{"id":3168,"date":"2024-04-29T14:59:03","date_gmt":"2024-04-29T06:59:03","guid":{"rendered":"https:\/\/fanyiming.life\/?p=3168"},"modified":"2025-12-21T01:05:10","modified_gmt":"2025-12-20T17:05:10","slug":"line-1-fish-with-if","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=3168","title":{"rendered":"LINE-1 FISH with IF"},"content":{"rendered":"\n<p><a href=\"https:\/\/journals.plos.org\/plosgenetics\/article?id=10.1371\/journal.pgen.1001150#pgen.1001150-Dombroski1\">Characterization of LINE-1 Ribonucleoprotein Particles | PLOS Genetics<\/a><\/p>\n\n\n\n<p>\u6ca1\u6709\u901a\u8bfb\u8fd9\u7bc7\u6587\u7ae0\u3002<\/p>\n\n\n\n<p>\u6536\u85cf\u7684\u7406\u7531\u5728\u4e8e\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u8fd9\u7bc7\u6587\u7ae0\u5c06LINE-1 RNA\u76843&#8217;UTR\u5076\u805424\u4e2aMS2 stem loop\u7684\u4e32\u8054\u91cd\u590d\uff0c\u7136\u540e\u7528\u5e26\u8367\u5149\u7684MS2-Cy3 probe\u5bf9LINE-1 RNA\u505aFISH\uff0c\u540c\u65f6\u8fd8\u505aanti-TAP(\u5076\u8054orf2p protein)\u7684IF\uff0c\u5176\u4e2d\u7684protocol\u503c\u5f97\u501f\u9274\u3002<\/li>\n\n\n\n<li>\u6587\u7ae0\u63d0\u5230\uff0c\u7528\u5b9e\u9a8c\u8bc1\u660e\u4e8624\u4e2aMS2 stem loops\u4e0d\u4f1a\u5bf9LINE-1\u7684\u8f6c\u5ea7\u6548\u7387\u4ea7\u751f\u5f71\u54cd\uff0c\u4e0e\u6211\u7684\u8bfe\u9898\u76f8\u5173\u3002\u6211\u53ea\u5728LINE-1\u540e\u9762\u8ddf\u4e864\u4e2aMS2 stem loops\uff0c\u5982\u679c24\u4e2a\u4e0d\u4f1a\u4ea7\u751f\u5f71\u54cd\uff0c\u90a3\u4e48\u5927\u6982\u73874\u4e2a\u66f4\u4e0d\u4f1a\u5f71\u54cdLINE-1 function\uff0c\u540c\u65f6\uff0c\u8fd8\u56e0\u4e3a\u7f29\u77ed\u4e86RNA\u957f\u5ea6\u4ece\u800c\u51cf\u5c11\u4e86\u9274\u5b9a\u5230\u4e86LINE-1 RNA interacting proteins\u7684\u80cc\u666f\u3002<\/li>\n<\/ol>\n\n\n\n<p>\u6211\u5df2\u7ecf\u7528RIP\u5b9e\u9a8c\u8bc1\u660e\u4e86\uff0cMCP-APEX2\u80fd\u591f\u5bcc\u96c64x MS2\u3002\u518d\u52a0\u4e00\u4e2aLINE-1 FISH with anti-V5(MCP-APEX2)\u7684IF\u7684\u5171\u5b9a\u4f4d\u5b9e\u9a8c\uff0c\u80fd\u591f\u66f4\u52a0\u5145\u5206\u3002<\/p>\n\n\n\n<p>\u4e5f\u53ef\u4ee5\u505a\u4e00\u505a\u8fd9\u7bc7\u6587\u732e\u4e2d\u63d0\u5230\u7684\u8f6c\u5ea7\u6548\u7387\u9a8c\u8bc1\u7684\u5b9e\u9a8c\uff0c\u5230\u65f6\u5019\u653e\u5728supplementaries\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd9\u7bc7PLOS Genetics\u6587\u732e\u4e3aLINE-1 RNA\u7684\u6d3b\u7ec6\u80de\u6210\u50cf\u63d0\u4f9b\u4e86\u7ecf\u5178\u65b9\u6cd5\uff1a\u901a\u8fc7MS2-MCP\u7cfb\u7edf\u8fdb\u884c\u5355\u5206\u5b50RNA FISH\uff0c\u5e76\u4e0eORF2p\u86cb\u767d\u7684\u514d\u75ab\u8367\u5149\u5171\u5b9a\u4f4d\u3002\u5176\u5173\u952e\u9a8c\u8bc1\u8868\u660e\uff0c\u5f15\u516524x MS2\u830e\u73af\u7ed3\u6784\u4e0d\u5f71\u54cdLINE-1\u7684\u8f6c\u5ea7\u6d3b\u6027\uff0c\u4e3a\u76f8\u5173\u63a2\u9488\u8bbe\u8ba1\u63d0\u4f9b\u4e86\u91cd\u8981\u4f9d\u636e\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[21],"class_list":["post-3168","post","type-post","status-publish","format-standard","hentry","category-18","tag-line-1"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/3168","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3168"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/3168\/revisions"}],"predecessor-version":[{"id":14012,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/3168\/revisions\/14012"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3168"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3168"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3168"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}