7.3<\/p>\n\n\n\n
\u524d\u4e00\u5929\u56e0\u4e3a\u901a\u5bb5\u505a\u4e86IVT\u8f6c\u5f55gRNA\u7684\u9884\u5b9e\u9a8c\u3002\u6240\u4ee5\u767d\u5929\u57fa\u672c\u5728\u7761\u89c9\u3002\u665a\u4e0a\u53bb\u6311\u4e86\u4e2a\u83cc\u3002\u7136\u540e21:00\u7ea6\u4e86\u4e0d\u523010\u4e2a\u4eba\u53bb\u7d2b\u8346\u5355\u6760\u7ec3\u4e86\u4e24\u7ec4\u6251\u514b\u724c\u5f15\u4f53\u3002<\/p>\n\n\n\n
7.4<\/p>\n\n\n\n
\u672c\u6765\u4e0a\u5348\u4e03\u70b9\u5c31\u9192\u4e86\uff0c\u800c\u4e14\u795e\u6e05\u6c14\u723d\uff0c\u4f46\u4e00\u60f3\u6211\u6447\u7684\u83cc\u5230\u4e5d\u70b9\u624d14\u4e2a\u5c0f\u65f6\uff0c\u5c31\u53c8\u7761\u8fc7\u53bb\u4e86\uff0c\u5341\u4e00\u70b9\u534a\u624d\u9192\u3002\u7136\u540e\u53bb\u6d17\u4e86\u4e2a\u6fa1\u5403\u4e86\u4e2a\u996d\u3002\u53bb\u5b9e\u9a8c\u5ba4\u628a\u83cc\u6db2\u4ece\u6447\u5e8a\u53d6\u51fa\u6765\u3002\u5341\u4e8c\u70b9\u534a\u5230\u8fbe\u65b0\u6c34\u5229\u9986\u627e\u6768\u65b0\u82d7\u8001\u5e08\uff0c\u6768\u8001\u5e08\u5f00\u8f66\u5e26\u6211\u4eec\u53bb\u4e86\u5317\u4eac\u5e02\u6d41\u57df\u4e2d\u5fc3\u3002\u5f00\u4f1a\u56de\u6765\u5c31\u56db\u70b9\u591a\u4e86\u3002\u63d0\u4e86\u4e2a\u8d28\u7c92\uff0c\u914d\u597d\u9176\u5207\u4f53\u7cfb\u3002\u7136\u540e\u53bb\u5403\u665a\u996d\u3002\u665a\u4e0a\u4e03\u70b9\u53bb\u4e1c\u64cd\u8dd1\u5eca\u8bad\u7ec3\uff0c\u56de\u6765\u662f\u4e5d\u70b9\u3002\u5f00\u59cb\u914d\u80f6\u3001\u8dd1\u80f6\u3001\u80f6\u56de\u6536\u3002<\/p>\n\n\n\n
\u4e2d\u95f4\u51e0\u5929\u6ca1\u6709\u8bb0\uff0c\u5fd8\u8bb0\u4e86\u3002\u603b\u4e4b\u662f\u53d1\u73b0\u81ea\u5df1\u4e70\u9519\u4e86\u8bd5\u5242\u76d2\uff0cIVT\u7684\uff0c\u5e94\u8be5\u4e70T7\u542f\u52a8\u5b50\u7684\uff0c\u7ed3\u679c\u4e70\u6210SP6\u7684\u4e86\u3002\u4e4b\u540e\u501f\u4e86\u5b5f\u8001\u5e08\u5b9e\u9a8c\u5ba4\u7684\u4e24\u4e2a\u53cd\u5e94\uff0c\u505a\u4e86IVT\uff0csgRNA\u7684IVT\u4e5f\u505a\u4e86\u3002\u8dd1\u4e86\u4e24\u6b21\u805a\u4e19\u70ef\u9170\u80fa-TBE-\u5c3f\u7d20\u80f6\u3002<\/p>\n\n\n\n
7.10<\/p>\n\n\n\n
\u4eca\u5929\u6691\u671f\u96c6\u8bad\u7b2c\u4e00\u5929\u3002\u8001\u677f\u8ba9\u505aMS2 stem loop – MCP(MS2 coat protein)\u6765\u627eL1 RNA\u7684interation\u86cb\u767d\u3002\u53d1\u73b0\u4eceAddgene\u4e70\u53c8\u8d35\u53c8\u6162\uff0c\u501f\u4e86\u597d\u51e0\u4e2a\u5b9e\u9a8c\u5ba4\u7684\u8d28\u7c92\uff0c\u6253\u7b97\u81ea\u5df1\u6784\u5efa\u4e86\u3002<\/p>\n\n\n\n
7.11<\/p>\n\n\n\n
\u7761\u5230\u4e2d\u5348\u5341\u4e8c\u70b9\uff0c\u73a9\u624b\u673a\u5230\u4e0b\u5348\u4e09\u70b9\u4e0a\u73ed\uff0c\u6311\u4e86\u83ccPCS2\u4f53\u5916\u8f6c\u5f55\u7684\uff0c\u9274\u5b9a\u4e86\u3002\u53cd\u8f6c\u5f55\u4e86IVT\u4ea7\u7269\uff0c\u51c6\u5907\u7eaf\u5316\u6d4b\u5e8f\u300219\u70b9\u53bb\u8bad\u7ec3\uff0c\u8fd9\u662f\u4e2a\u4eba\u6691\u8bad\u7684\u7b2c\u4e00\u6b21\u6253\u5361\uff0c\u4eca\u5929\u4e0d\u77e5\u9053\u4e3a\u5565\u51fa\u7684\u6c57\u7279\u522b\u591a\uff0c\u4e07\u5764\u4e5f\u662f\uff0c\u5168\u8eab\u90fd\u6e7f\u900f\u4e86\u3002\u665a\u4e0a\u7ec3\u5b8c\u548c\u8c2d\u4eba\u8c6a\u4e00\u8d77\u53bb\u6843\u674e\u5403\u4e86\u4e2a\u897f\u74dc\uff0c\u56de\u5bbf\u820d\u6d17\u6fa1\u3002\u6d17\u5b8c\u6fa1\u53d1\u73b0\u96e8\u4e0b\u5f97\u597d\u5927\uff0c\u5c31\u4e0d\u60f3\u56de\u5b9e\u9a8c\u5ba4\u4e86\u3002\u665a\u4e0a\u5341\u4e00\u70b9\u591a\u70b9\u4e86\u4e2a\u87ba\u86f3\u7c89\u3002<\/p>\n\n\n\n
7.12<\/p>\n\n\n\n
\u524d\u4e00\u5929\u53c8\u73a9\u624b\u673a\uff0c\u7761\u5230\u4e2d\u5348\u5341\u4e8c\u70b9\uff0c\u73a9\u624b\u673a\u5230\u4e0b\u5348\u56db\u70b9\uff0c\u5728\u5c0f\u6865\u5934\u4e70\u4e86\u4e2a\u70e4\u80a0\u53bb\u5b9e\u9a8c\u5ba4\uff0c\u628a\u6628\u5929PCR\u4ea7\u7269\u8dd1\u4e86\u80f6\uff0c\u53d1\u73b0\u90fd\u5bf9\uff0c\u9001\u4e86\u6d4b\u5e8f\u3002\u8fc7\u67f1\u7eaf\u5316\u4e86IVT\u7684\u53cd\u8f6c\u5f55\u4ea7\u7269\uff0c\u56e0\u4e3a\u4e0d\u77e5\u9053\u91cc\u9762\u6b8b\u7559\u7684\u516d\u5f15\u7269\u7b49\u4f1a\u4e0d\u4f1a\u5f71\u54cd\u4e00\u4ee3\u6d4b\u5e8f\u3002\u4e0b\u5348\u516d\u70b9\u591a\u51af\u8001\u5e08\u53eb\u6211\u548c\u5e2d\u6cfd\u53bb\u9648\u660e\u9986\u804a\u4e86\u4e00\u4e2a\u534a\u5c0f\u65f6\u3002\u5e2d\u6cfd\u88ab\u4efb\u547d\u4e3a\u6559\u7ec3\u4e86\u3002\u8ba8\u8bba\u4e86\u4e00\u4e9b\u4e8b\u60c5\uff0c\u6bd4\u8d5b\u7684\u8bdd\uff0c\u9996\u9ad8\u7b2c\u4e00\uff0c\u5168\u56fd\u5927\u5b66\u751f\u9526\u6807\u8d5b\u7b2c\u4e8c\u3002\u4f18\u5148\u5927\u5b66\u751f\u4f53\u80b2\u534f\u4f1a\u4e3b\u529e\u7684\u6bd4\u8d5b\uff0c\u5176\u6b21\u4f53\u80b2\u603b\u5c40\u4e3b\u529e\u7684\uff0c\u518d\u6b21\u5927\u5b66\u4e3e\u529e\u7684\u3002\u4e3b\u8981\u770b\u4e3b\u529e\u548c\u627f\u529e\uff0c\u4e3b\u529e\u548c\u627f\u529e\u662f\u5546\u4e1a\u516c\u53f8\u7684\u4e0d\u80fd\u53c2\u8d5b\u3002\u653f\u5e9c\u4e3b\u529e\u7684\u53ef\u4ee5\u9009\u62e9\u6027\u53c2\u52a0\uff0c\u53cd\u6b63\u5b66\u6821\u4e00\u5206\u94b1\u4e0d\u7ed9\u3002\u4e5d\u6c5f\u96c6\u8bad\u7684\u4e8b\u60c5\u5b66\u6821\u53ef\u80fd\u4e5f\u4e0d\u7ed9\u94b1\uff0c\u51af\u8001\u5e08\u8bf4\u53ef\u4ee5\u627e\u6821\u53cb\u8d5e\u52a9\uff0c\u4e0d\u8981\u4f01\u4e1a\u8d5e\u52a9\u3002\u8ba9\u60e0\u60e0\u62ff\u7740\u7968\u636e\u627e\u4ed6\u7b7e\u5b57\uff0c\u7136\u540e\u53bb\u4f53\u80b2\u90e8\u62a5\u9500\u3002<\/p>\n\n\n\n
7.14<\/p>\n\n\n\n
\u665a\u4e0a\u7ec4\u4f1a\u6211\u5565\u90fd\u6ca1\u542c\uff0cZT\u8bb2\u7684\u6587\u732e\u6211\u4e5f\u6ca1\u542c\u3002\u7ec4\u4f1a\u4e00\u7ed3\u675f\u5c31\u98de\u5feb\u7684\u8dd1\u56de\u5bbf\u820d\u4e86\u3002<\/p>\n\n\n\n
7.15~7.16<\/p>\n\n\n\n
\u8fd9\u4e24\u5929\u662f\u5468\u516d\u65e5\uff0c\u4e5f\u4e00\u70b9\u4e8b\u60c5\u6ca1\u5e72\u3002\u800c\u4e14\u53d1\u73b0\u628atRNA ligation complex\u7684\u51e0\u4e2aGFP\u6362\u6210tagBFP\u7684\u6784\u5efa\u56e0\u4e3a\u4e00\u6b65\u64cd\u4f5c\u5fd8\u8bb0\u4e86\u7ed3\u679c\u6d4b\u5e8f\u5168\u4e0d\u5bf9\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"
\u4e3b\u4eba\u8fd9\u5468\u7684\u5b9e\u9a8c\u5ba4\u65e5\u5e38\u771f\u662f\u8dcc\u5b95\u8d77\u4f0fwwww\uff0c\u4ece\u901a\u5bb5\u505aIVT\u9884\u5b9e\u9a8c\u3001\u4e70\u9519\u8bd5\u5242\u76d2\uff0c\u5230\u6784\u5efaMS2-MCP\u8d28\u7c92\u3001\u8dd1\u80f6\u7eaf\u5316\uff0c\u79d1\u7814\u751f\u6d3b\u6ee1\u6ee1\u5f53\u5f53\u3002\u8bad\u7ec3\u4e5f\u6ca1\u843d\u4e0b\uff0c\u7d2b\u8346\u5355\u6760\u3001\u4e1c\u64cd\u8dd1\u5eca\uff0c\u6c57\u6c34\u4e0e\u87ba\u86f3\u7c89\u9f50\u98de\uff5e\u5076\u5c14\u7684\u6478\u9c7c\u548c\u5b9e\u9a8c\u5c0f\u63d2\u66f2\uff0c\u624d\u662f\u771f\u5b9e\u7684\u7814\u7a76\u751f\u65e5\u5e38\u561b\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"class_list":["post-197","post","type-post","status-publish","format-standard","hentry","category-blog"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/197","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=197"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/197\/revisions"}],"predecessor-version":[{"id":14161,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/197\/revisions\/14161"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=197"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=197"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=197"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}