RTCB的two-step ligation

Tanaka N, Chakravarty AK, Maughan B, Shuman S. Novel mechanism of RNA repair by RtcB via sequential 2′,3′-cyclic phosphodiesterase and 3′-Phosphate/5′-hydroxyl ligation reactions. J Biol Chem. 2011 Dec 16;286(50):43134-43. doi: 10.1074/jbc.M111.302133. Epub 2011 Oct 31. PMID: 22045815; PMCID: PMC3234866.

RTCB同时含有CPDase活性和3′-P ligase活性，意味着RTCB的底物不只是2′,3′-cyclic phosphate，还包括3′-phosphate。

后面一篇Science又证实了human RtcB可以连接3’P和5′-OH.