{"id":3763,"date":"2024-05-16T06:17:14","date_gmt":"2024-05-15T22:17:14","guid":{"rendered":"https:\/\/fanyiming.life\/?p=3763"},"modified":"2025-12-21T01:05:00","modified_gmt":"2025-12-20T17:05:00","slug":"%e9%85%b6%e5%88%87%e4%ba%a7%e7%89%a9%e8%87%aa%e8%bf%9e","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=3763","title":{"rendered":"\u9176\u5207\u4ea7\u7269\u81ea\u8fde"},"content":{"rendered":"\n<p>\u6458\u8981\uff1a\u8bbe\u8ba1\u6784\u514b\u9686\u7684\u65f6\u5019\u7a81\u7136\u60f3\u5230\u8fd9\u4e2a\u4ee5\u524d\u6ca1\u8003\u8651\u8fc7\u7684\u95ee\u9898\u3002\u5982\u679c\u53ea\u6709\u4e00\u4e2a\u5355\u4e00\u7684\u5177\u6709\u7c98\u6027\u672b\u7aef\u7684\u5e8f\u5217\uff0c\u5b83\u662f\u5426\u4f1a\u5728DNA\u8fde\u63a5\u9176\u5b58\u5728\u7684\u6761\u4ef6\u4e0b\u5f62\u6210\u591a\u805a\u4f53\uff1f\u505a\u4e86\u4e00\u4e2a\u7b80\u5355\u7684\u5b9e\u9a8c\uff0c\u53d1\u73b0\u786e\u5b9e\u4f1a\u81ea\u8fde\uff0c\u800c\u4e14\u6982\u7387\u5e76\u4e0d\u4f4e\uff0c\u5982\u679c\u5145\u5206\u53cd\u5e94\uff0c\u8d85\u8fc750%\u7684\u7247\u6bb5\u4f1a\u5f62\u6210\u81f3\u5c11\u4e8c\u805a\u5316\uff0c\u90e8\u5206\u751a\u81f3\u4e09\u805a\u5316\u3001\u56db\u805a\u5316\u2026\u2026\u5982\u4e0b\u56fe\u6240\u793a\uff1a<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"824\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516151944656-1024x824.jpg?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-3804\" style=\"width:484px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516151944656-1024x824.jpg?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516151944656-300x241.jpg?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516151944656-768x618.jpg?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516151944656.jpg?x-oss-process=style\/default 1453w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>\u5f62\u6210\u591a\u805a\u4f53\u7684\u539f\u56e0\u662f\uff0c\u5927\u90e8\u5206\u5e38\u7528\u7684\u9650\u5236\u6027\u5185\u5207\u9176\u7684\u9176\u5207\u8bc6\u522b\u4f4d\u70b9\u662f\u56de\u6587\u5e8f\u5217\uff0c\u6240\u4ee5\u5f62\u6210\u7684\u7c98\u6027\u672b\u7aef\uff08\u4e00\u822c\u662f4nt\uff09\u53ef\u4ee5\u548c\u81ea\u8eab\u53cd\u5411\u4e92\u8865\u3002\u6bd4\u5982\u4e0a\u56fe\u4e2d\u7684SpeI\u7684\u7c98\u6027\u672b\u7aef\u662f5&#8242;-ctag-3&#8217;\uff0cAgeI\u7684\u7c98\u6027\u672b\u7aef\u662f5&#8242;-ccgg-3&#8217;\uff0c\u90fd\u662f\u56de\u6587\u5e8f\u5217\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5982\u679c\u4e24\u7aef\u90fd\u662f\u56de\u6587\u7c98\u6027\u672b\u7aef\uff0c\u4f1a\u4e32\u8054\u5f62\u6210\u4e8c\u805a\u4f53\u3001\u4e09\u805a\u4f53\u3001\u56db\u805a\u4f53\u2026\u2026<\/li>\n\n\n\n<li>\u5982\u679c\u53ea\u6709\u4e00\u7aef\u662f\u56de\u6587\u5e8f\u5217\u7684\u7c98\u6027\u672b\u7aef\uff0c\u53e6\u4e00\u7aef\u662f\u975e\u56de\u6587\u7c98\u6027\u672b\u7aef\u6216\u5e73\u672b\u7aef\uff0c\u5c31\u53ea\u80fd\u5934\u5bf9\u5934\u5f62\u6210\u4e8c\u805a\u4f53\uff1b<\/li>\n\n\n\n<li>\u5982\u679c\u4e24\u7aef\u90fd\u6ca1\u6709\u56de\u6587\u5e8f\u5217\uff0c\u5c31\u4e0d\u4f1a\u53d1\u751f\u81ea\u8fde<\/li>\n<\/ul>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\" \/>\n\n\n\n<p>\u4e0b\u9762\u662f\u4e0a\u56fe\u7684\u5b9e\u9a8c\u7ec6\u8282<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-ad2f72ca wp-block-group-is-layout-flex\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"87\" height=\"257\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516042241298.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-3767\" style=\"width:104px;height:auto\" \/><\/figure>\n\n\n\n<p>\u75282xM5 hiper plus taq\u901a\u8fc7PCR\u5f97\u5230316bp\u7247\u6bb5\uff0c\u4e0a\u4e0b\u6e38\u5206\u522b\u542b\u6709SpeI\u548cAgeI\u9176\u5207\u8bc6\u522b\u4f4d\u70b9\u3002PCR\u4f53\u7cfb400\u03bcL\uff0c\u7a0b\u5e8f\u7ed3\u675f\u540e\u53d65\u03bcL\u8dd1\u80f6\uff0c\u6761\u5e26\u6b63\u786e\u3002<\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>PCR\u53cd\u5e94\u6db2\u7528\u8bd5\u5242\u76d2\u56de\u6536\u3001\u6d4b\u6d53\u5ea6\u300277.2ng\/\u03bcL\uff0c\u53d634\u03bcL\u4e8e\u65b0\u7684PCR\u7ba1\uff0c\u52a05\u03bcL 10x FastDigest Buffer, 2\u03bcL FastDigest SpeI\u548c2\u03bcL FastDigest AgeI\uff0c\u88657\u03bcL ddH2O\u81f350\u03bcL\u4f53\u7cfb\uff0c37\u2103\u9176\u520715min\u3002<\/p>\n\n\n\n<p>\u9176\u5207\u7ed3\u675f\u540e\uff0c\u53cd\u5e94\u6db2\u7528\u8bd5\u5242\u76d2\u56de\u6536\u3001\u6d4b\u6d53\u5ea6\uff0c\u5f97\u5230101.2ng\/\u03bcL * 17\u03bcL.<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>\u8fd9\u91cc\u53ef\u4ee5\u7a0d\u4f5c\u8ba1\u7b97\uff0c\u9176\u5207\u4ea7\u7269\u56de\u6536\u540e\u4e00\u5171\u6709101.2ng\/\u03bcL * 17\u03bcL = 1720.4 ng\uff0c\u6295\u5165\u7684DNA\u5e95\u7269\u7684\u91cf\u4e3a77.2ng\/\u03bcL * 35\u03bcL = 2702ng\uff0c\u56de\u6536\u6548\u7387\u5927\u6982\u662f1720.4 \/ 2702 = 63.7%. \u5047\u8bbe\u4ecePCR\u53cd\u5e94\u6db2\u4e2d\u56de\u6536\u7684\u6548\u7387\u76f8\u540c\uff0c\u90a3\u4e48PCR\u53cd\u5e94\u6db2\u4e2d\u7684\u76ee\u7684\u7247\u6bb5DNA\u603b\u91cf\u4e3a2702 \/ 63.7% = 4241.8ng\uff0c\u6d53\u5ea6\u4e3a4241.8ng \/ 400\u03bcL = 10.6ng\/\u03bcL. PCR\u6837\u54c1\u8dd1\u80f6\u65f6\uff0c\u4e0a\u6837\u4e865\u03bcL\uff0c\u5c31\u662f 10.6ng\/\u03bcL * 5\u03bcL = 53ng. \u6240\u4ee553ng\u8db3\u4ee5\u5728\u4e0a\u56fe\u7684\u5c0f\u5b541.5% Agarose Gel\u4e0a\u5f97\u5230\u8f83\u4e3a\u6e05\u6670\u7684\u6761\u5e26\u3002<\/p>\n\n\n\n<p>\u800c\u6700\u540e\u7684\u8fde\u63a5\u4f53\u7cfb\u4e2d\uff0cFragment1\u6295\u5165\u4e86101.2ng\/\u03bcL * 4\u03bcL = 404.8ng\uff0c\u5168\u90e8\u4e0a\u6837\u53ef\u80fd\u6761\u5e26\u8fc7\u7c97\u4e0d\u6613\u5206\u8fa8\u5927\u5c0f\uff0c\u800c\u4e0a\u4e00\u6bb5\u53ef\u80fd\u9ad8\u4f30\u8bd5\u5242\u76d2\u80f6\u56de\u6536\u6548\u7387\uff0c\u800c\u4e14\u7ed3\u679c\u53ef\u80fd\u5e76\u4e0d\u662f\u5355\u4e00\u6761\u5e26\uff0c\u6240\u4ee5\u4e5f\u4e0d\u80fd\u4e0a\u6837\u592a\u4f4e\u3002\u7efc\u5408\u8003\u8651\u53ef\u4ee5\u4e0a\u6837250ng\uff0c\u5373250ng \/ 404.8ng * 25\u03bcL = 15.4\u03bcL.<\/p>\n\n\n\n<p>\u7531\u4e8e\u9700\u8981\u5728\u4e0a\u6837\u524d\u52a0\u5341\u5206\u4e4b\u4e00\u768410x loading buffer\uff0c\u6240\u4ee5\u6700\u540e\u9700\u8981\u4e0a\u683715.4 \/ 0.9 = 17.1\u03bcL\uff0c\u4e0d\u8fc7\u6700\u540e\u5b9e\u9645\u4e0a\u6837\u65f6\uff0c\u53d1\u73b0\uff0c\u90a3\u4e2a\u5b54\u88c5\u4e0d\u4e0b17.1\u03bcL\uff0c\u6bcf\u4e2a\u90fd\u6f0f\u4e86\u5927\u69823\u03bcL\uff0c\u53ef\u80fd\u9020\u6210\u4ea4\u53c9\u6c61\u67d3\u3002<\/p>\n<\/blockquote>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>\u7ec4\u522b<\/td><td>Fragment1(SpeI&amp;AgeI\u7c98\u6027\u672b\u7aef\uff08101.2ng\/\u03bcl\uff0c\u7ea6306bp\uff09<\/td><td>Fragment2(AgeI &amp; SphI\u7c98\u6027\u672b\u7aef\uff0820.2ng\/\u03bcl\uff0c\u7ea6501bp\uff09<\/td><td>2xligation buffer (C671A)<\/td><td>T4 DNA ligase (M180A)<\/td><td>ddH2O<\/td><td>\u603b\u4f53\u79ef<\/td><\/tr><tr><td>1<\/td><td>4\u03bcL<\/td><td>&#8211;<\/td><td>12.5\u03bcL<\/td><td>&#8211;<\/td><td>8.5\u03bcL<\/td><td>25\u03bcL<\/td><\/tr><tr><td>2<\/td><td>4\u03bcL<\/td><td>&#8211;<\/td><td>12.5\u03bcL<\/td><td>1\u03bcL<\/td><td>7.5\u03bcL<\/td><td>25\u03bcL<\/td><\/tr><tr><td>3<\/td><td>4\u03bcL<\/td><td>7.5\u03bcL<\/td><td>12.5\u03bcL<\/td><td>&#8211;<\/td><td>1\u03bcL<\/td><td>25\u03bcL<\/td><\/tr><tr><td>4<\/td><td>4\u03bcL<\/td><td>7.5\u03bcL<\/td><td>12.5\u03bcL<\/td><td>1\u03bcL<\/td><td><\/td><td>25\u03bcL<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\">\u8fde\u63a5\u4f53\u7cfb<\/figcaption><\/figure>\n\n\n\n<p>25\u2103\u53cd\u5e9475min<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"881\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516061708180-1024x881.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-3780\" style=\"width:370px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516061708180-1024x881.png?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516061708180-300x258.png?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516061708180-768x661.png?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516061708180-1536x1321.png?x-oss-process=style\/default 1536w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516061708180.png?x-oss-process=style\/default 1615w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>\u770b\u6837\u5b50\u662fFragment2\u7684\u6d53\u5ea6\u592a\u4f4e\u4e86\uff0c\u5bfc\u81f4\u5b83\u7684\u6761\u5e26\u6839\u672c\u770b\u4e0d\u89c1\u3002\u6240\u4ee5\u7ec41\u548c\u7ec43\u3001\u7ec42\u548c\u7ec44\u7684\u6761\u5e26\u7c7b\u578b\u4e00\u6a21\u4e00\u6837\u3002\u4f46\u4ecd\u7136\u80fd\u591f\u770b\u51fa\uff0c\u7ec42\u5355\u7247\u6bb5\u53d1\u751f\u4e86\u81ea\u8fde\uff0c\u800c\u4e14\u8fd9\u79cd\u81ea\u8fde\u662f\u53ef\u4ee5\u53d1\u751f\u4e0d\u6b62\u4e00\u6b21\u7684\uff0c\u751f\u6210\u4e32\u8054\u7684\u6761\u5e26\u3002\u9664\u4e86306bp\u7684\u6574\u500d\u6570\u6761\u5e26\uff0c\u4e2d\u95f4\u8fd8\u5939\u6742\u4e86\u4e00\u4e9b\u5fae\u5f31\u6761\u5e26\uff0c\u53ef\u80fd\u662f\u73af\u72b6DNA\u3002<\/p>\n\n\n\n<p>\u884d\u751f\u5047\u8bbe\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5355\u9176\u5207\u8f7d\u4f53\u548c\u53cc\u9176\u5207\u8f7d\u4f53\uff0c\u90fd\u4f1a\u53d1\u751f\u81ea\u8fde\u3002\u5982\u679c\u9009\u62e9\u975e\u5e38\u9760\u8fd1\u7684\u4e24\u4e2a\u9176\u8fdb\u884c\u5355\u9176\u5207\u6216\u53cc\u9176\u5207\uff0c\u518d\u5c06\u9176\u5207\u4ea7\u7269\u7528T4 ligase\u8fde\u63a5\uff0c\u4ea7\u7269\u6761\u5e26\u5e94\u8be5\u5b8c\u5168\u76f8\u540c\u3002\uff08\u5728\u7c98\u6027\u672b\u7aef\u6c22\u952e\u6570\u91cf\u76f8\u540c\u7684\u60c5\u51b5\u4e0b\uff09\uff1b<\/li>\n\n\n\n<li>\u975e\u56de\u6587\u5e8f\u5217\u7684\u9ecf\u6027\u672b\u7aef\u4e0d\u4f1a\u53d1\u751f\u81ea\u8fde\uff1b<\/li>\n\n\n\n<li>\u5982\u679c\u7247\u6bb5\u7684\u4e00\u7aef\u4e3a\u56de\u6587\u5e8f\u5217\u7c98\u6027\u672b\u7aef\uff0c\u53e6\u4e00\u7aef\u4e3a\u5e73\u672b\u7aef\u6216\u975e\u56de\u6587\u5e8f\u5217\u9ecf\u6027\u672b\u7aef\uff0c\u5c06\u53ea\u80fd\u53d1\u751f\u4e00\u6b21\u81ea\u8fde\u3002\u53ea\u5f97\u5230\u4e24\u500d\u5927\u5c0f\u7684\u8fde\u63a5\u4ea7\u7269\uff0c\u6ca1\u67093\u500d\u30014\u500d\u2026\u2026\u6216\u6210\u73af\u7684\u4ea7\u7269\u3002<\/li>\n<\/ol>\n\n\n\n<p>\u5bf9\u4e8e\u6784\u5efa\u514b\u9686\u7684\u6307\u5bfc\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5c3d\u91cf\u4e0d\u8981\u8fdb\u884c\u8d85\u8fc72\u4e2a\u7247\u6bb5\u7684\u8fde\u63a5\uff0c\u6bcf\u591a\u4e00\u4e2a\u7247\u6bb5\uff0c\u8fde\u63a5\u6548\u7387\u90fd\u4f1a\u65ad\u5d16\u5f0f\u4e0b\u964d\uff1b<\/li>\n\n\n\n<li>\u8fdb\u884c2\u4e2a\u7247\u6bb5\u4ee5\u4e0a\u7684\u8fde\u63a5\u65f6\uff0c\u8bf4\u660e\u4e66\u4e0a\u7684\u7247\u6bb5\u5927\u5c0f\u548c\u6469\u5c14\u6bd4\u8981\u4e25\u683c\u9075\u5b88\u3002<\/li>\n\n\n\n<li>\u4e00\u822c\u6765\u8bb2\uff0cT4 ligase\u7684\u5546\u4e1a\u8bf4\u660e\u4e66\u4f1a\u5efa\u8bae\uff0c\u8d8a\u5c0f\u7684\u7247\u6bb5\u7684\u6469\u5c14\u6570\u8981\u8d8a\u9ad8\u3002\u6211\u4ee5\u524d\u7406\u89e3\u5b83\u7684\u539f\u56e0\u662f\u201cT4 ligase\u4f1a\u4f18\u5148\u7ed3\u5408\u5927\u7247\u6bb5\u201d\uff0c\u4f46\u73b0\u5728\u770b\u6765\u539f\u56e0\u4e5f\u8bb8\u4e0d\u662f\u8fd9\u6837\uff0c\u5c0f\u7247\u6bb5\u7684\u5206\u5b50\u81ea\u7531\u78b0\u649e\u6982\u7387\u66f4\u9ad8\uff0c\u5e94\u8be5\u88abT4 ligase\u7ed3\u5408\u6982\u7387\u66f4\u5927\u624d\u5bf9\u3002\u4f46\u8fd9\u4e2a\u6469\u5c14\u6bd4\u7684\u8003\u8651\u6211\u8fd8\u6ca1\u6709\u60f3\u6e05\u695a\u3002<\/li>\n<\/ol>\n\n\n\n<p>\u8fd8\u662f\u4e0a\u9762\u7684306bp\u7247\u6bb5\uff0c\u5982\u679c\u53ea\u5355\u9176\u5207\u7684\u8bdd\uff0c\u56e0\u4e3a\u53ea\u6709\u4e00\u4fa7\u6709\u53cd\u5411\u4e92\u8865\u7684\u56de\u6587\u7c98\u6027\u672b\u7aef\uff0c\u6240\u4ee5\u53ea\u5f62\u6210\u201c\u4e8c\u805a\u4f53\u201d\uff0c\u5982\u4e0b\u56fe(2% Agarose\u80f6)\uff1a<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"824\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516151944656-1024x824.jpg?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-3804\" style=\"width:550px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516151944656-1024x824.jpg?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516151944656-300x241.jpg?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516151944656-768x618.jpg?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/05\/20240516151944656.jpg?x-oss-process=style\/default 1453w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd9\u7bc7\u7b14\u8bb0\u63a2\u8ba8\u4e86\u5206\u5b50\u514b\u9686\u4e2d\u4e00\u4e2a\u5e38\u88ab\u5ffd\u7565\u7684\u73b0\u8c61\uff1a\u5f53DNA\u7247\u6bb5\u4e24\u7aef\u5177\u6709\u56de\u6587\u5e8f\u5217\u7684\u7c98\u6027\u672b\u7aef\u65f6\uff0c\u5728\u8fde\u63a5\u9176\u4f5c\u7528\u4e0b\u4f1a\u53d1\u751f\u663e\u8457\u7684\u81ea\u8fde\uff0c\u5f62\u6210\u591a\u805a\u4f53\u3002\u4f5c\u8005\u901a\u8fc7\u4e25\u8c28\u7684\u5b9e\u9a8c\u9a8c\u8bc1\u4e86\u8fd9\u4e00\u70b9\uff0c\u5e76\u5206\u6790\u4e86\u4e0d\u540c\u672b\u7aef\u7c7b\u578b\u5bf9\u81ea\u8fde\u7ed3\u679c\u7684\u5f71\u54cd\uff0c\u4e3a\u514b\u9686\u5b9e\u9a8c\u8bbe\u8ba1\u63d0\u4f9b\u4e86\u91cd\u8981\u53c2\u8003\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-3763","post","type-post","status-publish","format-standard","hentry","category-note"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/3763","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3763"}],"version-history":[{"count":3,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/3763\/revisions"}],"predecessor-version":[{"id":13993,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/3763\/revisions\/13993"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3763"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3763"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3763"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}