{"id":4999,"date":"2024-07-24T01:51:41","date_gmt":"2024-07-23T17:51:41","guid":{"rendered":"https:\/\/fanyiming.life\/?p=4999"},"modified":"2025-12-21T01:04:43","modified_gmt":"2025-12-20T17:04:43","slug":"%e7%b3%bb%e7%bb%9f%e9%89%b4%e5%ae%9adna-transposon","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=4999","title":{"rendered":"\u7cfb\u7edf\u9274\u5b9aDNA transposon"},"content":{"rendered":"\n<p>Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao Y, Chen Q, Guo B, Wang H, Zhang YE. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/38843831\/\">Heterologous survey of 130 DNA transposons in human cells highlights their functional divergence and expands the genome engineering toolbox.<\/a> Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 10.1016\/j.cell.2024.05.007. Epub 2024 Jun 5. PMID: 38843831.<\/p>\n\n\n\n<div style=\"height:45px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u5c06DNA transposon\u62c6\u5206\u6210tranpose enzyme (helper plasmid)\u548cTIR-<strong>G<\/strong>ene<strong>O<\/strong>f<strong>I<\/strong>nterest-TIR (donor plasmid)\u7684\u53cc\u8d28\u7c92\u9012\u9001\u7cfb\u7edf\u5df2\u7ecf\u6210\u4e3a\u6210\u719f\u7684\u57fa\u56e0\u7a33\u5b9a\u8868\u8fbe\u5de5\u5177\uff08\u5982sleeping beauty, Piggybac\uff09\u3002\u4e0d\u8fc7\u73b0\u6709\u7684\u5de5\u5177\u4e0d\u4e00\u5b9a\u662f\u6700\u597d\u7684\uff0c\u56e0\u4e3aDNA transposon\u5148\u524d\u6ca1\u6709\u5927\u89c4\u6a21\u7cfb\u7edf\u6027\u7814\u7a76\uff0c\u4f5c\u8005\u4ece102\u4e2a\u7269\u79cd\u4e2d\u7528\u5e72\u5b9e\u9a8c\u9274\u5b9a\u51fa130\u4e2a\u65b0\u7684DNA transposons\uff0c\u5e0c\u671b\u4ece\u4e2d\u627e\u5230\u66f4\u52a0\u597d\u7528\u7684\u5de5\u5177\u3002 \u5728human cells\u4e2d\u7528\u6e7f\u5b9e\u9a8c\u6d4b\u8bd5\u4e86\u8fd9130\u4e2aDNA transposons\u7684\u8f6c\u5ea7\u6d3b\u6027\uff0c\u53d1\u73b0\u670940\u4e2a\u662f\u6709\u8f6c\u5ea7\u6d3b\u6027\u7684\uff08\u4e4b\u524d\u5df2\u77e5\u7684\u53ea\u670920\u4e2aactive DNA transposons\uff09\u3002<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>TIR, terminal inverted repeats,\u4e00\u4e2a\u901a\u5e38\u7684DNA transposons\u7684\u7ed3\u6784\u662fTIR-transposase-TIR.<\/p>\n<\/blockquote>\n\n\n\n<p>\u4f5c\u8005\u5c1d\u8bd5\u7528\u5b83\u4eec\u9274\u5b9a\u5230\u7684\u65b0\u8f6c\u5ea7\u5b50\u4e2d\u6307\u6807\u6700\u51fa\u8272\u7684&#8221;MAG&#8221;\u8f6c\u5ea7\u5b50\u6765\u8fdb\u884cCAR-T\u7597\u6cd5\u7684\u5e94\u7528\uff0c\u7ed3\u679c\u5e76\u4e0d\u4e9a\u4e8e\u7ecf\u5178\u7684\u5df2\u7ecf\u5f00\u53d1\u8fed\u4ee3\u4e86\u5f88\u591a\u4ee3\u7684\u5de5\u5177Sleeping beauty\u3002\u4f5c\u8005\u8ba4\u4e3a\u201cMAG\u201d\u5728\u4eca\u540e\u5e8f\u5217\u4f18\u5316\u540e\u62e5\u6709\u5f88\u9ad8\u7684\u5e94\u7528\u524d\u666f\u3002<\/p>\n\n\n\n<div style=\"height:45px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u9274\u5b9a\u8f6c\u5ea7\u6d3b\u6027\u7684reporter\u5982\u4e0b\uff1a<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"826\" height=\"635\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/07\/20240724022552158.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-5005\" style=\"width:547px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/07\/20240724022552158.png?x-oss-process=style\/default 826w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/07\/20240724022552158-300x231.png?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/07\/20240724022552158-768x590.png?x-oss-process=style\/default 768w\" sizes=\"auto, (max-width: 826px) 100vw, 826px\" \/><\/figure>\n\n\n\n<p>130\u4e2a\u5019\u9009DNA transposons\uff0c\u6bcf\u4e2a\u90fd\u6709\u81ea\u5df1\u72ec\u7279\u7684Tnp\u548cnon-coding sequences(TSD-TIR-Spacer)\uff0c\u6240\u4ee5\u4e00\u5171\u6784\u5efa\u4e86130\u5bf9\u513fDonor plasmid\uff0bHelper plasmid\u3002\u53cc\u8d28\u7c92\u5171\u8f6c\u67d3\u540e\uff0c\u7528\u542b\u6297\u751f\u7d20(puromycin)\u7684\u57f9\u517b\u57fa\u7a00\u91ca\u5230\u6bcf\u4e2a\u7ec6\u80de\u4e4b\u95f4\u7269\u7406\u4e0a\u9694\u5f00\u3002\u57f9\u517b\u4e00\u6bb5\u65f6\u95f4\u540e\uff0c\u6210\u529f\u8f6c\u5ea7\u7684\u7ec6\u80de\u56e0\u4e3a\u6709\u4e86puroR\u6297\u6027\u53ef\u4ee5\u5f62\u6210colony\uff0c\u800c\u6ca1\u6709\u6210\u529f\u8f6c\u5ea7\u7684\u5c31\u6b7b\u4e86\u3002\u901a\u8fc7\u5bf9colonies\u8ba1\u6570\uff0c\u5bf9\u9634\u6027\u5bf9\u7167\uff08\u53ea\u8f6c\u67d3donor plasmid\uff09\u5c31\u80fd\u53cd\u5e94\u8be5DNA transposon\u662f\u5426\u5728human cells\u5177\u6709\u8f6c\u5ea7\u6d3b\u6027\u3002\u5e76\u4e14\u4e0d\u540c\u7684DNA transposons\u4e4b\u95f4\u53ef\u4ee5\u5bf9transposition activity\u8fdb\u884c\u6bd4\u8f83\u3002<\/p>\n\n\n\n<div style=\"height:45px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>Q. \u5229\u7528DNA transposons\u548cRetrotransposons\u6784\u5efa\u7a33\u8f6c\u7cfb\u7edf\u7684\u4f18\u52a3<\/p>\n\n\n\n<p>A. \u524d\u8005\u662fcut-paste, \u540e\u8005\u662fcopy-paste\uff0c\u5929\u7136\u7684Retrotransposons\u4ece\u8fdb\u5316\u4e0a\u770b\uff0c\u5728\u8ddf\u5bbf\u4e3b\u7684\u535a\u5f08\u4e2d\u53d6\u5f97\u7684\u4f18\u52bf\u8981\u5927\u4e8eDNA transposons (human cell\u4e2d\u4e0d\u5b58\u5728\u4ecd\u7136active\u7684DNA transposons\uff0c\u4f46\u662f\u5b58\u5728active Retrotransposons\u6bd4\u5982LINE-1)\u3002\u7136\u800c\uff0c\u73b0\u5b9e\u5e94\u7528\u5c42\u9762\uff0c\u540e\u8005\u8fdc\u4e0d\u5982\u524d\u8005\u7684\u6280\u672f\u6210\u719f\u3002\u53ef\u80fd\u7684\u539f\u56e0\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Retrotransposons\u7684\u8f6c\u5ea7\u7531\u4e8e\u5b58\u5728RNA intermediate\uff0c\u5bf9\u5bbf\u4e3b\u7684\u4f9d\u8d56\u6027\u53ef\u80fd\u66f4\u9ad8\uff0c\u964d\u4f4e\u4e86\u5f02\u6e90\u6574\u5408\u6548\u7387\uff08\u5f02\u6e90\u7684\u610f\u601d\u662f\u5176\u4ed6\u7269\u79cd\u4e2d\u53d1\u73b0\u7684transposons\u5728human cells\u4e2d\u5229\u7528\uff09<\/li>\n<\/ul>\n\n\n\n<p>\u4f46Retrotransposons\u7684\u5f00\u53d1\u6f5c\u529b\u4e5f\u5f88\u5927\uff0c\u56e0\u4e3a\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u9012\u9001ssRNAs\u7684\u6280\u672f\u8981\u6bd4\u9012\u9001dsDNAs\u66f4\u6210\u719f\u3001\u6548\u7387\u66f4\u9ad8<\/li>\n<\/ul>\n\n\n\n<div style=\"height:45px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>Q. \u4e3a\u4ec0\u4e48\u6709\u4e00\u4e9bDNA transposons\u7684\u6574\u5408\u4f4d\u70b9\u66f4\u503e\u5411\u4e8e\u5f02\u67d3\u8272\u8d28\uff1f<\/p>\n\n\n\n<p>A. \u4ece\u76f4\u89c9\u4e0a\uff0c\u5f02\u67d3\u8272\u8d28\u66f4\u52a0\u5c01\u95ed\uff0c\u4e0d\u5229\u4e8e\u6574\u5408\u3002\u4f46\u4ece\u8fdb\u5316\u89c6\u89d2\u770b\uff0ctransposons\u548chost cells\u662f\u535a\u5f08\u5173\u7cfb\uff0c\u66f4\u9ad8\u7684\u8f6c\u5ea7\u6548\u7387\u901a\u5e38\u610f\u5473\u7740\u66f4\u5bb9\u6613\u88ab\u5bbf\u4e3b\u538b\u5236\u3002\u800c\u5728heterochromatin\u7684transposons\u867d\u7136\u727a\u7272\u4e86\u4e00\u5b9a\u7684\u8f6c\u5ea7\u6d3b\u6027\uff0c\u4f46\u540c\u65f6\u4e5f\u589e\u5f3a\u4e86\u81ea\u8eab\u7684\u5b89\u5168\u6027\uff0c\u6709\u53ef\u80fd\u66f4\u5bb9\u6613\u5728\u8fdb\u5316\u4e2d\u4fdd\u7559\u4e0b\u6765\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u5f02\u67d3\u8272\u8d28\u53ef\u80fd\u662f\u517c\u6027\u5f02\u67d3\u8272\u8d28\uff0c\u5e76\u6ca1\u6709\u5b8c\u5168\u9501\u6b7b\u8f6c\u5ea7\u53d1\u751f\u7684\u53ef\u80fd\u3002<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/38753487\/\">2024\u5e74Cell Report<\/a>\u5df2\u7ecf\u62a5\u9053\u4e86\u5386\u53f2\u4e0a\u7b2c\u4e00\u4e2a\u5229\u7528retrotransposon\u8fdb\u884c\u57fa\u56e0\u5f02\u6e90\u8868\u8fbe\u7684\u5de5\u5177\uff08\u6ca1\u70b9\u5f00\u8bfb\u8fc7\uff09<\/p>\n<\/blockquote>\n\n\n\n<div style=\"height:45px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>Q. \u63d2\u5165\u4f4d\u70b9\u5728\u57fa\u56e0\u7ec4\u7684\u5206\u5e03\u4e0e\u6cbb\u7597\u5b89\u5168\u6027\u7684\u5173\u7cfb<\/p>\n\n\n\n<p>A. \u66f4\u5bb9\u6613\u6574\u5408\u5728\u67d3\u8272\u8d28\u5f00\u653e\u4f4d\u70b9\u7684piggybac\u66f4\u5bb9\u6613\u7834\u574f\u6b63\u5e38\u57fa\u56e0\u7684\u8868\u8fbe\uff0c\u7528\u4e8eCAR-T\u5bf9\u4eba\u6765\u8bf4\u5c31\u4e0d\u5b89\u5168\u3002\u65b0\u53d1\u73b0\u7684MAG\u7684\u63d2\u5165\u4f4d\u70b9\u6bd4piggybac\u66f4\u968f\u673a\uff0c\u56e0\u6b64\u6bd4piggybac\u7684\u5b89\u5168\u6027\u66f4\u9ad8\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd9\u7bc7\u300aCell\u300b\u6587\u732e\u901a\u8fc7\u5927\u89c4\u6a21\u5f02\u6e90\u7b5b\u9009\uff0c\u7cfb\u7edf\u9274\u5b9a\u4e86130\u4e2aDNA\u8f6c\u5ea7\u5b50\u5728\u4eba\u7c7b\u7ec6\u80de\u4e2d\u7684\u529f\u80fd\uff0c\u5c06\u5df2\u77e5\u6709\u6d3b\u6027\u7684\u8f6c\u5ea7\u5b50\u6570\u91cf\u7ffb\u500d\uff0c\u5e76\u4ece\u4e2d\u53d1\u73b0\u4e86\u6027\u80fd\u4f18\u5f02\u7684\u65b0\u5de5\u5177\u201cMAG\u201d\uff0c\u4e3a\u57fa\u56e0\u7ec4\u5de5\u7a0b\u5de5\u5177\u7bb1\u63d0\u4f9b\u4e86\u91cd\u8981\u6269\u5c55\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-4999","post","type-post","status-publish","format-standard","hentry","category-18"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4999","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4999"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4999\/revisions"}],"predecessor-version":[{"id":13959,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/4999\/revisions\/13959"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4999"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4999"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4999"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}