{"id":9598,"date":"2025-03-20T13:03:26","date_gmt":"2025-03-20T05:03:26","guid":{"rendered":"https:\/\/fanyiming.life\/?p=9598"},"modified":"2025-12-21T01:03:53","modified_gmt":"2025-12-20T17:03:53","slug":"k562%e6%84%9f%e6%9f%93%e7%97%85%e6%af%92","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=9598","title":{"rendered":"K562\u611f\u67d3\u75c5\u6bd2"},"content":{"rendered":"\n

\u5305\u75c5\u6bd2<\/h2>\n\n\n\n
    \n
  1. \u63d0\u524d\u51c6\u5907\u597d\u8d28\u7c92\uff0c\u5e76\u901a\u8fc7\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u786e\u8ba4\u8d85\u87ba\u65cb\u6bd4\u4f8b\u6b63\u5e38\u3002\u5c06PMD2G\u548cPSAX2\u8d28\u7c92\u5206\u88c5\u81f3\u5c0f\u7ba1\u4e2d\uff0c\u6bcf\u6b21\u4f7f\u7528\u4e00\u7ba1\uff0c\u4ee5\u51cf\u5c11\u51bb\u878d\u6b21\u6570\u3002<\/li>\n\n\n\n
  2. \u572812\u5b54\u677f\u62166\u5b54\u677f\u4e2d\u94fa\u677fHEK293T\u7ec6\u80de\uff0c\u5206\u522b\u5411\u6bcf\u4e2a\u5b54\u4e2d\u52a0\u51651mL\u62162mL\u57f9\u517b\u57fa\u3002<\/li>\n\n\n\n
  3. \u57f9\u517bHEK293T\u7ec6\u80de\u81f390%\u6c47\u5408\u5ea6\uff0c\u540c\u65f6\u786e\u4fdd\u57f9\u517b\u57fa\u672a\u56e0\u957f\u65f6\u95f4\u4f7f\u7528\u800c\u53d8\u9ec4\u3002<\/li>\n\n\n\n
  4. \u4f7f\u7528Neofect\u8fdb\u884c\u8f6c\u67d3\u3002\u5bf9\u4e8e12\u5b54\u677f\uff0c\u6bcf\u4e2a\u6837\u54c1\u4f7f\u752890\u03bcL Opti-MEM\uff0c\u52a0\u51650.9\u03bcg\u7a7f\u68ad\u8d28\u7c92\u30010.3\u03bcg PMD2G\u548c0.6\u03bcg PSAX2\u8d28\u7c92\uff0c\u603b\u8ba11.8\u03bcg\u8d28\u7c92\uff0c\u7136\u540e\u52a0\u51651.8\u03bcL Neofect\u3002\uff08\u7ecf\u5e38\u6027\u5fd8\u8bb0\u52a0\u5305\u88c5\u8d28\u7c92\uff09<\/strong><\/em><\/li>\n\n\n\n
  5. \uff08\u53ef\u9009\uff09<\/em><\/strong>\u8f6c\u67d3\u540e12\u5c0f\u65f6\u66f4\u6362\u4e3a\u65b0\u9c9c\u57f9\u517b\u57fa\u3002\u5982\u679c\u7a7f\u68ad\u8d28\u7c92\u5e26\u6709\u8367\u5149\u6807\u8bb0\uff0c\u6b64\u65f6\u53ef\u5728\u8367\u5149\u663e\u5fae\u955c\u4e0b\u8fdb\u884c\u68c0\u6d4b\u3002<\/li>\n\n\n\n
  6. \u8f6c\u67d3\u540e48\u5c0f\u65f6\u6536\u96c6\u75c5\u6bd2\uff08\u57f9\u517b\u57fa\u4e0a\u6e05\uff09\u3002\u4f7f\u75280.22\u03bcM\u6ee4\u5668\u53bb\u9664\u7ec6\u80de\u788e\u7247\uff0c\u6216\u4ee54000rpm\u79bb\u5fc34\u5206\u949f\u540e\u8fc7\u6ee4\u7ec6\u80de\u788e\u7247\u3002\u5efa\u8bae\u63d0\u524d\u51c6\u5907\u597d\u5f85\u611f\u67d3\u7684\u7ec6\u80de\uff0c\u907f\u514d\u51b7\u51bb\uff1b\u82e5\u5fc5\u987b\u51b7\u51bb\uff0c\u8bf7\u4fdd\u5b58\u4e8e-80\u2103\u3002<\/li>\n<\/ol>\n\n\n\n

    \u611f\u67d3\u75c5\u6bd2<\/h2>\n\n\n\n
      \n
    1. \u63d0\u524d\u51c6\u5907\u597d\u5f85\u611f\u67d3\u7684K562\u7ec6\u80de\uff0c\u5e76\u5728\u611f\u67d3\u524d\u5c06\u5176\u94fa\u677f\u81f312\u5b54\u677f\u62166\u5b54\u677f\u4e2d\u3002<\/li>\n\n\n\n
    2. \u786e\u4fdd\u7ec6\u80de\u72b6\u6001\u826f\u597d\uff0c\u611f\u67d3\u75c5\u6bd2\u524d\u66f4\u6362\u65b0\u9c9c\u57f9\u517b\u57fa\u3002<\/li>\n\n\n\n
    3. 12\u5b54\u677f\u4e2d\u6bcf\u5b54\u63a5\u79cd0.5 million\u7ec6\u80de\uff0c6\u5b54\u677f\u4e2d\u6bcf\u5b54\u63a5\u79cd1 million\u7ec6\u80de\u3002<\/li>\n\n\n\n
    4. \u5c06\u75c5\u6bd2\u60ac\u6db2\u52a0\u5165\u57f9\u517b\u57fa\u4e2d\u3002\u5177\u4f53\u7ec6\u80de\u6570\u91cf\u4e0e\u75c5\u6bd2\u4f53\u79ef\u7684\u6bd4\u4f8b\u9700\u6839\u636e\u5b9e\u9a8c\u6761\u4ef6\u8fdb\u884c\u4f18\u5316\u3002\u9700\u8981\u6ce8\u610f\u7684\u662f\uff0c\u76f8\u540c\u4f53\u79ef\u7684\u75c5\u6bd2\u60ac\u6db2\u5728\u4e0d\u540c\u6279\u6b21\u4e2d\u53ef\u80fd\u5177\u6709\u4e0d\u540c\u7684\u75c5\u6bd2\u6ef4\u5ea6\u3002<\/li>\n\n\n\n
    5. \u5c06\u5b54\u677f\u4ee51000\u00d7g\u79bb\u5fc3\uff0c\u5ba4\u6e29\u6761\u4ef6\u4e0b\u79bb\u5fc330\u5206\u949f\u3002<\/li>\n\n\n\n
    6. \u79bb\u5fc3\u540e\u5c06\u5b54\u677f\u653e\u56de\u57f9\u517b\u7bb1\u4e2d\u7ee7\u7eed\u57f9\u517b\u3002<\/li>\n\n\n\n
    7. \u611f\u67d312\u5c0f\u65f6\u540e\u66f4\u6362\u65b0\u9c9c\u57f9\u517b\u57fa\u3002\uff08\u8fd9\u6b65\u5bb9\u6613\u5fd8\uff09<\/em><\/strong><\/li>\n\n\n\n
    8. \u611f\u67d3\u540e24\u5c0f\u65f6\uff0c\u52a0\u836f\u7269\u7b5b\u9009\uff08\u6216\u611f\u67d3\u540e48h\u8dd1FACS\u5bcc\u96c6\u8367\u5149\u9633\u6027\u7ec6\u80de\uff09\u3002<\/li>\n<\/ol>\n\n\n\n
      \n\n\n\n

      \uff01\u6ce8\u610f\uff1a\u4eb2\u6d4b\uff0c\u611f\u67d3\u540e24h\u548c48h\u540e\u8367\u5149\u8868\u8fbe\u6548\u7387\u5343\u5dee\u4e07\u522b\uff0c\u540c\u4e00\u6279\u611f\u67d3\u75c5\u6bd2\u7684K562\u7ec6\u80de\uff0c24h post-infection\u6536\u4e863700\u4e2a\u9633\u6027\u7ec6\u80de\uff0c\u800c48h\u7684\u8f7b\u677e\u78342\u4e07\uff0c\u8367\u5149\u5f3a\u5ea6\u4e5f\u663e\u8457\u63d0\u9ad8\u3002\u6240\u4ee5\u4e00\u5b9a\u4e0d\u8981\u90a3\u4e48\u65e9\u5bcc\u96c6\uff0c\u8840\u4e8f\uff01<\/p>\n\n\n\n

      \u554a\uff0c\u6709\u4e00\u4e2a\u66f4\u52a0\u79bb\u8c31\u7684\u6570\u636e\u503c\u5f97\u8bb0\u5f55\uff1a<\/p>\n\n\n\n

      \n

      \u53e6\u5916\uff0c\u53d1\u73b0\u8fd9\u6b21mCherry\u9633\u6027\u7387\u592a\u9ad8\u4e86\uff0c\u80fd\u8fbe\u523060%~80%\uff0c\u8fd9\u662f\u6211\u6700\u9ad8\u7684\u4e00\u6b21\uff0c\u4e4b\u524d20%\u6211\u90fd\u89c9\u5f97\u9ad8\u4e86\u3002\u8fd9\u6b21\u4e0d\u540c\u7684\u5730\u65b9\uff1a<\/p>\n\n\n\n

        \n
      1. \u5728HEK293T\u5305\u75c5\u6bd248h\u65f6\u6ca1\u6709\u53d7\u75c5\u6bd2\uff0c66h\u65f6\u624d\u6536\u3002<\/li>\n\n\n\n
      2. 800\u03bcL\u75c5\u6bd2\u611f\u67d3\u4e86\u7ea60.5 million\u7ec6\u80de<\/li>\n\n\n\n
      3. \u5b54\u677f\u7684\u79bb\u5fc3\u7528\u4e864\u2103, 1000 x g, 30min<\/li>\n\n\n\n
      4. \u611f\u67d3\u75c5\u6bd2\u540e3\u5929\u624d\u8dd1FACS\uff08\u4e0d\u77e5\u9053\u660e\u5929\u5bcc\u96c6\u7684\u65f6\u5019\u4f1a\u4e0d\u4f1a\u9633\u6027\u7387\u66f4\u9ad8\uff09<\/li>\n<\/ol>\n\n\n\n
        \"\"
        \u975e\u5e38\u5938\u5f20\u7684\u9633\u6027\u6bd4\u4f8b<\/figcaption><\/figure>\nhttps:\/\/fanyiming.life\/?p=10519<\/a><\/cite><\/blockquote>\n","protected":false},"excerpt":{"rendered":"

        \u8fd9\u7bc7\u7b14\u8bb0\u8be6\u7ec6\u8bb0\u5f55\u4e86K562\u7ec6\u80de\u611f\u67d3\u6162\u75c5\u6bd2\u7684\u5177\u4f53\u5b9e\u9a8c\u6d41\u7a0b\uff0c\u5305\u62ec\u75c5\u6bd2\u5305\u88c5\u3001\u8f6c\u67d3\u6761\u4ef6\u3001\u79bb\u5fc3\u53c2\u6570\u53ca\u611f\u67d3\u540e\u7684\u5173\u952e\u65f6\u95f4\u70b9\u3002\u4f5c\u8005\u7279\u522b\u5f3a\u8c03\u4e86\u75c5\u6bd2\u6536\u83b7\u65f6\u673a\u4e0e\u611f\u67d3\u540e\u57f9\u517b\u65f6\u95f4\u5bf9\u8367\u5149\u9633\u6027\u7387\u7684\u663e\u8457\u5f71\u54cd\uff0c\u5e76\u5206\u4eab\u4e86\u83b7\u5f97\u8d85\u9ad8\u611f\u67d3\u6548\u7387\u7684\u4f18\u5316\u6761\u4ef6\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-9598","post","type-post","status-publish","format-standard","hentry","category-note"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/9598","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9598"}],"version-history":[{"count":4,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/9598\/revisions"}],"predecessor-version":[{"id":13870,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/9598\/revisions\/13870"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9598"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9598"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9598"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}