{"id":4535,"date":"2024-06-25T23:09:58","date_gmt":"2024-06-25T15:09:58","guid":{"rendered":"https:\/\/fanyiming.life\/?p=4535"},"modified":"2025-12-21T01:04:55","modified_gmt":"2025-12-20T17:04:55","slug":"saf-a%e5%92%8cnuclear-rna%e5%bd%a2%e6%88%90%e7%bd%91%e7%bb%9c%e8%b0%83%e6%8e%a7%e8%bd%ac%e5%bd%95","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=4535","title":{"rendered":"SAF-A\u548cnuclear RNA\u5f62\u6210\u7f51\u7edc\u8c03\u63a7\u8f6c\u5f55"},"content":{"rendered":"\n<p><a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2024.06.16.599208v1.full.pdf\">Nuclear RNA forms an interconnected network of transcription-dependent and tunable microgels (biorxiv.org)<\/a><\/p>\n\n\n\n<p>\u79d1\u5b66\u95ee\u9898\uff1a\u7ec6\u80de\u6838\u5185\u7684\u5fae\u73af\u5883\u662f\u600e\u6837\u7684<\/p>\n\n\n\n<p>\u63d0\u51fa\u5047\u8bbe\uff1aSAF-A\u548cnuclear RNA\u5f62\u6210\u4e00\u79cd\u80f6\u72b6\u7f51\u7edc\u7ed3\u6784\uff0c\u6587\u4e2d\u6210\u4e3a&#8221;microgel&#8221;\u3002microgel\u5185\u90e8\u86cb\u767d\u8d28\u7684\u6269\u6563\u901f\u7387\u66f4\u4f4e\uff0c\u800cmicrogel\u591a\u7684\u5730\u65b9\u7684\u67d3\u8272\u8d28\u66f4\u677e\u6563\u3001\u8f6c\u5f55\u66f4\u6d3b\u8dc3\u3002microgel\u672c\u8eab\u53ef\u4ee5\u88ab\u4e00\u79cd\u6838\u9178\u5185\u5207\u9176XRN2\u964d\u89e3\u3002<\/p>\n\n\n\n<p>\u4e3b\u8981\u5b9e\u9a8c\uff1a<\/p>\n\n\n\n<p>1\uff09\u901a\u8fc7\u8d85\u5206\u8fa8\u663e\u5fae\u955c\u89c2\u5bdf\u5230SAF-A\u548cnuclear RNA\u5f62\u6210microgel\u7ed3\u6784<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"708\" height=\"208\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232047342.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-4538\" style=\"width:840px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232047342.png?x-oss-process=style\/default 708w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232047342-300x88.png?x-oss-process=style\/default 300w\" sizes=\"auto, (max-width: 708px) 100vw, 708px\" \/><\/figure>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>2\uff09\u786e\u5b9a\u4e86SAF-A\u4e2d\u8ddfRNA\u76f8\u4e92\u4f5c\u7528\u7684\u7ed3\u6784\u57df<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"720\" height=\"218\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232215253.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-4539\" style=\"width:730px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232215253.png?x-oss-process=style\/default 720w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232215253-300x91.png?x-oss-process=style\/default 300w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/figure>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>3\uff09KD SAF-A\u6216\u6291\u5236\u8f6c\u5f55\u540emicrogel\u51cf\u5c11\u4e86<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"750\" height=\"474\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232437979.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-4540\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232437979.png?x-oss-process=style\/default 750w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232437979-300x190.png?x-oss-process=style\/default 300w\" sizes=\"auto, (max-width: 750px) 100vw, 750px\" \/><\/figure>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>4\uff09\u4f34\u968f\u7740\u4e0a\u56fe\u4e2dmicrogel\u7684\u51cf\u5c11\uff0c\u6838\u5185\u86cb\u767d\u8d28\u7684\u6269\u6563\u6548\u7387\u589e\u5f3a\u4e86<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"760\" height=\"317\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232855341.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-4541\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232855341.png?x-oss-process=style\/default 760w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625232855341-300x125.png?x-oss-process=style\/default 300w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><\/figure>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>5\uff09microgel\u672c\u8eab\u53ef\u4ee5\u88ab\u4e00\u79cd\u6838\u9178\u5185\u5207\u9176XRN2\u964d\u89e3<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"652\" height=\"251\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625233323407.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-4542\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625233323407.png?x-oss-process=style\/default 652w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240625233323407-300x115.png?x-oss-process=style\/default 300w\" sizes=\"auto, (max-width: 652px) 100vw, 652px\" \/><\/figure>\n\n\n\n<p>\u56feG\u5bf9\u4ea4\u8054\u540e\u5bf9SAF-A\u505aWB\uff0c\u5f53\u03b1-amanitin\u6291\u5236\u8f6c\u5f55\uff0c\u540c\u65f6\u5b58\u5728XRN2\u65f6\uff0cSAF-A\u66f4\u591a\u662fmonomer.<\/p>\n\n\n\n<p>\u6700\u540e\u8fd8\u6709\u4e00\u4e9bfigure\u7814\u7a76\u4e86micro\u5bf9\u67d3\u8272\u8d28\u201c\u53bb\u538b\u7f29\u201d\u7684\u529f\u80fd\u3002<\/p>\n\n\n\n<p>\u603b\u7ed3\uff1a\u672c\u6587\u7814\u7a76\u63ed\u793a\u4e86RNA\u548cSAF-A\u76f8\u4e92\u4f5c\u7528\u5f62\u6210\u7684\u5fae\u51dd\u80f6\u5728\u7ec6\u80de\u6838\u5185\u7684\u91cd\u8981\u4f5c\u7528\u3002\u901a\u8fc7\u9ad8\u5206\u8fa8\u7387\u6210\u50cf\u548c\u5206\u5b50\u52a8\u529b\u5b66\u6a21\u62df\uff0c\u4f5c\u8005\u53d1\u73b0\u8fd9\u4e9b\u5fae\u51dd\u80f6\u4fc3\u8fdb\u67d3\u8272\u8d28\u53bb\u538b\u7f29\uff0c\u4ece\u800c\u8c03\u63a7\u57fa\u56e0\u8868\u8fbe\u3002\u8fd9\u4e00\u673a\u5236\u901a\u8fc7RNA\u964d\u89e3\u9176XRN2\u7684\u8c03\u63a7\u8fdb\u4e00\u6b65\u5f97\u5230\u4e86\u9a8c\u8bc1\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p>\u4e00\u4e9bQ&amp;A.<\/p>\n\n\n\n<p>1\uff09\u76ee\u5149\u600e\u4e48\u8f6c\u79fb\u5230XRN2\u4e0a\u7684\uff1f<\/p>\n\n\n\n<p>\u7b54\uff1a\u901a\u8fc7BioID\u6280\u672f\u53d1\u73b0XRN2\u662fSAF-A\u7684proximal proteins. \u7ed3\u5408XRN2\u662f\u4e00\u79cd\u6838\u9178\u5185\u5207\u9176\uff0c\u4e8e\u662f\u8054\u60f3\u5230\u5b83\u662f\u5426\u80fd\u901a\u8fc7\u964d\u89e3nuclear RNA\u6765\u8c03\u63a7microgel.<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>We reasoned that proteins important for RNA turnover were likely to be spatially located in the vicinity of the nucleoplasmic network and microgels, so BioID74 (proximity-dependent biotin identification) was used to identify proteins in the vicinity of the SAF-A, by tagging the protein on the N-terminus with BirA. As expected, SAF-A was observed to be in close proximity to its homologues HNRNPUL1 and HNRNPUL2 and other members of a nuclear scaffold: SAFB, SAFB2 and MATRIN3 (Fig S4A; Table S1), but, surprisingly, 5&#8242;-3&#8242; Exoribonuclease 2 (XRN2) was also enriched.<\/p>\n<\/blockquote>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd9\u7bc7\u9884\u5370\u672c\u7814\u7a76\u63ed\u793a\u4e86\u7ec6\u80de\u6838\u5185\u8f6c\u5f55\u8c03\u63a7\u7684\u65b0\u7269\u7406\u673a\u5236\u3002\u4f5c\u8005\u53d1\u73b0SAF-A\u86cb\u767d\u4e0e\u6838\u5185RNA\u901a\u8fc7\u76f8\u4e92\u4f5c\u7528\uff0c\u5f62\u6210\u4e86\u4e00\u79cd\u53ef\u8c03\u63a7\u7684\u201c\u5fae\u51dd\u80f6\u201d\u7f51\u7edc\u3002\u8fd9\u79cd\u7ed3\u6784\u4e0d\u4ec5\u5f71\u54cd\u6838\u5185\u86cb\u767d\u6269\u6563\uff0c\u8fd8\u4e0e\u67d3\u8272\u8d28\u72b6\u6001\u53ca\u8f6c\u5f55\u6d3b\u6027\u5bc6\u5207\u76f8\u5173\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[19],"class_list":["post-4535","post","type-post","status-publish","format-standard","hentry","category-18","tag-rna"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4535","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4535"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4535\/revisions"}],"predecessor-version":[{"id":13982,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4535\/revisions\/13982"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4535"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4535"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4535"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}