{"id":9583,"date":"2025-03-20T01:17:49","date_gmt":"2025-03-19T17:17:49","guid":{"rendered":"https:\/\/fanyiming.life\/?p=9583"},"modified":"2025-12-21T01:03:56","modified_gmt":"2025-12-20T17:03:56","slug":"%e6%b5%8b%e5%ba%8f%e4%b8%ad%e7%9a%84y%e5%9e%8b%e6%8e%a5%e5%a4%b4","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=9583","title":{"rendered":"\u6d4b\u5e8f\u4e2d\u7684Y\u578b\u63a5\u5934"},"content":{"rendered":"\n<p>\u8fd9\u5929\u5bf9\u7740\u4e0b\u9762\u7684\u5efa\u5e93\u539f\u7406\u56fe\u6df1\u6df1\u53d1\u5446\uff0c\u5ffd\u7136\u53d1\u73b0Truncated 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height=\"834\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/03\/20250320010225926.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-9584\" style=\"width:562px;height:auto\" title=\"\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/03\/20250320010225926.png?x-oss-process=style\/default 741w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/03\/20250320010225926-267x300.png?x-oss-process=style\/default 267w\" sizes=\"auto, (max-width: 741px) 100vw, 741px\" \/><\/figure>\n\n\n\n<p>\u8ddf\u5b9e\u9a8c\u5ba4\u5c0f\u4f19\u4f34\u8ba8\u8bba\u534a\u5929\uff0c\u5176\u4ed6\u4eba\u4e5f\u89c9\u5f97\u5947\u602a\uff0c\u9042\u4e0a\u7f51\u4e00\u67e5\uff0c\u770b\u5230\u4e00\u4e2a\u201c<a href=\"https:\/\/www.jianshu.com\/p\/4aded8c2d368\">Y\u578b\u63a5\u5934<\/a>\u201d\u5b57\u773c\uff0c\u8c41\u7136\u5f00\u6717\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"753\" height=\"130\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/03\/20250320010716885.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-9585\" style=\"width:733px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/03\/20250320010716885.png?x-oss-process=style\/default 753w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/03\/20250320010716885-300x52.png?x-oss-process=style\/default 300w\" sizes=\"auto, (max-width: 753px) 100vw, 753px\" \/><\/figure>\n\n\n\n<p>\u8fd9\u5c31\u8bf4\u6765\u60ed\u6127\u4e86\uff0c\u5efa\u4e86\u51e0\u5e74\u6587\u5e93\u4e86\uff0c\u539f\u7406\u6ca1\u641e\u61c2\uff0c\u57fa\u7840\u4e0d\u7262\u5730\u52a8\u5c71\u6447\u4e86\u3002<\/p>\n\n\n\n<p>\u4f46\u9664\u4e86\u6211\u7684\u95ee\u9898\u4e4b\u5916\uff0c\u8fd9\u4e2a<a href=\"#hairenbuqian\">Abclonal\u7684\u793a\u610f\u56fe<\/a>\u4e5f\u8d1f\u5f88\u5927\u8d23\u4efb\u3002\u4e0a\u9762\u7684Truncated Adapter\u6839\u672c\u4e0d\u662fY\u578b\uff0c\u800c\u662f\u5b8c\u7f8e\u4e92\u8865\u914d\u5bf9\u3002\u90a3\u662f\u4e0d\u662f\u56e0\u4e3a\u5b83\u201ctruncated\u201d\u4e86\u6240\u4ee5\u4e0d\u662fY\u4e86\u5462\uff1f\u53ea\u4fdd\u7559\u4e86\u5b8c\u5168\u914d\u5bf9\u7684\u90a3\u90e8\u5206\uff1f\u7167\u7740\u6d4b\u5e8f\u8ba2\u5355\u4e0a\u7684\u793a\u610f\u56fe\u6570\u4e86\u6570\u78b1\u57fa\u6570\uff0c\u53ea\u670940\u2103\u7684\u914d\u5bf9\u533a\u57df\uff0c\u800cPCR\u662f60\u2103\uff0c\u4e0d\u53ef\u80fd\u5c31\u8fd9\u4e48\u77ed\u5f15\u7269\u7ed3\u5408\u533a\u57df\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"872\" height=\"103\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/03\/20250320011019140.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-9586\" style=\"width:753px;height:auto\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/03\/20250320011019140.png?x-oss-process=style\/default 872w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/03\/20250320011019140-300x35.png?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/03\/20250320011019140-768x91.png?x-oss-process=style\/default 768w\" sizes=\"auto, (max-width: 872px) 100vw, 872px\" \/><\/figure>\n\n\n\n<pre class=\"wp-block-verse\">5\u2019-AATGATACGGCGACCACCGAGATCTACAC\uff08I5\uff09ACACTCTTTCCCTACACGACGCTCTTCCGATCT-{insert}-AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC\uff08I7\uff09ATCTCGTATGCCGTCTTCTGCTTG--3\u2019<\/pre>\n\n\n\n<p><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\" \/>\n\n\n\n<p>\u95eeAbclonal\u9500\u552e\u8981\u4e86Truncated Adapter\u5e8f\u5217\uff1a<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>\u77ed\u63a5\u5934\u7684Top\u94fe\uff1a<br>5-ACACTCTTTCCCTACACGACGCTCTTCCGATC-T-3<br>\u77ed\u63a5\u5934\u7684Bottom\u94fe\uff1a<br>5-GATCGGAAGAGCACACGTCTGAACTCCAGTC-3<\/p>\n<\/blockquote>\n\n\n\n<p>\u539f\u6765\u5982\u6b64\uff0c\u5b83\u5176\u5b9e\u4e5f\u662f\u4e2aY\uff0c\u53ea\u4e0d\u8fc7\u4e0a\u8fb9\u7684\u53c9\u77ed\u4e86\u4e00\u70b9\u3002<\/p>\n\n\n\n<pre class=\"wp-block-code\"><code>Top\u94fe\u5355\u81c2\uff085'\u21923'\u65b9\u5411\uff09\uff1a\n5'-ACACTCTTTCCCTACACG\n                     \\\n                      ACGCTCTTCCGATC-T-3'\n                      CACGAGAAGGCTAG-5'\n                     \/\n 3'-CTGACCTCAAGTCTGCA<\/code><\/pre>\n","protected":false},"excerpt":{"rendered":"<p>\u4f5c\u8005\u5728\u5ba1\u89c6\u5efa\u5e93\u539f\u7406\u56fe\u65f6\uff0c\u5bf9Truncated 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