{"id":205,"date":"2023-07-22T02:31:01","date_gmt":"2023-07-21T18:31:01","guid":{"rendered":"http:\/\/39.105.184.3\/?p=205"},"modified":"2025-12-21T01:06:20","modified_gmt":"2025-12-20T17:06:20","slug":"fana%e5%92%8cmorpholino%e4%b8%a4%e7%a7%8daso%e7%9a%84%e5%8c%ba%e5%88%ab","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=205","title":{"rendered":"FANA\u548cMorpholino\u4e24\u79cdASO\u7684\u533a\u522b"},"content":{"rendered":"\n<p>FANA\u548cMorpholino\u90fd\u662f\u901a\u8fc7\u4e0e\u76ee\u7684RNA\u53cd\u5411\u4e92\u8865\u53d1\u6325\u4f5c\u7528\u7684Knockdown\u65b9\u6cd5\uff0c\u524d\u8005\u4f9d\u8d56\u4e8eRNase H\u5bf9\u6742\u4ea4\u94fe\u4e2dRNA\u7684\u964d\u89e3\u5b9e\u73b0\u6572\u964d(RNase H-dependent)\uff0c\u540e\u8005\u4e0d\u4f9d\u8d56\u4e8eRNaseH\uff0c\u4f9d\u9760\u4e0e\u9776RNA\u7d27\u5bc6\u7ed3\u5408\u540e\u7684\u7a7a\u95f4\u4f4d\u963b\u6291\u5236\u7ffb\u8bd1\u548c\u526a\u63a5\u5b9e\u73b0\u6572\u964d(RNase H-independent)\u3002<\/p>\n\n\n\n<h1 class=\"wp-block-heading\">FANA<\/h1>\n\n\n\n<p>\u4e3a\u4e86\u66f4\u597d\u7684\u8bf4\u660eFANA\u7684\u7279\u70b9\uff0c\u5148\u4ecb\u7ecd\u548c\u5b83\u76f8\u5173\u7684\u5176\u4ed6\u79cd\u7c7b\u7684ASOs\u3002<\/p>\n\n\n\n<p>\u5df2\u77e5DNA-RNA hybrid\u53ef\u4ee5\u88abRNase H\u8bc6\u522b\uff0c\u5bf9\u6742\u4ea4\u94fe\u4e2d\u7684RNA\u8fdb\u884c\u5185\u5207\u3002\u5229\u7528\u8fd9\u4e00\u539f\u7406\u7684ASOs\u79f0\u4e3aRNase H-dependent ASOs. \u8fd9\u4e00\u7c7bASO\u53d1\u6325\u4f5c\u7528\u4e3b\u8981\u9700\u8981\u6ee1\u8db33\u4e2a\u6761\u4ef6\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>High RNA binding affinity<\/li>\n\n\n\n<li>Nuclease resistance<\/li>\n\n\n\n<li>RNase H activation<\/li>\n<\/ul>\n\n\n\n<p>\u5373\uff0c\u5728\u4fdd\u8bc1\u4e0e\u9776RNA\u9ad8\u4eb2\u548c\u529b\u7684\u540c\u65f6\uff0c\u80fd\u591f\u9ad8\u6548\u6fc0\u6d3bRNase H\u7684\u5185\u5207\u6d3b\u6027\uff0c\u540c\u65f6\u4fdd\u8bc1\u81ea\u8eab\u80fd\u591f\u62b5\u6297\u6838\u9178\u9176\u964d\u89e3\uff0c\u4fdd\u6301\u8f83\u957f\u7684\u534a\u8870\u671f\u3002\u4e3a\u4e86\u5b9e\u73b0\u8fd9\u4e9b\u6761\u4ef6\uff0c\u9700\u8981\u5728\u5929\u7136DNA\u7684\u57fa\u7840\u4e0a\u8fdb\u884c\u5316\u5b66\u4fee\u9970\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u786b\u4ee3\u78f7\u9178\u916f\u952e<\/h2>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202307222320524.jpg?x-oss-process=style\/screenshots\" alt=\"img\" \/><\/figure>\n\n\n\n<p>\u7b2c\u4e00\u4ee3ASOs\u5c06\u78f7\u9178\u4e8c\u916f\u952e\u4e0a\u7684\u4e00\u4e2a\u6c27\u539f\u5b50\u66ff\u6362\u4e3a\u786b\u539f\u5b50\uff0c\u5982\u4e0a\u56fe\u3002<\/p>\n\n\n\n<p>\u4f18\u70b9\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5408\u6210\u7b80\u5355<\/li>\n\n\n\n<li>\u589e\u52a0\u4e86nuclease resistance<\/li>\n\n\n\n<li>\u589e\u52a0\u4e86RNase H activation\u80fd\u529b<\/li>\n\n\n\n<li>\u589e\u52a0\u4e86\u88ab\u7ec6\u80deuptake\u7684\u80fd\u529b<\/li>\n<\/ul>\n\n\n\n<p>\u7f3a\u70b9\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u964d\u4f4e\u4e86\u5bf9\u9776RNA\u7684\u4eb2\u548c\u529b\uff08\u6bcf\u4e00\u4e2a\u786b\u4ee3\u964d\u4f4e0.8\u2103\uff09\/\/\u5e94\u8be5\u6307\u7684\u662f\u6742\u4ea4\u94fe\u9000\u706b\u6e29\u5ea6<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">2&#8242;-O-MOE\u4fee\u9970<\/h2>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202307222321705.jpg?x-oss-process=style\/screenshots\" alt=\"img\" \/><\/figure>\n\n\n\n<p>\u5982\u4e0a\u56fe\u6240\u793a\uff0c2\u2019C\u540e\u9762\u8fde1\u4e2a\u6c27\u539f\u5b50\uff0c\u518d\u63a5\u4e00\u4e2a\u70f7\u57fa\u662f\u5e38\u89c1\u7684\u4fee\u9970\u65b9\u5f0f\uff0c\u78b3\u94fe\u8d8a\u957f\uff0cnuclease resistance\u8d8a\u5f3a\uff0c\u4f46\u540c\u65f6RNA binding affinity\u53d8\u5f31\u3002\u7965\u9e3f\u5728\u552e\u7684\u662f\u5176\u4e2d\u76842\u2019-O-methoxyethyl\uff082\u2019-O-MOE\uff09\u4fee\u9970\u3002<\/p>\n\n\n\n<p>\u4f18\u70b9\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u4f7fASO\u6784\u8c61\u66f4\u63a5\u8fd1RNA\uff0c\u4ece\u800c\u6781\u5927\u589e\u5f3aASO\u7684\u5bf9target RNA\u7684binding affinity.<\/li>\n<\/ul>\n\n\n\n<p>\u7f3a\u70b9\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u65e0\u6cd5\u6fc0\u6d3bRNase H\uff08\u56e0\u4e3a\u6742\u4ea4\u94fe\u6784\u8c61\u66f4\u63a5\u8fd1RNA-RNA hybrids\u800c\u975eRNase H\u9700\u8981\u7684DNA-RNA hybrids\uff09<\/li>\n<\/ul>\n\n\n\n<p>\u4e3a\u4e86\u5229\u75282\u2019-O-MOE\u589e\u5f3aRNA binding affinity\u7684\u4f18\u70b9\uff0c\u540c\u65f6\u7ed5\u8fc7\u5b83\u65e0\u6cd5\u6fc0\u6d3bRNase H\u7684\u7f3a\u70b9\uff0c\u73b0\u5728\u901a\u5e38\u53ea\u5728\u4e24\u6bb5\u51e0\u4e2abases\u52a02\u2019-O-MOE\u4fee\u9970\uff08wings\uff09\uff0c\u4e2d\u95f4\u75596~10\u4e2abases\u4f5c\u4e3aRNase H\u5207\u5272\u4f4d\u70b9\uff08gap\uff09\uff0c\u79f0\u4e3a\u201dgapmer\u201d\u6280\u672f\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">FANA<\/h2>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202307222321684.jpg?x-oss-process=style\/screenshots\" alt=\"img\" \/><\/figure>\n\n\n\n<p>FANA\u5168\u79f0\u662f2\u2019-Deoxy-2\u2019-fluroarabino nucleic acid\uff0c\uff082\u2019-\u8131\u6c27-2\u2019-\u6c1f\u963f\u62c9\u4f2f\u6838\u9178\uff09\u3002\u8981\u6ce8\u610f\u5b83\u4e0d\u540c\u4e8e\u76f4\u63a5\u5728\u8131\u6c27\u6838\u7cd6\u6838\u82f7\u91782\u2019\u78b3\u539f\u5b50\u4e0a\u76842\u2019-F\u4fee\u9970\uff0c\u56e0\u4e3aANA\u548cRNA\u76842\u2019C\u4e0a\u6c22\u539f\u5b50\u548c\u7f9f\u57fa\u7684\u65b9\u5411\u4e0d\u540c\uff0c\u89c1\u4e0b\u56fe\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202307222321192.jpg?x-oss-process=style\/screenshots\" alt=\"img\" \/><\/figure>\n\n\n\n<p>\u4f18\u70b9\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u6bd42\u2019-O-MOE\u548cANA\u66f4\u9ad8\u7684RNA binding affinity<\/li>\n\n\n\n<li>\u80fd\u591f\u6fc0\u6d3bRNase H\u6d3b\u6027\uff08FANA\u548cANA\u90fd\u53ef\u4ee5\uff09<\/li>\n\n\n\n<li>\u65e0\u9700\u8f6c\u67d3\u8bd5\u5242\uff0c\u81ea\u8eab\u53ef\u8fdb\u5165\u7ec6\u80de<\/li>\n<\/ul>\n\n\n\n<p>\u7f3a\u70b9\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u76f8\u6bd4\u4e8e\u78f7\u9178\u4e8c\u916f\u952e\u7684DNA\u5e76\u4e0d\u589e\u52a0nuclease resistance<\/li>\n<\/ul>\n\n\n\n<p>\u7531\u4e8e2\u2019-F\u4fee\u9970\u672c\u8eab\u4e0d\u589e\u52a0nuclease resistance\uff0c\u6240\u4ee5\u9aa8\u67b6\u4e5f\u9700\u8981\u786b\u4ee3\u78f7\u9178\u916f\u952e\u6765\u589e\u52a0nuclease resistance\uff0c\u5373phosphorothioate-FANA (PS-FANA). \u7136\u800c\uff0c\u5728FANA\u4e2d\u5f15\u5165\u786b\u4ee3\u78f7\u9178\u916f\u952e\u4f1a\u5728\u4e00\u5b9a\u7a0b\u5ea6\u4e0a\u964d\u4f4eRNase H activation\u80fd\u529b\u3002\u6240\u4ee5\uff0cFANA\u5728\u88ab\u7528\u4e8eASOs\u4e5f\u88ab\u8bbe\u8ba1\u6210PS-(FANA-DNA-FANA) chimeric gapmers\u6216Altimers\u7684\u5f62\u5f0f\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202307222321400.jpg?x-oss-process=style\/screenshots\" alt=\"img\" \/><\/figure>\n\n\n\n<p>\u4f46\u5728FANA\u7684gapmer\u4e2d\u7684gap\u4e0d\u50cf2\u2019-modification\u90a3\u6837\u9700\u89816~10\u4e2abases\uff0c\u201dAs few as 2 noncontiguous DNA monomers were sufficient for potent RNase H mediated ASO activity\u201d.<\/p>\n\n\n\n<h1 class=\"wp-block-heading\">Morpholino<\/h1>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" decoding=\"async\" src=\"https:\/\/eamonimgbed.oss-cn-beijing.aliyuncs.com\/img\/202307222321463.jpg?x-oss-process=style\/screenshots\" alt=\"img\" \/><\/figure>\n\n\n\n<p>\u6700\u53f3\u8fb9\u7684\u662fMorpholino<\/p>\n\n\n\n<p>Morpholino\u7684\u7ed3\u6784\u8ddf\u4e0a\u9762\u7684ASOs\u7684\u7ed3\u6784\u5dee\u5f02\u8f83\u5927\uff0c\u7528\u516d\u5143\u7684\u5417\u5549\u73af\u4ee3\u66ff\u4e86\u4e94\u5143\u6838\u7cd6\u73af\uff0c\u540c\u65f6\u7528\u7535\u4e2d\u6027\u7684\u7c7b\u78f7\u9178\u916f\u952e\u4ee3\u66ff\u78f7\u9178\u4e8c\u916f\u952e\u3002\u5b83\u5728\u751f\u7269\u7cfb\u7edf\u4e2d\u975e\u5e38\u7a33\u5b9a\uff0c\u5bf9\u86cb\u767d\u9176\u3001\u916f\u9176\u548c\u6838\u9178\u9176\u90fd\u5177\u6709\u6297\u6027\u3002\u56e0\u4e3a\u5b83\u7684\u7a33\u5b9a\u548c\u5b89\u5168\uff0c\u53ef\u4ee5\u4f7f\u7528\u9ad8\u4e8e2\u2019-O-MOE\u7684\u5242\u91cf\uff0c\u4f46\u5728\u4e00\u9879\u5c0f\u9f20\u5b9e\u9a8c\u4e2d\uff0c\u6ce8\u5c04\u540c\u7b49\u5242\u91cf\u7684morpholino\u6548\u679c\u4e0d\u59822\u2019-O-MOE.<\/p>\n\n\n\n<p>Morpholino\u65e0\u6cd5\u6fc0\u6d3bRNase H\uff0c\u5b83\u53d1\u6325Knockdown\u4f5c\u7528\u4e3b\u8981\u4f9d\u8d56\u4e8e\u7ed3\u5408\u5728\u9776RNA\u7684\u6838\u7cd6\u4f53\u8bc6\u522b\u4f4d\u70b9\u6216\u526a\u63a5\u8bc6\u522b\u4f4d\u70b9\u6291\u5236\u7ffb\u8bd1\u6216\u526a\u63a5\u3002<\/p>\n\n\n\n<p>Morpholino\u662f\u7535\u4e2d\u6027\u7684\uff0c\u6240\u4ee5\u9700\u8981\u7279\u6b8a\u7684\u8f6c\u67d3\u65b9\u6cd5\uff0c\u5176\u4e2d\u4e4b\u4e00\u662f\u5076\u8054\u5e26\u7535\u6c28\u57fa\u9178\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u672c\u6587\u6bd4\u8f83\u4e86\u4e24\u79cd\u53cd\u4e49\u5be1\u6838\u82f7\u9178\uff08ASO\uff09\u6280\u672f\uff1aFANA\u4e0eMorpholino\u3002FANA\u5c5e\u4e8eRNase H\u4f9d\u8d56\u6027ASO\uff0c\u901a\u8fc7\u6fc0\u6d3bRNase H\u964d\u89e3\u9776RNA\uff1b\u800cMorpholino\u4e0d\u4f9d\u8d56RNase H\uff0c\u4e3b\u8981\u901a\u8fc7\u7a7a\u95f4\u4f4d\u963b\u6291\u5236\u7ffb\u8bd1\u548c\u526a\u63a5\u3002\u6587\u7ae0\u8be6\u7ec6\u89e3\u6790\u4e86\u4e24\u8005\u7684\u5316\u5b66\u4fee\u9970\u3001\u4f5c\u7528\u673a\u5236\u53ca\u5404\u81ea\u7684\u4f18\u7f3a\u70b9\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,4],"tags":[],"class_list":["post-205","post","type-post","status-publish","format-standard","hentry","category-uncategorized","category-note"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/205","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=205"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/205\/revisions"}],"predecessor-version":[{"id":14155,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/205\/revisions\/14155"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=205"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=205"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=205"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}