u79fb\u5230\u663e\u5f71\u4eea\u91cc\u3002<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    \u8fd9\u7bc7\u5b9e\u9a8c\u7b14\u8bb0\u8bb0\u5f55\u4e86\u9996\u6b21\u8fdb\u884cRNA\u53d8\u6027\u51dd\u80f6\u7535\u6cf3\u7684\u5173\u952e\u8981\u70b9\u3002\u4f5c\u8005\u8be6\u7ec6\u6bd4\u8f83\u4e86\u7532\u919b-\u743c\u8102\u7cd6\u4e0e\u5c3f\u7d20-\u805a\u4e19\u70ef\u9170\u80fa\u4e24\u79cd\u53d8\u6027\u7cfb\u7edf\u7684\u7279\u70b9\u4e0e\u9002\u7528\u573a\u666f\uff0c\u5e76\u5206\u4eab\u4e86\u4ece\u5236\u80f6\u3001\u6837\u54c1\u5904\u7406\u3001\u4e0a\u6837\u5230\u663e\u5f71\u7684\u5168\u6d41\u7a0b\u5b9e\u64cd\u7ecf\u9a8c\uff0c\u7279\u522b\u662f\u9488\u5bf9RNA\u4e8c\u7ea7\u7ed3\u6784\u53d8\u6027\u3001\u5c3f\u7d20\u6b8b\u7559\u5f71\u54cd\u53ca\u8f6f\u80f6\u5904\u7406\u7b49\u6613\u9519\u73af\u8282\u7ed9\u51fa\u4e86\u5177\u4f53\u89e3\u51b3\u65b9\u6848\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,4],"tags":[],"class_list":["post-193","post","type-post","status-publish","format-standard","hentry","category-uncategorized","category-note"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/193","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=193"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/193\/revisions"}],"predecessor-version":[{"id":14158,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/193\/revisions\/14158"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=193"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=193"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=193"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}