{"id":8030,"date":"2024-12-18T17:49:57","date_gmt":"2024-12-18T09:49:57","guid":{"rendered":"https:\/\/fanyiming.life\/?p=8030"},"modified":"2025-12-21T01:04:05","modified_gmt":"2025-12-20T17:04:05","slug":"%e7%bb%8f%e5%85%b8rna-ligase","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=8030","title":{"rendered":"\u7ecf\u5178RNA ligase"},"content":{"rendered":"\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>Classic RNA and DNA ligases share a core adenylyltransferase domain and an active site composed of conserved amino acid sequence motifs. <strong>Classic RNA ligases can be divided into two main classes: the Rnl1 family and the Rnl2 family. <\/strong>Rnl2 ligases, like DNA ligases, prefer to seal 3\u2032-OH\/5\u2032-PO<sub>4<\/sub>\u00a0nicks in double-stranded substrates. By contrast, Rnl1 ligases prefer to join single-stranded ends, especially within stem-loop structures. The reported substrate preference for RtcB is similar to that of Rnl1. However, the crystal structures of RtcB revealed a novel fold and active site (<a href=\"https:\/\/www.pnas.org\/doi\/10.1073\/pnas.1320033110?url_ver=Z39.88-2003&amp;rfr_id=ori%3Arid%3Acrossref.org&amp;rfr_dat=cr_pub++0pubmed#core-r12\">12<\/a>\u2013<a href=\"https:\/\/www.pnas.org\/doi\/10.1073\/pnas.1320033110?url_ver=Z39.88-2003&amp;rfr_id=ori%3Arid%3Acrossref.org&amp;rfr_dat=cr_pub++0pubmed#core-r14\">14<\/a>), reflecting the uniqueness of RtcB in terms of its biochemical mechanism.<\/p>\n<cite><a href=\"https:\/\/www.pnas.org\/doi\/10.1073\/pnas.1320033110?url_ver=Z39.88-2003&amp;rfr_id=ori%3Arid%3Acrossref.org&amp;rfr_dat=cr_pub++0pubmed\">Cross-over of RNA 3\u2032-phosphate ligase into the DNA world | PNAS<\/a><\/cite><\/blockquote>\n\n\n\n<p>\u7ecf\u5178\u7684RNA\u548cDNA\u8fde\u63a5\u9176\u5171\u4eab\u4e00\u4e2a\u6838\u5fc3\u817a\u82f7\u57fa\u8f6c\u79fb\u9176\u7ed3\u6784\u57df\u548c\u4e00\u4e2a\u7531\u4fdd\u5b88\u7684\u6c28\u57fa\u9178\u5e8f\u5217\u57fa\u5e8f\u7ec4\u6210\u7684\u6d3b\u6027\u4f4d\u70b9\u3002\u7ecf\u5178\u7684RNA\u8fde\u63a5\u9176\u53ef\u5206\u4e3a\u4e24\u5927\u7c7b\uff1aRnl1\u5bb6\u65cf\u548cRnl2\u5bb6\u65cf\u3002Rnl2\u8fde\u63a5\u9176\u4e0eDNA\u8fde\u63a5\u9176\u4e00\u6837\uff0c\u66f4\u503e\u5411\u4e8e\u5c01\u95ed\u53cc\u94fe\u5e95\u7269\u4e2d\u76843 &#8216; -OH\/5 &#8216; -PO\u00a0<sub>4<\/sub>\u00a0\u7f3a\u53e3\u3002\u76f8\u6bd4\u4e4b\u4e0b\uff0cRnl1\u8fde\u63a5\u9176\u66f4\u503e\u5411\u4e8e\u8fde\u63a5\u5355\u94fe\u672b\u7aef\uff0c\u7279\u522b\u662f\u5728\u830e\u73af\u7ed3\u6784\u4e2d\u3002RtcB\u5bf9\u5e95\u7269\u7684\u504f\u597d\u4e0eRnl1\u76f8\u4f3c\u3002\u7136\u800c\uff0cRtcB\u7684\u6676\u4f53\u7ed3\u6784\u663e\u793a\u51fa\u65b0\u7684\u8936\u76b1\u548c\u6d3b\u6027\u4f4d\u70b9\uff0812-14\uff09\uff0c\u53cd\u6620\u4e86RtcB\u5728\u751f\u5316\u673a\u5236\u4e0a\u7684\u72ec\u7279\u6027\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd9\u7bc7\u6587\u732e\u7efc\u8ff0\u805a\u7126\u4e8e\u7ecf\u5178RNA\u8fde\u63a5\u9176\uff0c\u7cfb\u7edf\u6bd4\u8f83\u4e86Rnl1\u4e0eRnl2\u4e24\u5927\u6838\u5fc3\u5bb6\u65cf\u5728\u5e95\u7269\u504f\u597d\uff08\u5355\u94fe\/\u53cc\u94fe\uff09\u4e0e\u50ac\u5316\u673a\u5236\u4e0a\u7684\u5173\u952e\u5dee\u5f02\uff0c\u5e76\u7279\u522b\u63a2\u8ba8\u4e86RtcB\u8fde\u63a5\u9176\u5728\u7ed3\u6784\u4e0e\u529f\u80fd\u4e0a\u7684\u72ec\u7279\u6027\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-8030","post","type-post","status-publish","format-standard","hentry","category-18"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/8030","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8030"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/8030\/revisions"}],"predecessor-version":[{"id":13891,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/8030\/revisions\/13891"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8030"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8030"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8030"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}