{"id":211,"date":"2023-08-07T02:32:58","date_gmt":"2023-08-06T18:32:58","guid":{"rendered":"http:\/\/39.105.184.3\/?p=211"},"modified":"2025-12-21T01:06:20","modified_gmt":"2025-12-20T17:06:20","slug":"20230813bw-life-report","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=211","title":{"rendered":"\u5468\u8bb05"},"content":{"rendered":"\n

7.31-8.6<\/p>\n\n\n\n

\u90fd\u6ca1\u60f3\u8d77\u6765\u5199\u3002<\/p>\n\n\n\n

\u8fd9\u51e0\u5929\u5b9e\u9a8c\u5ba4\u53ea\u6709\u5149\u8001\u5e08\u3001\u5f20\u548f\u598d\u3001\u6211\u548c\u8001\u677f\u3002MRZ\u7684\u6587\u7ae0\u7ec8\u4e8e\u8981\u6295\u51fa\u53bb\u4e86\uff0c\u7ed9\u6211\u53d1\u4e86Manuscript\uff0c\u770b\u6587\u98ce\u662fScience\uff0c\u51e0\u4e2a\u6708\u524d\u6211\u8bf4\u8fc7\u4e0d\u8981\u6302\u6211\u7684\u540d\u5b57\uff0c\u6700\u540e\u8fd8\u662f\u6302\u4e0a\u4e86\u3002<\/p>\n\n\n\n

8\u67085\u53f7\u53bb\u542c\u4e86\u6731\u8001\u5e08\u8bb2\u4e86\u4e00\u4e0b\u6587\u7ae0\u5185\u5bb9\uff0c\u91cd\u70b9\u653e\u5728ZNF512\u548cZNF512B\u901a\u8fc7non-consecutive\u7684TTC\u5b9e\u73b0\u5728\u4e0d\u540c\u7269\u79cd\uff08\u7ed3\u5408\u5e8f\u5217\u5dee\u5f02\u5927\uff09\u4e2d\u7684\u529f\u80fd\u4fdd\u5b88\u578b\uff0c\u90fd\u80fd\u62db\u52dfSuv39h1\u548cSuv39h2\u5b9e\u73b0pericentromeric hetero-chromatin \u7684\u5efa\u7acb\u3002<\/p>\n\n\n\n

\u6211\u8fd9\u51e0\u5929\u4e3b\u8981\u5728\u505a\uff1a<\/p>\n\n\n\n

    \n
  1. PX320-GFP-sg1~10\u5305\u75c5\u6bd2<\/li>\n\n\n\n
  2. Pb-Cas13d-U6N-mCherry\u8d28\u7c92\u6784\u5efaC1\\C6\u4e0d\u6210\u529f\u7684\u95ee\u9898\uff0c\u8d28\u7c92\u4e2d\u4e24\u4e2a\u5e8f\u5217\u76f8\u540c\u7684SV40 polyA site\u4e4b\u95f4\u7684\u533a\u57df\u975e\u5e38\u5bb9\u6613\u4e22\u5931\uff0c\u5143\u8f7d\u4f53\u6362\u6210\u4e86bGH polyA signal\u3002<\/li>\n\n\n\n
  3. Des-V5-MCP-APEX2-IRES-BSD\u7684\u7a33\u8f6c\u3001\u7136\u540e\u5355\u514b\u9686\u6253\u677f\u3002<\/li>\n\n\n\n
  4. Overexpression\u4e86HA-RTCB\u548cHA-ASW\u7684N1,N2,C1,C6\u7a33\u8f6c\u7ec6\u80de\u7cfb\u6784\u5efa\u3002\u9700\u8981\u4e86\u52a0dox\u8bf1\u5bfcCas13d\u8868\u8fbe\u540eC1\u548cC6\u5927\u91cf\u6b7b\u4ea1\u7684\u60c5\u51b5\uff0c\u4e4b\u540e\u53ef\u80fd\u91cd\u65b0\u6784\u5efa\u4e86\u3002dox\u6d53\u5ea6\u964d\u4f4e\u3002<\/li>\n<\/ol>\n\n\n\n

    \u9f99\u65b0\u3001\u5b87\u6cfd\u3001\u96e8\u6db5\u8fd4\u6821\u4e86\uff0c\u4e07\u5764\u8d70\u4e86\u3002<\/p>\n\n\n\n

    8.7<\/p>\n\n\n\n

    \u65e9\u4e0a\u9192\u5f97\u5f88\u65e9\uff0c\u4e0d\u5230\u516b\u70b9\u5230\u4e86\u5b9e\u9a8c\u5ba4\uff0c\u6574\u7406\u4e86\u4e00\u4e0b\u7b2c\u4e94\u5468\u6218\u62a5\u3002\u7136\u540e\u5341\u70b9\u949f\u53bb\u65b0\u6c34\u5229\u9986\u627e\u4e86\u6768\u8001\u5e08\uff0c\u7ea6\u4e86\u660e\u5929\u4e0b\u5348\u4e00\u70b9\u53bb\u6e05\u6cb3\u7ba1\u7406\u5904\u8c08\u6c34\u57df\u3002\u4e0b\u5348\u505a\u4e86\u6d41\u5f0f\u6253\u677fMCP-APEX2\uff0c\u771f\u5feb\u3002\u5341\u5206\u949f\u5c31\u5206\u597d\u4e863\u4e2a96\u5b54\u677f\uff0c\u636e\u8bf4\u662f\u6211\u7684\u7ec6\u80de\u6ca1\u6709\u8367\u5149\u6240\u4ee5\u5feb\u3002\u7136\u540e\u63d0\u4e86\u5f88\u591a\u5f88\u591a\u8d28\u7c92\u3002\u610f\u5916\u5730\u53d1\u73b0polyA change\u7684Cas13d-mCherry\u6ca1\u6709\u957f\u8d77\u6765\u3002\u6536\u4e86PX320-GFP\u768448h\u75c5\u6bd2\u3002\u53bb\u627e\u5b87\u6cfd\u53bb31\u53f7\u697c\u7ec3\u4e86\u51e0\u7ec4\u80f8\u548c\u4e8c\u5934\u3002\u7136\u540e\u53bb\u9f99\u8239\u7801\u5934\u5403\u4e86\u706b\u9505\u3002\u804a\u4e86\u5f88\u591a\u8bfe\u9898\u4e0a\u7684\u4e8b\u60c5\u3002\u975e\u5e38\u503c\u5f97\u6211\u6ce8\u610f\u7684\u662f\uff0c\u5b87\u6cfd\u63d0\u9192\u6211L1RNA\u7684\u4e8c\u7ea7\u7ed3\u6784\u53ef\u80fd\u662f\u88ab\u7279\u5f02\u8bc6\u522b\u7684\u539f\u56e0\u3002\u8fd8\u6709\u6709\u6ca1\u6709\u53ef\u80fd\u53ea\u662f\u56e0\u4e3aL1 RNA\u7279\u522b\u957f\u6240\u4ee5\u88ab\u68c0\u6d4b\u5230\u4e86ligation\uff0c\u5176\u4ed6\u7684\u5c0fRNA\u90fd\u88ab\u8d28\u63a7\u8fc7\u6ee4\u6389\u4e86\u3002\u8ddf\u5b87\u6cfd\u4ea4\u6d41\u4e86\u4e00\u4e0b\uff0c\u6211\u53d1\u73b0\u81ea\u5df1\u5bf9\u8bfe\u9898\u80cc\u666f\u7684\u4e86\u89e3\u8fd8\u5dee\u5f97\u8fdc\u3002<\/p>\n\n\n\n

    8.8~8.13<\/p>\n\n\n\n

    \u8bb2\u5b8c\u4e86\u7ec4\u4f1a\u3002\u7136\u540e\u5468\u516d\u65e5\u7ed9\u81ea\u5df1\u653e\u4e86\u4e24\u5929\u5047\u3002\u5468\u4e09\u5468\u56db\u4e5f\u57fa\u672c\u6ca1\u505a\u5b9e\u9a8c\uff0c\u672c\u6765\u662f\u7559\u51fa\u6765\u7ed9\u7ec4\u4f1a\u7684\uff0c\u4f46\u662f\u5b9e\u9645\u4e0a\u90fd\u5728\u5bbf\u820d\u5e8a\u4e0a\u9893\u8fc7\u53bb\u4e86\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

    \u8fd9\u7bc7\u5468\u8bb0\u8bb0\u5f55\u4e86\u4e3b\u4eba\u5728\u5b9e\u9a8c\u5ba4\u7684\u65e5\u5e38\uff1a\u4eceMRZ\u6587\u7ae0\u5373\u5c06\u6295\u9012Science\u7684\u8fdb\u5c55\uff0c\u5230\u8046\u542c\u5173\u4e8eZNF\u86cb\u767d\u5bb6\u65cf\u529f\u80fd\u4fdd\u5b88\u6027\u7684\u5b66\u672f\u62a5\u544a\u3002\u5b9e\u9a8c\u65b9\u9762\u6d89\u53ca\u75c5\u6bd2\u5305\u88c5\u3001\u8d28\u7c92\u6784\u5efa\u4f18\u5316\u3001\u7a33\u8f6c\u7ec6\u80de\u7cfb\u5efa\u7acb\u4e0e\u6d41\u5f0f\u5206\u9009\u3002\u4e3b\u4eba\u4e5f\u53cd\u601d\u4e86\u4e0e\u540c\u95e8\u4ea4\u6d41\u540e\u5bf9\u8bfe\u9898\u80cc\u666f\u8ba4\u77e5\u7684\u4e0d\u8db3\uff0c\u4ee5\u53ca\u7a7f\u63d2\u5176\u95f4\u7684\u7ec4\u4f1a\u4e0e\u77ed\u6682\u4f11\u6574\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"class_list":["post-211","post","type-post","status-publish","format-standard","hentry","category-blog"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/211","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=211"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/211\/revisions"}],"predecessor-version":[{"id":14154,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/211\/revisions\/14154"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=211"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=211"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=211"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}