{"id":6025,"date":"2024-09-16T20:57:00","date_gmt":"2024-09-16T12:57:00","guid":{"rendered":"https:\/\/fanyiming.life\/?p=6025"},"modified":"2025-12-21T01:04:25","modified_gmt":"2025-12-20T17:04:25","slug":"puromycin-%e5%af%b9-hek293t-%e7%bb%86%e8%83%9e%e5%bd%b1%e5%93%8d%e7%9a%84%e7%a0%94%e7%a9%b6","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=6025","title":{"rendered":"Puromycin \u6740HEK293T \u7ec6\u80de\u7ecf\u9a8c"},"content":{"rendered":"\n<h3 class=\"wp-block-heading\">\u6982\u8ff0<\/h3>\n\n\n\n<p>\u5728\u6700\u8fd1\u7684\u4e00\u9879\u5229\u7528 Puromycin \u7b5b\u9009\u5177\u6709\u6297\u6027\u7684HEK293T \u7ec6\u80de\u7684\u5b9e\u9a8c\u4e2d\uff0c\u6211\u89c2\u5bdf\u5230\u4e86\u5728\u4e0d\u540c\u836f\u7269\u6d53\u5ea6\u4ee5\u53ca\u4e0d\u540c\u7ec6\u80de\u5bc6\u5ea6\u6761\u4ef6\u4e0b\u7684\u4e00\u4e9b\u7279\u6b8a\u73b0\u8c61\u3002\u4ee5\u4e0b\u662f\u73b0\u8c61\u8bb0\u5f55\u4ee5\u53ca\u53ef\u80fd\u7684\u673a\u5236\u89e3\u91ca\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u4e3b\u8981\u53d1\u73b0<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6d53\u5ea6\u5dee\u5f02\u5bf9\u836f\u6548\u7684\u5f71\u54cd<\/strong>\uff1a\u5728\u4f7f\u7528 Puromycin \u7684\u6d53\u5ea6\u68af\u5ea6\u8fdb\u884c\u5b9e\u9a8c\u65f6\uff0c\u53d1\u73b01\u03bcg\/mL, 2\u03bcg\/mL, 3\u03bcg\/mL\u76f4\u523020\u03bcg\/mL Puromycin\u5747\u80fd\u6709\u6548\u6740\u4f24\u65e0\u6297\u6027\u57fa\u56e0\u7684HEK293T\u7ec6\u80de\uff0c\u4e14\u968f\u6740\u4f24\u901f\u5ea6\u4e0e\u836f\u7269\u6d53\u5ea6\u6b63\u76f8\u5173\u3002\u7136\u800c\uff0c\u66f4\u9ad8\u7684\u6d53\u5ea6\uff0850 \u03bcg\/mL \u548c 100 \u03bcg\/mL\uff09\u5bf9 HEK293T \u7ec6\u80de\u7684\u6740\u4f24\u6548\u679c\u53cd\u800c\u4e0d\u5982\u8f83\u4f4e\u7684\u6d53\u5ea6\uff08\u4f8b\u5982 1 \u03bcg\/mL \u548c 3 \u03bcg\/mL\uff09\uff0c\u9700\u8981\u66f4\u4e45\u7684\u65f6\u95f4\u6740\u6b7b\u5168\u90e8\u7ec6\u80de\u3002\u8fd9\u79cd\u6d53\u5ea6\u4e0e\u6548\u679c\u4e4b\u95f4\u7684\u975e\u7ebf\u6027\u5173\u7cfb\u867d\u7136\u4ee4\u4eba\u60ca\u8bb6\u3002<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"241\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/09\/20240916212611295-1024x241.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-6028\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/09\/20240916212611295-1024x241.png?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/09\/20240916212611295-300x71.png?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/09\/20240916212611295-768x181.png?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/09\/20240916212611295-1536x361.png?x-oss-process=style\/default 1536w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/09\/20240916212611295.png?x-oss-process=style\/default 1985w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>\u66f4\u4e00\u624b\u7684\u73b0\u8c61\u662f\uff1a50 \u03bcg\/mL \u548c 100 \u03bcg\/mL\u5904\u7406\u7684\u5934\u51e0\u5929\u7ec6\u80de\u4ecd\u7136\u662f\u8d34\u58c1\u7684\uff0c\u4e0d\u50cf\u4f4e\u6d53\u5ea6\u5df2\u7ecf\u51e0\u4e4e\u5168\u90e8\u5206\u6563\u72b6\u6f02\u6d6e\u3002<\/p>\n<\/blockquote>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u7ec6\u80de\u5bc6\u5ea6\u5bf9\u7ec6\u80de\u6b7b\u4ea1\u7684\u5f71\u54cd<\/strong>\uff1a\u7b2c\u4e00\u6b21\u5b9e\u9a8c\uff0c\u4f7f\u7528 1 \u03bcg\/mL \u7684 Puromycin \u8fde\u7eed\u5904\u7406 HEK293T \u7ec6\u80de 72 \u5c0f\u65f6\u5e76\u672a\u5f15\u8d77\u663e\u8457\u7684\u7ec6\u80de\u6b7b\u4ea1\u3002\u7136\u800c\uff0c\u7b2c\u4e8c\u6b21\u5b9e\u9a8c\uff0c\u7528\u76f8\u540c\u7684\u836f\u7269\u6d53\u5ea6\u548c\u5904\u7406\u65f6\u95f4\uff0c\u5374\u89c2\u5bdf\u5230\u4e86\u9884\u671f\u7684\u7ec6\u80de\u6b7b\u4ea1\u73b0\u8c61\uff0824h\u65f6\u5373\u53ef\u89c2\u5bdf\u5230\u6709\u65e0\u6297\u6027\u57fa\u56e0\u7684\u7ec6\u80de\u4e4b\u95f4\u7684\u660e\u663e\u5dee\u5f02\uff09\u3002\u4e24\u6b21\u5b9e\u9a8c\u7684\u552f\u4e00\u533a\u522b\u662f\u7ec6\u80de\u5bc6\u5ea6\uff0c\u7b2c\u4e00\u6b21\u5df2\u7ecf\u8fbe\u5230\u5927\u4e8e100% Confluency\uff0c\u800c\u7b2c\u4e8c\u6b21\u4e3a70%~80%\u5de6\u53f3\u3002\u8fd9\u8868\u660e\u7ec6\u80de\u5bc6\u5ea6\u53ef\u80fd\u5bf9 Puromycin \u7684\u654f\u611f\u6027\u6709\u663e\u8457\u5f71\u54cd\u3002<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">\u53ef\u80fd\u673a\u5236\u89e3\u91ca<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u9ad8\u6d53\u5ea6\u6096\u8bba<\/strong>\uff1a\u5728 Puromycin \u9ad8\u6d53\u5ea6\u73af\u5883\u4e0b\uff0c\u7ec6\u80de\u53ef\u80fd\u4f1a\u542f\u52a8\u4e00\u7cfb\u5217\u5e94\u6fc0\u53cd\u5e94\u673a\u5236\uff0c\u6bd4\u5982\u81ea\u566c\uff0c\u4ee5\u5e2e\u52a9\u5b83\u4eec\u5728\u77ed\u671f\u5185\u7ee7\u7eed\u5b58\u6d3b\u3002\u8fd9\u79cd\u73b0\u8c61\u53ef\u80fd\u89e3\u91ca\u4e86\u4e3a\u4ec0\u4e48\u5728\u9ad8\u6d53\u5ea6\u4e0b\uff0cPuromycin \u7684\u836f\u6548\u53cd\u800c\u964d\u4f4e\u4e86\u3002<\/li>\n\n\n\n<li><strong>\u7ec6\u80de\u5bc6\u5ea6\u4e0e\u836f\u7269\u8010\u53d7\u6027<\/strong>\uff1a\u5728\u7ec6\u80de\u5bc6\u5ea6\u8f83\u9ad8\u7684\u57f9\u517b\u6761\u4ef6\u4e0b\uff0c\u7ec6\u80de\u5bf9 Puromycin \u7684\u8010\u836f\u6027\u53ef\u80fd\u66f4\u5f3a\u3002\u8fd9\u662f\u56e0\u4e3a\u9ad8\u5bc6\u5ea6\u4f1a\u6539\u53d8\u7ec6\u80de\u5fae\u73af\u5883\uff0c\u5305\u62ec\u8425\u517b\u7269\u8d28\u7684\u4f9b\u5e94\u51cf\u5c11\u4ee5\u53ca\u4ee3\u8c22\u5e9f\u7269\u7684\u79ef\u7d2f\u589e\u52a0\uff0c\u8fd9\u4e9b\u90fd\u53ef\u80fd\u5f71\u54cd\u5230\u836f\u7269\u7684\u5438\u6536\u548c\u5206\u5e03\u3002\u53e6\u5916\uff0c\u9ad8\u5bc6\u5ea6\u6761\u4ef6\u4e0b\u7684\u7ec6\u80de\u8fd8\u53ef\u80fd\u901a\u8fc7\u6fc0\u6d3b\u67d0\u4e9b\u4fe1\u53f7\u901a\u8def\u6765\u589e\u5f3a\u751f\u5b58\u673a\u5236\uff0c\u4ece\u800c\u5bf9\u6297\u836f\u7269\u7684\u6bd2\u6027\u3002<\/li>\n\n\n\n<li><strong>\u836f\u7269\u5438\u6536\u7684\u9971\u548c\u6548\u5e94<\/strong>\uff1a\u7ec6\u80de\u5bf9 Puromycin \u7684\u5438\u6536\u53ef\u80fd\u5b58\u5728\u4e00\u4e2a\u4e0a\u9650\u3002\u4e00\u65e6\u8fbe\u5230\u4e86\u8fd9\u4e2a\u9971\u548c\u70b9\uff0c\u5373\u4f7f\u518d\u589e\u52a0\u836f\u7269\u6d53\u5ea6\u4e5f\u4e0d\u4f1a\u663e\u8457\u63d0\u9ad8\u7ec6\u80de\u6bd2\u6027\uff0c\u53cd\u800c\u53ef\u80fd\u5bfc\u81f4\u7ec6\u80de\u4ea7\u751f\u6297\u6027\u3002<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">\u6536\u83b7\u7684\u7ecf\u9a8c<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u4f18\u5316\u836f\u7269\u6d53\u5ea6<\/strong>\uff1a\u4e3a\u4e86\u6bcf\u4e00\u4e2a\u5177\u4f53\u7684\u5b9e\u9a8c\uff0c\u786e\u5b9a\u6700\u9002\u5408\u7684 Puromycin \u6d53\u5ea6\u662f\u81f3\u5173\u91cd\u8981\u7684\u3002\u901a\u8fc7\u8fdb\u884c\u7ec6\u80de\u6bd2\u6027\u5b9e\u9a8c\u548c\u7ed8\u5236\u5242\u91cf\u53cd\u5e94\u66f2\u7ebf\u53ef\u4ee5\u5e2e\u52a9\u907f\u514d\u7531\u4e8e\u9ad8\u6d53\u5ea6\u836f\u7269\u5f15\u8d77\u7684\u4e0d\u826f\u6548\u679c\u3002\uff08\u5f53\u7136\uff0c\u8fd9\u6b21\u662f\u7279\u6b8a\u60c5\u51b5\u624d\u7528\u5230\u9ad8\u6d53\u5ea6\uff0c\u6b63\u5e38\u4eba\u4e0d\u4f1a\u53bb\u752850\u03bcg\/mL\u8fd9\u4e48\u9ad8\u7684\u6d53\u5ea6\u53bb\u7b5b\u9009\uff09<\/li>\n<\/ul>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>\u76ee\u524d\u5b9e\u9a8c\u5ba4\u4e09\u79cd\u5de5\u5177\u7ec6\u80deNCCIT, HEK293T, K562\u5747\u5df2\u9a8c\u8bc1\u8fc7\uff0c\u4f7f\u75281\u03bcg\/mL Puromycin\u662f\u5408\u9002\u7684\u3002<\/p>\n<\/blockquote>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u8003\u8651\u7ec6\u80de\u5bc6\u5ea6<\/strong>\uff1a\u5728\u9ad8\u5bc6\u5ea6\u6761\u4ef6\u4e0b\u57f9\u517b\u7ec6\u80de\u53ef\u80fd\u4f1a\u589e\u52a0\u5b83\u4eec\u5bf9 Puromycin \u7684\u8010\u836f\u6027\u3002\u56e0\u6b64\uff0c\u5728\u8bbe\u8ba1\u5b9e\u9a8c\u65f6\uff0c\u5e94\u5f53\u8003\u8651\u901a\u8fc7\u63a7\u5236\u7ec6\u80de\u5bc6\u5ea6\u6765\u589e\u5f3a Puromycin \u7684\u7b5b\u9009\u6548\u679c\u3002<\/li>\n<\/ul>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>\u8fd9\u4e00\u70b9\u5e94\u8be5\u662f\u672c\u6587\u6700\u60f3\u8bb0\u5f55\u7684\u4e00\u4e2a\u7ed3\u8bba\u4e86\u3002<\/p>\n<\/blockquote>\n\n\n\n<h4 class=\"wp-block-heading\">\u7ed3\u8bba<\/h4>\n\n\n\n<p>\u8fd9\u4e00\u7cfb\u5217\u5b9e\u9a8c\u63ed\u793a\u4e86\u4e00\u4e2a\u91cd\u8981\u7684\u6559\u8bad\uff0c\u5373\u9ad8\u6d53\u5ea6\u7684\u836f\u7269\u5e76\u4e0d\u603b\u662f\u80fd\u591f\u5e26\u6765\u66f4\u597d\u7684\u6548\u679c\uff0c\u800c\u7ec6\u80de\u57f9\u517b\u73af\u5883\u4e2d\u7684\u53d8\u91cf\u5982\u7ec6\u80de\u5bc6\u5ea6\u5728\u836f\u7269\u6548\u679c\u4e2d\u626e\u6f14\u7740\u91cd\u8981\u89d2\u8272\u3002\u8fd9\u4e9b\u6d1e\u5bdf\u4e3a\u672a\u6765\u66f4\u6709\u6548\u5730\u4f7f\u7528 Puromycin 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