{"id":5407,"date":"2024-08-12T06:53:14","date_gmt":"2024-08-11T22:53:14","guid":{"rendered":"https:\/\/fanyiming.life\/?p=5407"},"modified":"2025-12-21T01:04:40","modified_gmt":"2025-12-20T17:04:40","slug":"%e6%95%b0%e6%8d%ae%e5%bd%92%e4%b8%80%e5%8c%96%e7%9a%84%e6%af%94%e8%be%83%ef%bc%9a%e7%ba%bf%e6%80%a7%e5%bd%92%e4%b8%80%e5%8c%96%e4%b8%8e%e5%b0%ba%e5%af%b8%e5%9b%a0%e5%ad%90%e5%bd%92%e4%b8%80%e5%8c%96","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=5407","title":{"rendered":"\u7ebf\u6027\u5f52\u4e00\u5316\u4e0e\u5c3a\u5bf8\u56e0\u5b50\u5f52\u4e00\u5316"},"content":{"rendered":"\n\n\n
\n

Generated by ChatGPT4o<\/p>\n<\/blockquote>\n\n\n\n

\u5728\u6570\u636e\u79d1\u5b66\u548c\u7edf\u8ba1\u5206\u6790\u4e2d\uff0c\u5f52\u4e00\u5316\u662f\u786e\u4fdd\u6570\u636e\u96c6\u4e4b\u95f4\u53ef\u6bd4\u6027\u548c\u51c6\u786e\u6027\u7684\u5173\u952e\u6b65\u9aa4\u3002\u672c\u6587\u5c06\u901a\u8fc7\u5b9e\u9645\u793a\u4f8b\uff0c\u4ecb\u7ecd\u548c\u6bd4\u8f83\u4e24\u79cd\u5e38\u89c1\u7684\u5f52\u4e00\u5316\u65b9\u6cd5\uff1a\u7ebf\u6027\u5f52\u4e00\u5316\uff08\u4e5f\u79f0\u4e3a RPM \u5f52\u4e00\u5316\uff09\u548c\u5c3a\u5bf8\u56e0\u5b50\u5f52\u4e00\u5316\uff08Size Factor Normalization\uff09\uff0c\u5e76\u89e3\u91ca\u5b83\u4eec\u5404\u81ea\u7684\u4f18\u7f3a\u70b9\u53ca\u5e94\u7528\u573a\u666f\u3002<\/p>\n\n\n\n

\u7ebf\u6027\u5f52\u4e00\u5316\uff08RPM \u5f52\u4e00\u5316\uff09<\/h2>\n\n\n\n

\u4ec0\u4e48\u662f\u7ebf\u6027\u5f52\u4e00\u5316\uff1f<\/h3>\n\n\n\n

\u7ebf\u6027\u5f52\u4e00\u5316\uff0c\u4e5f\u5e38\u88ab\u79f0\u4e3a\u6bcf\u767e\u4e07\u8bfb\u53d6\u6570\uff08RPM\uff0cReads Per Million\uff09\u5f52\u4e00\u5316\uff0c\u662f\u4e00\u79cd\u5c06\u6570\u636e\u6309\u6bd4\u4f8b\u7f29\u653e\u7684\u65b9\u6cd5\u3002\u5b83\u901a\u8fc7\u5c06\u6bcf\u4e2a\u6837\u672c\u7684\u8bfb\u53d6\u6570\u6807\u51c6\u5316\u4e3a\u6bcf\u767e\u4e07\u8bfb\u53d6\u6570\uff0c\u4ece\u800c\u4f7f\u5f97\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u7684\u8bfb\u53d6\u6570\u5177\u6709\u53ef\u6bd4\u6027\u3002\u8be5\u65b9\u6cd5\u7684\u6838\u5fc3\u601d\u60f3\u662f\u6839\u636e\u6837\u672c\u7684\u603b\u8bfb\u53d6\u6570\u8fdb\u884c\u8c03\u6574\uff0c\u786e\u4fdd\u5728\u6837\u672c\u95f4\u8fdb\u884c\u516c\u5e73\u6bd4\u8f83\u3002<\/p>\n\n\n\n

\u7ebf\u6027\u5f52\u4e00\u5316\u7684\u8be6\u7ec6\u8ba1\u7b97\u6b65\u9aa4<\/h3>\n\n\n\n

\u8ba9\u6211\u4eec\u901a\u8fc7\u4e00\u4e2a\u7b80\u5355\u7684\u4f8b\u5b50\u6765\u7406\u89e3\u7ebf\u6027\u5f52\u4e00\u5316\u7684\u8fc7\u7a0b\u3002\u5047\u8bbe\u6211\u4eec\u6709\u4e09\u4e2a\u6837\u672c\u548c\u56db\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u6570\u636e\uff1a<\/p>\n\n\n\n

\u57fa\u56e0<\/th>\u6837\u672c1<\/th>\u6837\u672c2<\/th>\u6837\u672c3<\/th><\/tr>
\u57fa\u56e0A<\/td>100<\/td>300<\/td>200<\/td><\/tr>
\u57fa\u56e0B<\/td>400<\/td>1200<\/td>800<\/td><\/tr>
\u57fa\u56e0C<\/td>800<\/td>2400<\/td>1600<\/td><\/tr>
\u57fa\u56e0D<\/td>50<\/td>150<\/td>100<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u6b65\u9aa41\uff1a\u8ba1\u7b97\u6bcf\u4e2a\u6837\u672c\u7684\u603b\u8bfb\u53d6\u6570<\/strong><\/p>\n\n\n\n

    \n
  • \u6837\u672c1<\/strong>\uff1a100 + 400 + 800 + 50 = 1350<\/strong><\/li>\n\n\n\n
  • \u6837\u672c2<\/strong>\uff1a300 + 1200 + 2400 + 150 = 4050<\/strong><\/li>\n\n\n\n
  • \u6837\u672c3<\/strong>\uff1a200 + 800 + 1600 + 100 = 2700<\/strong><\/li>\n<\/ul>\n\n\n\n

    \u6b65\u9aa42\uff1a\u8ba1\u7b97\u6bcf\u4e2a\u57fa\u56e0\u7684 RPM \u503c<\/strong><\/p>\n\n\n\n

    \u5bf9\u4e8e\u6bcf\u4e2a\u6837\u672c\u4e2d\u7684\u6bcf\u4e2a\u57fa\u56e0\uff0c\u4f7f\u7528\u4ee5\u4e0b\u516c\u5f0f\u8ba1\u7b97 RPM\uff1a<\/p>\n\n\n\n

      <\/span>   <\/span>\"\[ \text{RPM} = \frac{\text{基因的读取数}}{\text{样本的总读取数}} \times 1,000,000 \]\"<\/p><\/p>\n\n\n\n

    \u8ba1\u7b97\u7ed3\u679c\u5982\u4e0b\uff1a<\/p>\n\n\n\n

    \u57fa\u56e0<\/th>\u6837\u672c1\uff08RPM\uff09<\/th>\u6837\u672c2\uff08RPM\uff09<\/th>\u6837\u672c3\uff08RPM\uff09<\/th><\/tr>
    \u57fa\u56e0A<\/td>\"\frac{100}{1350} \times 1,000,000 \approx 74074.07\"<\/td>\"\frac{300}{4050} \times 1,000,000 \approx 74074.07\"<\/td>\"\frac{200}{2700} \times 1,000,000 \approx 74074.07\"<\/td><\/tr>
    \u57fa\u56e0B<\/td>\"\frac{400}{1350} \times 1,000,000 \approx 296296.30\"<\/td>\"\frac{1200}{4050} \times 1,000,000 \approx 296296.30\"<\/td>\"\frac{800}{2700} \times 1,000,000 \approx 296296.30\"<\/td><\/tr>
    \u57fa\u56e0C<\/td>\"\frac{800}{1350} \times 1,000,000 \approx 592592.59\"<\/td>\"\frac{2400}{4050} \times 1,000,000 \approx 592592.59\"<\/td>\"\frac{1600}{2700} \times 1,000,000 \approx 592592.59\"<\/td><\/tr>
    \u57fa\u56e0D<\/td>\"\frac{50}{1350} \times 1,000,000 \approx 37037.04\"<\/td>\"\frac{150}{4050} \times 1,000,000 \approx 37037.04\"<\/td>\"\frac{100}{2700} \times 1,000,000 \approx 37037.04\"<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

    \u4f18\u7f3a\u70b9\u5206\u6790<\/h3>\n\n\n\n

    \u4f18\u70b9\uff1a<\/strong><\/p>\n\n\n\n

      \n
    • \u7ebf\u6027\u5f52\u4e00\u5316\u65b9\u6cd5\u7b80\u5355\u6613\u61c2\uff0c\u8ba1\u7b97\u5feb\u6377\u3002<\/li>\n\n\n\n
    • \u80fd\u6709\u6548\u5904\u7406\u6d4b\u5e8f\u6df1\u5ea6\u4e0d\u540c\u7684\u6837\u672c\uff0c\u4f7f\u5404\u6837\u672c\u95f4\u6570\u636e\u5177\u6709\u53ef\u6bd4\u6027\u3002<\/li>\n<\/ul>\n\n\n\n

      \u7f3a\u70b9\uff1a<\/strong><\/p>\n\n\n\n

        \n
      • \u5bf9\u5f02\u5e38\u503c\u654f\u611f\u3002\u5982\u679c\u67d0\u4e2a\u57fa\u56e0\u5728\u67d0\u4e9b\u6837\u672c\u4e2d\u8868\u8fbe\u6781\u9ad8\uff0c\u5b83\u5c06\u663e\u8457\u5f71\u54cd\u5f52\u4e00\u5316\u7ed3\u679c\uff0c\u53ef\u80fd\u5bfc\u81f4\u5176\u4ed6\u57fa\u56e0\u7684\u8868\u8fbe\u6c34\u5e73\u88ab\u538b\u7f29\u3002<\/li>\n\n\n\n
      • \u5047\u8bbe\u6240\u6709\u57fa\u56e0\u7684\u8868\u8fbe\u53d8\u5316\u662f\u7ebf\u6027\u7684\uff0c\u672a\u8003\u8651\u5230\u57fa\u56e0\u8868\u8fbe\u7684\u590d\u6742\u6027\u3002<\/li>\n<\/ul>\n\n\n\n

        \u5c3a\u5bf8\u56e0\u5b50\u5f52\u4e00\u5316<\/h2>\n\n\n\n

        \u4ec0\u4e48\u662f\u5c3a\u5bf8\u56e0\u5b50\u5f52\u4e00\u5316\uff1f<\/h3>\n\n\n\n

        \u5c3a\u5bf8\u56e0\u5b50\u5f52\u4e00\u5316\u662f DESeq2<\/code> \u4e2d\u5e38\u7528\u7684\u4e00\u79cd\u5f52\u4e00\u5316\u65b9\u6cd5\uff0c\u4e13\u4e3a\u5904\u7406\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u800c\u8bbe\u8ba1\u3002\u5b83\u7684\u6838\u5fc3\u601d\u60f3\u662f\u901a\u8fc7\u8ba1\u7b97\u6bcf\u4e2a\u6837\u672c\u7684\u5c3a\u5bf8\u56e0\u5b50\uff08Size Factor\uff09\uff0c\u5e76\u4f7f\u7528\u8fd9\u4e9b\u56e0\u5b50\u5bf9\u57fa\u56e0\u8868\u8fbe\u6570\u636e\u8fdb\u884c\u5f52\u4e00\u5316\uff0c\u4ece\u800c\u6d88\u9664\u6837\u672c\u95f4\u7531\u4e8e\u6d4b\u5e8f\u6df1\u5ea6\u6216\u6280\u672f\u6027\u53d8\u5f02\u5bfc\u81f4\u7684\u5dee\u5f02\uff0c\u4f7f\u5f97\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u7684\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u66f4\u52a0\u53ef\u6bd4\u3002<\/p>\n\n\n\n

        \u5c3a\u5bf8\u56e0\u5b50\u5f52\u4e00\u5316\u7684\u8be6\u7ec6\u6b65\u9aa4<\/h3>\n\n\n\n

        \u8ba9\u6211\u4eec\u901a\u8fc7\u4e00\u4e2a\u66f4\u590d\u6742\u7684\u4f8b\u5b50\u6765\u7406\u89e3\u5c3a\u5bf8\u56e0\u5b50\u5f52\u4e00\u5316\u7684\u8fc7\u7a0b\uff1a<\/p>\n\n\n\n

        \u57fa\u56e0<\/th>\u6837\u672c1<\/th>\u6837\u672c2<\/th>\u6837\u672c3<\/th><\/tr>
        \u57fa\u56e0A<\/td>10<\/td>10000<\/td>20<\/td><\/tr>
        \u57fa\u56e0B<\/td>50<\/td>60<\/td>55<\/td><\/tr>
        \u57fa\u56e0C<\/td>200<\/td>180<\/td>190<\/td><\/tr>
        \u57fa\u56e0D<\/td>100<\/td>120<\/td>110<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

        \u7b2c\u4e00\u6b65\uff1a\u8ba1\u7b97\u6bcf\u4e2a\u57fa\u56e0\u7684\u51e0\u4f55\u5e73\u5747\u503c<\/h4>\n\n\n\n

        \u51e0\u4f55\u5e73\u5747\u503c\u7684\u8ba1\u7b97\u516c\u5f0f\u4e3a\uff1a<\/p>\n\n\n\n

          <\/span>   <\/span>\"\[ \text{几何平均值} = \left(\prod_{i=1}^{n} x_i\right)^{\frac{1}{n}} \]\"<\/p><\/p>\n\n\n\n

        \u5bf9\u4e8e\u6bcf\u4e2a\u57fa\u56e0\u7684\u51e0\u4f55\u5e73\u5747\u503c\u8ba1\u7b97\u5982\u4e0b\uff1a<\/p>\n\n\n\n

          \n
        • \u57fa\u56e0A<\/strong>: \"\sqrt[3]{10 \times 10000 \times 20} = \sqrt[3]{2000000} \approx 125.99\"<\/li>\n\n\n\n
        • \u57fa\u56e0B<\/strong>: \"\sqrt[3]{50 \times 60 \times 55} = \sqrt[3]{165000} \approx 54.86\"<\/li>\n\n\n\n
        • \u57fa\u56e0C<\/strong>: \"\sqrt[3]{200 \times 180 \times 190} = \sqrt[3]{6840000} \approx 190.57\"<\/li>\n\n\n\n
        • \u57fa\u56e0D<\/strong>: \"\sqrt[3]{100 \times 120 \times 110} = \sqrt[3]{1320000} \approx 109.63\"<\/li>\n<\/ul>\n\n\n\n

          \u7b2c\u4e8c\u6b65\uff1a\u8ba1\u7b97\u6bcf\u4e2a\u6837\u672c\u7684\u5c3a\u5bf8\u56e0\u5b50<\/h4>\n\n\n\n

          \u5bf9\u4e8e\u6bcf\u4e2a\u6837\u672c\uff0c\u8ba1\u7b97\u5176\u6240\u6709\u57fa\u56e0\u8868\u8fbe\u503c\u4e0e\u5bf9\u5e94\u51e0\u4f55\u5e73\u5747\u503c\u7684\u6bd4\u503c\u7684\u4e2d\u4f4d\u6570\uff0c\u5f97\u5230\u5c3a\u5bf8\u56e0\u5b50\uff1a<\/p>\n\n\n\n

            \n
          • \u6837\u672c1<\/strong>:\n
              \n
            • \u57fa\u56e0A: \"\frac{10}{125.99} \approx 0.079\"<\/li>\n\n\n\n
            • \u57fa\u56e0B: \"\frac{50}{54.86} \approx 0.912\"<\/li>\n\n\n\n
            • \u57fa\u56e0C: \"\frac{200}{190.57} \approx 1.049\"<\/li>\n\n\n\n
            • \u57fa\u56e0D: \"\frac{100}{109.63} \approx 0.912\"<\/li>\n\n\n\n
            • \u5c3a\u5bf8\u56e0\u5b50<\/strong>\uff1a0.912<\/strong>\uff08\u4e2d\u4f4d\u6570\uff09<\/li>\n<\/ul>\n<\/li>\n\n\n\n
            • \u6837\u672c2<\/strong>:\n
                \n
              • \u57fa\u56e0A: \"\frac{10000}{125.99} \approx 79.37\"<\/li>\n\n\n\n
              • \u57fa\u56e0B: \"\frac{60}{54.86} \approx 1.093\"<\/li>\n\n\n\n
              • \u57fa\u56e0C: \"\frac{180}{190.57} \approx 0.945\"<\/li>\n\n\n\n
              • \u57fa\u56e0D: \"\frac{120}{109.63} \approx 1.095\"<\/li>\n\n\n\n
              • \u5c3a\u5bf8\u56e0\u5b50<\/strong>\uff1a1.093<\/strong>\uff08\u4e2d\u4f4d\u6570\uff09<\/li>\n<\/ul>\n<\/li>\n\n\n\n
              • \u6837\u672c3<\/strong>:\n
                  \n
                • \u57fa\u56e0A: \"\frac{20}{125.99} \approx 0.159\"<\/li>\n\n\n\n
                • \u57fa\u56e0B: \"\frac{55}{54.86} \approx 1.002\"<\/li>\n\n\n\n
                • \u57fa\u56e0C: \"\frac{190}{190.57} \approx 0.997\"<\/li>\n\n\n\n
                • \u57fa\u56e0D: \"\frac{110}{109.63} \approx 1.003\"<\/li>\n\n\n\n
                • \u5c3a\u5bf8\u56e0\u5b50<\/strong>\uff1a1.002<\/strong>\uff08\u4e2d\u4f4d\u6570\uff09<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n\n

                  \u7b2c\u4e09\u6b65\uff1a\u5f52\u4e00\u5316\u57fa\u56e0\u8868\u8fbe\u6570\u636e<\/h4>\n\n\n\n

                  \u5c06\u6bcf\u4e2a\u57fa\u56e0\u7684\u8868\u8fbe\u91cf\u9664\u4ee5\u5bf9\u5e94\u6837\u672c\u7684\u5c3a\u5bf8\u56e0\u5b50\uff0c\u5f97\u5230\u5f52\u4e00\u5316\u540e\u7684\u57fa\u56e0\u8868\u8fbe\u6570\u636e\uff1a<\/p>\n\n\n\n

                  \u57fa\u56e0<\/th>\u6837\u672c1\uff08\u5c3a\u5bf8\u56e0\u5b50\u5f52\u4e00\u5316\uff09<\/th>\u6837\u672c2\uff08\u5c3a\u5bf8\u56e0\u5b50\u5f52\u4e00\u5316\uff09<\/th>\u6837\u672c3\uff08\u5c3a\u5bf8\u56e0\u5b50\u5f52\u4e00\u5316\uff09<\/th><\/tr>
                  \u57fa\u56e0A<\/td>\"\frac{10}{0.912} \approx 10.96\"<\/td>\"\frac{10000}{1.093} \approx 9147.12\"<\/td>\"\frac{20}{1.002} \approx 19.96\"<\/td><\/tr>
                  \u57fa\u56e0B<\/td>\"\frac{50}{0.912} \approx 54.82\"<\/td>\"\frac{60}{1.093} \approx 54.89\"<\/td>\"\frac{55}{1.002} \approx 54.89\"<\/td><\/tr>
                  \u57fa\u56e0C<\/td>\"\frac{200}{0.912} \approx 219.30\"<\/td>\"\frac{180}{1.093} \approx 164.68\"<\/td>\"\frac{190}{1.002} \approx 189.62\"<\/td><\/tr>
                  \u57fa\u56e0D<\/td>\"\frac{100}{0.912} \approx 109.65\"<\/td>\"\frac{120}{1.093} \approx 109.75\"<\/td>\"\frac{110}{1.002} \approx 109.78\"<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

                  \u7ed3\u679c\u7684\u6bd4\u8f83<\/h3>\n\n\n\n

                  \u5728\u8fd9\u4e2a\u6781\u7aef\u4f8b\u5b50\u4e2d\uff0c\u57fa\u56e0A\u5728\u6837\u672c2\u4e2d\u7684\u8868\u8fbe\u503c\u975e\u5e38\u9ad8\uff0c\u5bfc\u81f4\u5728 RPM \u5f52\u4e00\u5316\u4e2d\uff0c\u8fd9\u4e2a\u9ad8\u8868\u8fbe\u57fa\u56e0\u6781\u5927\u5730\u5f71\u54cd\u4e86\u5176\u4ed6\u57fa\u56e0\u7684\u76f8\u5bf9\u8868\u8fbe\u6c34\u5e73\u3002\u800c\u5728\u5c3a\u5bf8\u56e0\u5b50\u5f52\u4e00\u5316\u4e2d\uff0c\u901a\u8fc7\u8ba1\u7b97\u6bcf\u4e2a\u6837\u672c\u7684\u5c3a\u5bf8\u56e0\u5b50\uff0c\u5e76\u5c06\u57fa\u56e0\u8868\u8fbe\u91cf\u9664\u4ee5\u8fd9\u4e9b\u56e0\u5b50\uff0c\u6211\u4eec\u80fd\u591f\u66f4\u597d\u5730\u63a7\u5236\u6781\u7aef\u503c\u7684\u5f71\u54cd\uff0c\u4ece\u800c\u83b7\u5f97\u66f4\u4e3a\u5408\u7406\u7684\u5f52\u4e00\u5316\u7ed3\u679c\u3002<\/p>\n\n\n\n

                  \u4f18\u7f3a\u70b9\u5206\u6790<\/h3>\n\n\n\n

                  \u4f18\u70b9\uff1a<\/strong><\/p>\n\n\n\n

                    \n
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