\u7b2c\u4e00\u4e2a\u662f2015\u5e74\u7528Ecoli RTCB\u5728yeast\u4e2d\u4f53\u5916\u7b5b\u90095′-OH\u672b\u7aefRNA.<\/p>\n\n\n\n
Global analysis of RNA cleavage by 5\u2032-hydroxyl RNA sequencing | Nucleic Acids Research | Oxford Academic<\/a><\/p>\n\n\n\n \u7b2c\u4e8c\u4e2a\u662f2019\u5e74\u5728mouse issue\u7b5b\u90092’3-cP\u672b\u7aefRNA.<\/p>\n\n\n\n Genome-wide identification of short 2\u2032,3\u2032-cyclic phosphate-containing RNAs and their regulation in aging | PLOS Genetics<\/a><\/p>\n\n\n\n \u8ba8\u8bba\u90e8\u5206\u6bd4\u8f83\u6709\u8da3\uff1a<\/p>\n\n\n\n In this study, we unraveled a previously hidden layer of transcriptome by comprehensively identifying short cP-RNA species expressed in mouse tissues. Each cP-RNA library, obtained by cP-RNA-seq, commonly comprised previously uninterrogated cP-RNA species that are expected to be generated by specific RNA cleavage between cytidine and adenosine (S11 Fig<\/a><\/strong>). The endoribonuclease generating the identified cP-RNAs is unknown but should possess highly specific recognition and cleavage activity for the 5\u2032-CA-3\u2032 sequence. ANG might be the candidate enzyme because ANG produces cP-RNAs [10<\/a>,28<\/a>] and shows the highest activity toward the 5\u2032-CA-3\u2032 among four examined dinucleotides in a previous study [29<\/a>]. Among other cP-generating ribonucleases, GCN4 shows high cleavage activity between C and A [30<\/a>]. Because cP-RNAs are not evenly produced from overall sequences of substrate RNAs but rather derived from specific sites, the RNA cleavage activity to generate cP-RNAs might be exquisitely regulated. Further investigations are required to clarify the enzyme and its activity for the generation of the cP-RNAs identified in this study. Considering the already-proven functional significance of tRNA-derived cP-RNAs [6<\/a>,7<\/a>,8<\/a>,9<\/a>,10<\/a>], it is not surprising that the identified cP-RNAs themselves have functional significance. Their abundance and expression from only specific sites of substrate RNAs might also imply their expression as functional molecules. The reduction of cP-RNA generation through aging implied its physiological relevance. It will be intriguing to examine further the cellular factors that influence, or are influenced by, the expression of cP-RNAs and their underlying molecular mechanisms, which will define cP-RNAs as an abundant class of functional non-coding RNAs.<\/p>\n<\/blockquote>\n\n\n\n \u53ef\u4ee5\u5b66\u4e60\u7684\u70b9\u662f\u5176\u4e2d\u4e24\u4e2acandidate endoribonuclease: ANG, GCN4.<\/p>\n\n\n\n \u4ee5\u53ca\u63d0\u4f9b\u4e86RTCB\u4f5c\u4e3aRNA ligase\u7684\u9009\u62e9\u6027\u6765\u6e90\u4e4b\u4e00\u53ef\u80fd\u662f\u67d0\u79cd\u6838\u7cd6\u6838\u9178\u5185\u5207\u9176\u7684\u5e95\u7269\u7279\u5f02\u6027\uff1aBecause cP-RNAs are not evenly produced from overall sequences of substrate RNAs but rather derived from specific sites, the RNA cleavage activity to generate cP-RNAs might be exquisitely regulated.<\/strong><\/p>\n\n\n\n \u4ee5\u53ca\u8fd9\u65b9\u9762\u7684\u540e\u7eed\u7814\u7a76\u610f\u4e49\uff0c\u53ef\u80fd\u63d0\u4f9b\u4e00\u79cd\u5168\u65b0\u7684\u6709\u529f\u80fd\u7684non-coding RNA\u2014cP-RNAs.<\/p>\n","protected":false},"excerpt":{"rendered":" \u672c\u6587\u56de\u987e\u4e86\u4e24\u9879\u7279\u6b8a\u7684RNA\u672b\u7aef\u9274\u5b9a\u7814\u7a76\uff1a2015\u5e74\u5229\u7528\u5927\u80a0\u6746\u83ccRTCB\u5728\u9175\u6bcd\u4e2d\u4f53\u5916\u7b5b\u90095′-OH\u672b\u7aefRNA\uff0c\u4ee5\u53ca2019\u5e74\u5728\u5c0f\u9f20\u7ec4\u7ec7\u4e2d\u9274\u5b9a2′,3′-\u73af\u78f7\u9178(cP)\u672b\u7aefRNA\u3002\u540e\u8005\u63ed\u793a\u4e86cP-RNA\u53ef\u80fd\u7531ANG\u6216GCN4\u7b49\u5185\u5207\u9176\u7279\u5f02\u6027\u4ea7\u751f\uff0c\u5e76\u63d0\u793a\u5176\u4f5c\u4e3a\u4e00\u79cd\u53d7\u8c03\u63a7\u3001\u5177\u6709\u6f5c\u5728\u529f\u80fd\u7684\u65b0\u578b\u975e\u7f16\u7801RNA\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-8048","post","type-post","status-publish","format-standard","hentry","category-18"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/8048","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8048"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/8048\/revisions"}],"predecessor-version":[{"id":13889,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/8048\/revisions\/13889"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8048"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8048"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8048"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}\n