{"id":4669,"date":"2024-07-02T13:57:32","date_gmt":"2024-07-02T05:57:32","guid":{"rendered":"https:\/\/fanyiming.life\/?p=4669"},"modified":"2025-12-21T01:04:51","modified_gmt":"2025-12-20T17:04:51","slug":"arp2-3%e8%b0%83%e6%8e%a7%e8%bd%ac%e5%bd%95","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=4669","title":{"rendered":"Arp2\/3\u8c03\u63a7\u8f6c\u5f55"},"content":{"rendered":"\n

Yoo Y, Wu X, Guan JL. A novel role of the actin-nucleating Arp2\/3 complex in the regulation of RNA polymerase II-dependent transcription.<\/a> J Biol Chem. 2007 Mar 9;282(10):7616-23. doi: 10.1074\/jbc.M607596200. Epub 2007 Jan 12. PMID: 17220302.<\/p>\n\n\n\n

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\u6587\u732e\u603b\u7ed3<\/h3>\n\n\n\n

\u7814\u7a76\u76ee\u7684<\/h4>\n\n\n\n

\u672c\u7814\u7a76\u65e8\u5728\u63a2\u7d22actin-nucleating Arp2\/3 complex\u5728RNA\u805a\u5408\u9176II\u4f9d\u8d56\u7684\u8f6c\u5f55\u4e2d\u7684\u65b0\u4f5c\u7528\uff0c\u5c24\u5176\u662f\u5176\u5728\u7ec6\u80de\u6838\u4e2d\u7684\u5b9a\u4f4d\u53ca\u5176\u901a\u8fc7N-WASP\u4ecb\u5bfc\u7684\u808c\u52a8\u86cb\u767d\u805a\u5408\u5bf9\u8f6c\u5f55\u8c03\u63a7\u7684\u5f71\u54cd\u3002<\/p>\n\n\n\n

\u80cc\u666f<\/h4>\n\n\n\n
    \n
  • Actin\u5df2\u77e5\u5b58\u5728\u4e8e\u7ec6\u80de\u6838\u4e2d\uff0c\u5e76\u53c2\u4e0e\u5305\u62ec\u8f6c\u5f55\u8c03\u63a7\u5728\u5185\u7684\u591a\u79cd\u6838\u529f\u80fd\u3002<\/li>\n\n\n\n
  • Arp2\/3 complex\u5728\u7ec6\u80de\u8d28\u4e2d\u7684\u4f5c\u7528\u5df2\u88ab\u5e7f\u6cdb\u7814\u7a76\uff0c\u5176\u4e3b\u8981\u901a\u8fc7WASP\u5bb6\u65cf\u86cb\u767d\u6fc0\u6d3b\uff0c\u4fc3\u8fdb\u808c\u52a8\u86cb\u767d\u7684\u6210\u6838\u548c\u5206\u652f\u5f62\u6210\u3002<\/li>\n\n\n\n
  • \u8fd1\u671f\u7814\u7a76\u8868\u660eN-WASP\u80fd\u591f\u5728\u7ec6\u80de\u6838\u5185\u8bf1\u5bfc\u808c\u52a8\u86cb\u767d\u7684de novo\u805a\u5408\uff0c\u8fd9\u5bf9\u4e8eRNA\u805a\u5408\u9176II\u4f9d\u8d56\u7684\u8f6c\u5f55\u8c03\u63a7\u81f3\u5173\u91cd\u8981\u3002<\/li>\n<\/ul>\n\n\n\n

    \u5b9e\u9a8c\u8bbe\u8ba1\u4e0e\u7ed3\u679c<\/h4>\n\n\n\n
      \n
    1. \u6838\u5b9a\u4f4d\u7814\u7a76<\/strong>\uff1a<\/li>\n<\/ol>\n\n\n\n
        \n
      • \u901a\u8fc7\u514d\u75ab\u8367\u5149\u663e\u5fae\u955c\u548cWestern blotting\u5206\u6790\uff0c\u786e\u5b9aArp2\u548cArp3\u5728\u7ec6\u80de\u6838\u4e2d\u7684\u5b9a\u4f4d\u3002<\/li>\n\n\n\n
      • \u7ed3\u679c\u8868\u660e\uff0cArp2\u548cArp3\u4e0d\u4ec5\u5b58\u5728\u4e8e\u7ec6\u80de\u8d28\u4e2d\uff0c\u8fd8\u5728\u7ec6\u80de\u6838\u4e2d\u6709\u663e\u8457\u5b9a\u4f4d\u3002<\/li>\n<\/ul>\n\n\n\n
          \n
        1. siRNA\u4ecb\u5bfc\u7684\u86cb\u767d\u8d28\u6572\u4f4e<\/strong>\uff1a<\/li>\n<\/ol>\n\n\n\n
            \n
          • \u4f7f\u7528\u7279\u5f02\u6027siRNA\u6572\u4f4eHeLa\u7ec6\u80de\u4e2d\u7684Arp2\u548cArp3\uff0c\u7ed3\u679c\u663e\u793a\uff0c\u8fd9\u4e9b\u86cb\u767d\u5728\u7ec6\u80de\u6838\u4e2d\u7684\u542b\u91cf\u663e\u8457\u964d\u4f4e\uff0c\u8fdb\u4e00\u6b65\u9a8c\u8bc1\u4e86\u5176\u6838\u5b9a\u4f4d\u3002<\/li>\n<\/ul>\n\n\n\n
              \n
            1. pyrene actin assays<\/strong>\uff1a<\/li>\n<\/ol>\n\n\n\n
                \n
              • \u5728\u4f53\u5916\u5b9e\u9a8c\u4e2d\uff0c\u7814\u7a76\u4e86Arp2\u548cArp3\u5bf9N-WASP\u4ecb\u5bfc\u7684\u808c\u52a8\u86cb\u767d\u805a\u5408\u7684\u5f71\u54cd\u3002<\/li>\n\n\n\n
              • \u7ed3\u679c\u8868\u660e\uff0c\u6572\u4f4eArp2\u6216Arp3\u4f1a\u663e\u8457\u51cf\u5c11\u808c\u52a8\u86cb\u767d\u805a\u5408\uff0c\u800c\u6dfb\u52a0\u7eaf\u5316\u7684Arp2\/3\u590d\u5408\u7269\u53ef\u4ee5\u6062\u590d\u805a\u5408\u6d3b\u6027\u3002<\/li>\n<\/ul>\n\n\n\n
                  \n
                1. \u8f6c\u5f55\u8c03\u63a7\u7814\u7a76<\/strong>\uff1a<\/li>\n<\/ol>\n\n\n\n
                    \n
                  • \u4f7f\u7528BrU\u63ba\u5165\u5b9e\u9a8c\u548c\u4f53\u5916\u8f6c\u5f55\u5b9e\u9a8c\uff0c\u8bc4\u4f30Arp2\u548cArp3\u5728RNA\u805a\u5408\u9176II\u4f9d\u8d56\u8f6c\u5f55\u4e2d\u7684\u4f5c\u7528\u3002<\/li>\n\n\n\n
                  • \u7ed3\u679c\u663e\u793a\uff0c\u6572\u4f4eArp2\u6216Arp3\u663e\u8457\u964d\u4f4e\u4e86\u8f6c\u5f55\u6d3b\u6027\uff0c\u800c\u5177\u6709siRNA\u6297\u6027\u7684Arp2\u548cArp3\u7a81\u53d8\u4f53\u53ef\u4ee5\u6062\u590d\u8f6c\u5f55\u3002<\/li>\n<\/ul>\n\n\n\n

                    \u8ba8\u8bba<\/h4>\n\n\n\n
                      \n
                    • \u672c\u7814\u7a76\u63d0\u4f9b\u4e86\u5f3a\u6709\u529b\u7684\u8bc1\u636e\uff0c\u8868\u660eArp2\/3 complex\u5728\u7ec6\u80de\u6838\u4e2d\u901a\u8fc7\u8c03\u63a7\u808c\u52a8\u86cb\u767d\u805a\u5408\u53c2\u4e0eRNA\u805a\u5408\u9176II\u4f9d\u8d56\u7684\u8f6c\u5f55\u3002<\/li>\n\n\n\n
                    • \u63d0\u51faN-WASP\u548cArp2\/3 complex\u53ef\u80fd\u901a\u8fc7\u7c7b\u4f3c\u4e8e\u5176\u5728\u7ec6\u80de\u8d28\u4e2d\u7684\u673a\u5236\uff0c\u5728\u7ec6\u80de\u6838\u5185\u8c03\u63a7\u8f6c\u5f55\u3002<\/li>\n\n\n\n
                    • \u672a\u6765\u7684\u7814\u7a76\u9700\u8981\u8fdb\u4e00\u6b65\u63a2\u7d22\u6838\u808c\u52a8\u86cb\u767d\u7684\u805a\u5408\u673a\u5236\u53ca\u5176\u5728\u57fa\u56e0\u8f6c\u5f55\u548c\u5176\u4ed6\u6838\u529f\u80fd\u4e2d\u7684\u4f5c\u7528\u3002<\/li>\n<\/ul>\n\n\n\n

                      \u521b\u65b0\u70b9\u4e0e\u4e0d\u8db3<\/h4>\n\n\n\n
                        \n
                      • \u521b\u65b0\u70b9<\/strong>\uff1a\u9996\u6b21\u660e\u786e\u4e86Arp2\/3 complex\u5728\u7ec6\u80de\u6838\u4e2d\u7684\u529f\u80fd\uff0c\u62d3\u5c55\u4e86\u5176\u5df2\u77e5\u7684\u7ec6\u80de\u8d28\u4f5c\u7528\u3002<\/li>\n\n\n\n
                      • \u4e0d\u8db3<\/strong>\uff1a\u4e3b\u8981\u4f9d\u8d56\u4f53\u5916\u548c\u7ec6\u80de\u5b9e\u9a8c\uff0c\u5c1a\u9700\u5728\u4f53\u5185\u7814\u7a76\u4e2d\u9a8c\u8bc1\u8fd9\u4e9b\u53d1\u73b0\u7684\u751f\u7406\u76f8\u5173\u6027\u3002<\/li>\n<\/ul>\n\n\n\n

                        \u8fd9\u7bc7\u6587\u7ae0\u901a\u8fc7\u4e00\u7cfb\u5217\u5b9e\u9a8c\uff0c\u63ed\u793a\u4e86Arp2\/3 complex\u5728\u7ec6\u80de\u6838\u4e2d\u7684\u65b0\u89d2\u8272\u53ca\u5176\u5bf9RNA\u805a\u5408\u9176II\u4f9d\u8d56\u8f6c\u5f55\u7684\u91cd\u8981\u5f71\u54cd\uff0c\u4e3a\u7406\u89e3\u7ec6\u80de\u6838\u5185\u808c\u52a8\u86cb\u767d\u7684\u529f\u80fd\u63d0\u4f9b\u4e86\u65b0\u7684\u89c6\u89d2\u3002<\/p>\nGenerated by ChatGPT 4o<\/cite><\/blockquote>\n\n\n\n

                        <\/div>\n\n\n\n

                        Figure 5\u7684\u5b9e\u9a8c\u5f88\u5de7\u5999\uff0c\u4f5c\u8005\u53d1\u73b0\u7528siRNA KD Arp2\u548cArp3\u540ePol II\u8f6c\u5f55\u4e0b\u964d\u3002\u7136\u800c\uff0c\u8fd9\u79cd\u4e0b\u964d\u662f\u5426\u662f\u7531\u201cnuclear Arp2\/3\u201d\u800c\u975e\u201ccytoplasmic Arp2\/3\u201d\u7684KD\u6240\u5f15\u8d77\u7684\u5462\uff1f\u4e3a\u4e86\u7814\u7a76\u8fd9\u4e00\u70b9\uff0c\u4f5c\u8005\u5f80Arp2\/3\u7684\u540e\u8fb9\u52a0\u4e86\u6838\u8f93\u51fa\u4fe1\u53f7NES (nucleus export signal)\uff0c\u8fd9\u6837\u5916\u6e90\u5bfc\u5165\u7684Arp2\/3-NES\u5c31\u53ea\u5b9a\u4f4d\u5728\u7ec6\u80de\u8d28\u4e2d\uff0c\u540c\u65f6\uff0c\u5f15\u5165\u540c\u4e49\u7a81\u53d8\u4f7f\u5f97siRNA\u53ea\u80fd\u6c89\u9ed8\u5185\u6e90Arp2\/3\uff0c\u800c\u65e0\u6cd5\u6c89\u9ed8\u5916\u6e90\u5bfc\u5165\u7684\u3001\u5b9a\u4f4d\u5728\u7ec6\u80de\u8d28\u7684Arp2\/3-NES\uff0c\u4ece\u800c\u5b9e\u73b0\u4e86\u53eaKD nuclear Arp2\/3\u7684\u5b9e\u9a8c\u8bbe\u8ba1\u3002<\/p>\n\n\n\n

                        \u5728\u6b64\u57fa\u7840\u4e0a\u4f5c\u8005\u8fd8\u8bbe\u7f6e\u4e86\u4e25\u8c28\u7684\u5bf9\u7167\u3002\u901a\u8fc7\u7a81\u53d8\u6539\u53d8NES\u7684\u758f\u6c34\u6027\u6b8b\u57fa\uff08\u5982\u4eae\u6c28\u9178\uff09\uff0c\u4f7f\u5176\u5931\u53bb\u6838\u8f93\u51fa\u529f\u80fd\uff08NESm\uff09\u3002\u76f8\u6bd4\u4e8eArp2\/3-NES\u53ea\u5b9a\u4f4d\u5728\u7ec6\u80de\u8d28\uff0cArp2\/3-NESm\u5c31\u5373\u5b9a\u4f4d\u5728\u7ec6\u80de\u8d28\uff0c\u4e5f\u5b9a\u4f4d\u5728\u7ec6\u80de\u6838\uff0c\u4f5c\u4e3a\u201c\u9634\u6027\u5bf9\u7167\u201d\u3002<\/p>\n\n\n\n

                        <\/div>\n\n\n\n
                        \"\"<\/figure>\n\n\n\n

                        siRNA\u5904\u7406\u5185\u6e90Arp2\/3\uff0c\u5e72\u6270\u4e86Pol II\u4ecb\u5bfc\u7684\u8f6c\u5f55\u3002<\/p>\n\n\n\n

                        <\/div>\n\n\n\n
                        \"\"<\/figure>\n\n\n\n

                        \u5728\u5916\u6e90\u5bfc\u5165\u7684Arp2\/3\u4e2d\u5f15\u5165\u4e0d\u6539\u53d8\u6c28\u57fa\u9178\u5e8f\u5217\u7684\u540c\u4e49\u7a81\u53d8\uff0c\u8fd9\u4e9b\u7a81\u53d8\u540e\u7684Arp2\/3\u514d\u75absiRNA\u3002<\/p>\n\n\n\n

                        <\/div>\n\n\n\n
                        \"\"<\/figure>\n\n\n\n

                        NES\u4fe1\u53f7\u4f7f\u86cb\u767d\u5b9a\u4f4d\u5728\u7ec6\u80de\u8d28\uff0c\u800cNESm\u5728\u6838\u8d28\u5747\u6709\u5b9a\u4f4d\u3002<\/p>\n\n\n\n

                        <\/div>\n\n\n\n
                        \"\"<\/figure>\n\n\n\n

                        \u53ea\u6c89\u9ed8nuclear Arp2\/3\u5c31\u53ef\u4ee5\u5f71\u54cdPol II\u4ecb\u5bfc\u7684\u8f6c\u5f55\uff08lane 3\u548clane6\uff09\u3002<\/p>\n\n\n\n

                        <\/p>\n\n\n\n

                        <\/p>\n","protected":false},"excerpt":{"rendered":"

                        \u8fd9\u7bc72007\u5e74\u7684\u7814\u7a76\u63ed\u793a\u4e86\u808c\u52a8\u86cb\u767d\u6210\u6838\u590d\u5408\u7269Arp2\/3\u5728\u7ec6\u80de\u6838\u4e2d\u7684\u65b0\u529f\u80fd\u3002\u4f5c\u8005\u901a\u8fc7\u7cbe\u5de7\u7684\u5b9e\u9a8c\u8bbe\u8ba1\uff0c\u8bc1\u5b9e\u4e86\u6838\u5185\u7684Arp2\/3\u80fd\u901a\u8fc7N-WASP\u4ecb\u5bfc\u7684\u808c\u52a8\u86cb\u767d\u805a\u5408\uff0c\u8c03\u63a7RNA\u805a\u5408\u9176II\u4f9d\u8d56\u7684\u8f6c\u5f55\uff0c\u4e3a\u7406\u89e3\u6838\u9aa8\u67b6\u4e0e\u57fa\u56e0\u8868\u8fbe\u7684\u5173\u7cfb\u63d0\u4f9b\u4e86\u5173\u952e\u8bc1\u636e\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-4669","post","type-post","status-publish","format-standard","hentry","category-18"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4669","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4669"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4669\/revisions"}],"predecessor-version":[{"id":13976,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4669\/revisions\/13976"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4669"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4669"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4669"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}