6.10<\/p>\n\n\n\n
\u9996\u9ad8\u8d5b\u524d\u53bb\u540e\u6d77\u6700\u540e\u4e00\u7ec3<\/p>\n\n\n\n
\u665a\u4e0a\u505a\u4e86\u652f\u539f\u4f53\u68c0\u6d4b\u7684PCR<\/p>\n\n\n\n
6.11<\/p>\n\n\n\n
\u9996\u9ad8\u6bd4\u8d5b\uff0c\u6bd4\u4e86\u4e00\u4e0a\u5348\u3002\u665a\u4e0a\u987a\u52bf\u559d\u4e86\u5c0f\u9152\u3002<\/p>\n\n\n\n
\u805a\u9910\u524d\u628a\u524d\u4e00\u5929PCR\u7684\u4ea7\u7269\u8dd1\u4e86\u80f6<\/p>\n\n\n\n
6.12<\/p>\n\n\n\n
\u627e\u4e86\u6768\u65b0\u82d7\u8001\u5e08\u9762\u8c08\u8ddf\u5317\u4eac\u5e02\u6c34\u52a1\u5c40\u534f\u8c03\u8bad\u7ec3\u573a\u5730\u7684\u4e8b\u60c5\u3002<\/p>\n\n\n\n
\u665a\u4e0a\u53bb\u4e86\u5317\u5927\uff0c\u8ddf\u7740\u4ed6\u4eec\u7684\u9f99\u821f\u961f\u8bad\u7ec3\u4e86\u4e00\u6b21\uff0c\u8fd8\u88ab\u8bf7\u5403\u4e86\u4e00\u987f\u706b\u9505\uff08\u8fd9\u8f88\u5b50\u7b2c\u4e00\u6b21\u8fdb\u71d5\u56ed\uff09\u3002<\/p>\n\n\n\n
\u505a\u4e86\u4e00\u70b9L1 deletion\u7684PCR\u9a8c\u8bc1\u3002<\/p>\n\n\n\n
\u628a\u5728\u51b0\u7bb1\u91cc\u653e\u4e86\u597d\u51e0\u5929\u7684\u5207\u80f6\u7ed9\u56de\u6536\u4e86\uff0c\u6000\u7591\u8fd8\u80fd\u4e0d\u80fd\u7528<\/p>\n\n\n\n
6.13<\/p>\n\n\n\n
\u4e0a\u5348\u8d56\u5e8a\u4e86\uff0c\u73a9\u4e86\u4e00\u4e0a\u5348\u8352\u91ce\u4e71\u6597\u3002<\/p>\n\n\n\n
\u665a\u4e0a\u5728\u8dd1\u5eca\u8bad\u7ec3\u3002<\/p>\n\n\n\n
\u628aRIP\u7684\u7247\u6bb5\u5206\u9009\u505a\u4e86<\/p>\n\n\n\n
6.14<\/p>\n\n\n\n
\u7ec4\u7ec7\u4e3a\u961f\u91cc\u76842023\u5c4a\u6bd5\u4e1a\u751f\u9001\u884c\uff0c\u7edf\u8ba1\u65f6\u95f4\u3002<\/p>\n\n\n\n
\u665a\u4e0a\u53bb\u5355\u53cc\u6760\u7ec3\u4e86\u5f15\u4f53\u3002<\/p>\n\n\n\n
\u4e2d\u5348\u88ab\u51af\u8001\u5e08\u7ea6\u8c08\uff0c\u8bf4\u6211\u4e0d\u8be5\u81ea\u4f5c\u4e3b\u5f20\u6d89\u624b\u5546\u4e1a\u5408\u4f5c\u3002<\/p>\n\n\n\n
\u6311\u83cc\u9274\u5b9a\u6ca1\u95ee\u9898\uff0c\u9001\u6d4b\u4e86\uff0c\u4f46\u662f\u65e0\u4fe1\u53f7\u3002<\/p>\n\n\n\n
6.15<\/p>\n\n\n\n
\u628aRIP\u7684PCR\u6587\u5e93\u6269\u589e\u505a\u4e86\u3002<\/p>\n\n\n\n
\u665a\u4e0a\u53bb\u8dd1\u5eca\u8bad\u7ec3\u3002<\/p>\n\n\n\n
6.16<\/p>\n\n\n\n
\u505a\u4e86RNA-seq\u7684\u4e00\u534a\u3002<\/p>\n\n\n\n
\u665a\u4e0a\u5355\u6760\u62c9\u4e86150\u4e2a\u5f15\u4f53\u3002<\/p>\n\n\n\n
\u7ed9\u6bd5\u4e1a\u751f\u51c6\u5907\u793c\u7269\uff0c\u6dd8\u5b9d\u548c\u4eac\u4e1c\u505a\u4e86\u5b9a\u5236\u7684\u5e3d\u5b50\u548c\u8863\u670d\u3002<\/p>\n\n\n\n
\u628aRIP\u7684PCR\u4ea7\u7269\u56de\u6536\u4e86\u3002<\/p>\n\n\n\n
6.17<\/p>\n\n\n\n
\u505a\u4e86RNA-seq\u7684\u53e6\u4e00\u534a\uff0c\u4f46\u662f\u6ca1\u6765\u5f97\u53ca\u9001\u51fa\u53bb\u3002<\/p>\n\n\n\n
\u5728\u5fd7\u5b8f\u7684\u65bd\u538b\u4e0b\u5f00\u59cb\u7ec4\u7ec7\u6691\u671f\u96c6\u8bad\u7684\u4e8b\u60c5\u3002<\/p>\n\n\n\n
6.18<\/p>\n\n\n\n
\u9001\u51fa\u53bb\u4e86RNA-seq\u548cRIP-seq\u6587\u5e93\u3002<\/p>\n\n\n\n
\u5f00\u59cb\u505a\u7b2c\u4e8c\u6b21\u6784\u8d28\u7c92\u3002<\/p>\n\n\n\n
\u4ed3\u4fc3\u5730\u5728ddl\u524d\u7528\u5728\u7ebf\u8f6f\u4ef6\u5199\u5b8c\u4e86\u4ee3\u8c22\u7ec4\u5b66\u7684\u671f\u672b\u4f5c\u4e1a\u3002<\/p>\n\n\n\n
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