{"id":6168,"date":"2024-07-01T04:48:00","date_gmt":"2024-06-30T20:48:00","guid":{"rendered":"https:\/\/fanyiming.life\/?p=6168"},"modified":"2026-04-29T21:10:24","modified_gmt":"2026-04-29T13:10:24","slug":"%e7%ba%a2%e9%a2%9c%e5%bc%b9%e6%8c%87%e8%80%81","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=6168","title":{"rendered":"\u7ea2\u989c\u5f39\u6307\u8001"},"content":{"rendered":"<div class=\"music-player-container\" id=\"music-player-1\"\n            data-audio=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/09\/insomnia.m4a\"\n            data-lrc=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/09\/insomnia.lrc\"\n            data-theme=\"light\"\n            data-autoplay=\"false\"\n            data-collapsed=\"false\"\n            data-maxheight=\"400px\"\n            data-fontsize=\"14px\"\n            data-hide-toggle=\"false\"\n            data-loop=\"true\">\n            <div class=\"custom-player\">\n  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absolute;\n    top: 10px;\n    left: 10px;\n    right: 10px;\n    bottom: 10px;\n    border: 2px dashed #d7d7d7;\n    pointer-events: none;\n}\n\n\/* \u5173\u95ed\u6309\u94ae\u6837\u5f0f *\/\n.modal-content button.close {\n    position: absolute;\n    top: 10px;\n    right: 10px;\n    background-color: transparent;\n    border: none;\n    font-size: 16px;\n    cursor: pointer;\n    color: #333;\n    padding: 5px 10px;\n    z-index: 1000;\n}\n\n\/* \u7981\u6b62\u5916\u90e8\u9875\u9762\u6eda\u52a8 *\/\nbody.no-scroll {\n    overflow: hidden;\n}\n\n\/* \u54cd\u5e94\u5f0f\u5e03\u5c40 *\/\n@media (min-width: 480px) {\n    .letter-paper {\n        width: calc(50% &#8211; 10px);\n    }\n}\n\n@media (min-width: 768px) {\n    .letter-paper {\n        width: calc(33.333% &#8211; 10px);\n    }\n\n    .letter-paper h4 {\n        font-size: 1.2em;\n    }\n}\n\n@media (min-width: 1024px) {\n    .letter-paper {\n        width: calc(25% &#8211; 10px);\n    }\n\n    .letter-paper h4 {\n        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class=\"wp-block-heading\">2024.3.1<\/h4>\n\n\n\n<p>\u6628\u665a\u867d\u7136\u65e9\u65e9\u8eba\u5728\u5e8a\u4e0a\uff0c\u4f46\u76f4\u5230\u51cc\u66682:00\u62163:00\u624d\u5165\u7761\u3002\u4eca\u5929\u9192\u6765\u5df2\u7ecf\u662f\u4e0b\u534813:30\u3002\u8d77\u5e8a\u540e\uff0c\u770b\u4e86\u4e24\u96c6\u66f4\u65b0\u7684\u89c6\u9891\u3002\u6d17\u6fa1\u540e\uff0c\u572815:00\u5de6\u53f3\u53bb\u4e86\u725b\u62c9\uff0c\u70b9\u4e86\u4efd\u5927\u76d8\u9e21\u996d\u3002<\/p>\n\n\n\n<p>\u56de\u5fc6\u8d77\u81ea\u5df1\u66fe\u8bbe\u5b9a\u8fc7\u768414\u5c0f\u65f6\u5de5\u4f5c\u6cd5\u2014\u2014\u4ece\u8fdb\u5165\u5b9e\u9a8c\u5ba4\u5f00\u59cb\u8ba1\u65f6\uff0c\u8fde\u7eed\u5de5\u4f5c14\u5c0f\u65f6\uff0c\u671f\u95f4\u53ea\u7b80\u5355\u7528\u9910\u3002\u51b3\u5b9a\u4eca\u5929\u518d\u6b21\u5c1d\u8bd5\u8fd9\u79cd\u65b9\u6cd5\u3002\u4e8e\u662f\u4ece15:30\u5f00\u59cb\u8ba1\u7b97\uff0c\u9884\u8ba1\u6301\u7eed\u5230\u660e\u65e95:30\u3002<\/p>\n\n\n\n<p>\u9996\u5148\uff0c\u68c0\u67e5\u4e86\u5b9e\u9a8c\u5ba4\u4e2d\u7684\u7ec6\u80de\uff1a24\u5b54\u677f\u91cc\u7684L1-MS2\u7ec6\u80de\u548c\u91ce\u751f\u578bNCCIT\u7ec6\u80de\u3002\u7ed3\u679c\u53d1\u73b0\uff0c\u4e24\u79cd\u7ec6\u80de\u90fd\u5df2\u7ecf\u53ef\u4ee5\u4f20\u4ee3\u3002L1-MS2\u7ec6\u80de\u5c06\u6309\u71671:3\u7684\u6bd4\u4f8b\u4f20\u4ee3\uff0c\u5e76\u51c6\u5907\u8f6c\u67d3RLIG1\u8d28\u7c92\uff1bNCCIT\u7ec6\u80de\u5219\u4f20\u4ee3\u5230\u76f8\u90bb\u5b54\u5185\u3002\u540c\u65f6\uff0c\u8fd8\u914d\u5236\u4e86\u4e00\u74f6\u65b0\u76841640\u57f9\u517b\u57fa\u3002<\/p>\n\n\n\n<p>\u63a5\u7740\uff0c\u8003\u8651\u5206\u6790\u4e4b\u524dASW-dTAG\u6784\u5efa\u5931\u8d25\u7684\u539f\u56e0\uff0c\u51b3\u5b9a\u4eceNCCIT\u91ce\u751f\u578b\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u5165\u624b\uff0c\u901a\u8fc7PCR\u548c\u4e00\u4ee3\u6d4b\u5e8f\u9a8c\u8bc1\u57fa\u56e0\u7ec4\u60c5\u51b5\u3002\u6839\u636e\u8ba1\u5212\uff0c\u9009\u62e9\u4e86\u76f8\u5e94\u7684\u5f15\u7269\u8fdb\u884cPCR\u53cd\u5e94\uff0c\u5e76\u914d\u5236\u4e86\u51dd\u80f6\u3002\u6b64\u65f6\uff0c\u65f6\u95f4\u6765\u5230\u4e8617:30\uff0c\u5de5\u4f5c\u4e86\u7ea62\u5c0f\u65f6\uff0c\u5269\u4f5912\u5c0f\u65f6\u3002<\/p>\n\n\n\n<p>\u67e5\u9605\u4e86\u4e4b\u524d\u6784\u5efa\u7684N-ASW-dTAG\u548cC-ASW-dTAG\u8d28\u7c92\u6d4b\u5e8f\u7ed3\u679c\uff0c\u53d1\u73b0\u7b26\u5408\u9884\u671f\uff0c\u8868\u660e\u540c\u6e90\u81c2\u5728\u57fa\u56e0\u7ec4\u4e2d\u5b58\u5728\u3002\u57fa\u4e8e\u6b64\uff0c\u8ba1\u5212\u9009\u62e9\u5408\u9002\u5f15\u7269\u8fdb\u884c\u540e\u7eed\u6d4b\u5e8f\u3002N\u7aef\u4ea7\u7269\u9009\u7528\u4e24\u7aef\u5f15\u7269\u53ca4226N-ASW-vR2\uff1bC\u7aef\u4ea7\u7269\u5219\u91c7\u7528\u4e24\u7aef\u5f15\u7269\u53caC-ASW-seqF2\u3002<\/p>\n\n\n\n<p>19:00\u65f6\uff0c\u5c06\u6d4b\u5e8f\u5e26\u653e\u5165\u4eea\u5668\uff0c\u5e76\u5728\u7b49\u5f85\u671f\u95f4\u6d4f\u89c8\u624b\u673a\u300220:00\u5f00\u59cb\u53c2\u52a0\u7ec4\u4f1a\uff0c\u4e3b\u8bb2\u4eba\u4e3aZT\u3002\u7ec4\u4f1a\u4e0a\u4e86\u89e3\u5230\u5218\u6d2a\u6c5f\u5373\u5c06\u9000\u5b66\uff0c\u8d74\u56fd\u5916\u653b\u8bfb\u535a\u58eb\u5b66\u4f4d\u7684\u6d88\u606f\uff0c\u5185\u5fc3\u9887\u611f\u590d\u6742\u3002<\/p>\n\n\n\n<p>\u7ec4\u4f1a\u7ed3\u675f\u540e\uff0c\u6574\u7406\u4e86\u9700\u8981\u67e5\u8be2\u7684\u51e0\u4e2a\u77e5\u8bc6\u70b9\uff1aH3K4me3, H3K9ac, H3K27ac\u7b49\u7ec4\u86cb\u767d\u4fee\u9970\u7684\u610f\u4e49\uff0csuper enhancer\u7684\u6982\u5ff5\u4ee5\u53caPol II 2P\u548c5P\u7684\u542b\u4e49\u3002<\/p>\n\n\n\n<p>21:45\u7ec4\u4f1a\u7ed3\u675f\u3002\u4e4b\u540e\u67e5\u9605\u8d44\u6599\uff0c\u6574\u7406\u4e86\u7ec4\u86cb\u767d\u7532\u57fa\u5316\u548c\u4e59\u9170\u5316\u4fee\u9970\u7684\u76f8\u5173\u4fe1\u606f\uff0c\u5e76\u786e\u8ba4\u4e86\u8fd9\u4e9b\u4fee\u9970\u53ef\u4ee5\u901a\u8fc7ChIP-seq\u6280\u672f\u6d4b\u5b9a\u3002\u5b9e\u9a8c\u5ba4\u4e2d\u4e5f\u627e\u5230\u4e86\u76f8\u5173\u7684\u6297\u4f53\u3002<\/p>\n\n\n\n<p>\u968f\u540e\uff0c\u5f00\u59cb\u7814\u7a76super enhancer\u7684\u76f8\u5173\u6982\u5ff5\uff0c\u5e76\u51b3\u5b9a\u9605\u8bfbRichard A. Young\u63d0\u51fa\u7684\u5173\u4e8esuper enhancer\u7684\u5f00\u521b\u6027\u8bba\u6587\uff0c\u5e76\u5236\u4f5c\u8bb2\u89e3\u89c6\u9891\u3002<\/p>\n\n\n\n<p>\u8bfb\u8bba\u6587\u7684\u8fc7\u7a0b\u4e2d\uff0c\u53d1\u73b0\u81ea\u5df1\u5bb9\u6613\u5206\u5fc3\uff0c\u591a\u6b21\u4e2d\u65ad\u53bb\u6d4f\u89c8\u793e\u4ea4\u5a92\u4f53\u3002\u6700\u7ec8\uff0c\u57285:42\u5b8c\u6210\u4e86\u8bba\u6587\u7684\u9605\u8bfb\uff0c\u51b3\u5b9a\u5728\u4e0b\u6b21\u5de5\u4f5c\u4e2d\u6574\u7406\u6210\u6587\u5b57\u548c\u89c6\u9891\u7b14\u8bb0\u3002<\/p>\n\n\n\n\n\n\n\n<p>\u4e0b\u73ed\uff01<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.3<\/h4>\n\n\n\n<p>\u4e0b\u534816:00\u9192\u6765\uff0c\u51c6\u5907\u8d77\u5e8a\u5403\u996d\u3002\u4eca\u5929\u7684\u4e3b\u8981\u4efb\u52a1\u5305\u62ec\u5904\u7406\u6bdb\u5e08\u5144\u7684\u5b9e\u9a8c\u8bb0\u5f55\u3001\u51c6\u5907\u5973\u751f\u8282\u6d3b\u52a8\u3001\u8fdb\u884c\u7ec6\u80de\u8f6c\u67d3\u7b49\u3002\u6d17\u6fa1\u3001\u5403\u996d\u540e\uff0c\u524d\u5f80\u7efc\u5408\u4f53\u80b2\u4e2d\u5fc3\u7ed9\u9f99\u65b0\u3001\u51a0\u52cb\u548c\u5f20\u4e16\u952c\u9001\u5973\u751f\u8282\u660e\u4fe1\u7247\u300218:00\u62b5\u8fbe\u5b9e\u9a8c\u5ba4\uff0c\u5f00\u59cb\u7b2c\u4e8c\u4e2a\u5de5\u4f5c\u65e5\uff0c\u8ba1\u5212\u6b21\u65e5\u65e9\u4e0a8\u70b9\u4e0b\u73ed\u3002<\/p>\n\n\n\n<p>\u4eca\u5929\u9700\u8981\u5b8c\u6210\u7684\u4efb\u52a1\uff1a\u4f20\u4ee3L1-MS2\u7ec6\u80de\u5e76\u8f6c\u67d3RLIG1-APEX2\uff1b\u68c0\u67e5ASW-dTAG\u8d28\u7c92\u6784\u5efa\u5e76\u91cd\u65b0\u8bbe\u8ba1\u5b9e\u9a8c\u6d41\u7a0b\uff1b\u89c2\u770b\u6bdb\u5e08\u5144\u53d1\u7684\u57fa\u91d1\u8d44\u6599\uff1b\u63a5\u624b\u5f20\u6d9b\u7684ANKRD11-APEX2\u6784\u5efa\uff1b\u590d\u4e60Ecoli RTCB 5\u2019OH RNA seq\u5e76\u68c0\u67e5\u8bd5\u5242\uff1b\u64b0\u5199super enhancer\u7684\u5e16\u5b50\uff1b\u51c6\u5907\u5973\u751f\u8282\u6d3b\u52a8\u7b49\u3002<\/p>\n\n\n\n<p>18:46\u5f00\u59cb\u4f20\u4ee3\u7ec6\u80de\uff0c\u5e76\u51bb\u5b58WT NCCIT\u7ec6\u80de\u300219:34\u5b8c\u6210\u7ec6\u80de\u64cd\u4f5c\uff0c\u8bbe\u5b9a\u4e86\u65e9\u4e0a8\u70b9\u7684\u95f9\u949f\u63d0\u9192\u81ea\u5df1\u8f6c\u67d3\u7ec6\u80de\u3002\u67e5\u770b\u524d\u4e00\u5929\u7684\u6d4b\u5e8f\u7ed3\u679c\uff0c\u672a\u53d1\u73b0\u5927\u95ee\u9898\u3002<\/p>\n\n\n\n<p>20:00\uff0c\u603b\u7ed3\u4e86\u6bdb\u5e08\u5144\u7684\u7814\u7a76\u6210\u679c\uff0c\u53d1\u73b0MCP1\u57fa\u56e0\u5f71\u54cd2-\u82ef\u4e59\u9187\u7684\u4ea7\u91cf\uff0c\u5e76\u901a\u8fc7\u8c03\u63a7ARO8, ARO9, ARO10\u7684\u86cb\u767d\u6c34\u5e73\u5b9e\u73b0\u8fd9\u4e00\u4f5c\u7528\u3002\u603b\u7ed3\u5b8c\u6bd5\u540e\uff0c\u51b3\u5b9a\u5148\u590d\u4e60ASW-dTAG\u5b9e\u9a8c\u8bb0\u5f55\uff0c\u5e76\u89c2\u770b\u76f8\u5173\u6559\u5b66\u89c6\u9891\u3002<\/p>\n\n\n\n<p>22:47\uff0c\u770b\u5b8c\u6559\u5b66\u89c6\u9891\u540e\uff0c\u5f00\u59cb\u8bbe\u8ba1\u4fee\u590d\u8d28\u7c92\u7684\u5f15\u7269\uff0c\u5e76\u601d\u8003\u5728\u7ec6\u80de\u7cfb\u6784\u5efa\u8fc7\u7a0b\u4e2d\u8bbe\u7f6e\u68c0\u67e5\u70b9\u7684\u65b9\u6cd5\u300223:21\uff0c\u68b3\u7406\u5b8c\u6d41\u7a0b\u540e\uff0c\u51b3\u5b9a\u5728\u8f6c\u67d3\u540e48\u5c0f\u65f6\u5bcc\u96c6mCherry\u9633\u6027\u7ec6\u80de\uff0c\u5e76\u572810\u5929\u540e\u518d\u6b21\u5bcc\u96c6GFP\u9633\u6027\u7ec6\u80de\u3002<\/p>\n\n\n\n<p>23:40\uff0c\u4e0b\u697c\u53d6\u5916\u5356\uff0c\u5e76\u4f11\u606f\u7247\u523b\u30021:20\uff0c\u5403\u5b8c\u87ba\u86f3\u7c89\u540e\uff0c\u5f00\u59cb\u8bbe\u8ba1\u5f15\u7269\u5e76\u63d0\u4ea4\u8ba2\u5355\u30024:00\uff0c\u5b8c\u6210\u5f15\u7269\u8bbe\u8ba1\uff0c\u8017\u65f62.5\u5c0f\u65f6\u3002\u603b\u7ed3\u5f53\u5929\u5de5\u4f5c\uff0c\u51b3\u5b9a\u8c03\u6574\u5de5\u4f5c\u65f6\u95f4\u4e3a10\u5c0f\u65f6\uff0c\u800c\u975e14\u5c0f\u65f6\u3002<\/p>\n\n\n\n<p>5:11\uff0c\u51b3\u5b9a\u4e0b\u73ed\uff0c\u5e76\u8ba1\u5212\u6b21\u65e5\u4e0b\u5348\u8fdb\u884c\u7ec6\u80de\u8f6c\u67d3\u3002<\/p>\n\n\n\n\n\n\n\n<p>\u770b\u5bf9\u65b9\u8fd9\u4e2a\u53cd\u9988\uff0c\u5e94\u8be5\u662f\u6ca1\u5565\u5e2e\u52a9&#8230;&#8230;<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"762\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/03\/20240303210125539-1024x762.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-1085\" style=\"width:393px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/03\/20240303210125539-1024x762.png?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/03\/20240303210125539-300x223.png?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/03\/20240303210125539-768x572.png?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/03\/20240303210125539.png?x-oss-process=style\/default 1166w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.4<\/h4>\n\n\n\n<p>\u7a81\u7136\u60f3\u8d77\u6765\u6211\u4e0d\u7528\u6d41\u5f0f\u554a\uff0c\u8d76\u7d27\u53d6\u6d88\u4e86\u3002\u6211\u662f\u836f\u6740\u7684\u3002<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.5<\/h4>\n\n\n\n<p>1:07\u5f00\u59cb\u65b0\u768410\u5c0f\u65f6\u5de5\u4f5c\u8ba1\u5212\uff0c\u76ee\u6807\u572811:07\u4e0b\u73ed\u3002\u4e3b\u8981\u4efb\u52a1\u5305\u62ec\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5b8c\u6210super enhancer\u6587\u7ae0\u7684\u6587\u5b57\u7248\u7b14\u8bb0\u548c\u89c6\u9891\u53d1\u5e03\u3002<\/li>\n\n\n\n<li>\u9605\u8bfb\u4e00\u7bc7LINE-1 RNA\u76f8\u5173\u6587\u7ae0\u5e76\u505a\u7b14\u8bb0\u3002<\/li>\n\n\n\n<li>\u590d\u4e60Ecoli RTCB 5&#8217;OH RNA seq\u7684protocol\u5e76\u68c0\u67e5\u836f\u54c1\u3002<\/li>\n\n\n\n<li>\u7b49\u5f85\u5f15\u7269\u5230\u8d27\u540e\u8fdb\u884cPCR\u3002<\/li>\n\n\n\n<li>\u63a5\u624b\u5f20\u6d9b\u7684ANKRD11 APEX2 knockin\u8d28\u7c92\u3002<\/li>\n<\/ol>\n\n\n\n<p>\u9996\u5148\u6574\u7406super enhancer\u7684\u6587\u5b57\u7b14\u8bb0\uff0c\u5b9e\u9645\u57283:18\u5b8c\u6210\u3002\u968f\u540e\u5236\u4f5cPPT\u5e76\u5f55\u5236\u89c6\u9891\uff0c5:03\u4e0a\u4f20\u81f3B\u7ad9\u548c\u5c0f\u7ea2\u4e66\u3002<\/p>\n\n\n\n<p>\u6b64\u65f6\u8ddd\u79bb\u4e0b\u73ed\u8fd8\u67096\u5c0f\u65f6\uff0c\u51b3\u5b9a\u4f11\u606f\u5e76\u5403\u6ce1\u9762\u30026:00\u7ed3\u675f\u4f11\u606f\uff0c\u51c6\u5907\u9605\u8bfbLINE-1 RNA\u76f8\u5173\u6587\u732e\uff0c\u4f46\u7531\u4e8e\u75b2\u60eb\uff0c\u51b3\u5b9a\u5148\u4f11\u606f\u3002<\/p>\n\n\n\n<p>7:45\u9192\u6765\uff0c\u4f9d\u7136\u56f0\u5026\uff0c\u672a\u80fd\u6709\u6548\u9605\u8bfb\u6587\u732e\u3002\u51b3\u5b9a\u7b49\u5f85\u5f15\u7269\u5230\u8d27\u540e\u518d\u8fdb\u884cPCR\u3002<\/p>\n\n\n\n<p>11:00\u9192\u6765\uff0c\u5b9e\u9645\u53ea\u5b8c\u6210\u4e864\u5c0f\u65f6\u5de5\u4f5c\u3002\u8003\u8651\u6539\u4e3a5\u5c0f\u65f6\u5de5\u4f5c\u5236\u3002<\/p>\n\n\n\n<p>\u665a\u4e0a23:00\u5f00\u59cb\u65b0\u76845\u5c0f\u65f6\u5de5\u4f5c\u8ba1\u5212\uff0c\u76ee\u6807\u5728\u6b21\u65e54:00\u4e0b\u73ed\u3002\u9996\u5148\u8fdb\u884c\u8dd1\u80f6\uff0c\u53d1\u73b0\u80f6\u5df2\u5e72\u88c2\uff0c\u91cd\u65b0\u914d\u80f6\u540e\u66f4\u6362\u7ec6\u80de\u57f9\u517b\u6db2\u3002<\/p>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.6<\/h4>\n\n\n\n<p>\u4eca\u5929\u5de5\u4f5c\u7279\u522b\u8f9b\u82e6\uff0c\u65e9\u4e0a4:30\u5b8c\u6210\u4e86\u8d28\u7c92\u6784\u5efa\u7684\u6d82\u677f\u5de5\u4f5c\u3002\u56de\u5230\u5bbf\u820d\u540e\uff0c\u4ece7:00\u4e00\u76f4\u7761\u523010:00\u3002\u8d77\u5e8a\u540e\uff0c\u524d\u5f80\u5706\u660e\u56ed\u67e5\u770b\u8bad\u7ec3\u573a\u5730\u7684\u60c5\u51b5\uff0c\u6d3b\u52a8\u4e00\u76f4\u6301\u7eed\u5230\u4e0b\u534813:00\u3002\u671f\u95f4\uff0c\u6211\u8fd8\u62bd\u7a7a\u5199\u4e86\u4e00\u4e9b\u6d3b\u52a8\u7b56\u5212\u7684\u5185\u5bb9\u3002<\/p>\n\n\n\n<p>14:30\u5de6\u53f3\u53bb\u5403\u4e86\u5348\u996d\uff0c\u4e4b\u540e\u56de\u5230\u4e86\u5bdd\u5ba4\u3002\u4e0b\u534816:30\u611f\u5230\u6709\u4e9b\u75b2\u60eb\uff0c\u518d\u6b21\u4f11\u606f\u4e86\u4e00\u4f1a\u513f\uff0c\u76f4\u523020:00\u624d\u9192\u6765\u3002\u5728\u5e8a\u4e0a\u7a0d\u5fae\u8eba\u4e86\u4e00\u4f1a\u513f\u540e\uff0c\u4e8e21:00\u51fa\u95e8\u5403\u4e86\u665a\u996d\u3002\u665a\u4e0a22:00\u5230\u8fbe\u5b9e\u9a8c\u5ba4\uff0c\u5374\u53d1\u73b0\u6709\u4e00\u4e2a\u5e73\u677f\u6ca1\u6709\u957f\u83cc\uff0c\u5fc3\u91cc\u6709\u4e9b\u5931\u843d\u3002<\/p>\n\n\n\n<p>\u51b3\u5b9a\u4ece22:00\u5f00\u59cb\u4e00\u4e2a\u65b0\u76845\u5c0f\u65f6\u5de5\u4f5c\u8ba1\u5212\uff0c\u76f4\u5230\u6b21\u65e5\u51cc\u66683:00\u3002\u9996\u5148\u6311\u9009\u4e86\u514b\u9686\uff0c\u5e76\u68c0\u67e5\u4e86\u7ec6\u80de\u662f\u5426\u9700\u8981\u66f4\u6362\u57f9\u517b\u6db2\u3002\u63a5\u7740\uff0c\u6211\u5f00\u59cb\u67e5\u627eC-ASW-dTAG\u6784\u5efa\u4e2d\u53ef\u80fd\u5b58\u5728\u7684\u95ee\u9898\u3002\u5982\u679c\u6709\u5fc5\u8981\u7684\u8bdd\uff0c\u8fd8\u9700\u8981\u8bbe\u8ba1\u65b0\u7684\u5f15\u7269\u5e76\u5bf9N-ASW-dTAG\u8fdb\u884cPCR\u9274\u5b9a\u3002<\/p>\n\n\n\n<p>22:20\u5f00\u59cb\u6447\u83cc\uff0c22:52\u5f00\u59cb\u590d\u82cfWT\u548cL1_5UTR-MS2(MCP-APEX2)\u7ec6\u80de\u3002<\/p>\n\n\n\n<p>\u5230\u4e86\u51cc\u66682:00\uff0c\u91cd\u65b0\u9176\u5207\u4e86C-ASW-dTAG\u7684\u7247\u6bb5\u548c\u8f7d\u4f53\uff0c\u5e76\u5bf9N-ASW-dTAG\u8fdb\u884c\u4e86PCR\u9274\u5b9a\u3002\u5f20\u6d9b\u63d0\u4f9b\u4e86ANKRD11-APEX2-KnockIn\u7684\u56fe\u8c31\uff0c\u6211\u6839\u636e\u56fe\u8c31\u5f00\u59cb\u8bbe\u8ba1\u5f15\u7269\u3002<\/p>\n\n\n\n<p>\u6ca1\u60f3\u5230\u8fd9\u6b21\u5de5\u4f5c\u8fdc\u8fdc\u8d85\u51fa\u4e86\u9884\u5b9a\u76845\u5c0f\u65f6\uff0c\u4e00\u76f4\u5fd9\u5230\u4e86\u51cc\u66685:30\uff0c\u5b9e\u9645\u5de5\u4f5c\u4e867.5\u5c0f\u65f6\u3002<\/p>\n\n\n\n<p>\u4e2d\u534812:00\uff0c\u67e5\u770b\u4e86L1(full length)-MS2\u7684\u6d41\u5f0f\u6570\u636e\uff0c\u9057\u61be\u7684\u662f\u7f3a\u5c11\u4e86WT\u5bf9\u7167\u7ec4\u7684\u6570\u636e\u3002<\/p>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.8<\/h4>\n\n\n\n<p>\u4eca\u5929\u4ece15:30\u5f00\u59cb\u5de5\u4f5c\uff0c\u8ba1\u5212\u572817:00\u7ed3\u675f\u3002\u9996\u5148\u8fdb\u884c\u4e86ANKRD11\u548cC-ASW-dTAG\u7684PCR\uff0c\u63a5\u7740\u68c0\u67e5\u4e86\u7ec6\u80de\u72b6\u6001\u3002\u6b64\u5916\uff0c\u8fd8\u9700\u5904\u7406\u6d41\u5f0f\u6570\u636e\u3002<\/p>\n\n\n\n<p>PCR\u8bbe\u7f6e\u4e86\u4e00\u4e2a\u5c0f\u65f6\u7684\u8fd0\u884c\u65f6\u95f4\uff0c\u6253\u7b97\u5728\u8fd9\u671f\u95f4\u5c0f\u61a9\u4e00\u4e0b\u3002\u539f\u672c\u8ba1\u52121\u70b9\u5f00\u59cb\u4f11\u606f\uff0c2\u70b9\u7ee7\u7eed\u5de5\u4f5c\uff0c\u7ed3\u679c\u4e00\u89c9\u9192\u6765\u5df2\u7ecf\u662f5\u70b9\u4e86\u3002<\/p>\n\n\n\n<p>\u4e0a\u5348\u4ece\u5403\u65e9\u996d\u5f00\u59cb\u5fd9\u788c\uff0c\u4e00\u76f4\u5e72\u5230\u4e5d\u70b9\u591a\uff0c\u4e4b\u540e\u5728\u697c\u9053\u7684\u6c99\u53d1\u4e0a\u4f11\u606f\uff0c\u76f4\u523011\u70b9\u624d\u9192\u6765\u3002\u4f11\u606f\u8fc7\u540e\uff0c\u53bb\u5212\u8239\u653e\u677e\u4e86\u4e00\u4e0b\u3002<\/p>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.9<\/h4>\n\n\n\n<p>\u665a\u4e0a22\u70b940\u5206\u5230\u8fbe\u5b9e\u9a8c\u5ba4\uff0c\u5f00\u59cb\u4e86\u65b0\u4e00\u8f6e\u7684\u5de5\u4f5c\u3002\u4eca\u665a\u7684\u4e3b\u8981\u4efb\u52a1\u5305\u62ec\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u6784\u5efa\u5b8cC-ASW-dTAG\u5e76\u5b8c\u6210\u6d82\u677f\u3002<\/li>\n\n\n\n<li>\u6784\u5efa\u5b8cANKRD11-APEX2\u5e76\u5b8c\u6210\u6d82\u677f\u3002<\/li>\n\n\n\n<li>\u6784\u5efa\u5b8cANKRD11-P2A-APEX2\u5e76\u5b8c\u6210\u6d82\u677f\u3002<\/li>\n\n\n\n<li>\u5c06APEX2-RLIG1\u548cRLIG1-APEX2\u4f20\u4ee3\u81f3\u4e0d\u52a0\u836f\u57f9\u517b\u57fa\uff0c\u5e76\u590d\u82cfAPEX2-V5\u5bf9\u7167\u3002<\/li>\n\n\n\n<li>\u901a\u8fc7Western blot\u9a8c\u8bc1\u6784\u5efa\u597d\u7684APEX2-RLIG1\u548cRLIG1-APEX2\u7ec6\u80de\u7cfb\u4e2d\u5b58\u5728V5-tag\u3002<\/li>\n\n\n\n<li>\u4f20\u4ee3L1(5UTR)-MS2(MCP-APEX2)\uff0c\u5e76\u51c6\u5907\u51bb\u5b58\u3002<\/li>\n\n\n\n<li>\u63d0\u53d6N-ASW-dTAG\u8d28\u7c92\uff0c\u5e76\u653e\u7f6e\u4e8e-80\u2103\u9187\u6c89\u3002<\/li>\n\n\n\n<li>\u5c06L1(full length)-MS2(MCP-APEX2)\u653e\u5165\u6db2\u6c2e\u7f50\u4fdd\u5b58\u3002<\/li>\n\n\n\n<li>\u5bf9L1(5UTR)-MS2(MCP-APEX2)\u548cL1(full length)-MS2(MCP-APEX2)\u8fdb\u884c\u6d41\u5f0f\u5206\u6790\uff0c\u5e76\u63d0\u524d\u67e5\u8be2\u4e0d\u540c\u8367\u5149\u901a\u9053\u5bf9\u5e94\u7684\u6ce2\u957f\u3002<\/li>\n<\/ol>\n\n\n\n<p>\u5de5\u4f5c\u5230\u6b21\u65e55:00\uff0c\u603b\u5171\u5de5\u4f5c\u4e866.33\u5c0f\u65f6\uff0c\u671f\u95f4\u53ea\u4f11\u606f\u4e8613\u5206\u949f\u3002\u9664\u4e86\u201c\u590d\u82cfAPEX2-V5\u5bf9\u7167\u201d\u4e4b\u5916\uff0c\u5176\u4f59\u4efb\u52a1\u5747\u5df2\u987a\u5229\u5b8c\u6210\u3002\u73b0\u5728\u611f\u5230\u6781\u5ea6\u56f0\u5026\uff0c\u51c6\u5907\u56de\u53bb\u7761\u89c9\u3002<\/p>\n\n\n\n<div class=\"globe\">\n\n    @import url(https:\/\/fonts.googleapis.com\/css?family=Fira+Sans:400,500italic);\n\n    .globe {\n        margin: 2vh 0 0 0; \/* \u4f7f\u7528\u89c6\u53e3\u9ad8\u5ea6\u5355\u4f4d *\/\n        position: relative;\n        display: block;\n        text-align: left;\n        width: 60vw; \/* \u57fa\u4e8e\u89c6\u53e3\u5bbd\u5ea6\u7684\u6bd4\u4f8b *\/\n        height: 60vw; \/* \u7ef4\u6301\u6b63\u65b9\u5f62\u6bd4\u4f8b *\/\n        margin: 0 auto; \/* \u5c45\u4e2d *\/\n        border-radius: 50%;\n        border: 3vw solid #f3f3f3; \/* \u8fb9\u6846\u4e5f\u91c7\u7528\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        box-sizing: border-box;\n        background: linear-gradient(to bottom, rgba(236, 240, 243, 1) 0%,rgb(228, 241, 249) 100%);\n        overflow: hidden;\n    }\n\n    .globe:before, .globe:after {\n        position: absolute;\n        content: &#8220;&#8221;;\n        box-sizing: border-box;\n        border-radius: 50%;\n        z-index: 10;\n    }\n\n    .globe:before {\n        height: 94%;\n        width: 94%;\n        top: 3%;\n        right: 3%;\n        border: 1.5vw solid transparent;\n        border-right-color: rgba(255,255,255,.3);\n        border-right-width: 1.5vw;\n        border-right-style: solid;\n    }\n\n    .globe:after {\n        top: 5vw;\n        right: 10vw;\n        width: 1.5vw;\n        height: 1.5vw;\n        background: rgba(255,255,255,.3);\n    }\n\n    .bird {\n        position: absolute;\n        z-index: 1;\n        left: 16vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: 17vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .body {\n        position: absolute;\n        width: 29.16vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 31vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50%;\n        background-clip: padding-box;\n        background-color: #11e7d7;\n        box-shadow: inset 0 0 80px rgba(0,0,0,.3);\n    }\n\n    .body:before, .body:after {\n        position: absolute;\n        content: &#8220;&#8221;;\n        z-index: -1;\n        width: 4.33vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 15.5vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50%;\n        background-color: #31bfae;\n        box-shadow: inset 0 0 13px rgba(0,0,0,.3);\n        top: 11.66vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        animation: wings var(&#8211;totaltime) linear infinite;\n    }\n\n    .body:before {\n        left: 0;\n    }\n\n    .body:after {\n        right: 0;\n    }\n\n    .eye {\n        position: absolute;\n        z-index: 1;\n        overflow: hidden;\n        width: 9.33vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 9.33vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: 4.66vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50%;\n        background-color: #fff;\n        border: 0.166vw solid #01c7be; \/* \u8fb9\u6846\u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        box-shadow: inset 0 0 0 0.166vw #01c7be; \/* \u76d2\u9634\u5f71\u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        animation: eyes var(&#8211;totaltime) linear infinite;\n    }\n\n    .eye:before {\n        position: absolute;\n        content: &#8220;&#8221;;\n        width: 3vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 3vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50%;\n        background-color: #000;\n    }\n\n    .eye:after {\n        position: absolute;\n        content: &#8220;&#8221;;\n        width: 33.33vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 33.33vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        background: radial-gradient(ellipse at center, rgba(109,0,25,0) 0%,rgba(130,1,31,0) 35%,#01c7be 36%,#31bfae 100%);\n        animation: eyes var(&#8211;totaltime) linear infinite;\n    }\n\n    .eye.left {\n        left: 2.5vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .eye.left:before {\n        left: 3.33vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .eye.left:after {\n        left: -10vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: -7.5vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .eye.right {\n        right: 2.5vw; \/* \u53f3\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .eye.right:before {\n        right: 3.33vw; \/* \u53f3\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .eye.right:after {\n        right: -10vw; \/* \u53f3\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: -7.5vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .beak {\n        position: absolute;\n        z-index: 1;\n        width: 6.83vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 9.16vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: 11.66vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        left: 11.16vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50%;\n        background-color: #31bfae;\n    }\n\n    .beak:before {\n        position: absolute;\n        content: &#8220;&#8221;;\n        width: inherit;\n        height: inherit;\n        top: 0.33vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50%;\n        background: #eb9f2d;\n    }\n\n    .beak:after {\n        position: absolute;\n        content: &#8220;&#8221;;\n        z-index: -1;\n        width: 6.16vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: inherit;\n        top: 2vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        left: 0.33vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50%;\n        background: #31bfae;\n    }\n\n    .beak &gt; div {\n        position: absolute;\n        width: 7.16vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 7.16vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: -0.166vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        left: -0.166vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50% 60% 50% 40%;\n        background-clip: padding-box;\n        background-color: #f7d35d;\n        transform: rotate(-45deg);\n    }\n\n    .beak &gt; div:before {\n        position: absolute;\n        content: &#8220;&#8221;;\n        transform: rotate(45deg);\n        width: 2.83vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 1.33vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: 1.66vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        left: 3.33vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50%;\n        background-color: #fff;\n    }\n\n    .feet {\n        position: absolute;\n        bottom: 2.5vw; \/* \u5e95\u90e8\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        width: 100%;\n    }\n\n    .feet:before, .feet:after {\n        position: absolute;\n        content: &#8220;&#8221;;\n        width: 4.5vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 3.5vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50%;\n        background-color: #f8c14d;\n        box-shadow: inset 0 0 12px rgba(0,0,0,.2);\n        animation: feet var(&#8211;totaltime) linear infinite;\n    }\n\n    .feet:before {\n        left: 6.66vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .feet:after {\n        right: 6.66vw; \/* \u53f3\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .wire {\n        position: absolute;\n        z-index: -1;\n        width: 83.33vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 66.66vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        left: -28.83vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: -35.83vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50%;\n        border: 0.5vw solid transparent; \/* \u8fb9\u6846\u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-bottom-color: black;\n    }\n\n    .hills {\n        position: absolute;\n        width: 10vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 10vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 30%;\n        top: 38.33vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        left: 10vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        transform: rotate(45deg);\n        background: radial-gradient(ellipse at top left, rgba(170,217,93,1) 0%,rgba(187,195,105,1) 100%);\n        box-shadow: inset 0.83vw 0 12px rgba(0,0,0,.2); \/* \u76d2\u9634\u5f71\u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .hills:before, .hills:after {\n        position: absolute;\n        content: &#8220;&#8221;;\n        width: 29.66vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 15vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 50%;\n        background: inherit;\n        box-shadow: inherit;\n    }\n\n    .hills:before {\n        left: -15vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: 5vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        z-index: -1;\n        transform: rotate(-20deg);\n    }\n\n    .hills:after {\n        left: 0;\n        top: -9.16vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        transform: rotate(120deg);\n    }\n\n    .cloud {\n        position: absolute;\n        width: 11.66vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 4vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        background: linear-gradient(to bottom, rgba(242,249,254,1) 5%,rgba(214,240,253,1) 100%);\n        border-radius: 3.33vw; \/* \u8fb9\u6846\u534a\u5f84\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: -3.33vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        z-index: -1;\n        animation: cloud calc(var(&#8211;totaltime) * 2) linear infinite;\n    }\n\n    .cloud:before, .cloud:after {\n        position: absolute;\n        content: &#8220;&#8221;;\n        background: inherit;\n        z-index: -1;\n    }\n\n    .cloud:before {\n        width: 6vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 6vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: -3vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        right: 1.66vw; \/* \u53f3\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 6.66vw; \/* \u8fb9\u6846\u534a\u5f84\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .cloud:after {\n        width: 3.33vw; \/* \u5bbd\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        height: 3.33vw; \/* \u9ad8\u5ea6\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        top: -1.66vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        left: 1.66vw; \/* \u5de6\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        border-radius: 3.33vw; \/* \u8fb9\u6846\u534a\u5f84\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n    }\n\n    .cloud.small {\n        top: -8.33vw; \/* \u4e0a\u8fb9\u8ddd\u8c03\u6574\u4e3a\u89c6\u53e3\u5bbd\u5ea6\u6bd4\u4f8b *\/\n        transform: scale(.6);\n        animation-delay: calc(-var(&#8211;totaltime) \/ 3);\n        animation-duration: calc(var(&#8211;totaltime) * 3);\n    }\n\n    \/\/ \u5b9a\u4e49\u5173\u952e\u5e27\u52a8\u753b\n    @keyframes eyes {\n        0%, 11.11%, 100% { top: -8.33vw; }\n        11.11% { top: -7.5vw; }\n        47.22% { top: -3.33vw; }\n        50%, 83.33% { top: -2.5vw; }\n        55.55% { top: -4.16vw; }\n    }\n\n    @keyframes wings {\n        0%, 83.33%, 100% { top: 11.66vw; }\n        55.55% { top: 10.0vw; }\n    }\n\n    @keyframes feet {\n        0%, 83.33%, 100% { top: -0.33vw; }\n        55.55% { top: 0; }\n    }\n\n    @keyframes cloud {\n        0% { left: -23.33vw; }\n        100% { left: 46.66vw; }\n    }\n\n    \/* \u6dfb\u52a0\u5a92\u4f53\u67e5\u8be2\u4ee5\u4f18\u5316\u5c0f\u5c4f\u5e55\u4e0a\u7684\u663e\u793a *\/\n    @media (max-width: 600px) {\n        .globe {\n            width: 80vw; \/* \u5728\u8f83\u5c0f\u5c4f\u5e55\u4e0a\u589e\u52a0\u5360\u6bd4 *\/\n            height: 80vw;\n            border: 4vw solid #f3f3f3; \/* \u8c03\u6574\u8fb9\u6846\u5bbd\u5ea6 *\/\n        }\n\n        .bird {\n            left: 13.33vw; \/* \u8c03\u6574\u5c0f\u9e1f\u4f4d\u7f6e *\/\n            top: 13.33vw;\n        }\n\n        .body {\n            width: 32vw; \/* \u8c03\u6574\u8eab\u4f53\u5927\u5c0f *\/\n            height: 32vw;\n        }\n\n        .body:before, .body:after {\n            width: 4.66vw; \/* \u8c03\u6574\u7fc5\u8180\u5927\u5c0f *\/\n            height: 16.66vw;\n            top: 10.66vw;\n        }\n\n        .eye {\n            width: 10vw; \/* \u8c03\u6574\u773c\u775b\u5927\u5c0f *\/\n            height: 10vw;\n            top: 5.33vw;\n        }\n\n        .eye:before {\n            width: 3.33vw; \/* \u8c03\u6574\u77b3\u5b54\u5927\u5c0f *\/\n            height: 3.33vw;\n        }\n\n        .eye:after {\n            width: 36.66vw; \/* \u8c03\u6574\u773c\u775b\u9634\u5f71\u5927\u5c0f *\/\n            height: 36.66vw;\n        }\n\n        .eye.left, .eye.right {\n            left: 3.33vw; \/* \u8c03\u6574\u773c\u775b\u4f4d\u7f6e *\/\n            right: 3.33vw;\n        }\n\n        .eye.left:before, .eye.right:before {\n            left: 4.16vw; \/* \u8c03\u6574\u77b3\u5b54\u4f4d\u7f6e *\/\n            right: 4.16vw;\n        }\n\n        .eye.left:after, .eye.right:after {\n            left: -11.66vw; \/* \u8c03\u6574\u773c\u775b\u9634\u5f71\u4f4d\u7f6e *\/\n            right: -11.66vw;\n            top: -8.33vw;\n        }\n\n        .beak {\n            width: 7.66vw; \/* \u8c03\u6574\u5599\u5927\u5c0f *\/\n            height: 10vw;\n            top: 10.66vw;\n            left: 12.33vw;\n        }\n\n        .beak:before {\n            top: 0.5vw; \/* \u8c03\u6574\u5599\u5185\u90e8\u4f4d\u7f6e *\/\n        }\n\n        .beak:after {\n            width: 6.66vw; \/* \u8c03\u6574\u5599\u5185\u90e8\u5927\u5c0f *\/\n            top: 2.33vw;\n            left: 0.5vw;\n        }\n\n        .beak &gt; div {\n            width: 7.66vw; \/* \u8c03\u6574\u5599\u5c16\u7aef\u5927\u5c0f *\/\n            height: 7.66vw;\n            top: -0.33vw;\n            left: -0.33vw;\n        }\n\n        .beak &gt; div:before {\n            width: 3vw; \/* \u8c03\u6574\u5599\u5c16\u7aef\u7ec6\u8282\u5927\u5c0f *\/\n            height: 1.66vw;\n            top: 1.83vw;\n            left: 3.5vw;\n        }\n\n        .feet {\n            bottom: 3.33vw; \/* \u8c03\u6574\u811a\u7684\u4f4d\u7f6e *\/\n        }\n\n        .feet:before, .feet:after {\n            width: 5vw; \/* \u8c03\u6574\u811a\u7684\u5927\u5c0f *\/\n            height: 4vw;\n        }\n\n        .feet:before {\n            left: 7.33vw; \/* \u8c03\u6574\u811a\u7684\u4f4d\u7f6e *\/\n        }\n\n        .feet:after {\n            right: 7.33vw; \/* \u8c03\u6574\u811a\u7684\u4f4d\u7f6e *\/\n        }\n\n        .wire {\n            width: 88.33vw; \/* \u8c03\u6574\u7ebf\u5708\u5927\u5c0f *\/\n            height: 71.66vw;\n            left: -30.66vw;\n            top: -38.33vw;\n            border: 0.66vw solid transparent; \/* \u8c03\u6574\u7ebf\u5708\u8fb9\u6846\u5927\u5c0f *\/\n        }\n\n        .hills {\n            width: 11.66vw; \/* \u8c03\u6574\u5c71\u4e18\u5927\u5c0f *\/\n            height: 11.66vw;\n            top: 41.66vw;\n            left: 11.66vw;\n            box-shadow: inset 1vw 0 12px rgba(0,0,0,.2); \/* \u8c03\u6574\u5c71\u4e18\u9634\u5f71 *\/\n        }\n\n        .hills:before, .hills:after {\n            width: 32.66vw; \/* \u8c03\u6574\u5c71\u4e18\u9634\u5f71\u5927\u5c0f *\/\n            height: 16.66vw;\n        }\n\n        .hills:before {\n            left: -16.66vw; \/* \u8c03\u6574\u5c71\u4e18\u9634\u5f71\u4f4d\u7f6e *\/\n            top: 6.66vw;\n        }\n\n        .hills:after {\n            top: -10.66vw; \/* \u8c03\u6574\u5c71\u4e18\u9634\u5f71\u4f4d\u7f6e *\/\n        }\n\n        .cloud {\n            width: 13.33vw; \/* \u8c03\u6574\u4e91\u6735\u5927\u5c0f *\/\n            height: 4.66vw;\n            border-radius: 4vw; \/* \u8c03\u6574\u4e91\u6735\u5706\u89d2 *\/\n            top: -4.66vw; \/* \u8c03\u6574\u4e91\u6735\u4f4d\u7f6e *\/\n        }\n\n        .cloud:before {\n            width: 6.66vw; \/* \u8c03\u6574\u4e91\u6735\u90e8\u5206\u5927\u5c0f *\/\n            height: 6.66vw;\n            top: -4vw;\n            right: 2.33vw;\n            border-radius: 8vw; \/* \u8c03\u6574\u4e91\u6735\u90e8\u5206\u5706\u89d2 *\/\n        }\n\n        .cloud:after {\n            width: 3.66vw; \/* \u8c03\u6574\u4e91\u6735\u90e8\u5206\u5927\u5c0f *\/\n            height: 3.66vw;\n            top: -2.33vw;\n            left: 2.33vw;\n            border-radius: 4.66vw; \/* \u8c03\u6574\u4e91\u6735\u90e8\u5206\u5706\u89d2 *\/\n        }\n\n        .cloud.small {\n            top: -10.66vw; \/* \u8c03\u6574\u5c0f\u4e91\u6735\u4f4d\u7f6e *\/\n        }\n    }\n\n    <!-- HTML \u7ed3\u6784 -->\n    <div class=\"bird\">\n        <div class=\"body\">\n            <div class=\"eye left\">\n                <div><\/div>\n                <div><\/div>\n            <\/div>\n            <div class=\"eye right\">\n                <div><\/div>\n                <div><\/div>\n            <\/div>\n            <div class=\"beak\"><div><\/div><\/div>\n            <div class=\"feet\"><\/div>\n        <\/div>\n        <div class=\"wire\"><\/div>\n        <div class=\"hills\"><\/div>\n        <div class=\"cloud\"><\/div>\n        <div class=\"cloud small\"><\/div>\n    <\/div>\n<\/div>\n\n\n    \/\/ JavaScript \u52a8\u6001\u8bbe\u7f6e\u52a8\u753b\u603b\u65f6\u957f\n    document.documentElement.style.setProperty(&#8216;&#8211;totaltime&#8217;, &#8216;3.6s&#8217;);\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.12<\/h4>\n\n\n\n<p>\u56de\u987e\u8fc7\u53bb\u51e0\u5929\u7684\u60c5\u51b5\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>3\u670810\u65e5\u5728TBT\u5212\u8239\uff0c\u6ca1\u6709\u8bb0\u5f55\u65e5\u8bb0\u3002<\/li>\n\n\n\n<li>3\u670811\u65e5\u7ec4\u7ec7\u4e86\u5706\u660e\u56ed\u7684\u6d3b\u52a8\uff0c\u4e5f\u6ca1\u6709\u8bb0\u5f55\u3002\u4f46\u5b9e\u9645\u4e0a\uff0c3\u670810\u65e5\u665a\u4e0a\u52303\u670811\u65e5\u51cc\u66684:00\u4e00\u76f4\u5728\u505a\u5b9e\u9a8c\u30023\u670811\u65e5\u665a\u4e0a\u56de\u5230\u5bb6\u540e\u592a\u7d2f\u4e86\uff0c\u76f4\u63a5\u7761\u5230\u4e863\u670812\u65e5\u4e0b\u5348\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u4eca\u5929\uff0c\u8001\u677f\u901a\u77e516:30\u53ec\u5f00\u5c0f\u7ec4\u4f1a\u8bae\u6c47\u62a5\u5de5\u4f5c\uff0c\u53ea\u5269\u4e0b1.5\u5c0f\u65f6\u7684\u65f6\u95f4\uff0c\u6211\u8ba1\u5212\u5b8c\u6210\u4e09\u4ef6\u4e8b\u60c5\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u53bb\u8377\u5858\u67e5\u770b\u6c34\u4f4d\u3002<\/li>\n\n\n\n<li>\u53bb\u897f\u4f53\u80b2\u9986\u786e\u8ba4\u665a\u4e0a\u662f\u5426\u53ef\u4ee5\u767b\u8bb0\u5e76\u8bad\u7ec3\u3002<\/li>\n\n\n\n<li>\u5b8c\u6210\u6628\u5929\u9f99\u62ac\u5934\u7684\u63a8\u9001\u3002<\/li>\n<\/ol>\n\n\n\n<p>16:20\u65f6\uff0c\u53ea\u5b8c\u6210\u4e86\u7b2c3\u4ef6\u4e8b\u60c5\u3002<\/p>\n\n\n\n<p>\u4e0b\u5348\u5f00\u5b8c\u5c0f\u7ec4\u4f1a\u8bae\u540e\uff0c\u524d\u5f80\u82cf\u4e16\u6c11\u4e66\u9662\u4e0e\u542f\u5143\u8ba8\u8bba\u4e86\u8bad\u7ec3\u5b89\u6392\uff0c\u5e76\u5728\u9014\u4e2d\u67e5\u770b\u4e86\u8377\u5858\u6c34\u4f4d\uff0c\u8ba4\u4e3a\u60c5\u51b5\u826f\u597d\u3002\u665a\u4e0a\u8bad\u7ec3\u7ed3\u675f\u540e\uff0c\u8be2\u95ee\u4e86\u897f\u4f53\u80b2\u9986\u7684\u53ef\u7528\u65f6\u95f4\uff0c\u5f97\u77e5\u5468\u4e00\u3001\u5468\u56db\u665a\u4e0a18:30\u523022:30\u53ef\u4ee5\u52a0\u7ec3\u3002<\/p>\n\n\n\n<p>\u4eca\u5929\u51e0\u4e4e\u5fd9\u5230\u4e86\u6df1\u591c\uff0c\u53ef\u4ee5\u8bf4\u53c8\u6ca1\u6709\u5b8c\u6210\u591a\u5c11\u5b9e\u9a8c\u5de5\u4f5c\u3002<\/p>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.13<\/h4>\n\n\n\n<p>0:00\u68c0\u67e5\u4e86\u7ec6\u80de\u72b6\u6001\uff0c\u53d1\u73b0\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>APEX2-RLIG1\u548cRLIG1-APEX2\u53ef\u4ee51\u4f203\uff0c\u51c6\u5907\u51bb\u5b58\u3002<\/li>\n\n\n\n<li>L1_5UTR\u53ef\u4ee5\u51bb\u5b58\uff0c\u75591\/3\u76846\u5b54\u677f\u7ec6\u80de\u7ee7\u7eed\u4f20\u4ee3\u3002<\/li>\n\n\n\n<li>L1_full\u4e5f\u53ef\u4ee5\u51bb\u5b58\uff0c\u75591\/3\u76846\u5b54\u677f\u7ec6\u80de\u7ee7\u7eed\u4f20\u4ee3\u3002<\/li>\n\n\n\n<li>SRP9\u7684dTAG\u5f00\u59cb\u52a0\u836f\uff0c\u8ba1\u521210:00\u6536\u83b7\u7ec6\u80de\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u65e9\u4e0a7:00\u8d77\u5e8a\uff0c8:00\u5bf9SRP14\u7684dTAG\u5f00\u59cb\u52a0\u836f\u3002<\/p>\n\n\n\n<p>\u4e0b\u53482:27\uff0c\u5b8c\u6210\u4e86\u6240\u6709\u7ec6\u80de\u7684\u64cd\u4f5c\u3002\u5f00\u59cb\u8bb0\u5f55\u5b9e\u9a8c\u5e76\u67e5\u770b\u6d4b\u5e8f\u7ed3\u679c\u3002\u5728\u5b9e\u9a8c\u5ba4\u4f11\u606f\u5230\u65e9\u4e0a7:50\uff0c\u7136\u540e\u5f00\u59cb\u7ed9SRP14\u52a0\u836f\u3002<\/p>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.14<\/h4>\n\n\n\n<p>15:50\uff0c\u5148\u628a\u6297\u4f53\u62ff\u51fa\u6765\u5316\u51bb\u3002\u7136\u540e\u6574\u7406\u4e00\u4e0b\u6700\u8fd1\u7684\u9f99\u821f\u961f\u7167\u7247\u3002\u4e0a\u4f20\u5230\u7f51\u7ad9\u3002<\/p>\n\n\n\n<p>\u665a\u4e0a23\u70b9\u6577\u4e0a\u4e86\u4e00\u6297\uff0canti-HA\u627eABclonal\u7684\u53d1\u73b0\u4e0d\u591f\u4e86\uff0c\u4e8e\u662f\u7528\u4e86CST\u7684C29F4.\u7136\u540e\u5c31\u4e0b\u73ed\u4e86\u3002\u56e0\u4e3a\u592a\u56f0\u3002<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.15<\/h4>\n\n\n\n<p>\u4eca\u592916:00\u5230\u8fbe\u5b9e\u9a8c\u5ba4\uff0c\u9996\u5148\u4ece4\u2103\u51b0\u7bb1\u4e2d\u53d6\u51faWestern Blot\u819c\uff0c\u5f00\u59cb\u6d17\u6da4\u4e00\u6297\u3002\u671f\u95f4\u914d\u5236\u4e862\u5347\u7684PBST\uff0c\u65f6\u95f4\u6765\u523016:30\u3002<\/p>\n\n\n\n<p>\u968f\u540e\u68c0\u67e5\u4e86\u7ec6\u80de\u72b6\u6001\uff0c\u7531\u4e8e\u5df2\u7ecf\u63a5\u8fd172\u5c0f\u65f6\u672a\u5904\u7406\uff0c\u539f\u8ba1\u5212\u51bb\u5b58\u7684APEX2-RLIG1\u548cRLIG1-APEX2\u9700\u8981\u518d\u4f20\u4ee3\u4e00\u6b21\u624d\u80fd\u51bb\u5b58\u3002<\/p>\n\n\n\n<p>\u4eca\u5929\u7684\u4efb\u52a1\u5305\u62ec\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u8ba2\u8d2dP2A\u8d28\u7c92\u7684\u65b0\u5f15\u7269\u3002<\/li>\n\n\n\n<li>\u4f20\u4ee3\u7ec6\u80de\u3002<\/li>\n\n\n\n<li>\u68c0\u67e5\u7528\u4e8eAPEX2 labeling\u7684\u8bd5\u5242\u3002<\/li>\n\n\n\n<li>\u6447\u83cc\uff0c\u51c6\u5907C-ASW-dTAG\u548c\u4e0d\u5e26P2A\u7684ANKRD11\u8d28\u7c92\u3002<\/li>\n\n\n\n<li>\u5f00\u59cb\u9605\u8bfb\u6587\u732e\uff0c\u4e0d\u9650\u6570\u91cf\uff0c\u91cd\u5728\u5f00\u59cb\u3002<\/li>\n\n\n\n<li>\u5b8c\u6210Western Blot\u5b9e\u9a8c\u5e76\u83b7\u53d6\u7ed3\u679c\u3002<\/li>\n<\/ol>\n\n\n\n<p>16:50\u65f6\uff0c\u5f00\u59cb\u63a5\u83cc\u3002<\/p>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.18<\/h4>\n\n\n\n<p>\u4eca\u592916:00\u5230\u8fbe\u5b9e\u9a8c\u5ba4\uff0c\u51b3\u5b9a\u5148\u5904\u7406\u4e4b\u524d\u6447\u7684\u83cc\uff0c\u6254\u6389\u65e7\u7684\u83cc\u6db2\uff0c\u8ba1\u5212\u91cd\u65b0\u63a5\u83cc\u3002<\/p>\n\n\n\n<p>\u5f53\u524d\u7684\u4e3b\u8981\u4efb\u52a1\u5305\u62ec\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5206\u6790L1-MS2\u7684FACS\u6570\u636e\u3002<\/li>\n\n\n\n<li>\u8fdb\u884cAPEX2-RLIG1\u7684V5 Western Blot\u3002<\/li>\n\n\n\n<li>\u5bf9L1-MS2\u8fdb\u884cAPEX2 labeling\u3002<\/li>\n\n\n\n<li>\u7ed9APEX2-RLIG1\u52a0dox\uff0c24\u5c0f\u65f6\u540e\u8fdb\u884cAPEX2 labeling\u3002<\/li>\n\n\n\n<li>\u7ed9SRP9\u548cSRP14\u52a0dTAG\u3002<\/li>\n<\/ol>\n\n\n\n<p>\u6628\u665a0:45\u6dfb\u52a0\u4e86dox\u548cdTAG\uff0c\u4eca\u5929\u65e9\u4e0a9:15\u5f00\u59cb\u4e0a\u73ed\u3002\u5148\u7ed9SRP14\u6dfb\u52a02\u5c0f\u65f6\u7684dTAG\uff0c\u8ba1\u521211:20\u6536\u96c6\u7ec6\u80de\u505aWestern Blot\u3002<\/p>\n\n\n\n<p>\u4f20\u4ee3\u4fdd\u79cd\u7684SRP9\u548cSRP14\u5355\u514b\u9686\u523012\u5b54\u677f\uff0c\u5e76\u6536\u96c6\u52a0\u8fc7dox\u7684\u7ec6\u80de\uff0c\u6682\u65f6\u51bb\u5b58\u4e8e-20\u2103\uff0c\u7a0d\u540e\u4e0eSRP\u7ec6\u80de\u4e00\u8d77\u88c2\u89e3\u63d0\u53d6\u86cb\u767d\u3002<\/p>\n\n\n\n<p>\u4e0b\u5348\u68c0\u67e5\u4e86EF1a\/fulllengthL1\/L1_5UTR-tagBFP-MS2\u7684\u84dd\u8272\u8367\u5149\u9633\u6027\u7387\uff0c\u7ed3\u679c\u5747\u8f83\u597d\u3002<\/p>\n\n\n\n<p>14:27\u6d4b\u91cf\u4e86\u86cb\u767d\u6d53\u5ea6\uff0c\u5e76\u8ba1\u7b97\u4e86\u6bcf\u79cd\u6837\u54c1\u6240\u9700\u7684\u4f53\u79ef\u3002\u8ba1\u5212\u8fdb\u884c\u56db\u5757\u51dd\u80f6\u7535\u6cf3\u3002<\/p>\n\n\n\n<p>\u4e0b\u5348\u8fd8\u8fdb\u884c\u4e86EF1a-tagBFP-MS2, L1_ORF1-tagBFP-ORF2-MS2\u548cL1_5UTR-tagBFP-MS2\u7684APEX2 labeling\uff0c\u6bcf\u4e2a\u6837\u54c12\u4e2a6\u5b54\u5b54\uff0c\u7ec6\u80depellet\u6db2\u6c2e\u901f\u51bb\u540e\u51bb\u5728-80\u2103\u3002<\/p>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.19<\/h4>\n\n\n\n<p>\u4eca\u5929\u662f\u9633\u95f4\u505a\u5b9e\u9a8c\u7684\u4e00\u5929\uff0c\u4e5d\u70b9\u591a\u5230\u5b9e\u9a8c\u5ba4\u5f00\u59cb\u5de5\u4f5c\u3002\u539f\u8ba1\u5212\u6628\u665a23\u70b9\u6765\u5b9e\u9a8c\u5ba4\uff0c\u4f46\u56e0\u56f0\u610f\u672a\u6267\u884c\u3002Western Blot\u819c\u5728\u5ba4\u6e29\u5c01\u95ed\u4e86\u5341\u51e0\u4e2a\u5c0f\u65f6\uff0c\u4eca\u5929\u67e5\u770b\u65f6\u53d1\u73b0\u4fe1\u53f7\u8f83\u5f31\u3002\u539f\u672c\u8ba1\u5212\u6628\u665a\u8fdb\u884cRLIG1\u7684APEX2 labeling\uff0c\u4f46\u7531\u4e8e\u7ec6\u80de\u751f\u957f\u8fc7\u6ee1\uff0c\u53ea\u80fd\u91cd\u65b0\u4f20\u4ee3\u94fa\u677f\u3002<\/p>\n\n\n\n<p>\u4e00\u6297\u53ef\u80fd\u5ba4\u6e291\u5c0f\u65f6\u7684\u6548\u679c\u4e0d\u59824\u2103\u8fc7\u591c\uff0c\u4e0b\u5348\u89c2\u5bdfWestern Blot\u7ed3\u679c\u65f6\u53d1\u73b0\u4fe1\u53f7\u8f83\u6d45\u3002anti-V5\u53ea\u68c0\u6d4b\u5230\u4e86APEX2-V5\uff0c\u672a\u68c0\u6d4b\u5230APEX2-V5-RLIG1\u548cRLIG1-V5-APEX2\uff0c\u53ef\u80fd\u662f\u56e0\u4e3a\u88ab\u5207\u5272\u6389\u4e86\u3002\u6240\u6709PVDF\u819c\u5df2\u653e\u7f6e\u57284\u2103\uff0c\u8ba1\u5212\u660e\u5929\u518d\u8fdb\u884c\u957f\u65f6\u95f4\u66dd\u5149\u3002<\/p>\n\n\n\n<p>\u5316\u51bb\u4e86\u6628\u5929\u51bb\u5b58\u7684MCP-MS2\u7ec6\u80de\uff0c\u4f7f\u7528RIPA buffer\u52a0cocktail\u548cPMSF\u88c2\u89e3\u7ec6\u80de\uff0c\u6bcf\u7ba1\uff081\u4e2a6\u5b54\u5b54\u7684\u7ec6\u80de\u91cf\uff09\u7528\u4e8650\u03bcl\u88c2\u89e3\u6db2\uff0c4\u2103\u65cb\u8f6c\u88c2\u89e330\u5206\u949f\u540e\uff0c13200rpm\u79bb\u5fc315\u5206\u949f\uff0c\u6536\u96c6\u4e8660\u03bcl\u7ec6\u80de\u88c2\u89e3\u6db2\u3002\u53d63\u03bcl\u7528\u4e8e\u6d4b\u91cf\u86cb\u767d\u6d53\u5ea6\uff0c\u4f46Bradford\u6cd5\u6d4b\u5f97\u7684\u7ed3\u679c\u4e0d\u51c6\u786e\u3002\u6700\u7ec8\u901a\u8fc7\u8089\u773c\u6bd4\u8272\u4f30\u8ba1\u4e86\u86cb\u767d\u6d53\u5ea6\uff0cEF1a\u7684rep1\u548crep2\u5747\u4e3a8\u03bcg\/\u03bcL\uff0c\u5176\u4ed6\u56db\u4e2a\u6837\u54c1\u5747\u4e3a10\u03bcg\/\u03bcL\u3002<\/p>\n\n\n\n<p>\u53d6\u4e86200\u03bcg\u86cb\u767d\u4e0e40\u03bcL MyOne Streptavidin beads\u7ed3\u5408\uff0c\u7528RIPA buffer\u8865\u5145\u81f3200\u03bcL\u4f53\u79ef\uff0c4\u2103\u65cb\u8f6c\u7ed3\u5408\uff0c\u4ece17\u70b9\u5f00\u59cb\u3002<\/p>\n\n\n\n<p>\u540c\u65f6\u63d0\u53d6\u4e86\u591a\u4e2a\u8d28\u7c92\uff0c\u5305\u62ecC-ASW-dTAG\u3001ANKRD11-N-APEX2-PGEMT\u3001sg1\u3001sg2\u3001sg3\u3002\u5f20\u6d9b\u5efa\u8bae\u4f7f\u7528PB-TetOn-APEX2-V5-BSD\u4f5c\u4e3a\u8d28\u8c31\u5bf9\u7167\u3002\u4ea4\u4ed8\u4e86ANKRD11-N-APEX2-PGEMT\uff0c\u81ea\u5df1\u7684C-ASW-dTAG\u548c\u53e6\u4e00\u4e2aN-ASW-dTAG\u653e\u7f6e\u5728-80\u2103\u9187\u6c89\uff0c\u8ba1\u5212\u660e\u5929\u4e00\u8d77\u6d17\u51fa\u7528\u4e8eASW-dTAG\u7684\u8f6c\u67d3\u3002<\/p>\n\n\n\n<p>\u4e0a\u5348\u8fd8\u4f20\u4ee3\u4e86NCCIT WT\u7ec6\u80de\uff0c\u5e76\u8bf7\u6559\u4e86\u5149\u8001\u5e08\u7684\u8f6c\u67d3\u7ecf\u9a8c\u3002\u6bcf\u4e2a6\u5b54\u5b54\u7528200\u03bcL opti-MEM\u57f9\u517b\u57fa\u7a00\u91ca3\u4e2a\u8d28\u7c92\u54041.5\u03bcg\uff0c\u5171\u8ba14.5\u03bcg\u8d28\u7c92\uff0c\u52a0\u516513.5\u03bcL Viafect\uff0c\u6df7\u540820\u5206\u949f\u540e\u653e\u5165\u4e00\u4e2a6\u5b54\u5b54\u3002<\/p>\n\n\n\n<p>14:35\u68c0\u67e5\u4e86\u7ec6\u80de\u5bc6\u5ea6\uff0c\u8ba4\u4e3aRLIG1\u7684dox\u5e94\u5728\u660e\u65e9\u6dfb\u52a0\u66f4\u4e3a\u5408\u9002\u3002WT\u7ec6\u80de\u4e5f\u6709\u4e9b\u7a00\u758f\uff0cASW-dTAG\u7684\u8f6c\u67d3\u540c\u6837\u9002\u5408\u5728\u660e\u65e9\u8fdb\u884c\u3002<\/p>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.20<\/h4>\n\n\n\n<p>\u4eca\u592913:15\u624d\u5230\u5b9e\u9a8c\u5ba4\uff0c\u6628\u665a\u8ba8\u8bba\u961f\u4f0d\u7ba1\u7406\u95ee\u9898\uff0c\u7761\u5f97\u5f88\u665a\uff0c\u5bfc\u81f4\u8d77\u5e8a\u665a\u4e86\uff0c\u803d\u8bef\u4e86\u4e0d\u5c11\u65f6\u95f4\u3002<\/p>\n\n\n\n<p>\u4eca\u5929\u7684\u4efb\u52a1\u5305\u62ec\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u6d17\u51fa\u9187\u6c89\u7684N-ASW-dTAG\u548cC-ASW-dTAG\u3002<\/li>\n\n\n\n<li>\u4e0b\u53483\u70b9\u53c2\u52a0\u5c0f\u7ec4\u4f1a\uff0c\u542c\u8bf4\u4ee5\u540e\u6bcf\u5468\u4e09\u90fd\u8981\u5f00\u6b63\u5f0f\u4f1a\u8bae\u3002<\/li>\n\n\n\n<li>\u51c6\u5907ASW-dTAG\u7684\u8f6c\u67d3\uff08\u7531\u4e8e\u7ec6\u80de\u592a\u7a00\u758f\uff0c\u63a8\u8fdf\u5230\u660e\u5929\uff09\u3002<\/li>\n\n\n\n<li>\u5904\u7406L1-MS2\u86cb\u767d\uff0c\u8fdb\u884cwashing\u548celuting\uff0c\u5e76\u51c6\u5907\u8dd1\u80f6\u3002<\/li>\n\n\n\n<li>\u9884\u7ea6\u5171\u4eab\u4eea\u5668\u5e73\u53f0\u7684EQ\uff08\u7528\u4e8e\u8f6c\u67d3\u540e48\u5c0f\u65f6\u7684ASW-dTAG\u9633\u6027\u5bcc\u96c6\uff09\u3002<\/li>\n\n\n\n<li>\u9884\u7ea6DIA-MS\uff0c\u8ba1\u5212\u660e\u5929\u4e0a\u5348\u9001\u6837\u3002<\/li>\n\n\n\n<li>\u663e\u5f71\u6628\u5929\u653e\u5728\u51b0\u7bb1\u91cc\u7684SRP9\/14\u5355\u514b\u9686\u7684WB\u7ed3\u679c\uff0c\u5e76\u6574\u7406\u7ed3\u679c\u56fe\u3002<\/li>\n\n\n\n<li>\u67e5\u660eAPEX2-V5-RLIG1\u672a\u66dd\u5149\u51fa\u6761\u5e26\u7684\u539f\u56e0\u3002<\/li>\n\n\n\n<li>\u7ed9\u4eca\u5929\u94fa\u57286\u5b54\u677f\u7684APEX2-V5, APEX2-V5-RLIG1\u548cRLIG1-V5-APEX2\u52a0dox\uff08\u63a8\u8fdf\u5230\u660e\u5929\uff09\u3002<\/li>\n<\/ol>\n\n\n\n<p>\u67e5\u4e86\u86cb\u767d\u8d28\u5927\u5c0f\uff1aAPEX2-V5 28.4kDa\uff0cAPEX2-V5-RLIG1 \u548c RLIG1-V5-APEX2 \u5747\u4e3a66.7kDa\u3002V5\u5e26\u786e\u5b9e\u572825\u548c35kDa\u4e4b\u95f4\uff0c\u4f46\u5176\u4ed6\u4e24\u4e2a\u6ca1\u6709\u5e26\u3002\u8ba1\u5212\u91cd\u65b0\u8dd1\u80f6\uff0c\u52a0\u5165-80\u2103\u51bb\u5b58\u7684AV\u548cAVR\u505a\u9633\u6027\u5bf9\u7167\u3002<\/p>\n\n\n\n<p>14:07\uff0c\u5f00\u59cb\u70ed\u57f9\u517b\u57fa\uff0c\u51c6\u5907\u52a0dox\uff0c\u5e76\u79bb\u5fc3N-ASW-dTAG\u548cC-ASW-dTAG\u8d28\u7c92\uff0c\u8ba1\u5212\u5728\u7ec4\u4f1a\u524d\u6d17\u51fa\u8d28\u7c92\u3002<\/p>\n\n\n\n<p>17:00\uff0c\u8ba1\u5212\u91cd\u65b0\u8fc7\u591c\u5b75\u80b2SRP14\u7684\u4e00\u6297\uff0c\u4e4b\u540e\u4e0eSRP9\u7684\u4e00\u8d77\u5b75\u80b2anti-ACTB\u3002\u51bb\u5b58SRP9N\u76849\u53f7\u548c16\u53f7\uff0cSRP14C\u768444\u53f7\u300154\u53f7\u548c55\u53f7\uff0c\u54042\u7ba1\u3002<\/p>\n\n\n\n<p>20:00\uff0c\u5f00\u59cb\u6d17\u6628\u592917:00\u5f00\u59cbbinding\u7684L1-MS2 streptavidin beads\uff0c\u6bcf\u6b21\u7528200\u03bcL buffer\u6d17\u3002\u6700\u540e\u73b0\u914dElution 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class=\"wp-block-heading\">2024.3.21<\/h4>\n\n\n\n<p>\u4eca\u5929\u4e0b\u534816:00\u624d\u5230\u5b9e\u9a8c\u5ba4\u3002\u9001\u4e86\u8d28\u8c316\u4e2a\u7ba1\u5b50\uff0c\u8ba2\u5355\u6807\u6ce8\u4e86DIA\u5b9a\u91cf\uff0c\u4f46\u53d1\u73b0\u9700\u8981\u63d0\u524d24\u5c0f\u65f6\u9884\u7ea6\u5e76\u901a\u8fc7\u5ba1\u6838\u624d\u80fd\u9001\u6837\u3002\u800c\u6211\u53ea\u662f\u63d0\u524d1\u5c0f\u65f6\u9884\u7ea6\uff0c\u5c1a\u672a\u901a\u8fc7\u5ba1\u6838\u3002\u6253\u7b97\u665a\u4e0a\u53bb\u770b\u770b\u6837\u54c1\u662f\u5426\u5df2\u88ab\u6536\u8d70\u3002<\/p>\n\n\n\n<p>\u5b8c\u6210\u4e86\u91cd\u65b0\u5b75\u80b2\u4e00\u6297\u7684SRP14\u9274\u5b9aWB\uff0c\u4f46\u65e0\u6cd5\u89e3\u91ca\u4e3a\u4ec0\u4e48anti-SRP14\u6ca1\u6709\u4efb\u4f55\u6761\u5e26\uff0c\u539f\u6765\u7684\u6761\u5e26\u4e5f\u4e0d\u89c1\u4e86\u3002anti-HA\u7684\u6761\u5e26\u6bd4\u4e4b\u524d\u5f3a\u4e00\u4e9b\uff0c\u53ef\u4ee5\u770b\u51faSRP14C 55#\u53f7\u5355\u514b\u9686\u57282\u5c0f\u65f65000\u500d\u7a00\u91ca\u7684dTAG\u5904\u7406\u4e0b\u4e0d\u80fd\u5b8c\u5168\u964d\u89e3\uff08\u6bd41\/4 0h\u8981\u5f3a\uff09\uff0c\u4f46\u8fd8\u6ca1\u6709\u5b75\u80b2actin\u3002\u8ba1\u5212\u8fc7\u591c\u5b75\u80b2\u03b2-actin\u3002<\/p>\n\n\n\n<p>\u4e0b\u534818:30\u8fdb\u884c\u4e86N-ASW-dTAG\u548cC-ASW-dTAG\u7684\u8f6c\u67d3\uff0c\u4f46\u8f6c\u5b8c\u540e\u53d1\u73b048\u5c0f\u65f6\u540e\u662f\u5468\u516d\uff0c\u65e0\u6cd5\u4f7f\u7528\u6d41\u5f0f\u5bcc\u96c6\u3002\u56e0\u6b64\u9884\u7ea6\u4e86\u660e\u5929\u5468\u4e9416:30\u7684\u6d41\u5f0f\u5bcc\u96c6\uff0c\u8fd9\u6837\u4e0d\u8db324\u5c0f\u65f6\uff0c\u6548\u7387\u53ef\u80fd\u4e0d\u9ad8\u3002\u540c\u65f6\u94fa\u4e863\u4e2aWT\u5b54\uff0c\u8ba1\u5212\u5468\u516d\u4e0a\u534811\u70b9\u91cd\u65b0\u8f6c\u67d3\uff0c\u5e76\u9884\u7ea6\u4e86\u5468\u4e0012:00~12:30\u7684\u65b0\u9986317 EQ\u5bcc\u96c6\uff0c\u6253\u7b97\u5c06\u8fd9\u4e24\u6b21\u5bcc\u96c6\u7684\u7ec6\u80de\u5408\u5e76\u3002<\/p>\n\n\n\n<p>\u4e0b\u534818:30\u5bf9APEX2-RLIG1\u7684\u4e09\u4e2a\u7ec6\u80de\u7cfb\u52a0\u4e86\u4e07\u5206\u4e4b\u4e00\u7684dox\uff0c\u6bcf\u4e2a\u7ec6\u80de\u7cfb\u4e24\u4e2a6\u5b54\u5b54\uff0c\u8ba1\u521224\u5c0f\u65f6\u540e\u8fdb\u884cAPEX2 labeling\u3002<\/p>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.22<\/h4>\n\n\n\n<p>\u4e0b\u534816:30\u53bbEQ\u5bcc\u96c6\u4e86ASW-dTAG\uff0c\u6536\u96c6\u4e86\u7ea625W\u7ec6\u80de\u3002\u672c\u6765\u6253\u7b9748\u5c0f\u65f6\u518d\u5bcc\u96c6\u6765\u7740\uff0c\u4f46\u662f\u6ca1\u7b97\u597d\u65f6\u95f4\uff0c\u53ea\u80fd\u5361\u5728\u5468\u4e94\u4e0b\u5348\u4e0d\u8db324h\u53bb\u5206\u3002\u4e0d\u8fc7\u8fd9\u6b21\u6536\u7684\u7ec6\u80de\u6570\u91cf\u8db3\u591f\u591a\uff08\u4e0a\u6b21\u5931\u8d25\u7684\u53ea\u67091.5W\uff09\u3002\u56de\u6765\u540e\u517b\u572824\u5b54\u677f\u3002<\/p>\n\n\n\n<p>\u665a\u4e0a\u663e\u5f71\u5b8c\u4e86SRP14\u7684\u4e24\u5f20actin\uff0c\u7136\u540e\u628aAPEX2-RLIG1\u548cRLIG1-APEX2\u7684\u6807\u8bb0\u7ed9\u505a\u4e86\u3002\u8fd8\u662f\u5e26\u4e0aAPEX2-V5\u505a\u5bf9\u7167\uff0cdox\u4e07\u5206\u4e4b\u4e00\u8bf1\u5bfc24h\uff0c\u6bcf\u4e2a\u7ec6\u80de\u7cfb2\u4e2a6-well\u5b54\uff0c\u8fc7\u6c27\u5316\u6c221mM\u5b75\u80b21min\u540e\u7ec8\u6b62\uff08\u4e0d\u8fc7\u6211\u5b9e\u9645\u64cd\u4f5c\u65f6\u6ce8\u610f\u5230\uff0c\u7b49\u5230\u52a0\u7ec8\u6b62\u5242\u5176\u5b9e\u5df2\u7ecf\u5feb1.5min\u4e86\uff09\uff0c\u7ec6\u80depellet\u6db2\u6c2e\u901f\u51bb\u540e\u5b58\u5728-80\u2103\u4e86\uff0c\u6ca1\u6709\u7ee7\u7eed\u5f80\u4e0b\u505a\uff0c\u56e0\u4e3a\u76ee\u524d\u8fd9\u4e24\u4e2a\u7ec6\u80de\u7cfb\u8fd8\u672a\u901a\u8fc7WB\u9274\u5b9a\uff08anti-V5\u7838\u4e0d\u5230\u5e26\uff09<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.25<\/h4>\n\n\n\n<p>\u53d1\u73b0C-ASW-dTAG\u6c61\u67d3\u4e86\uff0c\u91cd\u65b0\u8f6c\uff0c\u91cd\u65b0\u7ea6\u6d41\u5f0f\u3002WT\u91cd\u65b0\u94fa\u4e86\u677f\u3002<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.27<\/h4>\n\n\n\n<p>\u91cd\u65b0\u8f6c\u67d3\u4e86C-ASW-dTAG\uff0c\u8f6c\u67d3\u6761\u4ef6\u8ddf\u4e0a\u6b21\u5b8c\u5168\u76f8\u540c\u3002\u7528RLIG1\u6297\u4f53\u548cV5\u6297\u4f53\uff08\u90fd\u662frabbit\uff09\u8dd1\u4e86APEX2-V5, APEX2-V5-RLIG1, RLIG1-V5-APEX2\u7684WB\uff0c\u7528\u7684\u86cb\u767d\u8fd8\u662f\u4e0a\u6b21\u51bb\u5728-20\u7684\u3002APEX2-V5\u7684\u4e0d\u591f\u4e86\uff0c\u7406\u8bba\u4e0a30\u03bcg\u5e94\u8be5\u4e0a\u68374.9\u03bcL\uff0c\u4f46\u662f\u5b9e\u9645\u4e0a\u53ea\u4e0a\u6837\u4e863.5\u03bcL\u3002<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.3.29<\/h4>\n\n\n\n<p>\u4e2d\u534812:00\u8fdb\u884c\u4e8627\u53f7\u8f6c\u67d3\u7684C-ASW-dTAG\u7684\u5206\u9009\uff0c\u5bcc\u96c6\u4e86\u5927\u7ea680\u4e07\u4e2a\u7ec6\u80de\uff0c\u9633\u6027\u7387\u8d85\u8fc730%\u3002<\/p>\n\n\n\n<p>\u4e4b\u524d\u8ba4\u4e3a22\u53f7\u5bcc\u96c6\u56de\u6765\u7684C-ASW-dTAG\u53d7\u5230\u6c61\u67d3\uff0c\u4f46\u4eca\u5929\u68c0\u67e5\u540e\u53d1\u73b0\u5e76\u975e\u6c61\u67d3\u3002\u4e8e\u662f\u5c06\u7ec6\u80de\u4f20\u4ee3\u5230\u4e866\u5b54\u677f\u3002<\/p>\n\n\n\n<p>N-ASW-dTAG\u57286\u5b54\u677f\u4e2d\u957f\u6ee1\u4e86\uff0c\u56e0\u6b64\u5c06\u5176\u4f20\u4ee3\u5230\u4e86\u4e00\u4e2a10\u5398\u7c73\u7684\u57f9\u517b\u677f\u4e2d\u3002<\/p>\n\n\n\n<p>\u9884\u7ea6\u4e862024\u5e743\u670831\u65e5\uff08\u5468\u4e00\uff09\u4e0b\u534814\u70b9\u523015\u70b9\u7684EQ\uff0c\u8ba1\u5212\u5c0622\u53f7\u5bcc\u96c6\u7684N-ASW-dTAG\u548cC-ASW-dTAG\u6253\u677f\u5e76\u6536\u96c6population\u3002<\/p>\n\n\n\n<p>\u91cd\u65b0\u505a\u7684\u7528anti-V5 (rabbit)\u548canti-RLIG1 (rabbit)\u7684RLIG1\u7684Western Blot\u7ed3\u679c\u663e\u793a\uff0c\u4e24\u79cd\u6297\u4f53\u90fd\u80fd\u68c0\u6d4b\u5230\u6b63\u5e38\u5927\u5c0f\u7684\u6761\u5e26\u3002\u4e4b\u524d\u672a\u80fd\u68c0\u6d4b\u5230\u53ef\u80fd\u662f\u7531\u4e8e\u5b9e\u9a8c\u5931\u8bef\u672a\u5c06\u6837\u54c1\u6b63\u786e\u4e0a\u6837\u5230\u80f6\u5b54\u4e2d\u3002<\/p>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.4.1<\/h4>\n\n\n\n<p>\u4e0b\u534814:00\u5728\u663e\u5fae\u955c\u4e0b\u770b\u4e8622\u53f7\u5bcc\u96c6\u7684N-ASW-dTAG\uff08\u572810cm\u677f\uff09\u548cC-ASW-dTAG\uff08\u57286-well plate\uff09\uff0c\u90fd\u6ca1\u6709\u770b\u5230GFP\u4fe1\u53f7\uff0c\u4f46\u8fd8\u662f\u62b1\u7740\u4fa5\u5e78\u5fc3\u7406\u53bbFACS\u5bcc\u96c6\u4e86\u3002<\/p>\n\n\n\n\n\n\n\n<p>\u91cd\u65b0\u914d\u7f6e\u4e86RIPA buffer\u3002<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>\u6c34<\/td><td>38mL<\/td><\/tr><tr><td>1M Tris (pH7.5)<\/td><td>2.5mL<\/td><\/tr><tr><td>5M NaCl<\/td><td>1.5mL<\/td><\/tr><tr><td>10%SDS<\/td><td>0.5mL<\/td><\/tr><tr><td>10%\u8131\u6c27\u80c6\u9178\u94a0<\/td><td>2.5mL<\/td><\/tr><tr><td>10% Triton X-100<\/td><td>5mL<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\">50mL RIPA buffer<\/figcaption><\/figure>\n\n\n\n<p>\u4ece-80\u2103\u51b0\u7bb1\u53d6\u51fa22\u65e5\u6807\u8bb0\u7684APEX2-V5, APEX2-V5-RLIG1, RLIG1-V5-APEX2\u7ec6\u80de\uff08\u54042\u7ba1\uff09\uff0c\u52a0\u516555\u03bcL RIPA (+cocktail +PMSF)\uff0c4\u2103\u65cb\u8f6c30min\u88c2\u89e3\u7ec6\u80de\uff0c14000rpm\u79bb\u5fc315min\uff08\u8fd9\u91cc\u51fa\u73b0\u4e86\u5931\u8bef\uff0c\u79bb\u5fc3\u673a\u6e29\u5ea6\u8bbe\u7f6e\u9519\u4e86\uff0c\u5e94\u8be5\u662f4\u2103\uff0c\u5b9e\u9645\u662f25\u2103\uff09\u3002\u4fdd\u7559\u4e0a\u6e05\u3002<\/p>\n\n\n\n<p>\u6d4b\u86cb\u767d\u6d53\u5ea6\uff1a<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>V5 rep1<\/td><td>6.73ug\/ul<\/td><\/tr><tr><td>V5 rep2<\/td><td>21.04ug\/ul<\/td><\/tr><tr><td>APEX2-RLIG1 rep1<\/td><td>5.51ug\/ul<\/td><\/tr><tr><td>APEX2-RLIG1 rep2<\/td><td>5.85ug\/ul<\/td><\/tr><tr><td>RLIG1-APEX2 rep1<\/td><td>5.63ug\/ul<\/td><\/tr><tr><td>RLIG1-APEX2 rep2<\/td><td>7.66ug\/ul<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\">BradFord\u6d4b\u7684\uff0c\u4e24\u6b21\u6807\u51c6\u54c1\u7684A595\u4e0d\u4e00\u6837\uff0c \u7528\u4e86\u7b2c\u4e8c\u6b21\u7684\uff0c\u8d39\u89e3<\/figcaption><\/figure>\n\n\n\n<p>\u8ba1\u5212\u548c\u4e0a\u5468\u505aL1-MS2\u7684\u5b9e\u9a8c\u4e00\u6837\uff0c\u6bcf\u4e2atube\u91cc\u53d6\u4e24\u4efd200ug\u86cb\u767d\u51fa\u6765\uff0c\u5206\u522b\u4e0e40ul MyOne Streptavidin beads\u5b75\u80b2\u6765\u7740\uff0c\u4f46\u662f\u8ba1\u7b97\u4e86\u4e00\u4e0b\u8fd9\u6b21\u7684\u6d53\u5ea6\u4e0d\u591f\u6bcf\u4e2atube 400\u03bcg\u86cb\u767d\uff0c\u6240\u4ee5\u6bcf\u4e2a\u7ba1\u4e2d\u53d6\u51fa\u4e86300\u03bcg\u86cb\u767d\uff0c\u52a060\u03bcL beads\u5b75\u80b2\u3002\u6253\u7b97\u6253\u8d28\u8c31\u8fd8\u662f\u7528200\u03bcg\u86cb\u767d\uff0c\u4f46\u662f\u8dd1\u8003\u67d3\u548cStrep-WB\u7684\u4e0a\u6837\u51cf\u534a\u3002<\/p>\n\n\n\n<p>19\u70b9\u5f00\u59cb\u57284\u2103\u65cb\u8f6c\u5b75\u80b2\u3002<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.4.2<\/h4>\n\n\n\n<p>\u590d\u82cf\u523012\u5b54\u677f\uff1aSRP9C-5,9,22,28,34; SRP14C-37,42,44,54,55\uff0c\u90fd\u662fNCCIT\u4e2d\u7684dTAG\u5355\u514b\u9686\u3002<\/p>\n\n\n\n<p>\u6253\u7b97\u505aRIP\u548cRNA-seq\uff0cRIP\u53ef\u4ee55\u4e2a\u5355\u514b\u9686\u6df7\u8d77\u6765\u53d8\u6210\u4e00\u4e2a\u6837\u54c1\u505a\uff0cRNA-seq\u8981\u5355\u72ec\u505a\u3002<\/p>\n\n\n\n<p>\u5c06\u7b2c\u4e8c\u6279\u8f6c\u67d3\u7684C-ASW-dTAG\u7a7f\u4ee3\uff0c\u4ece6-well\u5b54\u52306-well\u5b54\uff0c\u94fa\u7684\u66f4\u52a0\u5206\u6563\u4e00\u70b9\u3002<\/p>\n\n\n\n<p><\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.4.3<\/h4>\n\n\n\n<p>3:00\u53d6\u51fa\u57284\u2103\u65cb\u8f6c\u7684APEX2-V5, APEX2-V5-RLIG1, RLIG1-V5-APEX2.\u51716\u4e2atube\uff0c\uff08\u4e00\u5171binding\u4e8632h\uff09\u3002\u6536\u96c6Flowthrough\u3002<\/p>\n\n\n\n<p>200\u03bcL RIPA buffer\u6d17\u4e24\u6b21.<\/p>\n\n\n\n<p>200\u03bcL 1M KCl\u6eb6\u6db2\u6d17\u4e00\u6b21.<\/p>\n\n\n\n<p>200\u03bcL 0.1M Na2CO3\u6eb6\u6db2\u6d17\u4e00\u6b21.<\/p>\n\n\n\n<p>200\u03bcL (2M\u5c3f\u7d20 in 10mM Tris-HCl, pH8.0)\u6d17\u4e00\u6b21.<\/p>\n\n\n\n<p>200\u03bcL RIPA Buffer\u6d17\u4e24\u6b21.<\/p>\n\n\n\n<p>30\u03bcL &nbsp;(3x protein loading buffer supplemented with 20mM DTT and 2mM biotin)\u6d17\u8131\uff0c95\u2103\u716e5min\u3002<\/p>\n\n\n\n<p>\u7528\u4e863\u57574~20% 15-well\u86cb\u767d\u80f6\u3002<\/p>\n\n\n\n<p>\u7b2c\u4e00\u5757\u505a\u8003\u67d3\uff1aMarker(plus), APEX2-V5 rep1(input 20\u03bcg, FL18.75\u03bcL, Elute 10\u03bcL), APEX2-V5-RLIG1 rep1(input 20\u03bcg, FL18.75\u03bcL, Elute 10\u03bcL), RLIG1-V5-APEX2 rep1(input 20\u03bcg, FL18.75\u03bcL, Elute 10\u03bcL), Marker<\/p>\n\n\n\n<p>\u7b2c\u4e8c\u5757\u505aStrep-WB: Marker(plus), APEX2-V5 rep2(input 20\u03bcg, FL18.75\u03bcL, Elute 10\u03bcL), APEX2-V5-RLIG1 rep2(input 20\u03bcg, FL18.75\u03bcL, Elute 10\u03bcL), RLIG1-V5-APEX2 rep2(input 20\u03bcg, FL18.75\u03bcL, Elute 10\u03bcL), Marker<\/p>\n\n\n\n<p>SE068 Strep-HRP 1:2K<\/p>\n\n\n\n<p>\u7b2c\u4e09\u5757\u8003\u67d3\u3001\u6253\u8d28\u8c31DIA-MS: Marker(plus), APEX2-V5 Elute 20\u03bcL(rep1, rep2), APEX2-V5-RLIG1 Elute 20\u03bcL(rep1, rep2), RLIG1-V5-APEX2 Elute 20\u03bcL(rep1, rep2), Marker<\/p>\n\n\n\n<p>\u4e0b\u5348\u9001\u4e86\u8d28\u8c31\u3002<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.4.5<\/h4>\n\n\n\n<p>18:00\u52a0\u836fdTAG\uff0c\u7a00\u91ca5000\u500d\u3002\u4e4b\u540e48h\u6ca1\u6709\u6362\u6db2\uff0c\u4e5f\u6ca1\u6709\u5728\u663e\u5fae\u955c\u4e0b\u89c2\u5bdf\u3002<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.4.7<\/h4>\n\n\n\n<p>19:00\u67e5\u770bSRP9\u548cSRP14\u5355\u514b\u9686\u3002\u53d1\u73b0\u90e8\u5206\u7ec6\u80de\u6b7b\u4ea1\u3002<\/p>\n\n\n\n<p>\u4e0d\u52a0\u836f\u677f1\uff1aSRP14C-54#\u6b7b\u4ea1<\/p>\n\n\n\n<p>\u4e0d\u52a0\u836f\u677f2\uff1aSRP14C-54#\u6b7b\u4ea1<\/p>\n\n\n\n<p>\u52a0dTAG 48h\u677f1\uff1aSRP14C-37,42,54,55\u6b7b\u4ea1<\/p>\n\n\n\n<p>\u52a0dTAG 48h\u677f2\uff1aSRP9C-34, SRP14C-37, 42, 44, 54, 55\u6b7b\u4ea1<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p>RNA-seq\u505a\u4e86SRP9C-5,9,22,28,34\u548cSRP14C-44\u7684\u300212-well\u5b54\u4e2d\u5f03\u6389\u57f9\u517b\u57fa\u76f4\u63a5\u52a0300\u03bcL TRIzol Reagent\uff0c\u6536\u96c6\u5230-20\u2103\u6682\u5b58\u3002<\/p>\n\n\n\n<p>RIP-seq\u505a\u4e86SRP9C-5,9,22,28\u6df7\u3002<\/p>\n\n\n\n<p>SRP14-37,42,44,55\u91cd\u65b0\u94fa\u677f\uff08\u6bcf\u4e2a12-well\u5b540.2million\u7ec6\u80de\uff09\uff0c\u51c6\u5907\u91cd\u65b0\u505aRIP-seq\u548cRNA-seq.<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p>\u6bcf\u4e2a\u6837\u54c1\u90fd\u662f40\u03bcL Dyna ProteinG beads\uff0c\u75285\u03bcl anti-SRP9(proteinTech)\u6216anti-HA(CST, C29F4)\u3002\u6bcf\u4e2a\u6837\u54c14\u767e\u4e07\u7ec6\u80de\u3002<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>SRP9C no-dTAG<\/td><td>SRP9C +dTAG 48h<\/td><\/tr><tr><td>input 3\u03bcl<\/td><td>input 3\u03bcl<\/td><\/tr><tr><td>anti-SRP9 replicate1<\/td><td>anti-SRP9 replicate1<\/td><\/tr><tr><td>anti-SRP9 replicate2(beads\u6297\u4f53\u53ea\u5305\u4e8630min\uff09<\/td><td>anti-SRP9 replicate2 (beads\u6297\u4f53\u53ea\u5305\u4e8630min)<\/td><\/tr><tr><td>anti-HA replicate1<\/td><td>anti-HA replicate1<\/td><\/tr><tr><td>anti-HA replicate2<\/td><td>anti-HA replicate2<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>4\u2103 binding 23:00~3:00 (4h\uff09<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.4.9<\/h4>\n\n\n\n<p>18:00\u7ed9SRP14\u5355\u514b\u9686\u52a0dTAG\u3002<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.4.10<\/h4>\n\n\n\n<p>22:00\u5de6\u53f3\u89c2\u5bdfSRP14\u5355\u514b\u9686\uff0c\u53d1\u73b0\u5df2\u7ecf\u957f\u5f97\u7206\u6ee1\u4e86\uff0c\u8d76\u7d27\u4f20\u4ee3\u3002\u52a0\u836f\u7684\u7ee7\u7eed\u52a0\u836f\uff0c\u4e0d\u52a0\u836f\u7684\u7ee7\u7eed\u4e0d\u52a0\u836f\u3002<\/p>\n\n\n\n<p>SRP9\u7684RIP-seq\u5efa\u5e93\u505a\u5230\u672b\u7aef\u4fee\u590d\u5b8c\u6210\u4e86\u3002\u57284\u2103\u653e\u7740\u7b494\u670812\u53f7\u7ee7\u7eed\u505a\u3002<\/p>\n\n\n\n<p>\u590d\u82cf\u4e86ANKRD11-N-APEX2-V5-pGEMT\u548cTetOn-APEX2-V5\u7ec6\u80de\u7cfb\u52306-well plate\uff0c\u6253\u7b97\u957f\u8d77\u6765\u505aAPEX2 labeling.<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.4.14<\/h4>\n\n\n\n<p>\u7a81\u7136\u53d1\u73b0\u6211\u7684\u6446\u70c2\u65e5\u8bb0\u600e\u4e48\u53d8\u6210\u5b9e\u9a8c\u8bb0\u5f55\u4e86\u3002\u4e0d\u884c\uff0c\u5b83\u5e94\u8be5\u8bb0\u5f55\u6211\u7684\u6574\u4e2a<s>\u6446\u70c2<\/s>\u751f\u6d3b\u3002<\/p>\n\n\n\n<p>\u6628\u5929\u662f\u5468\u516d\uff0c\u53bb\u4e00\u5929\u81ea\u7136\u5212\u4e86\u8239\u3002\u665a\u4e0a9\u70b9\u5c31\u5165\u7761\u4e86\uff0c\u4f46\u662f\u4e24\u70b9\u5c31\u9192\u4e86\uff0c\u7136\u540e\u73a9\u624b\u673a\u5230\u4e03\u70b9\u534a\u3002\u53bb\u5b9e\u9a8c\u5ba4\u6574\u7406\u4e86\u9f99\u821f\u961f\u4e0a\u4e2a\u6708\u7684\u7167\u7247\uff0c\u53d1\u4e86\u51e0\u7bc7\u4ece\u516c\u4f17\u53f7\u8f6c\u7684\u535a\u6587\u3002\u9001\u6837\u4e86\u5409\u56e0\u52a0\u7684\u6d4b\u5e8f\u3002<\/p>\n\n\n\n<p>10:34\uff0c\u6253\u7b97\u5f00\u59cb\u6574\u7406\u4e00\u4e0b\u5b9e\u9a8c\u8ba1\u5212\u3002\u6211\u9a6c\u4e0a\u8981\u535a\u8d44\u8003\uff0c\u597d\u70e6\u3002\u4ec0\u4e48\u90fd\u6ca1\u5f00\u59cb\u51c6\u5907\u3002<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.4.26<\/h4>\n\n\n\n<p>\u4eca\u592924\u70b9\u622a\u6b62\u63d0\u4ea4\u535a\u8d44\u8003\u7684proposal\uff0c\u6211\u53c8\u9677\u5165\u4e86 \u4e00\u8fb9\u7126\u8651 \u4e00\u8fb9\u6446\u70c2 \u7684\u60c5\u7eea\u602a\u5708\u4e2d\u3002I must tell myself again and again. Don&#8217;t stop. No matter what am I thinking about. Don&#8217;t stop on actions.<\/p>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.4.28<\/h4>\n\n\n\n<p>\u6628\u5929\u548c\u4eca\u5929\u662f\u6821\u5e86\u65e5\u3002\u81ea\u5f3a\u5e08\u5144\u662f\u6211\u7684\u535a\u8d44\u8003\u79d8\u4e66\uff0c\u56e0\u4e3a\u6211\u6ca1\u6709\u6309\u65f6\u63d0\u4ea4proposal\uff0c\u4ed6\u8bf4\u6700\u540e\u4e00\u6b21\u7684\u622a\u6b62\u671f\u9650\u662f\u5468\u4e00\uff08\u4e5f\u5c31\u662f\u660e\u5929\uff09\u3002\u4f46\u662f\u4eca\u5929\u6211\u53c8\u8e0f\u8e0f\u5b9e\u5b9e\u5728\u5e8a\u4e0a\u8eba\u4e86\u4e00\u6574\u5929\u3002\u6628\u5929\u665a\u4e0a\u5f00\u59cb\u54e6\u71ac\u591c\uff0c\u65e9\u4e0a\u516d\u70b9\u624d\u653e\u4e0b\u624b\u673a\u7761\u89c9\uff0c\u7136\u540e\u5341\u4e8c\u70b9\u9192\u4e86\u53c8\u5f00\u59cb\u5237\u624b\u673a\uff0c\u4e00\u76f4\u5230\u4e0b\u5348\u516d\u70b9\uff0c\u53bb\u6e05\u9752\u5feb\u9910\u5403\u4e86\u4eca\u5929\u552f\u4e00\u4e00\u987f\u996d\u3002\u7136\u540e\u5728\u5bbf\u820d\u6574\u7406\u4e86\u4e00\u4e0b\u5185\u52a1\u3002\u628a\u8bb8\u4e45\u6ca1\u7528\u8fc7\u7684\u684c\u5b50\u548c\u6905\u5b50\u6e05\u7406\u4e86\u4e00\u4e0b\u3002\u5fc3\u8840\u6765\u6f6e\u4e70\u4e86\u4e2a\u53f0\u5f0f\u673a\u3002\u56e0\u4e3a\u6211\u6628\u665a\u5728\u8bbe\u7f6e\u535a\u5ba2\u7684css\uff0c\u4f46\u662f\u56e0\u4e3a\u53ea\u6709\u624b\u673a\uff0c\u624b\u673a\u4e0a\u4e0d\u80fd\u6309f12\uff0c\u6240\u4ee5\u6d88\u8017\u4e86\u5927\u91cf\u7684\u65f6\u95f4\u3002\u6211\u8ba4\u4e3a\u5835\u4e0d\u5982\u758f\uff0c\u65e2\u7136\u81ea\u5df1\u771f\u7684\u6709\u8fd9\u4e2a\u7231\u597d\uff0c\u90a3\u5c31\u4e3a\u8fd9\u4e2a\u7231\u597d\u521b\u9020\u6761\u4ef6\uff0c\u800c\u4e0d\u662f\u5f3a\u8feb\u81ea\u5df1\u653e\u4e0b\u8fd9\u4e2a\u7231\u597d\u53bb\u641e\u79d1\u7814\u3002\u9009\u4e86\u4eac\u4e1c\u5feb\u9012\uff0c\u4eca\u592921\u70b9\u4e0b\u7684\u5355\uff0c\u660e\u65e99\uff5e11\u70b9\u9001\u8fbe\u5bbf\u820d\u3002\u3010\u8054\u60f3\u5929\u9038510s i5-13400\u3011\u7b97\u4e0a\u4e00\u4e2a\u5f88\u57fa\u7840\u7684\u663e\u793a\u5668\u4e00\u5171\u56db\u5343\u5757\u3002<\/p>\n\n\n\n<p>\u8fd8\u6709\u4e00\u4e2a\u6709\u8da3\u7684\u4e8b\u60c5\uff0c\u6211\u53d1\u73b0\u6211\u7ed9ipad\u914d\u7684\u5999\u63a7\u952e\u76d8\uff08\u5e73\u66ff\u7248\uff09\u7adf\u7136\u4e5f\u53ef\u4ee5\u76f4\u63a5\u8fde\u63a5\u624b\u673a\uff0c\u8fd9\u6837\u6253\u5b57\u5c31\u65b9\u4fbf\u592a\u591a\u4e86\u3002\u6211\u4e8e\u662f\u53ef\u4ee5\u5750\u5728\u6211\u5bbf\u820d\u6536\u62fe\u597d\u7684\u684c\u6905\u3001\u7528\u84dd\u7259\u8fde\u63a5\u7684\u5999\u63a7\u952e\u76d8\uff0c\u5728\u624b\u673a\u4e0a\u5199\u201c\u6446\u70c2\u65e5\u8bb0\u201d\uff0c\u4f53\u9a8c\u975e\u5e38\u597d\uff0c\u8fd9\u662f\u6211\u4eca\u5929\u6700\u5f00\u5fc3\u7684\u4e8b\u60c5\u3002<\/p>\n\n\n\n\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-constrained wp-block-group-is-layout-constrained\">\n<h4 class=\"wp-block-heading\">2024.5.6<\/h4>\n\n\n\n<p>\u4e94\u4e00\u5047\u671f\u7ed3\u675f\u4e86\u3002\u590d\u76d8\u4e00\u4e0b\uff0c5.1~5.2\u5728\u8ddf\u4e00\u4e2a\u79e6\u4e3a\u8bfe\u9898\u7ec4\u7684\u5e08\u59d0\u4e00\u8d77\u505aRIP-seq\u7684\u5efa\u5e93\u30025.3\u5728\u5bbf\u820d\u7761\u4e86\u4e00\u5929\uff0c5.3\u7684\u665a\u4e0a\u6b63\u5728\u5bbf\u820d\u73a9MC\u7684\u65f6\u5019\u8001\u677f\u95ee\u4e86<a href=\"http:\/\/localhost\/?p=3388\" title=\"TypeIII CRISPR\">TypeIII CRISPR<\/a>\u7684\u8fdb\u5c55\u3002\u7136\u540e\u8ba9\u4e0a\u6d77\u79d1\u6280\u5927\u5b66\u7684\u6c60\u5929\u6559\u6388\u7ec4\u7684\u9a6c\u6052\u6e90\u5e08\u5144\u5bc4\u8fc7\u6765\u4e86\u8d28\u7c92\u30025.4\u4e5f\u7761\u4e86\u4e00\u5929\uff0c\u7136\u540e\u665a\u4e0a\u53bbIBP\u8ddf\u7696\u8c6b\u3001\u4f73\u6862\u548c\u8bd7\u701a\u53bb\u65b0\u5965\u8d2d\u7269\u4e2d\u5fc3\u5403\u4e86\u725b\u8169\u9505\u30025.5\u9664\u4e86\u5212\u4e86\u4e00\u4e0b\u5348\u8239\u4e5f\u7b97\u653e\u5047\u4e86\u3002\u7136\u540e\u4eca\u5929\uff0c5.6\uff0c\u65e9\u4e0a\u7761\u523011\u70b9\uff0c\u7b80\u5355\u5403\u4e86\u5348\u996d\u7136\u540e\u53bb\u5317\u4f53\u4e0a\u4e86Yoga\u56e2\u8bfe\u3002\u524d\u4e00\u5929\u665a\u4e0a<a href=\"http:\/\/localhost\/?p=1889\" title=\"\u5f90\u6657\u827a\">hanyi<\/a>\u8fd8\u63d0\u9192\u6211\u4e0d\u8981\u71ac\u591c\u8bb0\u5f97\u53c2\u52a0\uff0c\u4e4b\u524d\u5df2\u7ecf\u9e3d\u4e86\u4e24\u6b21\u4e86\u3002\u611f\u89c9\u5f88\u597d\u73a9\u3002\u7136\u540e\u53c8\u56de\u5bdd\u5ba4\u7761\u4e86\u4e00\u89c9\uff0c\u9192\u6765\u5df2\u7ecf\u662f\u4e0b\u534817\u70b9\u591a\u4e86\u3002\u60f3\u8d77\u6765\u4eca\u5929\u7684\u4e8b\u60c5\u8fd8\u90fd\u6ca1\u5f00\u59cb\u505a\u3002\u8d76\u7d27\u76d8\u70b9\u4e00\u4e0b\u3002<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u6574\u7406\u6e56\u5dde\u6bd4\u8d5b\u7684\u62a5\u540d\u4fe1\u606f\uff0c\u627e\u51af\u8001\u5e08\u7b7e\u5b57\u548c\u4f53\u80b2\u90e8\u76d6\u7ae0\uff08\u8fd9\u4e2a\u672c\u6765\u6253\u7b97\u4eca\u5929\u505a\uff0c\u622a\u6b62\u65e5\u671f\u4e5f\u662f\u4eca\u5929\uff0c\u4f46\u662f\u73b0\u5728\u8001\u5e08\u4eec\u5df2\u7ecf\u4e0b\u73ed\u4e86\uff0c\u8fd8\u662f\u660e\u5929\u5f04\u5427\uff09<\/li>\n\n\n\n<li>\u8ddf\u8001\u677f\u8bf4\u4e86\u4eca\u5929\u8981\u7ed9\u4ed6\u770b\u8bbe\u8ba1\u7684TypeIII CRISPR\u7684\u65b9\u6848\uff0c\u6253\u7b97\u73b0\u5728\u7acb\u523b\u505a\u3002<\/li>\n\n\n\n<li>\u8001\u677f\u8bf4\u5411ASW dTAG\u7ec6\u80de\u7cfb\u5bfc\u5165Cas13d\u7684\u53ef\u4ee5\u5f00\u59cb\u4e86\uff0c\u4f46\u6211\u8fd8\u6ca1\u505a\u57fa\u56e0\u7ec4\u548cWB\u9a8c\u8bc1\u3002<\/li>\n\n\n\n<li>\u63d0SRP9\/14\u7684RNA\u5e76rRNA depletion\u5efa\u5e93\u3002<\/li>\n\n\n\n<li>\u5468\u4e09\u8981work report\u4e86\uff0c\u6211\u9700\u8981\u5728\u90a3\u4e4b\u524d\u518d\u6316\u6398L1 RNA pull down\u548cRLIG1-dTAG\u7684\u8d28\u8c31\u6570\u636e\u3002<\/li>\n<\/ol>\n\n\n\n<p>OK\u90a3\u6211\u73b0\u5728\u5f00\u59cb\u7b2c\u4e8c\u9879\u4e86\u3002\u8001\u677f\u63d0\u5230\u4e86\u51e0\u4e2a\u91cd\u70b9\u95ee\u9898\u8981\u9010\u4e2a\u56de\u7b54\u4e00\u4e0b\u3002<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<ol class=\"wp-block-list\">\n<li>\u6536\u5230\u8d28\u7c92\uff0c\u6253\u7b97\u505a\u4ec0\u4e48<\/li>\n\n\n\n<li>\u80fd\u5426\u50cf cas13d sgRNA\u7cfb\u7edf\u4e00\u6837\uff0c\u505a\u4e2a\u7a33\u8f6c\u7ec6\u80de\u7cfb<\/li>\n\n\n\n<li>\u8fd9\u4e2a\u8d28\u7c92\u4e2d\u7684\u5e8f\u5217 \u9f50\u5168\u5417\uff1f\u8fd8\u9700\u8981\u5408\u6210DNA\u7247\u6bb5\uff0c\u51d1\u9f50\u5417<\/li>\n<\/ol>\n\n\n\n<p>\u8fd8\u63d0\u4e86\u51e0\u4e2a\u5efa\u8bae\uff1a<\/p>\n\n\n\n<ol 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