{"id":4622,"date":"2024-06-27T01:37:06","date_gmt":"2024-06-26T17:37:06","guid":{"rendered":"https:\/\/fanyiming.life\/?p=4622"},"modified":"2025-12-21T01:04:52","modified_gmt":"2025-12-20T17:04:52","slug":"nuclear-actin%e6%88%90%e5%83%8f","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=4622","title":{"rendered":"nuclear actin\u6210\u50cf"},"content":{"rendered":"\n<p>[ez-toc]<\/p>\n\n\n\n<p>\u4e00\u5171\u627e\u52304\u79cd\u5bf9nuclear actin\u6210\u50cf\u7684\u5b9e\u9a8c\u3002\u524d3\u79cd\u90fd\u662f\u6d3b\u7ec6\u80de\u6210\u50cf\uff0c\u5c06\u5177\u6709actin-binding\u80fd\u529b\u7684\u591a\u80bd\u878d\u5408GFP\u548cNLS\uff0c\u7f3a\u70b9\u662f\u53ef\u80fd\u63a2\u9488\u672c\u8eab\u4f1a\u5f71\u54cdactin\u529f\u80fd\u3002\u7b2c4\u79cd\u662f\u56fa\u5b9a\u7ec6\u80de\u7528\u9b3c\u7b14\u73af\u80bd\u6807\u8bb0\uff0c\u96be\u70b9\u5728nuclear filament actins\u7684\u91cf\u8fdc\u5c0f\u4e8ecytoplasmic filament actins.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">R1-EN<\/h3>\n\n\n\n<p>\u662fRPEL1\u7ed3\u6784\u57df(actin-binding)\u4e0eEGFP-NLS\u878d\u5408\u5f62\u6210\u7684\u63a2\u9488\uff0c\u7528\u4e8e\u6807\u8bb0\u548c\u89c2\u5bdf\u6838\u5185<strong>\u5355\u4f53<\/strong>\u808c\u52a8\u86cb\u767d<\/p>\n\n\n\n<p>\u5f62\u6001\uff1a\u4e00\u90e8\u5206\u5728nucleoli\u5bcc\u96c6\uff0c\u5176\u4ed6\u7684\u5728nucleoplasm\u70b9\u72b6\u5206\u5e03\uff0c\u4e0e&#8221;nuclear speckles&#8221;\u5171\u5b9a\u4f4d<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"475\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627044750699-1024x475.jpg?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-4630\" style=\"width:773px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627044750699-1024x475.jpg?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627044750699-300x139.jpg?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627044750699-768x357.jpg?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627044750699-1536x713.jpg?x-oss-process=style\/default 1536w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627044750699-2048x951.jpg?x-oss-process=style\/default 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23447706\/\">Visualization of actin filaments and monomers in somatic cell nuclei &#8211; PubMed (nih.gov)<\/a><\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Utr230-EN<\/h3>\n\n\n\n<p>Utrophin(actin-binding)\u7684\u622a\u77ed\u4f53\u4e0eEGFP-NLS\u878d\u5408\u5f62\u6210\u7684\u63a2\u9488\uff0c\u7528\u4e8e\u6807\u8bb0\u548c\u89c2\u5bdf\u6838\u5185<strong>\u7ea4\u7ef4\u72b6<\/strong>\u808c\u52a8\u86cb\u767d\u3002<\/p>\n\n\n\n<p>\u5f62\u6001\uff1a\u7ec6\u80de\u6838\u5185\u70b9\u72b6\u5206\u5e03\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"373\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627045744281-1024x373.jpg?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-4632\" style=\"width:855px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627045744281-1024x373.jpg?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627045744281-300x109.jpg?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627045744281-768x280.jpg?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627045744281-1536x559.jpg?x-oss-process=style\/default 1536w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627045744281-2048x746.jpg?x-oss-process=style\/default 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23447706\/\">Visualization of actin filaments and monomers in somatic cell nuclei &#8211; PubMed (nih.gov)<\/a><\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">LifeAct-GFP-NLS<\/h3>\n\n\n\n<p>\u9175\u6bcdAbp140\u86cb\u767d\u6765\u6e90\u7684\u77ed\u80bd(LifeAct)\u4e0eGFP-NLS\u878d\u5408\u5f62\u6210\u7684\u63a2\u9488\uff0c\u7ed3\u5408<strong>\u5355\u4f53\u548c\u7ea4\u7ef4\u72b6<\/strong>\u808c\u52a8\u86cb\u767d\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"725\" height=\"985\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627031048303.jpg?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-4626\" style=\"width:404px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627031048303.jpg?x-oss-process=style\/default 725w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627031048303-221x300.jpg?x-oss-process=style\/default 221w\" sizes=\"auto, (max-width: 725px) 100vw, 725px\" \/><\/figure>\n\n\n\n<p>\u5f62\u6001\uff1a\u5728serum-starved\u7684\u7ec6\u80de\u6838\u4e2d, LifeAct-GFP-NLS\u5448\u5f25\u6563\u5206\u5e03(A)\u300220% serum\u523a\u6fc0\u540e, \u572815s\u5185\u5f62\u6210\u7ea4\u7ef4\u7f51\u72b6\u7ed3\u6784\u3002<\/p>\n\n\n\n<p><a href=\"https:\/\/www.science.org\/doi\/10.1126\/science.1235038?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub%20%200pubmed\">Nuclear Actin Network Assembly by Formins Regulates the SRF Coactivator MAL | Science<\/a><\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">&nbsp;dye-conjugated phalloidin<\/h3>\n\n\n\n<p>phalloidin\u662f\u7ecf\u5178\u7684\u5bf9\u56fa\u5b9a\u7ec6\u80de\u4e2d<strong>\u7ea4\u7ef4\u72b6<\/strong>\u808c\u52a8\u86cb\u767d\uff08\u5927\u4e8e7\u4e2amonomer actins\u4e32\u8054\uff09\u6807\u8bb0\u7684\u591a\u80bd\u63a2\u9488\u3002\u4e0b\u56fe\u662f\u4eba\u4e3a\u53bb\u6389\u4e86\u5927\u90e8\u5206\u7ec6\u80de\u8d28\u540e\u7684\u6837\u672c\uff0c\u51cf\u5c11\u7ec6\u80de\u8d28\u4e2dF-actins\u7684\u5e72\u6270\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"465\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627051636843-1024x465.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-4636\" style=\"width:730px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627051636843-1024x465.png?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627051636843-300x136.png?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627051636843-768x349.png?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627051636843.png?x-oss-process=style\/default 1090w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>\u5f62\u6001\uff1a\u4e00\u90e8\u5206\u5bcc\u96c6\u5728nucleoli\uff0c\u5176\u4ed6\u5757\u72b6\u5206\u5e03\u3002<\/p>\n\n\n\n<p>\u5982\u679c\u4e0d\u53bb\u9664\u7ec6\u80de\u8d28\u76f4\u63a5\u7528phalloidin\u6807\u8bb0\u7684\u8bdd\uff0c\u5f88\u96be\u5728nucleus\u4e2d\u770b\u5230\u4fe1\u53f7\uff0c\u5927\u90e8\u5206\u90fd\u662f\u7ec6\u80de\u8d28\u4e2d\u7684\u8367\u5149\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"533\" height=\"897\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627054108471.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-4641\" style=\"width:555px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627054108471.png?x-oss-process=style\/default 533w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627054108471-178x300.png?x-oss-process=style\/default 178w\" sizes=\"auto, (max-width: 533px) 100vw, 533px\" \/><\/figure>\n\n\n\n<p>G13R\u548cR62D\u662f\u7834\u574ffilament\u5f62\u6210\u7684dominant-negative mutant\uff0c\u52a0\u5165\u6838\u4fe1\u53f7\u540e\u8fc7\u8868\u8fbe\u8fd9\u6837\u7684mutant\u4f7fnuclear actins\u7684\u7ea4\u7ef4\u7ed3\u6784\u88ab\u7834\u574f\uff0cphalloidin\u4fe1\u53f7\u5bc6\u5ea6\u51cf\u5f31\u3002\u800cS14C\u662f\u589e\u5f3afilaments\u5f62\u6210\u7684mutant\uff0c\u4e0a\u56feB.<\/p>\n\n\n\n<p><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7159918\/\">Nuclear actin regulates inducible transcription by enhancing RNA polymerase II clustering &#8211; PMC (nih.gov)<\/a><\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u4e00\u79cd&#8221;KD&#8221;nuclear actin\u7684\u65b9\u6cd5\u662f\uff1a\u8fc7\u8868\u8fbe\u8d1f\u8d23\u5c06nuclear actins\u4ece\u6838\u5b54\u8fd0\u51fa\u7684\u86cb\u767dXPO6\uff0c\u4ece\u800c\u7279\u5f02\u6027\u964d\u4f4enuclear actins\u6c34\u5e73\u3002\u76f8\u6bd4\u4e8e\u76f4\u63a5\u5bf9\u03b2-actin\u7684\u57fa\u56e0\u8868\u8fbe\u8fdb\u884c\u64cd\u7eb5\uff0c\u51cf\u5c11\u4e86cytoplasmic actins\u529f\u80fd\u53d7\u635f\u7684\u98ce\u9669\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"387\" height=\"178\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627054932493.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-4643\" style=\"width:557px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627054932493.png?x-oss-process=style\/default 387w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/06\/20240627054932493-300x138.png?x-oss-process=style\/default 300w\" sizes=\"auto, (max-width: 387px) 100vw, 387px\" \/><\/figure>\n\n\n\n<p>\u4e0a\u56fe\u4e0a\u8fb9\u7684\u7ec6\u80de\u662f\u8fc7\u8868\u8fbeXPO6\u7684\uff0c\u6838\u5185YFP\u660e\u663e\u4f4e\u4e8e\u4e0b\u9762\u7684\u7ec6\u80de\u3002<\/p>\n\n\n\n<p><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7159918\/\">Nuclear actin regulates inducible transcription by enhancing RNA polymerase II clustering &#8211; PMC (nih.gov)<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u672c\u6587\u7efc\u8ff0\u4e86\u56db\u79cd\u6838\u5185\u808c\u52a8\u86cb\u767d\u6210\u50cf\u65b9\u6cd5\uff0c\u6db5\u76d6\u6d3b\u7ec6\u80de\u63a2\u9488\uff08R1-EN\u3001Utr230-EN\u3001LifeAct-GFP-NLS\uff09\u4e0e\u56fa\u5b9a\u7ec6\u80de\u67d3\u8272\uff08\u9b3c\u7b14\u73af\u80bd\uff09\uff0c\u5e76\u5206\u6790\u4e86\u5404\u81ea\u7684\u6280\u672f\u539f\u7406\u3001\u5e94\u7528\u573a\u666f\u4e0e\u6f5c\u5728\u5c40\u9650\u6027\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-4622","post","type-post","status-publish","format-standard","hentry","category-18"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4622","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4622"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4622\/revisions"}],"predecessor-version":[{"id":13977,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4622\/revisions\/13977"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4622"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4622"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4622"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}