{"id":8255,"date":"2024-12-30T17:30:55","date_gmt":"2024-12-30T09:30:55","guid":{"rendered":"https:\/\/fanyiming.life\/?p=8255"},"modified":"2025-12-21T01:04:02","modified_gmt":"2025-12-20T17:04:02","slug":"%e7%bb%86%e8%83%9e%e9%93%ba%e6%9d%bf%e5%af%86%e5%ba%a6","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=8255","title":{"rendered":"\u7ec6\u80de\u94fa\u677f\u5bc6\u5ea6"},"content":{"rendered":"\n<p>\u5b9e\u9a8c\u5ba4\u7684\u8d85\u51c0\u5de5\u4f5c\u53f0\u4e0a\u9762\u4e00\u76f4\u8d34\u7740\u4e00\u5f20\u53e4\u65e9\u7684\u5404\u57f9\u517b\u5668\u76bf\u5bf9\u5e94\u7ec6\u80de\u91cf\u7684\u56fe\u7eb8\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"588\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/12\/20241230172701866-1024x588.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-8258\" style=\"width:786px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/12\/20241230172701866-1024x588.png?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/12\/20241230172701866-300x172.png?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/12\/20241230172701866-768x441.png?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/12\/20241230172701866-1536x883.png?x-oss-process=style\/default 1536w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2024\/12\/20241230172701866-2048x1177.png?x-oss-process=style\/default 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>\u6211\u4e00\u76f4\u8ba4\u4e3a\uff0c\u6700\u540e\u90a3\u4e00\u5217\u662f100% confluency\u65f6\u7684\u7ec6\u80de\u91cf\uff0c\u4f46\u5b9e\u9645\u4e0a\u7ec6\u80de\u7206\u6ee1\u7684\u65f6\u5019\u8fdc\u8d85100% confluency\uff0c\u6bd4\u5982\u6628\u5929\u4f20HEK293T\uff0c\u57286-well plate\u957f\u81f3\u7206\u6ee1\uff0c\u8ba1\u6570\u53d1\u73b0\u4e00\u4e2a\u5b54\u7684\u7ec6\u80de\u91cf\u662f5.8&#215;10^6\uff0c\u8fdc\u8d85\u8868\u91cc\u5199\u76842.5&#215;10^6. <\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>\u5728\u517b\u6ca1\u6709HEK293T\u5206\u88c2\u8fd9\u4e48\u5feb\u7684NCCIT\u65f6\uff0c\u4e5f\u53d1\u73b0\u7206\u6ee1\u65f6\u8fdc\u8d852.5million\uff0c\u6700\u591a\u65f6\u9690\u7ea6\u8bb0\u5f97\u67094million.<\/p>\n<\/blockquote>\n\n\n\n<p>\u8fd9\u6b21\u6211\u7528HEK293T\u6765\u5305\u75c5\u6bd2\uff0c\u9700\u8981\u94fa\u7684\u5bc6\u4e00\u70b9\uff0c\u4e8e\u662f1\u4f202\uff0c\u57286-well 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confluency.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd9\u7bc7\u7b14\u8bb0\u8bb0\u5f55\u4e86\u4f5c\u8005\u901a\u8fc7\u5b9e\u9a8c\u9a8c\u8bc1HEK293T\u7ec6\u80de\u57286\u5b54\u677f\u4e2d\u7684\u5b9e\u9645\u94fa\u677f\u5bc6\u5ea6\uff0c\u53d1\u73b0\u5b9e\u9a8c\u5ba4\u5e38\u7528\u53c2\u8003\u6570\u636e\u5b58\u5728\u504f\u5dee\uff0c\u5e76\u63d0\u4f9b\u4e86\u66f4\u51c6\u786e\u7684100%\u6c47\u5408\u5ea6\u7ec6\u80de\u91cf\u53ca\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u94fa\u677f\u7ecf\u9a8c\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-8255","post","type-post","status-publish","format-standard","hentry","category-note"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/8255","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=8255"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/8255\/revisions"}],"predecessor-version":[{"id":13885,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/8255\/revisions\/13885"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=8255"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=8255"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=8255"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}