6.19<\/p>\n\n\n\n
\u4e0a\u5348\u8d56\u5e8a\uff0c\u5237\u4e86\u4e09\u96c6\u6597\u7f57\u5927\u9646\u3002<\/p>\n\n\n\n
\u4e0b\u5348\u53bbibp\u7ed9\u80af\u5fb7\u57fa\u9001\u4e86\u8863\u670d\uff0c\u628a\u5316\u5b66\u5965\u8d5b\u5fd7\u613f\u8005\u8bc1\u4e66\u62ff\u56de\u6765\u4e86\u3002<\/p>\n\n\n\n
\u505a\u4e86\u6311\u83cc\u9a8c\u8bc1\u3002<\/p>\n\n\n\n
\u665a\u4e0a\u7ec3\u4e86200\u4e2a\u5f15\u4f53\u3002<\/p>\n\n\n\n
6.20<\/p>\n\n\n\n
\u4e0b\u5348\u56db\u70b9\u624d\u8d77\u5e8a\u51fa\u95e8\uff0c\u65e9\u4e0a\u5341\u4e00\u70b9\u9192\u7684\uff0c\u524d\u4e00\u5929\u665a\u4e0a\u53bb\u548csyy\u5403\u591c\u5bb5\u5230\u4e09\u70b9\u534a\u3002\u4e2d\u95f4\u51e0\u4e2a\u5c0f\u65f6\u53ef\u80fd\u5728\u73a9\u624b\u673a\u3002\u51fa\u95e8\u540e\u53bb\u897f\u4f53\u8003\u5bdf\u4e86\u4e00\u4e0b\u5065\u8eab\u623f\u3002<\/p>\n\n\n\n
\u4e0b\u5348\u5199\u4e86\u4e00\u4efd\u8bad\u7ec3\u8ba1\u5212\uff0c\u665a\u4e0a\u4e03\u70b9\u5230\u4e5d\u70b9\u534a\u53bb\u7ec3\u4e86\u3002\u7136\u540e\u53bb\u6843\u674e\u5403\u4e86\u4e2a\u591c\u5bb5\u3002<\/p>\n\n\n\n
\u5341\u70b9\u534a\u5230\u5341\u4e8c\u70b9\u5e94\u4ed8\u4e86RNA\u751f\u7269\u5b66\u7684\u4f5c\u4e1a\u3002<\/p>\n\n\n\n
6.21<\/p>\n\n\n\n
6.22<\/p>\n\n\n\n
\u4eca\u5929\u662f\u7aef\u5348\u8282\uff0c\u65e9\u4e0a\u53bb\u5706\u660e\u56ed\u6bd4\u8d5b\u3002\u4e0b\u5348\u548c\u9ec4\u5609\u60e0\u53bb\u4e00\u5929\u81ea\u7136\u5e26\u9f99\u821f\u4f53\u9a8c\u6d3b\u52a8\u3002\u665a\u4e0a\u516b\u70b9\u53bb\u51d1\u4e86\u4e2a\u665a\u8bad\u7684\u5c3e\u5df4\uff0c\u548c\u6731\u5c1a\u81fb\u6bd4\u4e86\u5f15\u4f53\uff0c\u7136\u540e\u4e00\u4f19\u4eba\u53bb\u6843\u674e\u5403\u4e86\u597d\u591a\u74dc\u3002<\/p>\n\n\n\n
6.23<\/p>\n\n\n\n
\u7761\u89c9\uff0c\u8eba\u5e8a\u4e0a\u73a9\u624b\u673a\uff0c\u5403\u5916\u5356\u3002<\/p>\n\n\n\n
6.24<\/p>\n\n\n\n
\u7761\u89c9\uff0c\u8eba\u5e8a\u4e0a\u73a9\u624b\u673a\uff0c\u5403\u5916\u5356\u3002\u665a\u4e0a\u53bb\u897f\u4f53\u548c\u82cf\u5609\u66e6\u7ec3\u4e86\u80a9\u80cc\u3002<\/p>\n\n\n\n
6.25<\/p>\n\n\n\n
\u53bb\u505a\u4e86\u4e00\u70b9\u5b9e\u9a8c\u3002\u6311\u83cc\uff08IVT\u8d28\u7c92\uff09\u3001PCR\u9274\u5b9a\u3001\u9001\u6d4b\u5e8f\u3001\u63d0\u8d28\u7c92\u3002\u7136\u540e\u5f88\u65e9\u53c8\u56de\u5bbf\u820d\u7761\u89c9\u4e86\u3002<\/p>\n\n\n\n
6.26<\/p>\n\n\n\n
\u867d\u7136\u524d\u4e00\u5929\u5f88\u65e9\u56de\u53bb\uff0c\u4f46\u73a9\u624b\u673a\u5230\u4e09\u70b9\u534a\uff0c\u7761\u5230\u65e9\u4e0a\u5341\u4e00\u70b9\u534a\uff0c\u53bb\u63a5\u5e05\u9f99\uff0c\u8ddf\u4ed6\u53bb\u5706\u660e\u56ed\u8f6c\u4e86\u4e00\u5708\uff0c\u7136\u540e\u5728\u671b\u4eac\u4e32\u5427\u5403\u4e86\u4e00\u987f\u70e7\u70e4\uff0c\u7136\u540e\u53bb\u7f51\u5427\u6253\u4e86\u4e24\u4e2a\u5c0f\u65f6\u7ea2\u8b66\uff0c\u7136\u540e\u53bb\u4ed6\u7684\u9152\u5e97\u7761\u4e86\u4e00\u89c9\u3002<\/p>\n\n\n\n
6.27<\/p>\n\n\n\n
\u7761\u5230\u5341\u4e00\u70b9\u534a\uff0c\u5728\u6c99\u53bf\u5c0f\u5403\u5403\u4e86\u4e00\u987f\uff0c\u7136\u540e\u4ed6\u56de\u592a\u539f\u627e\u5973\u670b\u53cb\uff0c\u6211\u56de\u5b9e\u9a8c\u5ba4\u3002<\/p>\n\n\n\n
\u628asgRNA\u5f15\u7269\u4e70\u4e86\u3002\u6d4b\u5e8f\u6d4b\u901a\u3002\u6447\u4e2a\u83cc\u3002\u770b\u4e86\u4e00\u4e0b\u8001\u677f\u53d1\u7684manuscript\uff0c\u4f46\u6211\u505a\u4e0d\u51fa\u5565\u4fee\u6539\u610f\u89c1\u3002<\/p>\n\n\n\n
6.28<\/p>\n\n\n\n
IVT\u4e24\u4e2a\u8d28\u7c92\u90fd\u6784\u51fa\u6765\u4e86\u3002<\/p>\n\n\n\n
\u5927\u59d1\u4e00\u5bb6\u56db\u53e3\u4e09\u4ee3\u4eba\u6765\u5317\u4eac\uff0c\u5e26\u4ed6\u4eec\u53bb\u4e86\u5317\u5927\u548c\u6e05\u534e\u3002<\/p>\n\n\n\n
6.29<\/p>\n\n\n\n
IVT\u8d28\u7c92\u63d0\u4e86\u3002\u5f00\u59cbPCR gRNA\u7684\u6a21\u677f\u3002<\/p>\n\n\n\n
\u665a\u4e0a\u7ec3\u817f\u548c\u8170\u3002\u817f\u5e9f\u4e86\u3002<\/p>\n\n\n\n
6.30<\/p>\n\n\n\n
\u6211\u662f\u5e9f\u7269\uff0c\u672c\u6765\u524d\u4e00\u5929\u6253\u7b97\u5e26\u7535\u8111\u56de\u53bb\u5e72\u70b9\u6b63\u4e8b\u7684\uff0c\u7ed3\u679c\u90fd\u6ca1\u5f00\u673a\uff0c\u73a9\u624b\u673a\u5230\u4e09\u70b9\uff0c\u7136\u540e\u7761\u5230\u4e2d\u5348\u5341\u4e8c\u70b9\u3002\u70b9\u4e86\u5916\u5356\uff0c\u6d17\u4e86\u6fa1\u3001\u8863\u670d\u3001\u978b\u3002<\/p>\n\n\n\n
\u4e0b\u5348\u53bb\u8dd1\u4e86\u4e2a\u80f6\u3002<\/p>\n\n\n\n
\u665a\u4e0a\u5f00\u7ec4\u4f1a\u3002<\/p>\n\n\n\n
7.1<\/p>\n\n\n\n
\u665a\u4e0a\u53bbImagine Bar\u901a\u5bb5\u559d\u4e86\u7cbe\u917f\uff0c\u7136\u540e\u5403\u4e86\u70b9\u70e7\u70e4\uff0c\u7136\u540e\u56de\u53bb\u5c31\u51cc\u6668\u56db\u70b9\u4e86\u3002<\/p>\n\n\n\n
7.2<\/p>\n\n\n\n
\u7761\u4e86\u4e00\u6574\u5929\u3002\u665a\u4e0a\u53bb\u505a\u4e86IVT\u7684\u4e00\u70b9\u9884\u5b9e\u9a8c\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"
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