{"id":9947,"date":"2025-05-01T06:35:33","date_gmt":"2025-04-30T22:35:33","guid":{"rendered":"http:\/\/localhost\/?p=9947"},"modified":"2025-12-21T01:03:49","modified_gmt":"2025-12-20T17:03:49","slug":"%e9%95%bf%e4%b8%8a%e4%b8%8b%e6%96%87vs-rag","status":"publish","type":"post","link":"https:\/\/fanyiming.life\/?p=9947","title":{"rendered":"\u957f\u4e0a\u4e0b\u6587vs.RAG"},"content":{"rendered":"\n<p>\u554a~~\u53c8\u6298\u817e\u4e86\u4e00\u663c\u591c\uff0c\u5b8c\u6210\u4e86\u4e00\u76f4\u60f3\u505a\u7684\u4e8b\u60c5\uff0c\u7ed9\u7f51\u7ad9\u6dfb\u52a0\u4e86AI\u641c\u7d22\u3002\u4f17\u6240\u5468\u77e5\uff0c\u628a\u6574\u4e2a\u7f51\u7ad9\u6240\u6709\u6587\u672c\u4f5c\u4e3aprompt\u8f93\u5165\u7ed9LLM\u662f\u4e0d\u73b0\u5b9e\u7684\uff0c\u867d\u8bf4\u767e\u4e07\u4e0a\u4e0b\u6587\u7684\u6a21\u578b\u4e5f\u51fa\u73b0\u4e86\uff0c\u4f46\u662f\u4e00\u6b21\u68c0\u7d22\u5c31\u8981\u82b1\u51e0\u5757\u94b1\u7684\u6a21\u578b\u8c03\u7528\u8d39\u7528\u5b9e\u5728\u662f\u96be\u4ee5\u627f\u53d7\u3002<\/p>\n\n\n\n<p>\u6240\u4ee5\u5728\u8fd9\u4e2a\u529f\u80fd\u7684\u57fa\u7840\u4e0a\uff0c\u6211\u53c8\u52a0\u4e86\u4e00\u4e2a\u5411\u91cf\u6570\u636e\u5e93\u4f5c\u4e3a\u6587\u7ae0\u5185\u5bb9\u7684\u50a8\u5b58\u548c\u68c0\u7d22\u3002\u5373\u6bcf\u6b21AI\u641c\u7d22\u524d\uff0c\u5148\u901a\u8fc7\u5411\u91cf\u68c0\u7d22\u8fd4\u56de\u82e5\u5e72\u4e2a\u6700\u76f8\u5173\u6bb5\u843d\uff0c\u7136\u540e\u4ee5\u8fd9\u4e9b\u6bb5\u843d\u4f5c\u4e3aprompt\u6295\u7ed9LLM\uff08\u4e0d\u77e5\u9053\u662f\u4e0d\u662f\u80fd\u591f\u78b0\u74f7RAG\u8fd9\u4e2a\u6982\u5ff5\uff09\u3002<\/p>\n\n\n\n<p>\u5f53\u7136\uff0cRAG\u672c\u8d28\u4e0a\u662f\u4e3a\u4e86\u7f29\u77edprompt\u4e0d\u5f97\u5df2\u800c\u4e3a\u4e4b\u7684\u65b9\u6cd5\uff0c\u80af\u5b9a\u4ece\u4fe1\u606f\u5b8c\u6574\u6027\u6765\u8bf4\u5dee\u4e86\u5f88\u591a\u5f88\u591a\u3002\u90a3\u4e48\u7a76\u7adf\u5dee\u8ddd\u6709\u591a\u5927\u5462\uff1f\u8ba9\u6211\u4eec\u6b64\u523b\u5229\u7528\u5c0f\u7ad9\u81ea\u5df1\u7684\u535a\u5ba2\u6570\u636e\u6765\u76f4\u89c2\u611f\u53d7\u4e00\u4e0b\uff1a<\/p>\n\n\n\n<p>\u68c0\u7d22\u8bcd\uff1a\u201c\u535a\u4e3b\u662f\u4e2a\u600e\u6837\u7684\u4eba\uff1f\u201d<\/p>\n\n\n\n<p>\u9996\u5148\u662fRAG\u7ed3\u679c\uff08model: deepseek-chat\uff0c \u6bcf\u4e2a\u5411\u91cf\u68c0\u7d22\u6bb5\u843d\u4e0d\u8d85\u8fc72000\u5b57\u7b26\uff0c\u68c0\u7d22\u5f97\u523020\u4e2a\u6700\u76f8\u5173\u7684\u6bb5\u843d\u6295\u5582\u5927\u6a21\u578b\uff09\uff1a<\/p>\n\n\n\n<div style=\"border: 1px solid black\">\n  <p>\u6839\u636e\u7f51\u7ad9\u5185\u5bb9\uff0c\u535a\u4e3bEamon\u662f\u4e00\u4e2a\u5177\u6709\u591a\u91cd\u7279\u8d28\u7684\u4eba\uff0c\u4ee5\u4e0b\u662f\u4ece\u4e0d\u540c\u89d2\u5ea6\u6574\u7406\u7684\u8be6\u7ec6\u4fe1\u606f\uff1a<\/p>\n  <h3>1. <strong>\u5b66\u672f\u8eab\u4efd\u4e0e\u804c\u4e1a\u72b6\u6001<\/strong><\/h3>\n  <ul>\n    <li><strong>\u751f\u7269\u5b66\u7814\u7a76\u8005<\/strong>\uff1a\u76ee\u524d\u4e13\u6ce8\u4e8e\u6838\u808c\u52a8\u86cb\u767d\uff08Nuclear actins\uff09\u548cRNA\u8fde\u63a5\uff08RNA ligation\uff09\u7814\u7a76\uff0c\u5904\u4e8e\u535a\u58eb\u9636\u6bb5\uff08<a href=\"\/?p=3062\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"3062\" title=\"NowPage\" target=\"_blank\">\u6587\u6863 #3062<\/a>\u3001<a href=\"\/?p=6055\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"1\" title=\"\u4f18\u79c0\u6bd5\u4e1a\u751f\u8bc4\u9009\" target=\"_blank\">\u6bb5\u843d #1<\/a>\uff09\u3002<\/li>\n    <li><strong>\u5b66\u672f\u6001\u5ea6<\/strong>\uff1a\u5bf9\u5b66\u672f\u6c1b\u56f4\u6709\u5f3a\u70c8\u6279\u5224\u6027\uff0c\u66fe\u8868\u793a\u82e5\u9700\u59a5\u534f\u5b66\u672f\u7406\u60f3\u4f1a\u8003\u8651\u9000\u51fa\u884c\u4e1a\uff0c\u5177\u6709\u7406\u60f3\u4e3b\u4e49\u503e\u5411\uff08<a href=\"\/?p=165\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"7\" title=\"\u7528\u4e8eCRISPR\u7684gRNA\u8bbe\u8ba1\" target=\"_blank\">\u6bb5\u843d #7<\/a>\u3001<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"11\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #11<\/a>\uff09\u3002<\/li>\n    <li><strong>\u79d1\u7814\u7ecf\u5386<\/strong>\uff1a\u66fe\u53c2\u4e0eNature\u7ea7\u522b\u7684\u7814\u7a76\u9879\u76ee\uff0c\u4f46\u81ea\u5632\u4e3a&#8221;\u6df7\u5b50&#8221;\uff0c\u4f53\u73b0\u8c26\u900a\u4e0e\u5e7d\u9ed8\uff08<a href=\"\/?p=4719\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"17\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #17<\/a>\uff09\u3002<\/li>\n  <\/ul>\n  <h3>2. <strong>\u6027\u683c\u7279\u70b9<\/strong><\/h3>\n  <ul>\n    <li><strong>\u6267\u7740\u4e0e\u7406\u60f3\u4e3b\u4e49<\/strong>\uff1a\u4e60\u60ef&#8221;\u4e00\u6761\u8def\u8d70\u5230\u9ed1&#8221;\uff0c\u5bf9\u8ba4\u5b9a\u7684\u4e8b\uff08\u5982\u79d1\u7814\u3001\u53c2\u519b\uff09\u6709\u5f3a\u70c8\u575a\u6301\uff0c\u4f46\u4e5f\u53ef\u80fd\u56e0\u9884\u8bbe\u7ed3\u679c\u5ffd\u7565\u77db\u76fe\u6570\u636e\uff08<a href=\"\/?p=165\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"7\" title=\"\u7528\u4e8eCRISPR\u7684gRNA\u8bbe\u8ba1\" target=\"_blank\">\u6bb5\u843d #7<\/a>\u3001<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"18\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #18<\/a>\uff09\u3002<\/li>\n    <li><strong>\u5185\u5411\u4e0e\u793e\u4ea4\u77db\u76fe<\/strong>\uff1a\u81ea\u79f0&#8221;i\u4eba&#8221;\uff0c\u4eab\u53d7\u96c6\u4f53\u4e2d\u7684\u900f\u660e\u611f\uff0c\u4f46\u5e38\u56e0&#8221;\u4e0d\u60f3\u793e\u4ea4&#8221;\u800c\u758f\u79bb\uff08<a href=\"\/?p=6055\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"1\" title=\"\u4f18\u79c0\u6bd5\u4e1a\u751f\u8bc4\u9009\" target=\"_blank\">\u6bb5\u843d #1<\/a>\u3001<a href=\"\/?p=7803\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"4\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #4<\/a>\uff09\u3002\u5bf9\u4eba\u9645\u8fde\u63a5\u6301\u60b2\u89c2\u6001\u5ea6\uff0c\u8ba4\u4e3a\u5173\u7cfb\u591a\u57fa\u4e8e\u4ef7\u503c\u4ea4\u6362\uff08<a href=\"\/?p=5152\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"5\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #5<\/a>\uff09\u3002<\/li>\n    <li><strong>\u81ea\u7701\u4e0e\u6210\u957f<\/strong>\uff1a\u9891\u7e41\u8bb0\u5f55\u601d\u60f3\u53d8\u5316\uff0c\u5982\u4ece&#8221;\u8363\u8a89\u865a\u65e0\u4e3b\u4e49&#8221;\u5230\u63a5\u53d7\u751f\u6d3b&#8221;\u9c7c\u7f38\u7406\u8bba&#8221;\u7684\u8f6c\u53d8\uff08<a href=\"\/?p=8059\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"6\" title=\"\u4e09\u89c2\" target=\"_blank\">\u6bb5\u843d #6<\/a>\u3001<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"13\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #13<\/a>\uff09\u3002<\/li>\n  <\/ul>\n  <h3>3. <strong>\u751f\u6d3b\u4e0e\u5174\u8da3<\/strong><\/h3>\n  <ul>\n    <li><strong>\u9f99\u821f\u961f\u961f\u957f<\/strong>\uff1a\u66fe\u6295\u5165\u5927\u91cf\u65f6\u95f4\u8bad\u7ec3\uff0c\u6ce8\u91cd\u56e2\u961f\u4f20\u627f\uff0c\u88ab\u63cf\u8ff0\u4e3a&#8221;\u81ea\u4fe1\u3001\u81ea\u5f8b\u3001\u4e50\u89c2&#8221;\uff08<a href=\"\/?p=4449\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"9\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #9<\/a>\uff09\u3002<\/li>\n    <li><strong>\u953b\u70bc\u4e60\u60ef<\/strong>\uff1a\u901a\u8fc7\u5355\u6760\u8bad\u7ec3\u89e3\u538b\uff0c\u4ece\u7626\u5f31\u53d8\u5f97\u5f3a\u58ee\uff08<a href=\"\/?p=165\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"7\" title=\"\u7528\u4e8eCRISPR\u7684gRNA\u8bbe\u8ba1\" target=\"_blank\">\u6bb5\u843d #7<\/a>\u3001<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"11\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #11<\/a>\uff09\u3002<\/li>\n    <li><strong>\u6587\u827a\u504f\u597d<\/strong>\uff1a\u559c\u6b22\u7b80\u6d01\u6216\u590d\u6742\u7684\u7f8e\u5b66\uff0c\u6b23\u8d4f\u827a\u672f\u5316\u4fee\u9970\u4f46\u533a\u5206\u73b0\u5b9e\u4e0e\u827a\u672f\uff08<a href=\"\/?p=136\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"3\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #3<\/a>\uff09\u3002<\/li>\n  <\/ul>\n  <h3>4. <strong>\u4ef7\u503c\u89c2\u4e0e\u54f2\u5b66\u601d\u8003<\/strong><\/h3>\n  <ul>\n    <li><strong>\u4eba\u751f\u89c2<\/strong>\uff1a\u8ba4\u4e3a\u4eba\u751f\u65e0\u9884\u8bbe\u76ee\u7684\uff0c\u91cd\u89c6\u4f53\u9a8c\u800c\u975e\u7ed3\u679c\uff0c\u611f\u6069\u73af\u5883\u4e0e\u673a\u9047\uff08<a href=\"\/?p=136\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"3\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #3<\/a>\u3001<a href=\"\/?p=46\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"12\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #12<\/a>\uff09\u3002<\/li>\n    <li><strong>\u793e\u4f1a\u89c2<\/strong>\uff1a\u6279\u5224\u5229\u5df1\u4e3b\u4e49\uff0c\u4e3b\u5f20\u521b\u9020&#8221;\u4e0d\u5f15\u8bf1\u4eba\u6027\u5e7d\u6697\u7684\u73af\u5883&#8221;\uff0c\u540c\u65f6\u627f\u8ba4\u81ea\u8eab\u77db\u76fe\uff08<a href=\"\/?p=7803\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"4\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #4<\/a>\u3001<a href=\"\/?p=153\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"16\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #16<\/a>\uff09\u3002<\/li>\n    <li><strong>\u5b64\u72ec\u611f<\/strong>\uff1a\u6df1\u523b\u4f53\u4f1a\u4eba\u9645\u9694\u9602\uff0c\u8ba4\u4e3a&#8221;\u771f\u5b9e\u81ea\u6211&#8221;\u96be\u88ab\u63a5\u7eb3\uff0c\u9009\u62e9&#8221;\u6210\u5e74\u4eba\u865a\u4f2a\u7684\u4f53\u9762&#8221;\uff08<a href=\"\/?p=5152\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"5\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #5<\/a>\uff09\u3002<\/li>\n  <\/ul>\n  <h3>5. <strong>\u6587\u5b57\u98ce\u683c\u4e0e\u81ea\u6211\u8868\u8fbe<\/strong><\/h3>\n  <ul>\n    <li><strong>\u76f4\u7387\u4e0e\u5e7d\u9ed8<\/strong>\uff1a\u81ea\u79f0&#8221;\u534a\u540a\u5b50&#8221;\uff0c\u535a\u5ba2&#8221;\u4e0d\u6ce8\u91cd\u8bfb\u8005\u4f53\u9a8c\u53ea\u4e3a\u81ea\u5df1\u597d\u73a9&#8221;\uff08\u6bb5\u843d #\uff09\u3002<\/li>\n    <li><strong>\u6df1\u5ea6\u601d\u8003<\/strong>\uff1a\u5e38\u63a2\u8ba8\u4e09\u89c2\u3001\u5b64\u72ec\u3001\u5b66\u672f\u4f26\u7406\u7b49\u8bdd\u9898\uff0c\u6587\u98ce\u4ecb\u4e8e\u7406\u6027\u5206\u6790\u4e0e\u611f\u6027\u503e\u8bc9\u4e4b\u95f4\uff08<a href=\"\/?p=136\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"3\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #3<\/a>\u3001<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"13\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #13<\/a>\uff09\u3002<\/li>\n  <\/ul>\n  <h3>\u603b\u7ed3<\/h3>\n  <p>Eamon\u662f\u4e00\u4e2a\u590d\u6742\u800c\u7acb\u4f53\u7684\u5f62\u8c61\uff1a\u65e2\u662f\u6267\u7740\u4e8e\u79d1\u7814\u7406\u60f3\u7684\u535a\u58eb\u751f\uff0c\u53c8\u662f\u5bf9\u4eba\u6027\u4e0e\u793e\u4f1a\u4fdd\u6301\u51b7\u5cfb\u89c2\u5bdf\u7684\u601d\u8003\u8005\uff1b\u65e2\u6709\u5185\u5411\u758f\u79bb\u7684\u4e00\u9762\uff0c\u53c8\u6709\u9f99\u821f\u961f\u4e2d\u7684\u9886\u5bfc\u529b\u3002\u4ed6\u7684\u535a\u5ba2\u8bb0\u5f55\u4e86\u5927\u91cf\u81ea\u6211\u5256\u6790\u4e0e\u77db\u76fe\uff0c\u5c55\u73b0\u4e86\u4e00\u4e2a\u5728\u5b66\u672f\u3001\u793e\u4ea4\u4e0e\u4ef7\u503c\u89c2\u63a2\u7d22\u4e2d\u4e0d\u65ad\u6210\u957f\u7684\u5e74\u8f7b\u4eba\u5f62\u8c61\u3002<\/p>\n  <p>\uff08\u5f15\u7528\u6765\u6e90\uff1a\u6bb5\u843d #\u3001#1\u3001#3\u3001#4\u3001#5\u3001#6\u3001#7\u3001#9\u3001#11\u3001#12\u3001#13\u3001#16\u3001#17\u3001#18\uff09<\/p>\n<\/div>\n\n\n\n<p>\u7136\u540e\u662f\u5168\u6587\u4f5c\u4e3aprompt\uff08model: deepseek-chat\uff09\uff1a<\/p>\n\n\n\n<div style=\"border: 1px solid black\"><div class=\"ai-search-error\" style=\"color: #dc3545;padding: 10px;margin: 5px 0;background: #fff0f0;border-radius: 4px;border-left: 4px solid #dc3545\">API request failed with status 400: This model&#8217;s maximum context length is 65536 tokens. However, you requested 228124 tokens (227124 in the messages, 1000 in the completion). Please reduce the length of the messages or completion.<\/div><\/div>\n\n\n\n<p>\u54c8\u54c8\uff0c\u4e0d\u51fa\u610f\u6599\u7684\uff0c\u5168\u7ad9\u5185\u5bb9\u670920\u591a\u4e07tokens\uff0cdeepseek\u6700\u957f\u8f93\u51656.5\u4e07\uff0c\u7206\u4e86\u2026\u2026<\/p>\n\n\n\n<p>\u6362\u6210\u963f\u91cc\u7684Qwen-long\uff08\u53f7\u79f01000\u4e07\u4e0a\u4e0b\u6587\u957f\u5ea6\uff09\u8bd5\u8bd5\u3002\u516c\u5e73\u8d77\u89c1\uff0c\u4e5f\u5148\u8dd1\u4e2a\u5411\u91cf\u68c0\u7d22\uff1a<\/p>\n\n\n\n<div style=\"border: 1px solid black\">\n  <p>\u4ece\u63d0\u4f9b\u7684\u6bb5\u843d\u4e2d\uff0c\u6211\u4eec\u53ef\u4ee5\u603b\u7ed3\u51fa\u535a\u4e3bEamon\u7684\u4e00\u4e9b\u7279\u5f81\u548c\u89c2\u70b9\uff1a<\/p>\n  <ol>\n    <li>\n      <p><strong>\u4e2a\u4eba\u80cc\u666f<\/strong>\uff1aEamon\u662f\u4e00\u4e2a\u5b66\u4e60\u751f\u7269\u7684\u5b66\u751f\uff08<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"11\" title=\"\u5173\u4e8e\" target=\"_blank\">\u6587\u6863 #11<\/a>\uff0c<a href=\"\/?p=233\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"0\" title=\"\u5173\u4e8e\" target=\"_blank\">\u6bb5\u843d #0<\/a>\uff09\u3002\u4ed6\u76ee\u524d\u7684\u72b6\u6001\u662f\u7814\u7a76\u6838\u808c\u52a8\u86cb\u767d\u548cRNA\u8fde\u63a5\uff08Studying Nuclear actins &amp; RNA ligation\uff09\uff08<a href=\"\/?p=3062\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"3062\" title=\"NowPage\" target=\"_blank\">\u6587\u6863 #3062<\/a>\uff0c<a href=\"\/?p=6055\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"1\" title=\"\u4f18\u79c0\u6bd5\u4e1a\u751f\u8bc4\u9009\" target=\"_blank\">\u6bb5\u843d #1<\/a>\uff09\u3002<\/p>\n    <\/li>\n    <li>\n      <p><strong>\u6027\u683c\u7279\u70b9<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u8ba4\u4e3a\u81ea\u5df1\u662f\u4e00\u4e2a\u6709\u6267\u5ff5\u7684\u4eba\uff0c\u6bd4\u5982\u53c2\u519b\u7684\u6267\u5ff5\u548c\u73b0\u5728\u79d1\u7814\u7684\u6267\u5ff5\u3002\u4ed6\u53ef\u80fd\u4f1a\u610f\u8bc6\u5230\u9009\u62e9\u53ef\u80fd\u5b58\u5728\u95ee\u9898\uff0c\u4f46\u4ecd\u7136\u4f1a\u575a\u6301\u5230\u5e95\uff0c\u76f4\u5230\u611f\u5230\u65e0\u529b\u624d\u4f1a\u653e\u5f03\uff08<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"65\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6587\u6863 #65<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"10\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #10<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u5bf9\u8eab\u8fb9\u4e8b\u60c5\u7684\u5305\u5bb9\u5ea6\u5f88\u5927\uff0c\u5e76\u4e14\u5728\u6e34\u671b\u63a2\u6c42\u4e16\u754c\u8fd0\u884c\u672c\u6e90\u7684\u540c\u65f6\uff0c\u80fd\u591f\u4e0e\u5468\u56f4\u4eba\u5efa\u7acb\u8054\u7ed3\uff0c\u91ca\u653e\u5584\u610f\uff08<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"139\" title=\"\u5f00\u609f\" target=\"_blank\">\u6587\u6863 #139<\/a>\uff0c<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"11\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #11<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u751f\u6d3b\u6001\u5ea6<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u5bf9\u751f\u6d3b\u7684\u6001\u5ea6\u8f83\u4e3a\u4f5b\u7cfb\uff0c\u8ba4\u4e3a\u4eba\u751f\u76ee\u6807\u53ef\u4ee5\u5f88\u968f\u610f\uff0c\u5f53\u524d\u7684\u76ee\u6807\u662f\u52aa\u529b\u6210\u4e3a\u4e00\u4e2a\u5408\u683c\u7684\u751f\u7269\u5b66\u7814\u7a76\u5b66\u8005\uff0c\u4f46\u8fd9\u66f4\u50cf\u662f\u4e3a\u4e86\u627e\u70b9\u4e8b\u505a\uff0c\u505a\u4e0d\u5230\u4e5f\u65e0\u6240\u8c13\uff08<a href=\"\/?p=136\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"136\" title=\"\u4e09\u89c2\" target=\"_blank\">\u6587\u6863 #136<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"2\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6bb5\u843d #2<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u5f3a\u8c03\u4f53\u9a8c\u7684\u91cd\u8981\u6027\uff0c\u8ba4\u4e3a\u4eba\u751f\u91cd\u5728\u4f53\u9a8c\uff0c\u76ee\u6807\u53ea\u662f\u83b7\u5f97\u66f4\u597d\u4f53\u9a8c\u7684\u624b\u6bb5\u3002\u5982\u679c\u76ee\u6807\u8ba9\u751f\u6d3b\u4f53\u9a8c\u53d8\u5dee\uff0c\u5b81\u613f\u820d\u5f03\u76ee\u6807\uff08<a href=\"\/?p=46\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"46\" title=\"\u81ea\u8bf4\u81ea\u8bdd1\" target=\"_blank\">\u6587\u6863 #46<\/a>\uff0c<a href=\"\/?p=46\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"13\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #13<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u4ef7\u503c\u89c2<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u8ba4\u4e3a\u4eba\u751f\u81ea\u7531\uff0c\u4e0d\u5b58\u5728\u9884\u8bbe\u7684\u4eba\u751f\u76ee\u7684\uff0c\u4f46\u4ed6\u9700\u8981\u8bbe\u5b9a\u4e00\u4e2a\u5b58\u5728\u611f\u3002\u4ed6\u8ba4\u4e3a\u4e0d\u5e94\u6d6a\u8d39\u65f6\u95f4\u5728\u865a\u8363\u3001\u9893\u4e27\u3001\u91d1\u94b1\u548c\u5916\u4eba\u7684\u8bc4\u4ef7\u7b49\u65e0\u5173\u7d27\u8981\u7684\u4e8b\u60c5\u4e0a\uff08<a href=\"\/?p=136\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"136\" title=\"\u4e09\u89c2\" target=\"_blank\">\u6587\u6863 #136<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"2\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6bb5\u843d #2<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u53cd\u5bf9\u5c06\u9e21\u6bdb\u849c\u76ae\u548c\u4eba\u6027\u9634\u6697\u9762\u6beb\u65e0\u906e\u63a9\u5730\u5c55\u793a\uff0c\u559c\u6b22\u7ecf\u8fc7\u827a\u672f\u5316\u4fee\u9970\u7684\u4e8b\u7269\uff08<a href=\"\/?p=136\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"136\" title=\"\u4e09\u89c2\" target=\"_blank\">\u6587\u6863 #136<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"2\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6bb5\u843d #2<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u793e\u4ea4\u89c2<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u9010\u6e10\u8fdc\u79bb\u4e86\u793e\u4ea4\u5e73\u53f0\uff0c\u4e0d\u518d\u613f\u610f\u5206\u4eab\u81ea\u5df1\u7684\u751f\u6d3b\uff0c\u4e5f\u4e0d\u771f\u6b63\u5173\u5fc3\u670b\u53cb\u7684\u751f\u6d3b\u7ec6\u8282\uff08<a href=\"\/?p=5152\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"5152\" title=\"\u865a\u4f2a\" target=\"_blank\">\u6587\u6863 #5152<\/a>\uff0c<a href=\"\/?p=165\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"7\" title=\"\u7528\u4e8eCRISPR\u7684gRNA\u8bbe\u8ba1\" target=\"_blank\">\u6bb5\u843d #7<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u8ba4\u8bc6\u5230\u4eba\u4e0e\u4eba\u4e4b\u95f4\u7684\u8fde\u63a5\u5f88\u591a\u65f6\u5019\u4ec5\u9650\u4e8e\u4ef7\u503c\u4ea4\u6362\uff0c\u60c5\u611f\u4e5f\u662f\u4e3a\u4e86\u66f4\u65b9\u4fbf\u5730\u4ea4\u6362\uff08<a href=\"\/?p=5152\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"5152\" title=\"\u865a\u4f2a\" target=\"_blank\">\u6587\u6863 #5152<\/a>\uff0c<a href=\"\/?p=165\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"7\" title=\"\u7528\u4e8eCRISPR\u7684gRNA\u8bbe\u8ba1\" target=\"_blank\">\u6bb5\u843d #7<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u4e16\u754c\u89c2<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u5bf9\u8363\u8a89\u548c\u8bc4\u5956\u6301\u6279\u5224\u6001\u5ea6\uff0c\u8ba4\u4e3a\u8fd9\u4e9b\u5956\u9879\u662f\u7531\u89c4\u5219\u5236\u5b9a\u8005\u4eba\u4e3a\u8bbe\u7f6e\u7684\uff0c\u76ee\u7684\u662f\u4f20\u8fbe\u4e0a\u5c42\u7684\u884c\u4e3a\u89c4\u8303\u548c\u4ef7\u503c\u89c2\u5f15\u5bfc\uff08<a href=\"\/?p=8059\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"8059\" title=\"\u9c7c\u7f38\" target=\"_blank\">\u6587\u6863 #8059<\/a>\uff0c<a href=\"\/?p=8059\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"5\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #5<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u610f\u8bc6\u5230\u4e2d\u6587\u4e16\u754c\u5904\u4e8e\u73b0\u4ee3\u6587\u660e\u7684\u8fb9\u7f18\u5730\u5e26\uff0c\u4fe1\u606f\u6765\u6e90\u6709\u9650\uff08<a href=\"\/?p=4605\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"4605\" title=\"\u73b0\u4ee3\u6587\u660e\u8fb9\u7f18\u5730\u5e26\" target=\"_blank\">\u6587\u6863 #4605<\/a>\uff0c<a href=\"\/?p=4605\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"6\" title=\"\u4e09\u89c2\" target=\"_blank\">\u6bb5\u843d #6<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u4e09\u89c2\u6574\u7406<\/strong>\uff1a<\/p>\n      <ul>\n        <li>\u5728\u6574\u7406\u4e09\u89c2\u7684\u8fc7\u7a0b\u4e2d\uff0cEamon\u610f\u8bc6\u5230\u4e86\u5f88\u591a\u5e95\u5c42\u89c2\u5ff5\u7684\u4e0d\u6e05\u6670\uff0c\u4f8b\u5982\u4eba\u751f\u610f\u4e49\u3001\u7edd\u5bf9\u771f\u7406\u7684\u5b58\u5728\u6027\u3001\u79d1\u5b66\u4e0e\u54f2\u5b66\u7684\u5173\u7cfb\u7b49\uff08<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"139\" title=\"\u5f00\u609f\" target=\"_blank\">\u6587\u6863 #139<\/a>\uff0c<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"11\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #11<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u5bf9\u4e16\u754c\u7684\u770b\u6cd5\u53d8\u5f97\u66f4\u52a0\u5e73\u548c\uff0c\u4e0d\u518d\u6709\u201c\u77a7\u4e0d\u8d77\u8c01\u201d\u7684\u5fc3\u601d\uff0c\u5bf9\u81ea\u5df1\u7684\u5305\u5bb9\u5ea6\u4e5f\u589e\u52a0\u4e86\uff08<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"139\" title=\"\u5f00\u609f\" target=\"_blank\">\u6587\u6863 #139<\/a>\uff0c<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"11\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #11<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u672a\u6765\u5c55\u671b<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u8bbe\u60f3\u4e94\u5e74\u540e\u6216\u5341\u5e74\u540e\u7684\u751f\u6d3b\u573a\u666f\u662f\u5728\u4e00\u4e2a\u201c\u4e0e\u4e16\u9694\u7edd\u201d\u7684\u7814\u7a76\u673a\u6784\u91cc\u6bcf\u5929\u5e73\u6de1\u5730\u505a\u7740\u81ea\u5df1\u7684\u7814\u7a76\uff0c\u6574\u7406\u548c\u5b66\u4e60\uff08<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"65\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6587\u6863 #65<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"10\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #10<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u5bf9\u5b66\u672f\u6c14\u6c1b\u4e0d\u6ee1\u610f\uff0c\u8868\u793a\u5982\u679c\u8feb\u4e8e\u73b0\u5b9e\u538b\u529b\u8981\u505a\u51fa\u8ba9\u672c\u79d1\u65f6\u7684\u81ea\u5df1\u8ba8\u538c\u7684\u884c\u4e3a\uff0c\u4f1a\u9009\u62e9\u9000\u51fa\u884c\u4e1a\u6765\u4fdd\u5168\u5fc3\u4e2d\u7684\u5723\u5730\uff08<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"65\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6587\u6863 #65<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"10\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #10<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n  <\/ol>\n  <p>\u7efc\u4e0a\u6240\u8ff0\uff0cEamon\u662f\u4e00\u4e2a\u5bf9\u751f\u6d3b\u6709\u7740\u6df1\u523b\u601d\u8003\u7684\u4eba\uff0c\u4ed6\u5728\u8ffd\u6c42\u79d1\u5b66\u7814\u7a76\u7684\u540c\u65f6\uff0c\u4e5f\u5728\u4e0d\u65ad\u63a2\u7d22\u81ea\u6211\u548c\u4e16\u754c\u7684\u672c\u8d28\u3002\u4ed6\u5bf9\u5f85\u751f\u6d3b\u548c\u5de5\u4f5c\u7684\u6001\u5ea6\u8f83\u4e3a\u4f5b\u7cfb\uff0c\u6ce8\u91cd\u4f53\u9a8c\u800c\u975e\u76ee\u6807\uff0c\u540c\u65f6\u5bf9\u793e\u4f1a\u73b0\u8c61\u6709\u7740\u72ec\u7279\u7684\u89c1\u89e3\u548c\u6279\u5224\u7cbe\u795e\u3002<\/p>\n<\/div>\n\n\n\n<p>\u7136\u540e\u8bd5\u8bd5\u5168\u7ad9\u5168\u6587\u68c0\u7d22\uff1a<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"82\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/05\/20250501021402216-1024x82.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-9964\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/05\/20250501021402216-1024x82.png?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/05\/20250501021402216-300x24.png?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/05\/20250501021402216-768x61.png?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/05\/20250501021402216.png?x-oss-process=style\/default 1313w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>\u6211\u76f4\u63a5\u4e00\u4e2a\u554a\uff1f\u6211\u66fe\u7ecf\u5199\u8fc7\u4ec0\u4e48\u6c61\u79fd\u4e4b\u8bed\u5417\uff1f\uff1f\u66f4\u79bb\u8c31\u7684\u662f\uff0c\u6211\u5207\u56de\u5411\u91cf\u68c0\u7d22\uff0c\u60f3\u76f4\u63a5\u641c\u4e00\u4e0b\u6709\u4ec0\u4e48\u654f\u611f\u5185\u5bb9\uff0c\u7ed3\u679c\u8fd9\u6b21\u662foutput\u51fa\u73b0\u4e86\u654f\u611f\u5185\u5bb9\u2026\u2026<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"71\" src=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/05\/20250501023126486-1024x71.png?x-oss-process=style\/default\" alt=\"\" class=\"wp-image-9966\" srcset=\"https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/05\/20250501023126486-1024x71.png?x-oss-process=style\/default 1024w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/05\/20250501023126486-300x21.png?x-oss-process=style\/default 300w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/05\/20250501023126486-768x53.png?x-oss-process=style\/default 768w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/05\/20250501023126486-1536x106.png?x-oss-process=style\/default 1536w, https:\/\/fanym.oss-cn-beijing.aliyuncs.com\/wp-content\/uploads\/2025\/05\/20250501023126486.png?x-oss-process=style\/default 1592w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>\u4e4b\u540e\u53c8\u8bd5\u4e86\u4e00\u6b21\u53ef\u4ee5\u4e86\uff0c\u4e5f\u4e0d\u77e5\u9053\u963f\u91cc\u4e91\u90a3\u4e2a\u5ba1\u67e5\u662f\u600e\u4e48\u641e\u7684\uff1a<\/p>\n\n\n\n<div><p>\u6839\u636e\u63d0\u4f9b\u7684\u6587\u6863\u5185\u5bb9\uff0c\u5173\u4e8e\u201c\u535a\u4e3b\u662f\u600e\u6837\u7684\u4eba\uff1f\u201d\u53ef\u4ee5\u4ece\u4ee5\u4e0b\u51e0\u4e2a\u8981\u70b9\u8fdb\u884c\u7cbe\u786e\u3001\u6df1\u5165\u7684\u89e3\u7b54\uff1a<\/p>\n<h3>1. <strong>\u5174\u8da3\u5e7f\u6cdb\u4e14\u70ed\u7231\u5b66\u4e60<\/strong><\/h3>\n<ul>\n<li>\u535a\u4e3b\u5bf9\u591a\u4e2a\u9886\u57df\u90fd\u6709\u6d53\u539a\u7684\u5174\u8da3\uff0c\u5305\u62ec\u4f46\u4e0d\u9650\u4e8e\u751f\u7269\u79d1\u5b66\u3001\u6444\u5f71\u3001\u7f16\u7a0b\u3001\u54f2\u5b66\u548c\u6587\u5b66\u7b49\u3002\u4f8b\u5982\uff0c\u5728<span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #9883<\/span> \u4e2d\u63d0\u5230\u535a\u4e3b\u4f7f\u7528AI\u89e3\u51b3Wordpress\u8fc1\u79fb\u95ee\u9898\uff0c\u5c55\u793a\u5176\u5bf9\u6280\u672f\u7684\u5174\u8da3\uff1b\u800c\u5728<span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #958<\/span> \u4e2d\uff0c\u535a\u4e3b\u5206\u4eab\u4e86\u81ea\u5df1\u5b66\u4e60\u8fbe\u82ac\u5947\u8f6f\u4ef6\u7684\u7ecf\u5386\uff0c\u8868\u660e\u4ed6\u5bf9\u89c6\u9891\u526a\u8f91\u4e5f\u6709\u6d89\u730e\u3002<\/li>\n<li>\u6b64\u5916\uff0c<span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #467<\/span> \u63d0\u5230\u535a\u4e3b\u901a\u8fc7\u901a\u5bb5\u5b66\u4e60\u5feb\u901f\u638c\u63e1\u8fbe\u82ac\u5947\u526a\u8f91\u6280\u80fd\uff0c\u4f53\u73b0\u4e86\u4ed6\u613f\u610f\u6295\u5165\u65f6\u95f4\u548c\u7cbe\u529b\u53bb\u5b66\u4e60\u65b0\u4e8b\u7269\u7684\u6001\u5ea6\u3002<\/li>\n<\/ul>\n<h3>2. <strong>\u53cd\u601d\u6df1\u523b\u4e14\u6ce8\u91cd\u81ea\u6211\u63d0\u5347<\/strong><\/h3>\n<ul>\n<li>\u535a\u4e3b\u7ecf\u5e38\u5bf9\u81ea\u5df1\u7ecf\u5386\u7684\u4e8b\u60c5\u8fdb\u884c\u6df1\u5ea6\u53cd\u601d\uff0c\u5e76\u4ece\u4e2d\u603b\u7ed3\u7ecf\u9a8c\u6559\u8bad\u3002\u5982\u5728<span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #9883<\/span> \u4e2d\uff0c\u535a\u4e3b\u8be6\u7ec6\u8bb0\u5f55\u4e86wordpress\u8fc1\u79fb\u8fc7\u7a0b\u4e2d\u9047\u5230\u7684\u95ee\u9898\u53ca\u89e3\u51b3\u65b9\u6848\uff0c\u5c55\u73b0\u4e86\u5176\u89e3\u51b3\u95ee\u9898\u7684\u80fd\u529b\u4e0e\u8010\u5fc3\u3002<\/li>\n<li><span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #105<\/span> \u8fdb\u4e00\u6b65\u8bf4\u660e\u4e86\u535a\u4e3b\u5bf9\u6731\u51b0\u8001\u5e08\u7684\u656c\u4f69\u4e4b\u60c5\uff0c\u540c\u65f6\u8868\u8fbe\u4e86\u5e0c\u671b\u81ea\u5df1\u80fd\u591f\u8fbe\u5230\u66f4\u9ad8\u5b66\u672f\u6c34\u5e73\u7684\u613f\u671b\uff0c\u8fd9\u53cd\u6620\u4e86\u535a\u4e3b\u8ffd\u6c42\u5353\u8d8a\u7684\u7cbe\u795e\u3002<\/li>\n<li>\u5728<span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #80<\/span> \u4e2d\uff0c\u535a\u4e3b\u5766\u8a00\u4e0d\u60f3\u518d\u4ece\u4e8b\u57fa\u7840\u7814\u7a76\uff0c\u800c\u662f\u5e0c\u671b\u521b\u9020\u5177\u6709\u5b9e\u9645\u5e94\u7528\u4ef7\u503c\u7684\u6210\u679c\uff0c\u663e\u793a\u51fa\u5176\u601d\u8003\u65b9\u5411\u4ece\u7406\u8bba\u8f6c\u5411\u5b9e\u8df5\u7684\u8fc7\u7a0b\u3002<\/li>\n<\/ul>\n<h3>3. <strong>\u5bcc\u6709\u793e\u4f1a\u8d23\u4efb\u611f\u548c\u4eba\u6587\u5173\u6000<\/strong><\/h3>\n<ul>\n<li>\u535a\u4e3b\u4e0d\u4ec5\u5173\u6ce8\u4e2a\u4eba\u6210\u957f\uff0c\u8fd8\u5173\u5fc3\u793e\u4f1a\u95ee\u9898\u3002\u4f8b\u5982\uff0c\u5728<span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #114<\/span> \u4e2d\uff0c\u5c3d\u7ba1\u5927\u591a\u6570\u4eba\u5bf9\u5b89\u500d\u664b\u4e09\u9047\u523a\u4e8b\u4ef6\u6301\u8c03\u4f83\u6001\u5ea6\uff0c\u4f46\u535a\u4e3b\u5374\u9009\u62e9\u7ad9\u5728\u4eba\u6027\u89d2\u5ea6\u8868\u8fbe\u540c\u60c5\uff0c\u4f53\u73b0\u4e86\u5176\u8f83\u9ad8\u7684\u9053\u5fb7\u610f\u8bc6\u548c\u793e\u4f1a\u8d23\u4efb\u611f\u3002<\/li>\n<li>\u53e6\u5916\uff0c\u4ece<span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #101<\/span> \u53ef\u4ee5\u770b\u51fa\uff0c\u535a\u4e3b\u5bf9\u4e8e\u9886\u5bfc\u529b\u7684\u7406\u89e3\u8d85\u8d8a\u4e86\u7b80\u5355\u7684\u547d\u4ee4\u6267\u884c\u5c42\u9762\uff0c\u5f3a\u8c03\u4ee5\u8eab\u4f5c\u5219\u7684\u91cd\u8981\u6027\uff0c\u8fd9\u79cd\u89c2\u70b9\u900f\u9732\u51fa\u535a\u4e3b\u5e0c\u671b\u6210\u4e3a\u80fd\u4e3a\u4ed6\u4eba\u6811\u7acb\u699c\u6837\u7684\u4eba\u3002<\/li>\n<\/ul>\n<p>\u7efc\u4e0a\u6240\u8ff0\uff0cEamon\u662f\u4e00\u4e2a\u5174\u8da3\u5e7f\u6cdb\u3001\u70ed\u7231\u5b66\u4e60\u3001\u5584\u4e8e\u53cd\u601d\u5e76\u4e0d\u65ad\u8ffd\u6c42\u8fdb\u6b65\u7684\u4eba\uff0c\u540c\u65f6\u4e5f\u5c55\u73b0\u51fa\u5f3a\u70c8\u7684\u793e\u4f1a\u8d23\u4efb\u611f\u548c\u4eba\u6587\u5173\u6000\u3002\u8fd9\u4e9b\u7279\u8d28\u5171\u540c\u5851\u9020\u4e86\u4e00\u4e2a\u7acb\u4f53\u800c\u771f\u5b9e\u7684\u535a\u4e3b\u5f62\u8c61\u3002<\/p>\n<p>\u4ee5\u4e0a\u4fe1\u606f\u5206\u522b\u6765\u6e90\u4e8e\u4ee5\u4e0b\u6587\u6863\uff1a<\/p>\n<ul>\n<li><span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #9883<\/span>\uff08&#8217;\u968f\u7b14&#8217;\u5206\u7c7b\uff0c\u4f5c\u8005&#8217;Eamon&#8217;\uff0c2025-04-24\uff09<\/li>\n<li><span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #467<\/span>\uff08&#8217;\u6444\u5f71, \u7b14\u8bb0&#8217;\u5206\u7c7b\uff0c\u4f5c\u8005&#8217;Eamon&#8217;\uff0c2024-05-23\uff09<\/li>\n<li><span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #105<\/span>\uff08&#8217;\u672a\u5206\u7c7b, \u968f\u7b14&#8217;\u5206\u7c7b\uff0c\u4f5c\u8005&#8217;Eamon&#8217;\uff0c2021-07-21\uff09<\/li>\n<li><span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #80<\/span>\uff08&#8217;\u968f\u7b14&#8217;\u5206\u7c7b\uff0c\u4f5c\u8005&#8217;Eamon&#8217;\uff0c2021-07-21\uff09<\/li>\n<li><span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #114<\/span>\uff08&#8217;\u672a\u5206\u7c7b, \u968f\u7b14&#8217;\u5206\u7c7b\uff0c\u4f5c\u8005&#8217;Eamon&#8217;\uff0c2022-07-10\uff09<\/li>\n<li><span class=\"ai-search-reference-missing\" title=\"\u65e0\u6cd5\u627e\u5230\u6765\u6e90\u94fe\u63a5\">\u6587\u6863 #101<\/span>\uff08&#8217;\u672a\u5206\u7c7b, \u968f\u7b14&#8217;\u5206\u7c7b\uff0c\u4f5c\u8005&#8217;Eamon&#8217;\uff0c2020-02-08\uff09<\/li>\n<\/ul>\n<\/div>\n\n\n\n<p>\u7ed3\u679c\u662f\u8ddf\u9884\u671f\u6070\u6070\u76f8\u53cd\u554a\u3002\u6211\u9884\u8ba1\u662f\u5168\u6587\u6295\u5582\u8f93\u51fa\u5185\u5bb9\u8981\u6bd4RAG\u7cbe\u7ec6\u5f97\u591a\u5462\uff0c\u7ed3\u679c\u5e76\u4e0d\u662f\uff0c\u592a\u957f\u7684\u4e0a\u4e0b\u6587\u8fd8\u4e0d\u5982\u53ea\u7ed9\u7cbe\u534e\u3002<\/p>\n\n\n\n<p>\u4e3a\u4e86\u6392\u9664\u6a21\u578b\u7684\u5f71\u54cd\uff0c\u53c8\u6362\u6210\u667a\u8c31\u6e05\u8a00GLM-4-Long\uff0c\u636e\u8bf4\u4e5f\u662f\u767e\u4e07\u4e0a\u4e0b\u6587\uff0c\u8fd8\u662f\u5148RAG\u4e00\u4e0b\uff1a<\/p>\n\n\n\n<div style=\"border: 1px solid black\">\n  <p>\u4ece\u63d0\u4f9b\u7684\u6bb5\u843d\u4e2d\uff0c\u6211\u4eec\u53ef\u4ee5\u603b\u7ed3\u51fa\u535a\u4e3bEamon\u7684\u4e00\u4e9b\u7279\u5f81\u548c\u89c2\u70b9\uff1a<\/p>\n  <ol>\n    <li>\n      <p><strong>\u4e2a\u4eba\u80cc\u666f<\/strong>\uff1aEamon\u662f\u4e00\u4e2a\u5b66\u4e60\u751f\u7269\u7684\u5b66\u751f\uff08<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"11\" title=\"\u5173\u4e8e\" target=\"_blank\">\u6587\u6863 #11<\/a>\uff0c<a href=\"\/?p=233\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"0\" title=\"\u5173\u4e8e\" target=\"_blank\">\u6bb5\u843d #0<\/a>\uff09\u3002\u4ed6\u76ee\u524d\u7684\u72b6\u6001\u662f\u7814\u7a76\u6838\u808c\u52a8\u86cb\u767d\u548cRNA\u8fde\u63a5\uff08Studying Nuclear actins &amp; RNA ligation\uff09\uff08<a href=\"\/?p=3062\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"3062\" title=\"NowPage\" target=\"_blank\">\u6587\u6863 #3062<\/a>\uff0c<a href=\"\/?p=6055\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"1\" title=\"\u4f18\u79c0\u6bd5\u4e1a\u751f\u8bc4\u9009\" target=\"_blank\">\u6bb5\u843d #1<\/a>\uff09\u3002<\/p>\n    <\/li>\n    <li>\n      <p><strong>\u6027\u683c\u7279\u70b9<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u8ba4\u4e3a\u81ea\u5df1\u662f\u4e00\u4e2a\u6709\u6267\u5ff5\u7684\u4eba\uff0c\u6bd4\u5982\u53c2\u519b\u7684\u6267\u5ff5\u548c\u73b0\u5728\u79d1\u7814\u7684\u6267\u5ff5\u3002\u4ed6\u53ef\u80fd\u4f1a\u610f\u8bc6\u5230\u9009\u62e9\u53ef\u80fd\u5b58\u5728\u95ee\u9898\uff0c\u4f46\u4ecd\u7136\u4f1a\u575a\u6301\u5230\u5e95\uff0c\u76f4\u5230\u611f\u5230\u65e0\u529b\u624d\u4f1a\u653e\u5f03\uff08<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"65\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6587\u6863 #65<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"10\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #10<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u5bf9\u8eab\u8fb9\u4e8b\u60c5\u7684\u5305\u5bb9\u5ea6\u5f88\u5927\uff0c\u5e76\u4e14\u5728\u6e34\u671b\u63a2\u6c42\u4e16\u754c\u8fd0\u884c\u672c\u6e90\u7684\u540c\u65f6\uff0c\u80fd\u591f\u4e0e\u5468\u56f4\u4eba\u5efa\u7acb\u8054\u7ed3\uff0c\u91ca\u653e\u5584\u610f\uff08<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"139\" title=\"\u5f00\u609f\" target=\"_blank\">\u6587\u6863 #139<\/a>\uff0c<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"11\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #11<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u751f\u6d3b\u6001\u5ea6<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u5bf9\u751f\u6d3b\u7684\u6001\u5ea6\u8f83\u4e3a\u4f5b\u7cfb\uff0c\u8ba4\u4e3a\u4eba\u751f\u76ee\u6807\u53ef\u4ee5\u5f88\u968f\u610f\uff0c\u5f53\u524d\u7684\u76ee\u6807\u662f\u52aa\u529b\u6210\u4e3a\u4e00\u4e2a\u5408\u683c\u7684\u751f\u7269\u5b66\u7814\u7a76\u5b66\u8005\uff0c\u4f46\u8fd9\u66f4\u50cf\u662f\u4e3a\u4e86\u627e\u70b9\u4e8b\u505a\uff0c\u505a\u4e0d\u5230\u4e5f\u65e0\u6240\u8c13\uff08<a href=\"\/?p=136\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"136\" title=\"\u4e09\u89c2\" target=\"_blank\">\u6587\u6863 #136<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"2\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6bb5\u843d #2<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u5f3a\u8c03\u4f53\u9a8c\u7684\u91cd\u8981\u6027\uff0c\u8ba4\u4e3a\u4eba\u751f\u91cd\u5728\u4f53\u9a8c\uff0c\u76ee\u6807\u53ea\u662f\u83b7\u5f97\u66f4\u597d\u4f53\u9a8c\u7684\u624b\u6bb5\u3002\u5982\u679c\u76ee\u6807\u8ba9\u751f\u6d3b\u4f53\u9a8c\u53d8\u5dee\uff0c\u5b81\u613f\u820d\u5f03\u76ee\u6807\uff08<a href=\"\/?p=46\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"46\" title=\"\u81ea\u8bf4\u81ea\u8bdd1\" target=\"_blank\">\u6587\u6863 #46<\/a>\uff0c<a href=\"\/?p=46\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"13\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #13<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u4ef7\u503c\u89c2<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u8ba4\u4e3a\u4eba\u751f\u81ea\u7531\uff0c\u4e0d\u5b58\u5728\u9884\u8bbe\u7684\u4eba\u751f\u76ee\u7684\uff0c\u4f46\u4ed6\u9700\u8981\u8bbe\u5b9a\u4e00\u4e2a\u5b58\u5728\u611f\u3002\u4ed6\u8ba4\u4e3a\u4e0d\u5e94\u6d6a\u8d39\u65f6\u95f4\u5728\u865a\u8363\u3001\u9893\u4e27\u3001\u91d1\u94b1\u548c\u5916\u4eba\u7684\u8bc4\u4ef7\u7b49\u65e0\u5173\u7d27\u8981\u7684\u4e8b\u60c5\u4e0a\uff08<a href=\"\/?p=136\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"136\" title=\"\u4e09\u89c2\" target=\"_blank\">\u6587\u6863 #136<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"2\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6bb5\u843d #2<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u53cd\u5bf9\u5c06\u9e21\u6bdb\u849c\u76ae\u548c\u4eba\u6027\u9634\u6697\u9762\u6beb\u65e0\u906e\u63a9\u5730\u5c55\u793a\uff0c\u559c\u6b22\u7ecf\u8fc7\u827a\u672f\u5316\u4fee\u9970\u7684\u4e8b\u7269\uff08<a href=\"\/?p=136\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"136\" title=\"\u4e09\u89c2\" target=\"_blank\">\u6587\u6863 #136<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"2\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6bb5\u843d #2<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u793e\u4ea4\u89c2<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u9010\u6e10\u8fdc\u79bb\u4e86\u793e\u4ea4\u5e73\u53f0\uff0c\u4e0d\u518d\u613f\u610f\u5206\u4eab\u81ea\u5df1\u7684\u751f\u6d3b\uff0c\u4e5f\u4e0d\u771f\u6b63\u5173\u5fc3\u670b\u53cb\u7684\u751f\u6d3b\u7ec6\u8282\uff08<a href=\"\/?p=5152\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"5152\" title=\"\u865a\u4f2a\" target=\"_blank\">\u6587\u6863 #5152<\/a>\uff0c<a href=\"\/?p=165\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"7\" title=\"\u7528\u4e8eCRISPR\u7684gRNA\u8bbe\u8ba1\" target=\"_blank\">\u6bb5\u843d #7<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u8ba4\u8bc6\u5230\u4eba\u4e0e\u4eba\u4e4b\u95f4\u7684\u8fde\u63a5\u5f88\u591a\u65f6\u5019\u4ec5\u9650\u4e8e\u4ef7\u503c\u4ea4\u6362\uff0c\u60c5\u611f\u4e5f\u662f\u4e3a\u4e86\u66f4\u65b9\u4fbf\u5730\u4ea4\u6362\uff08<a href=\"\/?p=5152\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"5152\" title=\"\u865a\u4f2a\" target=\"_blank\">\u6587\u6863 #5152<\/a>\uff0c<a href=\"\/?p=165\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"7\" title=\"\u7528\u4e8eCRISPR\u7684gRNA\u8bbe\u8ba1\" target=\"_blank\">\u6bb5\u843d #7<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u4e16\u754c\u89c2<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u5bf9\u8363\u8a89\u548c\u8bc4\u5956\u6301\u6279\u5224\u6001\u5ea6\uff0c\u8ba4\u4e3a\u8fd9\u4e9b\u5956\u9879\u662f\u7531\u89c4\u5219\u5236\u5b9a\u8005\u4eba\u4e3a\u8bbe\u7f6e\u7684\uff0c\u76ee\u7684\u662f\u4f20\u8fbe\u4e0a\u5c42\u7684\u884c\u4e3a\u89c4\u8303\u548c\u4ef7\u503c\u89c2\u5f15\u5bfc\uff08<a href=\"\/?p=8059\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"8059\" title=\"\u9c7c\u7f38\" target=\"_blank\">\u6587\u6863 #8059<\/a>\uff0c<a href=\"\/?p=8059\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"5\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #5<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u610f\u8bc6\u5230\u4e2d\u6587\u4e16\u754c\u5904\u4e8e\u73b0\u4ee3\u6587\u660e\u7684\u8fb9\u7f18\u5730\u5e26\uff0c\u4fe1\u606f\u6765\u6e90\u6709\u9650\uff08<a href=\"\/?p=4605\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"4605\" title=\"\u73b0\u4ee3\u6587\u660e\u8fb9\u7f18\u5730\u5e26\" target=\"_blank\">\u6587\u6863 #4605<\/a>\uff0c<a href=\"\/?p=4605\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"6\" title=\"\u4e09\u89c2\" target=\"_blank\">\u6bb5\u843d #6<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u4e09\u89c2\u6574\u7406<\/strong>\uff1a<\/p>\n      <ul>\n        <li>\u5728\u6574\u7406\u4e09\u89c2\u7684\u8fc7\u7a0b\u4e2d\uff0cEamon\u610f\u8bc6\u5230\u4e86\u5f88\u591a\u5e95\u5c42\u89c2\u5ff5\u7684\u4e0d\u6e05\u6670\uff0c\u4f8b\u5982\u4eba\u751f\u610f\u4e49\u3001\u7edd\u5bf9\u771f\u7406\u7684\u5b58\u5728\u6027\u3001\u79d1\u5b66\u4e0e\u54f2\u5b66\u7684\u5173\u7cfb\u7b49\uff08<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"139\" title=\"\u5f00\u609f\" target=\"_blank\">\u6587\u6863 #139<\/a>\uff0c<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"11\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #11<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u5bf9\u4e16\u754c\u7684\u770b\u6cd5\u53d8\u5f97\u66f4\u52a0\u5e73\u548c\uff0c\u4e0d\u518d\u6709\u201c\u77a7\u4e0d\u8d77\u8c01\u201d\u7684\u5fc3\u601d\uff0c\u5bf9\u81ea\u5df1\u7684\u5305\u5bb9\u5ea6\u4e5f\u589e\u52a0\u4e86\uff08<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"139\" title=\"\u5f00\u609f\" target=\"_blank\">\u6587\u6863 #139<\/a>\uff0c<a href=\"\/?p=139\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"11\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #11<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n    <li>\n      <p><strong>\u672a\u6765\u5c55\u671b<\/strong>\uff1a<\/p>\n      <ul>\n        <li>Eamon\u8bbe\u60f3\u4e94\u5e74\u540e\u6216\u5341\u5e74\u540e\u7684\u751f\u6d3b\u573a\u666f\u662f\u5728\u4e00\u4e2a\u201c\u4e0e\u4e16\u9694\u7edd\u201d\u7684\u7814\u7a76\u673a\u6784\u91cc\u6bcf\u5929\u5e73\u6de1\u5730\u505a\u7740\u81ea\u5df1\u7684\u7814\u7a76\uff0c\u6574\u7406\u548c\u5b66\u4e60\uff08<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"65\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6587\u6863 #65<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"10\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #10<\/a>\uff09\u3002<\/li>\n        <li>\u4ed6\u5bf9\u5b66\u672f\u6c14\u6c1b\u4e0d\u6ee1\u610f\uff0c\u8868\u793a\u5982\u679c\u8feb\u4e8e\u73b0\u5b9e\u538b\u529b\u8981\u505a\u51fa\u8ba9\u672c\u79d1\u65f6\u7684\u81ea\u5df1\u8ba8\u538c\u7684\u884c\u4e3a\uff0c\u4f1a\u9009\u62e9\u9000\u51fa\u884c\u4e1a\u6765\u4fdd\u5168\u5fc3\u4e2d\u7684\u5723\u5730\uff08<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"document\" data-id=\"65\" title=\"\u4e0e\u5b8b\u540c\u5b66\u7684\u6bd5\u4e1a\u544a\u522b\u8c08\u8bdd\" target=\"_blank\">\u6587\u6863 #65<\/a>\uff0c<a href=\"\/?p=65\" class=\"ai-search-reference\" data-reference-type=\"paragraph\" data-id=\"10\" title=\"\u6765\u6e90\u6587\u7ae0\" target=\"_blank\">\u6bb5\u843d #10<\/a>\uff09\u3002<\/li>\n      <\/ul>\n    <\/li>\n  <\/ol>\n  <p>\u7efc\u4e0a\u6240\u8ff0\uff0cEamon\u662f\u4e00\u4e2a\u5bf9\u751f\u6d3b\u6709\u7740\u6df1\u523b\u601d\u8003\u7684\u4eba\uff0c\u4ed6\u5728\u8ffd\u6c42\u79d1\u5b66\u7814\u7a76\u7684\u540c\u65f6\uff0c\u4e5f\u5728\u4e0d\u65ad\u63a2\u7d22\u81ea\u6211\u548c\u4e16\u754c\u7684\u672c\u8d28\u3002\u4ed6\u5bf9\u5f85\u751f\u6d3b\u548c\u5de5\u4f5c\u7684\u6001\u5ea6\u8f83\u4e3a\u4f5b\u7cfb\uff0c\u6ce8\u91cd\u4f53\u9a8c\u800c\u975e\u76ee\u6807\uff0c\u540c\u65f6\u5bf9\u793e\u4f1a\u73b0\u8c61\u6709\u7740\u72ec\u7279\u7684\u89c1\u89e3\u548c\u6279\u5224\u7cbe\u795e\u3002<\/p>\n<\/div>\n\n\n\n<p>\u7136\u540e\u5168\u6587\u68c0\u7d22\uff1a<\/p>\n\n\n\n<p>\u7ed3\u679c\u4e0d\u77e5\u9053\u662f\u4ec0\u4e48\u539f\u56e0\uff0cresponse\u662f\u7a7a\u5185\u5bb9\uff0c\u76f4\u63a5\u5c31done\u4e86\uff0c\u4e5f\u4e0d\u7ed9\u9519\u8bef\u7801\u3002\u6240\u4ee5\u76ee\u524d\u5c31\u53ea\u6709\u4e00\u4e2aQwen-long\u7684\u5b64\u4f8b\u3002<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<p>\u8fd8\u8bd5\u4e86\u4e0bGemini\uff0c\u4e0d\u8fc7\u6211\u7684API\u9650\u989d\u4e86\uff0cChatGPT\u957f\u4e0a\u4e0b\u6587\u7684\u53ea\u6709\u4e00\u4e2aGPT4.1\uff0c\u4f46\u662f\u72d7\u903cOpenAI\u82b1\u94b1\u90fd\u4e0d\u884c\uff0c\u8fd8\u5f97\u6d88\u8d39\u8fbe\u5230\u4e00\u5b9a\u6570\u91cf\u81ea\u52a8\u5347\u7ea7\u4e0a\u4e0b\u6587\u957f\u5ea6\uff0c\u53ea\u5f97\u4f5c\u7f62\u3002<\/p>\n<\/blockquote>\n\n\n\n<p>\u603b\u4e4b\u76ee\u524d\u7684\u5b64\u4f8b\u6253\u6d88\u4e86\u6211\u5bf9\u957f\u4e0a\u4e0b\u6587\u7684\u4e0d\u5207\u5b9e\u9645\u7684\u671f\u5f85\uff0c\u5b9e\u8df5\u4e0b\u6765\u8fd8\u662fRAG\u7269\u7f8e\u4ef7\u5ec9\u4e00\u4e9b\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\" \/>\n\n\n\n<p class=\"has-d-8-dee-9-ff-color has-text-color\">\u9644\u4e0a\u672c\u6587\u4e24\u79cd\u65b9\u6cd5\u7684\u63d0\u793a\u8bcd\u793a\u4f8b\uff1a<\/p>\n\n\n\n<div class=\"wp-block-kevinbatdorf-code-block-pro cbp-has-line-numbers\" data-code-block-pro-font-family=\"Code-Pro-JetBrains-Mono\" style=\"font-size:.875rem;font-family:Code-Pro-JetBrains-Mono,ui-monospace,SFMono-Regular,Menlo,Monaco,Consolas,monospace;--cbp-line-number-color:#d8dee9ff;--cbp-line-number-width:calc(2 * 0.6 * .875rem);line-height:1.25rem;--cbp-tab-width:2\"><span style=\"align-items:center;padding:10px 0px 10px 16px;margin-bottom:-2px;width:100%;text-align:left;background-color:#39404f;color:#c8d0e0\">\u5411\u91cf\u641c\u7d22<\/span><span role=\"button\" data-code=\"\u8bf7\u57fa\u4e8e\u4ee5\u4e0b\u641c\u7d22\u8bcd\u548c\u8bed\u4e49\u76f8\u5173\u7684\u5185\u5bb9\u63d0\u4f9b\u8be6\u7ec6\u7b54\u6848\uff1aCRISPR \u6570\u636e\u5206\u6790\n\n\u4ee5\u4e0b\u662f\u4e0e\u641c\u7d22\u8bcd\u8bed\u4e49\u6700\u76f8\u5173\u7684\u7f51\u7ad9\u5185\u5bb9\u6bb5\u843d\uff1a\n\n\u6bb5\u843d #0\uff1a\nCRISPR \u6280\u672f\u5e7f\u6cdb\u7528\u4e8e\u57fa\u56e0\u7f16\u8f91\u548c\u529f\u80fd\u7b5b\u9009\uff0c\u6570\u636e\u5206\u6790\u9700\u8981\u5305\u542b reads \u5bf9\u9f50\u3001\u7a81\u53d8\u7387\u7edf\u8ba1\u548c\u5bcc\u96c6\u5206\u6790\u7b49\u6b65\u9aa4\u3002\n\uff08\u6765\u6e90\uff1aCRISPR\u7b5b\u9009\u6559\u7a0b\uff0c\u6587\u6863 #1023\uff0c\u5206\u7c7b\uff1a\u57fa\u56e0\u7f16\u8f91\uff0c\u4f5c\u8005\uff1a\u674e\u96f7\uff0c\u65e5\u671f\uff1a2024-10-15\uff09\n\n\u6bb5\u843d #1\uff1a\n\u6570\u636e\u8d28\u91cf\u63a7\u5236\u662f CRISPR \u6570\u636e\u5206\u6790\u7684\u7b2c\u4e00\u6b65\uff0c\u5efa\u8bae\u4f7f\u7528 Cutadapt\u3001FastQC \u7b49\u5de5\u5177\u8fdb\u884c\u524d\u5904\u7406\u3002\n\uff08\u6765\u6e90\uff1aNGS\u6570\u636e\u9884\u5904\u7406\uff0c\u6587\u6863 #998\uff0c\u5206\u7c7b\uff1a\u6559\u7a0b\uff0c\u4f5c\u8005\uff1a\u738b\u82b3\uff0c\u65e5\u671f\uff1a2024-09-30\uff09\n\n\u6bb5\u843d #2\uff1a\n\u5bf9\u4e8e\u5bcc\u96c6\u5206\u6790\uff0c\u53ef\u4f7f\u7528 MAGeCK \u6216 PinAPL-Py \u7b49\u8f6f\u4ef6\uff0c\u5bf9 guideRNA \u4e30\u5bcc\u5ea6\u53d8\u5316\u8fdb\u884c\u7edf\u8ba1\u68c0\u9a8c\u3002\n\uff08\u6765\u6e90\uff1a\u529f\u80fd\u7b5b\u9009\u5206\u6790\u6307\u5357\uff0c\u6587\u6863 #1105\uff0c\u5206\u7c7b\uff1a\u751f\u7269\u4fe1\u606f\uff0c\u4f5c\u8005\uff1a\u8d75\u521a\uff0c\u65e5\u671f\uff1a2025-01-20\uff09\n\n\u8bf7\u6839\u636e\u4ee5\u4e0a\u5185\u5bb9\uff0c\u4e3a\u7528\u6237\u63d0\u4f9b\u6709\u5173\u201cCRISPR \u6570\u636e\u5206\u6790\u201d\u7684\u8be6\u7ec6\u4fe1\u606f\u3002\u5982\u679c\u5185\u5bb9\u4e2d\u6ca1\u6709\u76f8\u5173\u4fe1\u606f\uff0c\u8bf7\u660e\u786e\u544a\u77e5\u7528\u6237\u3002\n\n\u5f15\u7528\u683c\u5f0f\u8bf4\u660e\uff1a\n1. \u6587\u6863\u5f15\u7528\uff1a\u6587\u6863 #ID\uff08\u5982\uff1a\u6587\u6863 #9680\uff09\n2. \u6bb5\u843d\u5f15\u7528\uff1a\u6bb5\u843d #ID\uff08\u5982\uff1a\u6bb5\u843d #2\uff0c\u4e0d\u53ef\u7b80\u5199\uff09\n3. \u591a\u6bb5\u843d\u5f15\u7528\uff1a\u6bb5\u843d #1\u3001\u6bb5\u843d #2\u3001\u6bb5\u843d #3\n4. \u6587\u7ae0\u4fe1\u606f\uff1a\u5206\u7c7b\u3001\u4f5c\u8005\u3001\u65e5\u671f\uff08\u5982\uff1a'\u6559\u7a0b'\u5206\u7c7b\uff0c\u4f5c\u8005'\u5f20\u4e09'\uff0c2023-01-15\uff09\n\u5f15\u7528\u548c\u6587\u7ae0\u4fe1\u606f\u5fc5\u987b\u6dfb\u52a0\uff0c\u8bf7\u7d27\u8ddf\u5f15\u7528\u5185\u5bb9\u540e\u3002\n\" style=\"color:#d8dee9ff\" aria-label=\"\u590d\u5236\" class=\"code-block-pro-copy-button\"><\/span><pre class=\"shiki nord\" style=\"background-color: #2e3440ff\"><code><span class=\"line\"><span style=\"color: #D8DEE9\">\u8bf7\u57fa\u4e8e\u4ee5\u4e0b\u641c\u7d22\u8bcd\u548c\u8bed\u4e49\u76f8\u5173\u7684\u5185\u5bb9\u63d0\u4f9b\u8be6\u7ec6\u7b54\u6848<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">CRISPR<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6570\u636e\u5206\u6790<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u4ee5\u4e0b\u662f\u4e0e\u641c\u7d22\u8bcd\u8bed\u4e49\u6700\u76f8\u5173\u7684\u7f51\u7ad9\u5185\u5bb9\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">0<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">CRISPR<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6280\u672f\u5e7f\u6cdb\u7528\u4e8e\u57fa\u56e0\u7f16\u8f91\u548c\u529f\u80fd\u7b5b\u9009<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u6570\u636e\u5206\u6790\u9700\u8981\u5305\u542b<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">reads<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u5bf9\u9f50<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u7a81\u53d8\u7387\u7edf\u8ba1\u548c\u5bcc\u96c6\u5206\u6790\u7b49\u6b65\u9aa4<\/span><span style=\"color: #D8DEE9FF\">\u3002<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">\uff08<\/span><span style=\"color: #D8DEE9\">\u6765\u6e90<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">CRISPR\u7b5b\u9009\u6559\u7a0b<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u6587\u6863<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">1023<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u5206\u7c7b<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u57fa\u56e0\u7f16\u8f91<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u4f5c\u8005<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u674e\u96f7<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u65e5\u671f<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #B48EAD\">2024<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">10<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">15<\/span><span style=\"color: #D8DEE9FF\">\uff09<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">1<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u6570\u636e\u8d28\u91cf\u63a7\u5236\u662f<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">CRISPR<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6570\u636e\u5206\u6790\u7684\u7b2c\u4e00\u6b65<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u5efa\u8bae\u4f7f\u7528<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">Cutadapt<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">FastQC<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u7b49\u5de5\u5177\u8fdb\u884c\u524d\u5904\u7406<\/span><span style=\"color: #D8DEE9FF\">\u3002<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">\uff08<\/span><span style=\"color: #D8DEE9\">\u6765\u6e90<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">NGS\u6570\u636e\u9884\u5904\u7406<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u6587\u6863<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">998<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u5206\u7c7b<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6559\u7a0b<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u4f5c\u8005<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u738b\u82b3<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u65e5\u671f<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #B48EAD\">2024<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">09<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">30<\/span><span style=\"color: #D8DEE9FF\">\uff09<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">2<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u5bf9\u4e8e\u5bcc\u96c6\u5206\u6790<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u53ef\u4f7f\u7528<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">MAGeCK<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6216<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">PinAPL<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #D8DEE9\">Py<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u7b49\u8f6f\u4ef6<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u5bf9<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">guideRNA<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u4e30\u5bcc\u5ea6\u53d8\u5316\u8fdb\u884c\u7edf\u8ba1\u68c0\u9a8c<\/span><span style=\"color: #D8DEE9FF\">\u3002<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9FF\">\uff08<\/span><span style=\"color: #D8DEE9\">\u6765\u6e90<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u529f\u80fd\u7b5b\u9009\u5206\u6790\u6307\u5357<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u6587\u6863<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">1105<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u5206\u7c7b<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u751f\u7269\u4fe1\u606f<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u4f5c\u8005<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u8d75\u521a<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u65e5\u671f<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #B48EAD\">2025<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">01<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">20<\/span><span style=\"color: #D8DEE9FF\">\uff09<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u8bf7\u6839\u636e\u4ee5\u4e0a\u5185\u5bb9<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u4e3a\u7528\u6237\u63d0\u4f9b\u6709\u5173<\/span><span style=\"color: #D8DEE9FF\">\u201c<\/span><span style=\"color: #D8DEE9\">CRISPR<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6570\u636e\u5206\u6790<\/span><span style=\"color: #D8DEE9FF\">\u201d<\/span><span style=\"color: #D8DEE9\">\u7684\u8be6\u7ec6\u4fe1\u606f<\/span><span style=\"color: #D8DEE9FF\">\u3002<\/span><span style=\"color: #D8DEE9\">\u5982\u679c\u5185\u5bb9\u4e2d\u6ca1\u6709\u76f8\u5173\u4fe1\u606f<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u8bf7\u660e\u786e\u544a\u77e5\u7528\u6237<\/span><span style=\"color: #D8DEE9FF\">\u3002<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u5f15\u7528\u683c\u5f0f\u8bf4\u660e<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><\/span>\n<span class=\"line\"><span style=\"color: #B48EAD\">1.<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6587\u6863\u5f15\u7528<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6587\u6863<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #D8DEE9\">ID<\/span><span style=\"color: #D8DEE9FF\">\uff08<\/span><span style=\"color: #D8DEE9\">\u5982<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6587\u6863<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">9680<\/span><span style=\"color: #D8DEE9FF\">\uff09<\/span><\/span>\n<span class=\"line\"><span style=\"color: #B48EAD\">2.<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d\u5f15\u7528<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #D8DEE9\">ID<\/span><span style=\"color: #D8DEE9FF\">\uff08<\/span><span style=\"color: #D8DEE9\">\u5982<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">2<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u4e0d\u53ef\u7b80\u5199<\/span><span style=\"color: #D8DEE9FF\">\uff09<\/span><\/span>\n<span class=\"line\"><span style=\"color: #B48EAD\">3.<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u591a\u6bb5\u843d\u5f15\u7528<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">1<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">2<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">3<\/span><\/span>\n<span class=\"line\"><span style=\"color: #B48EAD\">4.<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6587\u7ae0\u4fe1\u606f<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u5206\u7c7b<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u4f5c\u8005<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u65e5\u671f<\/span><span style=\"color: #D8DEE9FF\">\uff08<\/span><span style=\"color: #D8DEE9\">\u5982<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #ECEFF4\">&#039;<\/span><span style=\"color: #A3BE8C\">\u6559\u7a0b<\/span><span style=\"color: #ECEFF4\">&#039;<\/span><span style=\"color: #D8DEE9\">\u5206\u7c7b<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u4f5c\u8005<\/span><span style=\"color: #ECEFF4\">&#039;<\/span><span style=\"color: #A3BE8C\">\u5f20\u4e09<\/span><span style=\"color: #ECEFF4\">&#039;<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #B48EAD\">2023<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">01<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">15<\/span><span style=\"color: #D8DEE9FF\">\uff09<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u5f15\u7528\u548c\u6587\u7ae0\u4fe1\u606f\u5fc5\u987b\u6dfb\u52a0<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u8bf7\u7d27\u8ddf\u5f15\u7528\u5185\u5bb9\u540e<\/span><span style=\"color: #D8DEE9FF\">\u3002<\/span><\/span>\n<span class=\"line\"><\/span><\/code><\/pre><\/div>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-kevinbatdorf-code-block-pro cbp-has-line-numbers\" data-code-block-pro-font-family=\"Code-Pro-JetBrains-Mono\" style=\"font-size:.875rem;font-family:Code-Pro-JetBrains-Mono,ui-monospace,SFMono-Regular,Menlo,Monaco,Consolas,monospace;--cbp-line-number-color:#d8dee9ff;--cbp-line-number-width:calc(2 * 0.6 * .875rem);line-height:1.25rem;--cbp-tab-width:2\"><span style=\"align-items:center;padding:10px 0px 10px 16px;margin-bottom:-2px;width:100%;text-align:left;background-color:#39404f;color:#c8d0e0\">\u5168\u6587\u641c\u7d22<\/span><span role=\"button\" data-code=\"\u8bf7\u6839\u636e\u4ee5\u4e0b\u7f51\u7ad9\u5185\u5bb9\uff0c\u4e3a\u7528\u6237\u63d0\u4f9b\u5173\u4e8e\u201cCRISPR \u6570\u636e\u5206\u6790\u201d\u7684\u7cbe\u786e\u3001\u6df1\u5165\u89e3\u7b54\u3002\u8bf7\u81f3\u5c11\u5217\u51fa\u4e09\u70b9\u76f8\u5173\u8981\u70b9\uff0c\u5e76\u6ce8\u660e\u5f15\u7528\u7684\u6587\u6863\u7f16\u53f7\u3002\n\n\u4ee5\u4e0b\u662f\u7f51\u7ad9\u4e2d\u4e0e\u201cCRISPR \u6570\u636e\u5206\u6790\u201d\u53ef\u80fd\u76f8\u5173\u7684\u6587\u6863\u6458\u8981\u5185\u5bb9\uff1a\n\n\u6587\u6863 #1023\uff1aCRISPR\u7b5b\u9009\u6559\u7a0b  \n\u6458\u8981\uff1aCRISPR \u6280\u672f\u5e7f\u6cdb\u7528\u4e8e\u57fa\u56e0\u7f16\u8f91\u548c\u529f\u80fd\u7b5b\u9009\uff0c\u6570\u636e\u5206\u6790\u6b65\u9aa4\u5305\u62ec reads \u5bf9\u9f50\u3001\u7a81\u53d8\u7387\u7edf\u8ba1\u3001\u5bcc\u96c6\u5206\u6790\u7b49\u2026\u2026  \n\u5206\u7c7b\uff1a\u57fa\u56e0\u7f16\u8f91\uff1b\u4f5c\u8005\uff1a\u674e\u96f7\uff1b\u65e5\u671f\uff1a2024-10-15\n\n\u6587\u6863 #998\uff1aNGS\u6570\u636e\u9884\u5904\u7406  \n\u6458\u8981\uff1a\u5206\u6790\u524d\u9700\u8981\u4f7f\u7528 Cutadapt\u3001FastQC \u7b49\u5de5\u5177\u6e05\u6d17\u539f\u59cb\u6d4b\u5e8f\u6570\u636e\uff0c\u786e\u4fdd\u6570\u636e\u8d28\u91cf\u2026\u2026  \n\u5206\u7c7b\uff1a\u6559\u7a0b\uff1b\u4f5c\u8005\uff1a\u738b\u82b3\uff1b\u65e5\u671f\uff1a2024-09-30\n\n\u6587\u6863 #1105\uff1a\u529f\u80fd\u7b5b\u9009\u5206\u6790\u6307\u5357  \n\u6458\u8981\uff1a\u5728\u5b8c\u6210\u57fa\u7840\u8d28\u63a7\u540e\uff0c\u5bcc\u96c6\u5206\u6790\u53ef\u4f7f\u7528 MAGeCK \u6216 PinAPL-Py \u7b49\u8f6f\u4ef6\uff0c\u5bf9 guideRNA \u4e30\u5bcc\u5ea6\u8fdb\u884c\u7edf\u8ba1\u68c0\u9a8c\u2026\u2026  \n\u5206\u7c7b\uff1a\u751f\u7269\u4fe1\u606f\uff1b\u4f5c\u8005\uff1a\u8d75\u521a\uff1b\u65e5\u671f\uff1a2025-01-20\n\n\u8bf7\u57fa\u4e8e\u4ee5\u4e0a\u5185\u5bb9\uff0c\u4ece\u4e2d\u5f52\u7eb3\u603b\u7ed3\u4e0e\u201cCRISPR \u6570\u636e\u5206\u6790\u201d\u6700\u76f8\u5173\u7684\u4fe1\u606f\u3002\u5982\u672a\u80fd\u627e\u5230\uff0c\u8bf7\u660e\u786e\u544a\u77e5\u7528\u6237\u5185\u5bb9\u672a\u6db5\u76d6\u76f8\u5173\u4e3b\u9898\u3002\n\n\u5f15\u7528\u683c\u5f0f\u8bf4\u660e\uff1a\n1. \u6587\u6863\u5f15\u7528\uff1a\u6587\u6863 #ID\uff08\u5982\uff1a\u6587\u6863 #9680\uff09\n2. \u6bb5\u843d\u5f15\u7528\uff1a\u6bb5\u843d #ID\uff08\u5982\uff1a\u6bb5\u843d #2\uff0c\u4e0d\u53ef\u7b80\u5199\uff09\n3. \u591a\u6bb5\u843d\u5f15\u7528\uff1a\u6bb5\u843d #1\u3001\u6bb5\u843d #2\u3001\u6bb5\u843d #3\n4. \u6587\u7ae0\u4fe1\u606f\uff1a\u5206\u7c7b\u3001\u4f5c\u8005\u3001\u65e5\u671f\uff08\u5982\uff1a'\u6559\u7a0b'\u5206\u7c7b\uff0c\u4f5c\u8005'\u5f20\u4e09'\uff0c2023-01-15\uff09\n\u5f15\u7528\u548c\u6587\u7ae0\u4fe1\u606f\u5fc5\u987b\u6dfb\u52a0\uff0c\u8bf7\u7d27\u8ddf\u5f15\u7528\u5185\u5bb9\u540e\u3002\" style=\"color:#d8dee9ff\" aria-label=\"\u590d\u5236\" class=\"code-block-pro-copy-button\"><\/span><pre class=\"shiki nord\" style=\"background-color: #2e3440ff\"><code><span class=\"line\"><span style=\"color: #D8DEE9\">\u8bf7\u6839\u636e\u4ee5\u4e0b\u7f51\u7ad9\u5185\u5bb9<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u4e3a\u7528\u6237\u63d0\u4f9b\u5173\u4e8e<\/span><span style=\"color: #D8DEE9FF\">\u201c<\/span><span style=\"color: #D8DEE9\">CRISPR<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6570\u636e\u5206\u6790<\/span><span style=\"color: #D8DEE9FF\">\u201d<\/span><span style=\"color: #D8DEE9\">\u7684\u7cbe\u786e<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u6df1\u5165\u89e3\u7b54<\/span><span style=\"color: #D8DEE9FF\">\u3002<\/span><span style=\"color: #D8DEE9\">\u8bf7\u81f3\u5c11\u5217\u51fa\u4e09\u70b9\u76f8\u5173\u8981\u70b9<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u5e76\u6ce8\u660e\u5f15\u7528\u7684\u6587\u6863\u7f16\u53f7<\/span><span style=\"color: #D8DEE9FF\">\u3002<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u4ee5\u4e0b\u662f\u7f51\u7ad9\u4e2d\u4e0e<\/span><span style=\"color: #D8DEE9FF\">\u201c<\/span><span style=\"color: #D8DEE9\">CRISPR<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6570\u636e\u5206\u6790<\/span><span style=\"color: #D8DEE9FF\">\u201d<\/span><span style=\"color: #D8DEE9\">\u53ef\u80fd\u76f8\u5173\u7684\u6587\u6863\u6458\u8981\u5185\u5bb9<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u6587\u6863<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">1023<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">CRISPR\u7b5b\u9009\u6559\u7a0b<\/span><span style=\"color: #D8DEE9FF\">  <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u6458\u8981<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">CRISPR<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6280\u672f\u5e7f\u6cdb\u7528\u4e8e\u57fa\u56e0\u7f16\u8f91\u548c\u529f\u80fd\u7b5b\u9009<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u6570\u636e\u5206\u6790\u6b65\u9aa4\u5305\u62ec<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">reads<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u5bf9\u9f50<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u7a81\u53d8\u7387\u7edf\u8ba1<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u5bcc\u96c6\u5206\u6790\u7b49<\/span><span style=\"color: #D8DEE9FF\">\u2026\u2026  <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u5206\u7c7b<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u57fa\u56e0\u7f16\u8f91<\/span><span style=\"color: #D8DEE9FF\">\uff1b<\/span><span style=\"color: #D8DEE9\">\u4f5c\u8005<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u674e\u96f7<\/span><span style=\"color: #D8DEE9FF\">\uff1b<\/span><span style=\"color: #D8DEE9\">\u65e5\u671f<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #B48EAD\">2024<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">10<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">15<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u6587\u6863<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">998<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">NGS\u6570\u636e\u9884\u5904\u7406<\/span><span style=\"color: #D8DEE9FF\">  <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u6458\u8981<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u5206\u6790\u524d\u9700\u8981\u4f7f\u7528<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">Cutadapt<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">FastQC<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u7b49\u5de5\u5177\u6e05\u6d17\u539f\u59cb\u6d4b\u5e8f\u6570\u636e<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u786e\u4fdd\u6570\u636e\u8d28\u91cf<\/span><span style=\"color: #D8DEE9FF\">\u2026\u2026  <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u5206\u7c7b<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6559\u7a0b<\/span><span style=\"color: #D8DEE9FF\">\uff1b<\/span><span style=\"color: #D8DEE9\">\u4f5c\u8005<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u738b\u82b3<\/span><span style=\"color: #D8DEE9FF\">\uff1b<\/span><span style=\"color: #D8DEE9\">\u65e5\u671f<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #B48EAD\">2024<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">09<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">30<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u6587\u6863<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">1105<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u529f\u80fd\u7b5b\u9009\u5206\u6790\u6307\u5357<\/span><span style=\"color: #D8DEE9FF\">  <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u6458\u8981<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u5728\u5b8c\u6210\u57fa\u7840\u8d28\u63a7\u540e<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u5bcc\u96c6\u5206\u6790\u53ef\u4f7f\u7528<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">MAGeCK<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6216<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">PinAPL<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #D8DEE9\">Py<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u7b49\u8f6f\u4ef6<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u5bf9<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">guideRNA<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u4e30\u5bcc\u5ea6\u8fdb\u884c\u7edf\u8ba1\u68c0\u9a8c<\/span><span style=\"color: #D8DEE9FF\">\u2026\u2026  <\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u5206\u7c7b<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u751f\u7269\u4fe1\u606f<\/span><span style=\"color: #D8DEE9FF\">\uff1b<\/span><span style=\"color: #D8DEE9\">\u4f5c\u8005<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u8d75\u521a<\/span><span style=\"color: #D8DEE9FF\">\uff1b<\/span><span style=\"color: #D8DEE9\">\u65e5\u671f<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #B48EAD\">2025<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">01<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">20<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u8bf7\u57fa\u4e8e\u4ee5\u4e0a\u5185\u5bb9<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u4ece\u4e2d\u5f52\u7eb3\u603b\u7ed3\u4e0e<\/span><span style=\"color: #D8DEE9FF\">\u201c<\/span><span style=\"color: #D8DEE9\">CRISPR<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6570\u636e\u5206\u6790<\/span><span style=\"color: #D8DEE9FF\">\u201d<\/span><span style=\"color: #D8DEE9\">\u6700\u76f8\u5173\u7684\u4fe1\u606f<\/span><span style=\"color: #D8DEE9FF\">\u3002<\/span><span style=\"color: #D8DEE9\">\u5982\u672a\u80fd\u627e\u5230<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u8bf7\u660e\u786e\u544a\u77e5\u7528\u6237\u5185\u5bb9\u672a\u6db5\u76d6\u76f8\u5173\u4e3b\u9898<\/span><span style=\"color: #D8DEE9FF\">\u3002<\/span><\/span>\n<span class=\"line\"><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u5f15\u7528\u683c\u5f0f\u8bf4\u660e<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><\/span>\n<span class=\"line\"><span style=\"color: #B48EAD\">1.<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6587\u6863\u5f15\u7528<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6587\u6863<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #D8DEE9\">ID<\/span><span style=\"color: #D8DEE9FF\">\uff08<\/span><span style=\"color: #D8DEE9\">\u5982<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6587\u6863<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">9680<\/span><span style=\"color: #D8DEE9FF\">\uff09<\/span><\/span>\n<span class=\"line\"><span style=\"color: #B48EAD\">2.<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d\u5f15\u7528<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #D8DEE9\">ID<\/span><span style=\"color: #D8DEE9FF\">\uff08<\/span><span style=\"color: #D8DEE9\">\u5982<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">2<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u4e0d\u53ef\u7b80\u5199<\/span><span style=\"color: #D8DEE9FF\">\uff09<\/span><\/span>\n<span class=\"line\"><span style=\"color: #B48EAD\">3.<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u591a\u6bb5\u843d\u5f15\u7528<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">1<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">2<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u6bb5\u843d<\/span><span style=\"color: #D8DEE9FF\"> #<\/span><span style=\"color: #B48EAD\">3<\/span><\/span>\n<span class=\"line\"><span style=\"color: #B48EAD\">4.<\/span><span style=\"color: #D8DEE9FF\"> <\/span><span style=\"color: #D8DEE9\">\u6587\u7ae0\u4fe1\u606f<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #D8DEE9\">\u5206\u7c7b<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u4f5c\u8005<\/span><span style=\"color: #D8DEE9FF\">\u3001<\/span><span style=\"color: #D8DEE9\">\u65e5\u671f<\/span><span style=\"color: #D8DEE9FF\">\uff08<\/span><span style=\"color: #D8DEE9\">\u5982<\/span><span style=\"color: #D8DEE9FF\">\uff1a<\/span><span style=\"color: #ECEFF4\">&#039;<\/span><span style=\"color: #A3BE8C\">\u6559\u7a0b<\/span><span style=\"color: #ECEFF4\">&#039;<\/span><span style=\"color: #D8DEE9\">\u5206\u7c7b<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u4f5c\u8005<\/span><span style=\"color: #ECEFF4\">&#039;<\/span><span style=\"color: #A3BE8C\">\u5f20\u4e09<\/span><span style=\"color: #ECEFF4\">&#039;<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #B48EAD\">2023<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">01<\/span><span style=\"color: #81A1C1\">-<\/span><span style=\"color: #B48EAD\">15<\/span><span style=\"color: #D8DEE9FF\">\uff09<\/span><\/span>\n<span class=\"line\"><span style=\"color: #D8DEE9\">\u5f15\u7528\u548c\u6587\u7ae0\u4fe1\u606f\u5fc5\u987b\u6dfb\u52a0<\/span><span style=\"color: #D8DEE9FF\">\uff0c<\/span><span style=\"color: #D8DEE9\">\u8bf7\u7d27\u8ddf\u5f15\u7528\u5185\u5bb9\u540e<\/span><span style=\"color: #D8DEE9FF\">\u3002<\/span><\/span><\/code><\/pre><\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4e3b\u4eba\u6298\u817e\u4e86\u4e00\u663c\u591c\uff0c\u7ed9\u7f51\u7ad9\u52a0\u4e0a\u4e86AI\u641c\u7d22\u529f\u80fd\uff0c\u8fd8\u987a\u4fbf\u5b9e\u8df5\u4e86\u957f\u4e0a\u4e0b\u6587\u4e0eRAG\u7684\u5bf9\u6bd4\u3002\u4ed6\u7528\u201c\u535a\u4e3b\u662f\u4e2a\u600e\u6837\u7684\u4eba\uff1f\u201d\u8fd9\u4e2a\u95ee\u9898\uff0c\u8ba9DeepSeek\u3001Qwen-long\u548cGLM-4-Long\u51e0\u4e2a\u6a21\u578b\u8f6e\u756a\u4e0a\u9635\uff0c\u7ed3\u679c\u561b\u2026\u2026\u6709\u70b9\u51fa\u4e4e\u610f\u6599\u54e6\u3002<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"class_list":["post-9947","post","type-post","status-publish","format-standard","hentry","category-blog"],"_links":{"self":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/9947","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9947"}],"version-history":[{"count":2,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/9947\/revisions"}],"predecessor-version":[{"id":13860,"href":"https:\/\/fanyiming.life\/index.php?rest_route=\/wp\/v2\/posts\/9947\/revisions\/13860"}],"wp:attachment":[{"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9947"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9947"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fanyiming.life\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9947"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}