https://pubmed.ncbi.nlm.nih.gov/32494599

文章大意

这篇文章发现核内肌动蛋白（nuclear actin）在诱导性转录中通过促进RNA聚合酶II（Pol II）聚集来调节基因表达，具体机制涉及N-WASP和Arp2/3复合体介导的actin聚合，并在血清和IFN-γ刺激下均表现出增强Pol II聚集和转录活性的作用，揭示了nuclear actin在细胞快速响应环境变化中的关键功能。

主要结果

通过RNA测序分析发现，破坏nuclear actin显著降低了血清响应基因的表达

Fig. 1. Nuclear actin is required for the establishment of the serum-inducedtranscriptional program. (A) Heatmap showing the fold change for differentiallyexpressed genes upon serum stimulation in cells overexpressing G13R and cellsoverexpressing GFP. (B) Venn diagrams showing the numbers of differentially ex-pressed genes. (C) qRT-PCR conformation for nine canonical serum-response genesand two housekeeping genes. (D) The functional annotations of the top-rankedgenes after sorting by the level of transcriptional change caused by actin mutantoverexpression.